Retiga 3000 User Manual
Contents
1. EE EE ETE EE 13 Resolving Problems with the CaM efa 15 Unresolved Problems Contacting Qlmaging Support 14 SPECIFICATIONS 15 Camera Dimensions Front csset n n KU 15 Camera Dimensions TUTTA 15 Additional Camera Measurements eI 15 Connectors on Back of 16 Power Supply Specifications L a n 16 Focal Plane 17 Sensor Specifications PR en m 17 Sensor Orientation T 18 VO Connector Pinout ERE TTD P M 18 APPENDIX A USB Information 20 USB 2 0 Chipset Incompatibility Notice n r nn s 20 Determining if a USB port is on a Root Hub 21 Www qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 ZH 5 Retiga 3000 USER MANUAL CHAPTER INTRODUCTION The Retiga 3000 is the ideal camera for high resolution snapshot fluorescence documentation With 2 8 m2. Es REI DA BE SMS b Retiga 3000 USER MANUAL QIMAGING LIMITED WARRANTY Standard Product Warranty Plan A Standard Product Warranty Plan is included with every Qlmaging camera purchase This Warranty Plan includes parts and labor for two Tull years starting trom the shipping date of the camera The Standard Product Warranty Plan is provided on all new and used equipment including retired demonstration cameras Extended Product Warranty Plan Extended Product Warranty Plans are conveniently priced and very easy to purchase Available for all Qlmaging cameras the Extended Product Warranty Plan includes parts and labor and is available in one year increments up to five years When you purchase an Extended Product Warranty Plan from Qlmaging you are assured of our commitment to minimizing down times Your needs are our top priority and Qlmaging responds immediately The Qlmaging Service and Support team is focused on expediting all customer requests to provide a fast and complete solution Qlmaging also offers on site training as well as online operational training programs These programs are designed to get you up and running with your new camera quickly and efficiently Contact a Qlmaging Representative to learn more about Extended Product Warranty Plan options WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 q Retiga 3000 USER MANUAL
3. RETIGA 3000 CERTIFICATE OF CONFORMITY CERTIFICATE f b of Conformity TUY Rheinland EMC Directive 2004 108 EC Certificate No 31361867 001C Holdar Photomotrics 3440 East Britannia Drive Tuscon Ad 85706 U S A Product Sclentific Digital Camera Identification M2del Number 3000Cclor inc 30 6 Monc Sericl Number 722106 1 Number 6co0Color inc s00CMono serial Number 034108 Tested acc to EN 55EC11 2COU ALl Part 1S Subpart 2 2C12 EN 61226 1 200G BN 61325 1 2013 BN 61C290 3 2 200 1442 EN 61090 3 3 2025 This certificate of conformity is based on an cvaluation of a sample of the product se specified in the certificate This is to cartify that the tested sarnple is in conformity with provisiona of Council Directive 2004 108 EC referred to as the EMC Directive This certificate does not Imply assessment of the production of the product and does not permit the use nf a TOV Rheinland mark of conformity Date 11 09 2013 5 Conan Boyte Laboratory Manager TUV Rheinland of Narth America Inc 1279 Quarry Lane Ste A Pleasantan CA 94565 Tcl 825 249 012 4 WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 Table of Contents INTRODUCTION U I III 1 System Components PrecautiIons 2 Environm
4. RETIGA 3000 IMAGING DIGITAL IMAGING MADE EASY www QIMAGING com x x A a 8 Xp Retiga 000 USER MANUAL Applicability This document applies to the Retiga 3000 camera For the latest updates please visit www qimaging com Notice of Copyright Copyright 2014 Quantitative Imaging Corporation All rights reserved Unauthorized duplication of this document is prohibited Trademarks and Proprietary Names QCapture Pro and Retiga are trademarks of Qlmaging PVCAM and Qlmaging are registered trademarks of Roper Scientific Inc Product names mentioned in this document may be trademarks or registered trademarks of Qlmaging or other hardware software or service providers and are used herein for identification purposes only Microsoft and Windows are registered trademarks in the U S and other countries of Microsoft Corporation and are used herein for identification purposes only Qlmaging Corporation Address Information 19535 56th Avenue Suite 101 Surrey BC Canada V3S 6K3 604 530 5800 WWW qimaging com Qlmaging Technical Support Technical support is available to all registered users of Qlmaging products from Yam to 5pm Pacific Standard Time support qimaging com 800 874 9789 http www qimaging com support contact WWW qimaging com 2014 Qlmaging All rights reserved Retiga3000 UM Rev A1 XE A d i Aay RS Em e BoP ERN N
5. Open before every exposure closed when not exposing Open Pre Sequence Open before start of sequence closed at end of sequence Open Pre Trigger Causes shutter to open before external trigger is received In non triggered mode operates as Open Pre Exposure Open No Change Sends no signals to open or close the shutter 19 ZH i as Sees z Retiga 3000 USER MANUAL Appendix A USB Information USB 2 0 Chipset Incompatibility Notice The USB 2 0 hardware used in the Retiga 3000 cameras is incompatible with new computers using native Intel USB 3 0 host controllers A third party PCle USB 3 0 card is required to resolve the problem Newer computers trom leading manufacturers including Dell and HP predominately use Intel based internal hardware and chipset controllers for managing the different components of the PC Nearly every computer now also includes the latest interface ports including USB 3 0 SuperSpeed However up until the Fall of 2013 while USB 3 0 interface ports were being introduced onto laptops and desktop computers the internal drivers and host controllers were actually from companies outside of Intel including Renesas and Texas Instruments With these computers Qlmaging USB 2 0 based cameras including the Retiga 3000 were tested and qualified to be fully functional However starting in the Fall of 2013 Intel released a new version of their mother board chips
6. Camera Dimensions Bottom 3 375 85 73 201 j 250 1 375 34 93 260 X 90 NEAR SIDE 2 465 62 61 07207006 COCO E gt O D FORORO 050 OCO OO O O gt 5 756 146 2 Additional Camera Measurements Dimensions 97 79 mm x 125 22 tn Ft v Camera weight 3 10 10 1 406 kg Optical interface 1 C mount optical format Mounting hole thread size 1 4 20 thread WWW qimaging com 92 15 Retiga 3000 USER MANUAL Connectors on Back of Camera Standard 20 Connector connector mates to Hirose Electrical Co Connector Part HR212 10P 10PC Power Switch Power Supply Specifications IMPORTANT Only operate the Retiga 3000 camera with the power supply provided by Qimaging Voltage output 5VDC 4A Voltage input 100 240 V 50 60 Hz positive polarity IMPORTANT Must not exceed 5V DC 4A Www qimaging com 2014 Qlmaging All rights reserved Retiga3000 Re 16 OT ise 2 SAT Retiga 3000 USER MANUAL Focal Plane Measurement SECTION T SCALES UT m is m PZZ TE n 1 ARE 85 Paris man vB 277 703 L LF DIA 7 Li TN lt A 204 5 18 COD IO OF WINDOW ADJUSTABLE C MOUNT RING 719 IMAGE SURFACE CCD 1MM FUSED GLASS SILICA WINDOW FOCAL PLANE 2
7. DAC and TTL signals Inputs must be at least 3 15 V for a high and less than 0 9 V for a low The numbers on the I O connector diagram correspond to the numbers given to the definition of each of the pins The I O trigger cable connects to Hirose connector HR212 10RC 10SDL 74 on the back of the camera Below is a description of each of the trigger pins PIN LOCATION For Hirose HR212 1ORC 10SDL Pin Signal Pin Signal Tigger Ready Out Shutter Output Ps Power Ready WWW qimaging com 2014 Qlmaging All rights reserved Retiga3000 UM Rev A1 18 c H ae vf 4 T CS set Retiga 3000 USER MANUAL Triggering signal behavior Trigger In the input trigger to the camera Camera Expose represents when the camera is exposing acquiring an image Read Out represents the readout of the image Shutter Output TTL output to control timing of an external shutter driver Trigger Ready represents when the camera is ready to receive a trigger Power Ready reflects power status for the camera 5 V on 0 V off Ground system digital ground Types of triggering supported by the Retiga 3000 Trigger first mode Overlap Non Overlap Strobe mode Overlap Non Overlap Bulb Mode Non Overlap Five shutter behavior modes are available www qimaging com 2014 Qimaging All rights reserved Retiga3000 UM Rev A1 Open Never Shutter is always closed Open Pre Exposure
8. Roct Hub 4 Generic USB Hub b SafeNet Inc USB Key SafeNet USB SuperPro UltraPro Intel R 82801 PCI Bridge 244E gt e Intel R HD Graphics 4000 Figure 2 Photometrics USB2 camera is the only device on its USB Root Hub WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 21 a Retiga 3000 USER MANUAL If more devices are connected to the same USB Root Hub please reconnect them to a different port for optimal camera performance or use the supplied USB 3 0 card For any further questions and assistance please contact Qlmaging technical support WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 22 FUIMAGING DIGITAL IMAGING MADE EASY www qgimaging com
9. camera system be sure to turn the camera power on last and power the camera off first m Never connect or disconnect any cable while the camera system is powered on m Although you should turn off the camera s power supply before disconnecting any camera system cable you do not need to power off your computer to detach the cables m Use caution when triggering high current switching devices such as an arc lamp near your system The CCD can be permanently damaged by transient voltage spikes If electrically noisy devices are present an isolated conditioned power line or dedicated isolation transformer is highly recommended m Always leave at least one inch of space around the camera housing Never open the camera There are no user serviceable parts inside the Retiga 3000 camera Opening the camera voids the warranty m Use only the integrated USB 2 0 interface cables and power supply designated for this camera system Using non Retiga 3000 cables or power supplies may result in permanent damage to your system m Do not use a C mount lens that has optics that extend behind the flange of the lens Environmental Conditions for Operation and Storage Your Retiga 3000 camera system should be operated in a clean dry environment The camera system s ambient operating temperature is O C to 30 C with 80 relative humidity noncondensing Store the Retiga 3000 camera system in its original containers To protect the system from exces
10. is set to the On position m Confirm that the correct Retiga 3000 camera is selected in your imaging software application m Power off the camera and the host computer and check all system connections Restart m Confirm that the camera is operational by taking an image with a standard C mount lens attached to your Retiga 3000 Using normal room lighting place the camera on a table about 3 meters away from an object and acquire an image If the camera is powered on and recognized by the computer and still is not able to capture an images please contact 8 Support PVCAM Error Message Appears If a PVCAM error message appears please note the message s number code and contact Qlmaging Support Lengthy Pauses During Imaging If you notice lengthy pauses marked by a lot of disk activity while imaging m Close any other programs that may be running m nstall more physical memory to your computer system 13 x ee 2 Retiga 3000 USER MANUAL Camera Fan Not Running Check the set point temperature in your software application Make sure it is set to a temperature cooler than 5 The stabilizing temperature when the fan is turned off is 5 C Try modifying the sensor temperature to see if the fan turns on Contact Qlmaging Support if the fan still does not run Camera Running Too Warm It is normal for the camera to be slightly warm to the touch while in operation However if the camera is more th
11. the camera s sensor Gain plays a critical role in many of the camera s parameters including dynamic range and read noise The Retiga 3000 provides three user selectable gain states enable optimal camera performance for different imaging environments m Gain State 1 1 8e ADU 0 5x Gain High Light 1 2 single pixel full well max bit depth m Gain State 2 1 2e ADU 1x Gain Mapped single pixel full well max bit depth m Gain State 3 0 3e ADU Gain Low Light 1 3 single pixel full well max bit depth WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 H ST is lt Retiga 3000 USER MANUAL Gain State 2 uses the full dynamic range of the camera and is recommended for bright field applications Gain State 3 although only using a third of the single pixel full well is ideal for resolving low luminescence signals Therefore Gain State 3 is recommended for fluorescence applications Sensor Cooling To reduce thermally generated noise attributable to dark current H the Retiga 3000 camera system provides stabilized sensor cooling of O C at up to a 30 C ambient room temperature Additional Parallel Clockirg temperature control is available to allow flexibility in reaching E different stabilized sensor temperatures for various ambient room a conditions
12. 1x1 for capture Adjust exposure time for binning applied Set for preview mode only Captured image should be full frame binned 1x1 All other settings should be locked Full frame no ROI Normal mode Clear Pre Exposure Gamma 1 30ms exposure Retiga 3000 USER MANUAL Retiga 3000 Color Camera Default settings 24 bits Gain State 2 Binned 1x1 Center quad ROI for live preview Set for preview mode only Captured image should be Tull frame All other settings should be locked Www qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 Normal mode Clear Pre Exposure Gamma 1 10ms exposure RGB values BgeS TS 12 WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 Retiga 3000 USER MANUAL CHAPTER 4 TROUBLESHOOTING Resolving Problems with the Camera Computer Does Not Recognize Newly Installed Camera Confirm that the latest PVCAM camera driver is installed on the computer while also checking to make sure the computer meets the minimum system configuration requirements to run the Retiga 3000 Confirm that the camera s power switch is set to the On position Then power off the camera and the host computer and check all system connections Restart If a New Hardware Found dialog box still does not appear contact Qlmaging Support Images Not Displayed If no images appear m Confirm that the camera s power switch
13. MM 1MM IR FILTER FOR 3000C amp 6000C ONLY Sensor Specifications Image type Color or Monochrome Scientific CCD depending on camera model Dimensions 8 8mm x 6 6mm 11mm diagonal Resolution 1940 x 1460 2 8 MP Pixel size 4 54 um x 4 54 um Single pixel full well 16 000 e 24 000e binned 2x2 Peak QE 75 600 nm measured using monochrome model Readout frequency 20 MHz Cooling 0 C stabilized Www qimaging com 2014 Qimaging All rights reserved QI_Retiga3000_UM_Re i E E EJ ESSN Retiga 3000 USER MANUAL Sensor Orientation 2750 0 0 REFERENCE 0 0 y 1460 When the Retiga 3000 is coupled to the side port of an inverted microscope with the flat surface of the camera parallel to the table the CCD orientation will match the view seen through the eyepiece This allows for easy access to the mounting holes to stabilize the camera while maintaining a consistent perspective between the captured images and what s seen down the eyepiece of the microscope For the top port of upright microscopes and bottom ports to some inverted microscopes the Retiga 3000 must be rotated 90 counter clockwise to match the view seen through the eyepiece This is due to the differences in the optical paths between inverted side ports and upright top ports I O Connector Pinout The I O Input Output Status connector provides information about trigger function
14. The sensor temperature is set via software Shutter Open Delay Shutter Command Clear Cycles S Additionally the Retiga 3000 has the option of disabling its internal TI Horizontal fan for vibration tree and or electrically sensitive applications The Flip Vertica camera cp camera s fan can be turned off by setting the temperature to 5 C Clocking Mode The Retiga 3000 camera has two CCD clocking modes one of which allows removal of the glow that can appear in an image s corners at longer exposures arising from preamplifiers In the PVCAM implementation the clocking modes are referred to as Normal and Alternate Normal In Normal mode the CCD is optimized for precise exposure time control and maximum frame rate n Alternate Normal mode the preamplifier is switched off during the exposure to eliminate the background generated by preamplitier glow However switching the preamplifier off and on again adds additional time to each exposure especially noticeable when in Overlap mode defined below Since the actual exposure time is slightly longer than the entered exposure time Alternate Normal mode will increase the image brightness due to the increase in light collected by the slightly longer exposure as well as slightly decreasing frame rate As a result it is usually beneficial to operate the camera in Alternate Normal mode for long exposures gt 1 sec where preamplifier glow begins to
15. an slightly warm to the touch and at least one inch of space has been left around the camera housing switch off the camera immediately and contact Qlmaging Support Unresolved Problems Contacting Qimaging Support If you are still unable to resolve your problem contact Qlmaging Support for assistance in one of four ways m Visit http www qimaging com support faq for a list of all frequently asked questions Your issue may be resolved in one of these faqs m Visit http www gimaging com contact and fill out a support form online m E mail support qimaging com with complete details of your problem including Error Message and Code if possible camera model computer hardware configuration and operating system m Call one of Qlmagings regional support offices at the appropriate number below To assist you better and to quickly resolve the issue it is recommended that you are at your computer during the call United States and Canada Qlmaging Tel 1 800 874 9789 China Shanghai Roper Industries Trading Co Ltd Tel 86 21 3377 3519 Japan Nippon Roper K K Tel 81 3 5639 2731 Europe Roper Engineering s r o Tel 420 597 305 857 www qimaging com 2014 Qimaging All rights reserved Retiga3000 UM Rev A1 14 f 3 EN H ASAE x CEN t SRA SSS VANS Retiga 3000 USER MANUAL CHAPTER SPECIFICATIONS Camera Dimensions Front 4 930 125 22 i 3 850 177 771
16. arty imaging software applications please visit http www qimaging com resources pdfs Software Compatibility Matrix pdf The Retiga 3000 camera s image capture capabilities are controlled entirely through your imaging software Basic functionalities include control over exposure time gain state clearing mode region of interest ROI and pixel binning For information describing how to implement changes to the camera s parameters refer to your imaging software s instruction manual Basic Camera Parameters Exposure Time The Retiga 3000 camera s exposure controls allow you to adjust the integration time for each acquisition 0 ms 30 min By increasing the exposure time more light is captured by the sensor and a better signal to noise ratio SNR is achieved The longer the exposure time however the longer you also expose your cells to more light increasing the effects of photo toxicity and photo bleaching The exposure time should be adjusted to a level that achieves the shortest integration time possible while still maintaining sufficient SNR Gain State Gain with regards to cameras is defined as the conversion factor of captured electrons to a digital signal often referred to as a grey value or ADU Analogue to Digital Unit and has units of electrons per ADU e ADU Knowing the gain of a camera allows users to directly compare an ADU value as measured from their software to the physical number of electrons actually captured by
17. be noticeable and in Normal mode for short exposures 1 sec where frame rate and precise exposure time are more critical ur TN WLS a WSA 9 SES 2 T p E Se y 2 K D i gt ag B 4 P P a N ME ae 2 Hm Retiga 3000 USER MANUAL Clearing Mode When the camera does not expose and read out images simultaneously it is in Non Overlap mode Non Overlap mode is set by choosing Pre Exposure Clearing for the clearing mode of the camera This allows a clear before each exposure The following waveforms show how Non Overlap mode functions The main benefit of Non Overlap mode is that there are no limitations imposed upon the exposure time and the set exposure time is the actual exposure time The tradeoff for this accuracy is the frame rate as each frame must be completely read out before beginning the next exposure Clears Exposure Read out 1 Read out 2 Read out 3 Readout When the camera is able to expose and read out images simultaneously it is in Overlap mode Overlap mode is set by choosing Pre Sequence Clearing for the clearing mode of the camera This allows one clear before the imaging sequence starts The following waveforms show how Overlap mode functions When using Overlap mode the frame rate is higher as compared to Non Overlap mode and provides the ability to continuously image However since expo
18. camera voids the warranty a WWW qimaging com 2014 Qimagino All rights reserved Retiga3000 UM Rev 1 gt Vas Soi Se SEO Retiga 3000 USER MANUAL CHAPTER 2 INSTALLATION This chapter will detail the proper installation of your Retiga 3000 camera In order to install and use the camera the following items are required Retiga 3000 camera supplied USB 2 0 data cable supplied 5V 4A power supply Supplied USB installer flash drive Supplied StarTech 2 Port PCle SuperSpeed USB 3 0 Card Adapter supplied 110V 220V power connection A PC that meets host computer requirements Host Computer Requirements The host computer for your Retiga 3000 camera must include Windows 7 operating system 32 64 bits 2 0 GHz or faster Intel processor either Xeon or Core 15 4 GB RAM 250 GB serial ATA SATA HDD and or gt 64 GB solid state drive SDD for high speed imaging and storage 256 MB slot based ATI NVIDIA video graphics card i e not an onboard integrated graphics adapter CD ROM drive or internet access to install the driver One available PCle slot NOTE Minimum requirements as of January 2014 Supported computer systems will change as new cameras computer hardware and operating systems are introduced and older models then become obsolete For current information on recommended computer specifications please visit http www qimaging com support recommended compu
19. cting the camera to the computer use the supplied USB flash drive to run the PVCAM installer executable Be sure to select the appropriate installer based on your PC s operating system 32 bit or 64 bit After completing the PVCAM installation restart your PC when prompted by the wizard Next install the QCapture Pro imaging software from the USB flash drive The camera is now ready to connect to the computer Make sure the camera s power switch is set to the Off position NOTE Supplemental PVCAM installation information can be found on the Qlmaging website http www qimaging com support downloads Www qimaging com 62014 Qlmaging All rights reserved Retiga3000 UM Rev A1 7 TNAM 2 2 muon M D Retiga 3000 USER MANUAL Installation Step 3 Connect the Camera In addition to a power switch there are three connectors on the back of the Retiga 3000 camera a USB 2 0 connector a power supply connector and an I O connector Connect the camera following these steps IMPORTANT Ensure the power switch is set to the OFF position and before connecting the camera 1 Connect the supplied USB cable to the interface port on the USB 3 0 PCle card now installed on your computer and connect the other end of the cable to the USB 2 0 connector located on the back of the camera The USB interface transfers camera settings and image data to the host PC Please see Appendix A for more detailed information on connecting t
20. ental Conditions for Operation and 5 2 Microscopes Lenses Tripods 2 Cleaning nentes 3 Repalr a I III 3 INSTALLATION IIS 4 Host Computer Requirements e NT TN TO s 4 Camera Power Requirements l TRENT T NN RR 5 Installation Step 1 Install the USB 3 0 PCle 5 Installation Step 2 Install the Camera Driver TT 5 Installation Step 3 Connect the Camera 6 USING YOUR RETIGA 3000 CAMERA K sn 7 Imaging Software ee elspa T CC DER 7 Basic Camera Parameters mn 7 Exposure Time 7 Gain State tt 7 Sensor Coolind 8 Clocking Mode I III 8 Clearing 9 ROI ERUNT INI s 10 Device Synchronization TRENT DERE 10 TROUBLESHOOTING
21. ets Series 7 C216 Series 8 C220 and later with a native Intel based USB 3 0 host controller USB3 extensible host controller xHCI in the Device Manager On these newer PCs the Intel USB 3 0 host controller does not communicate properly with some traditional USB 2 0 chipsets to the extent where data is not properly delivered This hardware incompatibility is unfortunately the cause of recently discovered bugs with the Retiga 3000 cameras and new Dell and HP computers These problems manifest as black frames or failed frame deliveries when attempting to view live or capture images and is only seen on PCs that have USB 3 0 ports and native Intel host controllers If a PC or laptop has only USB 2 0 ports or USB 3 0 ports that use third party host controllers the cameras will be fully functional However most new computers purchased in 2014 will likely have Intel based host controllers To alleviate this problem each Retiga 3000 camera now includes a StarTech 2 Port PCI Express SuperSpeed USB 3 0 Card Adapter This card installs an independent Renesas host controller which allows the Retiga cameras to run properly even on newer desktop computers As this workaround requires a separate USB 3 0 card this solution is only viable for desktop computers and not laptops For the immediate future there is no solution for laptop computers which are therefore are not supported by these cameras Qlmaging is currently working with the engineering team at Intel
22. he Retiga 3000 to a native USB port on the computer 2 After the USB 2 0 cable has been connected to the computer and camera connect the Retiga 3000 power supply s cable to the power connector located on the back of the camera Your Retiga 3000 camera is powered by the 5V 4A power supply provided by Qlmaging Lastly plug the power supply5 cord into an appropriate power source You may now power on your camera by setting its power switch to the On position 4 Optional An I O connector is available on your camera for optional hardware triggering The I O connector provides multiple I O signals that allow highly precise synchronization with external hardware components such as light sources and motorized microscope stages via TTL signals Information on how to set up and configure the Retiga 3000 camera for hardware triggering can be found in the next chapter of this manual Power 5V 4A Standard USB 2 0 Connector connector mates to Hirose Electrical Co Connector Part HR212 10P 10PC Power Switch WWW qimaging com 2014 Qlmaging All rights reserved Retiga3000 UM Rev 1 c Vas W See SEO Retiga 3000 USER MANUAL CHAPTER 5 USING YOUR RETIGA 3000 CAMERA Imaging Software The easy to use QCapture Pro software supplied with your Retiga 3000 provides complete control over the camera s settings and image capture functions For an up to date list of other compatible third p
23. illion pixels a 4 54um pixel pitch and a high quantum efficiency of 75 this camera provides higher resolution and sensitivity than most standard fluorescence microscopy cameras Available monochrome LI color the Retiga 3000 uses a USB 2 0 data connection for a fast and easy installation Additionally low noise electronics cooled sensor technology and excellent quantum efficiency QE yield superb sensitivity for low light fluorescence applications RETIGA This manual describes the installation and configuration procedures for your new Retiga 3000 color or monochrome camera Descriptions of Retiga 3000 camera settings are also included System Components Your new camera system includes m Retiga 3000 camera m 5V 4A power supply m 1 Meter USB 2 0 cable m Black lens cap m USB flash drive PVCAM driver QCapture Pro installer manual datasheet training materials m USB security key required for QCapture Pro software m StarTech 2 Port PCI Express PCle SuperSpeed USB 3 0 Card Adapter WWW qimaging com 2014 Qlmaging All rights reserved Retiga3000 UM Re H 2 vf 4 22 x Retiga 3000 USER MANUAL Precautions The CCD and other Retiga 3000 camera system electronics are extremely sensitive to electrostatic discharge ESD To avoid permanently damaging the system please observe the following precautions m f you are using high voltage equipment such as an arc lamp with your
24. out 1 Read out 2 Read out 3 Www qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 10 g Ex D SI gt fan A A gt V v A a y OW e Retiga 000 USER MANUAL Strobe mode In this mode each frame in the sequence requires a trigger When a trigger is received the camera exposes for the time set in the software If Strobe mode is set to run in Overlap mode then all exposures except the initial one will be equal to or larger than the readout time Triggers received while Trigger Ready is low are ignored For a sequence of one frame Strobe mode and Trigger first mode are the same Non Overlap mode is shown on the left Overlap mode is shown on the right Trigger Trigger Trigger Trigger Ready Ready Exp 1 Exp 2 Exp 3 Exposure Exposure Readout Readout Bulb mode In this mode each frame in the sequence requires a trigger The camera exposes for the duration the trigger signal is high Exposure time entered into the software is not used in this mode Triggers received when Trigger Ready is low are ignored Non Overlap mode is shown below Trigger Ignored Trigger Ready Exposure Readout WWW qimaging com 2014 Qlmaging All rights reserved Retiga3000 UM Rev 1 11 Recommended Settings Retiga 3000 Monochrome Camera Default settings 14 bits Gain State 2 Binned 2x2 for live preview binned
25. quired images for documentation and publication purposes When using pixel binning the acquired images will appear to be grayscale although they will have an RGB pixel value Simply convert the images to a single channel greyscale value 8 bit mono 12 bit mono etc and use your software application to apply a color tint Device Synchronization Your Retiga 3000 camera offers several methods of hardware synchronization via TTL signals with external devices including function generators light sources shutters and filters Each camera has an I O connector pin out functions are described in the specifications chapter on the back for trigger in out and various TTL input and output operations A special cable is available to access primary signals such as Trigger in Expose out and Frame Readout In the default mode the camera triggers on the rising edge of a TTL signal Retiga 3000 cameras support three trigger modes Trigger first mode In this mode the camera requires one trigger to begin the acquisition of a stream of images Once a single trigger is received the camera will use its internal clock to acquire the entire image stream independent of any future triggers It is possible to run this triggering mode in either Non Overlap mode left or Overlap mode right Trigger Trigger Trigger Trigger Read Read Exp 1 Exp 2 xp 3 Exp 1 Exp 2 Exp 3 Exposure Exposure Read out 1 Read out 2 Read out 3 Readout Read
26. sive heat cold and moisture store at an ambient temperature between 20 C and 60 C with a relative humidity of 096 to 9096 noncondensing Microscopes Lenses Tripods Retiga 3000 has a standard threaded video mount and can be mounted to any microscope that accepts a standard C mount adapter The camera also allows you to install any lens that is compatible with a standard threaded video mount as long as its optics do not extend behind the flange of the lens The Retiga 3000 camera can be mounted to a tripod using the tripod mounting attachment located on the bottom of the camera See the Specifications chapter for more information WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 f _ wa Retiga 3000 USER MANUAL Cleaning Clean the exterior surfaces of the camera with a dry lint free cloth To remove stains contact Qlmaging Support To clean the camera s imaging window use only a filtered compressed air source Handheld cans are not recommended as they may spray propellant onto the window Do not touch the window Repair The Retiga 3000 camera system contains no user serviceable parts Repairs must be done by Qlmaging Should your camera system need repair contact Qlmaging Support Please save the original packing materials so you can safely ship the camera system to another location or return it for repairs if necessary IMPORTANT DO NOT OPEN the camera Opening the Retiga 3000
27. sure and readout occur simultaneously the minimum exposure time is dependent on the time taken to complete readout As such excluding the first frame any exposure time smaller than the readout time defaults to an exposure duration equal to the readout time Clears Exp 1 Exp 2 Exp 3 Exposure Read out 1 Read out 2 Read out 3 Readout www qimaging com 2014 Qimaging All rights reserved Retiga3000 UM Rev A1 gc lt EI m WSS 22 C iie B SSS _ 52 4 zr s Y E Retiga 3000 USER MANUAL ROI Binning The Retiga 3000 supports both user defined arbitrary regions of interest ROI as well as hardware pixel binning modes of 1x1 2x2 4x4 and 8x8 Both ROIs and pixel binning will enable faster frame rates making focusing and scanning much easier Pixel binning also has the advantage of significantly increasing the signal to noise ratio SNR of the image For color cameras pixel binning will result in the loss of the native color interpolation When using the Retiga 3000 color camera for low light fluorescence documentation binning can be used to improve SNR but pseudo coloring will be required to obtain color images Pseudo coloring is a commonly used method and is a function typically available in most imaging software applications including QCapture Pro 7 This will allow you to apply the tint of the fluorescent labels used to the ac
28. ter specifications php WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 Retiga 3000 USER MANUAL Camera Power Requirements m 5V DC maximum A power supply for use with your Retiga 3000 camera has been provided by Qlmaging IMPORTANT Follow these steps in order DO NOT CONNECT the camera until the driver is installed Installation Step 1 Install the USB 3 0 PCle Card In order to guarantee optimal camera performance it is recommended that you use the supplied USB 3 0 PCle card over any native USB ports on the computer This will provide the necessary bandwidth and avoid any memory allocation competition from other devices To install the USB PCle card please follow these steps m Shut down your computer m Open the case and install the included USB 3 0 card into an empty PCle slot See the user s manual for your computer for complete instructions on installing new PCI Express cards m Restart your computer m Use the CD supplied with the USB card to install the appropriate drivers Refer to the USB card package for further details on installation If you elect to use a native USB port on your computer instead of the USB card please refer to Appendix A for more information on USB port compatibility Installation Step 2 Install the Camera Driver In order for the Retiga 3000 to communicate with the host PC the camera s device driver PVCAM must first be installed Before conne
29. to further investigate this issue and will continue to work towards a long term solution that will not require a third party USB card For any further questions and assistance please contact Qlmaging technical support WWW qimaging com 2014 Qlmaging All rights reserved QI Retiga3000 UM Rev A1 20 22 i Retiga 3000 USER MANUAL Determining if a USB port is on a Root Hub As discussed above if a PC or laptop has only USB 2 0 ports or USB 3 0 ports that use third party host controllers the cameras will be fully functional and the supplied USB 3 0 card is not required If you elect to use a native USB port rather than the supplied USB 3 0 card please ensure that the port you use is on its own dedicated Root Hub with no other devices connected Each USB host controller on a PC or laptop has a Root Hub to which multiple physical ports are connected Each port can have a device plugged in or another hub to allow connection of more devices However the Retiga 3000 camera should be the only device connected to a single USB Root Hub If other devices are connected to the same Root Hub then camera communication may fail which can result in camera hanging or image tearing Since it is not always straightforward to tell which port on a PC Is connected to which Root Hub follow the procedure below to make sure that the camera is the only device connected to its particular Root Hub 1 Open Device Manager click the Start B
30. utton and type Device Manager 2 Inthe menu click View gt Devices by Connection Figure 1 3 According to Figure 2 find the USB host controllers expand them and check whether your camera is the only device connected to the Root Hub File Action View H rnis ACP 564 based PC Microsoft ACPI Comphant System ACPI Fan ACPI Fan ACPI Fan ACPI Fan ACPI Fan ACPI Fixed Feature Button ACPI Thermal Zone ACPI Thermal Zone Intel R Core TM 5 3570 CPU 3 40GHz Intel R Core TM iS 3570K CPU 3 40GHz Intel R Core TM 6 3570K CPU 3 40GHz Figure 1 Devices by connection Intel R Core TM i5 3570K CPU 340GH ao PCi bus 4 VK High Definition Audio Controller High Definition Audio Device amp VIA High Definition Audio 98 Intel R 7 Series C216 Chipset Family PCI Express Root Port 1 1610 98 Intel R 7 Series C216 Chipset Family PCI Express Root Port 5 1 18 98 Intel R 7 Series C216 Chipset Family PCI Express Root Port 7 1HC W Intel R 7 Series C216 Chipset Family PCI Express Root Port 8 161E 7 Series C216 Family SATA AHCI Controller Devices by connection Resources by type Resources by connection Show hidden devices Customize 98 ACPI Fan 388 ACPI Fixed Feature Button ACPI Thermal Zone PD 7 Series C216 Chipset family USB Enhanced Host Controller 120 4 M USB Root Hub 4 Generic USB Hub USB
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