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User Guide

1. Options x 4 General Default Folders Scale Bar Type Profiles Image Names amp Saving Scale Bar Type Line Segment z Save Sound E e Scale Bar Size Do Not Show This Window Annotations Scale Bar Size Small Auto Size M e 10 Hm Appearance and Layout Miscellaneous Preview 4 Measurements 5 Units pS Scale Bar Sizes Colors Other Settings Scale Bar Options 4 Hardware Imaging Devices Color of Scale Bar and its Value Change Soom ce Background Area Value and Scale Bar Background Color Change V Display Scale Bar Value Automatic Scale Bar Insertion Insert Scale Bar into Image Automatically after Magnification Assignment AlBackup settings ai Loadsettings Configuration wizard ok cancel Tab Sizes Colors On this tab you may set the font size of the scale bar s value This setting will become valid for all newly acquired images 90 Main Program Window Options x 4 General All settings on this tab represent default values valid for all new images Default Folders Image Names amp Saving Sound Seems Font Size of Scale Bar Value Do Not Show This Window eaten Font Size 12 Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger E Backup Settings fa Load Settings Configuration Wizard OK Cancel Tab Othe
2. Default JPEG Quality Quality 35 High a a Small File Large File Gel Bedup settings i Loadsettings configuration Wizard o cna In the Store Images after Acquisition in part it is possible to select one of three options where the acquired images are supposed to be stored Program Only new images will after acquiring be displayed in the Main Program Window Saving of images will be fully in the hands of the user Program and Folder Automatic Saving if you activate this option acquired images will be displayed in the Main Program Window and at the same time they will automatically be saved into the folder selected in the Folder for Automatic Image Saving part For this function a folder selected as the default folder for image saving is set in the default state Active mode for automatic image saving will be indicated by the icon in the status bar in the right bottom corner of the program If you move the mouse over this icon information about the folder where the images are being saved will be displayed Folder Only Automatic Saving if you activate this options newly acquired images will be automatically saved to a folder selected in the Folder for Automatic Image Saving part and they will not show in the Main Program Window at all For this function 80 Main Program Window a folder selected as the default folder for image saving is set in the
3. Configuration Wizard x QuickPHOTO lor Theme In this step a program color theme can be selected 10 Initial Configuration of the Program Antialiasing of Inserted Objects On this page of the wizard you may enable the antialiasing of inserted objects feature This feature is disabled in a default state To continue click the Next button lt gt Configuration Wizard SE Qui ckPHOTO Antialiasing of Inserted Objects This function enables smoothing of inserted objects edges however it influences the display DS L R 3 1 performance For low performance computers it is recommended to keep this function disabled Finish the configuration by clicking the Finish button QuickPHOTO DSLR 3 1 program will then run automatically Initial Configuration of the Program 12 Entering License Information After the installation the QuickPHOTO DSLR 3 1 program starts in a mode of a trial version and a welcome screen appears QuickPHOTO DSLR 3 1 gt Welcome to Trial Version of QuickPHOTO DSLR 3 1 A trial version offers the evaluation of work with all functions of QuickPHOTO DSLR 3 1 program without any limitation for a period of 30 days im Continue Trial 30 Days Remaining Purchase Visit Homepage gw Enter License Information The QuickPHOTO DSLR 3 1 program can be used in trial mode without any functional limitation for a time per
4. Image Metadata Calibration Image Metadata Calibration a Path D mages Property Name cod Orientation Normal Name sample 1 jpg ISO Speed Rating 100 Size 959KB X Resolution 72 F Y Resolution 72 maie aT hala Resolution Unit Inh Height 2074 pixels Software v756 81 epee ae Date and Time 0000 00 00 00 00 00 Exposure Time 1 30 sec Actual Height 576 um FNumber 2 8 oity aie ODA Exposure Program Normal Delete Descrintion Exif Version 0220 Description Date and Time Original 0000 00 00 00 00 00 Here the image description can be typed a Date and Time Digitized 0000 00 00 00 00 00 Exposure Bias Value 0 0 Evnncire Materinn Mada Dattern T 4 i gt a OK 76 Main Program Window Image Metadata Calibration Calibration Imaging Device 1 Upright Microscope Objective 10 Zoom 1 Change Image Calibration 5 2 Slideshow Option View Start Slideshow runs a full screen presentation of the opened images If only one thumbnail is selected in the Thumbnail Browser all opened images will gradually be displayed If you select more images in the Thumbnail Browser the presentation will contain only these selected images Display of images in the presentation will be gradual in a regular time interval During the presentation you may browse through individual images using the keys Page Up previous image and Page Down next image While you browse through images gradual pro
5. File Name and Type Additional Fle Name Tags Selected Images Tags Flattening Layers Tnformation 1 The red labeled images have the same name and type It is necessary to rename some images Dor Gi Images with yellow exclamation mark already existin the current folder and will be overwritten A Some images are going to be saved as BMP bmp or PNG png type These types do not suppo saving of layers Inserted objects wil be merged with image Information Additional File Name Tags Part Thumbnails of Images for Saving This part contains thumbnails of all images to be saved After the Save As dialog box has been opened all thumbnails of the images to be saved will be selected with an orange frame Thumbnails can be selected deselected in a similar way as the thumbnails in the Thumbnail Browser in the Main Program Window for more information see Thumbnail Browser on page 40 Settings done in the File Name and Type and Additional File Name Tags parts will always be applied to selected images from this list So you can set in bulk the name type and quality for more images at the same time Order of thumbnails on the list can be changed by dragging them with a mouse drag and drop Information about the number of images for saving and the number of selected images are displayed in the bar above the thumbnails If you move the mouse over one of the thumbnails several buttons will be displayed
6. Menu Annotations Menu Annotations contains tools for indication of interesting areas in the image These are the tools available Text Line Arrow Rectangle Circle Ellipse Polygon and Freehand Annotations Imaging A Text Line Z Arrow O Rectangle O Circle Elipse gt Polygon Freehand Menu Imaging Devices Menu Jmaging Devices is used for the choice of an active imaging device Only those devices whose support was chosen during program installation are on the list Menu Modules Menu Modules is used for activation of modules built in modules or optional modules Menu Window Option Restore Default Program Layout is used for setting of the original layout of all parts of the Main Program Window Help Restore Default Program Layout 35 Main Program Window Menu Help Menu Help contains the following items Help opens an electronic user manual You can also use the F1 key Option What Is New displays a file containing a list of all new functions of the current program version Option Licence opens a document with the description of licence conditions Option About displays information about QuickPHOTO DSLR 3 1 program 1 2 Toolbar Individual program functions can also be activated using the following icons from the Toolbar ge Open displays a dialog box for opening of images from the available disc drives for example HDD CD DVD ROM connected networ
7. Time Timestamp Settings lt Back Start Cancel In the Timestamp part you may set the insertion of a timestamp into a video file into each image of the video file will be inserted the information about the date and time of image acquisition This setting will be visible in the video file in such a way that the time will be running in it It is possible to insert Date and Time Appearance of the timestamp may be set after clicking the Timestamp Settings link Timestamp Settings When at least one of the Date and Time checkboxes is checked if you click the Timestamp Settings link a dialog box for setting of the timestamp will open Format using the options in this part it is possible to set the appearance of the timestamp Position this option enables to select into which comer of the video file should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp s text 109 5 Modules FontType Arial m rent Csr ME ci Timestamp Size 50 of Image Width Background Color Transparent Date Format dd mm yyyy X Top4eft Top Right Abc 8 pad a a Bottom eft BottomRight oqVv Preview 12 02 2015 15 32 34 To begin creating a video file click the Start button A dialog box for saving of the file with the avi extension will be displayed Select the desired location and enter the fil
8. 84 Main Program Window Options x 4 General m Default Folders ta fin Mode Image Names amp Saving Continuous Inserting Mode Selected tool remains active until deactivated Sound Language Single Insertion Mode Single object will be inserted upon tool activation Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger Bacup settings i Loadsettings A configuration Wizard ox cana Tab Appearance and Layout On this tab you may select the program s color theme size of the thumbnails in the Thumbnail Browser and the appearance and layout of the Toolbar In the Color Theme part select one of the options and click the Change Theme button In the Thumbnail Browser part it is possible to select the Thumbnails Size by selection from the drop down menu 85 Main Program Window Options 4 General P Default Folders Color Theme Thumbnail Browser Image Names amp Saving Thumbnails Size Sound iors p Black Medium 140 x 105 px Do Not Show This Window Blue Annotations Dark Appearance and Layout Silver Change Theme Miscellaneous 4 Measurements Units Tooke Scale Bar Display Toolbar Buttons with Captions Sizes Colors Other Settings Lock Toolbar and Menus Toolbar and m
9. 115 Troubleshooting FAQ Frequently Asked Questions 116 System Requirements Minimal System Requirements e Single core 2 4 GHz or multi core processor e 1 GB RAM e 1x USB 2 0 3 0 port Supported Operating Systems Microsoft Windows XP SP3 Vista 7 8 8 1 Minimal Monitor Resolution e 1024 x 768 pixels Supported Imaging Devices You can find the list of currently supported imaging devices on the www promicra com website Microsoft Windows Excel Outlook are registered trademarks of Microsoft Corp Canon is a registered trademark of Canon Corp QuickPHOTO is a registered trademark of PROMICRA s r o v 1770 117 System Requirements 118 Index A Acquiring of New Images 42 Acquiring an Image 43 Opening of the Imaging Device s Control Panel 43 Selection of the Imaging Device 43 C Calibration of the Measurements 15 Calibration Manager 16 Creating a New Standard Calibration 17 Opening Information about Calibrations 15 Setting an Active Calibration 27 Configuration Wizard 7 Control Panels of Imaging Devices 95 Canon EOS Digital SLR Cameras 95 Entering License Information 13 I Indication and Annotation of Interesting Areas Draw Arrow 53 Draw Circle 53 Draw Ellipse 53 Draw Freehand 54 Draw Line 52 Draw Polygon 53 Draw Rectangle 53 Program for Insert Text Annotation 54 Initial Configuration of the Program 7 Install
10. Calibrate New Magnification Calibration Calibration Name guide will open on the first page Magnification Selection In the Magnification for Calibration part type in to the Current Objective Magnification field the value of objective magnification of the objective currently in use If you have chosen the No Zoom Magnification Changer option in step 4 while adding a calibration there will be an automatically given value in the Zoom Magnification Changer Magnification part and you will not be able to change this value If you have chosen in step 4 one of the options Zooming Unit or Magnification Changer Unit the Zoom Magnification Changer Magnification field will be empty In this case fill in the value of the currently used zooming unit s magnification or the magnification changer s Continue by clicking the Next button 20 Calibration of the Program for Measurements Calibrate New Magnification Calibration Imaging Device 1 Upright Micr X 1 Magnification Selection Define magnification for calibration Magnification for Calibration Current Objective Magnification 10 Zoom Magnification Changer Magnification af Calibration of the new magnification upright microscope without the magnification changer Calibrate New Magnification Calibration Imaging Device 1 Stereomicros X 1 Magnification Selection Define magnification for calibration Magnifica
11. Default Folders Image Names amp Saving Minimize Imaging Device Control Panel after Image Acquisition Sound Language Do Not Show This Window Display Imaging Device Control Panel after Program Start up Annotations Appearance and Layout Properties of TWAIN Imaging Devices Control Panels Petes Close TWAIN Control Panel after Image Acquisition 4 Measurements Units Scale Bar Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger E Backup Settings GH Load Settings F Configuration wizard Tab Remote Trigger This page is used for setting and verification of the remote trigger s functionality Remote trigger is an optional accessory which can be connected via serial or USB port If you want to use the remote trigger check the Use Remote Trigger option and select the COM port to which it is connected in case of the USB trigger we are talking about the USB Serial Port COM In the Remote Trigger Type part select the type of the used remote trigger Trigger with two buttons for example the two button foot switch is designed for image acquiring and for capturing of video recordings using the optional module for video recording Functionality of the remote trigger can be verified by pressing its button s If the small square s change color to green after you press the button s and they turn back to black after you release the button s the remote trigger is set correctly and it is ready t
12. in the QuickPHOTO DSLR 3 1 program with the layers kept as they are QuickPHOTO DSLR 3 1 program uses LZW compression algorithm for saving of TIFF files Information about PNG File Type PNG file type Portable Network Graphics suffix png also uses the lossless compression for saving image data PNG type produces unlike the TIFF type 60 Main Program Window significantly smaller files but it does not allow in the QuickPHOTO DSLR 3 1 program to save images with the layers kept as they are PNG type is especially suitable for publishing of smaller images on the internet Information about BMP File Type BMP file type Windows Bitmap suffix bmp represents in terms of how to save image data the most primitive way of saving images to a file BMP type saves image information to a file without compression and because of that the result is a very large file BMP type does not allow in the QuickPHOTO DSLR 3 1 program saving of images with the layers kept as they are Note QuickPHOTO DSLR 3 1 program saves images to files in the 24bit RGB mode and sets the DPI parameter to value 300 The images can also be saved as 8bit grey scale TIFF files Image Saving There are several options for saving images in the File menu These are the following options Save Save As Save All Save All As You can also use key shortcuts to activate individual options save Ctri S A Save As Shift Ctrl S Save all Ct
13. left mouse button on such a point of the image which will define the reference black color for the whole image Black balance will take place in the whole image based on the defined reference value This function is designed for fluorescence applications 48 Main Program Window Adjust Brightness Contrast Gamma Adjust Colors Options Image Adjust Brightness Contrast Gamma and Image Adjust Colors or corresponding icons and open an Image Adjustment dialog box with tabs Brightness Contrast Gamma and Red Green Blue In the top part of both tabs there are sliders for individual options and in the bottom part there are previews of the image before any changes and after changes have been made Buttons and between both previews are there for the change of display size of both previews After previews have been zoomed in it is possible to by dragging the mouse while holding the left mouse button move both previews Using sliders of individual functions it is possible to adjust the desired image parameters Changes will immediately be visible in the right hand side preview Default Values button in the bottom part of the window will bring all settings in this dialog box to the original state Changes made can be applied to the whole image by clicking the OK button Image Adjustment x Brightness Contrast Gamma i Red Green alue Be TIN Tiers a Contrast y 63 Y Gamma y 0 49 Preview Aft
14. 4 images in the Thumbnail Browser and you activate the Print function only the first four images of the selection will be printed Print Window Print window consists of several parts e Preview e Printer tab e Page Layout tab e buttons in the bottom part 68 Main Program Window x Printer Name HP Color LaserJet 2605 UPD PS s Status Ready Properties Type HP Universal Printing PS Orientation Color Mode Portrait 3 Color Landscape Grey Scale Copies Units Copies 1 cm inches Margins Top 0 43 cm Left 046 cm Right 0 47 gt cm Bottom 0 43 cm Minimal Margins o Paper Size AS Printer DPI 600 WS Defaut Settings Print Cancel Apply Part Preview In the right part of the Print window is displayed the page Preview as it will be printed out Settings made are continuously updated in the Preview field It is then possible to make required print settings interactively Under the Preview there are displayed information about the currently selected paper format and the set print quality units DPI Dots Per Inch Printer Tab In the Printer part choose the desired printer from the Name drop down menu which contains the list of available printers Properties button opens a dialog box with advanced settings for the chosen printer 69 Main Program Window Orientation par
15. Ctrl T White Balance Defined by Point Fl Help F4 Open imaging device control panel F11 Options of the program Alt S Start Slideshow 113 List of Key Shortcuts Key Shortcut Action Ctrl Alt C Calibration Manager Ctrl F Display in Full Screen Mode Alt X Exit program Ctrl A Select all thumbnails in the Thumbnail Browser Ctrl selection with a mouse Selecting discontinuous selection of thumbnails in the Thumbnail Browser Shift selection with a mouse Selecting of more inserted object or a continuous selection of thumbnails in the Thumbnail Browser Shift during object insertion Inserting a horizontal or vertical line Page Up Display the previous image in the presentation mode Page Down Display the following image in the presentation mode Space Bar Continue the slideshow Key shortcuts used in imaging device control panels Key Shortcut Action Ctrl Enter Capture image Ctrl F Display in Full Screen Mode on the numeric keyboard Extension of the exposure time while using the manual exposure mode Page Up Extension of the exposure time while using the manual exposure mode on the numeric keyboard Shortening of the exposure time while using the manual exposure mode Page Down Shortening of the exposure time while using the manual exposure mode 114 Troubleshooting FAQ Frequently Asked Questio
16. Do Not Show This Window the program during image opening anes y Display confirmation dialog box for fattening of layers when saving an image as JPG or TIFF type with the Appearance and Layout Merge Objects with an Image option activated If unchecked layers will be automatically flattened a x Display confirmation dialog box for automatic flattening of layers when saving an image as PNG or BMP type If 4 Measurements Y unchecked layers will be automatically flattened Units y Display confirmation dialog box about deleting all inserted objects when resizing an image Scale Bar Tf unchecked all inserted objects will be automatically deleted Sizes Colors y Display confirmation dialog box when deleting all inserted objects Other Settings wi tay all inserted objects will be automatically deleted lt Herdwere y confirmation dalog box about discarding al changes when restoring orignal mage Imaging Devices Y Tf unchecked all changes will be automatically discarded Remote Trigger p Gel Badupsettings G Loadsettings Configuration Wizard ox Cancel Tab Annotations On this tab you may set the mode of inserting annotations and drawings If the Continuous Inserting Mode option is active the selected tool for annotating or drawings will be active until its manual deactivation If you activate the Single Insertion Mode option selected tool will be after inserting of one object automatically deactivated
17. E05 Digtal SLR Camere Size ratios of individual parts of the Main Program Window described above can be changed This change can be done in the following way shift your mouse over the border separating individual parts Mouse cursor will change to 4 Click and hold the left mouse button and define the new size by moving the mouse Then release the button 30 Main Program Window Note The default program layout can be restored using the Restore Default Program Layout option in the Window menu 1 1 Menu Bar Menu File File menu contains the following functions Open opens images in the Main Program Window In the opened Open dialog box you may select one or more images and load them at once Option Open Recent offers up to ten lately used images for opening Options Save and Save All save images selected in the Thumbnail Browser respectively all opened images If the images being saved have already been saved before they will be saved again into their original locations If we are speaking about newly acquired images a dialog box Save As will be displayed and you may choose the location name and type of the files being saved Options Save As and Save All As will open the Save As dialog box which enables you to choose the location name and type of the files being saved For more information about image saving see Image Saving on page 58 Options Close Selected Images and Close All Images will cl
18. In such Sound case untranslated parts will display in English Language e tina Do Not Show This Window CEE o Annotations English Appearance and Layout Espa ol Miscellaneous Magyar 4 Measurements Slovendina Units Pycam Scale Bar Yrpa hcoka Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger H Badup Settings Load Settings Configuration Wizard OK Cancel Tab Do Not Show This Window Some dialog boxes in the QuickPHOTO DSLR 3 1 program contain the Do Not Show This Window option If you check this option in some of the dialog boxes this dialog will not be displayed during the next activation of the corresponding function and the default action will be automatically done The corresponding option on this tab will be unchecked This setting will be remembered for the next run of the QuickPHOTO DSLR 3 1 program If you want to restore displaying of some of the dialog boxes check its corresponding option on this tab 83 Main Program Window Options x 4 General Default Folders Do Not Show This Window Image Names amp Saving y Display dialog box for change of active calibration upon a change of imaging device Sound iF unchecked active calibration will be retained when changing imaging device Language re 7 Display information about using calibration contained in the image when the required calibration is not found in
19. Page No Aspect Ratio this option uses the whole page area for printing Original aspect ratio will not be preserved Custom options in this part enable to set custom image print size in length units If the Constrain Proportions option is checked and you will type in the first dimension the second dimension will be calculated automatically in the original aspect ratio of the image This option is available only for print of one image on a page Scale options in this part enable to set custom image print size in percents compared to the original size If the Constrain Proportions option is checked and you will type in the first dimension the second dimension will be automatically calculated in the original aspect ratio of the image This option is available only for print of one image on a page In the Image Alignment part you can set the alignment of images on the page by clicking one of the 9 buttons Part Image Information sets print of additional information about the image Along with the image it is possible to print after checking the corresponding options Image Name Date and Time of Image Origin and Description for more information see Image Information on page 75 Buttons in the Position part determine the position towards the image where additional information will be located Information is for better orientation highlighted with a green color in the Preview This highlight will not show in the final print In
20. Panel opens a control panel of the active imaging device device chosen in the Jmaging Devices menu An arrow in the right part of the icon is used for a quick choice of the imaging device F4 Time Lapse Video activates a built in Time Lapse Video module designed for creating of video files from the acquired time lapse images Timer activates a built in Timer module designed for an automatic acquiring of images in an adjustable time interval Best Fit Zoom displays the current image in such a size so it fits into the Display Window Zoom In increases the size of display of the current image by one step 37 Main Program Window p 4 1 75 Zoom Out decreases the size of display of the current image by one step Zoom Actual Size 100 displays the current image in its real size 100 Display Size this field indicates current displayed image size It enables you to pick your desired size from the drop down menu of various values or you can using the keyboard type in a new value Crop after clicking on this icon you may select an area of the image which should be preserved Remaining part of the image will be removed Rotate Clockwise by 90 rotates the image to the right Rotate Counter Clockwise by 90 rotates the image to he left Flip flips the image around the horizontal axis Mirror mirrors the image around the vertical axis Resize opens a dialog box used for a change of the
21. Quality 95 Part Location In this part it is possible to select a folder into which all images displayed in the Thumbnails of Images for Saving part will actually be saved Control in this part is similar to the standard Windows dialog box for saving files 65 Main Program Window Location J Samples X ojele ee v a E samplet jpg Desktop 88 sample4 png Documents iy Computer Network Part File Name and Type In this part it is possible to choose the names and types of images selected in the Thumbnails of Images for Saving part If you have selected more than one thumbnail and you type in a new name to the File Name field the name of all selected images will be changed at the same time and the Numbering function will also be activated This function will ensure distinction of individual images Changes will immediately appear in Thumbnails of Images for Saving part If you want to restore the original names of the selected images click the Restore button Save as Type drop down menu enables to select the type format of the images selected in the Thumbnails of Images for Saving part If you leave the default option Original Type active images will be saved in the same format in which they were opened acquired in the program If you choose one of the available types from this menu the selected type will be set to the images
22. Source tab select the source of images from which the resulting video file is supposed to be created There are three options available Process Images Selected in the Thumbnail Browser select this option if the video file is going to be created from a smaller number of images which are opened in the Main Program Window of QuickPHOTO DSLR 3 1 program Images which were in the time of running of the Time Lapse Video module selected in the Thumbnail Browser will be processed Select Saved Files for Processing Up to 2000 Files select this option if the images intended for processing are saved on a hard drive After clicking the Next button a dialog box for files selection will open Select all files from which you want to create the video file Maximum number of files is affected by the length of their names and it may reach the maximum value of 2000 In case of long file names this number may be smaller Select the Whole Folder for Processing select this option if the images intended for processing are saved in a folder which does not contain any other images Processed will be all images from the selected folder therefore be careful not to forget any unwanted images among the images intended for processing This option enables processing of virtually any number of images Click the Next button 107 Modules 4 J Time Lapse Video x Selection of Image Source This wizard will help you create video file from ti
23. Then by clicking into the image and dragging the mouse while holding the left mouse button or by clicking the left mouse button into the starting point and then into the endpoint an arrow may be drawn The arrowhead will be placed to the endpoint Precisely horizontal or vertical arrow may be drawn if you hold the Shift key while drawing it Draw Rectangle Tool Draw Rectangle is activated by the Annotations Rectangle option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw a rectangle Draw Circle Tool Draw Circle is activated by the Annotations Circle option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw a circle Draw Ellipse Tool Draw Ellipse is activated by the Annotations Ellipse option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw an ellipse It is possible to rotate the ellipse by dragging the green grasp point displayed inside the ellipse Draw Polygon Tool Draw Polygon is activated by the Annotations Polygon option or by clicking the icon Then by clicking the left mouse button into the selected area in the image insert the first polygon s vertex Repeated mouse clicks of the left mouse button will insert other vertexes of the polygon Precisely horizontal or ver
24. bar Created settings can be saved into one of the four available profiles with the option to choose your own name If you activate the option Insert Scale Bar into Image Automatically after Magnification Assignment a scale bar will be automatically inserted into images after you select magnification in Objective 74 Main Program Window and Zoom menus in the Toolbar which was used for their acquisition For more information about the scale bar settings see Tab Scale Bar on page 88 Further in the context menu there are four preset scale bar profiles These items correspond with the user definable profiles described in the previous paragraph Activation of the selected scale bar profile can be done by selecting a profile name with a mouse Scale bar modifications will be reflected in all images 5 Other Functions 5 1 Image Information Information about the current image can be displayed by activating the Image Image Information option by clicking the icon in the Toolbar or by clicking the Image Information Li button in the controls of the corresponding thumbnail in the Thumbnail Browser Image Information Image Name dialog box which can have up to three tabs Image Metadata and Calibration will open Image Tab This tab gives the following information e Path displays information about the image s location e Name corresponds with the image s name e Size shows the size of the saved
25. continue When automatic image saving is set only the Build on Previous Numbering option will be available At the same time the How to Solve Name Conflicts Automatic Saving part will be made available This option defines the program s behavior in cases a conflict appears during automatic image saving in the names of the newly saved and previously saved images There are two options available Increase the Image Number Automatically newly saved image will be labeled with a one step higher number than the number of the image with the highest number in the folder Further on the program will for newly acquired images pick up where this numbering has ended Overwrite Existing Images option will cause automatic overwriting of already saved images by the newly acquired ones without displaying any warning For automatic saving of new images is also available a Flattering Layers during Automatic Image Saving part If you activate the Keep Layers option acquired images being saved as JPEG or TIFF type will be saved with layers kept If the Automatically Flatten Layers options is active images of all types will be saved with the flattened layers 81 Main Program Window For images of the PNG and BMP type will the layers always be automatically flattened during automatic image saving In the Default JPEG Quality part it is possible to preset the default quality for saving of images of the JPEG type This setting will be used for the aut
26. default state Active mode for automatic image saving will be indicated by the icon in the status bar in the right bottom corner of the program If you move the mouse over this icon information about the folder where images are being saved will be displayed In the Type of New Images part it is possible to select the type format which shall have the newly acquired images Original Type option preserves the image type which is provided by the active imaging device If you select one of the JPEG BMP TIFF or PNG types from the list all newly acquired images will be converted to the selected type This option is valid even for automatic image saving If automatic image saving is set you may set or change the folder into which the acquired images will be saved in the Folder for Automatic Image Saving part If the option Program Only is active in the Store Images after Acquisition in part it is possible to in the Numbering of Acquired Images part select the Always Begin Numbering from 00001 after Program Start up mode When this mode is active numbering of acquired images will begin with 00001 after each start of the QuickPHOTO DSLR 3 1 program If you select the Build on Previous Numbering option numbering of acquired images will start from the last used number QuickPHOTO DSLR 3 1 program will remember the last number of the acquired image when being terminated and when you run the program again numbering of the acquired images will
27. displayed after one of these functions File Save or File Save All has been activated while re saving the JPEG or TIFF type images into their original locations Saving Parameters x In this window parameters for saving of all images of the JPEG and TIFF types which will be saved to their original locations can be adjusted BMP and PNG images will be saved automatically JPEG and TIFF Images to Be Saved sample 1 jpg Flattening Layers Common Option for All Images in the List Keep Inserted Objects in a Separate Layer Merge Objects with an Image Flatten Layers JPEG Quality Quality 95 gt High 1 a0 i Small File Large File File Size Not Calculated Calculate P 62 Main Program Window In the top part of the window there is a list JPEG and TIFF Images to Be Saved which contains names of images which will be involved in the settings The same settings will be used for all images on this list In the middle part of the window there is a Flattening Layers option available for more information see Information about Work with Layers on page 58 If there is at least one JPEG type image in the list there is a JPEG Quality section in the bottom part of the window Controls in this section enable setting of quality for the JPEG type images This option has no influence on other types of images Calculate and Preview buttons are available only if ther
28. displayed in the Main Program Window To select the folder click the button 104 Modules and select the desired location Enter the file name of the sequence being captured into the File Name field Individual images will be automatically differentiated using the five digit serial number attached after the selected name In the bottom part of the dialog box there are displayed information about the process of time lapse image capturing In the Time until Next Capturing part the time remaining until next image capturing will always be displayed Further you will have information about how many images have already been captured in the current sequence in the Number of Captured Images part and how many images remain to be captured in the Number of Remaining Images part Timer x Capturing Number of Images Number 10 ime Interval Interval between Images Hours Minutes Seconds 0 Pa o a S Store Images in the Main Program Window in Folder C Sequences File Name Timer Time until Next Capturing 00 00 00 Number of Captured Images 0 Number of Remaining Images bes 105 Modules 1 2 Recommendations for the Time Lapse Image Capturing To achieve optimal results during time lapse image capturing we recommend the following Use manual exposure mode of the imaging device for i
29. image Click on the text annotation with the left mouse button and move the text by dragging the mouse while holding the left mouse button to the desired area 55 Main Program Window To edit an already inserted text annotation double click on it with the left mouse button A Text Annotation dialog box will open again and you will be able to edit the text as you wish Selection of the Inserted Objects for Editing If you want to edit objects that have already been inserted into the image it is necessary to select those objects first Proceed according to the following instructions Activate the Select tool and by clicking the contour line of the desired object select it Selected object will be highlighted with a green dash line and on its perimeter green grasp points will be activated except for an object created with the Draw Freehand tool Object selected this way is ready for editing To select more objects at the same time press and hold the Shift key and select all objects which you want to edit with the left mouse button To cancel the object selection click the left mouse button anywhere outside the objects or click the right mouse button Selection of objects will be canceled Note Select tool is automatically activated every time no other tool is active Change of Line Color and Thickness of the Drawn Objects In order to edit inserted objects select them first for more information
30. image size Adjust Brightness Contrast Gamma opens a dialog box for brightness contrast and gamma correction adjustment Adjust Colors opens a dialog box for color balance adjustment It is possible to independently adjust the red green and blue channel Adjust Histogram opens a dialog box for the histogram adjustment 38 Main Program Window Object Line Color Thickness is used for a change of color and thickness of the lines of inserted objects Left part of the icon enables a change of color right part with an arrow enables change of the line s thickness N Select is used for selection of inserted objects x Delete Selected Objects deletes objects selected with the Select tool Del _ Delete All Objects deletes all objects inserted in the image A Insert Text Annotation activates a dialog box for inserting of a text annotation Draw Line activates a tool for drawing of a line Draw Arrow activates a tool for drawing of an arrow co Draw Rectangle activates a tool for drawing of a rectangle Draw Circle activates a tool for drawing of a circle lt gt Draw Ellipse activates a tool for drawing of an ellipse lt gt Draw Polygon activates a tool for drawing of a polygon Draw Freehand activates a tool for freehand drawing 39 Main Program Window 1 3 Thumbnail Browser Thumbnails of the opened images are displayed in the Thumbnail Browser together with their names and si
31. images will be acquired The available sizes will be affected by a potential use of a Crop Borders function from the Settings tab 96 Control Panels of Imaging Devices Exposure Exposure this part contains control elements for setting the camera s Sensitivity ISO Mode Auto Exposure Exposure Correction exposure The Mode indicator displays the current camera s mode set by its mode dial To acquire images from a microscope set the mode dial to Av automatic mode or M manual mode The 0 Auto White Balance ato ETEA Defaut Exposure Region E Center Zoom In Exposure Correction drop down menu enables you to lighten or darken the images when the automatic exposure mode is set The Sensitivity ISO drop down menu sets the sensitivity of the sensor The Exposure Time drop down menu is enabled only when the manual exposure mode is set You can set the required exposure time by this menu White Balance the automatic white balance can be done in two ways If you click the Auto button the white balance will be performed automatically by the camera Use this function in case you want the digital image to have the same color balancing as the image visible in the eyepieces If you click the One Point button click the left mouse button to the desired point of the live view which will define the reference white color The white balancing will b
32. of both previews After previews have been zoomed in it is possible to by dragging the mouse while holding the left mouse button move both previews Default Values button in the bottom part of the window will bring all settings in this dialog box to the original state Changes made can be applied to the whole image by clicking the OK button 3 3 Indication and Annotation of Interesting Areas Program QuickPHOTO DSLR 3 1 is equipped with functions for indication of interesting areas in the image using drawings and text labels You may use the following tools Text Annotation Draw Line Draw Arrow Draw Rectangle Draw Circle Draw Ellipse Draw Polygon Draw Freehand The use of individual tools is described in the following paragraphs 51 Main Program Window Note The use of the above mentioned tools can be canceled anytime by the right mouse button click or by the Esc key Annotations Insertion Mode Program QuickPHOTO DSLR 3 1 offers two modes of annotations inserting Make your selection in Options of the program in section General at the Annotations tab Edit gt Options General Annotations in the part Annotations Insertion Mode There are two options available Continuous Inserting Mode if this option is active the selected tool will remain active for the current image until it is deactivated This way you may repeat inserting of the same type of object into the image without the nee
33. on the right side next to the particular thumbnail These buttons call for functions for a specific image Image preview in the full size can be displayed by clicking the Preview button 64 Main Program Window for JPEG image types the quality of the preview will be influenced by the set JPEG Quality parameter information about the image can be displayed using the Image Information 4 button This function is useful for example if you want to add a description to the image before you save it Images can be removed from the saving list by clicking the Close x button thumbnail will disappear from the list EERE ee sample2 jpg Pe 2048 x 1536 JPEG Quality 95 Two indicators may be displayed in the thumbnail area according to the current situation If there is an already existing image with the same name and type in the folder chosen in the Location part an indicator will be displayed by the thumbnail Unless you change the name or type of the image the already existing image in the folder will be overwritten If the indicator is displayed by some of the thumbnails it means that there has been a collision of the names as well as the types of the images on the list In such a case saving of the images cannot take place unless you change the name or type of these images Legend for the displayed indicators will be displayed in the nformation part l ela x sample 1 jpg 6464 x 4864 JPEG
34. selected in the Thumbnails of Images for Saving part If you choose the JPEG type indicator of the set JPEG quality will also be displayed by the corresponding thumbnails for example JPEG Quality 95 and at the same time JPEG Quality link will be made available right from the Save as Type drop down menu This link will open a window in which it will be possible to set the desired quality of the JPEG type images File Name and Type File Name sample 1 Restore Save as Type Original Type ki JPEG Quality 66 Main Program Window Part Additional File Name Tags In the part Additional File Name Tags there are three buttons Button Date will add the current date to the image names selected in the Thumbnails of Images for Saving part Button _ Time will add the current time to the image names selected in the Thumbnails of Images for Saving part Button _Numbering adds a serial number to the image names selected in the Thumbnails of Images for Saving part Images will always be numbered from the number 00001 This function is useful for example in case you want to save several images from the same series with the same name and you want to distinguish them with a serial number The use of particular buttons can be freely combined Part Flattening Layers In this part of the window there is the Flattening Layers option available for more information see Information about Work with Layers on page 58 Setting of this opti
35. the Navigator window there is a framed area of the image which is visible in the Display Window at the current display size Display of the current image can be enlarged or reduced with the mouse wheel slider or with the or buttons under the Navigator window It is possible to move the frame by clicking the left mouse button inside the frame and by moving the mouse while holding the left button By moving with the frame you will achieve the desired display of a particular image area in the Display Window 41 Main Program Window i i 1 5 Display Window Display Window is designed for work with images 1 6 Status Bar Status bar placed in the bottom part of the Main Program Window displays the following information e size of the current image in pixels and if the current image has an assigned calibration real size of the image in mm or ym e currently selected imaging device if you have set the automatic saving of images to a folder there will be an icon in the right corner of the status bar If you move the mouse over this icon a hint will be displayed with currently set folder for automatic saving of images 2 Acquiring of New Images To acquire new images proceed according to the following steps 1 Select the imaging device 2 Open the control panel of the imaging device 3 Perform the image acquisition 42 Main Program Window 2 1 Selection of the Imaging Devi
36. the displayed image mark the highest possible number of the stage micrometer segments with a line segment by dragging the mouse while holding the left button or by clicking the left mouse button on the beginning and end point Insertion of a precisely horizontal or vertical line segment can be done by holding the Shift key while inserting the line segment To achieve the highest possible accuracy it is good to enlarge the image using icons in the upper left part of the dialog box or by using the mouse wheel before inserting the calibration line segment Lead the line segment from the left edge of the initial guideline to the left edge of the ending guideline of the calibration scale Incorrect insertion of the line segment can be corrected by inserting a new calibration line segment Calculate the real length of the marked part of the calibration scale according to the number of marked segments and type the value in the Actual Length of the Line Segment field according to the measuring units chosen in the Units section To save the calibration value click the Finish button Value for new magnification will be added in the Table of Calibration Values and Graph of Calibration Values 22 Calibration of the Program for Measurements 3 Calibration Step 1 Drag a line segment to mark the maximum number of stage micrometer divisions Drag the line segment from the left border of the first division line to the left border of the final divisio
37. the selected string for each new image Current date can also be attached next to the serial number when acquiring an image using the _Date button or possibly current time using the _ Time button Preview of the set name is displayed in the Example field Default button renews the default settings of the name Images from Time Lapse Capturing part contains identical elements for setting of names of the time lapse images such as the Acquired Images part Settings will take effect for images acquired by the Timer module directly into the program 79 Main Program Window Options 4 General Default Folders eftenImeoes Image Names amp Saving Acquired Images P Date Tme Defaut Sound 3 Language Example P00001 jpg i Do Not Show This Window Images from Time lapse Capturing T Date time Defaut Annotations z Appearance and Layout Example T00001 jpg Miscellaneous Store Images after Acquisition in Type of New Images 4 Measurements Units Program Only Type JPEG jpg Scale Bar Sizes Colors Folder for Automatic Image Saving Other Settings 5 E 4 Hardware Imaging Devices ing of Remote Trigger How to Solve Name Conflicts Automatic Saving Always Begin Numbering from 00001 after Program Start up Increase the Image ber Auto Build on Previous Numbering 00001 Flattening Layers during Automatic Image Saving
38. using filters sharpen blur negative grey scale median filter black and white balance adding text annotations to an image labeling with drawings tools line arrow rectangle circle ellipse polygon freehand drawing QuickPHOTO DSLR 3 1 program is also equipped with the function for inserting a calibrated scale bar with the option to define its look and position All inserted objects drawings annotations scale bar are displayed in a separate layer above the image and it is possible to modify change of the line s color and thickness change of size change of position delete or hide them at any time It is also possible to delete a whole layer with inserted objects or it is possible to merge this layer with the image 1 Description of the Graphical User Interface Main Program Window is divided into several parts 29 Main Program Window e Menu Bar contains options with individual functions Toolbar contains tool icons Thumbnail Browser displays all opened images e Navigator is used for orientation in the enlarged image e Display Window is used for image editing e Status Bar contains information about the current program status Display Window Menu Bar gt t imase vew Weasuwements Annotations magng Desces Window Hep SS Ol BB t oweos zonis ajere 3 Toolbar sone ips Thumbnail Browser Navigator Status Bar p gt 22 88 tine 5 250m T conan
39. y it will apply also to the acquired images Flip Rotate by 180 Cross displays hides the central cross The displayed cross will not be Gross visible in the acquired images Grey Scale turns on off the grey scale mode Grey Scale Timestamp Tab This feature enables automatic insertion of date and time of acquisition the so called timestamp into the acquired images The timestamp will after acquisition of the image become a solid part of the image and it is not possible to remove it from the image other way than by cropping it 99 Control Panels of Imaging Devices Timestamp Timestamp Disabled Enabled Insert Date Time V Seconds Orientation Abc Timestamp Timestamp this option turns the Timestamp function on off Disabled Enabled V Date V Time Seconds Insert by checking of individual options it is possible to define which components of the timestamp will be inserted into the acquired images Orientation Orientation this field serves as an indicator of the set orientation Abc of the timestamp If you double click into this field a Timestamp Settings dialog box will open Options Options if you click this button a dialog box for setting of timestamp parameters will open Timestamp Options If you click the Options button or if you double click into the Orientation field a
40. your mouse while holding the left button over the area which shall be preserved the rest of the image will be cropped In the left upper corner of the selected area will appear a field showing its size Size is displayed in pixels and if there has been a calibration n also in length units HI T Clicking on the field displaying size will switch it to a mode in which it is possible to type in the values and to define precisely the desired size of the chosen area Edit Selection Width 1422 7 pixels Height 1426 J Right from the field displaying size values is a lock icon If an open lock is displayed default state it is possible to enter both dimensions of the area independently After clicking on this icon a closed lock will be displayed This way the program will remember lock the ratio of the area s dimensions Then after you have entered one dimension the second one will automatically be calculated in the locked ratio If the image has an assigned calibration it is possible to after selection of units from the drop down menu on the right from the lock icon define the area dimensions in units of length New size can be confirmed by the OK button Recalculation of the area size will take place according to the entered values Field displaying the size can be for better clarity hidden using the button To display the area size values again use the button 45 Main Program W
41. In the opened Open dialog box you may select one or more images and open them all at the same time Another possibility for opening of images is to drag them with a mouse into the Display Window drag and drop for example from the Windows Explorer or another file manager Thumbnails of the opened images will appear in the Thumbnail Browser together with their names types and sizes Menu File Open Recent offers up to ten last used images on a list for opening amp Open Ctri 0 Open Recent 3 2 Image Editing Note All editing is always done to the currently displayed image in the Display Window Options for image editing may be activated from the mage menu Some of these options may also be activated using the icons in the Toolbar Menu Image contains the following options for image editing Crop e Rotate Clockwise by 90 and Rotate Counter Clockwise by 90 e Flip vertically and Mirror horizontally e Resize e Auto White Balance White Balance Defined by Point e Filters Sharpen Blur Median Filter Negative Grey Scale Black Balance Defined by Point Adjust Brightness Contrast Gama Adjust Colors Adjust Histogram Detailed description of individual functions is in the following paragraphs 44 Main Program Window Cropping Image Function Crop is used for removal of undesirable image areas Activate Crop function from the Image Crop menu or by clicking the 4 icon Drag
42. Magnifications Calibration Calibration Name the actual process of calibration will be done In the Table of Calibration Values part you will find columns named Objective Position and Zoom Magnification Changer Magnification If you have chosen the No Zoom Magnification Changer option in step 4 while adding a calibration there will automatically be given the value 1 in the last column If you have chosen in step 4 one of the options Zooming Unit or Magnification Changer Unit this column will be empty and individual values of the zoom magnification changer will be gradually added to the table during the process of calibration of the particular magnifications Column Position is not used in this 19 Calibration of the Program for Measurements 2 case In the right part of the dialog box there is only one active button in this moment Calibrate New Magnification There is a Graph of Calibration Values in the bottom part of the dialog box which is used for checking of the correctness of the calibration procedure To continue click the Calibrate New Magnification button Table of Magnifications Calibration Imaging Device 1 Upright Microscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification Objective Position 1 Graph of Calibration Values 1Calibration Value 1mm Objective Magnification o
43. QuickPHOTO DSLR 3 1 User Guide QuickPHOTO DSLR 3 1 QuickPHOTO DSLR 3 1 Copyright 2015 PROMICRA s r o All rights reserved QuickPHOTO DSLR 3 1 program is not designed for diagnosis and determining of medical procedures Table of Contents nst llao a a hie t a a a e deena 304 Suan ae tae aioe ais 5 1 Installation of an Imaging Device ssnsseseseesresesersessersrsrerreseeseerrsserersse 5 1 1 Installation of Canon EOS Digital SLR Cameras s seeseeees eere ereeee 5 2 Installation of the QuickPHOTO DSLR 3 1 Program eeeseseeeesseereseerreseese 6 Initial Configuration of the Program s ssessessesresesresrssererssererssesrerersresrsseree gt i 12 Configuration Wizard enee ree e a e a T E A R EE E 7 Entering License Information s essssessrssersrsserresrrsersrserersserrrssesresersrerrsserer gt 13 Calibration of the Program for Measurements cceeeeeeeeceneeeeeeeeeeeeceaeeeees 15 1 Opening Information about Calibrations esseeeeeeeeseesseesrerereesreesreerreesee 15 2 Calibtation Manager oeiee e e Santen a ree ge bot Sac Saka ped aes 16 3 Creating a New Calibration cccceecceeceeeceeeceeeceeeea cece ees eeaeeeneeeneeenaes 17 4 Setting an Active Calibration cccccecc cece eeeeeceeeceeeeeueeeeeeneeeeeeneseaeeees 27 Main Program Window cceecceeceeeceeeceeeceeeeeeeceeeee cece ees eeaeeeaeeeaeeeeneeeneeeeeges 29 1 Description o
44. a Separate Layer 2 Merge Objects with an Image Flatten Layers Both saving options have their specifics 1 Keep Inserted Objects in a Separate Layer It is possible to keep the layers separate only when saving images as the JPEG or TIFF type Images of the BMP and PNG type do not support saving of layers If you save the image as a JPEG or TIFF type with the layers kept all its functions will be available next time you open it in the QuickPHOTO DSLR 3 1 program Which means that the image will have an assigned calibration it will be possible to edit all inserted objects and description of the image will be maintained Important is the fact that after opening of an image saved in such a way in other programs for work with images the layer containing inserted objects will not be visible only the image without inserted objects will be displayed 2 Merge Objects with an Image Flatten Layers If you need the inserted objects in the image to be visible also in other programs for work with images for example if you need to insert the image to a text document or a presentation it is necessary to save the image with flattened layers In such case the layer above the image containing inserted objects will be merged with the image When 58 Main Program Window you flatten layers all additional information image calibration description of the image will be removed from the image When you open an image saved in such a wa
45. agnification which Was Used for Acquiring of the Image Before you begin measuring first you must assign the corresponding microscope magnification which was used when acquiring the image intended for measuring Proceed as follows 1 Display the desired image in the Display Window 2 Inthe Toolbar choose from the Objective or eventually Zoom menu the corresponding magnification In the list you can only choose from those values for which calibration was created If your microscope is not equipped with a magnification changer or a zooming unit there will be after you choose an objective in the Zoom menu automatically set the value 1 After this step functions for measuring will be activated Objective 10 Zoom i p Mode of Assigning Magnifications to Images If the same microscope magnification is being used frequently manual assigning of the used magnification to each new image might be uncomfortable For such cases offers the QuickPHOTO DSLR 3 1 program in the Options dialog box in section Measurements on the Other Setting tab Edit Options Measurements Other Setting the option Mode of Assigning Magnifications to Images This option offers two possibilities Manual Magnification Assignment to Each Image if this option is active default option it is necessary to manually set magnification from the Objective or eventually Zoom menu to each image intended for measurements Automatically Assign th
46. alibration Manager Ctrl Alt C option 2 After clicking the Add Calibration button a guide Add Calibration opens where in the first step you choose the name of the calibration being created The name of a calibration consists of two parts from the name of the imaging device and from the microscope type At first choose the type of the used imaging device from the 17 Calibration of the Program for Measurements J drop down list in the Jmaging Device part or click Custom option and enter your own name of the imaging device we use the name Jmaging Device 1 or Imaging Device 2 as general examples in following chapters In the Microscope part enter the type of the used microscope we use upright microscope or stereomicroscope as general examples in following chapters Continue by clicking the Next button Calibration Manager x Calibration Name Objective Encoder Zoom Encoder Lock Calibrations E Backup Calibrations Load Calibrations Close Add Calibration x Calibration Name Select imaging device and microscope type to define calibration name Imaging Device Select from the List z Custom max 30 chars Imaging Device 1 Microscope Microscope Type max 30 chars Upright Microscope Next page of the guide Zoom Magnification Changer Selection asks about the microscope equipment zooming unit or the magnification changer If the mi
47. ation 5 Canon EOS Digital SLR Cameras 5 QuickPHOTO DSLR 3 1 Program 6 L List of Key Shortcuts 113 Main Program Window 29 Description of the Graphical User Interface 29 Display Window 42 Menu Bar 31 Navigator 41 Status Bar 42 Thumbnail Browser 40 Toolbar 36 Measurements 72 Preparation for Measuring 73 Scale Bar 74 Menu Bar Menu Annotations 35 Menu Edit 32 Menu File 31 Menu Help 36 Menu Image 33 Menu Imaging Devices 35 Menu Measurements 34 Menu Modules 35 Menu View 34 Menu Window 35 Modules 103 Time Lapse Video Built in Module 106 119 Index Timer Built in Module for Time Lapse Image Capturing 103 0 Options 78 Section General 78 Section Hardware 92 Section Measurements 88 Other Functions 75 Display in Full Screen Mode 77 Image Information 75 Slideshow 77 S System Requirements 117 T Troubleshooting FAQ Canon EOS DSLR Cameras 115 Time Lapse Video 115 W Work with Images 43 Image Editing 44 Image Opening 44 Image Printing 68 Image Saving 58 Indication and Annotation of Interesting Areas 51 120
48. be pasted into the QuickPHOTO DSLR 3 1 program as a new image This way you may paste an image from a different program or an image or its chosen area from the QuickPHOTO DSLR 3 1 program When you paste an image from the QuickPHOTO DSLR 3 1 program the new image will be pasted with merged layers To activate the paste function use the Edit Paste option click the icon or use the Ctrl V key shortcut 46 Main Program Window Flipping and Rotating the Image It is possible to rotate the image by 90 clockwise or counter clockwise using the options Image Rotate Clockwise by 90 and Image Rotate Counter Clockwise by 90 or using the i and icons For vertical flipping or horizontal image mirroring use one of the options Image Flip or Image Mirror or one of the and icons Resizing Image To change the image size activate the Image Resize option or click the icon A Resize dialog box will open Type in one new image dimension second dimension will be calculated automatically in the original aspect ratio Ratio of the dimensions is always kept the same to maintain accuracy of measurements Confirm the new image size by clicking the OK button Resize x Current Size Width 2447 pixels Height 1919 pixels New Size Width 800 pixels Height 627 gt pixels Aspect ratio will be maintained to preserve measurement accuracy OK Cancel Automati
49. bration Value 1 mm Zoom Magnification OK If there has been a mistake in the calibration process all values in the Graph of Calibration Values will not lay on a straight line On the following image there has been an incorrect calibration of the zoom magnification 2 5 This particular value must be recalibrated Table of Magnifications Calibration Imaging Device 1 Stereomicroscope x Table of Calibration Values mm l Calibrate New Magnification Zoom Magnification Changer Magnification Recalibrate Selected Magnification Objective Position 0 7 0 8 1 1 25 16 2 1 17 1308 14 9644 12 0085 9 5681 7 4295 5 9 peateed agnifcaton Remove All Magnifications Change Objective Positions 4 m gt Graph of Calibration Values zT E 3 3 3 5 H E a gt Zoom Magnification OK 26 Calibration of the Program for Measurements 4 Setting an Active Calibration If you are using QuickPHOTO DSLR 3 1 program with multiple microscope imaging device sets and you have created in the Calibration Manager more than one calibration it is important to set the corresponding calibration as active when you change the active imaging device This particular calibration will be used for newly acquired images Setting an active calibration can be done three different ways In the Calibration Manager c
50. bration is necessary for all available magnifications of the microscope which you plan to use for your measuring What is meant by magnification of the microscope in this program is a combination of the objective s magnification and the zoom s or the magnification changer s magnification If you are using an upright or inverted microscope without the magnification changer the number of magnifications for calibration is given by the number of used objectives value of the zoom equals for all objectives If you are using an upright or inverted microscope with the magnification changer the number of magnifications for calibration equals the number of objectives x the number of magnifications of the magnification changer When using a stereomicroscope with a zoom the number of magnifications for calibration equals the number of objectives x the number of magnifications click stop positions of the zooming unit Important You will need a calibration scale a stage micrometer for the calibration 15 Calibration of the Program for Measurements 2 Calibration Manager Note What is meant by calibration in this program is the process of calibration as well as the set of calibration values for individual magnifications of the microscope imaging device sets marked by Calibration Name If you want to use various types of imaging devices or microscopes for your measurements it is necessary to calibrate create a calibration for
51. c White Balance After you have activated the Image Auto White Balance option automatic balancing of the white color will take place in the whole image 47 Main Program Window White Balance Defined by Point After you have activated the Image White Balance Defined by Point option click the left mouse button to such a point in the image which will define the reference white color for the whole image White balance based on the chosen reference value will take place in the whole image Sharpen After you have activated the Image Sharpen option the whole image will be sharpened Blur After you have activated the Image Blur option the whole image will be blurred Median Filter After you have activated the Zmage Median Filter option median filter will be applied to the whole image This function will remove some point defects such as the so called hot pixels or some types of image noise from the image This function is suitable especially for fluorescence applications Negative After you have activated the Image Negative option the image will be transferred to a negative If you repeat this function image will be brought to its original state Grey Scale After you have activated the Image Grey Scale option the image will be transferred to a grey scale image Black Balance Defined by Point After you have activated the Image Black Balance Defined by Point option click the
52. ce In the Imaging Devices menu choose the imaging device which you want to use for image acquisition In this menu you will find those imaging devices whose support was selected during the program installation Below is given the list of all imaging devices being currently supported by QuickPHOTO DSLR 3 1 program Canon EOS Digital SLR Camera option for Canon EOS series DSLR cameras Type of the camera in use will be detected automatically 2 2 Opening of the Imaging Device s Control Panel To open control panel of the selected imaging device click the icon activate the Open Imaging Device Control Panel option from the File menu or use a F4 key shortcut 2 3 Acquiring an Image In the open control panel of imaging device set the required parameters of exposition and acquire an image using the Capture button Acquired image will be immediately transferred to the computer and displayed in the Display Window For more detailed information about imaging devices control panels see Control Panels of Imaging Devices on page 95 3 Work with Images Description of work with images is divided into the following chapters 1 Image Opening 2 Image Editing 3 Indication and Annotating of Interesting Areas 4 Image Saving 5 Image Printing 43 Main Program Window 3 1 Image Opening It is possible to open images using the File Open option by clicking the icon or by a Ctrl O key shortcut
53. ce computers it is recommended to keep this function disabled Antialiasing of Inserted Objects Enabled E Backup Settings Load Settings A Configuration Wizard OK Cancel 87 Main Program Window 6 2 Section Measurements Tab Units On this tab you may choose units which will be used for measuring micrometers or millimeters For the chosen units you may also choose the number of decimal places for displaying of the measured values Important The use of measuring accuracy in tenths of micrometers will only be relevant when the used microscope imaging device set provides such accuracy Options x 4 General Default Folders Loi Image Names amp Saving um Number of Decimal Places 0 5 Sound Language mm Number of Decimal Places 2 Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger E Backup Settings Load Settings Configuration Wizard oK Cancel Tab Scale Bar This tab contains options for setting of the calibrated scale bar 88 Main Program Window In the Scale Bar Type you may set the type of the scale bar Ruler Line Segment Staple and Stripe In the Scale Bar Size you may select one of the two automatic sizes Small Large or you may select the Custom option and type in
54. croscope in use is equipped with a zoom or a magnification changer check the corresponding 18 Calibration of the Program for Measurements option Zooming Unit or Magnification Changer Unit If the microscope is not equipped with either of these elements check the No Zoom Magnification Changer option In this case the value of zoom magnification changer in this calibration will automatically be set for all objectives on the value 1 Continue by clicking the Next button A dialog box Table of Magnifications Calibration Calibration Name will open This step will finish adding of a new calibration and you may continue with the actual calibration of particular magnifications Add Calibration Imaging Device 1 Upright Microscope x Zoom Magnification Changer Selection Select whether your microscope is equipped with zooming or magnification changer unit Zoom Magnification Changer Selection No Zoom Magnification Changer Default selection for upright and inverted microscopes without magnification changer zoom 1 Zooming Unit Default selection for stereomicroscopes Magnification Changer Unit Selection for upright and inverted microscopes with magnification changer unit lt Back Calibration of Individual Magnifications In the next step calibration of individual magnifications will be done Proceed according to the following instructions 1 In the open dialog box Table of
55. d to activate the same tool over manually e Single Insertion Mode if you activate this option the selected tool will be automatically deactivated after one object has been inserted Setting the Line Color and Thickness To set the line color and thickness of the drawn objects use the Edit Object Line Color and Edit Object Line Thickness options or corresponding icon 3 7 in the Toolbar After the option Edit Object Line Color was activated or after clicking the left part of the l icon select the desired color from the displayed color palette After the option Edit Object Line Thickness was activated or after clicking the right part of the 7 H icon select the desired line thickness from the displayed list Icon 77 also serves as an indicator of the currently set line color and thickness Selected line color and thickness will be used for inserting of all new objects Draw Line Tool Draw Line can be activated by the Annotations Line option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left 52 Main Program Window mouse button or by clicking the left mouse button into the starting point and then the endpoint a line may be drawn Precisely horizontal or vertical line may be drawn if you hold the Shift key while drawing it Draw Arrow Tool Draw Arrow can be activated by the Annotations Arrow option or by clicking the icon
56. dialog box for setting of the timestamp will open 100 Control Panels of Imaging Devices Top eft Top Right Bottom eft Bottom Right Format using the options in this part it is possible to set the appearance of the timestamp Position this option enables to select into which corner of the acquired images should the timestamp be inserted Orientation using this option it is possible to select the orientation of the timestamp text 42 02 2015 15 33 07 Preview in this field preview of the timestamp is displayed the way it will be inserted into the acquired images except for the orientation option The text size is the same as will be displayed in the acquired images in the 100 1 1 display size 101 Control Panels of Imaging Devices 102 Modules Program QuickPHOTO DSLR 3 1 contains extensive functions in the form of built in modules Other optional modules can be added to the program 1 Timer Built in Module for Time Lapse Image Capturing QuickPHOTO DSLR 3 1 is equipped with the Timer module which is designed for automatic image capturing in the defined time interval the so called time lapse image acquisition This module can be activated by the Modules Timer option or by clicking the icon in the Toolbar Timer dialog box will be displayed along with the control panel of the active imaging device Timer dialog box contains a Capturing tab onl
57. e Last Used Magnification to a New Image if you activate this option each new image will automatically be assigned the last magnification assigned to an image 73 Main Program Window 4 2 Scale Bar Inserting a Calibrated Scale Bar For a basic idea about the dimensions you can insert into the current image a calibrated scale bar Option Measurements Insert Horizontal Scale Bar or the icon inserts into the right bottom corner a horizontal scale bar Option Measurements Insert Vertical Scale Bar or the l icon inserts into the right upper corner of the image a vertical scale bar By clicking again on the corresponding icon you can remove the scale bar which was inserted into the image The value displayed above the scale bar shows its full length Scale bar is inserted in a layer above the image and if you change the display size of the image scale bar size and value will continuously recalculate Scale Bar Settings If you want to change the properties of the inserted scale bar click on the scale bar with the right mouse button A context menu with several choices will appear Scale Bar Options Profiles Profile 1 Profile 2 Profile 3 Profile 4 Option Scale Bar Options opens an Options dialog box on the Scale Bar tab Edit gt Options Measurements Scale Bar This tab contains number of choices for type size and the appearance settings of the scale
58. e is only one JPEG type image on the list of images to be saved Calculate button calculates the resulting image size at the set JPEG quality Preview button displays a window with the image preview as it will look at the chosen JPEG quality Option Save As Option File Save As saves images selected in the Thumbnail Browser using the Save As window which enables to choose the location names and types of files being saved Option Save All As Option File Save All As saves all images displayed in the Thumbnail Browser using the Save As window which enables you to choose the location names and types of files being saved Window Save As Window Save As replaces the standard Windows dialo g box for saving files into a chosen location This window contains an expanded functionality and it enables saving of more images at the same time This window consists of several parts Thumbnails of Images for Saving e Location e File Name and Type e Additional File Name Tags e Flattening Layers Information 63 Main Program Window Thumbnails of Images for Saving Location Number of images to Save 4 Selected sample jpg Reem 2448 x 1920 PEG Quality 95 1 MI estan e sampiez EL lhe sampled eS samples 4140 x 3096 JPEG Quality 95 i sample5 Documents a f sample3 jpg 2546 x 1919 JPEG Quality 95 sample4 bmp 2448 x 1920
59. e name By clicking the Save button you will run the video file creation itself You may watch the process of creating of the video file on the Video Rendering page Using the Rendering Priority option it is possible to select how busy will the computer be with creating of the video file If you want to work with another programs during creation of the video file you may select the Low option You may pause creating of the video file by clicking the Pause button and you may run it again by clicking the Resume button 110 Modules GB Time Lapse Video Video Rendering Information about the rendering process is displayed on this page 111 Modules 112 List of Key Shortcuts Key shortcuts used in the Main Window of the QuickPHOTO DSLR 3 1 program Key Shortcut Action Ctrl O Open images from available disc units Ctrl S Save images selected in the Thumbnail Browser Shift Ctrl S Save As saves images selected in the Thumbnail Browser into the selected location Ctrl Alt S Save All saves all opened images Shift Ctrl Alt S Save All As saves all opened images into the selected location Ctrl W Close selected images Ctrl Alt W Close all images Ctrl P Print Ctrl Z Undo Ctrl Y Redo Ctrl C Copy Ctrl V Paste Del Delete selected objects Ctrl E Flattening Layers Ctrl I Image Information
60. e performed according to the selected reference color The Default button restores the original white balance values For manual white balance adjustment use the White Balance sliders located on the Settings tab Exposure Region in case of automatic exposure mode is set the area defined by the rectangular region displayed in the live view is used for exposure measurement The region s size cannot be changed however the frame can be moved by a mouse Using the Display Hide button it is possible to hide or display the frame If the frame is hidden the selected region is still being used for measuring of exposition The frame is only not visible in order not to disturb in the live view By clicking the Center button you may place the frame to the center of the camera s field of view The Zoom In button zooms in the area defined by the frame This functions facilitates the microscope focusing The zooming in of the area defined by the frame can be achieved also by double clicking into the frame To return to the standard display mode click the Zoom In button again or double click in the zoomed live view Settings Tab This tab is designed for setting of live view parameters 97 Control Panels of Imaging Devices Crop Borders E Frame Rate FPS 16 Power Supply AC Adapter P Settings Crop Borders D p 9 o Fra
61. each used set of microscope imaging device It is possible to switch between the created calibrations according to the actual set in use There is a program function named Calibration Manager for creating of and subsequent administration of calibrations which can be found in the menu Measurements Calibration Manager Ctrl Alt C Calibration Manager is used for creating and administrating of all calibrations created for individual microscope imaging device measuring sets Calibration Manager x Add Calibration Imaging Device 2 Stereomicroscope Rename Calibration Remove Calibration Set as Active Table of Magnifications Last Calibrations Modification 12 01 2015 11 09 44 Lock Calibrations eel Backup Calibrations Load Calibrations Close To create a new calibration click the Add Calibration button in the Calibration Manager dialog box To delete a chosen calibration from the list click the Remove Calibration button To change the name of a created calibration click the Rename Calibration button If there is more than one listed calibration calibration chosen for measuring can be activated by clicking the Set as Active button for more information see Setting an Active Calibration on page 27 16 Calibration of the Program for Measurements After a calibration is marked by the mouse click and you click the Table of Magnifications button a Table of Magn
62. ece EEEE eek eae needa a a 113 Troubleshooting FAQ Frequently Asked Questions csceceeeeeeeeeeeeeeees 115 1 Canon EOS DSLR Cameras x22 si nels yetiets cracls Woetehivinceanise eons roche 115 2 Tim Laps Video FAQ soseen esei aaisa ueia aa aa n ae iadaaa 115 System Requirement sisie creii oeei EEEa EE E EEE EE E ai 117 TR C gt EEE A AAE E see ceased we re Uhade tes 119 Installation Important It is necessary to have the administrator s privileges for installation If you do not have administrator s privileges contact your administrator When installing the QuickPHOTO DSLR 3 1 program proceed according to the following steps 1 Installation of an Imaging Device for more information see Installation of an Imaging Device on page 5 2 Installation of the QuickPHOTO DSLR 3 1 Program for more information see Installation of the QuickPHOTO DSLR 3 1 Program on page 6 Individual steps are described in the following chapters 1 Installation of an Imaging Device 1 1 Installation of Canon EOS Digital SLR Cameras 1 Connect the Canon EOS digital SLR camera to your running computer turn it on and switch the mode dial to the Av automatic mode or M manual mode position 2 Wait until the camera s device drivers are automatically installed 3 Continue with step nstallation of the QuickPHOTO DSLR 3 1 Program for more information see Installation of the QuickPHOTO DSLR 3 1 Pr
63. enus can be modified when unlocked 4 Hardware Imaging Devices Remote Trigger Ey Backup Settings I GH Load Settings Configuration Wizard OK Cancel In the Toolbar part there are two options available If you check the Display Toolbar Buttons with Captions option all icons in the toolbar will change into buttons containing also their names If all buttons do not fit on the monitor arrows which will offer the remaining buttons in the drop down menus will also be displayed in the Toolbar Example of the displayed buttons is on the following picture aw amp o o a ma a onecte 0 Zoom i Open Save Print ImageInformation Undo Redo Copy Paste Select Region Insert Horizontal Scale Bar Insert Vertical Scale Bar a P 2 44 a a 1 Ma zz o o m BestFitZoom Zoom In Zoom Actual Size 100 Crop Rotate Clockwise by 90 Object Line Color Thickness Text Line Arrow Rectangle Circe If you want to change the arrangement of the Toolbar uncheck the Lock Toolbar and Menus option Toolbar as well as Menu bar will switch to the mode when it is possible to hide or change the location of individual items This way it is possible to set your own user defined interface of the program When you finish arrangements in the Toolbar or menus lock them again by checking the Lock Toolbar and Menus option 86 Main Program Window Tab Miscellaneous In the Slideshow part it is possible t
64. er Changes 49 Main Program Window Image Adjustment x Brightness Contrast Gamma Red Green Blue E Red y 8 E Green y 3 E sue g 68 Histogram Adjustment Option Image Adjust Histogram and the icon open a Histogram Adjustment dialog box 50 Main Program Window This dialog box consists of several parts In the Channel part you can choose in which color channel of the image will the histogram adjustment take place Option Luminosity affects the whole image all color channels at the same time If you check the option Color Channels it is possible to adjust individual color channels independently In the Jnput Values part actual histogram adjustments take place Displayed graph represents a histogram for a channel selected in the Channel part Histogram scale can be changed using the vertical Scaling slider Under the histogram graph there are small triangles displayed on the edges These small triangles can be moved and thus set the border histogram values the so called black point left triangle and the so called white point right triangle Set values are displayed in editable fields under the histogram In the bottom part of the window there are image previews before and after changes have been made Buttons and between both previews are there for the change of display size
65. es selected in the Thumbnail Browser Display of all or only of selected images will take place successively in a time interval which can be chosen in the program options Option Display in Full Screen Mode will display an image images opened in the Display Window full screen You may return to the standard mode by pressing any key Measurements Annotations Imaging D Display Objects in the Current Image P Best Fit Zoom Zoomin Zoom Out 4 1 Zoom Actual Size 100 Start Slideshow Alt S Display in Full Screen Mode Ctrl F Menu Measurements Menu Measurements contains tools for calibrated scale bar insertion Insert Horizontal Scale Bar and Insert Vertical Scale Bar options insert hide the calibrated scale bar in the current image Using the option Units you may set the units using for measuring micrometers or millimeters Option Set Active Calibration enables quick switching between the active calibrations used for measuring if you have created more than one calibration for various microscope imaging device sets Option Calibration Manager opens a dialog box with functions for calibrations managing For more information see Calibration Manager on page 16 For more information about measurements see Measurements on page 72 34 Main Program Window Annotations Imaging Device Insert Horizontal Scale Bar Insert Vertical Scale Bar Units Set Active Calibration Calibration Manager Ctrl Alt C
66. es you to save besides the actual image data additional information metadata for example sensor sensitivity aperture number exposure time focal length information about the use of flash and other data thanks to the Exif extension Exif extension enables the QuickPHOTO DSLR 3 1 program to use this file type for saving of images with the layers kept as they are 59 Main Program Window Saving Parameters x Set the JPEG quality for all selected JPEG images JPEG Quality Quality 95 gt High X 1 i a i Small File Large File File Size Not Calculated Calculate le Note Default value of the JPEG quality is in the QuickPHOTO DSLR 3 1 program set at the value 95 You can set the default value by the Default JPEG Quality parameter in the Edit Options General Image Names amp Saving program setting Information about TIFF File Type TIFF file type Tag Image File Format suffix tif represents a commonly used option for image saving using a lossless compression There is no decline in quality of the saved file which makes this type of file very suitable for the need of high quality print Disadvantage of this file type is its large data size This file type does not allow the user to set the quality TIFF type enables to save not only the image information but also additional data Thanks to this it is possible to use the TIFF type to save images
67. f layers This parameter Miscelaneous affects the font size of the measured values as well as the scale bar size in the flattened image Larger window size 4 Measurements will cause smaller font and scale bar size and vice versa Default option is 1024 x 768 Units Scale Bar Window Size 1024x763 Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger E Backup Settings Gi Load Settings Configuration Wizard OK Cancel 6 3 Section Hardware Tab Imaging Devices Properties of the Imaging Device s Control Panels part contains the following options e Minimize Imaging Device Control Panel after Image Acquisition if this option is checked control panel of the imaging device will be minimized after acquiring of a new image e Display Imaging Device Control Panel on the Second Monitor for Dual Monitor Workstations if you check this option control panel of the imaging device will be displayed on the second monitor e Display Imaging Device Control Panel after Program Start up if this option is checked control panel of the imaging device will be automatically activated after launching of the QuickPHOTO DSLR 3 1 program 92 Main Program Window Properties of the Imaging Devices Control Panels except for TWAIN control panels Display Imaging Device Control Panel on the Second Monitor for Dual Monitor Workstations Options 4 General
68. f the Graphical User Interface 0c ceeeeeceeececneeeeeeeeeneeeeanees 29 Ts Menu Bates orea eat Sa ees Sees a tS OR SEIS eae 31 1 2 Toolbar 3060 05 sieriieeta ei lend Gidea in be esate 36 t 3 Thumbnail Browser rinier ig ie dieiis ehete riia rio i eainiie eR eani 40 T4Navi ator l a a Se ea ae See Sate dS 41 1 5 Display Window ccecceeeceeeceeece cece cece eeneeeeeeeuaeeeneeeeeeeeeeeseaeeeaeegas 42 1 6 Status Bar aoire tes ee L EE EEEE E E E E EEE EEE ESS 42 2 Acquiring of New Images cc cece ceeeeceeeeeeeeeeeeeeeeeeeeeeeeeeeeaeesaeeeeeeeeeees 42 2 1 Selection of the Imaging Device cceceeecceeceeeceeeceeeeeeeeaeeea tena tees 43 2 2 Opening of the Imaging Device s Control Panel 0 0 ceeeeeeeeeeeeeee neers 43 2 3 ACQGUITING am IMAGE slice Ae ids o a E Sah heginiele detox 43 3 Work with ma ges a0 iena a wad E ie Sonnets bddteannia veces E Ei S 43 3 1 Tamia Pes Opening wees sol eoeshe Go Reese os oan sa ek a a I Sa eo GAS 44 3 2 Wrage Editing 232 63 oe Bene tke cei Sade tci ae cn odes dep ades vanes beat dteatane 44 3 3 Indication and Annotation of Interesting Areas ccsceceeeeeeeeeeeee ees 51 3A limage Savino s sccd 2 cst ad eng ck Seige AE EEEE A ded dese AEEA ERE 58 3 5 IMAGE Printing oenen ee R E E ob 15 dA A A codes ee pecs 68 A Measurements 2s c0h a canceih loses soe cc0d Sak esas ih Seca gee aes ceed Sg secs ht eds 72 4 1 Preparation for Measu
69. for a stereomicroscope with one objective and a zooming unit look for example like this one Table of Magnifications Calibration Imaging Device 1 Stereomicroscope ac Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification Recalibrate Selected Magnification Objective Position 0 7 0 8 1 1 25 1 6 2 25 3 2 oo 1 PEZIS 149644 120085 9 5681 7 4295 5 9150 4 7123 3 6450 Remove Selected Magnification agnifcation Remove All Magnifications 4 M gt Graph of Calibration Values T 3 3 3 5 8 fi Zoom Magnification ok Table of Magnifications after a correctly done calibration might for a stereomicroscope with two objectives and a zooming unit look for example like this one 25 Calibration of the Program for Measurements Table of Magnifications Calibration Imaging Device 1 Stereomicroscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification Recalbrate Selected Magnification Objective Position 0 7 0 8 bo ET eT 2 2532 M 0 5 35 7593 28 4464 22 7975 17 778 14 2866 11 4085 93e6 ReTeveSelected Momnifcaton 1 7 18 3047 14 6645 11 7816 9 1630 7 3325 5 8361 4 5415 Remove All Magnifications Change Objective Positions 4E M Graph of Calibration Values 1 Cali
70. g this button an image will be acquired and automatically transferred to the computer If the camera is not connected Capture the button will change to Connect 95 Control Panels of Imaging Devices Full Screen One Point WB Image Tab Full Screen Ctrl F this button is used for displaying of live view in full screen mode Using this type of display you can control the program using the Capture Ctrl Enter button which is placed in the left upper corner of the screen and the Exit Full Screen Mode button placed in the right upper part of the screen Automatic White Balance this button activates the function for automatic white balance It is best to do this balancing when the camera is capturing a white area White Balance Defined by Point after clicking this button click the left mouse button to such a point in the image which will define the reference white color for the whole image White balance based on the chosen reference value will take place in the whole image This tab contains control elements for setting of main camera s parameters Settings will apply to the live view as well as to the acquired images i Image Image Size 4272 x 2848 ha Exposure Mode Auto Exposure Exposure Correction 0 Sensitivity ISO Auto Y Image Size Image Size from this menu you may select the size in which the 4272 x 2848
71. hoose the requested calibration from the list and click the Set as Active button Next to the name of the active calibration will appear a sign Active From the menu bar using the Measurements Set Active Calibration option Calibration will be set as active if you choose it from the displayed list This listed calibration will be marked by the check mark Insert Horizontal Scale Bar f Insert Vertical Scale Bar Units Set Active Calibration v Imaging Device 1 Upright Microscope Calibration Manager Ctrl Alt C Imaging Device 2 Stereomicroscope 27 Calibration of the Program for Measurements 28 Main Program Window Main Program Window of QuickPHOTO DSLR 3 1 program is an image editor in which images are displayed right after they were acquired or opened This editor is equipped with functions for inserting of calibrated scale bar editing and saving of images In Display Window there can be displayed only one image however the program can handle multiple opened images All opened images are displayed in the Thumbnail Browser The Navigator field makes orientation in the enlarged image easier The following functions for editing of images are available in the QuickPHOTO DSLR 3 1 program crop clockwise and counter clockwise rotation rolling along the axes resize brightness adjustment contrast adjustment color balance and gamma correction adjustment histogram adjustment
72. ifications Calibration Calibration Name dialog box containing calibration values for each magnification of the microscope imaging device set opens It is possible to lock all the calibrations from unauthorized changes with password protection For locking click the Lock Calibration button and enter new password After locking it will not be possible to make any changes in calibrations until calibrations are unlocked again by clicking the Unlock Calibration button and entering the password which was used for locking It is possible to export all the created calibrations into a backup file xml type by clicking the Backup Calibrations button If calibrations need to be restored it is possible to load the saved calibrations from the backup file by clicking the Load Calibrations button and choosing the particular file containing calibration backup In the bottom left part of the Calibration Manager dialog box there is a date and time of the last change made 3 Creating a New Calibration Before creating a new calibration put the stage micrometer under the microscope set the lowest magnification and focus the microscope The process of creating a new calibration consists of two main steps e adding a new calibration e calibration of the individual magnifications Adding a New Calibration To add a new calibration use the following procedure 1 Open the Calibration Manager dialog box by activating the Measurements gt C
73. image file Width and Height show the image size in pixels e Actual Width and Actual Height show the real size of the image in length units if the image has an assigned calibration e Quality displays the image quality in DPI scale e You may enter a text comment for the image to the Description field If the description field is filled in for the image an indicator Image contains description will be displayed next to the image thumbnail in the Thumbnail Browser If the image is saved with the layers kept description will be saved as a part of the image file 75 Main Program Window Metadata Tab Some imaging devices save into JPEG type images additional information about the image acquisition the so called metadata for example sensor s sensitivity aperture number exposure time focal length information about the use of flash and so on This tab will display metadata of the image if they are available Calibration Tab This tab will only be displayed if the image has an assigned calibration On this tab information about the assigned calibration and the microscope magnification which was used for acquiring of the image are displayed Change Image Calibration button opens a Change Image Calibration Image Name dialog box in which you can change calibration and magnification assigned to the image Calibration may also be removed from the image by clicking the Remove Calibration button
74. indow It is also possible to change the area size using the green grasp points When dragging the corner grasp points with a mouse while holding the Shift key ratio of the area dimensions will be preserved It is possible to move the chosen area by dragging the mouse while holding the left mouse button by the inside of the chosen area In case of need it is possible to cancel the Crop function by pressing the Esc key or by clicking the right mouse button rectangle defining the area will disappear Cropping itself is done by a mouse double click on the inside of the chosen area or by clicking on the 4 icon Only the chosen part of the image will remain The rest of it will be removed Copying Image or its Part to the Clipboard To copy the whole image displayed in the Display Window to the clipboard activate the B Edit Copy option click the icon or you may use the Ctrl C key shortcut If you want to copy only a part of the image displayed in the Display Window to the y clipboard activate the Edit Select Region option or click the icon first select the desired area for copying to the clipboard same way as was described in the previous paragraph Image Cropping To copy the chosen area to the clipboard activate the Edit G Copy option click the icon or use the Ctrl C key shortcut Pasting Image from the Clipboard Content of the clipboard if it contains image information can
75. ine Color and Object Line Thickness Option Restore Original Image will restore the original form of the image in which it was opened or acquired all inserted objects will be deleted Option Delete Selected Objects will delete all selected objects from the current image Option Delete All Objects will delete all inserted objects from the current image Option Merge Objects with an Image Flatten Layers will merge layers containing inserted objects with the image After merging it will no longer be possible to edit the inserted objects they will become a fixed part of the image Option Configuration Wizard will open a guide for basic program setup For detailed description of work with Configuration Wizard see Configuration Wizard on page 7 Option Options will open a dialog box for setting of program parameters For detailed description of the setting options see Options on page 78 32 Main Program Window Edit Image View Measurements Annotations Imaging Devic Undo CtreZ Copy Ctrl C Paste Cart TP Select Region T Object Line Color Object Line Thickness Restore Original Image X Delete Selected Objects Del Delete All Objects Merge Objects with an Image Flatten Layers Ctri E Configuration Wizard Options F11 Menu Image In the menu Zmage it is possible to display Image Information There are also available the following options for image editing crop rotatio
76. ing the Finish button If prompted restart your computer Initial Configuration of the Program First Run of the Program When you run the QuickPHOTO DSLR 3 1 program for the first time a Configuration Wizard will open which will help you to configure the program correctly Settings can be later changed manually by the Options from the menu Edit Options or by repeated running of the Configuration Wizard from the Edit gt Configuration Wizard menu Important Full completion of the configuration using the Configuration Wizard is in case of the first run of the QuickPHOTO DSLR 3 1 program necessary When you finish the Configuration Wizard the QuickPHOTO DSLR 3 1 will run automatically 1 Configuration Wizard This wizard consists of several pages through which you can browse using the Next and Back buttons When configuring proceed according to the wizard s guidelines Imaging Device Selection Second page of the wizard offers the choice of imaging device which will be used for image acquiring You can make your selection among only those devices whose support was selected during program installation Select the desired imaging device and click the Next button Default Folders Next page of the wizard enables to set the folder which will be offered as default when saving images This way you will not have to browse the whole directory structure of the computer to select the desired folder where you wan
77. iod of 30 days from installation After 30 days the trial period will expire and the program cannot be used anymore until the license information will be entered You will obtain the license information from the QuickPHOTO DSLR 3 1 program supplier after purchasing its full license For entering the license information click the Enter License Information button at the welcome screen Following dialog box will appear Enter License Information x In this dialog box enter the license information for activation of QuickPHOTO DSLR 3 1 program full version You will obtain the license information from your QuickPHOTO DSLR 3 1 program supplier after purchasing its full license License Information User Name Activation Code 13 Entering License Information Enter the license information you obtained into the corresponding fields an click the OK button You will be informed about successful product registration After that you can start using the full version of the QuickPHOTO DSLR 3 1 program 14 Calibration of the Program for Measurements 1 Opening Information about Calibrations QuickPHOTO DSLR 3 1 program is equipped with following measuring features insertion of horizontal or vertical calibrated scale bar Before it is possible to start measuring in the QuickPHOTO DSLR 3 1 program it is necessary to calibrate the program for a particular microscope imaging device set Cali
78. jection of images will be suspended To continue the presentation press the Space key Parameters of the presentation can be adjusted in the Options of the program Edit gt Options General Miscellaneous For the presentation you can set the delay between images and the way of its termination 5 3 Display in Full Screen Mode Option View Display in Full Screen Mode displays the content of the Display Window full screen You may return back to the original way of display by pressing any key by a mouse double click or a right mouse button click 77 Main Program Window 6 Options Edit Options F11 option opens an Options dialog box with three main sections General Measurements and Hardware and it contains all options for settings of the program For application of the made settings click the OK button After clicking the Cancel button made settings will not be applied The whole setting of the QuickPHOTO DSLR 3 1 program can be backed up into a file of the xml format by the Backup Settings button in the bottom part of the Options window If you need to restore the program settings you can do so by loading of the backup file after clicking the Load Settings button Configuration Wizard button opens the Configuration Wizard for more information see Configuration Wizard on page 7 Individual sections of the Options window will be described in the following chapters 6 1 Section Genera
79. k drives flash memory and so on Ctrl O fq Save saves an image images selected in the Thumbnail Browser Ctrl S amp Print initiates printing of selected images Ctrl P Image Information displays information about the current image Ctrl 1 gt Undo undoes the last performed image editing Ctrl Z cu Redo does again the last undone image editing Ctrl Y Copy inserts a selected area or a whole image displayed in the Display Window into a clipboard Ctrl C Paste inserts the clipboard content as a new image Ctrl V 36 Main Program Window clicking and dragging the mouse while holding the left mouse button Objective Objective drop down menu for the choice of the objective which was used to acquire the image It is used to assign the correct calibration for measurements Zoom Zoom drop down menu for the choice of zooming unit magnification changer magnification which was used for acquiring of the image It is used for assigning of the correct calibration for measurements If you are not using either the zooming unit or the magnification changer value 1 will automatically be filled into this field after you choose an objective Insert Horizontal Scale Bar inserts a calibrated horizontal scale bar into the right bottom corner of the image Insert Vertical Scale Bar inserts a calibrated vertical scale bar into the right upper corner of the image Open Imaging Device Control
80. l This section contains the following tabs Tab Default Folders This tab enables to set the folder which will be offered as default when saving images This way you will not have to browse the whole directory structure of the computer to select the desired folder where you want to save the files Default folder can be changed anytime Standard dialog box for folder selection will open after clicking the Change button Change of the folder can also be done by typing in of the desired path into the corresponding field If the selected folder does not exist it will be created 78 Main Program Window Options x 7 Fis Default Folders Image Names amp Saving Default Folder for Saving Acquired Images Sound C Program Files QuickPHOTO DSLR 3 1 Images Change Language Do Not Show This Window Default Folder for Saving Time Lapse Images Annotations C Program Files QuickPHOTO DSLR 3 1 Sequences Change Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger E Backup Settings fa Load Settings Configuration Wizard Tab Image Names amp Saving Names of New Images part contains preferences for naming of new images Default name of all new images acquired by the imaging device can be determined by typing in of a text string into the Acquired Images field Serial number will automatically be attached to
81. lso a green grasp point connected by a dash line with its center Using this point you can rotate the ellipse any way you wish To change the size shape of the selected object proceed as follows move the mouse cursor to one of the green grasp points Cursor will change to By clicking on the grasp point and by dragging the mouse while holding the left mouse button you can change the size shape of the object Deleting of Inserted Objects and Text Annotations In order to edit inserted objects select them first for more information see Selection of the Inserted Objects for Editing on page 56 57 Main Program Window To delete a selected object from the image press the Del key activate the Edit Delete Selected Objects option or click the icon To delete all not only selected objects inserted objects from the image activate the Edit gt Delete All Objects option or click the 7 icon 3 4 Image Saving Information about Work with Layers QuickPHOTO DSLR 3 1 program uses for work with inserted objects an independent layer above the image Thanks to this function it is possible to edit hide or delete all inserted objects There are two possible ways to save the images with layers kept as they are or with flattened merged layers The saving option named Flattening Layers is available in the Saving Parameters and Save As windows and contains the following options 1 Keep Inserted Objects in
82. mage capturing Turn off the screen saver of the Microsoft Windows operating system Switch the automatic updates function to the manual installation mode Use the computer s UPS Before you start capturing images into the folder make sure that you are authorized to write into the folder and there is enough free space on the target drive Because with most illuminators the illumination intensity decreases after turning on until temperature stabilization let the illuminator warm up and thermally equilibrate before you start the time lapse capturing 2 Time Lapse Video Built in Module Time Lapse Video module which is a standard component of the QuickPHOTO DSLR 3 1 program is designed for creating of video files from the acquired time lapse images For example if you acquire a series of images of a slowly changing sample with the Timer module you can then easily create using the Time Lapse Video module a video file in which the movement will be displayed in an accelerated form How to Create a Time Lapse Video To create a video file proceed according to the following steps 1 If you intend to create a video file from images currently opened in the QuickPHOTO DSLR 3 1 program select them in the Thumbnail Browser Run the Zime Lapse Video module from the Modules Time Lapse Video menu or click the icon in the Toolbar The Time Lapse Video wizard will open 106 Modules On the Selection of Image
83. me Rate FPS 16 Power Supply AC Adapter White Balance S R e 9 gt 0 94 cje 1 01 Bie o gt 0 97 Orientation ee Mirror 6 Flip Rotate by 180 at Cross Grey Scale Crop Borders this slider enables to crop the unwanted borders from the image symmetrically from all sides Use this function in case the darker corners are visible in the live view This phenomenon called vignetting may appear when using smaller magnifications on some types of microscopes The performed setting is interactively displayed in the live view and it will apply also to the acquired images This function does not have any effect on measurement accuracy Frame Rate displays how many images per second Frames Per Second FPS is the camera currently displaying in the live view Power Supply this indicator displays the camera s battery status or the use of the AC power supply 98 Control Panels of Imaging Devices White Balance these sliders enable to manually adjust white balance by adjusting the level of each of the three color channels R red G green and B blue S Restore Default White Balance Settings clicking this button sets all values of the White Balance sliders to the default values Orientation Orientation in this part you may set the orientation of the live view al A Theperformed setting is interactively displayed in the live view and 6
84. me4apse images On this page select source of images for processing and click the Next button Source Selection Process Images Selected in the Thumbnail Browser Select Saved Files for Processing Up to 2000 Files Select the Whole Folder for Processing Next gt Cancel Next page of the wizard Video Properties enables to set parameters of a new video file There are two options affecting the speed of the captured action available e Interval between Frames this option enables to set the time interval between images This option is useful for example if you want to have control over the speed of the projected action compared to reality e Frame Rate this option enables to create a video file with the desired length or fluency To create a fluent video file the minimum value of 25 images per second FPS is recommended According to the set parameters the Video Duration will be calculated In the Video Resolution part you may select the size of the resulting video file in pixels The larger size you select the larger will be the resulting video file 108 Modules WB Time Lapse Video x Video Properties On this page select parameters of a new video file and dick the Start button Video File Settings Interval between Frames 40 ms Frame Rate Video Resolution 768x576 px Video Duration 0 00 07 Timestamp o V Date
85. n flip mirror resize automatic white balance white balance defined by point sharpen blur median filter negative grey scale black balance defined by point and functions for brightness adjustment contrast adjustment gamma correction color balance adjustment and histogram adjustment For detailed description of individual functions see Image Editing on page 44 Image View Measurements Annotations Imagi Image Information Ctrl I Crop Rotate Clockwise by 90 Rotate Counter Clockwise by 90 Flip Mirror SBEnAAIAS Resize a Auto White Balance r al r a White Balance Defined by Point Ctrl T Sharpen Blur Median Fitter Negative Grey Scale Black Balance Defined by Point XD Adjust Brightness Contrast Gamma Adjust Colors al Adjust Histogram 33 Main Program Window Menu View Option Display Objects in the Current Image will display hide all objects inserted into the current image Option Best Fit Zoom will display the current image in such a size so it would fit into the Display Window Options Zoom In and Zoom Out will enlarge reduce display of the current image by one step Option Zoom Actual Size 100 will display the current image zoomed in by 100 so that one image pixel corresponds with one pixel of the monitor Option Start Slideshow will activate full screen view of all the opened images in case only one image is selected or imag
86. n line Step 2 Enter the actual length of the drawn calibration line and dick the Finish button ee Ce Actual Length of the Line Segment 600 um mm 5 Set another magnification for calibration on the microscope Change the objective in use or set a different value of magnification of the zooming unit magnification changer 6 Complete calibration of the microscope imaging device set for all available microscope magnifications is done by repeating this procedure steps 1 to 5 The result of this procedure should be a situation when all the points in the Graph of Calibration Values in the Table of Magnifications Calibration Calibration Name dialog box will lay on a straight line respectively on straight lines If some of the graph points significantly stand out from the line it means that there has been a mistake in the calibration process In this case choose the deviating magnification by clicking the relevant value in Table of Calibration Values or by clicking the deviating point in the Graph of Calibration Values and click the Recalibrate Selected Magnification button Then in a similar manner as in steps 2 to 4 recalibrate the chosen magnification to the correct value Table of Magnifications after a correctly done calibration might for an upright microscope without the magnification changer look for example like this one 23 Table of Magnifications after a co
87. ng and verification of the remote trigger s functionality Remote trigger is an optional accessory which can be connected via serial or USB port If you want to use the remote trigger check the Use Remote Trigger option and select the COM port to which it is connected in case of the USB trigger we are talking about the USB Serial Port COM In the part Remote Trigger Type select the type of the used remote trigger Trigger with two buttons for example the two button foot switch is designed for image acquiring and for capturing of video recordings using the optional module for video recording Functionality of the remote trigger can be verified by pressing its button s If the small square s change color to green after you press the button s and they turn back to black after you release the button s the remote trigger is set correctly and it is ready to be used To continue click the Next button Initial Configuration of the Program amp Configuration Wizard x QuickPHOTO Remote Trigger In this step a remote trigger optional accessory can be set DSLR 3 1 Vv Use Remote Trigger com Select a COM port to which is the remote trigger connected 2 Verify the remote trigger s functionality by pressing its button s The black square s should turn green Color Theme Selection On this page of the wizard you may select color theme of the program To continue click the Next button
88. ns 1 Canon EOS DSLR Cameras Which position of mode dial should be set to control the camera from a computer Switch the mode dial to Av position for automatic mode or to M position for manual mode How to change Auto Power Off time setting Canon EOS DLSR cameras come from manufacturing preset for switching to standby mode automatically after 30 seconds of inactivity When the camera is in the standby mode it cannot be connected by QuickPHOTO DSLR 3 1 program To activate the camera again press the shutter button The following procedure describes how to change the time after which the camera goes to standby mode for Canon EOS 1100D DSLR Camera The procedure for other models is similar You can find more information in the user guide of the camera Turn on the DSLR camera and disconnect the USB cable from it Press MENU button and use arrows to select the first tab with yellow wrench symbol Select Auto power off option and press SET button Use arrow keys to select the desired value and confirm by SET button The recommended option is OFF 2 Time Lapse Video FAQ It is not possible to play the video file created using the Time Lapse Video module Install a codec from the QuickPHOTO DSLR 3 1 program installation disk or the extracted folder from the Codec folder to the computer on which you want to play the created video file You may also download the codec for free from the website www promicra com downloads php
89. o be used 93 Main Program Window Options 4 General Default Folders Image Names amp Saving Sound Language Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements oo Units Scale Bar Sizes Colors Other Settings 4 Hardware Remote Trigger V Use Remote Trigger Test i Port cOM1i Pc Settings IIM 1 Select a COM port to which is the remote trigger connected 2 Verify the remote trigger s functionality by pressing its button s The black square s should turn green Single Button Trigger i i Two Button Trigger Load Settings A configuration Wizard 94 Control Panels of Imaging Devices 1 Canon EOS Digital SLR Cameras Canon EOS 1100D Digital SLR Camera ame TE Timestamp na 2 Capture Full Screen Auto WB One Point WB mae settings E x BO Sn a 4272 x 2848 The control panel works with Canon EOS series digital SLR cameras Control panel of the Canon EOS digital SLR cameras is designed for control of camera functions displaying of the live view and image acquisition It consists of a toolbar several tabs with control elements and a live view Toolbar The Toolbar which is located above the live view contains the following buttons Capture Ctrl Enter after clickin
90. o set Delay Between Images in seconds Default delay is set at three seconds If the Terminate Slideshow after the Last Image Display option is not checked the last image of the presentation will remain displayed on the monitor Maximum image size which can be opened in the QuickPHOTO DSLR 3 1 program or which can be acquired can be set in the Maximal Image Size part In the Antialiasing of Inserted Objects measurements drawings annotations the feature for smoothing of inserted objects can be enabled disabled Enabling of this feature slows down the display performance especially in case when there are multiple objects inserted into an image therefore this feature is not recommended for slower computers Options x 4 General Default Folders Sdetow Image Names amp Saving Delay Between Images 3 sis Sound Language Terminate Slideshow after the Last Image Display Do Not Show This Window Annotations Maximal Image Size Appearance and Layout Choose the maximum size of images that can be opened in the program Miscellaneous 4 Measurements Units a Maximal Image Size in Megapixels Sizes Colors ximal Image Size in Megapixels Unlimited T Other Settings 4 Hardware Antialiasing of Inserted Objects measurements drawings annotations Imaging Devices ES Trigger This function enables smoothing of inserted objects edges however it influences the display performance For low performan
91. ogram on page 6 Installation 2 Installation of the QuickPHOTO DSLR 3 1 Program Note Administrator s privileges are required for QuickPHOTO DSLR 3 1 program installation If you do not have the administrator s privileges contact your administrator After signing in with the administrator s privileges proceed as follows 1 In case you are going to install the QuickPHOTO DSLR 3 1 program using a downloaded installation package run it and select the destination folder where the installation files should be extracted Wait until all files will be extracted A home screen should appear automatically Then click the Install QuickPHOTO DSLR 3 1 button at the home screen Setup Wizard of the QuickPHOTO DSLR 3 1 program will open Continue according to the on screen guidelines On page Select Components Imaging Devices select the support of the desired imaging devices Continue according to the on screen guidelines Onpage Select Additional Tasks part select the device drivers which should be installed for the used devices if applicable On page Select Additional Tasks File Types Associations it is possible to set association of the QuickPHOTO DSLR 3 1 program for opening of files of the BMP JPEG PNG or TIFF types If you check one or more of the mentioned options the QuickPHOTO DSLR 3 1 program will always be used for opening of the corresponding file types Finish the installation by click
92. omatic saving of JPEG type images and it will also be used as the default setting for manual image saving Tab Sound This tab offers the option of setting the sound played during acquiring of images If the Play a Sound when Image Has Been Acquired wav file option is checked a sound file of the wav type will be played when acquiring an image To choose the sound file click the Change button and select the desired file After clicking the button the selected file will be played Options x 4 General Default Folders Sound Image Names amp Saving V Play a Sound when Image Has Been Acquired wav file Sound meri C Program Files QuickPHOTO DSLR 3 1 trigger wav P change Do Not Show This Window Annotations Appearance and Layout Miscellaneous 4 Measurements Units Scale Bar Sizes Colors Other Settings 4 Hardware Imaging Devices Remote Trigger E Backup Settings Load Settings Configuration Wizard Cancel 82 Main Program Window Tab Language This tab offers the option to choose a language of the QuickPHOTO DSLR 3 1 program To change the language select the desired language from the list and click the Change Language button This operation may take a while Options x 4 General Default Folders bannuoge Selection Image Names amp Saving Language Notice Some program parts may not be translated to all languages
93. on will affect all images to be saved The first option Keep Inserted Objects in a Separate Layer is valid only for JPEG and TIFF type images With the BMP and PNG types layers will always be merged automatically Part Information In the nformation part explanatory notes are displayed for indicators and warnings about important facts about saving of images Information The red labeled images have the same name and type It is necessary to rename some images before saving Ay Images with yellow exclamation mark already exist in the current folder and will be overwritten Some images are going to be saved as BMP bmp or PNG png type These types do not support saving of layers Inserted objects will be merged with image Saving of Images When Exiting the Program When exiting the QuickPHOTO DSLR 3 1 program you will be asked whether you wish to save the not yet saved images Option Save called from the displayed message window works the same way as the option File Save All 67 Main Program Window 3 5 Image Printing Program QuickPHOTO DSLR 3 1 enables to print 1 4 images next to each other on one page Select images for printing max 4 in the Thumbnail Browser and activate the File Print option or click the icon Function Print can also be activated for a particular image by clicking the Print B button in the controls of the corresponding thumbnail Note If you select more than
94. ose images selected in the Thumbnail Browser respectively all opened images Option Open Imaging Device Control Panel will open a separate window for acquiring images using the imaging device For more information about work with control panels of the imaging devices see Control Panels of Imaging Devices on page 95 Option Print will open a dialog box for image printing For more information about print see Image Printing on page 68 Option Exit will end the program 31 Main Program Window Fle Edit Image View Measurements Annotatio fj Open Ctr 0 Open Recent gt save Ctr s A Save As Shift Ctrl S save all Ctrl Alt S E Save AllAs Shift Ctri Alt S Close Selected Images CtrleW Close All Images Ctrl Alt Ww Open Imaging Device Control Panel FA amp Print Ctrl P Exit Alt X Menu Edit The following options are available in the Edit menu Undo will take back the last performed action It is possible to take back as many consecutively performed actions as you wish Option Redo will again perform the action taken back using the Undo function Option Copy inserts an image or its chosen part into a clipboard Option Paste will insert the content of the clipboard as a new image Option Select Region is used for selection of a part of an image to be copied into a clipboard Change of color and thickness of the lines of the objects being inserted can be done using the options Object L
95. r Settings Mode of Assigning Magnifications to Images part offers two options Manual Magnification Assignment to Each Image if this option is active default option it is necessary to manually set magnification from the Objective or eventually Zoom menu to each image intended for measurements Automatically Assign the Last used Magnification to a New Image if you activate this option to each new image will be automatically assigned the last magnification assigned to some image In the Settings for the Function Merge Objects with an Image Flatten Layers part it is possible to set the size of the virtual window which will be used for flattening of layers Setting of the Window Size value has affect on the font size of the measured values and scale bar size in the flattened image Larger window size will cause smaller font and scale bar size and vice versa Default option is 1024 x 768 pixels 91 Main Program Window Options x 4 General a Default Folders tode of Assigning Magrilications to Images Image Names amp Saving Manual Magnification Assignment to Each Image Sound Language Automatically Assign the Last Used Magnification to a New Image Do Not Show This Window Annotations Settings for the Function Merge Objects with an Image Flatten Layers Appearance and Layout Here it is possible to set the size of the virtual window which will be used for flattening o
96. ring cceeceeeceeeceeeceeeceeeeneeeaeeee eee eeaeeenees 73 AZ Cale Bath souzcnisnc nae ean e aar a asses a e a been ao iaeoa hatae lenges 74 5 Other Functions Aeysiesstivgs Ge Sel este Set ede pal pnts os ees a oh deste Suk es sade 75 5 1 Image Information cc cece cece ceeece cece cece eee eeae eeu eeeeeeeeeeneeeeseeeeges 75 QuickPHOTO DSLR 3 1 D2 Sideshow siro Breana a sdedetunsiteheedsd sebiwid tecbeag a a aad EEA NEEE En 77 5 3 Display in Full Screen Mode ecceecc cece ence eeceeeceeeeeeeeneeeneeeaeeneeees 77 OXOPtlons sc 2 eee e a talbott Pen sak aA Bea eked a Ee 78 6 1 Section General miseire oeio exten hs ec vedas SEEE e tee eaneebad ceca E 78 6 2 Section Measurements cecceecceeceeeceeeceeeeeeeee cece eeseeeaeeeeeeeaneeanes 88 6 3 Section Hard wate cece vacecges i EE E da vecas ve E EEE E E a 92 Control Panels of Imaging Devices cecccecceeceneeeeeceeeeeeeeeeeeeeeeeeeneeeaeene nega 95 1 Canon EOS Digital SLR Cameras oecon oeii iii i e 95 Mod leSe yi mie enaner eanan e e a a aaa e e a a 103 1 Timer Built in Module for Time Lapse Image Capturing 0 068 103 Ll Tab Capturing erene a a AA bo Sask eh EE a alae 103 1 2 Recommendations for the Time Lapse Image Capturing 66 106 2 Time Lapse Video Built in Module 0 cece ceecceeeceeeeeeeeeeueeeueeeaaes 106 List f Key Shortcuts osien ds Hes tev c
97. rl Alt sS Save All As Shift Ctrl Alt S Option Save Option File Save or the icon in the Toolbar will save only those images selected in the Thumbnail Browser If the images being saved have already been saved before they will be saved again into their original locations If the JPEG or TIFF types of images are being re saved into their original locations a Saving Parameters dialog box will be displayed Other types of images BMP and PNG will be re saved automatically If you are saving newly acquired images a Save As dialog box will be displayed which will enable 61 Main Program Window you to choose the location names and types of the files being saved If all selected images have already been saved this option will be inactive Option Save All Option File Save All saves all images displayed in the Thumbnail Browser If the images being saved have already been saved before they will be saved again into their original locations If the JPEG or TIFF types of images are being re saved into their original locations a Saving Parameters dialog box will be displayed Other types of images BMP a PNG will be re saved automatically If you are saving newly acquired images a Save As dialog box will be displayed which will enable you to choose the location names and types of files being saved If all images have already been saved this option will be inactive Saving Parameters Window Saving Parameters window will be
98. rrectly done calibration might for an upright microscope Calibration of the Program for Measurements Table of Magnifications Calibration Imaging Device 1 Upright Microscope x Table of Calibration Values mm Calibrate New Magnification Zoom Magnification Changer Magnification Objective Position 1 4 ss 0 0712 20 0 0356 En 0 0178 Recalbrate Selected Magnification Remove Selected Magnification Remove AllMagnificatons Change Objective Positions Graph of Calibration Values 1 Calibration Value 1 mm Objective Magnification ok with the magnification changer look for example like this one Table of Magnifications Calibration Imaging Device 1 Upright Mic with Mag Changer x Table of Calibration Values mm Calibrate New Magnification H Zoom Magnification Changer Magnification Recaibrate Selected Magnification Objective Position 1 2 4 r Por Remove Selected Magnification 10 0 7150 0 3609 Remove All Magnifcations 20 0 3588 0 1807 Change Objective Positions 40 0 1785 0 0896 Graph of Calibration Values 3 3 3 2 3a g Objective Magnification OK 24 Calibration of the Program for Measurements Table of Magnification after a correctly done calibration might
99. rval option set the Beginning and End of the time interval intended for time lapse image capturing Beginning and end of the interval is defined by the date and time The beginning and end date can be defined by typing in from the keyboard or by selecting it from a calendar which can be displayed by clicking the arrow on the right from the displayed date Time values can be entered from the keyboard or using the arrows on the right hand side next to the corresponding time fields Then in the Interval between Images part set the desired time interval in hours minutes and seconds Note Minimal interval between images depends on the size of the images being captured and the set exposure time of the active imaging device If you set an interval shorter than is the time necessary for image capturing and its transfer to the computer you will be warned about this fact and the time interval for image capturing will be set at a minimum applicable value Location of the Captured Images Location of the captured images can be selected in the Store Images part There are two options available e in the Main Program Window images will be displayed after capturing in the Main Program Window this option is suitable for capturing of a smaller number of images e in Folder this option is recommended for capturing of larger number of images Images will be after capturing automatically saved into the selected folder and they will not be
100. see Selection of the Inserted Objects for Editing on page 56 The color and line thickness of selected objects may be changed For these purposes use the following options Edit Object Line Color Edit Object Line Thickness or the corresponding icon 3 in the Toolbar New line color of the selected objects can be chosen from the displayed palette after the option Edit Object Line Color was activated or after clicking the left part of the Enk icon 56 Main Program Window New line thickness of the selected objects can be chosen from the displayed list after the option Edit gt Object Line Thickness was activated or after clicking on the right part of the 7B icon Relocation Change of Size and Shape of the Drawn Objects Relocation of Text Annotations In order to edit inserted objects select them first for more information see Selection of the Inserted Objects for Editing on page 56 If you want to move the selected object in the image move the mouse cursor to its contour line Cursor will change to P By clicking on the object s outline and by dragging the mouse while holding the left mouse button the object can be moved to the desired area In the middle of selected objects of the ellipse rectangle and circle types there is a cross Using this cross you may also move these objects in the above mentioned way Use a similar process to move text annotations In the ellipse there is a
101. t contains two options of the page orientation Portrait and Landscape To print one and four images next to each other the default orientation will be landscape to print two images next to each other the default orientation will be portrait Color Mode part contains two options of the print mode Color and Grey Scale Set the number of copies in the Copies part In the Units part choose units for setting of margins and the print size You can choose between cm or inches In the Margins part set the desired page margins for printing Minimal Margins button will set the minimal margin size which the currently selected printer allows Page Layout Tab Original Size from Image DPI Fit to Page Keep Aspect Ratio Stretch to Page No Aspect Ratio Image Alignment Image Information Image Name Position P o S Date and Time of Image eat tesa Origin o a o Description gt ar le a lo fer fo Post oO Oo o Image Borders Image Spacing On off Space 0 5 cm 70 Main Program Window Image Size part contains the following options Original Size from Image DPI this option sets the original print size according to the image DPI QuickPHOTO DSLR 3 1 program assigns to the acquired images the 300 DPI quality Fit to Page Keep Aspect Ratio this option uses the whole page area for printing and it keeps the original aspect ratio of the image Stretch to
102. t to save the files To continue click the Next button Initial Configuration of the Program lt Configuration Wizard x QuickPHOTO Default Folders DSLR 3 1 In this step default folders can be defined Default Folder for Saving Acquired Images C Program Files QuickPHOTO DSLR 3 1 Images Default Folder for Saving Time Lapse Images C Program Files QuickPHOTO DSLR 3 1 Sequences Scale Bar and Units On this page of the wizard you may select the type and size of the calibrated scale bar In the part Scale Bar Type you may set the type of the scale bar Ruler Line Segment Staple and Stripe In the part Scale Bar Size you may select one of the two automatic sizes Small Large or you may select the Custom option and type in your own desired length of the scale bar into the field Custom Size In the part Preview you will see what the scale bar will look like when using the current settings In the part Units select units in which the measured values will be displayed micrometers or millimeters To continue click the Next button Initial Configuration of the Program Configuration Wizard QuickPHOTO Scale Bar and Units In this step a scale bar type and units used for measurements can be defined DSLR 3 1 Scale Bar Type Line Segment 2 Scale Bar Size for Q Small Auto Size Remote Trigger This page is used for setti
103. the Zmage Borders part select whether the printed images should have a black frame When printing more images on one page it is possible to set the Image Spacing in the chosen length units 71 Main Program Window Buttons in the Bottom Part Default Settings button returns all settings in the Print window to the original values Print button will begin print on the selected printer it will close the Print window and it will also store the set parameters for further opening of the Print window Cancel button will close the Print window without storing the set parameters Apply button will store the set parameters for further opening of the Print window actual print will not take place Note Print parameters are always stored only within the current QuickPHOTO DSLR 3 1 program run 4 Measurements QuickPHOTO DSLR 3 1 program is equipped with functions for inserting a calibrated scale bar 4 into images Note To use the functions for measurements it is necessary to calibrate the QuickPHOTO DSLR 3 1 program first for more information see Opening Information about Calibrations on page 15 To achieve correct measuring results it is necessary to have set the corresponding active calibration for the microscope imaging device set in use for more information see Setting an Active Calibration on page 27 72 Main Program Window 4 1 Preparation for Measuring Selecting M
104. tical edges can be drawn if you hold 53 Main Program Window the Shift key To finish drawing of the polygon click the right mouse button the last vertex will be connected with the first one and that way the polygon will be closed Draw Freehand Tool Draw Freehand is activated by the Annotations Freehand option or by clicking the icon Then by clicking into the image and by dragging the mouse while holding the left mouse button draw the desired curve Insert Text Annotation Tool Insert Text Annotation is activated by the Annotations Text option or by clicking the 4 icon Cursor will change into a cross with the letter A Then click the left mouse button into the image to the spot where the text annotation should be placed A Text Annotation dialog box will open Text Annotation x Background Color Orientation Transparent Custom Color Abc Abc oqy Background Area Rectangle Behind Text Only Bl z UlA 35 gt aval 222 Sample text aqy u m Type the text content into the bottom field Setting of the font properties can be done using the Bold E Italic Z or Underline buttons Font color can be chosen from the color palette which will show after clicking the Text Color amp button Font size and type can be selected from the A a drop down menus To align a multiline text p g 54 Main Program Window
105. tion for Calibration Current Objective Magnification 0 5 Zoom Magnification Changer Magnification 1 6 Cancel Calibration of the new magnification stereomicroscope with a zooming unit 3 Next page of the guide 2 Image Source Selection offers a choice whether to acquire the calibration image from the active imaging device or whether to use the already existing image opened in Display Window In the Image Source part choose Acquire a New Image from the Imaging Device Continue by clicking the Next button 21 Calibration of the Program for Measurements 4 Calibrate New Magnification Objective 10 Zoom 1 x 2 Image Source Selection Select the source of images for calibration In case of acquisition of a new image from a microscope place a stage micrometer under the microscope Image Source Acquire a New Image from the Imaging Device Imaging Device Type Ceme Cancel Control panel of the active imaging device with a live view of the stage micrometer opens Place the micrometer horizontally to the center of the imaging device s field of view and focus the microscope Click the Capture button Next page of the guide 3 Calibration will open with the image of the stage micrometer If the quality of the calibration image is not satisfactory it is possible to open the imaging device control panel again to acquire a new calibration image using the icon In
106. use the following buttons Align Multiline Text Left Center Multiline Text and Align Multiline Text Right EEZ Orientation of the text can be chosen in the Orientation part by clicking the corresponding button with the Abc text on it Orientation Abc Abc oqy aqy Background of the text annotation can be set in the Background Color and Background Area parts If you in the Background Area part check the Transparent option text annotation will be inserted into the image with a see through background If you check the Custom Color option it will be possible after clicking the button to choose the annotation s background color from the displayed color palette After checking the Custom Color option the Background Area part with two choices will be made available Option Rectangle will fill in the whole rectangle area defined by the text annotation with the set background color Option Behind Text Only will fill in only the area behind text with the set background color To insert a text annotation click the OK button Note Text writing and all above mentioned editing are continuously displayed directly in the image Parameters of the text annotation can be selected interactively If the window Text Annotation covers the inserted text annotation in the image we recommend to move this window to a different area by dragging it by its upper strip Inserted text annotation can be easily moved around in the
107. y in other programs for work with images the image will be displayed with all inserted objects If you open such an image in the QuickPHOTO DSLR 3 1 program it will no longer be possible to edit the inserted objects in any way The image will not contain information about the used calibration and the image s description will not be available To save images with flattened layers you can use all supported image types JPEG TIFF PNG as well as BMP Types of Files Supported by the QuickPHOTO DSLR 3 1 Program QuickPHOTO DSLR 3 1 program works with images of the JPEG TIFF PNG and BMP types The following paragraphs contain basic information about these file types Information about JPEG File Type JPEG Quality The JPEG Joint Photographic Experts Group suffix jpg file type is the most commonly used type of file for saving photomicrographic images This is a file type utilizing the so called lossy compression Result of this is a relatively small data file size at the expense of loss of image quality This loss of quality however means no problems for most photomicrographic applications Quality of the saved image can be affected by a parameter named JPEG quality This parameter has influence on the level of JPEG compression that way it affects the quantity of information details which will be preserved in the image Higher JPEG quality means lower level of compression that way a larger saved file and vice versa JPEG type enabl
108. y in a default state If you have activated a computer controlled illuminator in Edit Options gt Hardware Illuminators the Timer dialog box will also contain a second tab Illumination After setting of the desired parameters described in the following chapters run the time lapse a Start image capturing by clicking the button Time lapse image capturing can be terminated at any time by clicking the Sad lutte In the bottom part of the Timer dialog box there are the following information displayed Time until Next Capturing Number of Captured Images and Number of Remaining Images Note If the Timer module for some reason will not be able to capture images in the defined time interval the Time until Next Capturing label will be red In such case a text file containing information about the delay or possibly omission of the concerned images will be created 1 1 Tab Capturing Capturing tab contains control elements for the setting of parameters of the time lapse image capturing You may select from two modes 103 Modules e Number of Images Time Interval Image Capturing in the Number of Images Mode After you select the Number of Images mode set the number of images you want to capture in the Number field In the Interval between Images part set the desired time interval in hours minutes and seconds Image Capturing in the Time Interval Mode After you select the Time Inte
109. y last one of your selection while holding the Shift key Discontinuous selection of images in the Thumbnail Browser can be done by selection of desired thumbnails with the left mouse button while holding the Ctrl key Selection of all images at the same time can be done by the Ctrl A key shortcut Canceling of the selection of more thumbnails can be done by clicking the left mouse button on a random thumbnail Order of thumbnails may be changed by dragging them with a mouse drag and drop To gain a larger work area you may hide slip in to the left the Thumbnail Browser by clicking the E button in its right upper corner This button then will change to button 40 Main Program Window This way the Thumbnail Browser will free up and if you move the mouse cursor outside of the Thumbnail Browser it will be slipped in to the left In this case you may work with Thumbnail Browser by moving the mouse onto the vertically oriented Thumbnail Browser tab on the left side of the Main Program Window Thumbnail Browser will be pulled out Its anchoring may be done by clicking the button FA Thumbnail Browser Image Count 4 Selected 1 sample 1 jpg 2448 x 1920 sample2 jpg 4140 x 3096 sample3 jpg 2546 x 1919 o ajali x sample4 jpg Sy 2447x 1919 1 4 Navigator In the left bottom part of the Main Program Window there is a Navigator displayed with the current image In
110. your own desired length of the scale bar into the Custom Size field Color of the scale bar and its value can be chosen in the Scale Bar Options part by the Color of Scale Bar and its Value option Options Background Area and Background Color can change the area and color of the scale bar s background In the Preview part you can immediately see what will the scale bar look like when using the current settings User defined settings of the scale bar can be saved into one of the four profiles To save the user defined settings to a profile proceed as follows 1 Make all the desired scale bar settings 2 Click the Save button in the Profiles part Save Profile dialog box will open 3 Select one of the four profiles you want to save the settings to from the Save Settings to Profile drop down menu 4 Enter the new profile name into the New Profile Name field 5 Save the settings by clicking the OK button If you check the nsert Scale Bar into Image Automatically after Magnification Assignment option in the Automatic Scale Bar Insertion part a scale bar will be inserted into images automatically after magnification which was used for image acquisition has been assigned You can select whether to insert the Horizontal Scale Bar or the Vertical Scale Bar Note Font size of the scale bar s values is set on the Measurements Sizes Colors tab 89 Main Program Window
111. zes Image opened in the Thumbnail Browser can be displayed in the Display Window by clicking the left mouse button on the thumbnail or by dragging of the thumbnail drag and drop into the Display Window If you move the mouse over the thumbnail of one of the opened images several buttons will appear in its right upper part These buttons activate functions only for this particular image Save Image la button will save the image Print B button will print the image Image Information Li button displays information about the image including meta data if those are available description and possible assigned calibration Mark as Important t button will frame the thumbnail with a red frame for its highlighting Image can be closed using the Close button If some changes have been done to the image you will be asked whether you want to save these changes before you close this image New images that have not yet been saved and images that have been edited will have an asterisk displayed in front of their name for example P00001jps If the description of an image is filled in there will be an indicator Image contains description displayed by the thumbnail Selection of one image in the Thumbnail Browser can be done by one click of the left mouse button on the corresponding thumbnail Continuous selection of several images in the Thumbnail Browser is possible by clicking the mouse on the first thumbnail and then on the ver

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