Eppendorf ThermoMixer F1.5 and FP Operations Manual
Contents
1. 2 222eeeeeeeeeoenn 15 2 ege 14 0 Operating controls ssssse 8 14 P A a 0 09 6 POERUDIAIO iiec ont eerte rre 6 16 Power connection socket 8 Power switch 2 2222eeeeesecccsnnnnnnnnnnnn 8 R Uu 6 S Safety precautions sess 10 Select location eren 13 Set temperature Display 15 Short Mix 14 Short M X aerae aei 18 Speaker Symbol 7 6707 76 15 Storage 77 7 25 T Technical data Ambient conditions Application parameters Power supply a Weights dimensions Temperature I 14 Temperature control 4419 Temperature key eene 14 Tempering Application parameters 27 Switching off the temperature control 18 Symbol 7 70 Thermoblock Eppendorf ThermoMixer F1 5 FP Operating manual dato e e e E 6 17 15 TUBOS 7 EE 16 nie 8 V Verification of temperature control 24 Volume IMP ee 20 Warning sign 7707 13 5 5 EE E 6 31 jenuew unedo Operating manual 32 Eppendorf ThermoMixer F1 5 FP Operating manual LS ce ceY RT gt e ect gt q ET ce ct gt ce ce ce C 7 gt gt at gt 6 HA KG2. 1 ER RU 8 2 2 4 ThermoMixer F1 5 iii 77 7 7 1 90 8 2 2 2 ThermnoMIxet FP ioe ome ea ube oes 8 2 3 Eeer AE EE id 9 3 Safety 8989 7 7 1 1 9 3 4 Intended USC 11 11 9 3 2 User profile esn Ai 3 bdo tb on on P 9 3 3 Information on product ab 10 3 4 Warnings for intended use 10 3 5 Warning signs on the device 24 4 4 13 4 Installation een 13 4 4 Selecting the location 13 4 2 Installing the instrument lille 13 Operation RR 14 5 1 Overview of operating 00 666 14 5 2 Inserting tubes and plates 0 0 eee 16 5 3 Installing the ThermoTop 0 0c ccs 17 UNA DID 3 3 3 23 3 333 do dee when a tans 18 5 4 1 Mixing without temperature Control 18 5 4 2 Mixing and tempering 0 cc cece eee eee Rh 18 um Ec dq 27 7 2838 111 090 18 5 5 rei A ee e det 19 5 5 1 Temperature control without mixing process 19 5 6 Men lt bean dd Rae ER Rs 20 5 6 1 Navigating in the men 20 5 6 2 Menu structure rasera e eR RR RE ERU ee a ns 20 5 7 Loading saved parameters 44ee 21 jenuew 40 Eppendorf ThermoMixer F1 5 FP Operating manual EN 6 Troubleshooting mE REESE 3333 ERE 1 59 22 641 General iors e io e epi edo hae et Ca
3. If you cannot remedy an error with the recommended measures please contact your local Eppendorf partner The contact address can be found online at www eppendorf com worldwide 6 1 General errors Symptom Cause Remedy message Display remains No mains connection gt Check the mains connection dark and the power supply gt Switch on device Set temperature is Set temperature is less than 4 C gt Set up the device in a cooler not reached above ambient temperature environment ThermoTop LED The interface between the gt Remove any dirt from the front does not light device and the ThermoTop is of the ThermoTop dirty gt Remove any dirt from the top of the device especially from the viewing window beside the heating cooling plate ThermoTop does The lid is attached to the gt If using the ThermoTop do not not fit on the device thermoblock use the lid The device does Various causes are possible gt Contact your local Eppendorf not mix or control partner the temperature 6 2 Error messages Symptom Cause Remedy message Error message Various causes are possible 1 Switch off device and wait preceded by a 10 seconds number code 2 Switch on device If the error message appears again contact your local Eppendorf partner Eppendorf ThermoMixer F1 5 FP Operating manual 7 Maintenance 7 1 Cleaning Clean the housing of the ThermoMixer F1 5 ThermoMixer FP regularly
4. Eppendorf ThermoMixer F1 5 FP Operating manual eppendorf Copyright 2013 Eppendorf AG Hamburg Germany No part of this publication may be reproduced without the prior permission of the copyright owner eppendorf Eppendorf Thermomixer and Eppendorf ThermoTop are registered trademarks of Eppendorf AG Hamburg Germany condens protect is a trademark of Eppendorf AG Hamburg Germany Trademarks are not marked in all cases with TMor in this manual 5384 900 013 01 012013 Eppendorf ThermoMixer F1 5 FP Operating manual Table of contents 1 Heer instructions ei En CH nenne an ELE 8 5 1 1 Usingthis manual enm an ea 5 1 2 Danger symbols and danger lees 5 1 2 1 Danger symbols ee sr ehe eph Rh a 5 1 2 2 Danger levels 66 5 133 Symbols used 55 6 1 4 Abbreviations use 6 ED leet ree 1 1 bis 6 2 Product description 3 2 6 nsr RU Rn EROR nen 7 2 1 Mainillustratlon 2202 n Enp NEE EEN E NEPA A 7 2 2 Delivery package 17 7 7
5. Set the mixing frequency Keep the arrow key pressed quick setting Display menu enter key Open the menu Confirm your selection Eppendorf ThermoMixer F1 5 FP Operating manual 1 3 4 p Ole 3 alza 200 C rpm u 7 6 Fig 4 Display ThermoMixer F1 5 ThermoMixer FP 1 Device status 2 ThermoTop lt x Device is performing mixing temperature ThermoTop has been attached control To prevent the formation of condensate the device heats up the ThermoTop before the temperature of the thermoblock is controlled Speaker Key lock d Speaker switched on u Key lock activated Parameters cannot be IK Speaker switched off changed u No key lock Mixing frequency Actual temperature When the actual temperature flashes on the display the device is not in temperature control mode operation Set temperature When the set temperature has been reached only one value is displayed 15 Eppendorf ThermoMixer F1 5 FP Operating manual EN 5 2 Inserting tubes and plates NOTICE Damage to plates due to too high temperatures Polystyrene microplates melt at temperatures above 70 C Polypropylene deepwell plates deform at temperatures above 80 C Deformed plates can become detached from the thermoblock gt Only heat microplates up to 70 C gt If you are heating deepwell plates above 80 C do not exceed the mixing frequency of 1000 rpm Operating manu
6. Temperature Verification System Single channel for Mastercycler Mastercycler ep Mastercycler pro and Mastercycler nexus Thermomixer compact Thermomixer 0055 000 298 950008059 comfort Thermostat plus 29 jenuew 40 Operating manual 30 Eppendorf ThermoMixer F1 5 FP Operating manual A Actual temperature eessess 15 Application parameters 27 220 23 condens protect tns 17 D Decontamination eese 24 Deepwell plate esses 6 16 70 5 5 8 15 Disposal rnnt 25 G GloSSaly diri rte 6 H Hazatds 10 Heating CT 27 Installation DID 2 33 3 311 adai 13 Selecting the location 13 Intended use RER te EE 10 K Key lock Menu arrow key Navigation Overview 3 3 3 nennen Microplate i siii en ii cene trece nte MIXING oct cee 2 2certstvezesevetsderdzee Application parameters Setting the mixing frequency 18 Symbol tes E temperature control run without temperature control 18 Mixing frequency
7. SA de ECK eur Kon T REES TES c c TEL ce Ct ce E GER Kr G K Ar ce ag uk on CC o ce ce te ECC formi ka TEL ce CE Hi ck ce CE onf it ve ze ce ce OK ot T ormi tserklz ag as a o P Li L fe x ce ity De arun c A M pM clar g 2 ct c Si rr enn Normen Be erer nt atio Ch we named bolow eegenen Kor c be 9 emu te pnmo e zm 33 DEER en e ac cs a ge ERE to the product 4 ce gt u an ce ck ce at c ea ck T ce c KO d d amp Versand PRINT 2 Board ot Gar u npn q ve ck c nip C ce ct et Ke dr ug ct C ACH CA KA ot T re CE TE sag V gz S Shs ruts Se Hi d et Evoendort Rr A NM Kou D K dias fe c6 C en t Cc Shey at ee 4 c CS TELA E c HA TES c gt T TES ze C C TE c E ng ct Operating Manual Evaluate your operating manual www eppendorf com manualfeedback eppendorf Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 53801 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Hauppauge N Y 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe Tel 49 1803 666 789 Preis je nach Tarif im Ausland 9 ct min aus dem dt Festnetz Mobilfunkh chstpreis 42 ct min support
8. eppendorf com North America Tel 1 800 645 3050 E mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com
9. 1 500 rpm ThermoMixer FP You can select mixing frequencies between 300 rpm and 2 000 rpm e 2DMix Control technology provides for controlled and efficient mixing to ensure rapid and complete mixing of even the smallest volumes Short Mix Short uncomplicated mixing of sample material The mixing process runs at the selected speed as long as the short key is pressed Lid and ThermoTop The Lid ensures uniform temperature control and protects samples from unwanted exposure to light The ThermoTop prevents the formation of condensation on the inner wall or the lid of the tube thanks to the condens protect technology 3 Safety 3 4 Intended use The ThermoMixer F1 5 ThermoMixer FP is intended for use in a molecular biology laboratory The ThermoMixer F1 5 ThermoMixer FP is designed for the temperature control and mixing of liquids in closed tubes and closed plates for the preparation and processing of samples The ThermoMixer F1 5 ThermoMixer FP is exclusively intended for use indoors All country specific safety requirements for operating electrical equipment in the laboratory must be observed Only use Eppendorf accessories or accessories recommended by Eppendorf 3 2 User profile The device and accessories may only be operated by trained and skilled personnel Before using the device read the operating manual carefully and familiarize yourself with the device s mode of operation jenuew unedo Operating manual
10. 10 Eppendorf ThermoMixer F1 5 FP Operating manual 3 3 Information on product liability In the following cases the designated protection of the device may be compromised Liability for any resulting property damage or personal injury is then transferred to the operator The device is not used in accordance with the operating manual The device is used outside of its intended use The device is used with accessories or consumables which are not recommended by Eppendorf The device is maintained or repaired by people not authorized by Eppendorf The user makes unauthorized changes to the device 3 4 Warnings for intended use Read the operating instructions and observe the following general safety information before using the ThermoMixer F1 5 ThermoMixer FP gt wo wow v DANGER Risk of explosion Do not operate the device in areas where work is completed with explosive substances Do not use this device to process any explosive or highly reactive substances Do not use this device for processing any substances which could generate an explosive atmosphere DANGER Electric shock as a result of penetration of liquid Switch off the device and disconnect the power plug before starting cleaning or disinfection work Do not allow any liquids to penetrate the inside of the housing Use closed tubes and closed plates Do not spray clean spray disinfect the housing Only plug the devi
11. DANGER Electric shock as a result of penetration of liquid gt Switch off the device and disconnect the power plug before starting cleaning or disinfection work Do not allow any liquids to penetrate the inside of the housing Use closed tubes and closed plates Do not spray clean spray disinfect the housing Only plug the device back in if it is completely dry both inside and outside vv v v NOTICE Damage from the use of aggressive chemicals gt Do not use any aggressive chemicals on the device or its accessories such as Strong and weak bases strong acids acetone formaldehyde halogenated hydrocarbons or phenol gt Ifthe device has been contaminated by aggressive chemicals immediately clean it by means of a mild cleaning agent NOTICE Corrosion from aggressive cleaning agents and disinfectants Do not use corrosive cleaning agents aggressive solvents or abrasive polishes gt Do not use lab cleaners with sodium hypochlorite Auxiliary equipment Lint free cloth Mild soap based lab cleaner Dist water Cleaning the ThermoMixer F1 5 ThermoMixer FP 1 Switch off the ThermoMixer F1 5 ThermoMixer FP and disconnect it from the power supply 2 Clean all of the outer parts of the ThermoMixer F1 5 ThermoMixer FP with a mild soap solution and a lint free cloth 3 Wipe off the soap solution with dist water 4 Dry all cleaned parts 23 jenuew 40 EN Operating manual 24 Eppendo
12. Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According to these regulations any devices supplied after August 13 2005 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste To document this they have been marked with the following identification Because disposal regulations may differ from one country to another within the EU please contact your supplier if necessary 25 jenuew 40 EN Operating manual 26 Eppendorf ThermoMixer F1 5 FP Operating manual 9 Technical data 9 1 Power supply Power connection Power consumption Overvoltage category Degree of pollution Protection class 100 V 130 V 10 50 Hz 60 Hz 220 V 240 V 10 50 Hz 60 Hz Maximum 200 W ll 2 9 2 Weight dimensions Dimensions Weight Width 20 6 cm 8 1 in Depth 30 4 cm 12 0 in Height ThermoMixer F1 5 17 0 cm 6 7 in ThermoMixer FP 16 4 cm 6 5 in ThermoMixer F1 5 6 3 kg 13 9 Ib ThermoMixer FP 6 1 kg 13 4 Ib 9 3 Ambient conditions Ambience Ambient temperature Relative humidity Atmospheric pressure Only for use indoors 5 C 40 C 10 90 non condensing 79 5 kPa 106 kPa Eppendorf ThermoMixer F1 5 FP Operating manua
13. al NOTICE Material change of consumables due to extreme temperatures Extreme temperatures e g during refrigeration or autoclaving affect consumables material The mechanical strength dimensions and shape of the consumable will change Use consumables that are suitable for the selected temperature range or selected procedure The height sensor of the ThermoMixer FP automatically differentiates between deepwell plates and microplates gt When inserting microplates make sure that the height sensor is not covered gt Take care that the height sensor does not get contaminated Inserting the plate gt Insert the plate with the back edge first Then press it down at the front Inserting tubes Insert the tubes completely into the bores of the thermoblock Only ThermoMixer FP To ensure uniform temperature control place the Lid on the thermoblock 1 Height sensor 16 Eppendorf ThermoMixer F1 5 FP Operating manual EN 5 3 Installing the ThermoTop The condens protect technology available with ThermoTop prevents the formation of condensation on the inner wall or the lid of the tube oO Prerequisites E Tubes or plates have been inserted s Place the ThermoTop on the device 8 vertically from above The centering pins 3 behind the heating plate fit into the recesses D of the ThermoTop t5 The ThermoTop is correctly positioned if the seal is fully flush with the upper pa
14. arger volume than microplates Suitable for the preparation mixing centrifuging transporting and storing of solid and liquid samples Lid Lid for the thermoblock Ensures uniform temperature control and protects samples from unwanted exposure to light Microplate Plate with 24 48 96 or 384 wells for the preparation EEEEE mixing centrifuging transporting and storing of solid 5 and liquid samples PCR plate Plate with 96 or 384 wells for PCR applications ThermoTop Heated cover for the thermoblock Prevents the formation of condensation on the inner wall or the lid of the tube thanks to the condens protect technology Well Cavity Microplate PCR or deepwell plate tube Eppendorf ThermoMixer F1 5 FP Operating manual 2 Product description Main illustration ThermoMixer F1 5 N GE D ThermoMixer FP jenuew unedo Operating manual Eppendorf ThermoMixer F1 5 FP Operating manual 1 Operating controls 2 Display 3 Thermoblock 4 Centering pins 5 Power switch 6 Power connection socket 7 USB interface for Eppendorf Service only 2 2 Delivery package 2 2 1 ThermoMixer F1 5 Quantity Order no Order No Description international North America 5384 000 012 5384000020 ThermoMixer F1 5 with thermoblock for 1 5 mL tubes 1 5384 900 013 Operating Manual ThermoMixer F1 5 ThermoMixer FP 1 5384 900 021 Short Instructions ThermoMixe
15. ce back in if it is completely dry both inside and outside WARNING Electric shock due to damage to device or mains cable Only switch on the device if the device and mains cable are undamaged Only use devices that have been properly installed or repaired In case of danger disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or by using the isolating device intended for this purpose e g emergency stop switch in the laboratory WARNING Lethal voltages inside the device Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident Do not remove the housing of the device Do not allow any liquids to penetrate the inside of the housing Do not allow the device to be opened by anyone except service personnel who have been specifically authorized by Eppendorf Eppendorf ThermoMixer F1 5 FP Operating manual EN WARNING Risk from incorrect supply voltage Only connect the device to voltage sources which correspond to the electrical requirements on the name plate gt Only use sockets with a protective earth PE conductor and suitable power cable 9 S WARNING Risk of burns from hot surfaces The thermoblock can be very hot after heating and cause burns gt Avoid direct contact with a heated thermoblock 3 3 c CG WARNING Damages to health due to infectious liquids and pathogenic germs gt Wh
16. cription international North America ThermoMixer F1 5 with thermoblock for 1 5 mL tubes 5384 000 012 220 V 240 V 5384000020 100V 130 V ThermoMixer FP with thermoblock 5385 000 016 220 V 240V 5385000024 100V 130 V 5308 000 003 5308000003 ThermoTop with condens protect technology 5363 000 233 5363000233 Lid for ThermoMixer FP 10 2 Tubes and plates Order no Order No Description international North America Eppendorf Safe Lock Tube 1 5 mL 1 000 pieces 0030 120 086 clear Eppendorf Deepwell Plate 384 200 pL 40 plates wells clear 0030 521 102 951031003 PCR Clean white border color Eppendorf Deepwell Plate 96 500 uL 40 plates wells clear 0030 501 101 951031801 PCR Clean white border color Eppendorf Deepwell Plate 96 1000 pL 20 plates wells clear 0030 501 209 951032603 PCR Clean white border color 28 Eppendorf ThermoMixer F1 5 FP Operating manual Order no Order No Description international North America Eppendorf Deepwell Plate 96 2000 pL 20 plates wells clear 0030 501 306 951033405 PCR Clean white border color All plates are available with different border colors red yellow green and blue and purity qualities in large packs as well as with barcoding on request You can find further information in our catalog or on our website www eppendorf com 10 3 Temperature sensor Order no Order No Description international North America
17. e menu enter key 5 6 2 Menu structure Menu items and options Description Key lock Key lock on Parameters cannot be changed Key lock off Parameters can be changed Volume The signal tone for error messages is always output at medium volume level regardless of the speaker settings Set the volume of the speaker Volume 1 Volume 2 Volume 3 Switching the speaker off Volume off Back Go to next higher menu level 20 Eppendorf ThermoMixer F1 5 FP Operating manual 5 7 Loading saved parameters The 37 C to 95 C keys can be used to quickly select a temperature for a temperature control procedure for an unlimited period of time Use the temp off key to switch off temperature control Temperature Mixing frequency Key 37 C 37 C off Key 42 C 42 C off Key 56 C 56 C off Key 95 C 95 C off Key temp off off off gt To call a saved temperature press a direct selection key 37 C to 95 C The LED above the key lights blue The display shows saved parameters gt To start temperature control press the start stop key gt To perform temperature control and mixing at the same time also set the mixing frequency using the speed arrow keys To exit the displayed parameters set different values for the temperature or mixing frequency 21 jenuew 40 EN Operating manual 22 Eppendorf ThermoMixer F1 5 FP Operating manual 6 Troubleshooting
18. ee Faces 22 6 2 EFrror Messages 44 22 E 7 Maintenance 3 as 0 eisen 23 5 7 1 Cleaning 0 00 c cc ccc cece cece eect e 23 E 7 2 Disinfection Decontamination ernennen 24 7 3 Decontamination before shipment 0 00 0 eee 24 E 7 4 Verification of temperature Control 24 2 8 Transport storage and disposal 2 25 x 8A Transpo sec se CTI TETTE 25 9 2 126 2 111 1 11 25 6 tat 25 9 0 7 1 26 9 1 Power supply x 7 0 26 9 2 Wegbticdnensiong 0 ccc ee Rh hn 26 9 3 Ambient conditions 11 859 26 9 4 Application parameters 27 9 4 1 T mpenng see NN EN EN NERT ANE NEE dE d ema meee 27 94 2 MIXING EECHER 27 9 5 Interface 11 1 09 27 10 Ordering information 2 0000 cee eee 28 10 1 Device and accessories 7 7 7 7 7 7 nn 28 10 2 Tubes and plates 2r eer ae 28 10 3 Temperature sensor rs RR n b RR ek em baee e he Regn 29 NT E E EEE E E Rs 30 Eppendorf ThermoMixer F1 5 FP Operating manual 1 User instructions 1 1 Using this manual g
19. en handling infectious liquids and pathogenic germs observe the national regulations the biological security level of your laboratory the material safety data sheets and the manufacturer s application notes gt Wear personal protective equipment gt For comprehensive regulations about handling germs or biological material of the risk group Il or higher please refer to the Laboratory Biosafety Manual source World Health Organisation Laboratory Biosafety Manual in its respectively current valid version WARNING Risk of fire gt Do not use this device to process any highly flammable liquids WARNING Damage to health due to contaminated device and accessories gt Decontaminate the device and the accessories before storage and shipping WARNING Risk of injury due to incorrect consumables Poorly fitting tubes or plates can become detached from the thermoblock Glass tubes can smash Only use the thermoblocks with the consumables designed for them gt Never use tubes made of glass or other fragile material WARNING Contamination due to opening seals of consumables In the following cases the seals of tubes can spring open Sample material can escape high vapor pressure of the content improperly sealed cover damaged sealing lip improperly fastened foil Always check that consumables have been sealed tightly before use 11 Operating manual 12 Eppendorf ThermoMixer F1 5 FP Operating man
20. immediately starts to perform the temperature control 2 Setthe mixing frequency with the speed arrow keys qi g 1000 C rpm 3 In order to start the mixing process press the start stop key The amp symbol flashes on the display The display shows the actual temperature set temperature and the mixing frequency 4 In order to end the mixing process press the start stop key The display shows the last used parameters Temperature control is continued 5 4 3 Short Mix Use the Short Mix function for mixing for a short while without temperature control 1 Set the mixing frequency by means of the speed arrow keys 2 Keep the short key pressed The mixing process continues as long as the short key will be pressed 3 In order to end Short Mix release the short key Eppendorf ThermoMixer F1 5 FP Operating manual EN 5 5 Temperature control NOTICE Damage to electronic components due to condensation Condensate can form in the device after it has been moved from a cool environment to o a warmer environment 3 After installing the device wait at least for 3 h Only then connect the device to the s mains 3 SE The ThermoMixer F1 5 ThermoMixer FP can be used for temperature control in a range of 4 C 3 above the ambient temperature to 100 C S E to i When the actual temperature flashes on the display the device is not in temperature contr
21. inst direct sunlight The mains power switch and cutting unit of the mains power line must be easily accessible during operation e g residual current circuit breaker 4 2 Installing the instrument WARNING Risk from incorrect supply voltage Only connect the device to voltage sources which correspond to the electrical requirements on the name plate Only use sockets with a protective earth PE conductor and suitable power cable 1 Place the ThermoMixer F1 5 ThermoMixer FP onto a suitable work surface Position the device in such a way that the ventilation slots on the underside of the device are not obstructed 2 Connectthe power cable to the power connection socket of the device and the power supply 13 EN Operating manual 14 Overview of operating controls Eppendorf ThermoMixer F1 5 FP Operating manual 5 1 SS 8 7 6 Fig 3 Operating controls Temperature keys with control LEDs Select a temperature or switch off temperature control start stop key Start or stop mixing temperature control Arrow keys temp Set temperature Keep the arrow key pressed quick setting As soon as the target temperature is modified the device begins to perform temperature control Menu arrow key Navigate the menu Set the key lock or the volume A short key Short Mix runs as long as the short key is being pressed see p 18 Arrow keys speed
22. l EN 9 4 Application parameters 9 4 1 Tempering Temperature control Minimum 4 C above ambient temperature 2 range Maximum 100 C 2 Temperature setting 1 C 100 C adjustable in steps of 1 C 3 Temperature accuracy Set temperature Set temperature a 20 C 45 C lt 20 Cor gt 45 C 3 3 ThermoMixer F1 5 0 5 C 0 5 C S ThermoMixer FP 1 0 C 4 0 C Temperature homogeneity In the range of 20 C 45 C max 0 5 C for all positions of the thermoblock Heating rate ThermoMixer F1 5 11 C min ThermoMixer FP 18 C min The change of temperature in filled tubes is slower 9 4 2 Mixing Mixing frequency can be set in increments of 50 rpm ThermoMixer F1 5 300 rpm 1 500 rpm ThermoMixer FP 300 rpm 2 000 rpm 9 5 Interface USB interface For Eppendorf service only 27 Operating manual Eppendorf ThermoMixer F1 5 FP Operating manual 10 Ordering information CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf 10 1 Device and accessories Order no Order No Des
23. o the display due to mechanical pressure gt Do not apply mechanical pressure to the display NOTICE Damage to electronic components due to condensation Condensate can form in the device after it has been moved from a cool environment to a warmer environment After installing the device wait at least for 3 h Only then connect the device to the mains NOTICE Damage from the use of aggressive chemicals Do not use any aggressive chemicals on the device or its accessories such as strong and weak bases strong acids acetone formaldehyde halogenated hydrocarbons or phenol gt Ifthe device has been contaminated by aggressive chemicals immediately clean it by means of a mild cleaning agent Eppendorf ThermoMixer F1 5 FP Operating manual EN 3 5 Warning signs on the device Risk of burns from hot surfaces Upper device side o E s Gg 3 Observe the operating manual Rear of the device 3 3 c Caution Risk of injury from moving parts On the gt Observe the operating manual thermoblock 4 Installation 4 1 Selecting the location Select the location for the device according to the following criteria Suitable power connection as per the name plate Minimum distance to other devices and walls 10 cm 3 9 in Bench with a horizontal and even work surface which is designed to support the weight of the device Surrounding area must be well ventilated Location must be protected aga
24. ol mode operation As soon as the set temperature is changed with the temp arrow keys the device begins to perform temperature control When the set temperature has been reached the display only shows one value 5 5 1 Temperature control without mixing process 1 To switch off the mixing function use the speed arrow keys to select the 0 rpm setting v below 300 rpm or A above 1 500 rpm or 2 000 rpm di y NEST 0 C rpm 2 Using the temp arrow keys set the temperature The device immediately starts to perform temperature control When the set temperature is not changed the actual temperature flashes on the display and the device does not perform temperature control 3 To manually start the temperature control procedure press the start stop key The amp symbol flashes on the display The display shows the actual temperature set temperature 19 Eppendorf ThermoMixer F1 5 FP Operating manual EN 5 6 Menu 5 6 1 Navigating in the menu To change settings proceed as follows I E ts e To open the menu press the menu enter key E D 2 Select the menu item with the menu arrow key E E 5 3 e To confirm your selection press the menu enter key 2 4 Change the settings with the menu arrow key 5 e To confirm the changed setting press the menu enter key A tick appears in front of the setting 6 To exit the menu level select the Back menu item and press th
25. r F1 5 ThermoMixer FP 2 2 2 ThermoMixer FP Quantity Order no Order No Description international North America 5385 000 016 5385000024 ThermoMixer FP with thermoblock 5384 900 013 Operating Manual ThermoMixer F1 5 ThermoMixer FP 5384 900 021 Short Instructions ThermoMixer F1 5 ThermoMixer FP i Check the delivery for completeness gt Check all parts for damage in transit To safely transport and store the device keep the transport box and packing material 1 5363 000 233 5363000233 Lid for ThermoMixer FP Eppendorf ThermoMixer F1 5 FP Operating manual 2 3 Features With the ThermoMixer F1 5 ThermoMixer FP two basic sample preparation applications can be easily performed in a single step simultaneous mixing and temperature control of the sample material The ThermoMixer F1 5 allows the temperature control and mixing of liquids in 1 5 mL tubes e g Eppendorf Safe Lock Tubes The ThermoMixer FP allows the temperature control and mixing of liquids in all common plate formats e g Eppendorf Microplates or Eppendorf Deepwell Plates Temperature control Temperatures of 4 C above the ambient temperature to 100 C are accurately and consistently maintained The temperatures 37 C 42 C 56 C 95 C can be selected directly Mixing Anti spill technology prevents lid wetting and cross contamination ThermoMixer F1 5 You can select mixing frequencies between 300 rpm and
26. rf ThermoMixer F1 5 FP Operating manual 7 2 Disinfection Decontamination DANGER Electric shock as a result of penetration of liquid gt Switch off the device and disconnect the power plug before starting cleaning or disinfection work gt Do not allow any liquids to penetrate the inside of the housing gt Use closed tubes and closed plates gt Do not spray clean spray disinfect the housing Only plug the device back in if it is completely dry both inside and outside Auxiliary equipment Lint free cloth Disinfectant Switch the ThermoMixer F1 5 ThermoMixer FP off and isolate from the power supply Allow the device to cool down Clean the device see Cleaning on p 23 Select a disinfection method which complies with the legal requirements and regulations applicable to your range of application 5 Wipe the surfaces with the lint free cloth and disinfectant Boo gw 7 3 Decontamination before shipment If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following WARNING Risk to health from contaminated device 1 Observe the information in the decontamination certificate You find it as a PDF file on our website www eppendorf com decontamination 2 Decontaminate all the parts you would like to dispatch 3 Include the fully completed decontamination certificate in the package 7 4 Verification of temperat
27. rt of the device The blue LED of the ThermoTop lights The Fflsymbol appears in the display 0 Functioning principle of the ThermoTop n order to prevent the formation of any condensate in a reliable manner the device first heats the ThermoTop until it reaches the set temperature The tempering of the thermoblock occurs with a delay The temperature sensor of the thermoblock reacts to the temperature of samples after inserting samples into a pre heated thermoblock the displayed actual temperature may fall temporarily While the device is tempering the blue LED of the ThermoTop is flashing 17 Operating manual 18 Eppendorf ThermoMixer F1 5 FP Operating manual 5 4 Mixing You can select the mixing frequency between 300 rpm and 1 500 rpm ThermoMixer F1 5 or 2 000 rpm ThermoMixer FP The mixing frequency can be adjusted in steps of 50 rpm 5 4 1 Mixing without temperature control 1 In order to switch off the temperature control press the temp off key qi g att 1000 SE rpm m Set the mixing frequency by means of the speed arrow keys In order to start the mixing process press the start stop key The amp symbol flashes on the display e 4 Inorder to end the mixing process press the start stop key The display shows the last used parameters 5 4 2 Mixing and tempering 1 Setthe temperature with the temp arrow keys The device
28. t Read this operating manual completely before using the device for the first time Please also note the operating instructions for the accessories if applicable gt This operating manual is part of the product Thus it must always be easily accessible 9 Enclose this operating manual when transferring the device to third parties gt If this manual is lost please request another one For the current version please refer to our webpage www eppendorf com worldwide international or www eppendorfna com North America 1 2 Danger symbols and danger levels The safety instructions in this manual appear with the following danger symbols and danger levels 1 2 1 Danger symbols A Biohazard Explosion A A Electric shock Hot surface Hazard point Risk of fire Crushing Material damage 1 2 2 Danger levels DANGER Will lead to severe injuries or death WARNING May lead to severe injuries or death CAUTION May lead to light to moderate injuries NOTICE May lead to material damage jenuew 40 Eppendorf ThermoMixer F1 5 FP Operating manual EN 1 3 Symbols used Symbol Meaning Ki gt Handling gt 5 1 Actions in the specified order E 2 2 D List S 8 Text Name of fields in the software o i Useful information 1 4 Abbreviations used PCR Polymerase chain reaction rpm Revolutions per minute in rpm 1 5 Glossary Deepwell plate Plate with 48 96 or 384 wells with a l
29. ual WARNING Injury from sample material being thrown out Sample material can be thrown out of open improperly sealed or unstable tubes and plates Only mix in closed tubes and closed plates gt Observe the nationally prescribed safety environment when working with hazardous toxic and pathogenic samples Pay particular attention to personal protective equipment gloves clothing goggles etc extraction and the safety class of the lab CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf CAUTION Crush hazard due to moving parts Do not replace any consumables during the mixing process gt Put on the ThermoTop or Lid prior to the mixing process Do not remove the ThermoTop or Lid during the mixing process NOTICE Caution Strong vibration When mixing at high speeds items located near the device may be moved by the vibrations of the work surface and e g fall off the work table Do not place easily movable items near the device or secure them adequately NOTICE Damage t
30. ure control To verify the temperature accuracy of the thermoblock use the Eppendorf Temperature Verification System Single Channel In combination with the temperature sensor for the ThermoMixer F1 5 ThermoMixer FP the exact temperature in the thermoblock can be measured Details on the verification process with the Eppendorf Temperature Verification System Single Channel can be found in the corresponding operating manual Eppendorf ThermoMixer F1 5 FP Operating manual 8 Transport storage and disposal 8 1 Transport CAUTION Bodily injury due to lifting and carrying heavy loads The device is heavy Lifting and carrying the device can lead to back injuries gt The device must be transported by least two people gt Use a transport aid e g dolly to transport the device longer distances gt Use the original packaging for transport Air temperature Relative humidity Atmospheric pressure General transport 25 C 60 C 10 96 75 96 30 kPa 106 kPa Air freight 40 C 55 C 10 96 75 96 30 kPa 106 kPa 8 2 Storage Air temperature Relative humidity Atmospheric pressure In transport packaging 25 C 55 C 10 96 95 96 70 kPa 106 kPa Without transport 5 C 45 C 10 95 70 kPa 106 kPa packaging 8 3 Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community
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