H201-K-Frame User Manual
Contents
1. www oko lab com be H201 K FRAME User Manual March 2015 2 Lab Tek II 1in x2in chambered cover glass 2 60mm Petri dish 4 35mm Petri dish 1 1x3in chamber slide and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 2 35mm Petri dish 1 Lab Tek II 1in x2in chambered cover glass and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 1 60mm Petri dish 1 Lab Tek II 1in x2in chambered cover glass and 1 60mm Petri dish 1 1x3in chamber slide and 1 60mm Petri dish 1 60mm Petri dish and 1 35mm Petri dish Magnetic lock to hold the standard MW plates in the chamber Magnetic lock to hold the low profile MW plates in the chamber r gt gt GS35 M 4x35 M 2x60 M GS60 M 1x60 M LABTEK 60 M For 1 1 x3 chamber slide and For 4 35 mm For 2 60 mm For 1 60mm petri dish and For 1 60 mm For 1 Lab Tek 1 x2 chambered 2 35mm petri dishes petri dishes petri dishes 1 1 x3 chamber slide petri dish cover glass and 1 60mm petri dish 1x35 M 6035 M 1xGS M 2xGS M 1xGS 2xLABTEK M 1xLABTEK M For 1 35 mm For 2 35 mm petri dishes and For 1 1 x3 For 2 1 x3 For 1 1 x3 chamber slide For 1 Lab Tek petri dish 1 60mm petri dish chamber slide chamber slides and 2 Lab Tek 1 x2 1 x2 chambered chambered cover glass gt glass LABTEK 35 M LABTEK 60 M 1xLABTEK M 2xLABTEK M LABTEK II 35 M 2xLABTEK II M For 1 Lab Tek 1 x2 For 1 Lab Tek 1 x22. For 1 Lab Tek 1 x2 For 2 Lab Tek For 1 Lab Tek Il For 2 Lab Tek Chambered cover glass Chambered cover glass Chambered cover glass 1 x2 chambered 1 x2 chambered cover glass 1 x2 chambered cover glass and 2 35mm petri dishes and 1 60mm petri dish cover glass and 2 35mm petri dishes Figure 2 Available sample holders The following heated glass Lids are available for H201 K FRAME H201 SLIDING LID For easy sample loading and pipetting Included in code H201 K FRAME oc H201 KOEHLER LID Reduces chamber height to 21 mm and allows imaging under Koehler illumination H201 INJECTION LID Glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample Compatible only with sample holder 1x35 H201 LASER INTERLOCK LID Glass lid with safety switch Connects to laser controller and automatically turns laser off when lid is lifted Compatible only with sample holder 1x35 OPTIONAL not included with H201 K FRAME www oko lab com Page H201 K FRAME User Manual March 2015 3 1 Sliding lid The Sliding Lid is a glass lid allowing for easy sample loading and pipetting The Sliding Lid is screwed onto the cham ber Figure 3 shows chamber dimensions with the Sliding Lid with and without chamber riser Figure 4 illustrates how to remove Sliding Lid when a different Lid is necessary NOTE Sliding Lid MUST BE REMOVED when using any other Lid The Sliding Lid is f
3. Working with Pel dE Te e DE 13 11 Connection of the Chamber with XY stage ssesescesesoecesoecesoecesoecesoecesoecesoecesoeceseecesoeceseeceseeceo 14 es www oko lab com H201 K FRAME User Manual March 2015 1 Components and dimensions H201 K FRAME includes the following components Chamber main body Sliding glass lid for easy pipetting Chamber riser It is a removable frame increasing the height of the chamber from 24 to 30 mm often re quired when using multi well MW plates Sliding Lid 3 Distance between focal plane and upper side of the glass lid with the chamber riser 24 Distance between focal plane and upper side of the glass gt lid without the chamber riser PE d Sample Holder Order Separately Chamber Riser F Chamber top view Chamber Base Figure 1 H201 K FRAME Components and Dimensions 109 70 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish 1 Nunc and Greiner T25 flask 2 35mm Petri dish 2 1x3in chamber slides 2 Lab Tek 1in x2in chambered cover glass
4. 015 2 2 BD Falcon 35x10 Willco 35x10 Figure 12 Ring selection scheme for 35 mm dish Se Grainer Petri35x10 e Ibidi p Dish 35 mm low e Corning 35x10 mm e Corning 60x15 mm www oko lab com MatTek P35G X14 X eS Ibidi p Dish 50 mm low MatTek P50G X30 X CO BD Falcon 60x10 Figure 13 Ring selection scheme for 60 mm dish CG Willco 60x15 Grainer Petri 60x15 ch page LO H201 K FRAME User Manual March 2015 7 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 1 www oko lab com H201 K FRAME User Manual March 2015 8 Working with MW Plates Magnetic locks and Chamber ris er Magnetic locks hold MW plates in place into chamber illustrated in Figure 16 NOTE Magnetic locks must be ordered separately NOT INCLUDED Product codes MW LOCK 22 5 and MW LOCK 16 5 Magnetic lock 22 5 SE EE MW 22 5 LOCK To be ordered separately Typically used for 6 12 24 48 MW Figure 16 Magnetic locks for MW plates To be ordered separately ployed Figure 17 shows how to mount and screw the chamber riser Sc
5. H201 K FRAME User Manual March 2015 H201 K FRAME Compatible with the following XY stages o ASI all models including z piezo Leica all models with 160x110 opening Ludl all models with 160x110 opening Marzhauser all models Olympus all models with 160x110 opening Prior H107 Zeiss all models with 160x110 opening www oko lab com Compatible with the following Okolab Controllers H201 T UNIT BL Page 1 H201 K FRAME User Manual March 2015 Index Me Component and CNS SIONS EE 3 L Same RTE ga ere Pee ee ane EP ene E Pee tT eT EE 3 2 1 Available Sample e 3 SB MVAUADIC Lids sssssyiriseri rrira S seca sear eecanaarsccaeaas ess 4 Li SANS UC ME 5 Cy ae Ee Bo eee ere er Mere rare er en ORT Ore ve ee eee ee er ee err tren 6 Io MSC CIO KI aetitte acs vee ate vette vse ters setae da EE eta then seta bins eenseens eens 6 sa Eiser INGCTIOC EE 7 4 Insertion of the Sample Feedback Temperature Sensor EEN EE EEN EE EEN 8 5 Insertion of Sample Holder into Chamber sesesessesesececesesesescecececececesesescoesecececesesescecesesececeseseseoo 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS ee EEN 9 7 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS cc cce ccc ceecccceccccncccceccceecccescccesces 11 8 Working with MW Plates Magnetic locks and Chamber riser cccccccccccccccccccccecccecccecccecccecccecees 12 8 Connection OF the Gas SIE 13 10
6. ction of the springs until it snaps into the housing See Image 2 of Figure 20 3 Image 3 of Figure 20 shows the chamber fitted on the XY Stage Figure 20 Connection of the Chamber with XY Stage www oko lab com page 14
7. hows chamber dimensions with the Injection Lid with and without chamber riser s www oko lab com H201 K FRAME User Manual March 2015 28 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 22 and upper side of the glass lid without the chamber riser Chamber top view 109 70 Figure 6 H201 K FRAME H201 INJECTION LID 3 4 Laser Interlock Lid The Laser Interlock Lid is a glass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid with and without chamber riser H201 LASER INTERLOCK LID 29 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 23 and upper side of the glass lid without the chamber riser 0 Chamber top view nm 09 70 Figure 7 H201 K FRAME H201 LASER INTERLOCK LID www oko lab com ew H201 K FRAME User Manual March 2015 4 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201 K FRAME see Figure 8 Frontal and 3D views Temperature Sensor dedicated opening Temperature Sens
8. ixed onto the chamber with 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane and upper side of the glass lid without the chamber riser Chamber top view lt gt f SS Figure 4 Assembly of the sliding lid www oko lab com pee H201 K FRAME User Manual March 2015 3 2 Koehler Lid The Koehler Lid is a glass lid reducing the chamber s height to 21 mm and allowing imaging under Koehler illumination The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 5 shows chamber dimensions with Koehler lid with and without chamber riser H201 KOHLER LID 27 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 21 and upper side of the glass lid without the chamber riser Chamber top view 109 70 Figure 5 H201 K FRAME H201 KOEHLER LID 3 3 Injection Lid The Injection Lid is a glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample NOTE Compatible with sample holder 1x35 ONLY The Injection Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 6 s
9. or dedicated opening 1 Frontal view 2 3D view Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 9 Figure 9 Introduction of the Specimen Holder inside the Chamber Base www oko lab com pase H201 K FRAME User Manual March 2015 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Figure 10 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 11 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figure 13 respectively Figure 11 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com sec H201 K FRAME User Manual March 2
10. rews location is indicated by letter A Chamber Riser Figure 17 Riser Assembly www oko lab com Chamber Riser increases chamber s height and is REQUIRED with 6 12 24 48 MW plates regardless of the Lid em el 2 H201 K FRAME User Manual March 2015 9 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H201 K FRAME Silicon tubing connects to a gas input brass opening located on a corner of the H201 K FRAME See Figure 18 Connect by gently pushing silicon tubing onto brass opening Gas Input Figure 18 Connection with gas supply 10 Working with Perfusion The Chamber Riser included with H201 K FRAME features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as necessary before introducing perfusion tubing Figure 19 shows location of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 19 Perfusion www oko lab com bape 3 H201 K FRAME User Manual March 2015 11 Connection of the Chamber with XY stage H201 K FRAME fits on the XY Stage without any screws To fix the chamber on the stage follow the steps shown in Figure 20 1 Place the chamber on the stage See Image 1 of Figure 20 2 Incline the chamber and slide it in dire
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