CytoBrite System User Manual
Contents
1. 2020 00 1 CytoBond Removable Coverslip Sealant 100 ml 2022 00 2 CytoZyme Stabilized Pepsin 50X Concentrate 20 ml gt 2022 00 3 CytoZyme Stabilized Pepsin 50X Concentrate 100 ml 2022 10 2 CytoZyme Reaction Buffer 1L 2022 10 3 CytoZyme Reaction Buffer 4L 2030 00 1 Sodium Thiocyanate Pretreatment Reagent 1L 2030 00 2 Sodium Thiocyanate Pretreatment Reagent 4L 1051 52 0 Digital thermometer Includes cable and NIST certificate mimimim gt gt gt gt Version 1 1C SW 2 0 August 2015 20 408 733 7337 techserv scigene com SciGene XIII DECLARATION OF CONFORMITY CytoBrite Slide Incubation System SciGene 1287 Reamwood Ave Sunnyvale CA 94089 USA CytoBrite Slide Incubation System USER MANUAL CE Declares that the above referenced product s meets the essential requirements of the following European Union Directives by using the relevant standards shown below to indicate compliance EMC Directive 2004 108 EC EN 61326 1 EN 55011 EN 61000 3 2 EN 61000 3 3 EN 61000 4 2 EN 61000 4 3 EN 61000 4 4 EN 61000 4 5 EN 61000 4 6 EN 61000 4 8 EN 61000 4 11 LVD Directive 2006 95 EC IEC 61010 1 RoHS Directive 2011 65 EU Terry Gill Name of Authorized Representative Director of Product Manufacturing 2013 2010 2009 2013 2009 2006 2012 2006 2014 2010 2004 2013 Title of Authorized Representative ptl Signature of Authorized Representative Version 1 1C SW 2 0 Au2. Operating Mode 3 When the preheat step is complete the display will flash Load Slides Press Select Open the lid load the tray s and press Select OneTemp Protocol RUN 5 Ifa denaturation step is part of the protocol FiS Location the unit will rapidly heat and hold at denature temperature then rapidly cool to incubate temperature warming for a preset time as programmed by the operator CytoBrite RUN Select File 1 File100 2 File200 6 Ifa denaturation step is not used the unit will simply heat to the incubate temperature where the slides will be warmed for the programmed time RUN Run Protocol File100 Yes 7 f Off was selected as the program ending option slide block heating will turn off at the completion of the protocol If Hold was selected the slide block will be held at the programmed temperature RUN File100 Preheat 36 9 Temp 37 Time 00 05 00 Preheats for 5 minutes at programmed temperature RUN Preheat Complete Load Slides Press Select If Denaturing Incubating Only Press any key to terminate a protocol Select Yes in the Turn Off Block confirmation screen to return to the Home screen Unit beeps to load slides RUN File100 RUN File100 Mode Denature Mode Incubate Temp 72 9 Temp 37 1 37 Time 00 05 00 Time 16 00 00 File100 DONE File100 DON
3. Sealant around edges 3 Turn on power to the instrument select and start the desired program See section V for how to create programs 4 When the unit finishes pre heating the controller displays a prompt and beeps to load slides Raise the cover place the tray s into the unit and press Select to start the program 5 The instrument then heats the slides to the programmed temperatures and times C Powering On Off ay To turn ON the instrument press the top of the power switch the I position To turn OFF the instrument press the bottom of the power switch the O position D Using the Controller A 4 line LED display controller is used to CREATE RUN EDIT and VIEW protocols manage FILES rename copy delete and customize SETTINGS including calibrating temperature and choosing alert sounds Navigating menus and selecting options is performed with four buttons on the display panel e Home returns to the main menu screen D D D D DH HOME D SELECT e Select chooses the option highlighted in the display e SciGene e Forward increments or toggles between options e Back returns to a previous screen or option Details on using controller menus can be found in later a sections of this manual E Using the USB Drive A USB key inserted into the USB DRIVE can be used to x CytoBrite e Capture a time and temperature log when running a Slide Incubation system protocol e Allo
4. er un risque de s curit F Compliance C 1 European Conformity CE This symbol indicates the instrument is in compliance with all applicable European Union Electromagnetic Compatibility and Low Voltage Directives 2 Waste Electrical amp Electronic Equipment Directive WEEE This symbol indicates the instrument is required to comply with the L a European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC providing environmentally safe disposal of end of life equipment through recycling Contact your institution to provide environmentally safe disposal If this is not available contact your local SciGene distributor or SciGene Technical Support techserv scigene com for assistance Do not treat electrical and electronic equipment as unsorted municipal waste ROHS 3 Restriction of Hazardous Substances RoHS This symbol indicates the instrument is in compliance with the European Union s RoHS 2 Directive 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment EEE Version 1 1C SW 2 0 August 2015 5 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL 4 Electromagnetic Compatibility EMC The CytoBrite System is a Class A digital device under FCC Title 47 Part 15B and designated as Class A electrical equipment for measurement control and laboratory use EN61326 Note
5. on the controller then press Select The controller will now display temperature using new offset Compare the new controller temperature to the thermometer the temperatures match each other to within a few tenths of a degree press Yes to save the new offset If not press No to enter a new offset value Once the new offset is confirmed the instrument will automatically cool to 37 C When the temperature reaches 37 C on the controller display wait 5 minutes Repeat steps 4 through 6 to determine a new offset value at 37 C Confirm the second offset to return to the Settings menu Calibration is complete f CytoBrite Run Create View Edit Settings Files SETTINGS 1 Datalog 2 Calibrate 3 Sounds 4 Upgrade SW Instrument automatically heats to 75 C Adjust offset to match digital thermometer SETTINGS Calibrate 75C Temp 75 0 Offset 4 2 SETTINGS No Temp 75 0 Offset 4 5 Save Yes SETTINGS Calibrate 75C Offset Saved Instrument automatically cools to 37 C SETTINGS Calibrate 37C Temp 37 Offset 4 0 Adjust offset to match digital thermometer SETTINGS No Temp 37 0 Offset 3 8 Save Yes SETTINGS Calibrate 37C Offset Saved Return to Settings 408 733 7337 techserv scigene com SciGene C Upgrading Software The CytoBrite System displays the current software version on the controller when powe
6. regarding Canadian EMC compliance Le present appareil numerique n emet pas de bruits radioelectrique depassant les limites applicables aux appareils numeriques de class A prescrites dans le reglement sur le brouillage radioelectrique edicte par le Ministere des Communications du Canada FCC Warnings and Notes This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules The instrument qualifies as an exempted device under 47 CFR 15 103 c in regard to the cited FCC regulations in effect at the time of manufacture Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits Version 1 1C SW 2 0 August 2015 6 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL ll UNPACKING AND SET UP A Unpacking the Instrument The CytoBrite instrument is shipped in a double carton To unpack the instrument open the outer box take out the bag containing the manual and power cord and remove the protective cardboard corners Then open the inner box reach down the inside and carefully lift out the instrument by its base NEVER lift by the hinged lid Place the instrument on the bench and raise the lid to remove two slide trays Inspect parts for visible damage If any damage is evident retain all shipping materials and contact SciGene techserv scigene com or your local distributor P
7. screen by pressing the Back button twice CytoBrite Slide Incubation System USER MANUAL Upgrading Software CytoBrite Create Edit Settings SETTINGS 1 Block Type 2 Calibrate 3 Upgrade SW y 4 Sounds SETTINGS Upgrade Software Insert USB press Select Upgrading kkkkk kk kk kkk kk kk kkkkkkkkkkkkkk Upgrade Complete Press any key SciGene Version x x Month Day Year Return to Settings Changing Sounds CytoBrite Create Edit Settings SETTINGS 1 Block Type 2 Calibrate 3 Sounds 4 Upgrade SW SETTINGS Beep After 1 Protocol End Off A 2 Each Select Off V 3 Preheat Off 4 Denature Off SETTINGS Beep After Each Select et On SETTINGS Beep After 1 Each Select On A 2 Each Step Off V Return to Settings 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL IX MAINTENANCE A Cleaning the Instrument Clean outer metal surfaces and the heated lid using a soft cloth and mild detergent based cleaner Avoid abrasive cleaners that can scratch surfaces Do NOT use caustic or strongly alkaline solutions e g strong soaps ammonia or bleach at a higher concentration than specified above If running radioactive or biohazardous reactions consult your institution s radiation safety officer or biosafety office for assistance Turn the power switch to the OFF position and un
8. with the sale use or performance of the product Copyright Copyright 2014 2015 SciGene Corporation All rights reserved SciGene CytoBrite and CytoBond are trademarks of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners Version 1 1C SW 2 0 August 2015 1 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL Table of Contents k SAFETY eh te E 3 A SO de ae eo a 3 Be CRE ST a a ee ne a nc eo E 3 G SVB EE el CONVENON sisiy EE 3 D ge 4 E CON eo sci canncasieceucoasnaceaoceacdes acweccoven soner aia seaseautenasaoarteans coq wanscauuacassewensamsccsiean oarnennacueasiean 5 F Balo AG EE 5 lE UNPACKING AND SETUP E 7 A Unpacking the Eu LEE 7 ME POI EE 7 Gs e ET gdan POV ee ae eee 7 D Environmental Requirements ccccccssssseecccceesececceeeecccceaeseceessauseceeseseeueeccesaeseeeessseueeeesseaseseaaeceesauaaeees 7 E IO elef et SPA BR aa E NET E ER 7 IDWEOllcadelfl egtel RSC CH 8 iere 8 B Preparing Slides Loading the Instrument Starting a Protocol cccccccccccccccccesssssssssssseeeeeeeeeeeeeeeeeeeeeees 9 C Glen EE 9 D Uneme COMORES aa A D ee a en 9 E Bt ne Re 9 IV CREATING AND EDITING PROTOCOLS seems nent eanenssamnse tendres ere se eee 10 Pee Cne PG e E 10 B Editing a e e e e RE 11 Y ONETEMP MODE iiccaisssescrsensicaccanncossastiasnweasnacasienacnnues cee acnannensausn
9. 0000 www scigene com CytoBrite Slide Incubation System USER MANUAL Cat 2019 00 1 115 230V FOR RESEARCH USE ONLY 1287 Reamwood Avenue Sunnyvale CA 94089 USA e Tel 408 733 7337 Fax 408 733 7336 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the CytoBrite Slide Incubation System described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable slide trays or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to at its sole discretion a provide replacement parts b perform factory repairs or c replace the product The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damagers or for any damages resulting from loss arising out of or in connection
10. 1C SW 2 0 August 2015 15 408 733 7337 techserv scigene com SciGene B Calibrating Slide Block Temperature CytoBrite Slide Incubation System USER MANUAL The CytoBrite System comes calibrated from the factory to provide accurate slide temperatures from 15 to 90 C 0 5 C Temperature accuracy should be checked periodically according to your lab schedule with a T type NIST certified digital thermometer SciGene cat 1051 52 0 Temperature Calibration The system is calibrated by entering offset values at 75 C and 37 C as follows 1 Version 1 1C SW 2 0 August 2015 16 Using the cable supplied with the T type NIST certified digital thermometer SclGene cat 1051 52 0 connect it to the blue jack on the rear of the instrument labeled Temperature Calibration and turn on power to the CytoBrite System From the Home screen go to the Settings menu and select Calibrate The instrument will automatically heat to 75 C When the temperature reaches 75 C on the controller display wait 5 minutes To determine the offset value subtract the controller temperature from the thermometer temperature For example e Ifthe thermometer reads 75 5 and the controller reads 75 0 add 0 5 to the existing offset value e Ifthe thermometer reads 74 5 and the controller reads 75 0 subtract 0 5 from the existing offset value Enter the new offset value in the Calibration screen by using the Forward or Back buttons
11. 7337 techserv scigene com D SC G e n e CytoBrite Slide Incubation System USER MANUAL lil USING YOUR CYTOBRITE SYSTEM A System Components SlideSense Temperature Sensor Senses and transmits slide block temperature information to controller Temperature Controller Used to program and monitor instrument operation and report slide block temperature Slide Tray Holds slides during sample preparation and incubation USB Port Used for saving datalog files and installing software Hinged Lid Covers slide trays during processing calibrate slide block temperature Power Input Module amp Fuse Housing CytoBond Removable Coverslip Sealant AN d a d 7 WW Block Temperature Calibration Jack Temperature Controller USB Port Power Switch Version 1 1C SW 2 0 August 2015 8 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL B Preparing Slides Loading the Instrument Starting a Protocol Slides are prepared for molecular analysis following your standard procedures using the CytoBrite Slide Trays provided with the unit CytoBond Removable Coverslip Sealant SciGene cat 2020 00 1 is used in place of rubber cement for sealing coverslips to prevent probe evaporation without humidification 1 Place slides to be analyzed in the tray s pipet probes and place coverslips 2 Seal coverslips by dispensing a continuous bead of CytoBond
12. E Time 16 00 00 Time 16 00 00 Temp Off Temp 37 1 File100 DONE Tum off block retum to home Yes No Return to Home screen Version 1 1C SW 2 0 August 2015 13 408 733 7337 techserv scigene com SciGene Vil MANAGING FILES A Viewing a Protocol CytoBrite Slide Incubation System USER MANUAL Version 1 1C SW 2 0 August 2015 14 Saved protocols can be reviewed in read only mode 1 Select View from the Home screen 2 Choose the file location controller memory or USB key and protocol 3 Use Forward to advance through the protocol in the following order e Denature temperature and time e Incubate temperature and time e Final hold temperature or OFF 4 Press the Forward or Back buttons to continue Copying a Protocol A protocol can be copied and saved under a new name in the controller memory or USB key CytoBrite Run Create View Edit 1 Select Files from the Home screen Files Settings 2 Choose the file location controller memory or USB key and protocol FILES File Location 3 Select Copy CytoB rite USB Key Choose new file location and Select Yes FILES CytoB rite Folder Select File 1 37to4 Renaming a Protocol An existing file may be renamed and saved in the controller memory or USB key FILES CytoB rite Folder File 37to4 Copy Name Delete Copy Select Files from the Home screen Choose the file location co
13. EDITING PROTOCOLS 1 From the Home screen choose Run OneTemp 2 Set the desired block temperature from 15 to 90 C 3 Press Select Yes to start the program Set temperatures for the block will be displayed Time will be listed as Forever as the program will continue until stopped by the user 4 To end the program use the Forward button to display the End Protocol screen and Select Yes The instrument will display OneTemp DONE and total elapsed time CytoBrite Create Edit Settings Operating Mode OneTemp Protocol RUN OneTemp Set Temp 73C Temp 37C Time Forever Start Yes No RUN One emp Temp 73 2 73C Time 05 00 00 End OneTemp OneTemp DONE Time 00 01 01 Return to Home screen Version 1 1C SW 2 0 August 2015 12 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL VI RUNNING A PROTOCOL The RUN function is used to run a previously created protocol stored in the controller memory or on a USB key To operate the CytoBrite instrument at a single temperature without a timer see section V ONETEMP MODE 1 Select Run Protocol from the Home screen Running a Protocol 2 Choose the file location CytoBrite or USB key CytoBrite ie and select the protocol The protocol will start by View Edit performing a 5 minute preheating of the block ists pean to the programmed incubation temperature RUN
14. ain POWER switch Ce symbole indique la position OFF de l interrupteur principal This symbol indicates the ON position of the main POWER switch Ce symbole indique la position ON de l interrupteur principal 2 Non Electrical Symbols A D Warnings CAUTION This symbol illustrates a heat hazard Proceed with caution when working around these areas to avoid being burned by hot components ATTENTION Ce symbole illustre un danger pour la chaleur Faire preuve de prudence lorsque vous travaillez autour de ces zones pour viter d tre br l par les composants du chauds CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution ATTENTION Ce symbole vous avertit consulter ce guide de l utilisateur pour plus d informations et de proc der avec prudence Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death D faut de respecter les avertissements suivants qui sont appos es sur le produit peut conduire possibles l sions corporelles ou la mort A This symbol on the rear of the instrument indicates the presence of the fuse box Warning For Continued Protection Against Fire Replace Only with Same Type Rating of Fuse Always disconnect the power cord before attempting to replace the fuse Ce symbole sur l arri re de l instrument indique la pr sence de la bo te de fusibles Av
15. arts Provided e 2x Slide Trays shipped inside instrument e Power Cord e User Manual Coverslip Sealant Provided One complimentary 100 ml bottle of CytoBond Removable Coverslip Sealant SciGene cat 2020 00 1 is shipped separately from the instrument Use of CytoBond Sealant is required for sealing coverslips to prevent probe evaporation without humidification Contact SciGene custserv scigene com or your local distributor to order additional bottles Environmental Requirements Ensure that the area where the CytoBrite System is installed meets the following conditions for reasons of safety and performance 15 to 32 C 58 to 90 F Relative humidity 20 to 80 RH non condensing Air flow clearance 6 inches 15 cm minimum on both sides Installing the Instrument Place the instrument on a level surface within a few feet of the power source Position the system so the lid can be easily opened without interference Allow 18 inches of vertical clearance and 16 inches of horizontal clearance Ensure 3 inches of clearance at the back to attach the power cord Using only the power cord provided plug it into the back of the unit and then to a properly grounded outlet Turn on power using the ON OFF switch on the front control panel Allow 18 inches of vertical clearance to open the lid Allow 3 inches of clearance at the back for the power cord clearance for air flow Version 1 1C SW 2 0 August 2015 7 408 733
16. eens enter the slide incubation temperature and time for the probe hybridization step CREATE FISH101A Protocol End Off 5 Complete creation of the protocol by choosing how to end the protocol Selecting Off turns off the slide block Selecting Hold keeps the slides at a selected temperature until removed Hold CREATE FISH101A If Hold is selected as the protocol end option enter a Hold temperature 37C Hold Temp Save the completed protocol to the controller memory or a USB key Make sure a USB key is inserted BEFORE selecting the USB option Pe FISH101A Save in CytoB rite USB Return to Home screen Version 1 1C SW 2 0 August 2015 10 408 733 7337 techserv scigene com SciGene B Editing a Protocol CytoBrite Slide Incubation System USER MANUAL The Edit function allows changes to the Denature and Incubate times and temperatures as well as protocol end options Off or Hold in an existing protocol Edited protocols may be saved under the existing name or be renamed using the Save As function to the controller memory or USB key Select Edit from the Home screen Choose the file location controller memory or USB key and the protocol to edit Select Denature Incubate or End step to edit Make changes to time hours minutes seconds and or temperature 15 90 C for the selected step Confirm changes then select Finish and Save or Continue Edits Sa
17. ertissement Pour le maintien de la Protection contre l incendie remplacer uniquement avec la m me cote de Type de fusible Toujours d brancher le cordon d alimentation avant d essayer de remplacer le fusible Version 1 1C SW 2 0 August 2015 4 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL E Cautions Failure to comply with the following cautionary statement affixed to the product may lead to possible personal injury Omission de se conformer la mise en garde suivante appos e sur le produit peut entrainer des blessures possibles This symbol located both on and in front of the lid indicates the potential presence of a Hot Surface Use care when working in this area to avoid being burned Ce symbole situ sur et devant le couvercle indique la pr sence possible d une Surface chaude Faire preuve de diligence lorsqu il travaille dans ce domaine pour viter d tre br l This symbol located on the back of the instrument warns the user to select the A correct voltage before connecting the power cord Operating with the voltage selector switch in the wrong position will damage the instrument and create a safety hazard Ce symbole situ l arri re de l appareil avertit l utilisateur de s lectionner la tension correcte avant de brancher le cordon d alimentation Fonctionnant avec le s lecteur de tension dans la mauvaise position peut endommager l appareil et cr
18. for a typical protocol is shown below fsetto TES DT OFF 4 Upgrade SW v ON E CytoBrite_Datalog_Example Notepad SETTINGS SETTINGS Datalogging Off Datalogging On Tum On Tum Off Yes Change File Edit Format View Help Datalog for Protocol TEST CYT Protocol Summary Preheat 37c OOhrs 15mins O00s5ecs Denature 75C OOhrs 10mins O0secs Incubate 37c OOhrs 10mins O0secs Enter Datalog Frequency Hold Temperature 37C 5 sec 60 min Change 00 min 05 sec Time at Step Set Temp Actual Temp 00 00 37C 21 AC 00 01 00 37C 00 02 37C Hau 37C 00 37C 37C FFC 37C 37C 37C 37C 37C 37C 37C 37C 37C 37C 37C ZC 50 rC ZAC ZAC 75C TC ZAC ZAC ZAC ZAC d 37C 37C 37C 37C 37C 37C 37C 37C 37C 37C 37C 37C 8 Datalog Frequency 00 min 05 sec Ok Yes Return to Settings Preheat Denature Denature Denature Denature Denature Denature Denature Denature Denature Denature Denature Denature Incubate Incubate Incubate Incubate Incubate Incubate Incubate Incubate Incubate Incubate Incubate Incubate END PROTOCOL RRRRRRRRRRNRRRRRRRRRRRRARARAA Lu d KE Aare Co A oo 00 00 DU 00 oO 00 DU 00 oo 00 DU DU 00 00 DO DU DU 00 00 4 00 4 DU 00 oo 00 009 4 DU OO 3 OO 00 DU 00 3 00 3 OO DO DU 00 3 OO 00 ssssssssssssssssssssssssssssssssssss Datalog file for a typical protocol Version 1
19. gust 2015 Electrical equipment for measurement control and laboratory use to include Class A Safety requirements for measurement control and laboratory use 3rd Edition Part 1 General requirements Sunnyvale California USA Place of Issue March 19 2015 Date of Issue 21 408 733 7337 techserv scigene com
20. instrument should only be used according to instructions provided in this User Manual and other SciGene technical documents If the equipment is used ina manner not specified by the manufacturer the protection provided by the equipment may be impaired La CytoBrite syst me est destin pour le chauffage et le refroidissement d chantillons biologiques L instrument ne devrait servir que selon les instructions fournies dans ce manuel de l utilisateur et d autres documents techniques de SciGene Si l quipement est utilis de mani re non sp cifi e par le fabricant la protection assur e par l quipement peut tre compromise Instrument Safety Before operating the instrument read the information in this section concerning hazards and potential hazards Ensure that anyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument Avant le fonctionnement de l instrument lisez les renseignements dans cette section concernant les risques et les dangers potentiels S assurer que toute personne impliqu e avec le fonctionnement de l instrument est instruit dans les pratiques g n rales de s curit pour les laboratoires et les pratiques de s curit sp cifiques pour l instrument Avant le fonctionnement de l instrument lisez les renseignements dans cette section concernant les risques et les dangers potentiels S assurer que toute personne impl
21. iqu e avec le fonctionnement de l instrument est instruit dans les pratiques g n rales de s curit pour les laboratoires et les pratiques de s curit sp cifiques pour l instrument Symbols and Conventions The following chart is an illustrated glossary of the electrical symbols used on the CytoBrite System Whenever such symbols appear on instruments please observe appropriate safety measures Le tableau suivant est un glossaire illustr des symboles lectriques qui sont utilis es sur le systeme Chaque fois que ces symboles apparaissent sur les instruments veuillez observer les mesures de s curit appropri es 1 Electrical Symbols This symbol indicates that this is a protected ground terminal that must be connected to earth ground before any other electrical connections are made to the instrument Ce symbole indique qu il s agit d un terminal de terrain prot g qui doit tre connect la terre avant que toutes les autres connexions lectriques sont apport es l instrument Version 1 1C SW 2 0 August 2015 3 408 733 7337 techserv scigene com SciGene A CytoBrite Slide Incubation System USER MANUAL CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution ATTENTION Ce symbole vous avertit consulter ce guide de l utilisateur pour plus d informations et de proc der avec prudence This symbol indicates the OFF position of the m
22. nsesasacemeansscnensusbasonisencseusmmmessusenensenvenanees 12 Vi RUNNINGAPROTOCOE na curecuysuei ace nessanaoae nas astro te tan ce ct ti ce 13 VI MANAGING FILES een end aa de ae na a a nn 14 As CVI APO OC de dattes onde de ten do dan an te ne a na ue 14 Be MM OVI Wee ee ee A E 14 C Ke titel e e EE 14 D Deleng 4 PFOOCOI 2 Ra a tee deus dcedaptuseauetaeiaasrenben aaa usmeanostoonsecetees 14 WIN SETTINGS AND PREFERENCES 52e csneenceerauennsensrscenesa E EE 15 A Datalogging to USB Key cccccscccccccssssscceccseseccccseeeceecsaaececeeesaecceesseusecesseaeeeeesssassecessaeaseeeasecessanasseessages 15 Be Calibrating Slide Block T MOBTA EURE scene mraces ann uns a ones cons nea nee cae es rente ner area 16 C STAGE SOLU W E 17 D FTV ie AMEL SOUS ee oan teed sane nay de E a teens 17 DC MAINTENANCE sense snnecosae none nan ecnaneneanenentaer tele naa nana sens N 18 As Cleaning the 1S EF VC NE eege Eege 18 Cleaning Alr EE 18 Checking and Replacing Fuses sise seen 18 Me ef E eier ln LCE 19 Mle SPECIFICATIONS nn ae een aa ee ete er er ann a es tie 19 Xi ORDERING INFORMATION EE 20 XIII DECLARATION OF CONFORMITY eieiei ergeet e ie e Ee 21 Version 1 1C SW 2 0 August 2015 2 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL I SAFETY NOTICES A Intended Use The CytoBrite Slide Incubation System is intended for the heating and cooling of biological samples The
23. ntroller memory or USB key FILES File 37to4 Enter new name 37to65 FILES File 37to4 Copy to USB Key 3 Select Name Enter a new name by choosing characters space A Z 0 9 using mee JLE Copy 37to4 to File 37to65 the Forward button USB key Finish and save Sequentially select a desired Yes Yes character using the Select button Protocol names must be 8 characters in length including spaces Return to Home screen 5 Select Yes to save D Deleting a Protocol 1 Select Files from the Home screen 2 Choose the file location controller memory or USB key and protocol 3 Choose Delete and press Select 4 Select Yes to confirm Copy Rename Delete a Protocol Delete FILES Delete 37to4 Yes No 408 733 7337 techserv scigene com D SC G e n e CytoBrite Slide Incubation System USER MANUAL VIII SETTINGS AND PREFERENCES A Datalogging to USB Key A text file that captures the block time and temperature while a protocol is running can be created and written to the USB key 1 From the Home screen go to Settings Datalog Creating a Datalog 2 Following the prompts on the screen turn on datalogging and enter the data sampling frequency 5 sec to 60 min Run ae 3 Insert a USB key into the USB DRIVE to capture a log file Figs SSC While in use the drive light will flash as data is transferred to the USB key SETTINGS 1 Datalog A datalog file
24. plug the power cord before performing any cleaning procedure Tournez le commutateur d alimentation sur la position OFF et d branchez le cordon d alimentation avant d effectuer toute op ration de nettoyage Remove liquid or condensation wipe dry while instrument is OFF and block is cool or at room temperature Enlever le liquide ou la condensation essuyer tandis que l instrument est teint et le bloc est frais ou temp rature ambiante B Cleaning Air Vents Clean the air intake bottom of instrument to prevent vents from becoming clogged Remove any dust and debris airflow may be hampered effecting performance of the instrument and damage by overheating of components C Checking and Replacing Fuses There are two fuses located in a removable fuse block below the power cord receptacle on the back of the unit A blown fuse appears dark Always replace fuses with those of the Same amperage and voltage as shown on the label below the fuse block Turn the power switch to the OFF position and unplug the power cord before performing any service procedure To remove fuses J Pry open tab 1 Insert a small flat blade screwdriver into the A with screwdriver tab recess just below the plug receptacle AN if if 2 Push down to release the fuse block uff Fuse holder 3 Slide fuse holder out from the fuse block while ae SC Push clip up holding the retaining tab out of the way oon Planes 4 Gentl
25. ring on the instrument during boot up New software versions are installed via the USB DRIVE Current software versions may be downloaded from www scigene com 1 2 From the Home screen go to Settings Upgrade SW Following the on screen prompts insert a USB key containing new software into the USB DRIVE and press Select A flashing progress indicator displays as the software is loading This process may take several minutes When loading is finished an Upgrade Complete message will display Press any key to display the new software version number and date After a few seconds the Home screen will appear The instrument is now ready for use D Changing Alert Sounds The CytoBrite System comes from the factory with alert sounds set to OFF Alert sounds can be turned ON to notify the user of the following events Protocol End two long beeps at protocol completion Each Select one short beep each time Select button is pressed Preheat continuously beeps upon reaching set temperature until Select button is pressed Denature one short beep indicates this step has completed To turn sounds on or off 1 2 Version 1 1C SW 2 0 August 2015 17 From the Home screen go to Settings Sounds Use the Forward button to choose an alert from the list and press Select Use Forward to choose ON or OFF and press Select Continue to change alert settings as in step 2 above or exit to the Home
26. ve edited file to memory or USB key To rename file select Save As and create new name before saving EDIT File100 Denature Yes Yes EDIT File100 Denature Temp 73 Time 00 02 00 Set Temp and Time Continue Edits EDIT File100 Temp Time OK Yes Yes EDIT File100 Finish and Save Continue Edits Finish EDIT File100 Save As Save Save As EDIT EDIT File100 Save in CytoB rite File101 CytoBrite EDIT File100 Save in CytoBrite Yes No Return to Home screen Version 1 1C SW 2 0 August 2015 11 Enter Name Denature EDIT File100 Denature Off Yes Editing a Protocol CytoBrite Run Create View Edit Files Settings EDIT File Location CytoB rite EDIT Select File 1 FILE100 2 FILE200 EDIT File100 Denature ON Incubate End Incubate End EDIT File100 End EDIT File100 Incubate Temp 37 Time 14 00 00 No Hold EDIT File100 Temp Time OK EDIT File100 Hold Temp 37 Select New Temp 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL V ONETEMP MODE The CytoBrite System can be operated to maintain a single block Running OneTemp Mode temperature from 15 to 90 C using the OneTemp mode For information on creating and saving multi temperature and multi step protocols see section V CREATING AND
27. w protocols to run directly from a USB key e Provide additional capacity for storing protocols beyond the 10 file storage limit on the controller e Upload new controller software USB DRIVE Version 1 1C SW 2 0 August 2015 9 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL IV CREATING AND EDITING PROTOCOLS A Creating a Protocol Creating a Protocol Protocols are created and saved in memory on the controller Satis 10 files maximum or a USB key Names are 8 alphanumeric i sh characters in length including spaces For FISH ISH methods An protocols can be created with an optional co denaturation step prior to probe hybridization Enter Name FISH101A 1 From the Home screen select Create 2 Enter the name of the protocol by scrolling through the alohanumeric characters space A Z 0 9 using the Forward button Sequentially select a desired character using the Select button Names must be 8 characters in length including spaces CREATE FISH101A Denature Yes Yes No CREATE FISH101A Denature Temp 73C CREATE FISH101A 3 Onthe next screen select Yes to use a se Denature step If chosen enter the Denature time hours minutes seconds and CREATE CREATE temperature 15 90 C on succeeding screens FISH101A FISHIOTA Denature Time Incubate Time 00 02 00 16 00 00 4 Onthe following scr
28. y pry out the fuses hen s Fuse block Version 1 1C SW 2 0 August 2015 18 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL X TROUBLESHOOTING Instrument does not turn on Blown fuse s Replace Er on back of unit beneath power cord receptacle Block is not reaching set Paper or other Check underneath CytoBrite base for temperature object blocking fans objects blocking the fans Ensure proper clearance XI SPECIFICATIONS Cat 2019 00 1 115 230V AC 50 60 Hz 6A 3A Dimensions cover closed Outside HxWxD 9 25x9 x 12 75 inches 24 x 23 x 32 cm 1 os 7 Ke ne Performance ECC Environmental Ambient temperature Operation 15 to 32 C 58 to 90 F Storage 20 to 60 C 4 to 140 F Relative humidity Operation 20 to 80 RH non condensing Storage 10 to 90 RH non condensing Version 1 1C SW 2 0 August 2015 19 408 733 7337 techserv scigene com S C G e n e CytoBrite Slide Incubation System USER MANUAL XII ORDERING INFORMATION 2019 70 1 CytoBrite Slide Oven 115V Includes ten 6 slide racks 2019 20 1 CytoBrite Duo Slide Incubation System 115 230V Includes two 6 slide racks 2019 10 0 CytoBrite Slide tray Holds 1 to 6 slides m m gt m m gt A m m gt gt 2010 00 1 FISH Wash Buffer 1 0 4xSSC 0 3 IGEPAL pH 7 gt 2010 00 2 FISH Wash Buffer 2 2xSSC 0 1 IGEPAL pH 7 m gt
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