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1. Care and Use Instructions YMC Semi Preparative Guard Cartridge 1 Introduction YMC Semi Preparative Guard Cartridge is a guard cartridge column packed under high pressure with packing materials of various functional groups 2 Structure of YMC Semi Preparative Guard Cartridge YMC Semi Preparative Guard Cartridge is composed of a pair of cartridge Guard cartndge column holders and a cartridge column J The cartridge holders are reusable In case of degradation replace the cartridge column only YMC Sem Preparative Guard Cartridge is available in dimension of 10x20 mm I D and 10x30 mm I D with various functional groups In principal the same material and the same inner diameter as main analytical column is recommended Guard cartridge holder ae 3 Usage STEP 1 Assembly of Semi Preparative Guard Cartridge Insert the cartridge column into the holder The guard cartridge is direction free Tighten by hands as much as possible Then use wrench sizes mentioned below Tighten as a guide by about 90 135 degrees about 6 9 tick marks In the case where leakage is occurred please tighten further Required wrench size is 19 mm Place a wrench on hexagonal protruding parts on both inlet side and outlet side and tighten while holding the holder body Do not over tighten when you tighten further or it may damage cartridge column The constructed guard cartridge is connected to the main column inlet
2. with column coupler Small inner diameter and short length tubing is recommended Guard cartridge column The flow direction of the guard cartridge should not be changed after the first connection Re connection in reverse direction may cause the impurities adsorbed at inlet side of the Guard Cartridge to flow out to main column Incase of leakage tighten holders until the leakage stops STEP3 Replacement of cartridge column The replacement time of the guard cartridge column can be judged by the increase of pressure the change in peak shapes and resolution Repeated injection of a large amount of sample may result in loss of adsorption ability of guard cartridge column In such case impurity to be trapped by guard cartridge column may flow into the main column Early replacement of the cartridge column is recommended to prevent contamination of main column Replacement YMC CO LTD YMC Karasuma Gojo Bldg 284 Daigo cho Karasuma Nishiiru Gojo dori Shimogyo ku a i Kyoto 600 8106 Japan TEL 81 75 342 4515 FAX 81 75 342 4550 URL www ymc co jp E Mail sales ymc co jp UM131002AE

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