here - Mystrica
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1. Press the power switch and select the desired colour of light by pressing RGB Select absorbance or transmittance by pressing A T Insert a cuvette containing the blank in the colorimeter Make sure the clear faces are aligned with the light path Press CAL The display will show CAL for a few seconds then either GUG A or 00 0 T Taking readings Replace the blank with a cuvette containing the liquid to be measured close the cap and read the value from the display In stand alone mode the colorimeter will switch off automatically after 2 minutes to conserve battery power The settings are retained so to take a reading simply press the power switch and continue working After use Switch off by disconnecting from the app or USB or by pressing the power switch Check inside the cuvette holder and wipe up any spills with a rolled up piece of absorbent paper Wireless connection The app Mystrica Colorimeter is free from the Apple App Store and can be used with iPads Air Mini 3 amp 4 generation and Colorimeter iPhones 4S and later not 4 When the app is opened the Setup screen appears Setup screen see facing page The name of every device switched on within range will be shown and one can be selected Each colorimeter is supplied with a unique identity the Device Name This can be changed by anyone in possession of the password after connecting using the Set Name tag Name2. available free from the Apple app store iPads Air Mini 3 amp 4 gen and iPhones 4S and later not 4 e with PCs running Windows using the free software download The uses are primarily in secondary and tertiary science education particularly for Biology and Chemistry Detailed suggestions for practical activities can be found at www mystrica com and include e rates of chemical reactions determination of rate order e investigations with enzymes effects of temperature pH inhibitors enzyme kinetics e quantitative determination of biological molecules sugars protein vitamins etc e quantitative determination of inorganic ions nitrate phosphate etc e population growth of micro organisms e and others Package Contents O Mystrica Colorimeter O Cuvette Stand O 10 Cuvettes O 10 Cuvette Caps O 2x12mm Test tubes O USB Cable A to mini B O Device Name Label O Password O Laminated Instructions O User Manual Routine Maintenance e After use check that the cuvette holder is empty and that nothing has been spilled inside it e In the event of a spill mop it up with a cotton bud or a rolled up piece of absorbent paper e Wipe the outside with a damp cloth to remove spills and stains Device Name Each colorimeter has a unique name that is used to identify it to the app when the wireless function is in use This is initially set to correspond to the serial number on the rear label but can be changed
3. 2 0 848 14 6 0 838 14 9 0 828 13 The Graph screen see facing page The axes scales change automatically to accommodate the data as they are collected y axis is either Transmittance or Absorbance x axis default is time in seconds but it can be changed to record any numerical values eg concentration by entering numerical values using the Note tag see page 16 Best Fit Line Tapping any point on the graph highlights it Once two or more points have been selected the app calculates the best fitting straight line through the points draws the line and displays the values in the form y ax b where a is the gradient of the line ie change in Absorbance or Transmittance per second or whatever units are on the x axis see page 16 and b is the intercept on the y axis 14 10100 M Email 0 030 Separate Event Calibrate Best Fit Line y 0 0090x 1 0163 15 Separate events When the Separate Event tag is tapped only the current reading is recorded in the results table The Note tag Anything entered in this box will appear in the Note column of the results table It can be used to record details of the experiment to identify the person collecting the results to record reaction conditions etc If a number is entered in the Note box this will be shown on the graph x axis This allows a graph to be constructed of absorbance against concentration or anyt
4. by anyone in possession of the Password see page 10 light path mm Write the device name here AVOID SPILLS and attach the label to the USE CUVETTE CAPS front of the colorimeter The security level for the Device Name is not high The purpose is to allow each colorimeter to have an easily recognised name while trying to avoid unauthorised alterations by restricting knowledge of the Password 3 Safety The Mystrica colorimeter is a robust instrument but can be damaged by severe spills if these penetrate to the electronics Avoid these by using cuvette caps Minor spills will be trapped in the well at the bottom of the cuvette holder and can easily be removed with a cotton bud or rolled up piece of absorbent paper AVOID SPILLS USE CUVETTE CAPS Wipe the outside of the cuvette before putting it in the colorimeter DO NOT PUT ORGANIC SOLVENTS SUCH AS PROPANONE OR ETHANOL IN PLASTIC CUVETTES USE TEST TUBES OR GLASS CUVETTES FOR THESE The readings on the colorimeter can be affected by changes in temperature Avoid placing the unit in direct sunlight or working in an environment subject to large changes in temperature lf you are using the colorimeter in such an environment you should recalibrate frequently Controls Da tria a Oooo Pressing the power switch turns the unit on or off In stand alone mode the unit switches off after 2 minutes to conserve battery power When the wireless or USB connections are i
5. numerical value or with text If numeric the data captured can be displayed as a graph with the numeric values on the x axis If text the readings will only appear as a table When you click Take Readings only the current value from the colorimeter will be recorded as a single discrete entry Manual Entry In the Separate Events box Separate events there is the option to enter both the X axis values and Name your sequence Untimed results 27 Oct 20 the colorimeter readings ie x without using the current readings from the colorimeter Automatic entry Manual entry Description Drawing lines of best fit A straight line of best fit can be drawn through any number of points on a graph Select the points by clicking them then click Draw Best Fit Line The equation will be displayed in the form y ax b where a is the slope of the line and b is the intercept of the line on the y axis More information about using the software can be found at www mystrica com Software 23 Federal Communications Commission Statement This device complies with part 15 of the FCC rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation NOTE The manufacturer is not responsible for any radio or TV interference caused by unauthorized modifications or chan
6. D 2AC8AMYS2 Email enquiries mystrica com Copyright 2014 Mystrica Ltd 24
7. Mystr LCA 20 wireless colorimeter _ D SPILLS USE CUVETTE CAPS User Manual Index ADDIGAHONS lied Package contents Routine Maintenance Device Name cli lille D a CONHOIS mea SNS A ea Ga BISHA sus nt tn ra POWSFSUPDIV scsi ae Cuvettes and Test tubes How the colorimeter works How to use the colorimeter Wireless ADD sil ile Setup screen Table S r eri ssi Graph SCFeel H nin Best FILING rile Separate events The Note tag Saving results USB connection to a PC PG Software iui nile System requirements Using the software Creating a new project _ Creating a new experiment __ Continuous readings Displaying results as tables or graphs Separate events Drawing lines of best fit Conconi agere Applications The colorimeter is suitable for educational use It is not intended for clinical commercial or research applications It can be used e as a stand alone instrument e with tablet computers and phones using the iOS app
8. d with the colorimeter Use glass test tubes or glass cuvettes for organic solvents see Safety page 4 How the colorimeter works The light source is one of three light emitting diodes LEDs which provide Red 630nm Green 525nm or Blue 465nm light The light passes through the sample in the cuvette and is detected by a photodiode Transmittance is a measure of the intensity of light passing through the sample or an indication of the amount of light energy passing through There is a simple linear relationship between transmittance and intensity which can be expressed as T where I is the intensity of the light entering the sample and is the intensity of light coming out of the sample This value is usually given as percent Absorbance is defined as logio lo or 2 logioT There is a linear relationship Beers Law or the Beer Lambert Law between absorbance and the concentration of the light absorbing substance in the sample In fact absorbance is determined by concentration and by the length of the light path through the sample For most experiments in biology and chemistry the linear relationship with concentration makes absorbance the measurement of choice How to use the colorimeter Calibration This sets the value of a predetermined solution the blank to 100 transmittance or an absorbance of 0 The blank would usually be pure water though this is not necessarily the case
9. e Timed Results 20 Dec 2008 11 09 55 Experiment we arte Description Cancel When Next is clicked the box shown below appears Use this to enter the length of time you want to collect data for Hours Minutes Seconds and the interval between data points Timed Results 20 Dec 2008 11 09 55 JS Automatic entry set up Time to run data capture for fo H fo M fo 5 ee Capture data every fo Secs lt lt Prev Cancel Click Next then on the next page click Take Readings to start collecting data 21 Displaying results as Tables or Graphs The data can be displayed as a table or graph except when text is entered using Separate Events In graph view you can select to display the y axis as either Transmittance or Absorbance and to join the points with straight lines curves or not at all Data collection can be stopped prematurely discarded saved or exported as a csv file to a selected location by clicking the labelled buttons Select Grid or Graph view Draw Best Fit Line Transmittance x Dont join points gt Transmittance Graph as Absorbance or Transmittance Click here to take readings Take Reafings 000 01 00 lt lt Prey Next gt gt Cancel 22 Separate Events The procedure for Separate Events is similar to that for Continuous Readings except that you will be asked to select whether the readings should be associated with a
10. e click the project folder and experiment files to view these Y S Project folder New Project 19 Creating a new experiment Right click on the project folder and click New Experiment The box shown below will appear and a title and any other information can be entered The experiment file can contain any number of sequences of results collected from the colorimeter Mises 16 48e LA u eee 2 ee IE 8 EN al Le 8 SE Name CUT RAT ET L ye nates PESCriptION u maha r Save Cancel P ted apia perdi alii ind da Preparing to collect data Right click the experiment file The box shown below will appear Delete Experiment Rename Experiment New Continuous Reading Mew Seperate Events Selecting New Continuous Reading allows data to be collected in a continuous stream for however long is set New Separate Events allows capture and storage of only the current value from the colorimeter 20 Continuous readings When New Continuous Reading is selected the box shown below appears You can enter a name for the sequence of results you are about to collect the data and time are optional but it is usually good practice to leave these The Description box can be used to give details of the sequence concentrations of reactants temperature pH etc Take Continuous Readings Automatic Enty C Manual Enty v Name your sequenc
11. ges to this equipment Such modifications or changes could void the user s authority to operate the equipment NOTE This equipment has been tested and found to comply with the limits for a Class B digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different from that to which the receiver is connected e Consult the dealer or an experienced radio TV technician for help The Mystrica colorimeter 2 0 has been tested and found to comply with harmonised standards EN 60950 1 2006 A1 1 2009 A1 2010 A12 201 1 EN 301 489 01 V1 9 2 2011 EN 301 489 17 V2 2 1 2012 EN 300 328 V1 8 1 2012 Test certificates can be viewed at www mystrica com FCC I
12. hing else that has a numeric value This works if only numbers are entered otherwise the x axis is shown as the default setting time in seconds Saving results To save data tap the Email tag The contents of the current table can be sent to any email address as a csv file and displayed or analysed in whatever data analysis programme is preferred There are a number of short video clips illustrating the operation of the app on the website www mystrica com 16 USB connection to a PC The colorimeter connects to a computer through the mini USB connector shown The power switch turns the unit on or off PC Software The software is FREE to DOWNLOAD see www mystrica com Software Download Using the colorimeter connected to a computer by the USB link you can set up project and experiment files to collect and store results display and store data as tables and graphs collect data as a continuous stream or as separate events carry out simple data manipulations such as calculating rates of reaction and lines of best fit copy data into more sophisticated data analysis applications such as Excel or Autograph 17 System requirements Windows 2000 XP Vista Windows 7 or 8 with the NET Framework 2 0 or later installed To install the software follow the on screen instructions Using the software Instructions and video demonstration clips can be found on the website www mystrica com Software P
13. n use the unit stays on unless switched off by pressing the power switch e CAL calibrates the unit to 0 000 Absorbance or 100 0 Transmittance e RGB switches between the red green and blue light sources e A T switches display between Absorbance and Transmittance Display _ es as tei iene oloni a edi e Indicates which colour of light has been selected Red Green or Blue e Indicates whether the unit is displaying values as Absorbance or Transmittance e Displays values of transmittance between 0 0 and 100 0 and absorbance values between 000 and 1 999 The software for PC tablet or phone can show higher values and negative values Non numeric readings The display cannot show negative values or values greater than 1 999 Absorbance or 199 9 Transmittance The following non numeric displays may be encountered e IDO the absorbance is negative i e less than the absorbance of the solution used for calibration Recalibrate using the least absorbent solution usually water e Hi the absorbance is higher than 1 999 a reading may still be available with the computer software H 4 transmittance is higher than 199 Recalibrate e FAL the batteries are low and should be replaced This should be a very rare occurrence Power Supply The unit uses 6V from four AA batteries These are inserted by removing the screw on the battery holder When the batteries need to be replaced the di
14. ress the power switch on the colorimeter to switch on The programme screens display information as shown below colorimeter settings and value Lidi xP a J tenda Agg MIM 14 1 TED cage of gt fr 3 1D p tare hl ps 29442008 ae Gi fi Gi Vins Graph View fr DrawfestFtUre w Dont jan port nn pue 0 Pali ui list of experiments and Fu result sequences inthe displayed as t current project folder or graphs a 10 2 20 4 ss eo m eo 90 10 HO 120 132 M 19 16 i 18 Creating a new project project folder contains a set of related experiments Each experiment can contain any number of sequences of data The name of a project could be for example the name of the person whose work this is or a general title for a set of related experiments The first time the programme is started an empty project folder is opened called Colorimeter Project right click on this folder to rename it Subsequently the programme will always open with the project folder that was last used The Files option on the menu bar can be used to create a new project to select and open an existing project and to save the current project Mystrica Colourimeter new proje File Take Readings Change Language The left side of the screen displays below the current readings and settings from the colorimeter a list of the experiments and data sequences contained in the project folder doubl
15. s must be between 4 and 10 characters Before connecting select the Logging Interval This will be the time between recorded readings when the colorimeter is collecting continuous data 1 5 or 30 seconds Tap Connect The colorimeter must be switched on When a connection has been successfully established the Connect tab will change to Disconnect Tap Disconnect to switch off the colorimeter or press the power switch Select Setup Table or Graph from the bottom of the screen 10 myst02 Connect Logging Interval 1 Second 11 The Table screen see facing page The transfer of information between app and colorimeter is two way Tapping the screen changes the colorimeter settings Changing the settings on the colorimeter changes the screen display Shows the current reading on the colorimeter Tap to calibrate Tap to select Red Green or Blue light Tap to select Absorbance or Transmittance Tap to start and stop continuous data collection Tap to clear all data and reset the time to zero Tap to collect only the current reading see page 16 Tap to type in notes see page 16 Tap to save results by emailing them as a csv file 12 100 1 Separate Event Calibrate Note 18 Time Transmittance Absorbance Colour Os 12 4 0 909 B 12 6 0 899 B 12 9 0 889 13 2 0 879 13 6 0 869 13 9 0 858 14
16. splay will show Ht on startup Battery life for 1000mAh AA batteries is estimated at 100 hours of continuous use Battery replacement should be a very rare event When connected to a computer through the USB link the unit draws its power through the link and is permanently on Cuvettes The samples to be measured are placed in standard 4 5ml glass or plastic cuvettes The length of the light path is 10mm Satisfactory results can be obtained with volumes down to 1ml For lower volumes semi micro cuvettes can be used but the unit is not intended for semi micro or micro applications Some cuvettes have four clear faces Many have two clear faces and two frosted or ribbed faces The clear faces must be aligned with the light path i e across the unit parallel to the display The unit is supplied with ten plastic cuvettes with lids and one cuvette stand Additional cuvettes lids and cuvette stands are available through www mystrica com Test tubes 12mm test tubes fit the cuvette holder and will give good results though cuvettes are probably more accurate Note that older versions of the colorimeter pre wireless version 2 0 are unsuitable for use with test tubes because the cap must be in place to prevent external light entering The new colorimeters have a brighter light source and are less prone to interference from external light although this should be guarded against in very bright conditions 2 x 12mm test tubes are supplie
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