Release Notes - Bio-Rad
Contents
1. Component Minimum Recommended Operating system Windows 2000 or XP Windows XP Professional Note Workstation version of Bio Plex Manager requires Windows XP Processor Pentium IV or equivalent 2 8 GHz Pentium IV or higher 2 8 GHz or higher Hard disk space 40 GB 80 GB System memory 512 MB 1024 MB Screen resolution 1024 x 768 1280 x 1024 Screen colors 256 colors 24 bit True Color Ports for connecting instrument required 1 RS232 serial port and 1 RS232 serial port for Workstation license only 1 USB port and 1 USB port Port for connecting the HASP key 1 USB port 1 USB port Other software Internet Explorer 6 0 or later Internet Explorer 6 0 or later Microsoft Excel 2000 or later Microsoft Excel 2000 or later 4 Upgrade Kits Upgrade kits are available which contain everything needed to upgrade previous Bio Plex Manager versions including instructions See the table below for the Upgrade Kit part numbers If you are upgrading from Order Upgrade Kit Part Bio Plex Manager 4 1 or 4 1 1 171SUPG41 Bio Plex Manager 4 0 171SUPG40 Bio Plex Manager 3 0 171SUPG30 Bio Plex Manager Standard for IS 2 3 171STND23 5 Standard vs Security Edition The Standard Edition gives all users equal access to all features of the software with no restrictions and no electronic audit trail Bio Plex Manager 5 0 Security Edition provides a secure environment for the maintenance verification and tracking of all electr2. Edition Yes All of the features that were included in BPM 4 1 1 are still present in 5 0 Additionally the ability to overwrite files until they are signed has been added Is my Bio Plex system upgradeable to BPM 5 0 If your reader is running BPM 4 1 1 it is fully upgradeable to BPM 5 0 If you are running BPM 4 0 or earlier and your reader was purchased prior to October 2000 it cannot be upgraded to 5 0 If your platform was purchased prior to August 2001 it may not be upgradeable am trying to upgrade to BPM 5 0 but the LxFirmware wizard isn t detecting all of the firmware versions What s wrong The firmware wizard will only upgrade or downgrade the firmware when using the serial cables Be sure you have not prematurely connected the USB cable If the problem is detecting the HTF firmware be sure you are using the correct cable You must reconnect the platform serial cable to the HTF Be sure you are not using the CAN BUS cable that is supplied to connect the reader to the HTF upgraded to BPM 5 0 and installed the new USB cable and now the system will not connect What s wrong This could take a couple of minutes due to the automatic port detection feature If you still cannot connect after at least 3 minutes go to the Device Manager in the Control Panel and see if there is a menu item listed for Luminex Devices If not go to Actions and select scan for new hardware to see if the Luminex Devices item is cr
3. Sid Err for Obs Conc Norm Ratio and Norm FI calculations Automatic selection of DD gate and beadmap settings through a new analyte panel assay type attribute Standard MagPro Luminex MagBeads to greatly reduce user setup errors New setting in Standard Curve view allows outliers to be flagged Many new Bio Rad assay panels available through http www bio rad com bio plex x plex 2 Resources for New Users The CD contains an autorun utility to install the software database and Microsoft NET environment It also configures and starts a Windows service which enables automatic communication with the instrument when you open Bio Plex Manager Refer to the User Guide on the CD for other installation information The following documentation is available on the Bio Plex Manager 5 0 CD The documents are stored as pdf files that can be viewed with Adobe Acrobat Reader To download the Reader for free go to the Adobe Web site at www Adobe com 10010706 Page 1 of 6 Bio Plex Suspension Array System e Bio Plex Manager User Guide 10010751 e Bio Plex Manager Security Edition Configuration Setup Guide 10000528 Rev C e Hardware Protection Key Utilities to configure multiple network licenses Online help is available from the application toolbar Many other Bio Plex Manager resources are available at the Bio Rad Laboratories website http Awww bio rad com bio plex 3 System Requirements
4. files cannot be opened on BPM 4 1 1 or earlier versions Can I run Luminex MagPlex beads on the Bio Plex system Yes Go to Add Panel in the Select Analytes window of the New Protocol setup dialog and set up your analytes Next click the dropdown menu labeled Assay and select MagPlex This will set specific gate settings and bead map for the MagPlex beads 10010706 Page 6 of 6
5. Bio Plex Suspension Array System Release Notes Bio Plex Manager Software Version 5 0 October 18 2007 This document has the following sections About Bio Plex Manager 5 0 Resources for New Users System Requirements Bio Plex Manager Upgrade Kits Workstation License Desktop License Network License Reporting Software Problems and Feature Requests to Bio Rad Laboratories 10 Technical Support 11 Expanding Line of Assays Available 12 Information for Luminex Users 13 Frequently Asked Questions 2 3 4 5 Standard vs Security Edition 6 7 8 9 1 About Bio Plex Manager 5 0 Bio Plex Manager is a major revision that contains new graphing functionality an updated LXR library and many other improvements New graphing function compares results between samples and or across analytes Updated LXR library ensures compatibility with new Luminex system and xPONENT software Newly designed fluid reservoir and software support for running unattended pre or post run maintenance operations New Report Table layout for viewing Multiple Analyte sample data across analytes within a single view New Data Normalization uses internal or external controls to support gene expression analysis Users no longer need to calibrate differently for high sensitivity assays The new Run at High RP1 Target setting in the Run Protocol process now provides that functionality New statistics columns CV Std Dev and
6. ach well Why can t calibrate on high RP1 target with BPM 5 0 always used to calibrate on high RP1 target for my work and am concerned that the system will not operate optimally after calibrating on low RP1 target Although the calibration window only allows entry of the low RP1 target the CAL2 target value calibrates at low and high automatically These values are stored in the system so that if the high RP1 target is required for your run all that is needed is to check the box to Run at High RP1 Target in either the Run Protocol window or in the Validation window There is no difference from before in the accuracy of the calibration must now select the type of assay from Add Panel in the Select Analytes dialog window How do I know which type of assay I am setting up if have developed my own assay from raw beads and my own antibodies Select the type of assay based on where you obtained your beads If your beads were obtained from Bio Rad and are non magnetic you select Bio Plex or if magnetic Bio Plex Pro If your beads were obtained from Luminex or any Luminex partner select xMap for non magnetic beads and MagPlex for magnetic beads By selecting the correct assay type the software will correctly set the DD gates and bead map for your test Can my BPM 3 0 4 0 4 1 or 4 1 1 files be opened on BPM 5 0 Yes They can be opened and used Can BPM 5 0 files be opened on BPM 4 1 1 or earlier versions No BPM 5 0 generated
7. continues you have a leak in the system have purchased BPM 5 0 for my system but also have applications for which must use XxPONENT software My data is not very good after switch applications what gives Be sure to calibrate any time that you change applications as calibration settings don t carry over between the applications Run only one application at a time The same error message continually comes up during a run know this problem will not hurt my data but must baby sit the instrument to keep it running Can I turn off the message Yes If the run is in progress stop your run and go to the Utilities folder in the Bio Plex Manager 5 0 folder Double click the BWMM exe file Bio Plex Warning Message Manager Click the option to Hide the warning messages Restart your run using Rerun Recovery mode The message you were getting will no longer stop the run But beware that you have turned off ALL error messages As soon as your instrument problem is fixed be sure to return to BWMM exe and click the Show option to re enable the messages The status bar on the Desktop shows that LX Service is stopped How come am able to launch and run BPM Windows XP will show that LX Service is stopped whenever more than one user is logged onto the system Ignore it this is a windows bug that does not affect the Bio Plex system What s the purpose of Sample Timeout This allows you to set the acquisition time of the samples
8. e Bio Plex suspension array system Bio Rad Laboratories offers an expanding line of assays and reagent kits including e Cytokine assays High sensitivity cytokine assays Phosphoprotein assays Total target assays Amine coupling kit xMAP COOH beads 10010706 Page 3 of 6 Bio Plex Suspension Array System Ordering information and catalog numbers for x Plex assays in one or ten 96 well plate formats are available at www bio rad com bio plex x plex 12 Installation Instructions for Luminex Users Bio Plex Manager 5 0 is compatible with Luminex xPONENT software Because the Luminex LXR library has been updated with this version older Luminex software will not function once you upgrade to Bio Plex Manager 5 0 In order to run both Luminex and Bio Plex Manager on the same machine you must upgrade your Luminex IS 2 3 software to the current Luminex software 13 Frequently Asked Questions What operating systems does Bio Plex Manager 5 0 require The Network and Desktop licenses run under Windows XP or 2000 The Workstation license requires Windows XP to have full function Will BPM 5 0 run on my Luminex xPONENT system Yes BPM 5 0 runs with the same firmware and library as the Luminex xPONENT system Will BPM 5 0 run on my Luminex IS 2 3 system No BPM 5 0 runs with different firmware and library than the Luminex IS 2 3 system The two applications cannot be run on the same instrument Does BPM 5 0 offer the Security
9. eated If so try to connect again If you still cannot connect replace the USB cable with the serial cable that was previously installed Try connecting If the system connects you can run the system with the serial cable until field service can be notified want to reinstall the previous version of BPM to do my work because made an unsuccessful attempt to upgrade to BPM 5 0 I get Error 1722 when I begin the installation What do do 10010706 Page 4 of 6 Bio Plex Suspension Array System Remove the hasp key and do the install again but use the Custom installation method and do not install the Hasp Support portion of the installer Even though you uninstalled BPM 4 1 1 or other BPM version the uninstall process does not uninstall the hasp key The different hasp keys in the two software versions are in conflict Earlier BPM versions will operate normally using the BPM 5 0 hasp key have BPM 5 0 on my system and keep getting a message during my runs stating the sheath pressure limit was triggered during the acquisition Why The system pressure settings are not correct Go to the folder Bio Plex Manager 5 0 and open the folder Utilities Double click on the file RIPS exe In the dialog that comes up select Proceed Cycle the power on the instrument and launch BPM 5 0 The system settings will be set automatically Follow the instructions then try running samples again If the error message
10. licenses over a computer network Like the Desktop license it enables users to analyze data files but not control the array reader and platform 9 Reporting Software Problems and Feature Requests to Bio Rad Laboratories The installer for Bio Plex Manager also installs Solobug BPM in a Bug Reporter subfolder within your Bio Plex Manager folder Please use this program to request features request design changes or report non critical Bio Plex Manager problems To use Solobug click on the Windows Start button select the Bio Plex Manager folder from the Programs list and open Solobug from within this folder Enter the required information with a description of the request and select Save This creates a report for attaching to an email to Bio Rad Please email Solobug files to LSG TechServ US Bio Rad com from within the U S and LSG TECHServ IntI Bio Rad com from outside the U S 10 Technical Support Bio Rad Technical Support in the United States is open Monday Friday 6 00 a m to 5 00 p m Pacific Standard Time Worldwide technical support is available on the Web at http www consult bio rad com Phone 800 424 6723 option 2 option 3 510 741 6910 option 2 option 3 Fax 510 741 5802 E mail LSG TechServ US Bio Rad com U S LSG TECHServ ntI Bio Rad com International 11 Expanding Line of Assays Available Bio Plex assays are multiplex bead based assays based on xMAP technology and optimized for th
11. onic records generated by Bio Plex Manager These records include Protocol and Results files the Calibration Validation and Instrument Operation Logs and the Audit Trail Bio Plex Manager 5 0 Security Edition software enables compliance with FDA 21 CFR Part 11 regulations The Security Edition is controlled by a specially coded hardware protection key When the Security Mode is enabled only an authenticated user can access the software Each user is assigned to one of six user levels Each level gives the user access to specific features and functions of the software A table listing the functions accessible at each user level is provided in the User Manual on the CD included with Bio Plex Manager 5 0 10010706 Page 2 of 6 Bio Plex Suspension Array System 6 Workstation License Bio Plex Manager Workstation is designed to collect analyze and output data from the Bio Plex system It runs as a Windows application on a PC that is directly connected to the array reader and microplate platform You must run Windows XP to have full function using this license 7 Desktop License The Desktop license enables the user to analyze data files but not control the array reader and platform Instrument communication and control functions are not available with this license You can run this license on either Windows XP or Windows 2000 8 Network License The Network license allows multiple Windows XP or 2000 users to run Desktop
12. you are running to a value less than the default time Default times are based on sample volume so for a 50 ul sample volume the default sample timeout is about 80 seconds For a 100 ul volume it is about 150 seconds etc There is no reason to allow longer times because the sample will have completely run through the system by the default time But shorter times might be used to speed up the run especially if you are running a plate in which all the wells don t have the same bead regions present as might be encountered in some phosphoprotein assay runs My run is taking a long time on each sample because neglected to add one of my beads Can set Sample Timeout in the middle of a run and is it tracked Yes You can stop a run and set or change the Sample Timeout time and restart the run using Rerun Recovery The Sample Timeout time is tracked in the raw data table for each sample When would I use a 25 region map instead of the 100 region map The regions in the 25 region map are larger and will provide better classification efficiency if using Bio Rad s magnetic beads which are larger 8 um than the non magnetic beads 5 6 um 10010706 Page 5 of 6 Bio Plex Suspension Array System Can change bead region maps during a run and if do will the region used in each sample be identifiable Yes You can stop a run and change the bead map then restart the run using Rerun Recovery It will be tracked in the raw data table for e
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