Page 1 of 1 Ceramospher (Chiral) 17.02.2014 http://hplc.shiseido.co
Contents
1. Ceramospher Chiral Page 1 of 1 SHLSEIDO Products Ceramospher Chiral List of Sales Technical Materials Rep resentatives E E Overview User s Manual A Contact Shiseido Catalog List Ceramospher is coined from ceramic and sphere This is a completely new type of column for optical separation where the optically active metal complex A Ru phen 3 phen 1 10 phenanthroline is adsorbed 0 porous spherical ceramics by cation exchange Structure of synthesized magnesium The clay mineral used for the and sodium silicate i s php em chromatographic support has a multilayer structure Analyte molecules o Mp are supposed to enter narrow gaps between the layers and to fit into environments where three dimensional interactions for chiral recognition take A Ruiphen Sim lon exchange place When trying many chiral compounds probability of successful optical separation is much higher than those of conventional chiral columns Difference between RU 1 and RU 2 RU 2 packing material is created from RU 1 by further special hydrophobic treatment Consequently Ceramospher Chiral RU 2 allows the use of aqueous reversed phase mobile phases Features Excellent separation hit rate Optimum choice particularly for the optical separation of neutral and acidic compounds Optical separation list No need to optimize conditions Analytical conditions can be easily set by using a2. an V liquid may leak because of inadequate ferrule adhesion Therefore we recommend replacing the ferrule together with the column Tf the column is replaced frequently the male nut may crush the ferrule and liquid may leak Since tightening the nut too much may cause its head to come off replace the ferrule at an early Stage End fitting Ferrule 18 hale nut alee Tube 1 16 Filter Fig 1 Column connection PAGE TOP 6 Replacement Parts and Repair Items Table 4 Replacement parts and repair items Part No Part Name Description EF2052 End fitting 4 6 mm 2 pieces EF2160 Piping kit 2pes en nuts 1 6 and ferrules 1 16 2pieces EF2161 Ferrule 1 16 Ferrules 1 16 10pieces 7 Troubleshooting Problems in high performance liquid chromatography are attributable to various causes that cannot all be enumerated The table below describes some comparatively common problems related to the column Symptom Cause Measures 1 Column pressure Blocking with foreign matter e Sonicate the filter or replace it rise 1 Dust or insoluble matter in the e Filtrate the mobile phase and mobile phase or sample solution sample solution in advance using 2 Dirt in the tubing a membrane filter 3 Plunger seal fragment e Attach a line filter 4 Precipitation of sample e Clean the tubing and replace the components plunger seal e Prepare a sample solution with the mobile phase 2 Peak splitting 1 Dead volume due to e Reconne
3. ct the tubing http hplc shiseido co jp e column html ce_manual htm 13 02 2014 Ceramospher Chiral Page 4 of 4 tailing and inappropriate connections e Check the column performance broadening 2 Column deterioration using standard samples Not repairable in the case of column deterioration or damage to interior channel 3 Retention time too 1 Liquid leak Indicated on the e Check the pump and tubing for long or unstable pressure gage of the pump any leaks 2 Mixing of air See 2 3 e Secure adequate equilibration time e Purge air RU 2 e Use a degasser RU 2 Ceramospher Chiral RU 1 RU 2 is shipped after a strict performance check However if you should find any defect please contact your dealer or Shiseido for replacement Note that Shiseido does not warrant the product against column life or deterioration caused by the failure to follow the above handling instructions Ten or more days after reception by the customer Shiseido will assume that the product was delivered in good condition and will not accept a later replacement request PAG E i P Our Chromatography Business Contact Shiseido Site Map MZ Analysentechnik GmbH _ I Wohlerstralse 2 6 D 55120 Mainz Tel 49 6131 68 66 19 ANALYSENTECHNIK Fax 49 6131 68 66 20 e mail info mz at de www mz at de http hplc shiseido co jp e column html ce_manual htm 13 02 2014
4. flowchart t Flowchart High loadability 5 to 10 times higher than that of conventional columns for optical separation t Datal Excellent durability t Data Excellent pressure resistance 20 MPa Stable in a wide RU 1 20 to 60 C methanol type temperature range RU 2 15 to 60 C water methanol type Also available outside Japan Property values Type Particle size um Specific surface Acceptable pH Pore size nm area m g RU 1 5 300 3 6 5 4 RU 2 5 300 3 6 5 4 fg re Our Chromatography Business Contact Shiseido site Map http hplc shiseido co jp e column html ce_ index htm 17 02 2014 Ceramospher Chiral Page 1 of 4 SHLSEIDO Ceramospher Chiral User s Manual List of Sales _ Technical Materials _ Representatives Overview User s Manual i Contact Shiseido Catalog List Ceramospher Chiral User s Manual 1 Handling the Column 2 Attaching the Column 3 Analysis 4 Storing the Column 5 End Fittings 6 Replacement Parts and Repair Items 7 Troubleshooting lo Lu la Le Ld a Ceramospher Chiral RU 1 and RU 2 are unique columns for chiral separation that are based on spherical ceramics 1 Handling the Column 1 Handle the column with great care A strong shock may cause damage 2 Attach or detach the column when the pressure gage indicates zero 2 Attaching the Column 1 The column joint is of the male nut type for tubing of 1 16 inch OD Check that the piping joints of
5. from 20 to 50 C if the mobile phase is of the methanol type 2 RU 2 Ceramospher RU 2 is extremely temperature stable Although the operating temperature differs depending on the viscosity and boiling point of the mobile phase Ceramospher RU 2 can be used in the range from 15 to 60 C if the mobile phase is of the water or methanol type a PAGE TOP 4 Storing the Column http hplc shiseido co jp e column html ce_manual htm 13 02 2014 Ceramospher Chiral Page 3 of 4 1 Seal the column with the accessory plug and store it in a cold place where there is little temperature fluctuation 2 After using an aqueous mobile phase or the above mobile phase with acetic acid or amines added rinse the column with methanol and store it after sealing with methanol 5 End Fittings 1 An analytical column of up to 6 mm ID uses a filter embedded end fitting as shown in Fig The filter cannot be changed alone If the filter is clogged or the column pressure is high replace the end fitting See Table 4 for the replacement parts and repair items 2 See Fig 1 for the column connection If the tubing is inappropriate especially if a tube for a different type of column is used the length after the ferrule tip V in Fig 1 is often different from the end fitting length L and a problem may occur If L is greater than V dead volume may be generated and cause peak broadening or tailing or deterioration of separation performance If L is smaller th
6. n column inlet pressure High column pressure due to the use of a high viscosity mobile phase 7 The maximum operating pressure of this column is 20 MPa To ensure that this pressure is not exceeded set the flow rate as follows Set the flow rate and pressure using the following values as the standards reference values 50 C methanol and MPa 0 5mL min 1 0mL min 1 5mL mina 2 0mL min http hplc shiseido co jp e column html ce_manual htm 13 02 2014 Ceramospher Chiral Page 2 of 4 4 6mmx 150mm 3 6 9 9 4 6mmx250mm 5 10 15 25 50 C methanol and MPa 2 RU 2 1 Preparing a Mobile Phase Non Aqueous Type Aqueous Type Water Content 10 70vol Newual Potassium dihydrogenphosphate of C MeOH or acetonitrile 0 5 to 5 mmol L No precipitation ompound allowed Basic 0 1 lvol diethylamine MeOH or 0 01 0 1vol diethylamine MeOH or Compound acetonitrile acetonitrile Acetic acid of 0 01 0 11vol MeOH or acetonitrile Acidic 0 1 lvol acetic acid MeOH or Compound acetonitrile potassium dihydrogenphosphate of 0 5 to 5 mmol L No precipitation allowed Amphoteric a C Derivatize chemically and treat as the neutral above ompound When an acetic acid solution is used the waste liquid is slightly colored However this will not affect column performance Dispose of the waste liquid as heavy metal containing liquid 2 Methanol is sealed in the column at the time of shipment 3 To prevent column deterioration avoid
7. the following Frequent change of the mobile phase composition or direct change to a mobile phase of low compatibility Rapid change in column inlet pressure High column pressure due to the use of a high viscosity mobile phase 4 The maximum operating pressure of this column is 20 MPa To ensure that this pressure is not exceeded set the flow rate as follows Methanol 1 0 mL min Water Methanol 0 5mL min Acetonitrile 1 0mL min Water Acetonitrile 0 5mL min 5 Since potassium dihydrogenphosphate does not easily dissolve in a solution of water methanol or water acetonitrile prepare the solution by the following procedure 1 Dissolve potassium dihydrogenphosphate completely in water 2 Add methanol or acetonitrile to the solution while stirring 3 After degassing check that potassium dihydrogenphosphate is not precipitated 3 2 Preparing a Sample Solution 1 Dissolve the sample in a solvent of the same composition as the mobile phase wherever possible 2 Using a solvent with strong dissolving power may lower separation efficiency or cause the sample to precipitate at the column head 3 If there is insoluble matter remaining in the sample solution filtrate the solution using a filter 0 45 um or smaller 3 3 Temperature 1 RU 1 Ceramospher RU 1 is extremely temperature stable Although the operating temperature differs depending on the viscosity and boiling point of the mobile phase Ceramospher RU 1 can be used in the range
8. the system fit correctly and that the ferrule tips are deeply inserted into the joints See Fig 1 2 Before attaching the column replace the liquid in the system with the mobile phase to be used Refer to the section on mobile phases 3 Attach the column according to the direction of the arrow PAGE TOP 3 Analysis 3 1 Mobile Phase 1 RU 1 1 In addition to methanol non aqueous solvents such as ethanol isopropanol acetonitrile chloroform and n hexane are acceptable It is advised to try methanol at first and to use other solvents only when methanol does not provide a good separation 2 A mobile phase prepared by adding isopropylamine diethylamine triethylamine or acetic acid of up to 1vol to methanol is also acceptable 3 After degassing completely filtrate the mobile phase using a membrane filter 0 45 ui or smaller to remove dust A 2 u filter is used at the column inlet To prevent foreign matter from clogging the column inlet filter we recommend using a line filter 4 Methanol is sealed in the column 5 When using a mobile phase from the same resevoir over a long period it is necessary to take chemical or physical measures to remove moisture out of the liquid 6 To prevent column deterioration avoid the following Injection of a mobile phase or a sample solution containing water Frequent change of the mobile phase composition or direct change to a mobile phase of low compatibility Rapid change i
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