Cell Counter - EWH BMET Library
Contents
1. P openings hinges and connecting or interacting pieces are clean See BTA skills for Cleaning Check for dents scratches and other major damage to the Is the device free of external j i 4 outside of the device In particular look to see if there is damages evidence of whether or not the device has been dropped Damage to the exterior of the device can indicate the presence of internal damage and offer insight as to where inside the 5 Assess severity of damage casing to begin looking for damage If damage to the exterior is minimal it can likely be ignored If too severe it is possible the device will not function at all Using appropriate BTA skills on Casing Attachment and 6 Repair as applicable 8 pprop A 8 i Cleaning repair scratches dents etc as possible Itis a possible user error that the device is simply missing key components that are involved in its use but are not inherently Make sure you have all necessary re i present within the device If possible check the user manual or 7 reagents solutions and trouble i hoatn tols any other type of documentation to determine whether or not S G the appropriate auxiliary components are being used and being used properly The measurements that this device makes are minute and Is the device on a clean sturdy aha 8 precision is of the utmost importance A sturdy and level level surface surface is necessary to ensure accurate results A clean sturd2. guide the troubleshooting process 22 Follow on screen troubleshooting If it is built into the software the device will guide through the instructions steps to take to approach problems associated with the malfunctioning part of the machine 23 Run control cycle Control cycles are run with deionized water The system is set to run as it would normally with a sample but only deionized water is used for the sample and each of the reagents This enables the observation of all of the moving parts within the device and also shows whether or not the device is functioning mechanically even if it does not display that proper results or values 24 Follow PM procedure See accompanied PM Preventative Maintenance procedure 25 Device is ready Device should be functioning properly 26 Run atest cycle A test cycle is the same as a control cycle See explanation for Box 23 27 Does the test run to completion The test cycle has run to completion if the cycle drew the water through all of the appropriate channels was not stopped along the way produced no error messages and displayed results at the end 28 Does the probe or aperture appear damaged The sample probe is a thin metal rod with a small opening like a needle that is open to the exterior of the device It is used to draw up the sample into the device to initiate a run cycle A damaged probe is likely to be b
3. Cell Counter Flowchart 17 Open device casing Check for loose worn out wires and corrupt circuitboards is the device free from debris Clean exterior of device 3 Assess severity of damage 5 Repair as applicable 6 Make sure you have all necessary reagents solutions and trouble shooting I tools 5 the device on 2 clean sturdy level surface surface Move device toa clean sturdy level Move device to a room within appropriate temperature range or adjust temperature of current room 41 16 T4 Move device away Power device on from strong EM devices 43 Troubleshoot power supply turn on 24 Follow PM procedure his Set up the device to run as normal show an error message N Run a test cycle N it possible follow on screen instructions to oubleshoo 33 Go to box 49 output the propriate valugg Y Problem is a user or sample 22 34 eror 23 Follow on screen Run control cycle troubleshooting instructions ts Check for clogged tubing 41 Skip to box 45 Check connections to waste system reservoirs reagent 42 supplies etc Continue to Coulter 4 7 Check piston Counter Chart Part pressure and seals 2 45 Close casing i i i 4 31 Manually adjust probe to home position Kithe probe aperture clogged Undog 37 Open casing fan fluid flo
4. Y flowchart for assistance Inspect the way that the casing is put together and use BTA Open device casing Check for P y 515P 8 j skills for Casing to determine the order that the pieces need to 17 loose worn out wires and corrupt are be removed Inspect for worn out frayed rusted etc wires circuit boards i i Use BTA skills Electrical Simple to repair as necessary Press the keypad or screen if touchscreen or appropriate buttons to prepare device as if to run a cycle Determine 18 Does the screen respond to the whether or not the screen is responding as it should Possible appropriate input examples of incorrect screen inputs include moving to the incorrect menu one that does not correspond to the input pressed a frozen screen an error message etc Set all system modes and settings as if a sample were to be run normally This verifies that the device is being used proper 19 Set up the device to run as normal y 8 propery and that as troubleshooting continues user error is not a factor Error messages are fairly obvious as they are accompanied by 20 Does the screen show an error the words caution error warning etc Error messages message serve as an internal way for the device to give a better indication of which area of the device is malfunctioning 21 Is it possible to follow on screen With higher level and more modern models the device may instructions to troubleshoot have software designed to
5. ater See BTA skills 63 Clean electrodes on Switches and Connectors When opening casing be sure to keep track of all panels and 64 Close casing screws Close the casing in the exact reverse of opening the casing See BTA skills on Casing 65 Device is ready
6. d 32 Does the device output the appropriate values Look at the display screen to verify that the software of the machine is functioning Specific values vary by model and brand the main objective of this step is to make sure that the electronic components of the device are functional if not necessarily accurate 33 Go to box 49 34 Problem is a user or sample error Each of the components of the device are functioning properly 35 Replace probe if possible If not possible the device cannot function 36 Unclog Probe may be unclogged simply by soaking and washing with warm deionized water If necessary it may also be unclogged using a small needle or brush depending on the size of the probe and its opening Aperture can be unclogged using a small needle or brush but must be done gently so as not to damage the device See BTA skills on Blockages 37 Open casing If it hasn t been done yet open the casing as described in step 16 Opening the casing not only allows for inspection of the electronic elements but also provides an opportunity to inspect the basic physical and mechanical workings of the device See BTA skills on Casing 38 Can fluid flow through tubing easily Indicators of fluid not flowing easily are bubbles leaks or the machine stopping See BTA skills on Blockages 39 Check for clogged tubing Tubing may be clogged simply by having folded in on it
7. ent or chipped The aperture is inside the device and is sized on the order of millimeters A damaged aperture is likely to be cracked or corroded 29 Is the probe or aperture clogged As the probe serves to draw fluid up and into the device and as the aperture facilitates fluid flow through the device it is possible that debris may build up causing either to clog and thereby inhibit the analysis of the sample See BTA Skills on Blockages 30 Does the probe rest in its neutral home position The neutral home position of the probe should be marked either by a tick mark or a line on the casing or on the probe itself If this mark exists make sure that the probe is aligned properly so that it is functioning at maximum capacity If the device does not have a marking to indicate the neutral home position place an empty sample collection container in the appropriate position as if the device were to run a test and verify that the probe is in a position so that it can draw up the entirety of the contents of the sample collection container 31 Manually adjust probe to home position It is possible that the probe may be adjusted or calibrated via instructions and a process through the device software on the screen See BTA skills on Calibration If this is the case follow these instructions Otherwise it is likely that the probe is held in position by a series of set screws that can be manually adjuste
8. ls for Lighting Indicators 57 Does voltage drop across socket correspond to bulb specifications If the lamp has a bulb component it is likely to have printed specifications as to the current and voltage that correspond to the optimal functionality of the bulb Use a multimeter to check the voltage See BTA skills for Lighting Indicators 58 Check wires and connections to The simplest way that the lamp component may fail other socket than a broken or worn out bulb is in the immediate circuitry connecting the lamp to the power supply See BTA skills for Lighting Indicators If the socket is receiving the appropriate current and voltage 59 Replace bulb the problem lies within the bulb which should be replaced See BTA skills for Lighting Indicators More modern models typically have software menus to guide through the lamp alignment process If not the lamp can be 60 Wein epaien more roughly aligned by Veriiying hat the accompanying photosensors are responding appropriately As with many of the moving parts of the device the lamp can be adjusted manually after loosening the appropriate set screws Are all internal resistors and i ae ons Use a multimeter to check that there is a voltage drop across 61 driving electrodes receiving and current is running through the key electric components current 62 Device is beyond repair Electrodes can be cleaned with deionized w
9. on probably needs to be replaced See BTA skills on Seals and Motors 45 Close casing When opening casing be sure to keep track of all panels and screws Close the casing in the exact reverse of opening the casing See BTA skills on Casing 46 Do all moving pieces operate smoothly As the control test cycle runs verify that pieces that are supposed to be moving are doing so properly The device can break down is via the wear and tear of basic mechanical components Although it cannot be predicted which pieces exactly are of critical loading visually inspecting the system as it runs can verify whether or not there is anything grinding catching in need of lubrication broken blocked caught etc See BTA skills on Mechanical and Motors 47 Are all pieces aligned properly It is possible especially if there is any evidence of external damage or dents the device is not functioning properly because the moving parts are misaligned 48 Align any misaligned pieces Most pieces or parts are held in place by set screws or hex screws that can be loosened to allow for the adjustment of the piece As a general rule all interacting parts with a round geometry should align concentrically and all translational parts should move in straight perpendicular lines 49 Do the temperatures of the baths match the indication on the attached cables For most models the desired temperature range for the baths or
10. reservoirs of each of the liquids or reagents used in a run cycle will be marked on the small cable connecting the hot plate or similar mechanism that maintains the temperature to the power supply Use a thermometer to verify that the temperature of the specific liquid falls within the appropriate range However if the temperature does not match properly very few models have associated processes to fix the problem 50 Empty clean and refill baths Reagents in device should be discarded appropriately The device can be cleaned using deionized water only and running a test or if the model has one a cleaning cycle Baths can then be refilled It is possible that temperature and even general device running issues stem from build up in the tubing reservoirs and baths 51 Check for broken gears bands or parts As the control cycle runs visually check for any mechanical load bearing or linking parts that may have broken Fix these as possible 52 Check for blocked pathways As parts move within the device it is possible especially if there is evidence of external damage that pieces or parts within the device have shifted or been knocked out of place In addition to throwing off alignment this can manifest itself as the blockage of the pathway of a moving part Any blockages should be cleared and if any of the blockages involve broken or misplaced parts these should be reattached or replaced a
11. s appropriate 53 Are the motors functioning properly Although not the case for all models of the device many power the movement and coordination of internal parts and pieces with motors To ensure that the device is working properly check to see if the motors are functioning properly A properly functioning motor will make a small continuous whirring sound when operating and will lead to the motion of another piece or part within the device See BTA skills on Motors 54 Troubleshoot motors Motors commonly fail in two ways mechanically and electrically Motors can wear out or jam or their rotor may be blocked Electrically the wiring in and around the motor can be checked as well See BTA skills on Motors and Electrical Simple 55 Is internal lamp functional In addition to a resistance or voltage based aperture measurement many models especially the more modern models have a lamp or light device of some kind that is used in conjunction with photosensors to take various measurements from a sample To check if the lamp is functional first verify whether or not the lamp is on when the device is on It may also be possible to use the software menus to turn the lamp on or off A lamp is deemed functional if it emits light under the appropriate conditions 56 Remove bulb and check voltage drop of socket Use potentiometer to verify that the lamp component is receiving current See BTA skil
12. self or having been pinched to prevent flow It may also be clogged by debris or build up Any cause of clogging should be removed if possible by removing and rinsing the tubing or using a small brush if the clog is near the opening If necessary tubing can also be replaced See BTA skills on Blockages 40 Are there any leaks Leaks can stem from defective tubing broken valves broken reservoirs or any of the connections between these parts Clamps are also a suitable solution to this problem See BTA skills on Leaking and Clamps 41 Skip to box 45 42 Check valve functionality An alternative and more specific source of leaks may be the valves Valves or valve parts that wear down like O rings can wear down and lose their water tight properties 43 Check connections to waste system reservoirs reagent supplies etc Leaks will often stem not from a particular part but the places where parts intersect and interact These can be replaced tightened or patched up accordingly See BTA Skills on Connections and Seals 44 Check piston pressure and seals Not every model of the device will contain pistons however many do and the purpose of the pistons is to draw up and transport solution to different parts and processes within the device It is imperative that the piston is actually drawing up liquid If not the seals can be adjusted and tightened If the problem persists the pist
13. through tubing easily Ny 32 Go to box 49 output the propriate valug Problem is a 40 user or sample 34 error Are there any leaks 2 Check valve functionality Continued from Coulter Counter Chart Part 1 Run a test cycle Do all moving f ieced 48 pieces operate smoothly Align any 37 J pe N r T geans pathways g parts 52 properly Troubleshoot 54 motors 51 Run a test cycle Problem is a user or sample 32 error D6 the temperaturesf the baths match the indication on the attached cables Empty clean and 50 refill baths Check wires and connections to socket Remove bulb and Does voltage drop acros socket correspond to bulb specifications check voltage drop sg Of socket functional 58 59 Replace bulb 60 Verify lamp alignement Are all internal resistors and driving electrodes receiving current 62 Device is beyond repair Close casing 25 Device is ready Description Text box Explanation or comment 1 Bevi Start troubleshooting process for a work order on a Cell or egin 8 Coulter Counter 2 Is the device free from debris Check exterior of device for any dirt dust or other debris Often parts appear broken or malfunctioning when they are simply not clean It is most important to ensure that all 3 Clean exterior of device py i
14. y level surface is one that does not wobble Move device to a clean sturdy a i DA 9 visibly or tangibly when the device is operated is not on a level surface sa i slant and is wiped free from dust and debris i Use a thermometer to check 16 34 C is the optimal operating Is the atmospheric temperature RAR 10 temperature for both the electronic and liquid components of between 16 34 C the device 11 Move device to a room with Execute as possible appropriate temperature range or adjust temperature of current room Is device near any EM field EM Electro Magnetic fields are generated by very powerful devices such as x ray machines scanners anything with a 12 inducing device radar etc These fields can interfere with the circuitry and the accuracy of device measurements M evi f g EM The simplest solution to this problem is to move the device ove device away from stron 13 dea y p which though bulky is likely much more portable than any of evices the EM field generating devices 14 Power device on Press the on or power button or switch Look for visible signs that the device has turned on Indicators could be sounds of pieces within the device loading up and 15 Does the device turn on i p i 8 up moving a screen lighting up an LED typically green illuminating etc Consult BTA skills on Power Supply and Power Suppl 16 Troubleshoot power supply PPY PP
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