Leica StereoZoom
Contents
1. SZ Leica S4E S6 E S6 T 8 Oi By _ A Dimensions in mm E No 44 058 NE 076 e 1 A 1 ry s O 8 107 42 Leica StereoZoom User Manual Dimensions Leica S6 with inclining focusing drive EV 390 120 46 Leica S6 Dimensions in mm Dimensions Leica S6 D with incident light stand Leica S6 D 058 N s 8 976 Es Ed Ni 107 2 278 393 44 104 Dimensions inmm Leica StereoZoom User Manual 43 Dimensions Leica S6 D Leica S6 D IE O bel g Dimensions in mm 076 o with incident light stand 351 65 22 44 Leica StereoZoom User Manual Dimensions Leica 588 APO with incident light stand Leica S8 APO Dimensions in mm Leica StereoZoom User Manual 45 46 Dimensions Leica S8 APO with transmitted light stand Leica S8 APO 171 je mp 134 Dimensions in mm Leica StereoZoom User Manual Leica Microsystems worldwide Made Leica Leica for you www stereozoom com Leica Microsystems is active in microscopy sample preparation image analysis confocal laser t2. Leica StereoZoom User Manual Leica MICROSYSTEMS Contents Page Information Safety rules 2 0 0 cece cece eee een ees 4 Symbols 12m tas ratem iere 7 Description Features lens system 8 Models cocinan eed A a al 10 Design System diagram 12 Incident light equipment 14 Transmitted light equipment 14 16 qe 16 High performance lighting 17 Leica L2 cold light source 17 Graticules erts 18 Camera assembly for Leica S6 D and S8 APO 20 Operating Controls z pia Ee mre iia 22 Interpupillary distance and exit pupil 23 EVECUDS S 23 Setting working distance and focusing 24 Zoom magnification changer 25 Diopter adjustment 28 Photography with Leica S6 D and S8 APO 32 Special notes Whatdoldoif qta a onp 33 and maintenance 34 Calculating total magnification and field diameter 37 Opticaldata ertet mot 38 Dimension 41 Leica Microsystems 47 Leica StereoZoom User Manual Dear
3. Leica StereoZoom User Manual 15 Assembly Optics carrier gt p Insert optics carrier Stand carefully in the microscope carrier P Fix optics carrier in the desired position with the clamping screw Additional objective Screw selected objective tight optional gt in counterclockwise direction Optics carrier Lens shield gt Screw the lens shield tightly optional into the thread on the Stereo Zoom or onto the additional objective Eyepieces gt p Push eyepiece into the eye Eyepiece tube piece tube as far as it will go gt Check that it sits firmly and snugly The following eyepieces are available for StereoZoom 10x 23 16x 16 20x 12 fixed and adjustable with 12mm exit pubil 10x 23B eyepiece fixed and adjustable for spectacle wearers with 22mm exit pupil 16x 14B 25x 9 5B 40x 6B wide field eyepieces adjustable with 22mm exit pupil Spacing ring required for spectacle wearers 16 Leica StereoZoom User Manual Leica 12 cold light source gt Stand i High performance lighting You can combine your StereoZoom with a fixed and an adjustable eyepiece Two adjustable eyepieces are re quired for equipment which has a reticle in an eyepiece for measurement or photography purposes We recom mend that you also equip the high performance Leica StereoZoom S8 APO with two adjustable eyepieces With its fibre optic light guides the Leica L2 cold light source i
4. e against improper handling Never fit other equipment connectors or dismantle optical systems and mechanical parts unless the user manual gives express instructions for doing so e against oil and grease Never grease guides and mechanical parts Cover your microscope with its dust cover during breaks e Put dust covers over tube openings eyepieces and eyepiece tubes without eyepieces e Remove dust with a rubber blower and soft brush lean eyepieces and objectives with special optical cleaning cloths and pure alcohol e Keep accessories in a dust free environment when not in use Leica StereoZoom User Manual Cleaning plastic parts Never clean plastics To clean plastics safely use A Various components are made of plastic or are plastic coated to make them pleasant to touch and handle However incorrect cleaning with unsuitable detergents can damage the plastic Please observe the following do s and don ts inan ultrasonic cleaner The plastic may become brittle and eventually break with caustic or acetone containing substances such as ether substitute with any other solvents except ethanol and isopropanol warm soapy water rinsing the plastic afterwards with distilled water ethanol industrial alcohol and isopropanol Always take appropriate safety precautions when using ethanol and isopropanol Leica StereoZoom User Manual Care 35 Special notes We guarantee You
5. 40 0 5 6 78 28 7 5 6 40 0 15 0 14 9 11 2 20 0 19 8 113 142 15 8 51 2 44 10 0 224 72 31 1 19 2 11 7 14 3 15 7 25 3 8 9 18 2 12 3 64 0 3 5 12 5 17 9 9 0 24 9 24 0 9 3 17 9 12 5 31 7 7 227 9 9 80 0 28 15 6 144 112 20 0 30 0 15 22 4 10 0 39 6 5 1 28 4 7 9 102 4 22 20 0 11 2 14 3 15 7 38 4 5 8 28 7 78 50 7 44 36 4 6 2 128 0 18 25 0 9 0 17 9 12 5 48 0 4 7 35 8 6 3 63 4 35 454 4 9 31 5 75 6 1 38 9 44 54 0 118 20 1 8 8 21 0 15 6 15 2 11 2 212 40 0 5 9 78 30 4 5 6 424 15 0 15 8 112 212 19 8 12 0 142 16 7 50 0 48 9 8 24 2 70 33 9 18 8 12 6 14 0 17 0 248 9 6 178 13 3 62 5 3 8 12 2 19 5 8 8 27 0 23 4 10 1 17 5 13 6 30 9 77 22 2 10 7 80 0 3 0 15 6 15 2 11 2 21 2 30 0 7 9 224 10 6 39 6 6 0 28 4 84 100 0 24 19 5 122 140 17 0 37 5 6 3 28 0 85 49 5 48 35 5 6 7 125 0 1 9 244 9 7 17 5 13 6 46 9 5 1 35 0 6 8 61 9 3 8 44 4 5 3 160 0 15 312 76 22 4 10 6 60 0 4 0 44 8 5 3 79 2 3 0 56 8 42 200 0 12 39 0 6 1 28 0 8 5 75 0 3 2 56 0 42 99 0 24 710 3 3 50 4 4 8 9 8 24 5 7 33 8 18 9 127 141 17 0 24 9 9 6 17 9 13 4 64 0 3 8 12 5 19 2 9 0 26 7 24 0 10 0 17 9 13 4 31 7 7 6 227 10 6 80 0 3 0 15 6 15 4 11 2 21 4 30 0 8 0 22 4 10 7 39 6 6 1 28 4 8 5 100 0 24 19 5 123 140 17 1 37 5 6 4 28 0 8 6 49 5 4 8 35 5 6 8 128 0 1 9 25 0 9 6 17 9 13 4 48 0 5 0 35 8 6 7 63 4 3 8 45 4 5 3 160 0 15 312 11 22 4 10 7 60 0 4 0 44 8 5 4 79 2 3 0 56 8 4 2 200 0 12 39 0 6 2 28 0 8 6 75 0 3 2 56 0 43 99 0 24 710 3 4 256 0 0 9 49 9 4 8 35 8 6 7 96 0 25 717 3 3 126 7 1 9 90 9 26 320 0 0 8 624 3 8 448 54 1
6. 2 E E E E El E ERE E E 5 E 5 E E 3 3 3 2 3 Eyepieces NE E E e E 2 2 E i 10 446 332 0 63 6 3 36 5 2 0 115 0 3 2 71 9 4 0 57 5 41 48 9 10 1 228 10 446 333 0 8 8 0 28 8 26 88 5 4 0 57 5 5 0 46 0 6 0 38 3 12 8 18 0 1 0 10 0 23 0 3 2 711 9 5 0 46 0 6 3 36 5 75 30 7 16 0 14 4 125 125 18 4 4 0 57 5 6 3 36 5 19 29 1 94 24 5 20 0 11 5 10x 23 16 160 144 51 451 80 288 101 228 120 192 256 90 10x 23B 2 0 20 0 11 5 6 4 35 9 10 0 23 0 12 6 18 3 15 0 15 3 32 0 72 25 25 0 9 2 8 0 28 8 12 5 18 4 15 8 14 6 18 8 12 2 40 0 5 8 10 446 326 32 32 0 72 10 2 22 5 16 0 144 20 2 114 24 0 9 6 51 2 45 10 446 329 4 0 40 0 5 8 12 8 18 0 20 0 11 5 25 2 9 1 30 0 77 64 0 3 6 0 63 10 1 25 3 3 2 80 0 5 0 51 2 6 4 40 0 7 6 33 7 16 1 15 9 0 8 12 8 20 0 41 624 6 4 40 0 8 1 31 6 9 6 26 7 20 5 12 5 1 0 16 0 16 0 5 1 50 2 8 0 32 0 10 1 25 3 12 0 21 3 25 6 10 0 125 20 0 12 8 64 40 0 10 0 25 6 12 6 20 3 15 0 17 1 32 0 8 0 16x 16 16 25 6 10 0 8 2 31 2 12 8 20 0 16 1 15 9 19 2 13 3 41 0 6 2 2 0 32 0 8 0 10 2 25 1 16 0 16 0 20 2 12 7 24 0 10 7 51 2 5 0 2 5 40 0 6 4 12 8 20 0 20 0 12 8 25 2 10 2 30 0 8 5 64 0 40 10 446 354 32 512 5 0 16 4 15 6 25 6 10 0 323 79 38 4 6 7 81 9 3 1 10 446 355 4 0 64 0 4 0 20 5 12 5 32 0 8 0 40 3 6 4 48 0 5 3 102 4 25 0 63 12 6 19 0 4 0 60 0 6 3 38 1 7 9 30 4 9 5 25 3 20 2 11 9 0 8 16 0 15 0 5 1 47 1 8 0 30 0 10 1 23 8 12 0 20 0 25 6 9 4 1 0 20 0 12 0 6 4 37 5 10 0 24 0 12 6 19 0 15 0 16 0 32 0 7
7. 324 shield Adjustable Lens gt 0 3x 0 4x 10446325 10 446 345 10 446 344 10 446 343 10 446 342 Ergolens a 0 6 0 75 10 446 323 S 07 10 10446317 Objetive for S8 APO quim 10 376 070 APO 2 0x 10 446 337 m 0 32x 10 446 334 13312611 APO 0 63x 10 446 335 APO 1 6x 10 446 336 13312 612 13312 613 13312614 13312 610 13 312 714 10 446 299 Leica StereoZoom User Manual 13 Design Basic equipment The components 1 Incident light base with stage plate 2 Transmitted light base with glass stage plate 3 Focus column with microscope carrier 4 StereoZoom optics carrier 5 Optional additional objective 6 Eyepieces fixed and or adjustable Leica StereoZoom User Manual Assembly Never loosen the 3 screws on the right hand side of the focus column Focus column gt Remove stage plate Incident light base gt 3 hexagon head J screws through the under side of the baseplate and screw tight in the focus column gt Replace stage plate Transmitted light base gt Remove glass stage plate Incident lightbase Undo locking device gt Place incident light stand on the transmitted light base and engage in the connecting screw P Close locking device gt Replace glass stage plate gt Insert universal light guide in the socket at the back see user manual for the Leica L2 cold light source C
8. 5 1 25 25 0 9 6 8 0 30 0 12 5 192 15 8 152 18 8 12 8 40 0 6 0 20x 12 1 6 32 0 15 10 2 23 5 16 0 15 0 20 2 11 9 240 10 0 51 2 41 2 0 40 0 6 0 12 8 18 8 20 0 12 0 25 2 9 5 30 0 8 0 64 0 3 8 2 5 50 0 48 16 0 15 0 25 0 9 6 31 5 7 6 37 5 6 4 80 0 3 0 10 446 356 3 2 64 0 3 8 20 5 11 7 32 0 7 5 40 3 6 0 48 0 5 0 102 4 23 10 446 357 4 0 80 0 3 0 25 6 9 4 40 0 6 0 50 4 48 60 0 40 128 0 1 9 0 63 10 1 222 32 70 0 5 0 44 8 6 4 35 0 76 29 5 16 1 13 9 0 8 12 8 17 5 41 54 6 6 4 35 0 8 1 277 9 6 23 3 20 5 10 9 1 0 16 0 14 0 5 1 43 9 8 0 28 0 10 1 222 12 0 18 7 25 6 8 8 1 25 20 0 11 2 6 4 35 0 10 0 22 4 12 6 17 8 15 0 14 9 32 0 7 0 16x 14B 1 6 25 6 8 8 8 2 27 3 12 8 17 5 16 1 13 9 19 2 11 7 41 0 5 5 2 0 32 0 7 0 10 2 22 0 16 0 14 0 20 2 11 1 24 0 9 3 51 2 44 25 40 0 5 6 12 8 17 5 20 0 11 2 25 2 8 9 30 0 75 64 0 35 32 512 44 16 4 13 7 25 6 8 8 32 3 6 9 38 4 5 8 81 9 27 10 445 301 4 0 64 0 3 5 20 5 10 9 32 0 7 0 40 3 5 6 48 0 47 102 4 22 0 63 15 8 15 0 5 0 415 19 30 1 9 9 24 0 11 8 20 1 25 2 9 4 0 8 20 0 11 9 6 4 37 1 10 0 23 8 12 6 18 8 15 0 15 8 32 0 74 1 0 25 0 9 5 8 0 29 7 12 5 19 0 15 8 15 0 18 8 12 6 40 0 5 9 1 25 313 7 6 10 0 23 8 15 6 15 2 19 7 12 1 234 10 1 50 0 48 25x 9 5B 1 6 40 0 5 9 12 8 18 6 20 0 11 9 25 2 9 4 30 0 79 64 0 3 7 2 0 50 0 4 8 16 0 14 8 25 0 95 315 75 37 5 6 3 80 0 3 0 25 62 5 3 8 20 0 11 9 31 3 7 6 39 4 6 0 46 9 5 1 100 0 24 32 80 0 3 0 25 6 9 3 40 0 5 9 50 4 47 60 0 40 128 0 1 9 10 445 302 4 0 100 0 24 32 0 74 50 0 48 63 0 3 8 75 0 3 2 16
9. Customer Thank you for the trust you have shown in choosing one of our products We hope you will have much enjoyment and success with your new stereomicroscope from Leica Microsystems With its new StereoZoom line the Swiss Stereomicro scopy Business Unit offers a comprehensive range of stereomicroscopes for every application The Leica L2 cold light illuminator also new transforms any of the instruments into a compact streamlined complete outfit of modern design Possible applications range from in spection and assembly work OEM and schools to quality assurance functions The stereomicroscopes cold light source and stand are all antistatic to protect against ESD A Terminator version is available for problematic working areas in electronics One of our major development goals was to make our new StereoZoom line simple and intuitive to use Never theless please take the time to read these instructions They will familiarize you with your stereomicroscope s advantages and enable you to use it to best effect If you have questions at any time please contact your Leica representative or Leica Microsystems Switzerland Ltd Heerbrugg Switzerland We will be glad to help you We place great emphasis on customer service before and after purchase Leica StereoZoom User Manual Safety rules General instructions Intended application Improper use Place of use Service work Obligations of the operator Re
10. Medical Equipment Netherlands Rijswijk Tel 31 70 41 32 130 Fax 31 70 41 32 109 Innovative technologies in our surgical micro Portugal Lisbon Tel 35 1 213 889 112 Fax 35 1 213 854 668 scopes offer new therapeutic approaches in Singapore Tel 65 6 77 97 823 Fax 465 6 77 30 628 microsurgery With automated instruments for Spain Barcelona Tel 34 93 494 9530 Fax 34 93 494 9532 ophthalmology we enable new diagnostic Sweden Sollentuna Tel 46 8 625 45 45 Fax 46 8 625 45 10 methods to be applied Switzerland Glattbrugg Tel 41 1 809 34 34 Fax 41 1 809 34 44 United Kingdom Milton Keynes Tel 44 1908 666 663 Fax 44 1908 609 992 Semiconductor Equipment USA Bannockburn lllinois Tel 1 800 248 0123 Fax 1 847 405 0164 Our automated leading edge measurement and representatives of Leica Micro in more than 100 countries and inspection systems and our E beam lithography systems make us the first choice ystems supplier for semiconductor manufacturers all over the world The Business Units in Leica Microsystems hold the management system certificates for the international standards ISO 9001 and ISO 14001 relating to quality management quality assurance and environmental management Leica Microsystems Switzerland Ltd Business Unit SM CH 9435 Heerbrugg Telephone 41 71 726 33 33 Fax 41 71 726 33 99 MICROSYSTEMS www leica microsystems com www stereomicroscopy com Illustrations descriptions and techn
11. are working with a high performance precision quality instrument whose quality we guarantee The warranty covers manufacturing and materials faults but not damage resulting from negligence or incorrect handling If you treat your valuable optical instrument with due care it will repay you with the decades of reliable precision for which Leica instruments are renowned However should you encounter problems with your micro scope at any time please contact your local Leica represen tative or Leica Microsystems Ltd Heerbrugg Switzerland 36 Leica StereoZoom User Manual Calculating total Mg Objective magnification magnification and Eyepiece magnification field diameter Magnification changer setting Nfoy Eyepiece field number Field numbers are printed on the eyepieces 10x 23 16x 16 20x 12 10x 23B 16x 14B 25x 9 5B 40x 6B Example Mo 1 6x objective ME 20x 12 eyepiece z Zoom position 4 0 Magnification in the binocular tube vis Mo x Mg xz 16x20x4 128 Field diameter in the object Nrov 12 1 9mm o Mo xz 1 6 x 4 Leica StereoZoom User Manual Special notes 37 Optical Data Leica 54 E S6 E 56 56 T S6 D with additional objectives 0 32x 0 5x 0 63x 0 75x 1 6x Working distance 110mm 300mm 200mm 155mm 130mm 55mm E E 5 E 5 E E E E 5 E 8 5 E u E E E 2 2 2 2 2 2 E 5 E
12. focusing drive 30 Leica StereoZoom User Manual Regulating diopters Testing parfocality e When doing this the sharp Set lowest magnification Do not look into the eyepiece Turn lens counterclockwise in the direction until it stops Now look into the eyepiece Close one eye With the other eye observe the test object and turn the lens slowly clockwise in the direction until this eye sees the object clearly Setthe diopter for the other eye in the same way Select highest magnification View object and if necessary gently refocus Adjust magnification changer from the lowest to the highest magnification ness must remain constant over the entire zoom range parfocal Otherwise please repeat the procedure Leica StereoZoom User Manual Special notes 31 Operating Photography with Itis possible to switch between the observation Leica S6 Dand and photo beam paths Light distribution S8 APO both eyepieces no light in zh the video photo beam path KC in position 100 light vIS in the right eyepiece no light NC in the left eyepiece 10096 o ses light in the video photo beam path gt When the picture detail and sharpness are set to your satisfaction switch to the Doc position and take your photo 32 Leica StereoZoom User Manual Special notes What do do if Remedy Remedy Remedy Remedy Remedy Remedy the f
13. of microstructures segments where we rank with the market leaders Leica the leading brand for microscopes and scientific instruments has developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Leica symbolizes not only tradition but also innovation Microscopy Our expertise in microscopy is the basis for all our solutions for visualization measurement Leica Microsystems an international company and analysis of microstructures in life sciences with a strong network of customer services LEA Australia Gladesville NSW Tel 1 800 625 286 Fax 461 2 9817 8358 Specimen Preparation Austria Vienna Tel 43 1486 80500 Fax 43 1 486 80 50 30 We specialize in supplying complete solutions Canada Richmond Hill Ontario Tel 1 905 762 2000 Fax 1 905 762 89 37 for histology and cytopathology China Hong Kong Tel 48522 564 6699 Fax 48522 564 4163 Denmark Herlev Tel 45 44 5401 01 Fax 45 44 5401 11 Imaging Systems France Rueil Malmaison With confocal laser technology and image C dex Tel 433147328585 Fax 33 1 4732 8586 analysis systems we provide three dimensional Germany Bensheim Tel 49 6251 1360 Fax 49 6251 136 155 viewing facilities and offer new solutions for Italy Milan Tel 39 02 57 486 1 Fax 39 02 5740 3273 cytogenetics pathology and material sciences Japan Tokyo Tel 4813543 59609 Fax 813 543 596 15 Korea Seoul Tel 82 2 514 6543 Fax 482 2 514 6548
14. 0 0 1 5 0 63 25 2 9 5 8 1 29 6 12 6 19 0 15 9 15 1 18 9 12 7 40 3 6 0 0 8 32 0 75 10 2 23 5 16 0 15 0 20 2 11 9 24 0 10 0 51 2 47 1 0 40 0 6 0 12 8 18 8 20 0 12 0 25 2 9 5 30 0 8 0 64 0 3 8 1 25 50 0 48 16 0 15 0 25 0 9 6 31 5 76 37 5 6 4 80 0 3 0 40x 6B 1 6 64 0 3 8 205 117 32 0 75 403 6 0 480 50 1024 23 2 0 80 0 3 0 25 6 9 4 40 0 6 0 50 4 4 8 60 0 4 0 128 0 1 9 25 100 0 24 32 0 75 50 0 48 63 0 3 8 75 0 3 2 160 0 1 5 321 128 0 1 9 41 0 5 9 64 0 3 8 80 6 3 0 96 0 2 5 204 8 12 10 445 303 4 0 160 0 1 5 51 2 47 80 0 3 0 100 8 24 120 0 2 0 256 0 0 9 38 Leica StereoZoom User Manual with additional objectives 2 0x 0 3x 0 4x 0 3x 0 4x 0 6 0 75 0 6x 0 75x 0 7x 1 0x 0 7x 1 0x 35mm 200mm 350mm 71mm 137mm 48mm 98mm S s S 5 S 5 S 5 S 5 S 5 S 5 2 2 g 2 2 2 2 E e E 2 E i 2 5 E 5 5 E 5 p 2 2 2 E LS e 126 18 3 25 92 0 18 127 8 41 48 9 3 5 65 7 6 2 37 1 45 51 1 16 0 14 4 3 1 74 2 2 2 104 5 6 0 38 3 45 51 1 79 29 1 5 7 40 4 20 0 11 5 3 9 59 0 2 8 82 1 75 30 7 5 6 41 1 9 9 23 2 71 32 4 25 0 9 2 49 46 9 3 5 65 7 9 4 24 5 7 0 32 9 12 4 18 5 8 9 25 8 32 0 72 6 2 371 45 51 1 12 0 19 2 9 0 25 6 15 8 14 6 11 4 20 2 40 0 5 8 78 29 5 5 6 41 1 15 0 15 3 11 2 20 5 19 8 11 6 14 2 16 2 50 0 46 9 8 23 5 7 0 32 9 18 8 12 2 14 0 16 4 24
15. 2 Objectives 3 Eyepieces 4 Carriers Focus arms Detailed Descriptions see Brochure M1 188 4en e Low Eyepoint Eyepieces Eyepieces fixed Eyepieces adjustable 10x 23 10446332 10x 23 10 446 333 5 Focus columns 19x16 10446354 KO 16 16 10446 355 20x 12 10446 356 20x 12 10446 357 6 Incident light bases 7 Transmitted light Adjustable eyepieces accept reticles see price list bases 8 Stages 9 Stands Swing arm Flex arm Leica S4 E Leica S6 E E Leica CA 446 293 10 446 294 10 446 295 10315306 gt 10 445 616 10 446 341 10 446 348 10 446 394 10 445 387 10 445 367 10 445 363 c E 10 447 057 10 447 058 10 446 301 10 446 303 10 447 159 Leica StereoZoom User Manual High Eyepoint Eyepieces All High Eyepoint Eyepieces include Eyeguards Eyepiece fixed Eyepieces adjustable E Adjustable eyepieces accept reticles 10x 23 10446326 10 23 10446 329 see price list 16x 14B 10 445 301 e 10447 036 25x 9 5B 10 445 302 Spacing Ring required with eyepiece 40x 6B 10445303 10 445 301 10 445 302 10 445 303 Photo Video documentation Leica DC cameras 5 Leica S6 T Leica S6 D Leica S8 APO o 10 446 296 10 446 297 J 10446 298 Objectives for S4 E S6 E S6 S6 T 0 32x 10 446 316 0 5 10 446 318 0 63x 10 446 319 0 75x 10 446 320 1 6x 10 446 321 2 0x 10 446 322 amp Lens 10 446
16. 20 0 20 89 6 27 158 4 1 5 113 6 21 Leica StereoZoom User Manual Optical Data StereoZoom S8 APO Apochromats Achromate 0 63x 1 6x 2 0x 0 32x Working distance 75mm 101mm 37mm 25mm 200mm E E E E E E E E E E E El E 5 5 5 8 Eyepieces e 2 e 2 e 2 e 2 e 2 10 446 332 1 0 10 0 23 0 6 3 36 5 16 0 144 20 0 11 5 32 71 9 10 446 333 1 25 12 5 18 4 7 9 29 1 20 0 11 5 25 0 92 40 57 5 1 6 16 0 144 10 1 228 25 6 9 0 32 0 7 2 51 45 1 2 0 20 0 11 5 12 6 18 3 32 0 72 40 0 5 8 64 35 9 10 23 25 25 0 92 158 14 6 40 0 58 50 0 46 8 0 288 10x 23B 32 32 0 12 20 2 11 4 51 2 45 64 0 3 6 10 2 225 40 40 0 5 8 25 2 9 1 64 0 3 6 80 0 2 9 12 8 18 0 5 0 50 0 4 6 31 5 7 3 80 0 2 9 100 0 2 3 16 0 14 4 10 446 326 6 3 63 0 37 39 7 5 8 100 8 23 126 0 18 20 2 114 10 446 329 8 0 80 0 29 50 4 46 128 0 18 160 0 14 25 6 9 0 1 0 16 0 16 0 10 1 25 3 25 6 10 0 32 0 8 0 5 1 43 9 1 25 20 0 12 8 12 6 20 3 32 0 8 0 40 0 6 4 6 4 35 0 1 6 25 6 10 0 16 1 15 9 41 0 6 2 51 2 5 0 8 2 27 3 2 0 32 0 8 0 20 2 12 7 51 2 5 0 64 0 4 0 10 2 22 0 25 40 0 6 4 25 2 10 2 64 0 4 0 80 0 3 2 12 8 17 5 16 16 3 2 51 2 5 0 32 3 7 9 81 9 3 1 102 4 25 16 4 13 7 4 0 64 0 4 0 40 3 6 4 102 4 25 128 0 2 0 20 5 10 9 5 0 80 0 3 2 50 4 5 1 128 0 2 0 160 0 1 6 25 6 8 8 10 446 354 6 3 100 8 25 63 5 4 0 161 3 16 201 6 1 3 32 3 6 9 10 446 355 8 0 128 0 2 0 80 6 3 2 204
17. 7 Camera assembly for Leica S6 D and S8 APO Format reticle Leica StereoZoom S6 D and S8 APO are equipped with a built in video photo tube that allows for simple quick set ting up of digital film and analog video cameras Please ask your Leica advisor about the options You can find detailed information about Leica camera systems accessories and image management software in the corresponding manuals gt Remove the dust cover from the video photo output gt Always close the video photo output with the dust cover if there is no camera attached gt Attach appropriate video or photo objective accor ding to which camera is used see scheme p 20 The format reticle is used to test the sharpness and picture detail Borderlines are displayed on the reticle for all film formats The left observation beam path and the photo output are aligned with one another Always judge therefore the picture detail and the sharpness in the left eyepiece gt Insert format reticle into an adjustable eyepiece see p 18 p Insert eyepiece with reticle into the left tube Leica StereoZoom User Manual Assembly 21 Operating Overview Standard controls and their functions 22 Co N a C co co Magnification changer right hand pinion knob with magnification scale S6 models zoom limiter stop Focusing drive Fixing screw fixes the optics carrier in the microscope carrier Adjustabl
18. 8 13 256 0 1 0 41 0 5 5 10 25 0 9 5 15 8 15 0 40 0 5 9 50 0 4 8 8 0 297 1 25 31 3 7 6 19 7 12 1 50 0 48 62 5 3 8 10 0 23 8 1 6 40 0 5 9 25 2 9 4 64 0 3 1 80 0 3 0 12 8 18 6 20 50 0 48 31 5 15 80 0 3 0 100 0 24 16 0 148 25 62 5 3 8 39 4 6 0 100 0 24 125 0 1 9 20 0 11 9 25x 9 5B 32 80 0 3 0 50 4 4 7 128 0 1 9 160 0 15 25 6 9 3 4 0 100 0 24 63 0 3 8 160 0 15 200 0 12 32 0 74 5 0 125 0 1 9 78 8 3 0 200 0 12 250 0 10 40 0 5 9 6 3 157 5 15 99 2 24 252 0 0 9 315 0 0 8 504 4 7 10 445 302 8 0 200 0 12 126 0 1 9 320 0 07 400 0 0 6 64 0 37 1 0 40 0 6 0 25 2 9 5 64 0 3 8 80 0 3 0 12 8 18 8 1 25 50 0 4 8 31 5 7 6 80 0 3 0 100 0 24 16 0 15 0 1 6 64 0 3 8 40 3 6 0 102 4 23 128 0 1 9 20 5 117 20 80 0 3 0 504 48 128 0 19 160 0 15 25 6 9 4 25 100 0 24 63 0 3 8 160 0 15 200 0 12 32 0 15 40x 6B 3 2 128 0 1 9 80 6 3 0 204 8 12 256 0 0 9 41 0 5 9 4 0 160 0 15 100 8 24 256 0 0 9 320 0 0 8 51 2 4 7 5 0 200 0 12 126 0 18 320 0 0 8 400 0 0 6 64 0 3 8 6 3 252 0 1 0 158 8 15 403 2 0 6 504 0 0 5 80 6 3 0 10 445 303 8 0 320 0 0 8 201 6 12 512 0 0 5 640 0 0 4 102 4 23 40 Leica StereoZoom User Manual Dimensions Leica S4 E S6 E S6 T with incident light stand 351 Leica S4 E S6 E S6 T Dimensions in mm O e E y t 107 al 278 393 gt Q CT Leica StereoZoom User Manual 4 Dimensions Leica S4 E S6 E S6 T with transmitted light stand
19. 8 9 3 178 12 9 64 0 3 6 12 5 18 4 9 0 25 6 24 0 9 6 17 9 12 8 31 7 73 22 7 10 1 80 0 29 15 6 14 7 11 2 20 5 30 0 11 22 4 10 3 39 6 5 8 28 4 8 1 20 2 12 7 3 9 65 6 28 91 4 76 33 7 5 6 45 7 10 0 25 6 7 2 35 6 25 6 10 0 5 0 51 2 3 6 71 1 9 6 26 7 7 2 35 6 12 7 20 2 9 1 28 1 32 0 8 0 6 2 41 3 45 56 9 12 0 21 3 9 0 28 4 15 8 16 2 11 4 22 5 40 0 6 4 7 8 32 8 5 6 45 7 15 0 17 1 11 2 22 9 19 8 12 9 142 18 0 512 5 0 10 0 25 6 72 35 6 19 2 13 3 143 17 9 25 3 10 1 182 14 1 64 0 4 0 12 5 20 5 9 0 28 4 24 0 10 7 17 9 143 317 8 1 22 1 11 3 80 0 3 2 15 6 16 4 11 2 22 9 30 0 8 5 22 4 11 4 39 6 6 5 28 4 9 0 102 4 25 20 0 12 8 143 17 9 38 4 6 7 28 7 8 9 50 7 5 0 36 4 70 128 0 2 0 25 0 10 2 17 9 143 48 0 53 35 8 7 2 63 4 4 0 454 56 25 2 9 5 49 49 0 35 68 6 9 5 25 3 7 33 8 12 5 19 2 8 9 27 0 32 0 75 6 2 38 7 45 53 3 12 0 20 0 9 0 26 7 15 8 15 2 114 21 1 40 0 6 0 78 30 8 5 6 42 9 15 0 16 0 112 214 19 8 12 1 14 2 16 9 50 0 48 9 8 24 5 7 0 34 3 18 8 12 8 14 0 171 24 8 97 17 8 13 5 64 0 3 8 12 5 19 2 9 0 26 7 24 0 10 0 17 9 13 4 31 7 7 6 227 10 6 80 0 3 0 15 6 15 4 11 2 21 4 30 0 8 0 22 4 10 7 39 6 6 1 28 4 8 5 100 0 24 19 5 123 140 17 1 37 5 6 4 28 0 86 49 5 48 35 5 6 8 128 0 1 9 25 0 9 6 179 134 48 0 5 0 35 8 6 7 63 4 3 8 45 4 5 3 160 0 15 312 77 22 4 10 7 60 0 4 0 44 8 5 4 79 2 3 0 56 8 42 20 2 11 1 3 9 57 4 2 8 80 0 76 29 5 5 6 40 0 10 0 224 72 31 1 25 6 8 8 5 0 448 3 6 622 9 6 23 3 12 31 1 12 7 17 6 9 1 24 6 32 0 7 0 6 2 36 1 45 49 8 12 0 18 7 9 0 24 9 15 8 142 114 19 6
20. ad the instructions for use and safety instructions before putting the stereomicroscope into operation Leica S4 E S6 E S6 S6 T S6 D and S8 APO stereomicro scopes are precision optical instruments for making tech nical and scientific objects object details or specimens more easily visible by means of magnification Stands illuminators and accessories complete the equip ment e Use of the instrument other than in the manner de scribed in these instructions could result in personal injury or material damage e Never fit other equipment connectors or dismantle optical systems and mechanical parts unless the user manual gives express instructions for doing so e The Leica S4 E S6 E S6 S6 T S6 D and S8 APO stereo microscopes are intended primarily for indoor use e fused outdoors the stereomicroscope must be pro tected against dust and moisture Leica Microsystems electric illuminators and stands must not be used outdoors Repair work must only be carried out by service engineers trained by Leica Microsystems Only original spare parts from Leica Microsystems are to be used Make sure that personnel using this stereomicroscope have read and understood these instructions espe cially the safety instructions e Take steps to ensure that Leica S4 E S6 E S6 S6 T S6 D and S8 APO stereomicroscopes are only operated serviced and repaired by authorized and trained personnel Leica StereoZoom User Manual Safety in
21. e eyepiece tubes Interpupillary distance adjustable from 55 75mm Eyepieces Lamp bracket mounting thread on both sides and at rear Socket for connecting for the Leica L2 universal light guide not for Leica S8 APO Thread for additional objective lens shield Leica StereoZoom User Manual Interpupillary The interpupillary distance can be distance and adjusted from 55 75mm exitpupil The exit pupil is the distance between the eye and eyepiece It is 12mm the 10x 23 16x 16 and 20x 12 standard eyepieces 22mm on the 10x 23B fixed and adjustable for spectacle wearers and on the 16x 14B 25x 9 5B and 40x 6B wide field eyepieces for spectacle wearers gt Carefully put your eyes against the eyepieces and push the eyepiece tubes together or apart until with both eyes you can see a single shadow free circular field Eyecups The 10x 23B 16x 14B 25x 9 5B and 40x 6B eyepieces for spec tacle wearers are supplied with detachable eyecups If you do not wear spectacles and want the eyecups to fit snugly round your eyes P Attach the eyecups to the eyepieces You wear spectacles for your work p Fold the eyecups downwards The folded down eyecups will protect your spectacle lenses against scratching Leica StereoZoom User Manual Operating 23 Operating Setting working distance and focusing Tension adjustment 24 To focus the stereomicroscope raise or lower
22. e lamp housing or trans mitted light stand when the instrument is connected to the mains Wait for filament lamps to cool down before changing Touching hot filament lamps can result in burns Observe all statutory accident prevention and environ mental protection requirements Electrically operated accessories for Leica S4 E S6 E S6 S6 T S6 D and S8 APO stereomicroscopes are manufac tured using state of the art technology and carry an EC declaration of conformity Leica StereoZoom User Manual Safety instructions Symbols PP You will come across these symbols in this manual Safety warnings This symbol denotes information that must be read and acted upon without fail Disregarding safety warnings can put persons at risk Disregarding safety warnings can also result in malfunc tioning or damage to the instrument Warning against exposed hot points e g filament lamps Risk of burns if disregarded Important information This symbol denotes additional information or explana tions to assist understanding Action P This symbol denotes action to be taken Additional information This symbol denotes additional information or explanations Leica StereoZoom User Manual Description Greenough optical system Protection against ESD Photography The optical system of the Leica StereoZoom line con sists of two beam paths converging at 12 Since the pairs of objectives are ve
23. echnology medical equipment and equipment for the semiconduc tor industry With headquarters in Wetzlar Germany this international technology group has grown from five companies with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Leica Microsystems develops high utility end to end solutions and innovative cutting edge technologies of internationally renowned quality The same high quality standards are in force in each of the Group s 11 pro duction sites in seven countries making the name Leica synonymous with quality Technology is one key factor in the success of Leica Microsystems customer focus the other Our friendly staff offer competent on the spot advice and service in your own language Naturally we also cater reliably and without fuss for all customer wishes and ensure that your goods arrive on time and in perfect condition You will find valuable information on the products and services of Leica Microsystems on the Internet as well as contact information for distributors near you Visit us to get more information on the new StereoZoom line Leica StereoZoom User Manual 47 Leica Microsystems the brand for outstanding products Leica Microsystems Mission is to be the world s first choice provider of innovative The companies of the Leica Microsystems solutions to our customers needs for vision measurement lithography and analysis Group operate internationally in five business
24. ereoZoom User Manual Diopter adjustment p Without looking into the eye atthe adjustable pieces turn the eyelens of the eyepiece adjustable eyepiece as far as possible in the direction gt Close the eye that is looking into the fixed eyepiece and look into the adjustable eyepiece with the other eye gt View the test object and slowly turn the eyelens clock wise the direction until the object is in focus gt Setthe microscope to maximum magnification gt View the test object with both eyes and bring it into sharp focus with the focusing drive Checking View the object while zooming parfocality from minimum to maximum magni fication The object should remain in constant focus parfocal at all times If it does not please repeat the procedure above Leica StereoZoom User Manual Operating 29 Special notes Setting up diopter with two adjustable eyepieces Preparations gt gt Focusingon p test object For Leica S6 D and S8 APO set pinion knob to Vis position Set approximate working distance by means of focusing drive working distances of various objectives see 38 40 Set up lighting Set viewing distance p 23 Set 0 diopters on both eyepieces Place flat test object under the objective Set lowest magnification View test object through the eyepiece and focus using the focusing drive Set highest magnification Optimize sharpness using the
25. ical data are not binding and may be changed without notice M2 188 0en Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg 2002 VI 2002 RDV
26. ield of vision is in shadow Adjust interpupillary distance and ensure that it is correct p 23 Check pupil position p 23 the image will not stay in focus Insert eyepieces correctly p 16 Correct diopter adjustment exactly as described p 28 31 the focusing drive slides down or is difficult to turn Adjust tension p 24 If you encounter problems with electrically powered equipment first check that the voltage selector is set to the correct voltage that the mains power switch is in the on position that the mains power cable is correctly connected that all connector cables are correctly connected that none of the fuses have blown the image is too dark Set regulating knob high enough Photographs are blurred Focus precisely p 24 Focus on reticles and carry out diopters correction exactly according to the instructions p 30 Insert eyepieces all the way in p 16 Check that the reticles are firmly placed in the eyepiece p 18 No image on the film Switch light divider on the photo tube to the Doc position p 32 Leica StereoZoom User Manual 33 Protect your microscope Dust and dirt impair your results Therefore 34 In this section we would like to explain how you can take care of your microscope and give you a few tips on cleaning against moisture vapours acids alkalis and caustic substances Never keep chemicals close to your microscope
27. it using the focusing drive until the desired object segmentis in focus i e inside the objective s working distance The working distances of the various objectives are listed in the tables on pages 38 40 You can operate the focusing drive with either your right or left hand Position the object under the objective Select the minimum magnification You should select the mini mum magnification because itis easier to find the desired object segment in a large field of vision Look into the eyepieces Focus the object by turning the pinion knob Is the focusing drive too stiff too loose or does the set up slip down unassisted You can tension the focusing drive to suitthe weight of the set up and your own needs Take one pinion knob in each hand and turn in opposing directions until the desired tension is reached Leica StereoZoom User Manual Tilting the optics carrier to a lateral position Zoom magnification changer Changing magnification The optics carrier can be tilted sideways if the user wants to work from a lateral position gt Loosen the clamping bolt P Tilt the optic carrier side ways to the desired position P Tighten the clamping bolt All StereoZoom models are equipped with a continuously adjustable magnification changer that can be operated with either the right or left hand The right hand pinion knob carries a magnification scale The ranges are 0 63
28. oZoom User Manual Operating 27 Operating 28 Diopter adjustment and setting parfocality Preparations Bring the test object into focus If you adjust the diopters at the adjustable eyepiece as described below the focus will remain constant or par focal from minimum to maximum magnification In other words you will not have to refocus when you change magnification but only when you want to examine a higher or lower positioned object segment Make full use of this feature which is not available on every stereo microscope Diopter adjustment is possible within a range from 5 to 5 Every user has to make the following adjustments only once If you are using a graticule the diopter adjustment and parfocality setting procedure is slightly different to that described here Please refer to the section on measure ment in the graticule user manuals Setting up diopter with an adjustable and a fixed eyepiece gt Set up illuminator gt Set interpupillary distance p 23 gt Set approximate working distance using the focusing drive see p 38 40 for the working distances of the various objectives p Position a flat test object beneath the objective gt Set the microscope to minimum magnification Close the eye that is looking into the adjustable eyepiece and look into the fixed eyepiece with the other eye gt View the test object and bring into focus with the focusing drive Leica St
29. pe on which side the vacuum metalized scale is located The scale should be visible on the correct side gt Pull out the insert from the bottom of the eyepiece and place it with the knurled side on the table Leica StereoZoom User Manual gt Pick up the graticules at the edge so as not to leave fingerprints and slide into the holder from the side P Put the insert back into the eyepiece and press firmly in gt place gt Insert the eyepiece into the LJ eyepiece tube and line up the graticule by turning the eyepiece in the eyepiece tube The measuring process is described in the Measuring instructions Leica StereoZoom User Manual Assembly 19 Assembly 1 2 3 1 2 1 3 C Mount CCD cameras Leica DC cameras SLR camera housings 10 445 929 DC350 DC500 11 541 007 0 5x 119445 928 11541 016 1 2 0 5x 2 Leica S6 D wa 10 446 297 20 Leica StereoZoom User Manual Leica 3 Chip 2 3 3 Chip 1 2 1 MPS30 MPS60 B Mount B Mount C Mount Photoautomats CCD camera CCD camera CCD camera 11 543 703 11 543 702 11 543 706 _ e e _ Adapter SLR 11 541 038 10 446 120 CTI 8x e 10 445 304 10 162 226 10x 10 445 305 16 10 446 261 10 445 541 11 541 006 2 3 0 63x i 1 1x DC300 DC150 DC300 F C 10 446 175 10 404 207 10 445 932 T y 10 445 930 1 0x Leica S8 APO 10 446 298 aj
30. ry close together the stereo microscopes can be constructed very slim towards the base This has a number of advantages less space required for use on bonders and machines unimpeded working on the object plenty of room for tools free view of the object field The Greenough system provides a low cost solution for correcting aberrations such as chromatism curvature of the field of view and distortion On the new Leica Stereo Zoom line the optimally corrected centre ofthe objec tive is used for the image This results in excellent optical performance with large flat distortion free fields of vision and high contrast images with maximum chromatic correction The Leica S4 E S6 E S6 S6 D and S8 APO stereomicro scopes including cold light source and stand are made of antistatic material with a surface resistance of 2x10 ohm square and a discharge time from 1000V to 100V of under 2 seconds The Leica S6 T Terminator for problematical work areas and the T incident light stand are made of antistatic ma terial with a surface resistance of 107 105 ohm square and a discharge time from 1000V to zero of under 0 1 seconds The Leica StereoZoom S6 D and S8 APO models are equipped with a built in video photo tube that allows for simple quick setting up of digital film and analog video cameras Leica StereoZoom User Manual World first Leica StereoZoom S8 APO is the first stereomicroscope on the market wi
31. s the illumination of choice for the Leica S4 E S6 E S6 and S6 T stereomicroscopes Mat ching adapters are available for connecting the Leica L2 cold light source to various stereomicroscope stands and for standalone operation Detailed information on the construction and use of the Leica L2 can be found in the relevant user manual Please note that the universal light guide on the Leica S8 APO can only be used with the sideways mounted lamp arm For higher requirements e g for photography or in combination with the Leica S8 APO we offer a diverse high performance transmitted light stand and low voltage lighting Please ask your Leica advisor about the options Leica StereoZoom User Manual Assembly 17 Assembly Graticule gt Adjustable eyepieces Jj o The following graticules and stage micrometers are available for calibration Graticule 10mm 0 1mm Graticule 5mm 0 1mm Graticule 5mm 0 05mm Graticule 100 div 0 002 Graticule 100 div 0 001 Graticule 150 div 0 0005 Crosshair Stage micrometer 50mm 0 1 0 01mm divisions Stage micrometer 1 0 001 divisions For photography a 10x format reticle is available Two adjustable eyepieces are required for equipment which has a reticle in an eyepiece for measurement or photography purposes The graticules can be inserted into adjustable eyepieces and eyepieces for spectacle wearers gt Ascertain with the aid of the stereomicrosco
32. structions Liquids Mains cable Opening the instrument Mains voltage Electrically operated equipment Handle liquids carefully Liquids spilt on the instrument cause the stereomicroscope and other equip mentto become electrically live and injure persons cause damage to the instrument Check regularly to ensure that the mains cable is undamaged and avoid jerking or pulling the cable hard Faulty mains cables caninjure persons cancause the stereomicroscope and other equip ment to become electrically live and injure persons Ensure the cable is not in a position where someone could accidentally catch it otherwise the instrument could tip over and fall damaging itself or other equip ment or injuring people nearby Electrical equipment may only be repaired by authorized Leica personnel Disconnectthe mains cable before opening the instru ment Touching the open instrument when voltage is applied may result in injury Make sure that the instrument is set for the correct mains voltage Incorrect setting can damage the instru ment Leica StereoZoom User Manual Connections Changing lamps Statutory regulations EC declaration of conformity Only devices with the correct power rating may be connected to the transformers Overloading can result in damage to the instrument Disconnectthe mains cable from the mains supply before changing lamps e Never put your hand in th
33. th completely apochromatic corrected Greenough system Apochromatic optics corrects per fectly chromatic aberrations eliminates interfering color fringes and renders even the finest details ultra sharp Contrast brilliance sharpness resolution color fidelity and reproduction accuracy are unsurpassed The advan tage of apochromatic correction is best observed in objects that have fine low contrast structures such as large animal cells cilia plants or metallic microelectronic structures Features StereoZoom Zoom Magnification Viewing Extras angle Leica S4 E 4 8 1 6 3x 30x 38 ErgoLens Leica S6 E 6 3 1 6 3x 40x 38 ErgoLens Leica S6 6 3 1 6 3x 40x 60 ErgoLens Leica S6 T 6 3 1 6 3x 40x 38 Terminator ErgoLens Leica S6 D 6 3 1 6 3x 40x 38 Video photo tubes Ergono mic objectives Leica S8 APO 8 1 10x 80x 38 Apochromatic Greenough system Apochromatic zoom Apochromatic objectives Video photo tube StereoZoom is registered as a trademark in the Principal Register of the US Patent and Trademark Office Leica StereoZoom User Manual Description 9 StereoZoom Leica S4 E StereoZoom Leica S6 E StereoZoom Leica S6 Leica Design by Christophe Apoth loz 10 Leica StereoZoom User Manual Description StereoZoom Leica S6 T StereoZoom Leica S6 D StereoZoom Leica S8 APO Leica StereoZoom User Manual Description Design 1 StereoZoom Op tics carriers
34. to 3 on the S4 E and 0 63 to 4 on the S6 models for S8 APO from 1 to 8 The tables on pages 38 to 40 list the magnifications and field diameters as a function of magnification changer position and the eyepiece objective combination being used gt Look into the eyepieces gt Bring the object into focus p 24 p Rotate the magnification changer until the desired magnification is achieved Leica StereoZoom User Manual Operating 25 Operating Setting the On the S6 models and for the S8 APO it is zoom limiter possible to fix the maximum and minimum zoom The same function can be used to set a fixed level of magnification Example 1 Set the minimum zoom level at 1 using the Setting azoom stop on the left hand pinion knob range of 1 to 3 2 p Loosen the hexagon head screws on the left hand pinion knob using the Allen key supplied gt Turn the right hand pinion knob to 1 gt Move the stop on the left hand pinion knob forwards until ittouches the built in zoom stop P Tighten the hexagon head screws 26 Leica StereoZoom User Manual 2 Set the maximum zoom level at 3 2 using the stop on the right hand pinion knob p Loosen the hexagon head screws on the right hand pinion knob gt Turn the right hand pinion knob to 3 2 gt Move the stop on the right hand pinion knob backwards until it touches the built in zoom stop P Tighten the hexagon head screws Leica Stere
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