Model 66 & 77 Peristaltic Pump Series User`s Manual
Contents
1. 0eeeeeeeees 28 Declaration Of Contormity seeekeeEeEEREREESEESEREEEEEEEEEEREE el 29 Publication 5387 001 REV D Harvard Apparatus Peristaltic Pump Model 66 77 General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above lim itation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard2. and the communication baud rate must be selected a Press SET and 1 Repeat pressing 1 until CHAIN is displayed press ENTER b Address The display now reads Adr 00 This display represents Address number The colons flash to indicate that a setting is required The numerical keypad is used to enter the address number from 0 to 99 Each pump must be given a different address number When the correct address number is displayed press ENTER amp Baud Rate After pressing ENTER display now reads b 2400 that is baud rate 2400 bits second Repeat presses of 1 toggles through the baud rate selection b 300 b 600 b 1200 b 2400 When the required baud rate is displayed press ENTER The display clears to zero ready for dispensing ENDPOINT SLOWDOWN This feature which slows the pump as the setpoint is approached and can be selected for improved accuracy a Press SET and 2 The display reads E OFF or E ON Each time 2 is pressed the states switch from ON to OFF Press ENTEOR when the required state is displayed ANTI DRIP This feature reverses the pumphead at the completion of a dispense to prevent any unwanted extra drops falling into the dispense container a Press SET and 3 Display reads A ON or A OFF Pressing 3 changes from ON to OFE Press ENTER for displayed function TTL The pump has a nine pin female connector for
3. 4 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continued e 12 4 Communication RS 232C format is No parity 8 data bits 2 stop bits INTERFACE QUERY AND COMMAND REFERENCE All spaces transmitted are ignored Transmissions to a pump terminate with CR Carriage Return Format is address query or command CR Address is Pump address 0 99 Default is 0 a b can be an integer or decimal point Query format address XXX CR XXX 3 letter e g 12 VOL CR Pump 12 what is volume setpoint Command format address VOL CR e g 12 VOL 19 84 CR Pump 12 set volume setpoint at 19 84 ml d Query Commands KEY RUN STP VLA CLA VER RAT VOL E x OK Ok CAL kk OK NUM integer max 99999 INT time ATD on off ENP on off MOD BAT PMP DIS DIR CW CCW REV IN OUT d ON OFF or integer or decimal point e X XX XX hr min sec Publication 5387 001 REV D Return to keyboard Start pump Stop pump Query volume accumulator Clear volume accumulator Query software version Set Query rate Set Query target volume Set Query calibration factor Set Query batch limit Set Query batch wait interval Set Query anti drip Set Query endpoint slowdown Set Query pump mode Set Query pump direction Read TTL pin Output TTL pin d ON 5V OFF OV 4 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continu
4. Apparatus 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device Publication 5387 001 REV D s Harvard Apparatus Peristaltic Pump Model 66 77 General Information Contd This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patients CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS Publication 5387 001 REV D Models 66 77 Specifications Model 66 Model 77 Harvard Apparatus Peristaltic Pump Model 66 77 Accuracy Reproducibility Volume Size Weight Power Leakage to
5. Applicable response will be given If space space NA CR If the interval is invalid an Out of Range response will be given If space space OOR CR eem Prompt character will be Query Interval INT Response is of the following format If d dd dd CR Prompt character will not change ATD ON OFF Request to set or query anti drip setting 1 Set ATD ON turns anti drip function ON ATD OFF turns anti drip function OFF Command will not be accepted if the pump is running Publication 5387 001 REV D R Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continued A Not Applicable response will be given If space space NA CR em Prompt character will be 2 Query ATD If anti drip function is ON response will be If space space ON CR If anti drip function is OFF response will be If space space OFF CR Prompt character will not change ENP ON OFF Request to set or query end point deceleration setting l Set ENP ON turns end point deceleration function ON ENP OFF turns end point deceleration function OFF Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR EI Prompt character will be 2 Query ENP If end point deceleration function is on response will be If space space ON CR If end point deceleration function is off r
6. Model 66 77 Pump Operation Continued To connect the pump a Connect the cable to the computer and pump b If appropriate connect all pumps together in the daisy chain from OUT of pump 1 to IN of pump 2 etc c Tighten retaining screws Transmit Receive GND GND GND GND Transmit Receive Port 2 4 d Port 1 out in Figure 2 RS 232 Connection 12 2 Setting the Baud Rate The RS 232C interface is bidirectional with data transmitted one bit at a time The rate of transmission is the baud rate number of bits per second The pumps operate at 300 600 1200 2400 a Press SET and 1 Continue to press 1 until CHAIN is displayed Press ENTER The display will now read the pump address ADr 01 b Enter the correct address location and ENTER The display will now show the current baud rate setting c Repeat presses of 1 changes the rate b 300 b 600 b 1200 b 2400 When the required rate is displayed press ENTER This procedure must be followed for every pump connected to the computer 12 3 Front Panel Indicator After the pump is configured and the RS 232C connection is made the pump is ready for remote control After receiving the first command to its address the pump keypad footswitch and timer are disabled and the REMOTE light is illuminated Local control is re established using the KEY command Publication 5387 001 REV D
7. is displayed with ml rev illuminated while the CAL key is depressed The dis play defaults to 0 0000 when the CAL key is released To review the new calibration factor press CAL The new value will be dis played for as long as the CAL key is depressed When your finger is removed the display will default to volume again Calibration dispenses should be over 10 ml so that drop size becomes insignificant Publication 5387 001 REV D R Harvard Apparatus Peristaltic Pump Model 66 77 Auto Calibration Auto Calibration When the pump is connected to a weighing scale and the CAL mode is selected the pump will dispense liquid into the container on the scale and the calibration sequence is fully automatic When the RUN key is pressed the scale is tared and the pump displays a set of hor izontal dashes After taring the dispense proceeds The endpoint slow down and anti drip features are automatically selected When the dispense is completed the dispensed weight registered by the scale is read by the pump and used to automatically recalculate the calibration factor The calibration factor is now in units of weight revolution For scale correction see Section 8 2 Publication 5387 001 REV D 5 Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation The pump must now be set for flow rate volume and the operational mode selected 1 2 3 4 FLOW RATE a Press SET and R
8. of weight is controlled by the weight measured by the scale The weight feedback loop provides control independent of the pump action and guarantees very accurate dispenses a Press SET and 1 Press 1 again until SCALE is displayed press ENTER The display should read EtLEr Mettler OHAUS OHAUS SArTr Sartorius Repeat presses of 1 changes the display until the appropriate name is displayed Then press ENTER The display clears back to zero The weighing scale must also be configured METTLER PM Series Baud rate 2400 Parity even Pause 0 OHAUS GT Series Baud rate 2400 Auto print feature OFF Stable data only OFF Serial data frame Fr 7 SARTORIUS Basic Series E1200S E2000D MP8 4 series Code Settings 211 External print command without stability 225 2400 baud 234 Even parity 432 Beeper off 311 Weight only models with PLUS performance package Publication 5387 001 REV D gt Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continued 10 11 8 3 Daisy Chain Multiple pumps can be connected through a daisy chain and controlled by a single PC The PC is connected to the IN connector of the first pump and the OUT of the first pump is connected to the IN of the second pump Daisy chain is repeated to connect all pumps After the connections are made each pump must be given an identifying address
9. 11 12 such as Max Distance L COM P N RBAO98F 15M Front View Computer Connection Terminal Port Setti Chart ettings g RJ12 6x6 RJ11 RJ12 STD DB9 Baud Rate 9600 2 RD or match pump baud rate 3 TO Data Bits 8 5 Sig ground St its op Bits 2 GND View of RJ11 6x4 Adapter View of Cable Harvard Apparatus Peristaltic Pump Model 66 77 Publication 5387 001 REV D Declaration of Conformity Application of Council Directive s Standard s to which conformity is declared Manufacturer s Name Manufacturer s Address Type of Equipment Model No 89 336 EEC EN55022 1994 CISPR22 1993 IEC 1000 4 3 1995 IEC 1000 4 2 1995 Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 U S A Peristaltic Pump Model 66 77 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Date CE HARVARD APPARATUS United States of America January 25 1996 7 S Signature Beth Bauman Full Name VP Engineering Operations Position
10. 5 6 7 8 9 10 Load the pump with the required tubing and liquid to be pumped Prime the pump by pressing RUN The pump is initially configured to run until stopped with the STOP key Press SET and CAL to select the Calibration mode ml rev will be illumi nated and flashing Enter the approximate calibration factor from the table below using the numerical keypad When the correct number is displayed press ENTER Approximate calibration factors Tubing ID inch 1 4 5 85 N A 1 8 1 22 0 88 1 16 N A 0 22 Tubing wall thickness is 1 16 inch The pump should be run in the Calibration mode for a more accurate cali bration factor The pump can be operated at the preset rate and volume set tings or more appropriate RATE and VOLUME settings can be entered after reading Pump Operation Press SET and CAL to select Calibration mode Press RUN The pump will start the display will show the increasing volume pumped The volume displayed is calculated based on the programmed cali bration factor and may not be accurate When the dispense is completed the pump stops automatically The Rur light is shut off and ml is flashing Measure accurately the dispensed volume Enter the dispensed volume using the numerical keypad The display will show the entered numbers When the correct value is displayed press ENTER The pump will now automatically recalculate the calibration factor which
11. 7V1 0 CR IN d Request to read in the TTL logic value at the specified pin d on the Harvard Apparatus Peristaltic Pump Model 66 77 external 9 pin D SUB connector Valid pin numbers for input are 6 7 8 and 9 If the pin specified is valid and if the pin level is high response will be If space space ON CR If the pin level is low response will be If space space OFF CR If the pin specified is invalid an Out of Range response will be given If space space OOR CR Prompt character will not change Publication 5387 001 REV D Pump Operation Continued R OUT d lt ON OFF gt Request to set the TTL logic value at the specified pin on the external 9 pin D SUB connector Valid pin numbers for output are 2 3 and 4 If the pin specified is valid no response is given If the pin specified is invalid an Out of Range response will be given If space space OOR CR Prompt character will not change Harvard Apparatus Peristaltic Pump Model 66 77 Publication 5387 001 REV D J Harvard Apparatus Peristaltic Pump Model 66 77 Table 1 RS 232C Commands command gt lt adr gt lt cmd gt lt CR gt adr gt ad cmd S KEY RUN STP VLA CLA RAT lt float gt VOL lt float gt CAL lt float gt NUM lt integer gt INT lt time gt ATD ONIOFF ENP ONIOFF MOD BATIPMPIDIS DIR CWICCWIREV VER IN d OUT d lt ONIOFF gt Publ
12. ATE The ml min LED will flash indicating that the value with these units is ready for updating b The flow rate value in units of ml minute is entered from the numerical keypad As the numbers are entered they will be displayed c When the correct value is displayed press ENTER VOLUME SETTING a Press SET and VOLUME ml unit indicator will flash b Enter value of volume to be dispensed c Press ENTER Display defaults to zero SELECT PUMPING MODE a Selection is made by pressing MODE The present mode selection is displayed as either Pump Dispense or Batch b Press the MODE key until the required mode is displayed PUMP MODE a Continuous pumping Change flow rate if required b Dress RUN STOP to commence Display shows increasing pumped volume ml units c To pause press the RUN STOP key Press RUN STOP key again to restart The pump continues and displays the accumulated volume DISPENSE MODE Pump runs at set rate to a set volume and automatically stops a Use SET RATE and SET VOLUME keys for setpoint entries BATCH MODE This mode is for repetitive dispenses of a set volume The number of dispenses and time delay interval between dispenses can be set 6 1 Number of Dispenses a Press SET and NUMBER Display shows the last setting Use numerical keypad to enter required number of dispenses b When correct number is displayed press ENTER Display clears rea
13. Ground Ground Resistance Drive Motor Flow Rate Minimum Maximum Step Rate Minimum Maximum Maximum Back Pressure Interface Selectable Baud Rates Publication 5387 001 REV D 1 1 Adjustable from 4 to 100 cc stroke 229 x 206 x 89 mm 9 x 8 1 8 x 3 1 2 in 3 6 kg 7 9 Ib 115 230 volts 50 60 Hz via internal selector switch 30 W Fuse 0 5A S B Typically lt 20 Typically lt 0 5 O Microprocessor controlled stepping motor 0 01 ml min 200 ml min 27 3 sec step 416 7 usec step 30 psi Bidirectional RS 232C 300 600 1200 2400 1 1 Adjustable from 4 to 100 cc stroke 241 x 206 x 127 mm 9 1 8 x 8 1 8 x 5 in 5 1 kg 11 1 4 Ib 115 230 volts 50 60 Hz via internal selector switch 30 W Fuse 0 5A S B Typically lt 20 Typically lt 0 5 Q Microprocessor controlled stepping motor 0 01 ml min 1 8 in ID 700 ml min 1 8 in ID 27 3 sec step 416 7 usec step 30 psi Bidirectional RS 232C 300 600 1200 2400 s Harvard Apparatus Peristaltic Pump Model 66 77 General Description The Harvard Model 77 and Model 66 are microprocessor controlled stepper motor driven peristaltic pumps The volume dispensed is proportional to the pumphead rotation and to the num ber of steps moved by the stepper motor The microprocessor maintains count of the number of steps and the motor can be microstepped with up to 6400 microsteps per revolution of the pumphead T
14. Model 66 amp 77 Peristaltic Pump Series User s Manual Model 66 Small Peristaltic MAI 55 7766 Model 77 Large Peristaltic MAI 55 7777 HARVARD APPARATUS Publication 5387 001 REV D WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application o
15. R Display reads b baud e Dress 1 until desired baud rate is shown then ENTER Display now reads 0 0000 Remote Control When a pump is sent a command or a request is made for its prompt it enters remote mode indicated by the remote light on the pump s display During remote mode the KEYBOARD FOOTSWITCH AND TIMER ARE DIS PLAYED The keyboard footswitch and timer can only be re enabled by either sending a KEY command see below or turning the power to the pump OFF and then ON again A pump will not accept pump chain communication while it is in a setting mode entered when user presses the SET key or during calibration Any settings changed from remote mode will not be stored in the pump s non volatile memory When remote mode is exited via the KEY command any changes to the settings of the pump will cause all settings including those changed during remote mode to be stored in non volatile memory Prompts A prompt is a string of ASCII characters sent by a pump 1 f line feed 1 or 2 digit address prompt character prompt characters spump stopped gt pump running pump paused or stalled pump between batches Commands Each command sent to the pump chain is a string of ASCII characters with the following formats and meanings all spaces ignored by pump Harvard Apparatus Peristaltic Pump Model 66 77 a CR Stop all pumps b pump address CR Request for prompt The pump wi
16. TTL input outputs Figure 1 11 1 Input a Momentary contact closure eg footswitch Momentary closure between pin 1 ground amp pin 6 causes the pump to change state Publication 5387 001 REV D R Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continued that is from RUN to STOP or from STOP to RUN b Contact ground eg timer or relay Circuit from pin 7 to ground causes the pump to RUN STOP Rising edge Pump runs Falling edge Pump stops c Pins 6 7 8 and 9 are software readable NOTE Footswitch and timer are inactive during remote operation 11 2 Output a Pin 1 is ground Pins 2 3 4 are software controlled ON 5V OFF 0V Pin5 5V 12 RS 232 INTERFACE The peristaltic pumps Models 77 and 66 use an RS 232C interface to communicate with a computer The same communication system is used by most terminals modems and many laboratory instruments REMA Foot Switch D Timer Figure 1 User I O Connector Specifications 12 1 Computer to Pump Connection Any device with RS 232 communication such as a PC has a serial or COM port usually 25 or 9 pin male connectors A special cable fitted with a 9 pin female connector is used to connect the serial port to the pump which is equipped with a telephone socket marked RS 232 IN The cable wiring diagram is shown below in Figure 2 Publication 5387 001 REV D R Harvard Apparatus Peristaltic Pump
17. V D 4 Harvard Apparatus Peristaltic Pump Model 66 77 General Description Contd RS 232C Interface Remote computer control daisy chain dispense by weight interface with other laboratory equipment TTL RUN STOP controls through hand or footswitch or contact closures 5V signal to activate a relay TTL signal level input Non volatile memory The last settings are stored in memory Momentary loss of power The pump stops and displays flashes to alert the user The pump is designed so that the illuminated display will always indicate the sta tus of the pump and the appropriate units associated with the pump operation For example when dispensing the display shows the increasing volume with units of ml and run illuminated When the dispense is completed and the pump stops the run is switched off Publication 5387 001 REV D Harvard Apparatus Peristaltic Pump Model 66 77 Initial Set Up 1 Read the manual N Ed Load tubing in pumphead Select the correct tubing and locate the tubing in the appropriate size tubing holder machined in the pump block Feed the tub ing around the pumphead by threading the tubing through the round scallop in the pumphead Manually rotate the pumphead and simultaneously feed the tubing around the moving pumphead through the scallop Fasten the free tubing into the second tubing holder The tubing should be snug around the pumphead but not too
18. ccumulator If the pump was paused it will return to its stopped state If pump is running a Not Applicable response will be given If space space NA CR eem No response Prompt character will be RAT lt float gt Request to set or query rate setting 1 Set rate RAT rate Rate is of format HIT If rate is accepted and valid rate will become the new rate Rate will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR If the rate is invalid an Out of Range response will be given lf space space OOR CR EI Prompt character will be Publication 5387 001 REV D Pump Operation Continued 2 Harvard Apparatus Peristaltic Pump Model 66 77 Query rate RAT Response is of the following format lf fF CR Prompt character will not change VOL lt float gt Request to set or query volume setting 1 Set volume VOL volume Volume is of format fF If volume is accepted and valid volume will become the new volume Volume will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR If the volume is invalid an Out of Range response will be given If space space OOR CR EI Prompt character will be Query volume VOL Response is of the following format lf Om CR Prompt character will not change CAL lt float gt Request to set
19. dy for dispense c Continuous Dispensing If NUMBER is set to 0 the pump interprets this as an infinite number of dispenses and continues to dispense until manually stopped Publication 5387 001 REV D Pump Operation Continued R 6 2 Time Interval Between Dispenses a Press Set INTERVAL Display reads 0 00 00 representing time interval in Hours Minutes Seconds The colons will flash to indicate ready for setting b The interval is entered from the numerical keypad and displayed as entered These numbers are displayed from the right For example For interval 1 hour 15 minutes 23 seconds enter 11523 For interval 49 seconds enter 49 Minimum time interval is 1 second c When the correct value is displayed press ENTER d Press RUN to start Display reads volume and automatically stops at the setpoint The display changes to show the number of completed dispenses After one second the display changes again to time interval counting up in seconds When the interval setpoint is reached the pump automatically moves on to the next dispense e Ifthe RUN STOP key is pressed during the time interval the RUN STOP does not act as a pause but stops the batch dispense mode and resets the batch number to zero 7 RS 232C INTERFACE The RS 232C Interface Connectors are telephone jacks mounted on the rear panel and marked IN and OUT Through the RS 232C Interface the pump can be controlled remotely by a PC or can com
20. ed 14 15 Interface Responses The pump responds back to the PC after each query or command pump output a response prompt prompt If adr pump stopped If adr gt pump running CW If adr pump stopped interval between dispenses If adr t pump stalled or paused If line feed adr address of pump 0 99 response If message CR message bb bb NA not available bb OOR out of range bb text integer float time text any string of ASCII characters integer ddddd d 0 9 float FERE f integer or decimal point time d dd dd hr min sec b blank If ASCII 10 line feed CR ASCII 13 carriage return MAINTENANCE No maintenance is required except for general cleaning with water and mild detergent RS 232C PUMP CHAIN COMMUNICATION Configuration of Pump Chain Each pump in the pump chain needs to be individually configured as a mem ber of the pump chain To configure a pump two items of information are needed baud rate of the host and address of the pump Allowable baud rates are 300 600 1200 or 2400 One baud rate is selected for the entire chain to use Each pump in the chain is assigned a unique address from 0 to 99 Enter the configuration into the pump as follows e Dress keys SET 1 and continue to press 1 until display is shown as SCALE e Dress ENTER Display reads ADR nn Publication 5387 001 REV D Pump Operation Continued R e Enter 1 or 2 digit address then ENTE
21. esponse will be If space space OFF CR Prompt character will not change MOD BAT PMP DIS Request to set or query pumping mode set MOD BAT puts pump in batch mode MOD DIS puts pump in dispensing mode MOD PMP puts pump in pumping mode Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR em Prompt character will be Query MOD If pumping mode is batch response will be If space space BATCH CR Publication 5387 001 REV D Pump Operation Continued R If pumping mode is dispense response will be If space space DISPENSE CR If pumping mode is pump response will be If space space PUMP CR DIR CW CCW REV Request to set or query pumping direction set DIR CW sets pumping direction to clockwise DIR CCW sets pumping direction to counter clockwise DIR REV reverses direction of current pumping direction Command will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR eem Prompt character will be Query MOD If pump direction is clockwise response will be If space space CW CR If pump direction is counter clockwise response will be If space space CCW CR Prompt character will not change VER Request for version of pumps embedded software Response for the present version will be If space space 7
22. f the RoHS Directive to its products and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste e Special Collection Disposal Required 5 Harvard Apparatus Peristaltic Pump Model 66 77 Table of Contents SUBJECT PAGE NO General Information Warranty and Repairs 2 3 Technical Specifications ccccccecceeesesseeeeeeeeeeeeeeeeesenseneeees 4 General Description ccscesesseeeteeeeee eee eeeeeeeseneeneeeeeeeeeees 5 6 Initial Set Up eege dene eset EE ee 7 8 Calibration iccccesccescesscetec die doves ENEE EEN ENEE 9 10 Pump Operation le 11 Volume Setting oo eeeeecesceeceeeeeeeeeeeeeeeeeeeaeseeeeeeseeaeeeeaaeeneaes 11 Select Pumping Mode 1 0 cece ceeeeceeeeeeeeeeeceeeeeetaeeeteneees 11 Pump ModE 4211s ciate aaestat ania 11 Dispense Mode cccceeeeccceeeeeceeeeeeeeneneeeeeenseneeeeeeneneees 11 Batch Mode delen ae aaaea aa adan d 11 12 RS 232 Interface 0 ccccccceceesseececeseneeeeeeeeeaeeeeseestaeeesseeaees 12 Printer Weighing Scale or Daisy Chain Selection 12 14 Endpoint Glowdown 14 Anti Drip EE 14 RRE 14 15 RS 232 Interface Connections sssessesereeseeeereeee n 15 17 Interface Query and Command Heierence 17 18 Maintenance sees EEN dee ees eee 18 RS 232C Pump Chain Communications ssaaeeaaaeae 18 25 Table 1 RS 232C CommandS ccecteeeeeeeeeeeeeeeeeee 26 27 Appendix A Pump to PC Connection
23. his fine control permits great dis pensing precision both of flowrate and volume dispensed In addition to compen sate for variations in tubing and flow of different liquids a simple to use calibration method is built in which will automatically recalculate the volume dispensed per revolution thereby ensuring greater accuracy Microprocessor control allows many options to be included in the pump such as volume dispense and repetitive batch dispenses Via RS 232C the pump can be interfaced with many laboratory devices and up to 100 pumps can be controlled by one PC through a daisy chain system included in the pump The digital communication interface can be used to connect the pump directly to a weighing scale to establish a feedback loop and dispense by weight A TTL input output is also included Features Pump Mode Pump runs continuously at set flow rate Dispense Mode Pump runs at set flow rate to the set volume Batch Mode Multiple dispenses at set flow rate and set volume The number of dispenses and the time delay interval between dispenses can be selected Calibration Mode Used to improve dispense accuracy Precise measure ment of volume or weight dispensed per pumphead revolution Endpoint Slowdown The pump slows as the setpoint is neared for improved dispense accuracy Anti drip Ar the completion of a dispense the pump is reversed to prevent any additional unwanted drops Publication 5387 001 RE
24. ication 5387 001 REV D return to keyboard start pump stop pump query volume accumulator clear volume accumulator set query rate set query target volume set query calibration set query batch limit set query batch wait interval set query anti drip set query end point dec set query pumping mode set query pumping direction query software version read TTL pin d output TTL pin d 4 Harvard Apparatus Peristaltic Pump Model 66 77 Table 1 RS 232C Commands Contd All spaces ignored Maximum 5 digits per integer float or time output gt lt response gt lt prompt gt response gt lt response gt lt response gt lt lf gt lt message gt lt cr gt prompt lt f gt stopped lt lf gt gt Dumping lt lf gt between batches lt J paused or stalled message gt bb unrecognized command bb NA Com not presently applicable bb OOR entered value out of range bb lt text gt lt integer gt lt float gt lt time gt float gt ffffff f gt dl integer gt ddddd time gt d dd dd d gt 01112314 1516171819 b gt space cr gt ascii 13 carriage return If gt ascii 10 line feed text gt any string of ascii characters legend IT optional capital terminals lower case non terminals either or Publication 5387 001 REV D Appendix A Pump to PC Connection ro vo Cop C R Deiere DB9 Female to Computer Pump Pinning 4 6 PIN RJ
25. municate with other laboratory devices such as a weighing scale Multiple pumps can be controlled by a single PC through a daisy chain inter connection between pumps The IN connector is used to connect the pump directly to a PC or other devices The OUT connector is used in the daisy chain and is connected to the IN connector of the next pump in the chain Harvard Apparatus Peristaltic Pump Model 66 77 8 PRINTER WEIGHING SCALE OR DAISY CHAIN SELECTION After the printer scale or daisy chained pump connection is made the pump must be configured to communicate to the connected device Configuration selection is made by pressing SET and 1 Repeat presses of 1 changes the display through Print Scale and Chain When the appropriate display is recognized selection is made by pressing the ENTER key Publication 5387 001 REV D R Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continued 8 1 8 2 Printer a Press SET and 1 Repeat pressing key 1 until Print is displayed Press ENTER b Printer is connected to the IN connector then the pump communications interface is set c Printer Configuration Baud rate 2400 Word size 8 Parity none Stop bits 2 Weighing Scale The pump is programmed to pump by weight when connected to a weighing scale A weight feedback loop is established automatically and the dispense which is in units
26. or query calibration setting 1 Set calibration CAL calibration Volume is of format fF If calibration is accepted and valid calibration will become the new calibration Calibration will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR If the calibration is invalid and Out of Range response will be given If space space OOR CR EI Prompt character will be Query calibration CAL Response is of the following format lf Om CR Prompt character will not change Publication 5387 001 REV D Pump Operation Continued R NUM lt integer gt Request to set or query batch limit number Harvard Apparatus Peristaltic Pump Model 66 77 1 Set number NUM number Number is of format ddddd If number is accepted and valid number will become the new number Number will not be accepted if the pump is running A Not Applicable response will be given If space space NA CR EI Prompt character will be Query rate NUM Response is of the following format If ddddd CR Leading zeros will be replaced by spaces Prompt character will not change INT lt time gt Request to set or query batch interval time 1 Set Interval INT interval Interval is of format d dd dd hr min sec If interval is accepted and valid interval will become the new interval Interval will not be accepted if the pump is running A Not
27. th the indicated address responds with its prompt c Optional pump address command CR Send command The pump with the indicated address executes the command then responds with its prompt The optional pump address if not specified will default to pump address 0 The following are the allowable commands and their responses during execu tion After each command is executed the addressed pump sends its prompt If a command is not recognized by an addressed pump the pump will respond with the following lf space space CR Publication 5387 001 REV D J Harvard Apparatus Peristaltic Pump Model 66 77 Pump Operation Continued KEY Leaves remote mode turning remote light off and re enabling keyboard footswitch and timer No response Prompt character not changed RUN Starts pumping according to the present setting of the pump If pump is already pumping command has no effect No response Prompt character changed to gt STP Stops pump if it was running If pump is already stopped command has no effect No response Prompt character changed as follows e mm If pump was running prompt gt new prompt changed to paused If pump was executing a batch interval prompt new prompt changed to zem stopped VLA Request for volume accumulator Response is of the following format lf Om CR Prompt character not changed CLA Request to zero volume a
28. tight Rotate the pumphead manually a few revolutions to ensure free movement Model 77 Model 66 AB we O Tubing Size0 Tubing Size0 A 1 4 ID B 1 8 ID A 1 8 ID B 1 16 ID 3 Tubing recommendations Norprene Tygon R 1000 C flex Viton Silicone ID 1 16 1 8 1 4 inch wall thickness 1 16 inch Registered trademark Norton Co Norton Co Concept Inc E I duPont de Nemours and Co 4 Turn on the main power switch located on the rear panel above the fuse hold er The display should initially illuminate all LED s followed by 0 0000 flash ing to indicate the pump is ready Press any key to stop the flashing and make ready for entries Publication 5387 001 REV D R Harvard Apparatus Peristaltic Pump Model 66 77 Initial Set Up Contd Vi we Note that U S models are set for 115 VAC Overseas models should have an appropriate plug installed and the voltage selector switch inside the pump will be correctly adjusted Polarity for international line cord Brown high Blue neutral Green ground Dy we The pump is delivered with a programmed calibration factor corresponding to 1 8 inch ID tubing For accurate dispenses the pump should be calibrated to match the users conditions Publication 5387 001 REV D s Harvard Apparatus Peristaltic Pump Model 66 77 Calibration Calibration 1 2 3 4
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