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WATER MAZE VIDEO TRACKING SYSTEM

1. 43 Exporting Options Select the Format and Destination for the exported file The options are as follows Format Adobe Acrobat PDF Comma Separated Values CSV Tab Separated Text TTX Destination Application Disk File Printer Creates pdf file that can be opened in Adobe Acrobat Creates csv file that is useful when viewed in Microsoft Excel or other Spreadsheet program Creates ttx file that is useful when viewed in Microsoft Excel or other Spreadsheet program Opens the file in the appropriate application Opens a prompt to select a filename and storage location Opens a prompt to select the printer and print settings Once the Format and Destination selections have been made click OK to proceed An example report is shown in Figure 7 3 It was generated using the PDF format Figure 7 2 Export Options Format Adobe Acrobat PDF 44 Destination Description Application destination exports the report to a temporary File in the specified Format and then opens the file in the appropriate application Example Report in PDF Format The information included in the report depends on the report options enabled on the Select Trials for Reporting screen Figure 7 1 In the example below all of the options were enabled Therefore the data for Platforms Sections Thigmotaxis Moser s Zone Wishaw s Corridor and Gallagher s Proximity are all included in the r
2. 15 2 cm Features Textured surface facilitates animal climbing onto platform Figure 2 10 SG 596MR Water Maze On Demand Platform Controller Front Panel MAGNET STATUS JSSOCIJTES INE www med associates com VERMONT USA WATER MAZE ON DEMAND PLATFORM CONTROLLER SG 596MR Figure 2 11 SG 596MR Water Maze On Demand Platform Controller Back Panel MAGNET STATUS PUSHBUTTON MONITOR OUTPUT INPUT 3AG 1A eee O 250V FAST AUSA CONTROL TO PLATFORM Sn gt INPUT om CHAPTER 3 Getting Started Installing the Camera Mount It is recommended that Physical Plant complete the installation of the Camera Mount Sheet Rock Ceiling Refer to Figure 3 1 to install the camera mount on a sheet rock dry wall gypsum board ceiling Be sure that at least two of the screws are drilled into studs to ensure that the mount is secure If studs cannot be located anchors should be used Figure 3 1 Installing the Camera Mount on a Sheet Rock Ceiling A B Drop Ceiling Instructions The camera mounting system includes three clips for mounting the camera to a drop ceiling without the use of screws Orient the clips as shown Figure 3 2 and turn the clips until they are secured to the ceiling braces Once they are properly secured slide the clips into the correct location Next assemble the camera mount as shown in Figure 3 3 Slide the screws on the mounting clips through the holes in the mounting bracket secu
3. 8 14 0 Testing 120 8 14 0 Testing 120 8 14 0 Testing 120 8 14 0 Testing 120 3 14 0 Testing 120 platfor OnmDe 2 000 30 000 2 000 12 200 platfor On De 2 000 30 000 2 000 12 200 platfor On De 2 000 30 000 2 000 12 200 platfor OnmDe 2 000 30 000 2 000 12 200 platfor On De 2 000 30 000 2 000 12 200 Mi Gender 8 14 0814 lv 1 1 8 14 0 Testing 120 3 platfor OnDe 2 000 30 000 2 000 Yes 12 200 8 14 0824 iv 1 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 Yes 12 200 89 14 0834 v 1 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 12 200 ee eek ga 1 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 12 200 v 1 1 8 14 0 Testing 120 3 platfor OnDe 2 000 30 000 2 000 12 200 E 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 E 1 2 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 12 200 Strain E 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 E 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 a 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 Birth Date E 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 C 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 E 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 w 1 3 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2
4. Figure 5 20 Example Template Wizard Template Wizard Template Name Number of days Number of trials per day Total Number of Trials Start on Day Max Swim Duration sec 120 Trial type Testing Start Section Random Platform Position platform 1 Platform Type On Demand Platform Duration sec 2 000 Raise After sec 30 000 Dwell Time sec 2 000 Continuous Cumulative Figure 5 21 Example Trial Templates Screen Trial Templates felix File Edit View Template Trial Help Template Wizard Trial List Day Trial Comments Max Swim Start Section Platform N Platform T Duration Raise Afte Dwell Time DwellCont Incl 1 1 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 2 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 3 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 4 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 5 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 6 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 7 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 8 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 9 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 1 10 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 1 Te
5. The correction is made by calculating the average swimming speed for each trial then the amount of time required to swim to the platform at that speed from the start location This amount of time is subtracted from the total distance from the platform during the trial By this method scores obtained with proximity measure are designed to reflect search error they represent deviations from a direct path to the platform MED ASSOCIATES INC WATER MAZE VIDEO TRACKING CHAPTER 2 Water Maze System Hardware General Computer Environment The minimum recommended system is as follows e lt A 3 4 GHz Pentium 4 Computer or 2 33 GHz CoreDuo or higher with an available USB port and Firewire Card e Windows XP Operating System e 1GBof RAM e CD ROM Drive Keyboard and Mouse e Screen resolution of 1280 x 960 pixels recommended 1024 x 768 minimum Hardware Guide Standard Hardware Figure 2 1 ENV 598 Camera Mount amp Camera ENV 598 Camera Mount Length Adjustable from 9 5 14 0 24 1 35 6 cm med aSSOciates mo INPUT OUTPUT DIG 703A USB 100 240 VAC 50 60 Hz FUSE 3A MED ASSOCIATES INC Optional Hardware Figure 2 4 ENV 594 FI Floor Insert Figure 2 5 ENV 594 Water Maze Pool Figure 2 6 ENV 593 Pool Support WATER MAZE VIDEO TRACKING ENV 594M FI Mouse Floor Insert Dimensions 42 0 Diameter x 12 0 H 107 0 x 30 5 cm ENV 594R FI Rat Floor Insert Dimensions 66 0 Diamet
6. 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 8 14 0 Testing 120 2 latfor OnDe 2 000 30 000 2 000 Yes 12 200 34 Select the trials to run today To select a contiguous section of multiple trials click on the trial at the top of the list then hold down the shift key and click the trial at the bottom of the list To select all trials click any trial and press Ctrl A To select individual trials hold the Ctrl key and click each desired trial to highlight blue In the example shown in Figure 5 25 the first trials on Day 1 are selected Right click a blue highlighted trial or open the Trial menu and choose Modify Run Today The Run Today leftmost column will have the checkboxes filled for the selected trials After picking the trials to run today click View Main Screen and the screen shown in Figure 5 26 will appear Figure 5 25 Trials Selected E Editing Project text File Edit View Animal Trial Help Project Name Edit Pool Platforms Animal List Trial List Animal Comm MaxS Start 5 Platfor Platfor Durati Raise Dwell Dwell Includ D O 3 14 0 Testing 120 3 14 0 Testing 120 35 140 Testing 120 platfor OnDe 2 000 30 000 2 000 12 200 platfor OnmDe 2 000 30 000 2 000 12 200 plattor On De 2 000 30 000 2 000 12 200
7. 000 12 200 Weight E 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 12 200 E 1 4 2 i 1 4 2 1 9 4 E 1 9 4 E 1 4 E 1 2 E 1 2 E 1 2 Starting a Trial Select View Main Screen then highlight in blue the trial to run on the Trial List as shown in Figure 5 26 Place the animal in the pool inside the start section which is highlighted in purple Once the researcher is outside of the pool boundary click the Start button or press the remote pushbutton Figure 3 8 Once the trial is started a red indicates where the software has determined the animal s location to be The Start Time field will be filled in with the current date and time and the trial will be highlighted in red If the Show Swim Path checkbox is filled a red line will trail the animal indicating its swim path The trial will automatically stop when either the animal remains on the platform for the user defined Platform Duration or when the user defined Max Swim Duration has been met Clicking the Stop button or pressing the remote pushbutton Figure 3 8 will also end the trial MED ASSOCIATES INC WATER MAZE VIDEO TRACKING The trial will be highlighted in yellow once it is complete and the next trial will automatically be highlighted in blue This allows the user to run every trial simply by pressing the remote pushbutton A completed trial will also have a Start Date amp Time and a Duration in the Main Screen s Trial List On the Editing Pr
8. 2 000 30 000 2 000 Yes 12 200 1 4 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 if 1 4 a 2 platfor On De 2 000 30 000 2 000 12 200 4 Modify Run Today 2 platfor On De 2 000 30 000 2 000 Yes 12 200 1 4 Modify Day 2 platfor On De 2 000 30 000 2 000 Yes 12 200 E 1 5 Modify Trial 3 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 1 5 Modify Comments 3 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 1 5 Modry Mex Saim Dra 3 platfor On De 2 000 30 000 2 000 Yes 12 200 Fo 1 5 bisten Aa lise based gt sistfor OnDe 2000 30 000 2 000 Yes 12 200 1 5 Start Section tfor On De 2 000 30 000 2 000 Yes 12 200 C 1 6 Platform Position b y2 tfor OnDe 2 000 30 000 2 000 Yes 12 200 Eo E pi 3 ee ee ae ee a a z r Or n DE es o 1 6 Poan el nee ere y tfor OnDe 2 000 30 000 2 000 Yes 12 200 O a 6 Modify Platform Dwell Time Random tfor OnDe 2 000 30 000 2 000 Yes 12 200 C 1 7 Modify Platform Dwell Continuous 4 OnDe 2 000 30 000 2 000 Yes 12 200 1 7 Modify Platform Include Radius 4 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 1 7 Copy Selected Trial 4 platfor OnDe 2 000 30 000 2 000 Yes 12 200 1 7 Delete Selected Trial 4 platfor OnDe 2 000 30 000 2 000 Yes 12 200 C 1 7 L 4 platfor On De 2 000 30 000 2 000 Yes 12 200 33 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Applying the Template to the Animals Select the
9. 2 Training 02 00 0 4 6 10 2008 14 29 02 00 0 01 41 2 2169 723 18 19 06 10 08 14_33_11 C5 3 wmv 4 1 05 3 Training 02 00 0 4 6 10 2008 14 33 00 28 5 00 26 5 513494 19 2 06 10 08 14 34 46 C5 7 4 wrny 4 1 Co 4 Training 02 00 0 4 6 10 2008 14 34 00 25 5 00 23 6 521 665 22 06 10 08 14_36_22 C57 5 wmv 4 1 C5 5 Training 02 00 0 4 6 10 2008 14 36 02 00 0 00 00 0 2243 136 16 6 46 Exporting the Swim Path The swim path can be exported in either Adobe Acrobat pdf or Microsoft Excel xls formats The swim path is translated into data points that are represented as polar coordinates that are named Radius and Angle The radius is the distance in centimeters from the center of the pool and the angle is in radians This is represented in the diagram in Figure 7 5 To export the swim path select Export Swim Path An Export Options screen similar to the one shown in Figure 7 2 will appear Select the Format and Destination as described in the Exporting Options section of this manual then click OK An example swim path report in PDF format is shown in Figure 7 6 Figure 7 5 Diagram of Radius and Angle Values lt Angle Radius Figure 7 6 Example Swim Path Report in PDF Format time ms radius cm angle 9 00 55 33 Zeal 67 53 50 2 49 jee Pee 33 3 02 2 49 200 00 51 508 2 47 266 67 51 49 eee Ja 33 51 10 2 46 400 00 50 5 25 EZ 466 67 50 01 2 39 5 50 43 2 3 500 00 51 20 2 36 f a3 iw 4 2 30 7 51 28 2
10. 30 000 2 000 Yes 12 200 1 3 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 Yes 12 200 Species E 1 3 14 0 Testing 120 3 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 1 8 14 0 Testing 120 3 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 2 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 2 3 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 Strain E 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 E 2 3 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 E 2 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 Birth Date 3 3 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 E 3 3 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 ae o f E 3 3 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 E 3 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 Weight E 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 E 4 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 4 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 9 8 14 0 Testing 120 4 plattor On De 2 000 30 000 2 000 Yes 12 200 E 9 3 14 0 Testing 120 4 platfor On De 2 000 30 000 2 000 Yes 12 200 L 3 14 0 Testing 120 4 platfor OnDe 2 000 30 000 2 000 Yes 12 200 E 8 14
11. 36 300 00 51 7 2 37 933452 53 0C 2 35 1 000 00 53 43 2 J Men na MED L LY NIE V V n V CIATES Exporting Video Exporting Swim Path To export the swim path of a trial to a video file select Export Video Swim Path The screen shown in Figure 7 7 will appear Select the desired video file and click Open Figure 7 7 Open Video File My Recent Documents My Computer My Network HE 06 10 06 14 22 32 C57 06 10 08 14_24_ 03 C57 06 10 08 14_27_33 C57 06 10 08 14_209_22 C57 HE 06 10 08 14233_11 C57 06 10 08 14_34_46 C57 06 10 08 14_36_22 C57 06 10 08 14_39_27 C57 06 10 08 14 41_03 C5 HE 06 10 08 14 42_26 C57 06 10 05 14_43_51 C57 06 10 06 14_45_31 C57 06 10 08 14_47_57 C57 E 06 10 08 14_50_23 C57 06 10 08 14_51_51 C57 ki File name Files of type 06 10 08 14_53_31 C57 E 06 10 06 14_54_46 C57 E 06 10 06 14_56_55 C57 06 10 08 14_57_59 C57 E 06 10 08 14_59_12 C57 8 06 10 08 15_00_09 C57 8 06 10 06 15_01_15 c57 06 10 08 15_03_18 C57 E 06 10 08 15_04_04 C57 06 10 08 15_06_17 C57 E 06 10 08 15_07_26 C57 E 06 10 06 15_08_33 C57 E 06 10 08 15_11_53 C57 06 10 08 15_13_24 C57 8 06 10 06 15_14_31 C57 Video Files rn w E 06 10 E 06 10 E 06 10 E 06 10 E 06 11 E 06 11 E 06 11 E 06 11 E 06 11 E 06 11 E 06 11
12. Ce The screen shown in Figure 7 8 will appear Select the destination folder enter a file name and click Save When the video file is opened and played it will appear as shown in the Microsoft Windows Media Player example in Figure 7 9 Figure 7 8 Enter a Video File Name Save in 4 My Recent Documents My Network i ap test E 06 10 08 14 22 52 Co E 06 10 08 14 24 03 Co E 06 10 08 14 27_ 33 C57 HE 06 10 08 14 29 22 C57 E 06 10 08 14 33 11 Co 06 10 08 14 34 46 C57 AB 06 10 08 14_36_22 C57 06 10 08 14_39_27 C57 06 10 08 14_41_03 C57 06 10 08 1442 26 C57 06 10 08 14_43_51 C57 06 10 08 14_45_31 C57 06 10 08 14_47_57 C57 06 10 08 14_50_23 C57 06 10 08 14_51_51 C57 x File name Save as type E 06 10 08 14 53 51 c57 E 06 10 08 14 54 46 C57 06 10 06 14_56_55 C57 HB 06 10 08 14 57_59 C57 E 06 10 08 14 59 12 CoP 06 10 08 15_00_09 C57 06 10 08 15 01_15 C57 06 10 08 15_03_18 C57 06 10 08 15_04 04 C57 06 10 08 15_06_17 C57 06 10 08 15_07_ 28 C57 06 10 08 15_08_33 C57 06 10 08 15_11_53 C57 06 10 08 15_13_24 C57 06 10 08 15_14_31 C57 Video Files wen bl Sawe Cancel MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Figure 7 9 Example Exported Swim Path Video Exporting Video with Swim Path To export the video with swim path of
13. File Edit View Export Trial EQS Video Help Load Settings wv Show Swim Path Thigmotaxis Moser s Zone Wishaw s Corridor Sections Menu Item Description For More Info Reanalyze Reanalyze the trial data ed Ea d Settings Chapter 6 Loading Analysis Load Settings Load the analysis settings f me ya Ing Settings Save Settings Save the analysis settings Chapter 6 Saving Analysis Settings Show Swim Se as Path Show the animal s swim path during the trial Chapter 5 Starting a Trial Thigmotaxis View or change the default Thigmotaxis setting Chapter 5 Thigmotaxis Moser s Zone View or change the default Moser s Zone setting Chapter 5 Moser s Zone Wishaw s g Carne View or change the default Wishaw s Corridor setting Chapter 5 Wishaw s Corridor Sections View or change the Sections settings Chapter 5 Modify Pool Settings Video Menu Figure 4 7 Video Menu File Edit View Export Trial Analysis Configure Camera w MED Associates 4602F BL Menu Item Description For More Info Opens the Calibrate Camera screen Configure Camera Appendix A il r Figure A 2 PP Camera Name Selects the default camera N A Help Menu Figure 4 8 Help Menu File Edit Export Trial Analysis video view About Water Maze Menu Item Description For More Info Displ h f isi About Water Maz isplays the software revision and N A copyright information Figure 4 9 Figure 4 9 About Screen About Water Maze Wate
14. Ma MENU E E E A R 16 PUAN SIS CaO ce ences ase O E E E O E E A OT 16 Vdeo MENU sra E E OE E E A ATES 17 PVCU MIST rs tiara risa 17 EdICPro ect Menu Da asin orar 18 Pile M DU serrat anotaciones cria cases 18 Tha Men Mari iia 18 Edit Templates Menu Baf nia ca 19 Template Me Utuado sonas 19 Chapter D nn 20 SP 20 Naming or Selecting ProjeCt ococccccccccccccccccnnccnncnnn cnn nn cnn 20 Editing POOL Parameters moises piro eb ooo 21 Modify POOL SOLOING inercia iio 21 Modify PlatforiMsS serrano redondo ii dandoni acatar caoacds dades 23 TOIIOMOL IS desicion havssen reactor osas ANT 25 MOSES LONC asa a a acacia 25 Wishaw s Corridor starter lar datada di diia dar 26 Save POD ParameterS serrat rre erica tarjeta 26 Setting Pre TerencOS sra 27 Adding Animals to Project ccococcccococnnnccconacnoran nora rnanar nora nro narran rro rar nora rnanannnnas 28 Creating a Trial Template saciar ene 30 Template VV Gree sacs sce cress eus neina iaa e AAA 30 Applying the Template to the Animals ccccccccccee ee esses seen eee eeaeeee eee esaeeeeeenees 34 Stab eel ai Trial ita 35 Chapter iia 37 Opening an EXISTINO PO O Olsosaasnsiaccnn csi 37 Video Playback CONTO sciences 39 Changing Analysis Settings ore rasca 39 Saving ANalySiS SOLOS mirrsrrsiiiiikr inina 39 Loading Analysis Settings lt sci iditsdcnsovcaasidkctsswasasncnesidetedwnneycaneadsatiamasavesataaweaavas 40 Reana a Farr nE EEE EE EEEO 40 RENNA a Talaia iia 40 Editing
15. Parameters C Thigmotaxis Distance From pool wall 5 0 Jon Moser s Zone Diameter cm 3 Lock Settings C Wishaw s Corridor Modify Pool Settings Lean i with Lo Jen O Modify Platforms Use Platform Width Add Platform Delete Platform Number of Start Sections 4 Rotate Start Sections Use Start Section with Random Sections 25 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Wishaw s Corridor To view or change the default Wishaw s Corridor setting check the Wishaw s Corridor checkbox The default setting will be indicated on the screen by a white rectangle as shown in Figure 5 12 Enter the corridor width in cm in the Width field or select the Use platform width checkbox Figure 5 12 Wishaw s Corridor Default Setting Edit Pool Parameters C Moser s Zone Diameter cm wishaw s Corridor Width 12 2 cm Use Platform Width Lock Settings O Modify Pool Settings O Modify Platforms Add Platform Delete Platform Number of Start Sections 4 Rotate Start Sections Use Start Section with Random Sections Save Pool Parameters To save the pool parameters and continue setting up the experiment select Lock Settings and click Exit as shown in Figure 5 13 The screen Editing Project screen shown in Figure 5 15 will appear Figure 5 13 Lock Pool Settings and Exit Edit Pool Parameters Pool Diameter cm C Moser s Zone Diameter
16. Shutter settings will depend on whether the software will be tracking a light animal on a dark background or a dark animal on a light background In both cases it is desirable to have maximum contrast between the animal and the pool The grayscale histogram on the Calibrate Camera screen contains video data from inside the pool boundary only and does not include the video inside the platform boundary The camera calibration options are as follows e Brightness Adjusts the black level of the camera e Gain A multiplier for the camera sensor signal output e Shutter Determines how long the camera sensor is exposed during each frame capture Figure A 2 Calibrating the Camera Calibrate Camera A mE Standard Deviation BME Brightness Gain Shutter 1000 000 3000 NOTE It is important to note that these steps are MEANT TO BE USED AS A GUIDELINE and that fine tuning may still be necessary The Brightness Gain and Shutter may need adjusting based on the color of the animal Tracking a Light Animal on a Dark Background When tracking a light animal on a dark background the image should be slightly underexposed as shown in Figure A 3 This means that the grayscale histogram on the Calibrate Camera screen should be shifted to the left The example below illustrates settings that may be desirable Figure A 3 Example Settings for Tracking a Light Animal on a Dark Background Calibrate Camera Average Intensity
17. Standard Deviation Brightness U I U ben 600 800 1000 Gain er 150 200 250 Shutter 2000 3000 Cancel MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Tracking a Dark Animal on a Light Background When tracking a dark animal on a light background the image should be slightly overexposed as shown in Figure A 4 This means that the grayscale histogram on the Calibrate Camera screen should be shifted to the right The example below illustrates settings that may be desirable Figure A 4 Example Settings for Tracking a Dark Animal on a Light Background A 4 Example Settings for Tracking a Dark Animal on a Light Background Calibrate Camera r mE Standard Deviation Brightness Sareea eal 200 600 300 1000 Gain a ee n ll ee et a ME 0 50 100 150 200 ai Shutter Al 1 1 I 1 E E 1000 2000 3000 52 APPENDIX B Driver and Software Installation NOTE Software installation requires a password that is issued during software registration If the computer being used was purchased as part of the Water Maze Video Tracking system from MED Associates the driver and software installation was completed at the factory If the computer was not purchased from MED Associates follow the instructions to install the hardware drivers and software programs Before beginning the installation phone fax or e mail Med Associates with the registration information in order to receive the software installation password
18. animals from the list to apply the template to To select all the animals on the list click on the animal at the top of the list then hold down the shift key and click the animal at the bottom of the list or click any animal in the list and press CtrI A Right click on the highlighted animal s or click the Animal menu and the menu items shown in Figure 5 23 will appear Figure 5 23 Apply Template to Selected Animals E Editing Project text File Edit wiew Animal Trial Help Project Name Edit Pool Platforms Add Animal Edit Animal Add Animal RunT D Trial Animal Comm Platfor Platfor Durati Raise Dwell Dwell Indud amp Gender ALAS Add Animal manual template Edk Selected Animals i o 7 ardis Delete Selected Animals Strain Birth Date weight v Select Apply Template and then select the template to apply to the animals The Trial List will then be populated with the trial information as shown in Figure 5 24 Figure 5 24 Trial List Populated E Editing Project text File Edit View Animal Trial Help Project Name Edit Pool Platforms Animal List Trial List Run T Trial Animal Comm MaxS StartS Platfor Platfor Durati Raise Dwell Dwell Includ Gender E 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 Yes 12 200 E 1 8 14 0 Testing 120 3 platfor On De 2 000
19. click the Load Settings button and the Select Analysis screen shown in Figure 6 7 will appear Select the analysis settings to apply to the project from the pulldown menu and click OK The settings entered in the Edit Pool Parameters screen see the Editing Pool Parameters section of this manual are named Pool Settings Figure 6 7 Select Analysis Screen Select Analysis Reanalyzing a Trial To apply the new analysis settings to a completed trial highlight the desired trial and click the Reanalyze button The data will be reanalyzed and displayed using the new analysis settings Rerunning a Trial If for any reason the data recorded during a trial is incorrect for example if the trial was started prematurely the trial can be rerun The data recorded will be deleted and replaced with new data To rerun a trial highlight the desired trial and select Trial Rerun The prompt shown in Figure 6 8 will appear Click Yes to proceed Figure 6 8 Rerunning Trial Prompt MM Rerun Trial A Warning Rerunning a trial will erase the existing trial s data Are you sure you want to rerun the trial Yes MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Editing the Animal s Position If the animal was not properly tracked during a trial its position can be edited Highlight the completed trial to be edited and select Trial Edit Animal s Position The confirmation prompt shown in Figure 6 9 will appear Click Yes to proceed and the
20. cm Lock Settings C Wishaw s Corridor Modify Pool Settings Sa i with 12 2 Jem O Modify Platforms Use Platform Width Add Platform Delete Platform Number of Start Sections 4 Rotate Start Sections Use Start Section with Random Sections 26 Setting Preferences Prior to starting a trial set the tracking preferences and video file storage location by selecting Edit Preferences The screen shown in Figure 5 14 will appear Select the color and species of animal that will be run in order to optimize the tracking algorithm It is recommended that the Either option be selected only if both dark and light colored animals will be used in the same project After verifying the Maximum Swim Duration and the Data Directory click OK to save and close If at any time during the project the color or species of animal changes it is recommend that the Preferences settings be changed accordingly Figure 5 14 Preferences Screen Preferences Tracking Mouse Default Maximum Swim Duration Seconds Light Rat O Either Default Data Directory Ci Documents and Settings Drew My Documents MED Associates Water Maze MED ASSOCIATES INC Adding Animals to Project WATER MAZE VIDEO TRACKING To begin adding animals click the Add Animal button on the Editing Project screen This will open the Add Animal screen shown in Figure 5 16 Figure 5 15 Editing Project Screen Editing Project test 4
21. main screen will appear as shown in Figure 6 10 Figure 6 9 Editing Animal s Position Prompt MM Edit Animals Position A Warning Editing an animal s position will permanently change the data Are you sure you wank to edit the trial The selected trial will be highlighted in red There are two editing modes If the Recalculate Animal s Position checkbox is checked the software will automatically relocate the animal If it is unchecked the user may set the animal s location for each frame Figure 6 10 Edit Animals Position pa a Stalbans TempShar Start Water Maze 06100 Water Maze 061008 C57 Testing x Start Time 13 34 01 06 13 08 Duration Latency to platform Distance Traveled 0 000 lem Average Velocity oo00 ems Gallagher s Proximity AAA Show Swim Path Thigmotaxis Distance from pool wall 10 0 Jem Moser s Zone Diameter 20 0 cm Wishaw s Corridor o Jia 11 0 Jem Use Wid Platform ith Sections Time in Start Section 4 0 0 Number of Sections Rotation 180 Use Start Locations Reanalyze Save Settings Load Settings Settings I Recalculate Animals Position Video PIES E 44 44 DD DD 00 00 000 00 33 400 x1 00 D isptesti06 13 08 13_34_01 C57 4 wmw Show All Trials Select Trials Animal Comments Start Section Platform Position Start Date amp Time Duration Training Section 2 Atla
22. the Animal s Position ccccceeceee ee eeee eee eseeeeeeeaeeeeeesaeeeeesaaeeteeganeeeegaanes 41 Automatically Recalculating the Animal s POSition cccccceseeeeeeeeeeeseeeeeeeeesaaas 42 Manually Recalculating the Animal s Position coccccccccccconcnncncncnnnnnanrnnnnnn nano 42 Chapter 7 43 EXPONO Dala ciaventeceseteccutavendceceusedeceesesctemeeceteetecus erence reeds AENA RSA 43 Generating RGD OMS iasicionssssanio ici 43 4010 glo O oo AMP a 44 Example Report in PDF FOrimat cccscccccesscceresseceetesaceerenseseetagasserengenertagasneres 45 Example Report in CSV TTX Format cccecesscceetescceerenseeeetesaceerengetentagaeeeres 46 Exporting the Swim Path sissscccasssasceeeeansdedeserenssnnweedserasaatssatennesoaeageunieamneaseesaenns 47 EXDORNO VIGO misas bes 48 EXPONO SWIN PA cori adan ias 48 Exporting Video With Swim Path cccccceeececceee eee esses eee eee nr rn 49 Append Aa 50 Configuring the Camera esse 50 Adjusting Camera Zoom FOCUS and Aperture cccceeceeeseeeeeeeeeeseeeeeeeeeesguenteenees 50 Calibrating the Camera ccicisiciivessenshoinsensensvaadenspentteiussaacnneteaeseasstaedbeerseonisenseeds 50 Tracking a Light Animal on a Dark Background ooccccccccccccccccnnnnnccncnnnnnnnnnnnnanns 51 Tracking a Dark Animal on a Light Background ccccceceeeeeeeeeeeeeeeeeeeesaneeeeaas 52 APDO Dor 53 Driver and Software Installation cccccccccecccacccecccucececccecceeuceeuutsuusnun
23. 0 0 1949387 16 2 5 06 10 08 13_52 06 C57 4 wmv 1 1 C57 4 Training 02 00 0 4 6 10 2008 13 52 01 09 0 01 07 1 1361 747 20 2 6 06 10 08 13_54_48 C57 5 wmv 1 1C57 5 Training 02 00 0 4 6 10 2008 13 54 00 59 6 00 57 7 1104 256 18 06 10 08 13_57_51 CS7 1 wmv 2 1 C57 1 Training 02 00 0 4 6 10 2008 13 57 00 22 5 00 20 5 562 567 27 2 6 06 10 08 13_59_11 C57 2 wmv 2 1 C57 2 Training 02 00 0 4 6 10 2008 13 59 02 00 0 00 00 0 2613 046 21 7 9 06 10 08 14_02_29 C57 3 wmv 2 1 05 7 3 Training 02 00 0 4 6 10 2008 14 02 01 55 7 01 07 7 2118 725 18 10 06 10 08 14_07_ 08 CS7 4 wmv 2 1 C5 7 4 Training 02 00 0 4 6 10 2008 14 07 02 00 0 00 00 0 2203 744 19 0 11 06 10 08 14_10_52 C57 5 wmv 2 1105 7 5 Training 02 00 0 4 6 10 2008 14 10 00 35 8 00 10 6 687 698 20 2 12 06 10 08 14_13_15 C57 1 wmv 3 1 Co 1 Training 02 00 0 4 6 10 2008 14 13 00 06 3 00 04 4 130 927 26 6 13 06 10 08 1414 33 C57 2 wmy 3 1 C57 2 Training 02 00 0 4 6110 2008 14 14 02 00 0 00 17 7 2467 5644 20 6 14 06 10 08 14_18_21 C5 3 wmv 3 1 05 3 Training 02 00 0 4 6 10 2008 14 16 02 00 0 00 00 0 1898 4 15 15 06 10 08 14_22_32 CS7 4 wmv 3 1 C57 4 Training 02 00 0 4 6 10 2008 14 22 00 30 8 00 26 9 655 263 22 6 16 06 10 08 14 24 03 C57 S wmy 3 1 057 5 Training 02 00 0 4 6 10 2008 14 24 01 16 4 01 14 5 1536 674 20 5 17 06 10 08 14 27_33 CS7 1 wmv 4 1 C5 1 Training 02 00 0 4 6 10 2008 14 27 00 34 6 00 27 3 065 014 26 2 18 06 10 08 1429 22 CS7 2 wmv 4 1 C5 7
24. ED ASSOCIATES INC WATER MAZE VIDEO TRACKING Thigmotaxis To view or change the default Thigmotaxis setting check the Thigmotaxis checkbox The default setting will be indicated on the screen by an orange circle as shown in Figure 5 10 This value can be changed by entering a value in cm in the Distance from pool wall field or by placing the cursor over the perimeter of the orange circle until it changes to a then clicking and dragging until the circle is the desired distance from the pool wall Figure 5 10 Default Thigmotaxis Setting Edit Pool Parameters Pool Diameter cm 120 650 r Distance from pool wall 5 0 em C Moser s Zone ss Diameter cm Lock Settings C Wishaw s Corridor Modify Pool Settings Kad y wah Lo Jem O Modify Platforms Use Platform Width Add Platform Delete Platform Number of Start Sections 4 Rotate Start Sections Use Start Section with Random Sections Moser s Zone To view or change the default Moser s Zone setting check the Moser s Zone checkbox The default setting will be indicated on the screen by an orange circle as shown in Figure 5 11 This value can be changed by entering a diameter in cm in the Diameter field or by placing the cursor over the perimeter of the orange circle until it changes to a 4 then clicking and dragging until the circle is the desired diameter Figure 5 11 Default Moser s Zone Setting Edit Pool
25. File Edit View Animal Trial Help Project Name Animal List Gender Species Strain Birth Date Weight Figure 5 16 Add Animal Add Animal Mame Animal Gender Male e Species wt Strain wt Birth Date e Edit Pool Platforms Day Trial An Co The following information may be entered in the Add Animal screen The only required field is number of animals Add Animals Enter the number of animals to add Note that if more than one animal is added at once they will all have the same gender species strain birth date and weight NOTE This is the only required field on the screen all other fields are optional 28 Animal Name Prefix Suffix Gender Species Strain Birth Date Weight Enter the desired animal name This will appear on the Animal List on the Edit Project screen If more than one animal is being added the animals will be named using the Prefix then the number then the Suffix If only one animal is being added the animal will be named using Name only To produce the animal names shown in Figure 5 17 the date was entered in the Prefix field and the letter A was entered in the suffix field Enter the desired suffix This field is only enabled if more than one animal is being added Select male or female Select mouse or rat Enter the animal strain Enter the animal birth date Enter the animal s weight Once all of the necessary
26. This password will be necessary during the installation process Insert the Water Maze Video Tracking CD into the CD ROM drive The screen shown in Figure B 1 will appear Click Install Water Maze and the screen shown in Figure B 2 will appear Figure B 1 Water Maze Video Tracking CD Main Screen a Water Maze E Me 1 ASSOCIATES ING Welcome to Water Maze Install Water Maze Extras Browse the CD E wit MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Begin installing the drivers and software by clicking Install Complete the steps to install the drivers and software entering the desired User Name and Company as well as the password when prompted Successful installation of each item will be indicated by a green check mark and a red X will indicate an unsuccessful installation Once the drivers and software have successfully been installed the screen shown in Figure B 3 will appear Figure B 2 Driver and Software Installation Checklist Water Maze Install Wizard med ASSOCIATES ING Welcome to the install wizard for Water Maze The wizard will install Water Maze on your computer To continue click Install DIG 703 USB Driver Fire Wire Camera Driver Med Test Water Maze instrumentation and software for research Cancel Driver and software installation has been successfully completed Click Finish to close this screen Figure B 3 Driver and Software Installat
27. WATER MAZE VIDEO TRACKING SYSTEM USER S MANUAL SOF 845 User s Manual DOC 214 Rev 1 1 Copyright 2009 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC WATER MAZE VIDEO TRACKING TABLE OF CONTENTS Chapter Locarno 1 giga A o Maviaakeanaaacas 1 EURO e E A O 1 Aa Em oo E 2 CID CSE nr cl 3 Water Maze System HardWarF8 occcccccccccccccnnnnnnnnnnnnn nr nn rn nr 3 General Computer ENvirOnMent ccccceecenssccceeeennssccceeeeennscceeeretennnseeenentannaegs 3 Aardware GUIGE asar via 3 Standard Mahal Cris ica dt dies ciedad 3 opona PIAL CVO E rostro 4 Cilla DUSK Sid odie EO EEEE EEEO 7 Cam A e A EU 7 Installing the Camera MOUN ccccscccccesccceresseceetanaceeeressereetasaeseresgesertagastetssaanes 7 SNEECE ROCK COMING siria 7 Drop Ceiling INStrUuCctiOnS ccccecenssccceeeeenssceceeeeeennseceseeeennsaceeeeetnnsecssenennngags 8 Leveling the Optional Pool Support sccccnicesiacavesecisastvoiexaasvas wesdentaneseeitaidacerenedaaneen 9 ASsemMmDING HardWare mesias ic 10 Wiring the SY SECM cancnuneassnanaedus errat ihavgeccanewaadiiasactiaes sede teiesonesauatcontenaneeastanenss 11 FURO TING PPP tnewveweisaaes niaunteeseenieuutmeucecuieencnervaueneen 13 Chapter dnd 14 WSOP IMEN aC siii ida eii 14 Malin Screen Menu Bal astra 14 Pe Mentor ec caiaas 14 EOR MENU rra aa 14 VIEW Men lso tr 15 EXDORE MONU eeaeee tania latas E r E a cines 15
28. a trial to a video file select Export Video Video with Swim Path The screen shown in Figure 7 7 will appear Select the desired video file and click Open Then the screen shown in Figure 7 8 will appear Enter a file name and click Save When the video file is opened and played it will appear as shown in the Microsoft Windows Media Player example in Figure 7 9 Figure 7 10 Example Export Video with Swim Path Video Now Playing exported video with swim path 49 NE ACCHRTIATOCC NIF AATED MATE VTINCNH DAFvVUTNRIiF VI FD ASSOCIA E S N WA E R MAZE VI YE I RAC K N LD IJUN 1 11 LL LINN VVM TITLA PIAL V LOU L OJ I INFANIAL I LJ J APPENDIX A Configuring the Camera Adjusting Camera Zoom Focus and Aperture Open the Water Maze Video Tracking software application and click View Main Screen To adjust the camera focus or zoom loosen the thumbscrew on the ring and make the necessary adjustment Once proper image clarity is achieved tighten the thumbscrew on the ring It is recommended that the lens aperture in the maximal open position Figure A 1 Focus and Aperture Rings on Video Lens Calibrating the Camera Open the Water Maze Video Tracking software application Click View Main Screen then click Video Configure Camera and the screen shown in Figure A 2 will appear With the main screen in the background the camera image will be visible The Brightness Gain and
29. anually Recalculating the Animal s Position To manually recalculate the animal s position uncheck the Recalculate Animals Position checkbox Use the slider bar to locate the frame in the video to retrack the animal and click on the animal This will replace the animal s location data for this frame only To retrack the animal in the next frame click on the slider bar and use the left and right arrow keys on the computer keyboard to advance the video one frame at a time Click on the animal in each frame where the animal s location data should be replaced When done click the Stop trial button The animal s position should now be properly tracked 4 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING CHAPTER 7 Exporting Data Generating Reports To generate reports select Export Generate Reports The Select Trials for Reporting screen shown in Figure 7 1 will appear Note that only completed trials will appear on this list Select the Report Options at the bottom of the screen The information included in the report depends on the report options enabled If Display Time in Seconds is unchecked the time will be displayed in MM SS format and if it is checked all times will be in seconds e g 2 minutes 11 seconds will appear as 131 sec Highlight the desired trials use Ctrl A to select all and then select the Analysis Settings to apply to the exported data Then click Generate Report The Export screen shown in Figure 7 2 will app
30. ar are the same as those on the Main Screen the only exceptions are the Template and Trial menus The Template Menus is shown below and the Trial menu is explained above in the Edit Project Menu Bar section Template Menu Figure 4 12 Template Menu File Edit view Trial Help Menu Item Wizard New Copy Rename Delete Import Wizard Mer Copy Rename Delete Import Description Open the Template Wizard Figure 5 19 Add a new template to the template list on the Trial Templates screen Figure 5 21 Copy the highlighted trial template Rename the highlighted trial template Delete the highlighted trial template Import a trial template from another project For More Info Chapter 5 Creating a Trial Template Can also be accessed by right clicking a trial template Can also be accessed by right clicking a trial template Can also be accessed by right clicking a trial template Can also be accessed by right clicking a trial template MED ASSOCIATES INC WATER MAZE VIDEO TRACKING CHAPTER 5 Starting a New Project Naming or Selecting Project Begin a new project by double clicking on the Water Maze Tracking Software shortcut on the desktop The Select Project Wizard shown below will appear Click on New Project and the screen shown in Figure 5 2 will appear Figure 5 1 Select Project Wizard Select Project Wizard ES Mew Project Load Project Open this wizard automaticall
31. cm Platform Diameter 6 0 15 2 cm Features Textured surface facilitates animal climbing onto platform DODO JOOOOOOOUOOOOOOUOOUOO0O Ojal OOO ian O00 se OO F OO Oo TENE OOOOUOOOOO00 OOO OOO ojal OOO OOO al OO O OOOODOOOOOUOURO0000 OODOOOOOUOOOU0O At r al E E aja OO JOODDOORO00000000000080 DOOOOOOOOOOOODOOOO00 El q A a E o oo oo a O o g z m o o z a Oo MED ASSOCIATES INC WATER MAZE VIDEO TRACKING ENV 595M Mouse On Demand Platform Figure 2 9 ENV 595 On Demand Platform Dimensions Platform Top Base 8 0 W x 8 0 D 20 3 x 20 3 cm p o Height dt Raised 14 75 H 37 5 cm Lowered 8 75 H 22 2 cm Platform Diameter 4 0 10 2 cm Features Textured surface facilitates animal climbing onto platform ENV 595R Rat On Demand Platform Pro Dimensions Base 8 0 W x 8 0 D 20 3 x 20 3 cm Height Raised 14 75 H 37 5 cm Lowered 8 75 H 22 2 cm Platform Diameter 6 0
32. e Start Sections can be rotated by entering a value in degrees in the Rotate Start Sections field Next select which Start Sections to include in the selection of Random Sections Generally every section but the one containing the platform should be selected 22 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Modify Platforms Click Modify Platforms to enable the platform settings options The screen will appear as shown in Figure 5 6 Click Add Platform and the Add Platform Name dialog shown in Figure 5 7 will appear NOTE At least one platform must be added in order for data analysis to function properly Figure 5 6 Modify Platform Settings Edit Pool Parameters C Moser s Zone Diameter 20 0 O Lock Settings C Wishaw s Corridor O Modify Pool Settings Width 1 0 cm Modify Platforms Use Platform Width Add Platform Delete Platform Number of Start Sections 4 Rotate Start Sections gt Use Start Section Enter the desired platform name and click OK The screen will appear as shown in Figure 5 8 Figure 5 7 Add Platform Name Add Platform Name Enter Platform Name D Cee 23 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING The green circle that appears in the center of the screen represents the platform boundary Place the cursor inside the circle until it changes to a 4 then click and drag the green circle is directly over the platform location as shown in F
33. ear Figure 7 1 Select Trials for Reporting Screen Select Trials for Reporting Animal Comments Start Sec Platform Start Dat Duration Section2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Section 2 06 10 08 Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training Training M ee pe 0 0 0 0 rm om 24 Y MN Oh ON ARIS A O SA SO SN Ap HDHD HD HD HD HD HD SL A ee O O O OS omnia a l Qu 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 4 Report Options Platforms Thigmotaxis Wishaw s Corridor Display time in seconds Sections Moser s Zone Gallagher s Proximity Analysis Settings 98 12 08 09_09_26 v Generate Report
34. eeanennnenannnags 53 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING CHAPTER 1 Introduction System Overview The MED Associates Inc Water Maze Video Tracking System uses a video camera to capture video of an animal swimming in the water maze The Water Maze Video Tracking software uses this video to track the animal s swim path and analyze swim patterns The video is stored on the computer The Water Maze Video Tracking software features an intuitive database that allows full control and management of protocols and study design The MED Associates Inc Water Maze Video Tracking Software tracks the animal s location in the water maze The software can be programmed to raise the On Demand Atlantis Platform when the animal is within the Include Radius for a user defined duration This is illustrated in Figure 1 1 Figure 1 1 Raising the On Demand Platform Using the Water Maze Video Tracking Software Include Radius Platform Raised Platform I I Lowered Animal remains inside the Include Radius for the specified time Animal swims over platform location Platform Lowered Key Terms e Dwell Time e Include Radius e Moser s Zone e Platform Crossing e Probe Trial e Start Sections e Thigmotaxis e Training Trial e Wishaw s Corridor e Gallagher s Proximity The amount of time that an animal must remain in
35. eed can be adjusted using the Increase Decrease Playback Speed keys indicated below Figure 6 5 Video Playback Controls Labeled STOP VIDEO amp DECREASE ADVANCE RETURN TO PLAYBACK TO END BEGINNING SPEED OF VIDEO PLAYBACK SPEED a i i j y Recalculate Animals Position b E ldd 44 b gt PP 00 03 200 00 18 333 x1 00 Disp test 06 15 08 11_49_46 C57 4m PLAY RETURN TO INCREASE CURRENT LOCATION VIDEO FILE NAME PAUSE BEGINNING PLAYBACK IN VIDEO TOTAL VIDEO OF VIDEO SPEED DURATION OF VIDEO Changing Analysis Settings The Show Swim Path Thigmotaxis Moser s Zone Wishaw s Corridor and Sections settings used to analyze the data of a completed project can be changed and the data can be reanalyzed using the new settings To alter an analysis parameter e g the Moser s Zone diameter fill the check box next to the parameter in the Main Screen s Analysis window This will enable the parameter s edit box for adjustment After changing an analysis setting press the Reanalyze button to recalculate the percentage or counts Saving Analysis Settings To save new analysis settings click the Save Settings button and the Save Analysis screen shown in Figure 6 6 will appear Enter the desired Analysis Name and click OK These settings will be applied to the entire project Figure 6 6 Save Analysis Save Analysis Enter Analysis Name Loading Analysis Settings To load previously saved analysis settings
36. emand 2 000 30 000 2 000 Yes 12 200 4 3 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 4 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 5 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 6 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 7 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 8 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 9 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 10 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 1 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 2 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 3 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 4 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 5 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 6 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 7 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 8 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 9 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 5 10 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 32 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Figure 5 22 Modify Trial Settings Editing Project text File Edit View Animal Trial Help Project Name text Ed
37. eport Also Display Time in Seconds was enabled so the Trial Duration is displayed in seconds rather than HH MM SS format Figure 7 3 Example PDF Report tk Day 1 Trial 1 Animal C57 1 di Filename Trial Day Animal Comments Maximum Swim Duration Start 5 ion Dwell Continuous Standard Thigmotaxis Distance cm Gallagher s Proximity MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Example Report in CSV TTX Format Reports generated using CSV or TTX format can be viewed in Microsoft Excel and will appear as shown in Figure 7 4 Again the information included in the report depends on the report options enabled on the Select Trials for Reporting screen Figure 7 1 jT Figure 7 4 Example Report in CSV Format File Edit Yiew Insert Format Tools Data Window Help lx Dee 6AY BOS n o BE fw BF MB 100 gt anal W BLU EF32 2H 8 8 HE 8 O A Al z Trial Filename A B C D E F G H J K Cr 1 Trial Filename Trial Number Trial Start Day Animal s Name Trial Comments Maximum Swim Duration Start Section Trial Start Date Duration Latency to Platform Distance crn Average 2 06 10 08 13_33_09 C57 1 wmv 1 1 C57 1 Training 02 00 0 4 6 10 2008 13 33 02 00 0 00 00 0 2211 359 18 2 3 06 10 08 13_38_25 C57 2 wmv 1 11C57 2 Training 02 00 0 4 6 10 2008 13 38 02 00 0 00 00 0 2265 845 16 6 4 06 10 08 13_47_23 CS7 3 wmv 1 1 C57 3 Training 02 00 0 4 6 10 2008 13 47 02 00 0 00 0
38. er x 6 50 H 168 0 x 16 5 cm ENV 594M Mouse Pool Dimensions 48 0 Diameter x 24 0 H 122 0 x 61 0 cm Capacity Designed to hold up to 150 gallons of water ENV 594R Rat Pool Dimensions 72 0 Diameter x 25 0 H 183 0 x 63 5 cm Capacity Designed to hold up to 350 gallons of water ENV 593M Mouse Pool Support Dimensions 48 0 W x 16 0 20 0 H x 48 0 D 122 0 x 40 6 50 8 x 122 0 cm Weight 70 pounds Capacity Designed to hold up to 200 gallons of water ENV 593R Rat Pool Support Dimensions 70 0 W x 17 0 21 0 H x 70 0 D 177 8 x 43 2 53 3 x 177 8 cm Weight 120 pounds Capacity Designed to hold up to 600 gallons of water MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Figure 2 7 ENV 597 Heater ENV 597 Heater Voltage 120 V Power 1500 W Features Low Liquid Shut Off Ability to control temperature in C or F Temperature Range 30 to 220 F 35 to 105 C ENV 596M Mouse Fixed Platform Dimensions Platform Top Base 7 0 W x 7 0 D 17 8 x 17 8 cm OOOO Height H g Figure 2 8 ENV 596 Fixed Platform T Raised 15 0 H 38 1 cm Lowered 8 5 H 21 6 cm Platform Diameter 4 0 10 2 cm Features Textured surface facilitates animal climbing onto platform ENV 596R Rat Fixed Platform Dimensions Base 7 0 W x 7 0 D 17 8 x 17 8 cm Height Raised 15 0 H 38 1 cm Lowered 8 5 H 21 6
39. ges to a 4 then click and drag the circle to the desired location or using the lt gt 2 v buttons in the screen s upper left corner Figure 5 4 Modify Pool Settings Edit Pool Parameters Pool Diameter cm 1 lO Moser s Zone diadh Diameter cm ose Cl wishaw s Corridor modify Pool Settings Width 1 0 Jem O Modity Platforms Use Platform Width Add Platform Delete Platform HANDLE Number of Start Sections 4 Rotate Start Sections Use Start Section with Random Sections 21 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Once the pool boundary is in the desired location click the Measure Pool button and the Measure Pool dialog shown in Figure 5 5 will appear Measure the width of the pool at the water level in the location indicated by the orange line on the screen then enter this measurement and click OK Figure 5 5 Measure Pool Edit Pool Parameters C Thigmotaxis 120 650 Distance from pool wall 17 0 Jem gt C Moser s Zone A Diameter cm Lock Settings C wishaw s Corridor Modify Pool Settings Width 1 0 cm O Modify Platforms ro Use Platform Width Pool Diameter cm Ly Add Platform Delete Platform Measure Pool Number of Start Sections Rotate Start Sections Centimeters Use Start Section Enter the number of Start Sections see the Key Terms section of this manual The Start Sections are indicated in purple Th
40. igure 5 9 Figure 5 8 Move Platform Boundary Location Edit Pool Parameters Pool Diameter cm Diameter 20 0 O Lock Settings C Wishaw s Corridor Width 1 0 cm Use Platform Width O Modify Pool Settings Modify Platforms Add Platform Delete Platform On Demand Platform PLATFORM NAME AND SIZE Number of Start Sections 4 Rotate Start Sections U Use Start Section with Random Sections The size of the green platform boundary circle can be adjusted by placing the cursor over the perimeter of the circle until it changes to a then clicking and dragging until the green platform boundary circle is the desired size The name and diameter of the platform will be displayed on the left side of the screen The size of the platform is calculated using the pool diameter value entered by the user If the pool diameter was entered incorrectly this will affect the platform diameter calculation Multiple platforms may be added at this time Figure 5 9 Platform Boundary in Correct Location Edit Pool Parameters Distance from pool wall 17 0 Jom C Moser s Zone Diameter cm O C Wishaw s Corridor Modify Pool Settings Width 1 0 cm Modify Platforms Use Platform Width Add Platform Delete Platform On Demand Platform O Lock Settings Number of Start Sections 4 Rotate Start Sections e Use Start Section with Random 24 M
41. information has been entered click OK to return to the Editing Project screen The animals that were added will appear on the animals list as shown in Figure 5 17 Figure 5 17 Editing Project Screen with Animals Added Editing Project text File Edit View Animal Trial Help Project Name text Animal List imal Edit Animal Gender 8 14 0814 8 14 0824 8 14 0834 8 14 0844 Species 8 14 0854 Strain Birth Date Weight Edit Pool Platforms Day Trial Animal Platfor Durati Raise DwellT Dwell Includ MNoOTATEC NIC WIATECD MATE VVIACA TDACK ING MOT A e Ni WA T eR VAZ V JE DA K NM UL l A E La WA CR MALE V I D E U R AC CING MEN A VIT A DO MA Creating a Trial Template A template is a trial format that will be used for each animal To create a Trial Template screen click View Edit Templates The Trial Templates screen shown in Figure 5 18 will appear Click the Template Wizard button and the screen shown in Figure 5 19 will appear Figure 5 18 Trial Templates Screen gt Trial Templates File Edit view Template Trial Help Template Wizard Trial List Comments Raise Afte Dwell Time Grell Cont Include Ra Template Wizard Figure 5 19 Template Wizard Template Wizard Template Name Number of days 1 Number of trials per day 1 Total Number of Trials Start on Day 1 Ma
42. ion Complete Water Maze Install Wizard med ASSOCIATES ME Install Wizard Complete The Install Wizard has successfully installed Water Maze Click Finish to exit the wizard Y DIG 703 USB Driver Fire Wire Camera Driver Med Test Water Maze instrumentation and software for research Finish 54
43. it Pool Platforms Animal List Trial List Add Animal Add Animal Run T D Y Trial Animal Comm MaxS StartS Platfor Platfor Durati Raise Dwell Dwell Incdud A Gender 8 14 0814 v 8 14 0824 Y iit 8 14 0834 gt Vo i 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 Yes 12 200 aeie ERA v4 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 Yes 12 200 id Vv 1 1 8 14 0 Testing 120 3 platfor On De 2 000 30 000 2 000 Yes 12 200 rat C 1 2 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 m 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 Strain E 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 spraque daw E 1 2 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 3 progg Ja 1 2 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 Birth Date E 1 3 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 a 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 00 E 1 3 8 14 0 Testing 120 2 platfor OnDe 2 000 30 000 2 000 Yes 12 200 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 Weight E 1 3 8 14 0 Testing 120 2 platfor On De 2 000 30 000 2 000 Yes 12 200 Pp 1 4 8 14 0 Testing 120 2 platfor OnDe
44. ntis 06 13 08 13 34 01 Ready MED Associates 4602F_BL 41 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Automatically Recalculating the Animal s Position Refer to Figure 6 11 when completing the following steps Check the Recalculate Animals Position checkbox 1 Use the slider bar to locate the place in the video to begin retracking the animal 2 Click on the animal 3 then click the play button 4 When the video is complete click the Stop trial button 5 The animal s position should now be properly tracked Figure 6 11 Automatically Recalculating Animal s Position ta Start Water Maze 06100 fm Stalbans TempShar Water Maze 061008 C5 Testing x Start Time 13 34 01 06 13 06 Duration Latency to platform Distance Traveled cm Average Velocity 0 000 cmi s Gallagher s Proximity Show Swim Path Thigmotaxis Distance from pool wall 20 0 cm Moser s Zone Diameter 20 0 cm t s Corridor width 11 0 jcm Use Platfo Nidth Sections Time in Stark Section 4 0 0 Number of Sections 4 1 gt Rotation 180 Ise Start Locations Reanalyze Recalculate Animals Position BL M44 44 Ph hPL c0 00 000 00 33 400 x1 00 D isp test 06 13 06 13 34 01 C57 4 wmv ettings Load Settings Animal Comments Start Section Platform Position Start Date amp Time Duration Training Ready MED Associates 4602F_BL M
45. oject screen completed trials will be highlighted in yellow Once a trial has been completed the user can play back the video Also analysis settings can be modified and reports can be generated detailing the experiment data and the analysis results Figure 5 26 Main Screen with Trials Water Maze text File Edit View Export Trial Analysis Yideo Help E Stop Analysis x Start Time SSS Latency to platform Distance Traveled 0 000 lem Average Velocity 0 000 cms Gallagher s Proximity Show Swim Path C Thigmotaxis ii ff Distance from pool wall 0 0 Jem O Moser s Zone ed Diameter cm C Wishaw s Corridor ith 12 2 fem Use Platform Width Sections Time in Start Section 3 0 0 Number of Sections o Rotation o Use Start Locations Reanalyze Save Settings Load Settings Recalculate Animals Position b E ed gd pe Hb 00 00 000 00 00 000 x1 00 C Show All Trials Select Trials Video PIE Comments Start Section Platform Position Start Date amp Time Duration 8 14 0814 Testing 3 platform 1 8 14 0824 Testing platform 1 8 14 0834 Testing platform 1 8 14 0844 Testing platform 1 8 14 0854 Testing platform 1 Ready MED Associates 4602F_BL 36 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING CHAPTER 6 Opening an Existing Project TO open an existing project open the software application and the Select Project Wizard screen sho
46. ot be moved easily Once the pool support is leveled and in the correct location center the pool on top of it Next the floor insert should be placed inside the pool If the pool is equipped with an optional heater it will be under the floor insert The platforms should then be placed on top of the floor insert If an optional On Demand Platform is being used it should be connected to the bulkhead connector prior to filling the pool Refer to the On Demand Platform User s Manual for further information Figure 3 5 Exploded Diagram of Water Maze Hardware ENV 596 Fixed Platform ENV 595 On Demand Platform ENV 594 FI Floor Insert ENV 597 Heater ENV 594 Pool ENV 593 Pool Support MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Wiring the System 1 Using the included Firewire cable connect the Firewire camera to the Firewire card on the back of the computer Figure 3 6 Connect the Camera to the Firewire Card so Computer with Water Maze Video Tracking Software i g amp i i I FireWire FireWire Cable Camera 2 Using the included USB cable connect any available USB port on the computer to the USB port on the back of the DIG 703A USB Interface Figure 3 7 Connect the Computer to the USB Interface Computer with Water Maze Video Tracking Software OUTPUTS OUTPUTS 100 240 VAC SS anes peat 50 60 Hz gi FUSE 3A Back of the DIG 703A USB In
47. pool is equipped with a bulkhead connector for the On Demand Platform be sure that the On Demand Platform Cable or the bulkhead connector cover is applied to the bulkhead connector CHAPTER 4 User Interface Main Screen Menu Bar If the cursor is held over a menu item a description of its function will appear in the status bar This is the menu bar that is accessible from the Main Screen File Menu Figure 4 1 File Menu mis Edit View Export Trial Analysis Video Help Mew Open Select Project Wizard Exit Menu Item Description New Create new project Open Open existing project Select Project Open the Select Project Wizard Wizard screen Figure 5 1 Exit Close the application Edit Menu Figure 4 2 Edit Menu File A View Export Trial Analysis Video Help Pool Preferences Menu Item Description Open the Edit Pool Parameters Pool screen Figure 5 3 Open the Preferences screen Preferences Figure 5 14 For More Info Chapter 5 Starting a New Project Chapter 6 Opening an Existing Project Chapter 5 Starting a New Project and Chapter 6 Opening an Existing Project N A For More Info Chapter 5 Editing Pool Parameters Chapter 5 Setting Preferences View Menu Figure 4 3 View Menu File Edit WEVA Export Trial Analysis Video Help w Main Screen Edit Project Edit Templates Toolbars j Menu Item Description Main Screen Open the Main Screen Figure 5 26 Edit Project Open the Editing Project Sc
48. r Maze Version M Ma A E Copyright 2 2004 2008 MED Associates Portions utilize Microsoft Windows Media Technologies Copyright c 1999 2002 Microsoft Corporation All Rights Reserved Edit Project Menu Bar Most of the menu items on the Edit Project menu bar are the same as those on the Main Screen the only exceptions are the Animal and Trial menus shown below Animal Menu Figure 4 10 Animal Menu File Edit view Trial Help Menu Item Apply Template Add to Trials Add Edit Delete Trial Menu Description Apply a template to the selected animal Adds a new trial for the selected animal Add an animal Open the Edit Animal screen Delete the selected animal For More Info Chapter 5 Applying the Template to the Animals Chapter 5 Template Wizard Chapter 5 Adding Animals to Project Chapter 5 Adding Animals to Project The items on the Trial Menu allow the user to make changes to individual trials from the Trial Templates screen Figure 5 21 This menu can also be viewed by right clicking on a trial See Chapter 5 Creating a Trial Template for more information Figure 4 11 Trial Menu File Edit View Animal Help Modify Run Today Modify Day Modif Trial Modify Comments Modify Max Swim Duration Start Section j Platform Position j Modif Platform Duration Copy Selected Trial Delete Selected Trial Edit Templates Menu Bar Most of the menu items on the Trial Templates menu b
49. re in place using the included nuts and screw the arm onto the mounting bracket Figure 3 2 Orienting Drop Ceiling Camera Mounting Clips Figure 3 3 Installing the Camera Mount on a Drop Ceiling MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Leveling the Optional Pool Support The optional ENV 593 Pool Support is equipped with two bubble levels and height adjustable legs that allow it to be leveled quickly and easily Turn the handle on the height adjustable legs clockwise to lower their height and counterclockwise to raise them Adjust the legs until the bubble levels are both aligned properly NOTE The height of the legs should only be adjusted enough to level the pool support but not to raise and lower it s height Do not attempt to level the pool support when the pool is filled Figure 3 4 Optional Pool Support Leveling Features Bubble Levels MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Assembling Hardware The diagram shown in Figure 3 5 illustrates the order in which the hardware should be placed on the pool support After installing the camera mount in the desired location see the Installing the Camera Mount section of this manual and attaching the camera to it place the pool support in the desired location It may be necessary to open the software application and verify that the pool is oriented inside the camera field of view It is important to remember that once the pool is filled it cann
50. reen Figure 5 15 Edit Templates Open the Trial Templates Screen Figure 5 18 For More Info Chapter 5 Starting a Trial Chapter 5 Adding Animals to Project and Applying the Template to the Animals Chapter 5 Creating a Trial Template Export Menu Figure 4 4 Export Menu File Edit View ieee Trial Analysis Video Help Generate Reports Video Menu Item Description Opens the Select Trials for Reporting Screen and allows the user to generate reports using data from completed trials Figure 7 1 Generate Reports Opens the Export Options screen Figure Swim Path 7 2 and allows the user to export the swim path data Swim Path Exports a video of the swim path Video Video with Swim Path Exports a video of the video and the swim path For More Info Chapter 7 Generating Reports Chapter 7 Exporting Swim Path Chapter 7 Exporting Video Trial Menu Figure 4 5 Trial Menu File Edit View Export Analysis Video Help Show All Menu Item Description For More Info Start Starts the trial Chapter 5 Starting a Trial Stop Stops the trial Chapter 5 Starting a Trial h ll of th ial he Trial Li Show All ee a a iid On Chapter 5 Starting a Trial the Main Screen Rerun Rerun the trial Chapter 6 Rerunning a Trial Edit Animal h Editi he Animal ae lid Edit the animal s position during the trial E aes ey along TAE ANIAN Position Position Analysis Menu Figure 4 6 Analysis Menu
51. rm before the trial is considered complete Enter the amount of time that should pass before the On Demand platform is raised even if the animal hasn t been inside the include radius during the trial Enter the amount of time that the animal must spend inside the include radius before the platform is raised The dwell time must be consecutive seconds The dwell time will be cumulative seconds E g The dwell time is set to 2 seconds If the animal is inside the Include Radius four times for 0 5 second each time the On Demand platform will be raised Enter the radius within which an animal must dwell for a certain amount of time see Dwell Time to raise the On Demand Platform This radius is centered over the On Demand Platform These options only enabled if On Demand is selected from the Platform Type pulldown menu Once all of the necessary information has been entered click OK to return to the Trial Templates screen An example of a completed Template Wizard is shown in Figure 5 20 This screen would result in the Trial Template screen shown in Figure 5 21 Review the trial information To make corrections click the Template Wizard button again If it is correct click View Edit Project to return to the Editing Project screen To customize individual trials right click on the desired trial as shown in Figure 5 22 Or select the trial and open the Trial menu Figure 4 11 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING
52. side the Include Radius to raise the On Demand Platform The radius within which an animal must dwell for a certain amount of time see Dwell Time to raise the On Demand Platform A circle with a user defined radius drawn around the location of the platform in training trials that are analyzed during probe trials for platform crossings a k a pass throughs Occurs in a probe trial when the animal s path traverses the precise location where the escape platform was positioned during training Trial in which the platform has been removed following training to assess the animal s search strategy Number of sections that the pool is divided into This number is user defined and can be used during data analysis to determine how much time the animal spent in each section The tendency of the animal to swim around the edge of the pool Trial in which an animal is placed in the water maze and given a time to locate an escape platform User defined rectangular zone from the start position to the platform The sum of the distance from the platform at each second during the trial divided by the number of seconds i e average distance from the platform during the trial This is an average measure of total distance from the platform during the trial A correction procedure is used so that trial performance is relatively unbiased by the differences in distance to the goal from the various start locations at the perimeter of the pool
53. sting 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 2 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 3 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 4 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 5 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 6 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 7 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 8 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 9 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 2 10 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 1 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 2 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 3 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 4 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 5 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 6 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 7 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 8 Testing 120 2 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 9 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 3 10 Testing 120 4 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 1 Testing 120 3 platform 1 On Demand 2 000 30 000 2 000 Yes 12 200 4 pa Testing 120 Z platform 1 On D
54. sting Project start Water Maze 06100 Water Maze 061008 C57 Testing File Edit Yiew Export Trial Analysis Video Help ae Start Stop Start Time 13 27 38 06 13 08 Duration 00 17 800 Latency to platform 00 15 866 Distance Traveled 380 428 cm Average Velocity 23 503 cms Gallagher s Proximity 462 249 Show Swim Path C Thigmotaxis Distance from pool wall 5 0 Jem O Moser s Zone Diameter cm C Wishaw s Corridor 18 3 Width 11 0 Jem Use Platform Width C Sections Time in Start Section 4 11 9 Number of Sections Rotation Use Start Locations Save Settings Load Settings I Recalculate Animals Position b E 144 44 gt gt bb 00 00 000 00 17 800 1 00 D sp test 06 13 08 13_27_38 C57 1 wmv C Show All Trials x ME x Comments Start Section Platform Position Start Date amp Time Duration Training i Section 2 Atlantis 06 13 08 13 27 38 Ready MED Associates A602f_BL 38 Video Playback Controls Use the buttons shown in Figure 6 5 to control a file s video playback To advance one frame at a time click on the slider bar and the slider will turn from green to yellow as shown The left and right arrow keys on the computer keyboard may now be used to advance the video one frame at a time The Page Up and Page Down keys on the computer keyboard can be used to advance once second at a time The video playback sp
55. terface MED ASSOCIATES INC WATER MAZE VIDEO TRACKING 3 Connect the SG 205 HD Water Resistant Pushbutton Switch to the appropriate connector on the SG 597J Junction Box Using the SG 222 cable connect INPUT 1 on the back of the DIG 703A USB Interface to the appropriate connector on the SG 597J Figure 3 8 Connect the Remote to Input 1 Back of the DIG 703A USB Interface 100 240 VAC 50 60 Hz SG 222 Cable SG 205 HD Water SG 597 ISSOCITTRE ING Resistant Junction Pushbutton Box Switch SG 597 Handheld Pushbutton Junction Box 4 Connect the DIG 703A USB Interface to a GFCI wall outlet Figure 3 9 Connect the Interface to GFCI Wall Outlet 100 240 VAC 50 60 Hz AN GFCI Wall Outlet FUSE 3A Back of the DIG 703A USB Interface 5 If an On Demand Platform is being used carefully read the On Demand Platform User s Manual for setup and detailed wiring instructions 1 Filling the Pool Prior to filling the tank be sure the following tasks have been completed The camera is mounted in the correct location and is in focus see the Adjusting Camera Zoom Focus and Aperture section of Appendix A The Optional Pool Support is level and stable The Optional Floor Insert is installed The platform fixed or On Demand is in the pool The pool is in the desired location and inside the camera s field of view The pool drain valve is closed If the
56. wn in Figure 6 1 will appear Clicking File Select Project Wizard will also access this screen Recently opened projects will be listed on the Select Project Wizard screen other projects can be accessed by clicking the Load Project button which will open the Open File screen shown in Figure 6 2 Figure 6 1 Select Project Wizard Select Project Wizard ES New Project Load Project 0610085 CS Testing Water Maze text Water Maze Project Open this wizard automatically on startup Highlight the desired project and click Open The Select Project Wizard will appear again as shown in Figure 6 3 Figure 6 2 Open File Look in y Water Maze Data Y pe file E best Water _Maze_ Project 1 4 text Water_Maze Project My Recent Documents Es Desktop My Documents y My Computer Me File name test Water Maze Project w My Network Files of type Project Files Water Maze Project we 3 7 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING The desired project will now appear on the Select Project Wizard list Highlight the desired project and click OK The main screen will appear as shown in Figure 6 4 Figure 6 3 Select Project Wizard with Desired Project Select Project Wizard E Mew Project Load Project Open this wizard automatically on startup Highlight a trial to display the corresponding Analysis data and settings Figure 6 4 Main Screen with Opened Exi
57. x Swim Duration sec 120 Trial type Start Section Random Platform Position On Demand Platform Platform Type Standard Platform Duration sec 2 000 The following information must be entered into the Template Wizard screen Template Name User defined Template Name Number of days Number of days that the project will run Number of trials per day Number of trials per day per animal Start on Day Enter the day to begin this template Max Swim Duration sec Trial Type Start Section Platform Position Platform Type Platform Duration sec Raise After sec Dwell Time sec Continuous Cumulative Include Radius cm Enter the maximum amount of time that the animal can be in the pool Enter the type of trial for example Training or Testing Select the Start Section 1 2 3 or 4 or Random in which the animal will be placed at the beginning of the trial Random selection will randomly select from the sections enabled on the Edit Pool Parameters screen Select the platform that is being used This list will display the platforms added on the Edit Pool Parameters screen Select the type of platform from the pulldown menu The options are Standard On Demand or Null The Null setting should be used when the platform is not being used in the pool but its location should still be defined in software Enter the amount of time that the animal must remain on the platfo
58. y on startup Enter the desired file name in the File name field and click Create The screen Edit Pool Parameters screen shown in Figure 5 3 will appear Figure 5 2 New Project Screen New Project My Recent Documents Fie name My Network Files of type Project Files Water Maze_Project ha 20 MED ASSOCIATES INC WATER MAZE VIDEO TRACKING Editing Pool Parameters Modify Pool Settings From the screen shown in Figure 5 3 click Modify Pool Settings to enable the pool settings options The screen will appear as shown in Figure 5 4 Figure 5 3 Edit Pool Parameters Screen with Settings Locked Edit Pool Parameters Pool Diameter cm 120 650 PoS C Moser s Zone Diameter cm Lock Settings C Wishaw s Corridor Modify Pool Settings Cielo y width 10 Jen O Modify Platforms Use Platform Width Add Platform Delete Platform Number of Start Sections Rotate Start Sections 9 Use Start Section y 1 with Random M2 Sections M3 Vi The pool boundary is user defined and is indicated by the blue bounding circle This bounding circle should be adjusted until it is just inside the edges of the water surface Adjust the pool boundary by placing the cursor over a handle until it turns to a ie then clicking and dragging The circle can be rotated by placing the cursor close to a handle until it changes to a a It can be moved by placing the cursor inside the circle until it chan

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