SC-200 Manual
Contents
1. 0 5 V portion of pH level is shown 1 to 14 Next three decimal places are shown 1 11 Readings above 14 000 are shown as Readings below 0 000 are shown as SC 200 manual 6 Version 1 0b ze Section Enulpment Required 4 pH pH probe or precision The raw voltage output from the Voltage voltage source Do instrument s pH amplifier is displayed as not exceed 0 5 V X XX volts Reading can range from 0 00 m e 7 Battery Install two AA The battery voltage is displayed as X XX Voltage batteries volts 8 Character None Every ASCII character space to is Set displayed Due to the limitations of the 7 segment format some characters are not used by the software 9 Number None The display cycles through showing Display every possible digit and every decimal point 10 Sound The beeper is turned on continuously Test SC 200 manual 7 Version 1 0b2. Test Mode is organized into sections Press the POWER button briefly to move to the next section After the last section Test Mode restarts the first WARNING DO NOT MOVE THROUGH SECTIONS WITH THE pH PROBE ATTACHED This can damage the electrode Only connect the pH electrode if needed in section 2 or 4 Always remove the pH electrode before leaving these sections Combinations of the yellow MODE LEDs are illuminated to indicate the section number as shown in the table below The Stop LED red is illuminated when an error is detected by the instrument The Proceed LED green is illuminated to indicate no error detected The green LED does not guarantee proper functioning it only indicates that no problem could be automatically detected The user should make careful observations to discern proper operation To exit Test Mode hold the POWER button down 5 10 seconds until the instrument shuts off If the device does not shut off after 10 seconds of holding down the button move to the next section by releasing then pressing again the POWER button briefly then try to exit again oe Equipment Required Description 0 Version None The version l of the instrument software is displayed The instrument goes through a 1 Burn in None continuous burn in cycle of its sound LEDs and display pH probe or precision An uncalibrated pH level is shown in two voltage source Do alternating parts First the integer not exceed
3. CAL LED flashes to convey that calibration for this value is ready Press the ENTER button to accept the calibration The display stops flashing scrolling the message Good Cal and four beeps are rapidly sounded to indicate success Note if an error occurs during this process the message Bad Cal will scroll the instrument will then continue to wait for a stable pH level Repeat step 4 Now rinse the electrode again and place it in the second member of the calibration set e g pH 7 00 Repeat the process to get a second Good Cal message Exit into pH or TA mode Measuring pH 1 Make sure the pH electrode is attached Calibrate it as described above if necessary Select pH mode with the MODE button 2 Rinse the pH electrode with DI water Gently shake off or carefully blot away excess liquid 3 Place the electrode in the solution to be tested Be careful not to let the electrode strike any surfaces 4 Allow the pH reading to stabilize Typically this takes about 10 15 seconds Read the pH value on the display Titratable Acidity TA l If you are working with a sample of must we recommend homogenizing your sample in a blender before proceeding otherwise your TA values can be very inaccurate Take 100 mL or more of your must and put it in a blender on high for 30 seconds Allow solids to settle for 2 minutes before sampling Fill the syringe by drawing up the TA Titrant 0 13N NaOH Expel bubbles and set the
4. glass bulb nor attempt to wipe it with anything When necessary you may gently blot excess liquid away from the electrode surface but avoid directly touching it The pH electrode should always be kept in the liquid storage bottle with its special solution available from Vinmetrica when not in use Rinse the electrode with DI water and gently blot or shake off excess water Push the electrode through the hole in the cap about an inch then gently screw the bottle onto the cap so that the electrode is in contact with the solution in the bottle OPERATION Turn on the instrument by pressing the POWER button briefly Note depressing the POWER button longer than two seconds at start up will cause the instrument to enter Test Mode see Appendix The instrument will go through a power up sequence After a few seconds the instrument will start in whatever mode was last selected The mode is indicated by the yellow LED panel on the left Select the desired mode by pressing the MODE button 1 pH mode In this mode the meter measures the pH The pH electrode must be attached If the instrument has not yet been calibrated the message doCal scrolls across the screen and you need to do a calibration see below We recommend re calibrating the instrument for pH once each day of use 2 TA mode This is for titration in determining titratable acidity As in the pH mode the pH is displayed and the pH electrode must be attached The green Procee
5. plunger on the syringe to a readable point preferably the 5 0 mL point Note the 5 0 mL SC 200 manual 3 Version 1 0b setting allows determination of up to 10 g L TA ina standard 5 mL wine sample If you are using a buret instead fill it now Caution the TA Titrant 0 13N NaOH is caustic and can cause damage to clothing skin and eyes We recommend use of laboratory safety glasses and latex or nitrile gloves during this procedure If any solutions contact skin or eyes flush with plenty of water 3 Place 5 0 mL wine or must in the titration vessel 100 mL polypropylene beaker We recommend using the 5 mL pipette provided in the kit draw sample up to the 0 mL mark then dispense the sample into your titration vessel by letting the tip of the pipette touch the side of the vessel while the sample drains For best accuracy do not blow out the liquid that remains in the tip Add about 15 ml of DI water 4 Turn on the instrument Make sure the pH electrode is attached If necessary calibrate it as described above Select TA mode with the MODE button 5 Ifyou are using a magnetic stirrer turn it on to stir at a moderate rate Be sure the stir bar will not strike the electrode in the next step 6 Rinse the electrode briefly with distilled water Insert the electrode into the titration vessel so that the tip is completely submerged to just above the circulation gaps cutouts at the tip of the electrode 7 Ifyou are stirring manually begi
6. MAXIMUM LIABILITY FOR ALL DIRECT DAMAGES INCLUDING WITHOUT LIMITATION CONTRACT DAMAGES AND DAMAGES FOR INJURIES TO PERSONS OR PROPERTY WHETHER ARISING FROM VINMETRICA S BREACH OF THESE TERMS AND CONDITIONS BREACH OF WARRANTY NEGLIGENCE STRICT LIABILITY OR OTHER TORT WITH RESPECT TO THE MATERIALS OR ANY SERVICES IN CONNECTION WITH THE MATERIALS IS LIMITED TO AN AMOUNT NOT TO EXCEED THE PRICE OF THE MATERIALS IN NO EVENT SHALL VINMETRICA BE LIABLE TO BUYER FOR ANY INCIDENTAL CONSEQUENTIAL OR SPECIAL DAMAGES INCLUDING WITHOUT LIMITATION LOST REVENUES AND PROFITS HAZARDS AND TOXICITY All Materials offered by Vinmetrica are intended for use by individuals who are familiar with laboratory procedures and their potential hazards The Materials contain chemicals which may be harmful if misused Due care should be exercised with all Materials to prevent direct human contact Keep out of reach of children Viumetuca 1945 Camino Vida Roble Suite E Carlsbad CA 92008 www vinmetrica com 760 494 0597 info vinmetrica com Copyright 2012 Sportsman Consulting LLC DBA Vinmetrica All rights reserved SC 200 manual 5 Version 1 0b Appendix Test Mode Test Mode provides various special functions that may be useful in testing the device for example if troubleshooting is necessary Test Mode is entered when the POWER button is pressed longer than 2 seconds while turning the instrument on Remove the pH electrode if it is attached
7. a t Vinmetrica SC 200 Vinmetuca User Manual The Vinmetrica SC 200 is a simple and robust device that provides high accuracy in determination of pH and titratable acidity TA levels in wine These are essential parameters to control in the effort to make high quality wines Theory of operation The SC 200 kit provides a pH electrode and reagents for calibration and determination of pH and titratable acidity TA values in wines and other samples The pH value is simply determined by placing the calibrated electrode into a sample and reading the value TA is determined by titrating a 5 mL sample of wine to an endpoint pH of 8 2 with the TA titrant 0 13N NaOH from the syringe in the kit From the known concentration of the TA titrant and its volume required to reach the endpoint the TA is simply calculated results are in units of g L tartaric acid Materials provided in the kit 1 Vinmetrica SC 200 pH controller unit Part number SC200 1 2 pH Electrode Part number SC200 3 red polycarbonate housing with storage bottle and storage solution 3 pH TA reagent set Part number SC200 2 pH 4 01 calibration solution pH 7 00 calibration solution TA titrant 0 133N NaOH 4 One 5 mL syringe 5 One 5mL serological pipette 6 100 mL polypropylene beaker Things you will need 1 Two standard AA batteries alkaline type 2 Distilled water it s handy to have a wash bottle or similar for rinsing 3 Optional magnetic sti
8. culation is simply TA 2 V_ i e 2 times V SC 200 manual 4 Version 1 0b Finish up 1 Turn off the instrument 2 Rinse the syringe with distilled water Let air dry 3 Be sure to rinse and store the pH electrode as directed on page 2 4 Store all reagents tightly capped and away from heat and sunlight Technical assistance info vinmetrica com _ tel 760 494 0597 WARRANTIES AND LIABILITIES 1 The materials provided in the kit as described on page 1 above Materials are warranted as follows The SC 200 instrument electrode and non reagent accessories are warranted against defects in workmanship for 12 months from date of purchase The reagents are warranted to perform as described herein up until any stated expiration date or 6 months after purchase whichever is later THE WARRANTIES IN THESE TERMS AND CONDITIONS ARE IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MERCHANTABILITY NONINFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE SAID WARRANTIES BEING EXPRESSLY DISCLAIMED 2 Buyer agrees that its sole and exclusive remedy against Vinmetrica shall be limited to the repair and replacement of Materials or parts of Materials provided Vinmetrica is promptly notified in writing prior to the expiration of the warranty period specified above of any defect Vinmetrica s liability for any damages due Buyer shall be limited to the purchase price of the Materials 3 VINMETRICA S
9. d LED is lit if the pH is below 8 2 while the red STOP LED is lit if the pH is 8 2 or greater see below under Procedures Titratable Acidity 3 CAL mode This is for calibrating the pH electrode which must be attached The display initially shows CAL for a few seconds as it prepares to read pH and lets readings settle Thereafter the display shows the measured pH level with two decimal places Calibration works with one of the following calibration sets SC 200 manual 2 Version 1 0b pH 4 01 and 7 00 or 4 7 pH 7 00 and 10 00 or 7 10 pH 3 00 and 7 00 or 3 7 Vinmetrica recommends use of the 4 7 set provided with the kit Procedures Calibration of pH l Be sure the pH electrode is attached to the unit then select CAL mode by pressing the MODE button until the CAL LED illuminates Choose a calibration set that corresponds to the range you are working in Usually for wine this will be at pH values below 4 so use the 4 7 set If you have a source of a pH 3 00 reference solution you can use this in place of pH 4 Rinse the electrode with DI water shake off excess liquid gently and place the electrode into a small vessel containing either member of one calibration set e g pH 4 01 The instrument will determine which calibration solution is being used and will display the apparent pH value When the pH level is sensed as stable the nominal value is shown on the display flashing and the
10. n now use a moderate swirling motion If the electrode 1s not held in a stand hold it against the side of the vessel with one finger and grasp the vessel with the remaining fingers so that the two move together Figure 2 8 Verify that the pH 1s less than 7 and the green PROCEED LED 1s lit If the pH 1s greater than this and or the red STOP LED is lit and the beeper sounds your sample has less than 0 1g L TA and there is no need to proceed 9 Titrate the sample by adding the TA Titrant dropwise from the syringe or buret being sure to note the starting volume mark on the syringe or buret During the titration the pH will gradually rise from its starting value below 4 usually As you approach pH 7 go slowly in adding successive drops of titrant so as not to overrun the endpoint Be sure to mix thoroughly after each successive drop of titrant Take the endpoint as the first addition of TA Titrant that causes the pH to stay above 8 2 for longer than 15 seconds The STOP LED and the beeper will provide additional indication of the endpoint Read the endpoint volume off of the syringe or buret To silence the beeper after the endpoint select pH mode or turn off the instrument 10 Calculate the TA value as V 0 133 75 TA g L tartaric S where V mL Titrant needed to reach the endpoint N normality of the Titrant S mL sample If you use 5 mL of sample as directed and the Titrant is 0 133 N as supplied then the cal
11. rrer and stir bar electrode stand burette Figure 1 The SC 200 US Patent pending SC 200 manual 1 Version 1 0b Setting up the instrument 1 The SC 200 Figure 1 runs on two standard AA batteries alkaline cells recommended To insert the batteries open the battery housing on the bottom of the back of the unit by removing the two screws and gently prying off the lid Install the batteries as shown by the and markings then close the housing If desired you can prop the unit up using its folding stand Low Battery Detection When the battery level is getting low the instrument shows a low battery icon on the upper left side of the display but continues to operate without impairment to any function Replace the batteries as soon as practicable When the battery level drops too far the instrument does not operate It rapidly flashes the low battery icon for 3 0 seconds beeps and shuts itself off Auto Shut off The SC 200 shuts off after 30 minutes If this happens unexpectedly just press the POWER button to resume from where you were 2 Attach the pH electrode via the BNC connector protruding from the top About the pH electrode The pH electrode is fragile and should always be handled carefully Remove the liquid storage bottle by unscrewing the cap FIRST then gently pulling the bottle followed by the cap off the electrode Rinse the electrode in a little distilled water before each use Electrode care Do not touch the
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