LHC4011 Lot 731602 - Thermo Fisher Scientific
Contents
1. 3 Part SM311 Description Hu 16 Plex Standard Lot 621958 Size 2 Vials Form Lyophilized The proteins in this standard have been calibrated against the mass of highly purified recombinant protein with the respective Invitrogen ELISA kit and NIBSC calibration standard if available Refer to the User Manual included in the Buffer Reagent Kit for further information Contains 0 1 sodium azide as a preservative Storage Store at 2 to 8 C Use within 1 hour after reconstitution Discard immediately after use Concentration of Reconstituted Standards IL 1B 5 900 pg mL IL 10 15 400 pg mL IFN o 7 900 pg mL IL 6 3 700 pg mL IL 12 7 900 pg mL IL 13 8 700 pg mL IL 15 25 300 pg mL IL 17 12 000 pg mL GM CSF 14 600 pg mL IL 5 3 200 pg mL IFN y 13 300 pg mL TNF a 5 200 pg mL IL 2 13 600 pg mL IL 7 10 100 pg mL IL 4 3 600 pg mL IL 8 12 400 pg mL Important note The concentrations of reconstituted standards are lot specific Please verify all concentration values entered in data analysis software Reconstitution Reconstitute with 1 mL Assay Diluent when measuring IFN o in serum or plasma samples For other sample types such as tissue culture supernatants reconstitute the standard in 1 mL of a solution consisting of 50 Assay Diluent 50 of sample matrix Allow standard to rehydrate for approximately 10 minutes before further dilution Recommended Starting Concentration for Standard Curve Upon reconstitutio2. invit rogen Human IFN a Singleplex Bead Kit Technical Data Sheet Catalog LHC4011 Lot 731602 Note A letter at the end of the lot number signifies an additional packaging of this same lot Intended Use This kit is comprised of components for the measurement of human IFN a in plasma or tissue culture supernatant The assay may be run alone or in combination with other Singleplex Bead Kits from Invitrogen Buffer reagents needed to complete the reaction are sold separately under Catalog LHB0001 These reagents are intended for use in the Luminex 100 or 200 System only This kit is configured for research use only and is not to be used in diagnostic procedures Reagents Provided Hu IFN a l Part LM071 Description Antibody Bead Concentrate 10X Lot 731604 Size 0 25 mL 100 tests Bead Region 17 Form 0 25 mL 10X bead concentrate solution in storage buffer Contains 0 05 sodium azide as a preservative Storage Light sensitive material Store at 2 to 8 C in the dark until the expiration date indicated on the kit Hu IFN a 2 Part BNO71 Description Biotinylated Ab Conc 10X Lot 731603 Size 1 mL 100 tests Form 1 mL of a 10X stock of Biotinylated Antibody Concentrate in Biotin Diluent Contains 0 1 sodium azide as preservative Concentration of antibody is matched to this lot of beads Do not mix lots of Coated Beads and Detection Antibody Storage Store at 2 to 8 C until the expiration date indicated on the kit
3. 012 Tel 800 955 6288 E mail techsupport invitrogen com PILHC4011 Rev 01 10 Page 2 of 2 DCC 10 0198 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use
4. IFN alpha Low End Range 2 2 Z gt gt 5 5 S S i i 0 2000 4000 6000 8000 0 50 100 150 200 250 300 Concentration pg mL Concentration pg mL Typical Standard Curve Specificity Buffered solutions of a panel of substances at 5 or 10 ng mL were assayed with the Invitrogen Human IFN o Singleplex Bead Kit The following substances were tested and all were found to have no cross reactivity human IL 1 IL 2 IL 4 IL 5 IL 6 IL 8 IL 10 IL 12 IL 13 IL 15 EGF Eotaxin FGFb G CSF GM CSF IFN y MCP 1 MIP la MIP 1B RANTES TNF a and VEGF This Kit will recognize the following human interferon species IFN aA IFN a2 IFN aA D IFN aC IFN aD IFN aG IFN oJ and IFN ak The following human interferon species are not recognized by this Kit IFN aB2 IFN aF IFN aH IFN al IFN B IFN y and IFN o Precision Intra assay Inter assay n 24 n 46 Mean pg mL 2045 2089 SD 139 166 Y CV 6 8 7 9 Linearity Tissue culture medium containing 10 fetal calf serum was spiked with human IFN a and serially diluted in a solution of 50 Assay Diluent and 50 tissue culture medium Linear regression analysis revealed a correlation coefficient of 0 99 Recovery Human serum averaged 93 range 71 to 105 Human EDTA plasma averaged 98 range 95 to 101 Human citrate plasma averaged 89 range 84 to 94 Human heparin plasma averaged 92 range 88 to 94 Tissue culture medium containing 10 fetal calf serum aver
5. aged 99 Correlation to ELISA A correlation coefficient of 0 94 was calculated when values for tissue culture medium obtained with the Human IFN a Singleplex Bead Kit were compared to the Invitrogen IFN a ELISA cat KHC4012 Human IFN a Singleplex Bead Kit pg mL x 1 5 Hu IFN a ELISA pg mL Correlation of results obtained with the Human IFN a Singleplex Bead Kit to one s own system should be determined to arrive at an appropriate multiplication factor Explanation of ee Research Use Only In vitro diagnostic medical device oA EC REP European Community authorised representative D without does not contain With comtains contains Protect from light Consult accompanying documents Q Directs the user to consult instructions for use IFU accompanying the product By purchasing this Kit which contains fluorescently labeled microsphere beads authorized by Luminex Corporation Luminex you the customer acquire the right under Luminex s patent rights to use this Kit or any portion of this Kit including without limitation the microsphere beads contained herein only with Luminex s laser based fluorescent analytical test instrumentation marketed under the name Luminex 100 or 200 This product is covered by one or more of the following U S patents 6 046 807 For research use only CAUTION Not intended for human or animal therapeutic or diagnostic use Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93
6. n the starting concentration of standard is the value cited above Make serial 1 3 dilutions in Assay Diluent serum plasma samples or other appropriate matrix Use 100 uL per assay If establishing a Multiplex Assay this same standard can be used to measure the other related cytokines cited above in a Multiplex Assay format Refer to the User Manual included in the Buffer Reagent Kit for further information For research use only CAUTION Not intended for human or animal therapeutic or diagnostic use Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PILHC4011 Rev 01 10 Page 1 of 2 DCC 10 0198 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use Human IFN a Singleplex Bead Kit Technical Data Sheet Performance Characteristics Analytical Sensitivity The minimum detectable dose of Human IFN o is lt 15 pg mL This was determined by adding two standard deviations to the mean FI obtained when the zero standard was assayed 24 times Human IFN alpha Full Standard Range Human
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