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Bio-Plex Pro™ & Bio-Plex Pro II Wash Stations

1. 53 INTRODUCTION MN RR DU REN NUT 53 BEROGRAMTMENE S a a eee ar EIE DM 53 DERNE EDITA PROGRA TTE TU UTER 54 rm 55 PIOCESS DISDBISD dU ipe D MI ace aw Bae 56 PIOCCSS VV MCI II 58 PIOCCSS SIC DEDIMUS 59 PYOCESS SIE USer PIOIMID cm 59 Process Slop VACUO FINI BON Pm 59 SHOW PROGRAM A E EE 60 CLEAR FE ROGRA Where EER m 61 SEPPINGS MENU RR n 61 TETUR 62 QOS SUING RU Pm 62 VOCE diis i RR t 62 BUOD SONS O ECCE TREE TE 63 PROCEDURES MEN E Ac ia eu E M M PM A M MED EN 63 RI EDT 63 STREET 63 ETODIVA HBIO cod cip n tp depot urbis fat dabat 63 MAINTENANCE AND CLEANING 65 CUEANING PROCEDURES MEM teu D E Aic t E MI ELLA 65 Vi Bio Plex Pro and Bio Plex Pro II Wash Stations Cleani
2. 40 Precautions When using vacuum plate carrier 24 Prime Tray 63 Priming mij 21 PIOW Rio PETER 21 VATI DOLO 20 pw 25 Leaving instrument 26 Leaving instrument on sese 26 RINSED Iy Pero ee ees 25 RINSE NIODEE s e airo teneat 26 39 cel c ee eee ee eer ete ere eee eee 67 Tubing BIacKel ii iret ee edie 16 UNPACKING EN DRE 40 Vacuum Filtration DIS ADC cT T 18 ona a a 18 Wash Programs QUICK GUII 23 PRUNING erem 22 I II 23 Working Area Requirements 40 Bio Plex Pro and Bio Plex Pro Wash Stations Bio Plex Pro and Bio Plex Pro II Wash Stations 81 Life Science Group 10013125 US EG Bio Rad Laboratories Inc Web site www bio rad com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 8901 Belgium 09 385 55 17 Brazil 55 21 3237 9400 Canada 905 364 3435 China 86 21 6426 0808 Czech Republic 420 241 430 532 Denmark 44 52 1000 Finland 09 804 22 00 France 01 47 95 69 65 Germany 089 318 84 0 Greece 30 210 777 4396 Hong 852 2789 3300 Hungary 36 1 455 8800 India 91 124 4029300 Israel 03 963 6050 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The
3. Movement of manifold 57 Defining New Programs Process Step Wash This process step is used to aspirate solution out of the wells and simultaneously dispense and aspirate the solution CX PX WASH EXIT OK CROSSW ASP NO EXIT OK POS OVERFLOW EXIT OK VOLUME 300yl EXIT OK CHANNEL 1 EXIT OK H SPEED 10 mm s EXIT OK FL RATE 200 ul s EXIT OK ASP RATE 2 EXIT OK 15 CX PY WASH EXIT OK 58 Select and confirm wash step WASH CX Cycle Number PX Program Step Number Select Yes using or to perform aspiration of wash crosswise or select No to perform normal aspiration Select required dispensing position OVERFLOW CUSTOM MOVE OVER MOVE CUST or BOTTOM Select the volume of solution to be used 50 3000 ul in increments of 50 ul Select the channel to be used 1 4 depending on instrument configuration Select required aspirating speed 1 20 mm sec Select required flow rate drip to 500ul sec Select required aspirating rate 1 3 Select required aspirating time 1 20 mm s After the process step has been defined the initial display appears CX Cycle Number PY Program Step Number incremented by 1 Press OK to define another wash step or press or to select the next program step Program End or a new cycle Bio Plex Pro and Bio Plex Pro Wash Statio
4. a cathe DM AIL EE 40 POWER REQUIREMENTS ree cg cece ase eee LL aM M M M RE MR EM M E 40 UNPACKING AND INSPECTION CHECKLISTS iis dirt dud towed exque cav 40 DETAILED OPERATING INSTRUCTIONS Y cua Ceo YexwaE WES E Eo NEM XU 42 SWITCHING THE INSTRUMENT as uie d be LE emus Dato M Dp Ce Grade dara sc dea ierit 42 COMPLETE INSTRUMENT KEYPAD PUNCTIONS oeseonesuutsudtutustadz kB erase howext erue But ees entoc etes t det edes 43 Alphabetical list of LCD text With explanations eiiis essen nhanh hannis naar nnns 45 DEFINING GUSTOM WASH PROGRAMS ss vts va MR ox VCem US oI Ea 48 NG IO DS E ERR A 48 FOSON M 49 INS PIRATING sccnouzvuctesactavnaduaceutcasuateuamnnaubtcoastanss dauivardacnstvsneuncuatcoatsastoncounsdutaauaabietvacatansatussuatcoss ct 50 Normal ERE E 50 E otc uIPiToesumusc 50 DEP NOE the rt ne WO NM EM 51 DEFINING NEW
5. 29 AS 0 HERMANNO Tr 29 Yearly Service Engineer Hequired eee E nean rank ansa a I MR ARS a a IM RN AA NER Ya saa Ra naaa 29 Bio Plex Pro and Bio Plex Pro II Wash Stations V SECTION pe 30 PURPOSE OF REFERENCE SECTION eden tte etm rtu dp e AAT E Mt AP M AM E Med MU 30 INSTRUMENT DETAILS eor a a Cibo s Ea X n ES dq ot me dra LO RE e x a rem RN ER Ew Kw au e o va 31 INSTRUMENT GAFE TY arau oe ath ete teme Hor LLLA er c OA LE ML A E 31 INTENDED USE 21 teios cmt ite edet het erat oM ad nal Eta op M 32 SONIC RR TIONS ola eee tate here uA EE ML i RANA cel 33 INSTRUMENT SPECIFICATIONS Sac ur euet us e Stereo teu Ceu be esce 34 DESCRIPAIONIRa rd uet geste EIL MM M MA MA RN UM El DAN rei ie 36 BIOFLEX PRO DIMENSIONS 37 BIOSPEEX PROI DIMENSION iot 38 REAR PANEL CONNECTIONS rU cda atone Lee LAN Stud Pr Mu DOR rU A Retard 38 mis ead dz BE ROREM 39 DETAILED INSTALLATION sx RE a aa a Rau EE d du RON Ya TR DO NR E NENNT 40 BEQUIREDANORKINGUABEAR nS dt cssc apta nxorem ME Du Mec
6. Turning the Power The power switch is located at the rear of the instrument above the power cable connection Make sure the instrument is plugged in using the provided power cable before you attempt to operate Bio Plex Pro and Bio Plex Pro 11 Wash Stations 19 Basic Operation and Maintenance Keypad Basics All the functions you need to run Bio Plex programs are easily accessed using the keypad on your wash station The system is organized into primary and secondary menus Primary Menu The instrument menu has the following options Primary Secondary Menu Options Menu Options Programs Wash programs used to process assays Start Define Edit Show Clear Settings Rarely accessed advanced features Edit Plates Options Bubble Sensor Procedures Preparation or maintenance processes Prime Rinse Empty Prime Tray Vacuum Filtration Secondary Menu If you choose OK when any of the primary menu options Programs Settings and Procedures are visible on the LED you will navigate to the secondary menus You may Select a choice from the secondary menu or press Exit to return to the primary menu If you get confused about LCD PROGRAM where you are press Exit lt gt until you no longer see Exit as an option buttons Navigate Select or to other Exit options Priming the Instrument You should prime both Channel 1 and 2 after installation and if the instrument has not been used for an extend
7. 76 PC PIOCESS Conto System ENTON orriari AEEA EEEE 77 INO Aspiration at VACUUM Filralon EN Ol s carat ii parri ua acr d e E HE C DR c Cn HD TF EADE 5118 E A eer eee er eee ee ene eee 78 DECLARATION OF CONFORMITY 2 2 uaa DRE E i 79 INDE aici nit ln nd aia waite 80 Indicates the possible presence of biologically hazardous material Indicates the possible presence of a strong magnetic field Warnings Cautions and Notes The following types of notices are used in this publication to highlight important information or to warn the user of a potentially dangerous situation Information Gives helpful information Bio Plex Pro and Bio Plex Pro 11 Wash Stations vil Caution Indicates a possibility of instrument damage data loss if instructions are not followed WARNING Indicates the possibility of severe personal injury loss of life or equipment damage if the instructions are not followed WARNING This symbol Indicates the possible presence of biologically hazardous material Proper laboratory safety precautions must be observed Attention Negative environmental impacts associated with the treatment of waste e Do not treat electrical and electronic equipment as unsorted municipal waste e Collect waste electrical and electronic equipment separately WARNING Risk of fire and explosion Ethanol is flammable and when improperly handled can l
8. udi 10 38 SN 63 Bubble 2 22 e reta secta 63 Checklists UID ACK ING ne 40 Cleaning Cover and Display 65 tanta tos agis t Dau tbe itas ins 65 ee 66 Waste cedo P Un 66 Configuration ANG plate uice et a cur eto 33 EE R 11 Custom Wash 48 53 Diagram AMC NE 39 Installation of Bio Plex 14 Installation of Bio Plex Pro 11 15 adio 20 SLOT erpa eE 36 Dimensions joris RETE 37 plospiex PrO sitcom eui dor uetus tutus 38 DISIMOC IOR E Eo 71 POC OMIT 72 SOIBHDLIS 71 Keypad 20 28 and the 20 NAVIGATING eR 20 enm 62 QU m 20 PAMA Aere 20 D PLE 63 udis rz lange cL n 53 QUICK Gde iiu Uber eun ae 24 80 wlan 20 Singer 61 Microplates Iristallatioli scole n e eroe eot boe Cento m buds 19 12 19 42 Plate 17 ES 17 Removal 17 SEIEUN 17 Power
9. CYCLE The number of cycles the subsequent processing steps should be performed An aspirate step removes liquid from the wells DISP A dispense step fills the wells with liquid Liquid is dispensed and aspirated simultaneously creating a circular flow with a maximum volume of 3000 ul in wash step for increased wash efficiency During a soak step the liquid remains in the wells for the set time with or without shaking For collecting desired substances by vacuum filtration using filtration plates USER PROMPT User interaction is requested FINAL ASPIRATE An aspirate step at the end of a program END PROGRAM The program finishes Program Menu The Program menu has the following options pianon NN The Program menu has the following structure PROGRAM lt gt OK START DEFINE EDIT SHOW lt gt EXIT OK EXIT OK E gt EXIT OK Bio Plex Pro and Bio Plex Pro Wash Stations 53 EXIT OK Defining New Programs Define Edit a Program DEFINE EDIT lt gt EXIT OK PROG 1 NAME EXAMPLE 567890 ABCDE PLATE MODE EXIT OK Plate 1 GRE96ft EXIT OK NO OF CYCLES 1 4 EXIT OK C1 P1 ASP EXIT OK C1 P2 ASP EXIT OK C2 CYCLE EXIT OK PROGRAM END FINAL ASP NO YES EXIT OK 54 Press to define edit a program the instrument Press or to select a
10. EXIT OK PLATE 1 GRE96ft NO OF CYCLES 2 EXIT OK C1P1 WASH EXIT OK POS MOVE CUST EXIT OK Z User Def 7000 VOLUME 450 EXIT CHANNEL 1 EXIT H SPEED 1mm s EXIT OK FLOWRATE DRIP EXIT OK PROGRAM END EXIT OK FINAL ASP NO YES To define a program press OK to enter the DEFINE EDIT menu Select an appropriate position for the program Select an appropriate name for the program Select PLATE MODE and press OK Select an appropriate plate type and press OK Select 2 cycles and press OK Select WASH and press OK Select MOVE CUST and press OK to continue to the next parameter Set the user defined Z position to the appropriate height and press OK to continue to the next parameter Set the volume to 450 ul and press OK to continue to the next parameter set the channel to 1 and press OK to continue to the next parameter Set the head speed to 1 mm s and press OK to continue to the next parameter Set the flow rate to DRIP and press OK to continue to the next parameter Select PROGRAM END and press OK When the final aspiration step appears press NO to finish defining the program Example of a typical wash program for working with adherent cells 52 Bio Plex Pro and Bio Plex Pro II Wash Stations Defining New Programs Defining New Programs Introduction The following program items are available
11. T Enable Low VAC EXIT OK pue lt gt Clean your vacuum plate carrier daily Key lt gt Select option shown Use default option Bio Plex Pro and Bio Plex Pro Wash Stations Rinsing Rinse Day Basic Operation and Maintenance The instrument should always be rinsed after use Afterwards the instrument can either be left switched on with the manifold in the rinsing solution or switched off after performing the correct maintenance the instrument will be left to stand for a short time i e up to 2 hours you may perform Rinse Day with wash buffer so that you do not have to prime before the next run Perform Rinse Day by making these menu choices Navigate to PROCEDURE in the primary menu and press OK PROCEDURE Use the keys under the and symbols to access the PROCEDURE menu if it is not already displayed Select and press OK for RINSE RINSE DAY Select and press OK for RINSE DAY Press OK when presented with the option to the left If presented with TIME you can use the default settings here as well Or navigate to VOLUME using lt gt Press OK when presented with the option to the left VOLUME 30uL Or use the default time settings x OK 9 CHANNEL 2 Select CHANNEL 1 or 2 using the choice keys lt gt EXIT CK Remember to prime channel 1 before performing the next wash protocol if you rinse with channel 2 Instrument will perform rinse procedure W
12. Press OK to continue to the next parameter of the selected program step Press OK to continue to the next parameter of the selected program step Press OK to continue to the next parameter of the selected program step Once all parameters of the step have been shown the selected Program Step appears again Press to continue to the next program step Press to return to the previous step Press OK to show the parameters of the final aspiration step If no final aspirate step was used CX CYCLEEND appears Press OK to view the cycle number Press END to exit the Show menu Press to return to the previous step Bio Plex Pro and Bio Plex Pro Wash Stations Defining New Programs Clear Program This submenu is used to clear a program from the instrument s memory Press OK to enter the CLEAR menu CLEAR lt gt EXIT OK CLR PROGR 1 A lt gt EXIT OK CLR PROGR 1 NO YES A DELETED the program is locked it cannot be cleared and the following message 15 displayed PROGRAM IS LOCKED The program can only be unlocked via the HydroControl software by users with the appropriate rights Select the program to be deleted from the instrument s memory Program Position Program Name Confirm that selected program is to be deleted CLR Program Position The name of the program which has been deleted appears Program Name DELETED Settings Menu The Settings menu has the follow
13. assays The instruments may also be used for the processing of ELISA assays and the washing of adherent cells Configurations Both wash stations are delivered with the magnetic carrier installed and the appropriate programs for performing the wash steps of magnetic bead based assays The Bio Plex Pro Il is also supplied with a vacuum manifold plate carrier to perform wash steps for standard Bio Plex assays and other non magnetic xMAP assays In order to do this you must install the vacuum manifold plate carrier and connect the appropriate tubing as indicated in Changing the Plate Carrier on page 23 You must also redefine the configuration of the instrument using the keypad as outlined in Complete Instrument Keypad Functions on page 43 Use the appropriate standard programs Both instruments can also be used to process other plate based assays If you wish to do so you can leave the magnetic carrier in place If you have concerns about the magnetic fields affecting your assay you can replace the magnetic plate carrier with the standard plate carrier Make sure you reconfigure the system as outlined in Complete Instrument Keypad Functions on page 43 Unauthorized modification of the instrument any of its options and or components as well as on any corresponding software or spare parts Will result in a loss of warranty and a potential loss in instrument performance It is important to understand that the proper installation of the ins
14. Reference Section Purpose of reference section Use this section of you have questions that are not answered in Basic Operation and Maintenance The reference section is provided for customers interested in more detail about the operation of the wash stations Provided you follow standard safety precautions this section is not required for normal operation There is some information in this section for troubleshooting and advanced programming options 30 Bio Plex Pro and Bio Plex Pro Wash Stations About the Instrument Instrument Details Instrument Safety Always follow basic safety precautions when using this product to reduce the risk of injury fire or electrical shock Read and understand all information in the IFU Failure to read understand and follow the instructions may result in damage to the product injury to operating personnel or poor instrument performance Observe all Warning and Caution notices see Warnings Cautions and Notes on page for a description of the notices used in this document Never open the Instrument while the instrument is plugged into a power source Observe proper laboratory safety precautions such as wearing protective clothing and using approved laboratory safety procedures Note Suitable Compact Microplates The instrument supports the processing of flat round or v shaped bottom microplates in 96 well format typically used for Enzyme Immunoassays EIA which conform to the
15. Socket Liquid Tubes Maximum filling level as indicated We suggest you place on botte bottles in location that allows clear view so that you can Bio Plex n nit li i J 3 easily monitor liquid levels Wash Buffer All Bottles should be placed on same level as instrument Bio Plex Pro and Bio Plex Pro 11 Wash Stations 15 Basic Operation and Maintenance Installing the Tubing Bracket Bio Plex Pro Il only Your Bio Plex Pro Il is shipped with the liquid hoses attached Attach the tubing bracket to the left side of the instrument as shown in the pictures below Location of Tubing Bracket Left side of instrument Bracket Bottom View Attach two screws A tool is provided for the installation of the bracket It is located in a bag containing a USB cable and cleaning pins for the manifold 16 Bio Plex Pro and Bio Plex Pro Il Wash Stations Basic Operation and Maintenance Selecting and Installing a Plate Carrier Magnetic Plate Carrier Vacuum Plate Carrier Version B Version A Attention Use only the type of magnetic carrier which arrived with your instrument If your wash station arrived with version B use of version A on your wash station will result in poor assays results Version B is back compatible with previously installed wash stations Use magnetic plate carrier with Use vacuum plate carrier with e Bio Plex Pro assays assays non ma
16. 20 C to 60 C 4 F to 140 20 to 8096 non condensing 10 to 80 non condensing Pollution Pollution degree Method of waste infectious waste Bio Plex Pro and Bio Plex Pro II Wash Stations 35 About the Instrument Description The diagram below shows the main components of the instrument Manifold Arm Manifold Keyboard and Display Unit Prime Tray Guide Bar Plate Support Liquid System Diagram Dispense Solenoid anifold Valve T1 Marked red Dispensing Pump Marked Aspirating Pump Channel select Solenoid Valves 36 Bio Plex Pro and Bio Plex Pro Il Wash Stations About the Instrument Bio Plex Pro Dimensions Tr L NI 3 3 o gt 1 j T wow TTTTUT 1 l 203 mm 7 99 I me a 08 275 2 10 83 OF v 8 996 Bio Plex Pro and Bio Plex Pro 1 Wash Stations 37 About the Instrument Bio Plex Pro Dimensions 09 uu 9 16 2 tT proce 7 7 oss mm 14 39 00 Z WWE LAL w o 3 b gt N an 38 Bio Plex Pro and Bio Plex Pro II Wash Stations About the Instrument Rear Panel Connections
17. Bio Plex Pro assays also appropriate for other standard and magnetic xMAP assays 20 user definable washing programs stored under program positions 1 to 20 Up to 60 processing steps per program each processing step can be identical or different from the previous step Adjustable soak time 1 second to 60 minutes and 59 seconds Variable shaking settings High Linear shaking with a shaking frequency of 25 Hz shaking amplitude of 1 mm Medium Linear shaking with a shaking frequency of 10 Hz shaking amplitude of 2mm Low Linear shaking with a shaking frequency of 5 Hz and shaking amplitude of 3 mm Adjustable dispense rate drip mode to 500 ul s Adjustable aspirating speed 1 to 3 Adjustable bottom positions bottom custom overflow Two rinse modes Rinse Day and Rinse Night can be used to select how the instrument is rinsed before it is left to stand or switched off Automatic microplate centering before starting any wash program Programmable strip selection before starting the washing procedure Bio Plex Pro and Bio Plex Pro II Wash Stations 43 Defining New Programs Alphabetical list of LCD text with explanations ASP RATE Aspiration rate ASPIRATE Aspirate BUBBLE ERROR Bubble error BUBBLE HIGH Bubble high BUBBLE MEDIUM Bubble medium BUBBLE SENSOR BURN IN TEST Burn in test CYCLE C Cycle CY CLEEND C Cycle End Le PH ASP Aspirate Cycl
18. Blot the bottom of the filter plate on a paper towel within 5 seconds after each program is completed If you are not sure how much time has elapsed run a PREVAC program to clear any residual buffer that may have wicked back into the wells before proceeding to the next step Vacuum protocols will not work if you do not have buffer in every well T Prime Procedure 2 CAUTION Do not run any programs until you have primed channel 1 Channel 1 if 1 Your last action was a rinse with Channel 2 2 The system has been idle for more than an hour 3 You are changing buffer in bottle 1 PROCEDURE VOLUME gt lt gt OK VOLUME 30 uL OK CHANNEL 1 al EXIT i Repeat for Channel 2 24 RUNT1 Program Wash Programs PROGRAM x gt OK START gt OK EXIT 555555555555 OK c Rinse Procedures Use RINSE DAY if you will use the instrument later that day Use RINSE NIGHT at the end of the day PROCEDURE z gt m gt RINSE lt gt EXIT OK I RINSE DAY lt gt EXIT VOLUME lt gt mE T VOLUME 30uL OK CHANNEL 2 EXIT or RINSE NIGHT gt Enable Disable vacuum carrier SETTINGS lt gt i OPTIONS P EXIT OK VACUUM FILTR EXIT
19. Chemical and Biological hazards can be associated with the waste material Microplate of processes run on the Instrument Treat the used Microplate Waste Bottle Prime tray on the Plate carrier disposables and all substances used in accordance with good laboratory practice guidelines Inquire about appropriate collecting points and approved methods of disposal in your country state or region Disposal of the Instrument Please contact your local service representative before disposing of the instrument Always disinfect the instrument before disposal Pollution degree 2 IEC EN 61010 1 Method of Disposal Contaminated Waste Bio Plex Pro and Bio Plex Pro 11 Wash Stations 73 Troubleshooting and Error Messages AY WARNING Depending on the applications parts of the Instrument may have been in contact with biohazardous material e Make sure to treat this material according to the applicable safety standards and regulations e Always clean and disinfect all parts before disposal Dispose of packaging material according to local regulations 74 Bio Plex Pro and Bio Plex Pro Wash Stations Troubleshooting and Error Messages Troubleshooting and Error Messages Errors Without Messages The following errors will not yield an error message from the instrument s firmware Error Description Possible Causes o e Display and LED dark Fuse is defective Contact your local Service Representative No o
20. If this occurs the manifold will need expensive repairs or will have to be replaced Bio Plex Pro and Bio Plex Pro 11 Wash Stations 65 Troubleshooting and Error Messages Cleaning the Manifold The manifold can be cleaned using supplied cleaning needles accessory box The small cleaning needle is for the dispensing needles and the large cleaning needle is for the aspirating needles Carefully push the cleaning needles into the aspirating and dispensing needles Rinse the manifold block with distilled water to ensure that all particles have been removed Agentle ultrasonic bath of warm distilled water for 15 minutes max five times manifold must be removed from the instrument e Autoclaving max 130 C max five times manifold must be removed from the instrument Do not autoclave manifolds with the Process Control option e Reinstall the manifold if necessary see page 66 e After the manifold has been cleaned switch the instrument on and perform the priming procedure using distilled water Cleaning Waste Bottles Before cleaning the waste bottles empty the bottles according to local disposal regulations The bottles must be cleaned regularly depending on the applications using a mild detergent WARNING When handling waste bottles it is advisable to adhere to applicable safety precautions including wearing powder free gloves safety glasses and protective clothing to avoid potential i
21. Tray Navigate to empty EMPTY PRIME TRAY in the Procedures and Press OK The prime tray will be aspirated Bio Plex Pro and Bio Plex Pro II Wash Stations 63 Troubleshooting and Error Messages Maintenance and Cleaning Cleaning Procedures WARNING All parts of the instrument that come into contact with potentially infectious material must be treated as potentially infectious areas It is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination when performing cleaning procedures and also when making adjustments to the instrument The most important cleaning procedure for this instrument 15 to rinse the liquid system with distilled water before the instrument is left to stand or switched off at the end of each day The manifold should be removed and cleaned thoroughly at least once every six months or whenever one or more of the needles are blocked Cleaning the Cover and Display The outer surface of the instrument and the display may be cleaned periodically using a tissue moistened with a mild detergent solution Caution Never use acetone as it will damage the covers Cleaning the Liquid System To clean the liquid system perform Rinse and Prime procedures as described in the Basic Operation and Maintenance section If the manifold is not rinsed the needles will become blocked
22. and clear all channels of an LLD setting Remember to exit to the Primary Menu before performing this function Press EXIT until the word EXIT disappears If this does not solve the problem please contact technical support SETTINGS Navigate to the settings option in the primary menu and press OK lt gt OK OPTIONS B EDIT LDD CHANNEL Select and press OK for EDIT LDD i EXIT OK CHANNEL CLR WASTE 1 Scroll through channels and press OK for any EXIT OK that display CLR CLR WASTE 1 SET WASTE 1 SET CHANNEL 1 EXIT OK Select and press OK for OPTIONS System clears LLD setting from selected channel System displays channel in new state Scroll through all channels to ensure that all channels display SET Press OK for all channels that display CLR When all channels are proceeded by the word SET press exit until you reach the Primary Menu Press EXIT until it disappears Make sure all channels are proceeded by the word Set WASTE CHANNEL 1 CHANNEL 2 78 Bio Plex Pro and Bio Plex Pro Wash Stations Declaration of Conformit TECAN AUSTRIA GMBH Untersbergstrasse A 5082 Salzburg Austria 43 G2 40 89 33 43 62 46 72 770 www tecar com x e Declaration of Conformity TECAN Austria GmbH herewith declare that the product identified as Product Type Microplate Was
23. as described on page 25 Check that the manifold arm is not blocked Check if the needles are catching on the side of the microplate use the Settings menu to adjust the positions for that particular type of microplate Sensor Defect Error RS485 Error 76 The following message error is displayed when the dispensing pump the dispensing sensor is defective SENSOR DEFECT OK Press OK to remove the error message and to return to the standby mode Make sure that drip mode has not been selected using distilled water Change the program parameters If the program parameters are correct and the error continues call the service technician This error is displayed if vacuum filtration is activated in the instrument settings on a Bio Plex Pro instrument A different message will be displayed on a Bio Plex Pro 11 5485 TIME OUT OK Press OK to remove the error message and to return to the standby mode Disable vacuum setting as outlined on page 24 The instrument cannot end the wash procedure Please end the test manually or dispose of the microplate Bio Plex Pro and Bio Plex Pro Wash Stations Troubleshooting and Error Messages PC Process Control System Error If the Process Control board does not respond the following error message is displayed Examples of Error Messages PC SYS ERROR PC FUNCT ERR PC ASP ERROR Process Control functionality has been inadvertently activated for your system Your sy
24. be aspirated Instrument state at the end of the wash 26 Considerations for leaving the instrument on or off after a rinse procedure e Leave the instrument on if you want to soak the manifold overnight Recommended during periods of heavy use Shut the instrument off if you do not wish to soak the manifold Rinse Night only Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance the instrument will be switched off at the end of operation the Rinse Night procedure should be performed This prevents the needles from becoming blocked Use the Rinse Night procedure to rinse the wash system as outlined above When the rinsing procedure is completed the following message is displayed RINSE NIGHT STOP END The manifold remains in the prime tray until END is pressed Press STOP to abort the procedure prime tray will not be aspirated The most important cleaning procedure for this instrument is rinsing the liquid system with distilled water at the end of each day Changing the Plate Carrier See Page 16 for detailed instructions on selecting and replacing the plate carrier Bio Plex Pro and Bio Plex Pro 11 Wash Stations 27 Basic Operation and Maintenance Preventive Maintenance Plan Weekly 28 WARNING All parts of the instrument that come into contact with potentially infectious material must be treated as potentially infectious areas It is advisable to adhere to applicab
25. be treated with care Z Position Overflow Z Position Custom Z Position Bottom JA Wash Position Diagrams MOVE OVER MOVE CUST 4 of Movement of manifold manifold i Custom Position EN Bottom Position E Movement Diagrams for Z positions with MOVE Overflow Position Bottom Position Bio Plex Pro and Bio Plex Pro 11 Wash Stations 49 Defining New Programs Aspirating Modes To improve wash efficiency and reduce the residual volume the manifold must be positioned so that the aspirating needles are correctly positioned in the wells for round bottom V shaped bottom or flat bottom well microplates Normal AspirationMode For round bottom or V shaped bottom wells the aspirating needles are placed in the middle of the wells Only one aspiration position can be selected Round Bottom Wells V Shaped Wells N N y b 4 N y 4 E N b 4 h N 4 Pp y N b d Normal Aspirating Positions Crosswise Aspiration Mode With flat bottom wells the instrument can perform crosswise aspiration using two aspiration positions per well The aspirating needles are set at two positio
26. filtration step or press or to select the next program step Program End or a new cycle 59 Defining New Programs Note Adjust parameters for filtration plates only when vacuum filtration is enabled Show Program This submenu 15 used to display the parameters that have been defined for a program An example wash program containing one cycle one wash step and a final aspiration step will be used to demonstrate this menu The displayed parameter settings cannot be altered in this submenu SHOW lt gt EXIT OK PROG1 EXAMPLE EXIT OK STRIP MODE EXIT OK 1 GRE96ft EXIT OK C1 CYCLE EXIT OK 1 WASH Fw EXIT OK POS OVERFLOW EXIT OK VOLUME 300yl EXIT OK CHANNEL 1 EXIT OK H SPEED 10mm s EXIT OK FL RATE 300ul s EXIT OK C1P1 WASH oog FIN ASPIRATE END OK 60 Press OK to enter the Show menu Select required program to show Press or to select programs from other positions Selected mode is displayed Selected plate type displayed Cycle 1 will be shown Press to show the program steps of this cycle Press OK to show the cycle number Press OK to show the parameters of the program step Press to continue to the next program step Press to return to the previous step Press OK to continue to the next parameter of the selected program step Press OK to continue to the next parameter of the selected program step
27. for magnetic assays Bio Rad Part Default for Magnetic Products 171 025001 Bio Plex Pro Yes flat bottom plates a complete list of suitable plates please contact Bio Rad technical support Only these recommended filter plates should be used with the Bio Plex Pro II when configured for standard Bio Plex or other polystyrene bead based xMAP assays Recommended microplates for vacuum filtration Vendor Manufacturer Part Default for Vacuum Products Millipore MSBVS1210 Yes If using other microplates you will have to enter new microplate definitions using the Hydrocontrol software included on the CD Please note the following requirements Parameters Characteristics Max overall microplate height 14 35 mm 0 76 mm 0 5650 inches 0 0299 inches Footprint 9 0 mm 0 3543 inches Pitch size center to center 9 0 mm 0 3543 inches Bottom shape Round V shaped and flat 12 Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance Installing the Instrument Unpack the instrument and install the instrument according to the instructions below Additional helpful information is located on the following pages e Unpacking checklist on page 40 Pro installation diagram on page 14 Bio Plex Pro Il installation diagram on page 15 Caution Do NOT turn the instrument on until you have completed the steps below Caution Before the instrument is installed and swi
28. party kits which require 200 ul washes Vacuum onl V 200 x2 or V 200 x3 For all protocols it is highly recommended that you wash the entire plate See page 18 for details Quick Guide Manual Steps Wash Station Protocols Vacuum Program Onl Dispense 125uL Assay Buffer PREVAC Perform Prewet Program Dispense 50uL Bead suspension MAG x2 Wash 2 cycles E X2 Dispense 50uL standars and solutions MAG x3 Incubate for 30 120 minutes Refer to assay manual or Wash 3 cycles VAC x3 Dispense 25uL detection antibody Inubate for 30 minute Wash 3 cycles Dispense 50uL SA PE Incubate for 10 minutes Wash 3 cycles Dispense 125uL Assay Buffer Place on shaker and shake for 1 minute Head on Bio Plex Reader Incubating reaction plates should be e At room temperature e Covered with aluminum foil or otherwise protected from light e Shaken at 900 1100 rpm for the first 30 seconds and at 300 rpm for the remainder of the incubation Bio Plex Pro and Bio Plex Pro II Wash Stations 23 Basic Operation and Maintenance Important Vacuum Protocol Precautions e Carefully place the plate so that it is flat and squarely seated between the metal frames on the vacuum carrier before stating a vacuum protocol may be necessary to apply a small amount of pressure to the plate for the first vacuum cycle of the day e Monitor your plate periodically to ensure complete removal of wash buffer before the next dispense cycle e
29. program position in which a new program will be defined or select an already existing program to edit Enter the required name for the program up to eight characters Select a character by moving it with the cursor keys between the commas and pressing the return key Select to delete a character Select 1 and press the return key to save the program name and continue to mode selection Press or to select between plate or strip mode Press or to select other plate types Press or to select the number of cycles cycles can be repeated up to 9 times Press or to select the process step ASP DISP WASH SOAK PROGRAM END CX CYCLE where X is the number of the next cycle Press OK to define the selected process step Once the process step has been defined the display appears with the step position incremented e g P1 becomes P2 Select or to define the next process step Press OK to define the selected process step To add another cycle press until CX CYCLE is displayed X is the number of the next cycle Press OK to define the number of cycles Press or and press OK to select PROGRAM END to finish defining the program Add a final aspiration step if necessary The parameters for the final aspirate step can be selected Once the program has been defined the Start menu appears again Bio Plex Pro and Bio Plex Pro Wash Stations Process Step Aspirate CX PX ASP EXIT OK Crossw A
30. the instrument using the on off switch in the rear panel of the instrument The initialization procedure is performed and depending on the instrument type the following initialization message is displayed BIO RAD BIO RAD BIO PLEX PRO BIO PLEX PRO Il If a Rinse procedure was aborted before the instrument was switched off the following message is displayed after the instrument initializes PLEASE RINSE OK Press OK and then select a Rinse procedure Rinse Day will work in all cases After the rinsing procedure has been performed the instrument proceeds to the standby mode and the following message is displayed The liquid system must be primed before it can be used Please ensure that the dispensing and aspiration pumps are not run for longer than a few minutes without liquid otherwise they will be damaged At the end of each working day perform the Rinse Night procedure with distilled water to ensure the proper performance of the instrument and to prevent needles from becoming blocked Bio Plex Pro and Bio Plex Pro Wash Stations Detailed Operating Instructions Complete Instrument Keypad Functions The instrument keypad 15 used to run and manage wash programs as well as vacuum filtration programs to define plate parameters adjust certain instrument settings and perform instrument procedures rinse prime and empty prime tray The following features are available Preloaded programs for processing Bio Plex and
31. trademarks mentioned in this document are used for identification purposes only and remain the exclusive property of their respective owners e is a registered trademark of Global Biotechnologies Inc Portland Maine USA e MagPlex and xMAP are trademarks of Luminex Corporation Austin TX USA Tween is a registered trademark of ICI Americas Inc Wilmington DE USA e Decon and Neutracon are registered trademarks of Decon Laboratories Limited Sussex UK e HydroControl is a trademark of Tecan Group Ltd Mannedorf Switzerland Bio Plex Pro and Bio Plex Pro 1 Wash Stations iii Bio Plex Pro and Bio Plex Pro II Wash Stations Table of Contents OP GON ple c EE V BASIC OPERATION AND MAINTENANCE 11 INSTRUMENT OPTIONS T 11 Meise uyzdaizeisizz cct 12 INSTALLING THE 13 BIO FIGX Fro Installation VIA TIS rca ha clan a o de DESEE C DE d UE 14 Bio Plex Pro Installation 15 Installing the Tubing Bracket Bio Plex Pro ll only sessi seen enne nnns 16 Selecting and Installing a Plate Carrier eie eese lice ee isse ean saa aaa Ra aaa e Ina NS ARR
32. E47 Wacker article code 21640582 the recommended concentration is 1 ml of antifoaming agent for 1 liter of waste solution Use antifoaming agents and concentrations as recommended by corresponding manufacturers Intended Use 32 The Bio Plex Pro and Bio Plex Pro ll are instruments for strip wise processing of microplates in the 96 well format The instrument is designed for professional use only and should only be operated by trained personnel The wash results obtained with the Instrument are influenced by the correct use of the instrument according to the instructions given in this document as well as the liquid compounds used reagents wash buffer chemical components The instructions for use storage and other handling in connection with samples or reagents must be strictly followed Before the instrument is installed and switched on it should be left to stand for at least three hours so there is no possibility of condensation causing a short circuit Before washing procedures are started make sure that the microplate position A1 is inserted correctly Bio Plex Pro and Bio Plex Pro Wash Stations About the Instrument The instrument is a general purpose laboratory instrument Europe and is a Class General Controls medical device U S for the processing of samples from biological and non biological origin The wash stations are designed primarily for the processing of Bio Plex and other
33. ETER MOVE CUSTOM MOVE MANIFOLD MOVE OVERFLOW MOVE TRANSPORT NAME NO ASP AT VACFIL PLATE NO PLATE FOUND PROGRAM Manifold broken Manifold detection Manifold up Missing parameter Move custom Move manifold Move overflow Move transport aspiration at vacuum filtration plate plate found PROGRAM FOUND No program found VAGFIL NO OF CYCLES NOT DEF OPTIONS OVERFLOW POS PARAMETER RANGE ASP ERR PC DISP ERR PC FUNCT ERR Plate PLATE INSERTED PLATE MODE PLEASE EMPTY PLEASE INSERT PLEASE RINSE POS 5 1 POS ASP2 46 vacuum filtration Number of cycles defined Overflow position Param eter range Process Control Aspiration error Process Control Dispense error Process Control Function error Plate Plate inserted Plate mode Please empty Please insert Please rinse Position Aspirate 1 Position Aspirate 2 POWERFAILURE Power failure PRIME EXIT YES Prime Exit Yes PRIME SOL OK Prime solution PROCEDURES PROCESS CONTROL Process Control Program deleted Rinse day Rinse solution RS485 Timeout fee SENSOR DEFECT set channel 1 Set channel 2 SET VAC WASTE Set vacuum waste SHAKE HIGH Shake SHAKE LOW shake medium SHAKE OFF Shake SHAKE YES shake Yes Bio Plex Pro and Bio Plex Pro Wash Stations Defining New Programs Time min 5 Use other name USER PROMPT
34. Instructions for Use for Bio Plex Pro amp Bio Plex Pro Wash Stations Revision B Attention Use only the magnetic carrier which arrived with your instrument See page 17 for more details Bio Plex Pro and Bio Plex Pro Wash Stations Notice Every effort has been made to avoid errors in text and diagrams however Bio Rad Laboratories assumes no responsibility for any errors that may appear in this publication Bio Rad reserves the right to change specifications at any time with appropriate validation verification and approvals Copyright Information The contents of this document are the property of Bio Rad Laboratories and are not to be copied reproduced or transferred to another person or persons without written permission Copyright 2008 Bio Rad Laboratories All rights reserved Declaration of Conformity See page 79 About the Instructions for Use IFU This document describes the use of the Bio Plex Wash Stations designed for performing hands free wash programs on Bio Plex and other xMAP assays It contains instruction for the use of the instrument and is intended as a reference for the user It contains information about the following e Installing the instrument e Operating the instrument e Programming of wash programs e Definition of wash parameters e Cleaning and maintenance procedures Trademarks The following product names and any registered or unregistered
35. Netherlands 0318 540656 New Zealand 0508 805 500 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 851 246 723 Spain 34 91 580 5200 Sweden 08 555 12700 Switzerland 061 7179555 Talwan 886 2 2578 7189 United Kingdom 020 8328 2000 708 Sig 0308
36. Plex Pro and Bio Plex Pro Wash Stations Detailed Operating Instructions The Bio Plex Pro instrument is shipped in one carton which contains the following important components 1 Standard plate carrier installed in instrument 2 Magnetic carrier 3 5 bag containing USB cable b 2cleaning needles 4 2liquid bottles 2 5 L with lids 5 1 waste bottle 5 with lid 6 Bundle of 2 tubes for connecting to the liquid bottles bmm outer diameter 3mm inner diameter 7 Main power cable 8 Instrument manual 9 CD with software and support material 10 10 Bio Plex Pro flat bottom plates The Bio Plex Pro Il is also shipped in one carton and contains everything from the above list plus 1 Located in the same small bag indicated in 3 above Not needed for Bio Plex Pro a Six small screws two screws are required to attach the Bio Plex Pro mounting bracket b AHex Tool 2 Avacuum plate carrier 3 Vacuum tubing mounting bracket see page 13 Instrument Waste Bottle Accessory Manuals Package Bio Plex Pro and Bio Plex Pro 11 Wash Stations 41 Detailed Operating Instructions Detailed Operating Instructions Switching the Instrument On 42 Ensure the instrument has been correctly installed and the main power cable is connected into the main power socket in the rear panel of the instrument and the liquid tubes are connected to the correct liquid and waste bottle s Switch ON
37. R daa Enn ARS 17 Changing the Vacuum CORNGUI ANON ase paw ee uita ae eet con t quta uta Vau Fu SE ie toute 18 THE comu ec ae te Seats Larisa a LAU 19 TURNING ON THE POWER me METER 19 KEYPAD BA ICO nino M nM MILL I E 20 PUIG Y IVA CIN mc 2 20 mius Tc 20 PRIMING THE INSTRUMENT ice olet usd t eI SO ORE 20 FANS TO E M EM UI 21 PLATE WASHING PROGRAMS coeno s Leccescanalandianscesaneates suaeneea A O 22 WHICH WASH PROGRAM DO TUSE ca e eU ss OIM PUDE Le TUM 23 QUICK 23 MM MM M MH Mc 24 mice m dca d 25 RINSE 25 RISENGA T 26 instrument state at the end the WASH usse cite odo dotes iene 26 ei eleaidsume lcd T 27 PREVENTIVE MAINTENANCE PLAN aoo vesci pu Ree A TE 28 aue et M ML IM E I MI LU MM bd 28 en beim dU DIM I MILIEU IM LUI IL MIDI E IM M M E MIS 28 zelo
38. The instrument has the following connections on the rear panel On Off Main Power Socket Nameplate shown enlarged below e USB WN Wo Lo L4 Vacuum Tubing Ports Bio Plex Pro Il onl USB port aa Waste Tube Instrument Name Plate The instrument name plate is located under the main power socket The company information on the top differs depending upon where it is purchased The nameplate also shows the model name the model part number and the serial number Use the provided power cord or a similarly rated replacement cord only Bio Rad Laboratories 6000 James Watson Drive Hercules CA 94547 Bio Plex Pro and Bio Plex Pro 1 Wash Stations 39 Detailed Operating Instructions Detailed Installation When installing moving or connecting the instrument follow the instructions in this publication Bio Rad Laboratories does not accept the responsibility for injury suffered by anyone attempting these operations without following the instructions in this publication nor for damage incurred to the instrument Make sure the laboratory meets all the requirements and conditions described in this chapter Required Working Area Select an instrument location that is flat level vibration free away from direct sunlight and free from dust solvents and acid vapors Allow at least 10 cm 4 in between the instrument and the wall or an
39. User prompt VAC CHAMB RIM S Vacuum chamber rinsing VAG NOT READY Vacuum not started Vacuum clean Vacuum filtration Vacuum prepare Vacuum filtration WASTE BOTTLE OK Waste bottle OK WASTEBOTTLE Waste bottle User Def User Defined POS BOTTOM POS Bottom POS CELL POS Cell POS CUSTOM POS Custom 7 5 OVERFLOW POS Overflow Z POS MOVE GL ST POS Move customer Z POS MOVE OVER POS Move overflow User Def User Defined Bio Plex Pro and Bio Plex Pro 11 Wash Stations 47 Defining New Programs Defining Custom Wash Programs The following instructions are for defining custom programs The programs needed for Bio Plex or other xMAP assays are pre installed on your wash station No further programs are needed unless your assay has unique needs Access the pre loaded programs as described on pages 18 and 19 The operating procedure is dependent on the instrument options and the program settings Be careful that the plates are positioned in the microplate reader as selected in the program otherwise spilling can occur and the instrument may become contaminated WARNING When the instrument is busy do not touch the manifold After the instrument has been used the manifold and prime tray may be infectious Wash Modes The instrument can wash a microplate using the following wash modes Plate Mode Each program step is performed on all of the selected strips o
40. ccording to national regional and local regulations WARNING All parts of the instrument that come into contact with potentially infectious material must be treated as potentially infectious It is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination when performing the disinfection procedure is very important that the instrument is thoroughly disinfected before it is removed from the laboratory or any servicing is performed on it Before the instrument is returned to the service center for service or repair it must be disinfected and a disinfection certificate completed by the operating authority If a disinfection certificate is not supplied the instrument may not be accepted by the service center or it may be held by the customs authorities Disinfection Solutions Use the following disinfection solutions for the disinfection procedure Decon 90 Decon Laboratories Limited e Decon Neutracon Decon Laboratories Limited e Microcide SQ Global Biotechnologies Ethanol WARNING Risk of fire and explosion Ethanol is flammable and when improperly handled can lead to explosions Proper laboratory safety precautions must be observed AN A Bio Plex Pro and Bio Plex Pro 11 Wash Stations 71 Troubleshooting and Error Messages Disinfection Procedure The instrum
41. e Cycle end CH PH CYCLE CYCLEEND DISP P Dispense PH SOAK Soak P USER PR P User Prompt VAC Vacuum WASH P Wash CH PRIMED Channa pimed Channa cleaning CHANNEL CLEAN NO CLEAN YES Cleaning Cleaning CLR CHANNEL 1 Clear channel 1 CLR CHANNEL 2 Clear channel 2 CLR CHANNEL 3 Clear channel 3 CLR CHANNEL 4 Clear channel 4 CLR VAC WASTE Clear vacuum waste CLR WASTE 1 Clear waste 1 Crossw Asp MO Crosswise Aspiration no Bio Plex Pro and Bio Plex Pro 11 Wash Stations Crossw Asp YES Crosswise Aspiration yes Cycle number Cycle number Define Edit Disable High VAG Disable high vacuum Disable Low VAC Disable low vacuum DISABLE Disable Process Control DISABLE Disable vacuum DISPENSE Dispense DISPENSE POS Dispense position Edit LLD channel Edit plates Empty bottles Empty prime tray Enable High VAC Enable high vacuum Enable Low VAG Enable low vacuum ENABLE PG Enable Process Control Enable vacuum Final aspiration ENABLE VAC FINAL ASP FINAL ASPIRATE Final aspiration FL RATE ul s Flow rate ul s FL RATE DRIP Flow rate Drip Hydro Process Control System error H SPEED mmis Head speed mm s Initialization error Invalid command Invalid parameter Liquid bottle OK 45 Defining New Programs MANIFOLD BROKEN MANIFOLD DETECT MANIFOLD UP MISS PARAM
42. e CX PX DISP EXIT OK Z POS OVERFLOW EXIT OK VOLUME 300 EXIT OK CHANNEL 1 EXIT OK FL RATE 300 ul s EXIT OK CX PY DISP EXIT OK 56 Select and confirm dispensing step DISP CX Cycle Number PX Program Step Number Select required dispensing position OVERFLOW CUSTOM MOVE OVER MOVE CUST or BOTTOM Select the volume of solution to be used 50 400 ul in increments of 50 ul Select the channel to be used 1 4 depending on instrument configuration Select required flow rate drip to 500ul sec After the process step has been defined the initial display appears CX Cycle Number PY Program Step Number incremented by 1 Press OK to define another dispense step or press or to select the next program step Program End or a new cycle Bio Plex Pro and Bio Plex Pro Wash Stations Dispensing Position Diagrams Z Position Bottom Z Position Custom EB lH Position Diagrams Overflow Position Bottom Position MOVE OVER t 2 Movement of manifold M Custom Position Bottom Position Movement Diagrams for Z positions with MOVE Bio Plex Pro and Bio Plex Pro 11 Wash Stations Defining New Programs Z Position Overflow MOVE CUST
43. ead to explosions Proper laboratory safety precautions must be observed viii Bio Plex Pro and Bio Plex Pro II Wash Stations Abbreviations A C CE cm CV ELISA EN F IEC IFU IVD IVD D PCR ppm QC REF SN torr TYPE USB VA WEEE Ampere Degrees Celsius Conformit Europ enne Centimeter Coefficient of Variation Enzyme Linked ImmunoSorbent Assay European Norm a voluntary European standard of the European Committee for Standardization or Comit Europ en de Normalisation CEN Degrees Fahrenheit International Electrotechnical Commission Instructions for Use Inch In Vitro Diagnostics In Vitro Diagnostics Directive Kilogram Liter Meter Millibar Milliliter Millimeter Millisiemens Microliter Process Control Polymerase Chain Reaction Parts Per Million Quality Control Reference Number Order Number Second Serial Number Tr ge Slow Blow Fuse Torr Millimeter of Mercury mmHg Name and Type of instrument Universal Serial Bus Volt Volt Ampere Waste Electrical and Electronic Equipment Bio Plex Pro and Bio Plex Pro II Wash Stations ix Basic Operation and Maintenance 10 Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance Basic Operation and Maintenance This section gives you instructions to configure install prime and use the Bio Plex Pro and Bio Plex Pro Il Wash Stations You can perform your first plate wash simpl
44. ed period of time Priming is performed to fill the liquid system of the instrument and to remove all air from the tubes A priming step must also be performed when switching to a different wash buffer Caution Do not use your new instrument until you have primed both channels 20 Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance Perform priming for both channels using the following procedure PROCEDURE Navigate to the Procedure in the primary OK menu and press OK Select and press OK for PRIME Confirm rinsing solution and tube in bottle PRIME SOLUTION OK lt gt YES WASTE BOTTLE OK lt gt YES VOLUME lt gt OK VOLUME CHANNEL 1 PROGRAM lt gt OK Repeat for channel 2 Confirm waste bottle is connected and sufficiently empty Press OK if you see VOLUME If you see TIME scroll down to VOLUME then press OK Press OK Select CHANNEL 1 using the choice keys lt gt Select OK and the prime procedure starts Instrument will perform the prime step and will then be ready to perform wash protocols Failure to Prime If you have a new instrument or it has not been used for long time the pump may fail to draw fluid from the liquid bottles This problem is easily solved 1 Disconnect the L1 tube from the bottle leaving it connected to the instrument 2 Hold the free end of the tube high and pipet
45. ent should be disinfected using one of the solutions mentioned previously WARNING Risk of fire and explosion Prior to cleaning the outer surface of the instrument and the display switch off the instrument and disconnect it from the main power supply Before starting the disinfection procedure use a rinse procedure Rinse Day with distilled or deionized water lab quality to flush the system WARNING The disinfection procedure should be performed in a well ventilated room by authorized trained personnel wearing disposable powder free gloves protective glasses and protective clothing Please note that the disinfectant can influence the performance of your instrument if it comes into contact with the electronics Wear protective powder free gloves protective glasses and protective clothing The following procedure should be used to disinfect the instrument 1 72 Prepare an autoclave bag for all disposables used during the disinfection procedure label it with autoclave tape and disinfect the material using the autoclave or follow the local regulations for decontamination of this material Prime the liquid system with disinfectant Perform the Rinse Night procedure with disinfectant solution and stop the procedure after five hours Switch off the instrument and disconnect the instrument from the power supply Disconnect the instrument from any accessories that are used for example Liquid Level Detec
46. f a microplate sequentially before proceeding to the next step The entire plate or the defined plate range is processed for the defined soaking time Strip Mode The entire wash program is performed on one strip of a microplate before proceeding to the next strip The strip is processed for the defined soaking time before proceeding to the next strip or group of strips 48 Bio Plex Pro and Bio Plex Pro Wash Stations Defining New Programs Washing Positions Wash dispense and aspirate steps can be defined in a wash program with the following Z positions Overflow For ELISA assays and cellular assays Overflow washing consists of a simultaneous aspiration and dispense step It creates a circular flow of wash buffer in the well and ensures that the topmost parts of the well are also washed Custom Any necessary washing position can be selected the custom positions are not saved as pdf files in comparison to the overflow and bottom position Move Overflow If Move Overflow MOVE OVER is selected the manifold moves step wise from the bottom position to the Overflow position during dispensing It is recommended for cell wash applications or any applications which must be treated with care Move Custom If Move Custom MOVE CUST is selected the manifold moves step wise from the bottom position to the user defined Custom Z position during dispensing It is recommended for cell wash applications or any applications which must
47. fold arm guard plate by removing the three screws using the Allen key provided gt screws 2 Liftthe manifold arm and remove the two screws that attach the manifold to the instrument using the Allen key provided 2 screws y 3 Carefully pull the tubing off the connectors the rear of the manifold and remove the manifold Bio Plex Pro and Bio Plex Pro 1 Wash Stations 69 Troubleshooting and Error Messages Installing the Manifold The standard manifolds are installed using the following procedure 1 Lift the manifold arm 2 Carefully fit the manifold onto the manifold arm and ensure that the locating pins are correctly inserted through the holes in the bracket Locating Pin Locating Pin 2 screws 3 Tighten the manifold into place using the two screws using the Allen key provided 4 Fitthe dispensing tubes marked blue onto the top connector on the rear of the manifold 5 Fitthe aspirating tubes marked red onto the two bottom connectors on the rear of the manifold marked with red labels 6 Attach the black manifold guard plate with the 3 screws using the Allen key provided 3 screws 43 i 7 Lower the manifold arm and prime the instrument before starting a wash program 70 Bio Plex Pro and Bio Plex Pro Il Wash Stations Troubleshooting and Error Messages Instrument Disinfection AY WARNING The disinfection procedure should be performed a
48. gnetic Bio Plex Precision Pro assays e Any assay on Bio Plex COOH beads e Any assay with MagPlex e Any assay on MicroPlex microspheres microspheres Remove the standard ELISA plate 1 Make sure the instrument is turned off 2 Lift the manifold arm 3 Slightly tilt the plate carrier towards the right mm KE 4 Carefully slide the plate carrier toward the front of the instrument until it clears the guide bar lt Guide Bar BA A A AN Installing the plate carrier is the reverse of the procedure above 1 Carefully guide the plate carrier onto the guide bar in a slightly tilted position as shown above 2 Push the plate carrier completely into the instrument so that the front of the carrier is even with or past the Bio Rad logo 3 Lower the plate carrier 4 Lower the manifold arm Bio Plex Pro and Bio Plex Pro 11 Wash Stations 17 Basic Operation and Maintenance Changing the Vacuum Configuration You must set the vacuum system appropriately for the plate carrier used This is done through the VACUUM FILTR menu Follow the procedure shown below to e Enable Low VAC when using the vacuum carrier e Disable Low VAC when 1 using the magnetic carrier 2 using the standard ELISA plate carrier Disabling Enabling the Vacuum Carrier Go through these menus to disable or enable the vacuum carrier If you are disabling the vacuum the menus will dis
49. h Station Model Designation Bio Plex Pro Bio Plex Pro Article Number s 30034376 30034377 Placed Into market by Bio Rad Laboratories 6000 Alfred Mobel Drive Hercules 04547 Produced by Tecan Austria Untersbergstr 1A 5082 Grodig Austria is in conformity with the provisions of the following EC Directive s when installed in accordance with the installation instructions contained in the product documentation 2006 95 EC Low Voltage Directive 2004 108 EC EMC Directive and that the standards referenced below were taken in consideration EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Fart 1 General requirements 61010 2 D81 2002 Safety requirements for electrical equipment for measurement control and A1 2003 laboratory use Part 2 081 Particular requirements for automatic and sami automatic laboratory equipment for analysis and other purposes 61326 1997 Electrical Equipment for Measurement Control and Laboratory Use EMC 1 1998 2 2001 Requirements A3 2003 Year of CE marking 08 Place Date Gr dig 2008 03 03 Legally binding signature Harald Negele Function Head of Finance and Administartion Head of Sales and Marketing RA 7001584 Declarabon of Conformity V D3 Effective Date 2007 15 07 Page 1 of 1 Bio Plex Pro and Bio Plex Pro 1 Wash Stations 79 Index 50
50. hen the rinsing procedure is complete the following message is displayed RINSE DAY SIOP END The manifold remains in the prime tray until END is pressed Press STOP to abort the procedure prime tray will not be aspirated Bio Plex Pro and Bio Plex Pro 11 Wash Stations 25 Basic Operation and Maintenance Rinse Night the instrument will be left to stand for a longer period of time Such as overnight perform the Rinse Night procedure using these menu choices PROCEDURE VOLUME lt gt OK VOLUME 30 uL Es CHANNEL 2 EXIT OK Navigate to PROCEDURE in the primary menu and press OK Use the keys under the lt and gt symbols to access the PROCEDURE menu if it is not already displayed Select and press OK for RINSE Select and press OK for RINSE NIGHT Press OK when presented with the option to the left If presented with TIME you can use the default settings here as well Or navigate to VOLUME using lt and gt Press OK when presented with the option to the left Or use the default time settings Select CHANNEL 2 using the choice keys lt gt At the end of the day always rinse with CHANNEL 2 distilled or equivalent Instrument will perform rinse procedure When the rinsing procedure is completed the following message is displayed RINSE NIGHT STOP END The manifold remains in the prime tray until END is pressed Press STOP to abort the procedure prime tray will not
51. ing options Edit Plates Adjustment procedure for setting the parameters for up to Options Bubble Sensor 20 different microplates The Settings menu contains the following submenus Edit LLD Channels Vacuum Filtration Process Control The Bubble Sensor sensitivity can be set to Low Medium or High depending on liquids used if this option is installed If very foamy liquids are used the bubble sensor should be switched OFF The Settings menu has the following structure SETTINGS gt BUBBLE SENSOR lt gt EXIT OK OPTIONS lt gt EXIT OK EDIT PLATES lt gt EXIT OK Bio Plex Pro and Bio Plex Pro II Wash Stations 61 Defining New Programs Edit Plates Predefined Bio Plex plate definitions should not be edited EDIT PLATES Plate 1 GRE96ft Press or to select the plate 1 20 and press OK to edit the plate parameters Press OK to continue OVERFLOW POS Use the cursor keys to select the position to be defined lt gt EXIT OK OVERFLOW BOTTOM ASP1 or ASP2 and press OK For round and V shaped bottom plates only one aspiration can be defined Z User Def 14600 The instrument moves to the predefined position f EXIT OK Use the and keys to change the position Confirm with OK H The next position can then be selected Press OK to save the defined position Press EXIT to leave the menu without saving the position The inst
52. le safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination when performing cleaning procedures and also when making adjustments to the instrument WARNING RISK OF FIRE AND EXPLOSION Prior to cleaning the outer surface of the instrument and the display switch off the instrument and disconnect it from the main power supply Prime the liquid system if necessary Perform Rinse Day with wash buffer if the instrument is left to stand for a short time up to 2 hours Use distilled water if you anticipate longer intervals between uses Perform Rinse Night with distilled water at the end of a day of use If necessary due to particles soiling etc prime the instrument several times with distilled water If the instrument will be left to stand for a longer period of time more than one week prime with distilled water and then prime without liquid Perform Rinse Night with distilled water and soak the manifold overnight leave instrument on Prime the instrument without liquid to empty the entire liquid system Reconnect the lines and prime with distilled water unless not using the instrument for an extended period Check the filter s in the liquid bottle s for particles and rinse liquid filter s with distilled water Clean the plate carrier guide bar with 70 ethanol Bio Plex Pro and Bio Plex Pro Wash Stations Basic Ope
53. ndicated on the bottle to avoid potential overflow The contents of waste bottle are potentially infectious so it is important to wear protective clothing gloves lab coat and safety glasses when emptying OR handling a waste bottle Bio Plex Pro and Bio Plex Pro 11 Wash Stations 67 Troubleshooting and Error Messages Replacing the Manifold The Instrument is delivered with the manifold already installed The manifold drawing below shows the basic components Dispensing Tubes Locating Pin Locating Pin Aspirating Tubes The Rear of the Manifold Note Always wear powder free gloves when handling the manifold WARNING After the instrument has been used the manifold may be infectious Before the manifold is removed it must be thoroughly disinfected It is advisable to adhere to applicable safety precautions including the wearing of powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination WARNING Prime the instrument without liquid to empty all liquid from the system before removing the manifold 68 Bio Plex Pro and Bio Plex Pro Wash Stations Troubleshooting and Error Messages Removing the Manifold WARNING Prime without liquid to remove liquids from the tubing system The manifold should be removed and thoroughly cleaned at least once every 6 months or whenever needles become blocked 1 Lift the manifold arm and remove the black mani
54. nfectious disease contamination 66 Bio Plex Pro and Bio Plex Pro Wash Stations Troubleshooting and Error Messages Liquid or Foam Spills WARNING Always switch off the instrument before removing any kind of spills on the Instrument All spills liquid or foam must be treated as potentially infectious Therefore always adhere to applicable safety precautions ncluding wearing powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination Additionally all resulting waste from the clean up must be treated as potentially infectious and the disposal must be performed according to local regulations If the spill occurs in the instrument a service technician 15 required Spilling of liquid or foam may occur when the Instrument is operated improperly such as Microplate used does not match installed manifold The strip plate positions do not match the positions defined in the program used for processing Plate parameters are not properly adjusted Waste bottle not emptied when liquid level or foam level reaches maximum filling level No anti foaming agent used with wash buffers showing strong tendency to foam Always remove spills immediately after they have occurred Use paper tissue to soak up spills and wipe surface dry WARNING WASTE BOTTLE LIQUID LEVEL Make sure that the liquid level of the waste bottle is always kept below the maximum level i
55. ng Cover and Di SO AY 65 1 65 CENA TAI NON 66 BOW CS 66 MEET ETT 67 PE AGING IERIE OLD m 68 FACTO WING Me VIA OIG 69 FIC WAIN ONO c Ms 70 INSTRUMENT DISINFECTION 71 Bites ees MT EE 71 OIE a ele gin gd 016 16 0 T 72 DISPOSAL OF INSTRUMENT T Disposal of Operating 73 Disposal Of the 73 TROUBLESHOOTING AND ERROR 5 75 ERRORS WITHOUT IES SAG 75 ROR IMS SG caches cect cece teed ede ete oe pee tes So deeded nema LM M RUN acess 75 75 VOC IO TOM m 75 75 MOTTATTE T OF acs 76 2an DE ET ETTO S 76 O
56. ns Process Step Soak CX PX SOAK EXIT OK TIME 0 min 10 8 Chg SHAKE OFF EXIT OK CX PY SOAK EXIT OK Process Step User Prompt CX PX USER PR EXIT OK Process Step Vacuum Filtration CX PX VAC EXIT OK VAC 100 0 mBar EXIT OK TIME 0 min 10 8 Chg CX PX ASP EXIT OK Bio Plex Pro and Bio Plex Pro II Wash Stations Defining New Programs Select and confirm soak step SOAK CX Cycle Number PX Program Step Number Select required soaking time using keys 0 60 min 1 59 seconds Press Chg to select minutes or seconds The shaking speed can be selected OFF LOW MEDIUM or HIGH After the process step has been defined the initial display appears CX Cycle Number PY Program Step Number incremented by 1 Press OK to define another soak step or press or to select the next program step Program End or a new cycle Select and confirm User Prompt strip on required position in current program Select and confirm Vacuum Filtration step VAC CX Cycle Number PX Program Step Number Select required vacuum pressure using the keys Select required soaking time using the keys 0 60 min 1 59 seconds Press Chg to select minutes or seconds After the process step has been defined the initial display appears CX Cycle Number PY Program Step Number incremented by 1 Press OK to define another vacuum
57. ns on the bottom of the wells front edge and back edge Aspirate Y Offset 1 Aspirate Y Offset 2 Front edge Back edge Crosswise Aspiration for Flat Bottom Wells 50 Bio Plex Pro and Bio Plex Pro Wash Stations Defining New Programs Drip Mode The slowest dispensing mode is the drip mode This mode 15 not recommended for xMap or Bio Plex applications Drip mode is used for very sensitive applications e g washing cell layers because the instrument offers the possibility to dispense liquid in small drops This minimizes the detachment of cells and increases the remaining cell percentage at the bottom of the wells The following example shows a typical wash program for working with adherent cells as it would appear in the Define Edit menu See Define Edit a Program on page 49 for further information about defining programs Note The following example program should not be used as a standard wash procedure for cell washing as it is necessary to adjust the wash parameters such as Z position MOVE CUST dispensing and aspirating rates head speed etc according to the cell type used Bio Plex Pro and Bio Plex Pro 11 Wash Stations 51 Defining New Programs DEFINE EDIT lt gt EXIT OK PROGR 1 EXIT OK NAME CELLWASH 567890 PLATE MODE
58. play Disable in place of Enable SETTINGS Navigate to Settings in the Primary menu Failure to set and press OK Vacuum Filtration lt gt OK settings appropriately for the installed plate OPTIONS Select and press OK for OPTIONS carrier will result in a MANIFOLD HEAD UP error This indicates that the manifold arm cannot VACUUM FILTR Select and press OK for VACUUM FILTR lower appropriately EXIT OK Enable Low VAC Enable vacuum carrier by choosing YES EXIT YES Disable Low VAC will be displayed if already enabled Enable Low VAC EDIT LLD CHANNEL System enables vacuum carrier System places you in the Options menu at EDIT LLD CHANNEL EXIT to Primary menu press EXIT until it is no longer an option PROGRAM gt The instrument must be turned off and then back on for change to take effect 18 Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance Installing the Microplate Insert the 96 well microplate into the plate transport for washing and ensure that the microplate is correctly oriented position of the plate corresponds to position 1 marked on the plate transport If using the vacuum plate carrier for polystyrene based assays see Changing the Plate Carrier on page 23 4 42 9 4 e es 60060 2 0000000 00000
59. r incorrect dispensing Dispensing needles blocked Cleaning the Manifold on page 62 No or incorrect aspirating Aspirating needles blocked Cleaning the Manifold on page 62 Error Messages Plate Error If the plate sensor does not recognize the plate or no plate is inserted on the plate carrier the following message is displayed NO PLATE OK Press OK and insert the plate correctly on the plate carrier Program Too Big Error If the program has been defined with more than 60 process steps each cycle is worth 2 steps the following message is displayed PROGR TOO BIG OK Press OK and define a program with less than 60 process steps Transport Error If the instrument cannot move the plate carrier the following message is displayed INIT ERROR OK Press OK to remove the error message and the instrument returns to the standby mode Check that the plate carrier is correctly inserted and that the plate carrier system is clear Bio Plex Pro and Bio Plex Pro II Wash Stations 75 Troubleshooting and Error Messages Manifold Arm Error If the manifold arm cannot be lowered correctly due to obstruction of its normal movement the following message is displayed MANIFOLD HEAD UP EXIT Press EXIT to remove the error message and to return to the standby mode Check that the microplate is correctly inserted into the plate carrier Check that the VACUUM FILTR setting is set appropriately for the plate carrier being used
60. ration and Maintenance Every Six Months e Clean the plate carrier guide bar with 70 ethanol e Remove the magnetic plate carrier and check the spring loaded centering mechanism at the right rear of the carrier If the mechanism does not move freely rinse thoroughly with 70 ethanol e Clean aspiration and dispensing needles Two kinds of cleaning needles are supplied with the instrument Clean the manifold aspirating needles with the smaller of the two Clean the dispensing needles with the thicker cleaning needles Carefully raise the manifold arm and clean each instrument needle by inserting the cleaning needle into the instrument needle and gently agitating to remove any possible debris Then rinse with water f you choose to remove the manifold for cleaning refer to page 66 for instructions As Needed To replace the plate carrier seal 1 Ifthe plate carrier seal becomes visibly damaged remove the defective seal and clean the plate carrier frame with ethanol making sure no adhesive is left 2 Peel back the protective paper from the new seal and attach to the plate carrier frame be careful not to block the ventilation hole Note Clean the aspirating and dispensing needles periodically or immediately if they become clogged with particles or crystals Yearly Service Engineer Required Yearly maintenance is performed by the service engineer Bio Plex Pro and Bio Plex Pro 11 Wash Stations 29 About the Instrument
61. rument moves to the home position and the firmware returns to the Edit Plates menu EDIT PLATES Options Submenu The Options submenu has the following structure OPTIONS lt gt OK EDIT LLD CHANNEL EXIT OK VACUUMFILTRATION PROCESS CONTROL Vacuum Filtration See Page 18 Enabling Disabling Vacuum Note Make sure that the vacuum transport is mounted when the vacuum filtration option is enabled 62 Bio Plex Pro and Bio Plex Pro Wash Stations Defining New Programs Bubble Sensor It is recommended that the Bubble Sensor be left off BUBBLE SENSOR Press OK to continue BUBBLE OFF Press or to select between OFF LOW MEDIUM and HIGH EXIT Press OK to confirm Procedures Menu The Procedures menu has the following options Start rinse procedures Prime Start prime procedures Empty Prime Tray Aspirate liquid out of the prime tray Vacuum Filtration Prepare the vacuum pressure to prevent delays during the running of a wash program The Procedures menu has the following structure PROCEDURES lt gt OK RINSE lt gt EXIT OK VACUUM FILTRATION lt gt EXIT OK PRIME EMPTY PRIME TRAY lt gt EXIT OK lt gt EXIT OK For details see the Rinse section on page 24 Rinse Prime For details see Priming the Instrument on page 20 Empty Prime
62. sp YES EXIT OK POS BOTTOM 4 H SPEED10mm s ASP RATE 2 CX PY ASP EXIT OK Note Defining New Programs Select and confirm aspirating step ASP CX Cycle Number PX Program Step Number Select YES using or to perform crosswise aspiration or select NO to perform normal aspiration Press or to select the aspiration position BOTTOM OVERFLOW or CUSTOM Select required aspirating time 1 20 seconds Select required aspirating speed 1 20 mm sec Select required aspirating rate 1 3 where 1 is the weakest and 3 the strongest rate After the process step has been defined the initial display appears CX Cycle Number PY Program Step Number incremented by 1 Press OK to define another aspiration step or press or to select the next program step Program End or a new cycle Low residual volumes cannot be guaranteed if not all wells in a strip are filled with liquid To prevent cross contamination the head speed should be lowered Bio Plex Pro and Bio Plex Pro 11 Wash Stations 55 Defining New Programs Aspiration Position Diagrams Position Bottom Position Custom Position Overflow Baan Aspiration Position Diagrams Process Step Dispens
63. standard defined by the Society of Biomolecular Screening However it is recommended that you only use the recommended plates for assays WARNING Waste bottle Liquid level Make sure the liquid level of the waste bottle is always kept below the maximum level indicated on the bottle to avoid overflow As the contents of the waste bottle are potentially infectious wear protective clothing gloves lab coat and safety glasses when emptying handling a waste bottle Inquire about appropriate collecting points and approved methods of disposal in your country state or region Bio Plex Pro and Bio Plex Pro 11 Wash Stations 31 About the Instrument WARNING When using wash buffers that show a strong tendency to foam empty the waste bottle as soon as the foam level has reached the maximum filling level indicated on the waste bottle Add a commercially available anti foaming agent Such as silicone oil to the empty waste bottle to reduce foaming If foaming continues to be a problem we recommend switching to a larger waste bottle not provided with the Instrument and to increase the concentration of anti foaming agent in the waste bottle To help facilitate breakdown of the foam in the waste bottle carefully swirl the waste bottle from time to time to improve mixing between foam layer and anti foaming agent Refill anti foaming agent after emptying waste bottle For example when using the Wacker Anti Foam Emulsion S
64. stem is not equipped for process control so it must be deactivated The following directions describe how to check and disable a PC setting Remember to exit to the Primary Menu before performing this procedure Press Exit until the word EXIT disappears SETTINGS Navigate to the settings option in the j primary menu and press OK gt OK OPTIONS PROCESS CONTROL Select and press OK for PROCESS l EXIT OK CONTROL DISABLE PC lf PC is enabled the screen should display EXIT OK DISABLE PC Press OK DISABLE PC EDIT LLD CHANNEL EXIT OK PROGRAM lt gt OK Turn instrument off and back on for change to take effect If the error continues call the service technician Select and press OK for OPTIONS system clears PC setting System places you in the options menu at EDIT LLD CHANNEL EXIT to primary menu unit EXIT disappears No Aspiration at Vacuum Filtration Error The following error message appears on the display NO ASP AT VACFIL OK Press OK to remove the error message and to return to the standby menu Bio Plex Pro and Bio Plex Pro II Wash Stations 77 Troubleshooting and Error Messages LLD Errors Examples ERROR ERROR LLD has been inadvertently activated for one channel in your system This is an advanced function for which your system is not equipped All channels should be deactivated for LLD The following directions describe how to check
65. tandards Variable Volume of solution 50 3000 in 50 ul increments for washing dispensed 50 400 ul in 50 ul increments for dispensing Dispensing accuracy lt 2 Measured under following conditions 8 way manifold 300 ul dispensing rate 3 250 ul s well wash buffer Bio Rad 96 well flat bottom plates Cat 171025001 Dispensing uniformity lt 4 CV Measured under following conditions 8 way manifold 300 ul dispensing rate 3 250 ul s well wash buffer Bio Rad 96 well flat bottom plates Cat 171025001 Residual volume Measured under following conditions lt 4 ul well using Bio Plex Pro flat bottom plates and the recommended preloaded programs Optimized for high bead recovery and low assay 34 Bio Plex Pro and Bio Plex Pro Wash Stations About the Instrument PARAMETERS CHARACTERISTICS Carry over lt 1 ppm between wells The carry over between wells lt 1 ppm cannot be guaranteed if not all wells are filled with EE UU UO Vacuum Filtration Vacuum range Bio 50 to 150 absolute pressure Plex Pro II Only Power Supply _ 100 120 V or 220 240 V 50 60 Hz Outside dimensions Width 275 mm Depth 366 mm Height 180 5 mm Width 10 8 in Depth 14 4 in Height 7 1 in without Vacuum Filtration Greater depth and height in service position Weight without Vacuum Filtration pee asesino NR 15 C to 35 C 59 F to 95
66. tched on it should be left to stand for at least three hours so there is no possibility of condensation causing a short circuit 1 Ensure that the on off switch in the rear panel of the instrument is in the off position 2 Connect the solution tubes L1 and L2 to the inlet connectors on the rear panel of the instrument See the diagrams on the following pages Connect the solution tubes to the corresponding liquid bottles Connect the waste tube to the waste bottle do not kink the tubes IF your instrument is a Bio Plex Pro install the tubing bracket as shown page 16 Fill Liquid 1 bottle with Bio Plex wash buffer Fill Liquid 2 bottle with distilled or higher grade water 8 Remove the standard ELISA carrier and install the appropriate plate carrier based on the assays you will be running Selecting and Installing a Plate Carrier on page 17 Bio Plex Pro and Bio Plex Pro 11 Wash Stations 13 Basic Operation and Maintenance Bio Plex Pro Installation Diagram 14 ON OFF Switch Main Power Socket We suggest you place bottles in location that allows clear view so that you can Bio Plex Distilled easily monitor liquid levels Wash H 0 Buffer Maximum filling level as indicated on bottle All Bottles should be placed on same level as instrument Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance Bio Plex Pro Installation Diagram ON OFF Switch Main Power
67. te 3 4 ml of deionized water to the tube while performing the prime procedure in channel 1 as described above Alternately you may carefully use a syringe to inject 1 2 ml of water directly into the L1 port of the instrument reconnect the tubing and run a prime procedure Bio Plex Pro and Bio Plex Pro II Wash Stations 21 Basic Operation and Maintenance Plate Washing Programs Instructions for running the preconfigured wash program are detailed below Instructions for setting new Wash Dispense and Aspirate modes into a new wash program and instrument rinse instructions are found in the Defining New Programs section on page 53 PROGRAM OK RUN1 Program X lt gt EXIT OK SSSSSSSSSSSS OK 22 Navigate to PROGRAM in the primary menu and press OK Use the keys under the lt and gt symbols to access the Program menu if it is not already displayed Press OK Select the required program and press OK to continue Choose OK Rows are represented by S selected or N not selected All rows should be selected It is highly recommended that all wells are selected and the entire plate is washed Bio Plex Pro and Bio Plex Pro Wash Stations Basic Operation and Maintenance Which Wash Program Do I Use In this instance Use Wash Station Program First wash magnetic assa MAG x2 All other washes magnetic assa MAG x3 PREVAC VAC x All other washes vacuum assa VAC x3 Third
68. tion System computer etc Accessories that should be shipped together with the instrument have to be included in the disinfection procedure Carefully spray the disinfectant solution on all outer instrument surfaces or wipe with a disposable soft tissue paper towel soaked in the disinfectant After a minimum contact time of 10 minutes repeat step 5 of this procedure Wipe the outer surfaces of the instrument dry Pack the instrument and its accessories Wash your hands with a mild detergent and then disinfect them Bio Plex Pro and Bio Plex Pro Wash Stations Troubleshooting and Error Messages 10 If returning the wash station to Bio Rad for servicing contact your local Bio Rad service office to obtain the appropriate documents Failure to do so may result in significant delays Disposal of Instrument This chapter gives instructions on how to lawfully dispose of waste material accumulating in connection with the instrument Observe all federal state and local environmental regulations ATTENTION European Directive 2002 96 EC on waste electrical and electronic equipment WEEE has disposal rules which attempt to avoid negative environmental impacts associated with the treatment of electrical and electronic equipment waste Do not treat electrical and electronic equipment as unsorted municipal wasie Collect waste from electrical and electronic equipment separately Disposal of Operating Material WARNING
69. trument and software alone will not ensure compliance with national regional or local regulations A range of policies and standard operating procedures according to applicable regulations must additionally be established Caution Mandatory System Validation by Operating Authority The instrument has been validated on representative Enzyme Immunoassays only in Europe It is therefore the responsibility of any Operating Authority to ensure that the instrument has been validated according to applicable regulations for each specific assay used on the instrument Bio Plex Pro and Bio Plex Pro 11 Wash Stations 33 About the Instrument Instrument Specifications Note Instrument specifications have been defined using Bio Plex Pro flat bottom microplates Cat 171025001 and may vary using other 96 well plates The table below lists the specifications for the instrument All specifications listed below are based on the following instrument settings Dispense Wash rate 250 ul s well Aspiration rate 3 PARAMETERS CHARACTERISTICS Display unit Liquid Crystal Display with 2 rows of 16 digits Keyboard 0 4 4 membrane keyboard 0 membrane keyboard c NN of dispensing M to 4 channels channels Manifold Manifoldtypes 8 8 channel manifold manifold Interface All connected devices must be approved and listed as per 60950 1 Information Equipment Safety or equivalent local s
70. y by using these instructions and the onboard wash program This section also contains references to more detailed information throughout this manual Instrument Options The Bio Plex Pro and Bio Plex Pro Il Wash Stations are designed for strip wise washing of 96 well microplates They are designed primarily for the processing of Bio Plex and other xMAP assays The wash station is available in two versions Wash Configuration Bio Plex Pro Bio Plex Pro Il Vacuum filtration Yes Magnetic separation Both wash stations are delivered with the standard carrier installed and the appropriate programs for performing your assays Replace the standard carrier with the appropriate carrier for your assays as outlined on page 17 Which Wash Program Do Use On page 23 describes the standard wash programs necessary for the different assays To run standard ELISAs or other plate based assays you must modify programs and plate definitions using the HydroControl M software available on the CD that ships with both instruments This software is not needed for Bio Plex or Bio Plex Pro assay processing Follow the instructions in Defining New Programs on page 53 Bio Plex Pro and Bio Plex Pro II Wash Stations 11 Basic Operation and Maintenance Microplate Requirements Use recommended microplates If you use different microplates your assay results may not be reproducible and you may experience excessive bead 1055 Recommended microplates
71. y other equipment Do not place any items close to the instrument that could obstruct airflow Ideally the liquid bottles should be placed at the same level as the instrument and in plain sight so they can be easily monitored for liquid level They should not be placed more than 1 m below the level of the instrument since this may result in instrument malfunction For information regarding outer dimensions and weight of the instrument see the Instrument Specifications section on page 30 Power Requirements The instrument is designed to operate at either 100 120 V or 220 240 V No voltage setting is required as the instrument automatically senses the supplied voltage Unpacking and Inspection Checklists 40 Visually inspect the container s for damage before opening Report any damage immediately Place the carton in an upright position and open it Lift the instrument out of the carton and place it in the selected location Remove the transport protection from the instrument and manifold Visually inspect the instrument for loose bent or broken parts Report any damage immediately 7 Compare the serial number on the rear panel of the instrument with the serial number on the packing slip Report any discrepancy immediately 8 Check the instrument accessories against the packing list Save packing materials for further transportation purposes The instrument must be returned in the original packaging ONS Bio

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Bio-Plex Pro™ & Bio-Plex Pro II Wash Stations