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ÄKTA pure Operating Instructions

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1. WARNING Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect In this section This section contains the following subsections Section See page 4 2 1 Unpack the instrument 61 4 2 2 Install the computer equipment 67 4 2 3 Connect system units 68 4 2 4 Prepare waste tubing 71 4 2 5 Prepare the pump rinsing system 74 4 2 6 Start the instrument and the computer 77 60 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 1 Unpack the instrument 4 2 1 Unpack the instrument Introduction This section describes how to unpack the AKTA pure instrument and how to lift the in strument onto the bench CAUTION Heavy object Use proper lifting equipment or use two or more persons when moving the instrument All lifting and moving must be performed in accordance with local regulations Lift the instrument onto the bench by hand Follow the instruction below to remove the transport fixations and lift the instrument onto the bench Step Action l Cut and remove the plastic straps KTA pure Operating Instructions 29 0229 97 AE 61 4 Installation 4 2 Hardware installation 4 2 1 Unpack the instrument Step Action 2 Lift off and remove the lid and protective foam r 3 Check the contents in the Buffer tray and lift off the
2. NOTICE The maximum level of the waste vessel must be lower than the bottom of the KTA pure instrument KTA pure Operating Instructions 29 0229 97 AE 71 4 Installation 4 2 Hardware installation 4 2 4 Prepare waste tubing Step Action 1 Insert the waste tubing from all installed modules in a vessel 2 Make sure that the tubing is securely fastened to the KTA pure instrument e Fasten waste tubing from the valves with the clips on the front of the system e Fasten waste tubing from the Buffer tray with the clips on the rear of the system 72 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 4 Prepare waste tubing Step Action 3 Cut the waste tubing to appropriate length It is important that the tubing is not bent and will not be submerged in liquid during the run Note If the tubing is too short replace it with new tubing Do not lengthen the tubing as this might cause obstruction of the tubing 4 Fasten all waste tubing securely to the waste vessel AKTA pure Operating Instructions 29 0229 97 AE 73 4 Installation 4 2 Hardware installation 4 2 5 Prepare the pump rinsing system 4 2 5 Prepare the pump rinsing system Illustration of the pump piston rinsing system The pump piston rinsing system protects the seal that prevents leakage between the pump chamber and the drive mechanism of the pump The illustration below shows
3. AKTA pure Operating Instructions 29 0229 97 AE 89 4 Installation 4 6 Performance test 46 Performance test Before taking the AKTA pure instrument into use run a performance test to check the function of the equipment See AKTA pure User Manual for further instructions 90 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 7 Activate Power save 47 Activate Power save Introduction AKTA pure has a power save mode The instrument enters Power save after having been in the Ready state for a set period of time The system enters the Ready state when a method run a method queue or a manual run ends Enable power save To enable Power save a system must be connected and in state Ready Follow the instructions below to activate Power save Step Action 1 In the System Control module on the System menu click Settings Result The System Settings dialog opens e Click Advanced and e select Power save r System Settings kta Pure Instructions Parameters for Power save Mode Off On Time 10 1440 60 min Powersave Instrument contro panel Pressure control parameters Constant pressure flow parameters Max flow during valve tum ae 5 e Click On in the Mode field and e type the number of minutes in the Time field Note This is the time the instrument will be in state Ready before power save mode is entered AKTA pure Operatin
4. 2 Safety instructions 2 1 Safety precautions CAUTION Reversed Phase Chromatography RPC runs with 100 acetoni trile in AKTA pure Always replace the PEEK tubing between the used system pump and the pump pressure monitor with orange PEEK tubing i d 0 5 mm before running RPC with 100 acetonitrile The tubing to be replaced is green for 25 ml min systems and beige for 150 ml min systems Set the System pressure alarm to 10 MPa for 25 ml min systems CAUTION Moving tubes with flammable liquids Move tubes filled with flammable liquids carefully to prevent spillages that may come in contact with an ignition source Personal protection CAUTION Always use appropriate personal protective equipment during op eration and maintenance of AKTA pure CAUTION Hazardous substances When using hazardous chemical and bio logical agents take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation maintenance and decommissioning of the equipment CAUTION High pressure AKTA pure operates under high pressure Wear protective glasses at all times AKTA pure Operating Instructions 29 0229 97 AE 15 2 Safety instructions 2 1 Safety precautions Installing and moving the instrument 16 CAUTION Cut injuries The tubing cutter is very sharp and must be handl
5. Chemical environment See AKTA pure User Manual Section 8 2 Chemical resistance guide on page 130 128 AKTA pure Operating Instructions 29 0229 97 AE Operating ranges Parameter Operating temperature range 8 Reference information 8 1 System specifications 4 C to 35 C Relative humidity Equipment noise level Equipment AKTA pure instrument 20 to 95 non condensing Acoustic noise level KTA pure Operating Instructions 29 0229 97 AE lt 60dBA 129 8 Reference information 8 2 Chemical resistance guide 8 2 Chemical resistance guide Introduction This section specifies the chemical resistance of AKTA pure to some of the most com monly used chemicals in liquid chromatography Biocompatibility AKTA pure is designed for maximum biocompatibility with biochemically inert flow paths constructed mainly from titanium PEEK and highly resistant fluoropolymers and fluoroe lastomers Titanium is used as far as possible to minimize contribution of potentially deactivating metal ions such as iron nickel and chromium There is no standard stainless steel in the flow path Plastics and rubber materials are selected to avoid leakage of monomers plasticizers or other additives Cleaning chemicals Strong cleaning works well with 2 M sodium hydroxide 70 acetic acid or the alcohols methanol ethanol and isopropyl alcohol Complete system cleaning using 1 M hydrochlo ric acid should b
6. 1 3 Regulatory information Introduction This section lists the directives and standards that are fulfilled by AKTA pure Manufacturing information The table below summarizes the required manufacturing information For further infor mation see the EU Declaration of Conformity DoC document Requirement Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Conformity with EU Directives This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards Directive 2006 42 EC Machinery Directive MD 2004 108 EC Electromagnetic Compatibility EMC Directive 2006 95 EC Low Voltage Directive LVD 1999 5 EC Radio Equipment and Telecommunications Terminal Equipment R amp TTE Directive International standards This product fulfills the requirements of the following standards Standard Description EN ISO 12100 Safety of machinery General princi EN ISO standard is har ples for design Risk assessmentand monized with EU direc risk reduction tive 2006 42 EC KTA pure Operating Instructions 29 0229 97 AE 9 1 Introduction 1 3 Regulatory information Standard Description EN IEC 61010 1 Safety requirements for electrical EN standard is harmo UL 61010 1 equipment for measurement control nized with EU directive CAN CSA C22 2 and laboratory use 2006 95 EC
7. 124 Manufacturing information 9 N Node ID change Node ID 137 check Node ID 137 description 135 Notes and tips 8 O Operating conditions 56 Outlet tubing prepare 98 P pH monitor KTA pure Operating Instructions 29 0229 97 AE storage of the pH elec trode 121 Power requirements 58 Power save 91 Prepare the system connect column 100 prime inlets 82 99 purge system pumps 82 99 Start UNICORN 79 waste tubing 71 Pressure alarm set 105 Prime inlet tubing B 82 Process picture 47 118 Pump piston rinsing system illustration 74 prime 75 Pumps purge system pumps 82 99 R Recycling disposal 29 Recycling procedures 29 Reference information 126 chemical resistance guide 130 recycling 29 Regulatory compliance 11 Regulatory information 9 international standards 9 Room requirements introduction 53 laboratory bench 54 Run after run procedures 119 cold room temperature 108 start 116 S Safety AKTA pure Operating Instructions 29 0229 97 AE Index notices 8 Safety precautions emergency procedures 26 introduction 13 labels 23 24 personal protection 92 123 Site preparation 50 Software overview 45 software modules 45 Space requirements 54 Storage 52 column 120 OH electrode 121 system 120 System cleaning 119 System Control module description 46 icons 48 process picture 47 System preparation before preparation 94 System storage 120 T Typograp
8. 5 5 Pressure alarms Introduction The columns can be protected by two different types of pressure alarms e The pre column pressure alarm protects the column hardware e The delta column pressure alarm only available when V9 C or V9H C is installed protects the column media Column valves V9 C and V9H C have built in pressure sensors that automatically measure the pre column and delta column pressure If Column valve V9 C or V9H C is not used column is connected without a Column valve or to Column valve V9 Cs or V9H Cs the pre column pressure is calculated from the system pressure and tubing dimensions See the instructions below to set the pressure alarm for the column to be used in the run and if applicable to set the parameters for the tubing dimensions Set tubing dimension parameters to calculate pre column pressure For instruments where there is no pre column pressure sensor i e the column is con nected without a Column valve or to Column valve V9 Cs or V9H Cs the pre column pressure is calculated from the system pressure and tubing dimensions Follow the in structions below to set the tubing dimension parameters Step Action 1 Select System Settings in the System Control module Result The System Settings dialog opens AKTA pure Operating Instructions 29 0229 97 AE 105 5 Prepare the system for a run 5 5 Pressure alarms Step Action e Select Tubing and Delay Volumes and e select Tubing In
9. No 61010 1 EN IEC 61326 1 Electrical equipment for measure EN standard is harmo Emission accord Ment control and laboratory use nized with EU directive ing to CISPR 11 EMC requirements 2004 108 EC Group 1 class A ETSI EN 301 489 3 Electromagnetic compatibility and EN standard is harmo Radio spectrum Matters ERM Elec nized with EU directives troMagnetic Compatibility EMC 1999 5 EC standard for radio equipment and Services ETSI EN 300 330 2 Electromagnetic compatibility and EN standard is harmo Radio spectrum Matters ERM Short nized with EU directive Range Devices SRD Radio equip 1999 5 EC ment in the frequency range 9 kHz to 25 MHz and inductive loop sys tems in the frequency range 9 kHz to 30 MHz FCC compliance This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation Note The user is cautioned that any changes or modifications not expressly approved by GE could void the user s authority to operate the equipment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide rea sonable protection against harmful interference when the equipment is operated in a
10. Note It is recommended to replace the mixer sealing ring with the highly resistant O ring code no 29 0113 26 if the system is to be exposed to organic solvents or high concentrations of organic acids such as acetic acid and formic acid for a longer period of time Salts and additives for hydrophobic interaction chromatography HIC Continuous use Chemical Concentra CAS no EC no tion Ammonium chloride 12125 02 9 235 186 4 Ammonium sulfate 7783 20 2 231 984 1 Ethylene glycol 107 21 1 203 473 3 Glycerol 56 81 5 200 289 5 AKTA pure Operating Instructions 29 0229 97 AE 133 8 Reference information 8 2 Chemical resistance guide Reducing agents and other additives Continuous use Chemical Arginine Concentra tion CAS no EC no 74 79 3 200 811 1 Benzyl alcohol 100 51 6 202 859 9 Dithioerythritol DTE 3483 12 3 222 468 7 Dithiothreitol DTT 3483 12 3 222 468 7 Ethylenediaminetetraacetic acid EDTA 60 00 4 200 449 4 Mercaptoethanol 37482 11 4 253 523 3 Potassium chloride Other substances Chemical Acetone Concentra tion 7447 40 7 231 211 8 CAS no EC no 67 64 1 200 662 2 Ammonia Dimethyl sulphoxide DMSO 7664 41 7 231 635 3 67 68 5 200 664 3 Ethanol for long term stor age Phosphoric acid 75 08 1 200 837 3 7664 38 2 231 633 2 AKTA pure Operating Instructions 29 0229 97 A
11. 120 KTA pure Operating Instructions 29 0229 97 AE 6 Runa method 6 5 After run procedures In the following situations in order to increase the lifetime of the pH electrode use the By pass position and store the electrode in storage solution inside the pH flow cell e pH monitoring is not needed during the run e Organic solutions are used e Extremely acidic or extremely basic solutions are used For further information on how to prepare the pH electrode for storage refer to AKTA pure System Handbook Log off or exit UNICORN Follow the instructions to log off or exit UNICORN This can be performed from any of the UNICORN modules If you want to log off UNICORN on the File menu click Log off g Log off Eric Exit UNICORN Result All open UNICORN modules close and the Log On dialog opens exit UNICORN on the File menu clickExit UNICORN Logoff Eric Exit ONITORM Result All open UNICORN modules close Note If an edited method or result is open and not saved when you try to exit or log off UNICORN you will see a warning Click Yes to save No to exit without saving or Cancel to stay logged on AKTA pure Operating Instructions 29 0229 97 AE 121 6 Runamethod 6 5 After run procedures Shut down the instrument Switch off the instrument by pressing the power switch to the O position N A gu py 122 KTA pure Operating Instructions 29 0229 97 AE 7 Maintenance 7 Main
12. Name Default Domain Access Access ToEverything X Group Start V Administration V System Control T Method Editor Evaluation o Cancel Result The selected UNICORN modules open KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 4 Start UNICORN and connect to system Connect to system Follow the instructions to connect the instrument to UNICORN Step Action 1 In the System Control module click the Connect to Systems button System name Control View C E System E System4 D E Systems C E System C E System 2 In the Connect to Systems dialog e Select a system check box e Click Control for that system e Click OK Result The selected instrument can now be controlled by the software Tip If UNICORN is unable to connect to the selected instrument see Chapter Troubleshooting in KTA pure User Manual KTA pure Operating Instructions 29 0229 97 AE 81 4 Installation 4 5 Prime inlets and purge pump heads 45 Prime inlets and purge pump heads Introduction Before usage of the system pumps it is important to e Prime the inlets fill the buffer inlets with liquid e Purge the system pumps remove air from the pump heads Note Note that the procedures described in this section may have to be adapted if your system configuration differs from the one described in this manual Overview The procedure consists of the following stages Stage Descriptio
13. Operating Instructions 29 0229 97 AE 2 Safety instructions Meaning The system complies with applicable requirements for Australia and New Zealand 2 2 Labels The system complies with applicable European direc tives This symbol indicates that KTA pure has been certified by a Nationally Recognized Testing Laboratory NRTL NRTL means an organization which is recognized by the US Occupational Safety and Health Administration OSHA as meeting the legal requirements of Title 29 of the Code of Federal Regulations 29 CFR Part 1910 7 This symbol indicates that electrical and electronic equipment must not be disposed of as unsorted mu nicipal waste and must be collected separately Please contact an authorized representative of the manufac turer for information concerning the decommissioning of equipment AKTA pure Operating Instructions 29 0229 97 AE This symbol indicates that the product might contain hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Sub stances in Electronic Information Products 25 2 Safety instructions 2 3 Emergency procedures 2 5 Emergency procedures Introduction This section describes how to perform an emergency shutdown of the AKTA pure instru ment including connected equipment This section also describes the results in the event of power failure or network interr
14. System A ee Eric Methods Al Method Systema Method Systema Results 2 Select the method to run and click the Run button eal Result The Start Protocol dialog opens 3 Step through the displayed pages in the Start Protocol add requested input and make appropriate changes if necessary Click Next 116 AKTA pure Operating Instructions 29 0229 97 AE 6 Runa method 6 3 Start a method run Step Action 4 Click Start on the last page of the Start Protocol Result e lf column logging was included during installation of UNICORN and a column type was selected at method creation the Select Columns dialog opens For further information on column handling please refer to UNICORN Method Manual and UNICORN System Control Manual e If column logging was not chosen during installation of UNICORN and or no column type was selected at method creation the run starts directly AKTA pure Operating Instructions 29 0229 97 AE 117 6 Runa method 6 4 Monitor the run 6 4 Monitor the run Introduction You may follow the on going method run in the System Control module The current system status is shown in the System state panel in the Run Data pane For example it may state Run Wash or Hold See Section 3 2 2 System Control module on page 46 for information about the data shown in System Control during a run the layout of the module and the procedure to customize the view of the different panes Process pi
15. V9 C 1 For other parts of the system up to 1 M HCl is acceptable for short periods of use See Cleaning chemicals on page 130 Solubilization and denaturing agents Continuous use as additives in separation and purification methods Chemical Concentra CAS no EC no tion Guanidinium hydrochloride 50 01 1 200 002 3 Sodium dodecyl sulfate SDS 151 21 3 205 788 1 TRITON X 100 9002 93 1 Tween 20 9005 64 5 500 018 3 AKTA pure Operating Instructions 29 0229 97 AE 8 Reference information 8 2 Chemical resistance guide Chemicals used in reversed phase chromatography RPC Continuous use Chemical Concentra CAS no EC no tion Acetonitrile 1 100 75 05 8 200 835 2 Acetonitrile Tetrahydrofu 85 15 109 99 9 203 726 8 1 ran Acetonitrile water Trifluo Max 0 2 N A roacetic acid TFA 2 TFA Ethanol 100 75 08 1 200 837 3 lsopropanol 100 67 63 0 200 661 7 Methanol 100 74 93 1 200 659 6 Water organic mobile Max 5 phase formic acid formic acid 1 Organic solvents can penetrate weaknesses in PEEK tubing walls more easily than water based buffers Special care should therefore be taken with prolonged use of organic solvents close to pressure limits Note Quaternary valve is not resistant Depending on pressure tubing between pump head and pressure monitor needs to be changed See AKTA pure User Manual for more information 2 Mobile phase system
16. chosen column type However when running manual runs you have to set the pressure limits yourself Also to protect the column media special settings are needed See Sec tion 5 5 Pressure alarms on page 105 for more information on pressure alarms Note Do not overtighten when connecting columns Overtightening might rupture the connectors or squeeze the tubing and thereby result in high back pressure Note If no column valve is used remove the column from the system before running a system wash The pressure during a system wash may become too high for the column 100 KTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 4 Connect a column Attach a column holder and connect a column Follow the instructions below to connect a column to the instrument Always use a column holder If a column valve is used connect the column to the appropriate A and B ports on the valve If no column valve is used connect the column directly to the flow path tubing Use appropriate tubing and connectors The instructions below show a system configured with Column valve V9 Cs Step Action i Attach an appropriate column holder to the rail on the instrument 2 Attach the column to the column holder 3 Connect a suitable tubing to a Column valve port in this example port 1A 4 Open the System Control module AKTA pure Operating Instructions 29 0229 97 AE 101 5 Prepare the system for a run 5 4 Con
17. commercial environment This equipment generates uses and can radiate radio frequen cy energy and if not installed and used in accordance with the Instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be re quired to correct the interference at his own expense 10 AKTA pure Operating Instructions 29 0229 97 AE 1 Introduction 1 3 Regulatory information Environmental conformity This product complies with the following environmental regulations Requirement 2011 65 EU Restriction of Hazardous Substances ROHS Directive 2012 19 EU Waste Electrical and Electronic Equipment WEEE Directive Regulation EC No Registration Evaluation Authorization and restriction of 1907 2006 CHemicals REACH ACPEIP Administration on the Control of Pollution Caused by Elec tronic Information Products China Restriction of Hazardous Substances ROHS CE marking The CE marking and the corresponding EU Declaration of Conformity is valid for the in strument when it is e used asa stand alone unit or e connected to other products recommended or described in the user documentation and e used in the same state as it was delivered from GE except for alterations described in the user documentation Regulatory compliance of connected equipment Any equipment connected to AKTA pure
18. for transportation up to 90 atmospheric humidity at 40 C for 48 hrs The following equipment is recommended for handling the delivery boxes Equipment Pallet mover Specifications Suitable for a lightweight pallet 80 x 100 cm Cart for transporting the instru ment to the lab 52 Dimensioned to accommodate the size and weight of the instrument KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 1 Site preparation 4 1 2 Room requirements 4 1 2 Room requirements Introduction This section describes the requirements for the transportation route and the room where the AKTA pure instrument is placed WARNING Protective ground The AKTA pure instrument must always be connected to a grounded power outlet WARNING Only use grounded power cords delivered or approved by GE WARNING Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect CAUTION Explosion hazard To avoid building up an explosive atmosphere when using flammable liquids make sure that the room ventilation meets the local requirements gt gt gt gt Transportation route Doors corridors and elevators must have a minimum width of 65 cm to allow for trans porting the instrument Allow additional space for moving around corners KTA pure Operating Instructi
19. network card of the computer and a network wall outlet Note If the computer has not been supplied by GE and if network configuration is to be used see Administration and Technical Manual for further information on network settings 70 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 4 Prepare waste tubing 4 2 4 Prepare waste tubing The table below lists the waste tubing of the instrument and where it is located Make sure that the waste tubing is connected to the correct positions on the modules Module Tubing connections Location of tubing Injection valve Waste portsW1andW2 Front of the KTA pure instru ment pH valve optional Waste port W3 Front of the KTA pure instru ment Outlet valve optional Waste port W Front of the KTA pure instru ment Buffer tray Rescue Drainage hole of the Rear of the KTA pure instru drainage Buffer tray ment Instructions Follow the instructions below to prepare the waste tubing CAUTION Fasten the waste tubing During operation at high pressure the KTA pure instrument may release bursts of liquid in the waste tubing Securely fasten all waste tubing to the KTA pure instrument and to the waste vessel CAUTION Make sure that the waste vessel will hold all the produced volume of the run For KTA pure a suitable waste vessel should typically have a volume of 2 to 10 liters
20. packages from the tray 4 Lift off the cardboard hood and remove the protecting material from the instrument anong 8 6 Your KTA pure 600 0 pt raves ama z SR pe Oh ne pa 62 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 1 Unpack the instrument Step Action 5 Lift off the tray on the wet side on the front of the instrument to access the instrument handles AKTA pure Operating Instructions 29 0229 97 AE 63 4 Installation 4 2 Hardware installation 4 2 1 Unpack the instrument Step Action 6 Prepare for lifting Use two or more persons and grip the instrument from the front from the back or from either side only one side is shown below 64 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 1 Unpack the instrument Step Action 7 Lift the instrument over the foam attached to the plywood board and pull away the board from under the instrument 8 Dispose of the packaging material in accordance with local regulations Note The instrument flow path is filled with 50 ethanol at delivery Accessories packages The illustration below shows the accessories packages placed in the Buffer tray at delivery 1 2 5 Description 1 Accessories box KTA pure Operating Instructions 29 0229 97 AE 65 4 Installation 4 2 Hardware installation 4 2 1 Unpack the instrument Part D
21. sasssa nnn en ECE ee ae eae ene ree VARER 4 1 3 Site environment sssnsrsno niti innn SNR ase N ESAO NARSES 4 1 4 FONC FECT EMENI seana TA TAAA EOE 4l CCN IS SONA ecserin aE eat ace iedae 4 2 1 Unpack TNC INStrUMEN scassccescecasscocicitecerccsttecseustastenancdehvdsorertatcoeacosteveenwncnaemeemteanasceiiedns 42 2 Install the computer COG OIE sass secs ces et cerescctvccccectviess cae pestsues ates tet ciectcbenverteteerenceheiauee 4 2 5 CONNECT VANT UNE sisi ae EEEE EEEE ae eee eee renee ee 4 2 4 Prepare waste TUONG orrerarseissniio wida n neriie A E 4 2 5 Prepare the pump rinsing SV SUC sirni 4 2 6 Start the instrument and the COMPUTE s ssssssssssssssssssssrrirrssssssssssssrsrrssrrrrrsssssssssrrrrrnn 43 Goftware SIU 6 18 9 emer Omer enna ne as ne ot ET oO SNES ea Oe Oe EET 44 Start UNICORN and connect tO SYSTEM oecececcccsssessssssesssesssecssecssscsscsssssssssssecssscssecssecsseceseessueeses 45 Prime inlets and purge pump heddS scsasesssceccicaszcsvessnccsssoiscsntssnnsteutdenseceeedsadhiuocsdedvieasocpacttiuelatoours A0 PEnmMANCE TES E uun E E Ar 1810 1 Seg POWE GOVE seinor eR RUT TP Fee EOE ne TOES En eee 5 Prepare the System fOr a run ccsccsscesscessscsssecsccssccssseessscssscesscesseessscsssecseeees Sob Bege VOU PRG O iS tie Sy SUS UV eperra Se P VO CIA eA N S saseeisceacee torent 5 5 PRIME inlets and purge pump headS sarracciiscccttsctnvavedccsscesonnesnsboesbbnteosudsedseetveonsaveuebb
22. should meet the safety requirements of EN IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked AKTA pure Operating Instructions 29 0229 97 AE 11 2 Safety instructions 2 Safety instructions About this chapter This chapter describes safety precautions safety labels and emergency shutdown pro cedures for AKTA pure Decommissioning information is also described In this chapter This chapter contains the following sections Section See page 2 1 Safety precautions 13 2 2 Labels aS 2 3 Emergency procedures 26 2 4 Recycling information 29 2 5 Declaration of Hazardous Substances DoHS 30 12 KTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 1 Safety precautions 2 1 Safety precautions Introduction The AKTA pure instrument is powered by mains voltage and handles pressurized liquids that may be hazardous Before installing operating or maintaining the system you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injuries or damage to the equipment The safety precautions in this section are grouped into the following categories General precautions Flammable liquids Personal protection Installing and moving the instrument System operation Maintenance Some of the safety precautions in this chapter concern modules or situations that are described in other manuals General pre
23. than the max pre column pressure hardware To protect the media if a delta column pressure measurement is not available that is when column valve V9 C or V9H C is not used the pre column pressure alarm must be manually set to the value in the column list that is the lowest of the max pre column pressure and the max delta column pressure Delta column pressure alarms If column valve V9 C or V9H C is installed the delta column pressure will be measured but the alarm must be set manually if needed Set pressure alarms Pressure alarm limits may be set manually in System Control The example below de scribes how to set the high pressure limit for the column Other alarms are set in a cor responding way Step Action 1 Select Manual Execute Manual Instructions in the System Control module Result The Manual instructions dialog opens 2 e Select Alarms and e select Alarm pre column pressure Manual instructions System 1 Selected column type _ L Select Instructions E E E eee Instruction execution list Paramete e cy sans A Mode k otes Disabled Enabled 5 Fraction collection High alarm 0 02 20 00 pane i 0 50 MPa Low alarm 0 00 20 00 Alarm conductivity 0 30 2 MPa Wash settings Watch parameters Advanced Save result as Auto update of parameters during run 3 Select Enabled in the Mode field e Type the high pressure limit in the High alarm field e Click Execu
24. to 6 for a valve module switch A is set to 0 and switch B is set to 6 3 Check the Node ID and compare it with the listed Node IDs in the tables above 4 To change the Node ID use a screw driver to set the arrows of the switches to the desired number 5 Re install the module in the instrument if applicable AKTA pure Operating Instructions 29 0229 97 AE 137 Index Index A AKTA pure illustrations 34 Apply sample 113 sample loop 113 C CE Conformity 9 CE marking 11 Cleaning column 120 system 119 Cold cabinet precautions 108 Cold room temperature 108 Column attach column holder 101 cleaning 120 connect column 100 pressure alarm 105 Storage 120 Connector ports placement 69 Connect system units 68 D Delivery box instrument 51 Dimensions instrument 55 E Emergency procedures emergency shutdown 26 power failure 27 F FCC compliance 10 Flow path illustration 96 prepare 95 Inlet tubing prime inlets 82 99 prime inlet tubing B 82 Installation hardware 60 performance test 90 prepare waste tubing 71 prime and purge pumps 82 site preparation 50 software 78 Instrument control panel 40 status indications 41 Instrument overview 33 exterior design 33 Instrument configura tions 33 Instrument control panel 40 main parts 34 modules 35 operating ranges 33 L Log on UNICORN 79 M Maintenance program 123 periodic 123 when required
25. 29 0182 26 29 0182 27 29 0182 28 29 0113 61 29 0466 65 29 0466 94 29 0466 97 0 RTRZAS AS MAE AaB A at PY BS BS ESJ T113 63 2006 Pr EE AY BR BE RAP x RURZASASWASYEZRAYR HRMS A SB BiB SI 711363 2006 ew 7E BY BR Be BES We Ze PIT Sl RHE A 2c 7h BY PT ER 1S BY aE 0 Indicates that this toxic or hazardous substance contained in all of the homogeneous ma terials for this part is below the limit requirement in SJ T11363 2006 x Indicates that this toxic or hazardous substance contained in at least one of the homoge neous materials used for this part is above the limit requirement in SJ T11363 2006 Data listed in the table represents best information available at the time of publication KTA pure Operating Instructions 29 0229 97 AE 31 3 The instrument and software 3 The instrument and software About this chapter This chapter gives an overview of AKTA pure instrument software and accessories In this chapter This chapter contains the following sections Section See page 3 1 AKTA pure instrument overview 33 3 2 UNICORN software 44 32 KTA pure Operating Instructions 29 0229 97 AE 3 The instrument and software 3 1 AKTA pure instrument overview 3 1 KTA pure instrument overview Introduction This section shows an overview of the AKTA pure instrument Technical details about the instrument and the individual modules are found in AKTA pure User Manual Exterior design AKTA pure has a modular
26. 8 miimn O35 MPa 031M f a 0 10 20 2 20 45 70 tem flow 5 998 53 0 prec 0 311 0 551 ma 37 12 7 83 fow 0 000 m Loop vent cO s pets Manualload By pass Al A Van Nene stat a aN C 14 p mmm M mmm Sy F 1 uy mmm Cond mmm Ostrictor oe Frac 6 Sia mw SP 4 at m DAP J MehodRun Block Linear gradient Nowstch Connection Connected in view Controlled by Default HCE LI27AV Note On the View menu click Run Log to open the Run Log pane which presents all registered actions AKTA pure Operating Instructions 29 0229 97 AE 3 The instrument and software 3 2 UNICORN software 3 2 2 System Control module Process picture The Process Picture displays the current flow path run parameters and real time data from monitors during a run It also allows manual interactions with the system Color indication is applied in the Process Picture as is shown in the following illustration and described in the following table system flow 5 998 mi m 53 0 se Prec 0 31 mee 0551 ma 37 12 ms em pH 7 83 Sample flow 0 000 mi m Or Deltac 0 13 mPa Manual load By pass ra mmm A1 aa A E wl pH vaive utiet Fra rN FD 14 P m M a SyP F 1 Uv mE Cond m inta mmm Frac Current A raction ans umn Indication Open flow path with flow Closed flow path or an open path without flow Syringe port in loop open for manual injection Modules without a fixed place in the system are
27. AKTA pure Operating Instructions Original instructions Page intentionally left blank Table of Contents Table of Contents TU cacteatterecieneearinsi eiecie een ieienentneennni we ADO a 1 9 0 EA RA T Pe evr nresny perry i Mapornant I SS WIFI OMEN Cl NOU eaea AE Me REJULO ROMA MC ND essee i aia E aae 2 Sdlety INSTrUCTIONS sic caiprcenenn sts teaceennaertnsecsnstorcacpentan cate dencaea abner Pa 2 16168 01010 10 9 lt lt arene emanate nnn nae ene Pe Ca TON een CDR mee ene e AO neon a on OPO On nT ETS PnP TaN ee 25 Emergency ProcedUlES sgean ene mre A mT ere ea eee oe ear ee 24 Recycing IVA OME OV acetic cece sat eaccas cnet ect E eects eee 2 5 Declaration of Hazardous Substances DOHS o e eeeecceccesssessssesesseesseessesseesseesseessesseesseesseesees 3 THe instrument and software sessesseeseeseeseeseeseeseerseesessceseeseessessessesseeseeseessesses 3 1 KTA pure instrument Overview voceescccssssssssesssssssssssssssssssssssssssesessssssssssesssssssssssssssssesssssseeeesee 32 UNICORN SOTOWOTE ener OU ne a N EETA R 321 UNICORN SOV Gre Over Vie W ssnin Tetin ERA 3 2 2 System Control module u cescsessssssssesssssssessssesssessssecsssecsssssssessssssssecssuecssessssecssscssuecsssecssessssecesneen A O e avarsrasiacaresnivineivascptacisininenteneieacataeclaenieses ieee Ak enepar O E E E E EEEE A 4 1 1 Belver and TOTOE rrr aetna te ean RE RANET eee en ten ee 4 1 2 R m regulere nle
28. E 8 Reference information 8 3 Check Change the Node ID of a module 8 3 Check Change the Node ID of a module Introduction Node ID is a unit number designation that is used by the instrument to distinguish be tween several units of the same type All standard valves and available optional modules are pre configured to give the desired function However the function of a valve or module can be changed by changing the Node ID Also in a troubleshooting situation it may be useful to check a valve s or module s Node ID Note The function of a valve or module is defined by its Node ID not by its physical position Node ID for core modules The table below lists the Node ID for the core modules Core module Node ID System pump A P9 A P9HA System pump B P9 B P9H B Pressure monitor R9 Mixer M9 Injection valve Node ID for optional modules V9 Inj V9H Inj The table below lists the Node ID for the optional modules Module Node ID Inlet valve A V9 IA V9H IA 0 Inlet valve B V9 IB V9H IB 1 Inlet valve AB V9 IAB V9H IAB 3 Inlet valve X1 V9 IX V9H IX 15 Inlet valve X2 V9 IX V9H IX 16 Mixer valve V9 M V9H M 22 AKTA pure Operating Instructions 29 0229 97 AE 8 Reference information 8 3 Check Change the Node ID of a module Module Sample inlet valve Label V9 IS V9H IS Nole lt H B Loop valve V9 L V9H L Column v
29. H Cs OH valve V9 pH V9H pH AKTA pure Operating Instructions 29 0229 97 AE 55 3 The instrument and software 3 1 AKTA pure instrument overview Module Label in KTA pure 25 KTA pure 150 Outlet valves Versatile valve UV monitors Conductivity monitor External air sensor Fraction collectors I O box Sample pump Core modules Core module Description System pump P9 A or P9H A A high precision pump which delivers buffer or sample in purification runs System pump P9 B or P9H B A high precision pump which delivers buffer in purification runs Pressure monitor R9 Reads the system pressure after System pump A and System pump B 36 KTA pure Operating Instructions 29 0229 97 AE 3 The instrument and software 3 1 AKTA pure instrument overview Core module Description Mixer M9 Mixes the buffers delivered from the system pumps to a homoge neous buffer composition Three Mixer chambers are available for AKTA pure 25 their volumes are 0 6 ml 1 4 ml mounted at delivery and 5 ml Three Mixer chambers are available for AKTA pure 150 Their volumes are 1 4 ml mounted at delivery 5 ml included in delivery and 15 ml CAUTION Risk of explosion Do not use Mixer chamber 15 ml with an KTA pure 25 system configura tion The maximum pressure for Mixer cham ber 15 ml is 5 MPa Injection valve V9 Inj or V9H Inj Directs sample onto t
30. N software 3 2 UNICORN software Introduction This section gives an overview of the UNICORN software It also describes the System Control module To learn more about System Control and the other three modules Administration Method Editor and Evaluation see the UNICORN documentation package In this section This chapter contains the following sections Section See page 3 2 1 UNICORN software overview 45 3 2 2 System Control module 46 44 AKTA pure Operating Instructions 29 0229 97 AE 3 The instrument and software 3 2 UNICORN software 3 2 1 UNICORN software overview 3 2 1 UNICORN software overview Introduction This section gives a brief overview of the UNICORN software a complete package for control supervision and evaluation of chromatography instruments and purification runs From hereon UNICORN refers to compatible versions of the software The examples given in this manual are from UNICORN 6 4 UNICORN modules overview UNICORN consists of four modules Administration Method Editor System Control and Evaluation The main functions of each module are described in the following table Module Main functions Administration Perform user and system setup system log and database administration Method Editor Create and edit methods using one or a combination of e Predefined methods with built in application support e Drag and drop function to build methods with relevant steps e Line by l
31. XXX VAC Serial no XXXXXXX Frequency XX XX Hz Mfg Year 2013 Max Power XXX VA Fuse XX X XXAL 250 V Protection Class XXXX m ll AED CER nee 4001767 MAW AT GE Healthcare Bio Sciences AB Made in Sweden 751 84 Uppsala Sweden 6 Conforms to ANSI UL Std 61010 1 Cert to CAN CSA Std C22 2 No 61010 1 Rating label The rating label is located on the back of the instrument Mains In Mains Out Moinsin 100 240VAC 50 60Hz iL ol Fuse TIDAL250 Power 1100 VA max Mains Out os Moins in 24 mos AKTA pure Operating Instructions 29 0229 97 AE 23 2 Safety instructions 2 2 Labels Connector label The connector label is located on the back of the instrument Computer Jo JYJY e UniNet 9 Note Connectors 1 8 shall be plugged when not in use I O box E9 instrument label The O box serial number is printed on the I O box instrument label located underneath the O box 29011361 O box E9 Code no 28993499 Voltage 32 V UniNet 9 interface Serial no 1111112 Mfg Year 2012 Max Power 2 VA Protection Class P20 J XB CER AAW A GE Healthcare Bio Sciences AB Made in Sweden 751 84 Uppsala Sweden Safety symbols The following safety symbols are used in the labels Meaning Warning Read the Operating Instruction before using the system Do not open any covers or replace parts unless specifically stated in the Operating Instructions 24 KTA pure
32. alve Sample loop or Superloop Column valve Column UV monitor Conductivity monitor Flow restrictor Outlet valve Fraction collector Waste ports The table below shows the waste ports of the Injection valve and outlet valves Valves and ports Illustrations Injection valve V9 Inj or V9 H Inj a D LoopE Waste ports e W1 W2 Col LoopF V9 Inj AKTA pure Operating Instructions 29 0229 97 AE 97 5 Prepare the system for a run 5 2 Prepare the flow path Valves and ports Illustrations Outlet valve V9 Os or V9H Os Waste port eo W Note The waste ports on Out let valve V9 O and V9H O are also labelled W Note If the configuration of the AKTA pure instrument includes a pH valve V9 pH or V9H ph there will be an additional waste port labelled W3 Prepare the waste tubing Make sure that the waste tubing is prepared according to the instructions in Section 4 2 4 Prepare waste tubing on page 71 Prepare the outlet tubing Connect tubing to the outlet ports of the outlet valve that are to be used during the run If no fraction collector is used immerse the outlet tubing in suitable tubes or flasks If a fraction collector is used make sure that tubing is connected between the fraction collector and the Frac port on the outlet valve and prepare the fraction collector for a run Plug unused valve ports It is recommended to p
33. alve 5 columns V9 C V9H C Pre column pressure monitor N A Post column pressure monitor N A Column valve 1 column V9 Cs V9H Cs PH valve V9 pH V9H pH Outlet valve 10 outlets V9 0 V9H O Outlet valve 1 outlet V9 Os V9H Os Versatile valve V9 V V9H V Versatile valve 2 V9 V V9H V Versatile valve 3 V9 V V9H V Versatile valve 4 V9 V V9H V UV monitor fixed U9 L UV monitor 2nd U9 L 1 UV monitor variable U9 M 0 UV detector U9 D 0 Conductivity monitor C9 0 External air sensor L9 0 External air sensor 2 L9 1 External air sensor 3 L9 2 External air sensor 4 L9 5 Fraction collector cassettes F9 C 0 Fraction collector round F9 R 0 Fraction collector round 2nd F9 R 1 Sample pump P9 S P9H S 2 1 O box E9 0 AKTA pure Operating Instructions 29 0229 97 AE 8 Reference information 8 3 Check Change the Node ID of a module Module Noo m 1B O box 2nd Check Change the Node ID Step Action 1 If applicable remove the module according to the instruction in AKTA pure User Manual 2 The Node ID of a module is set by the position of an arrow on a rotating switch at the back of the module Valve modules have two rotating switches as shown in the image below e the first switch labeled A sets the tens and e the second switch labeled B sets the units For example to set the Node ID
34. amethod 6 Runamethod About this chapter This chapter describes the safety aspects of performing a run and how to shut down and clean the system after a run For detailed information about how to run the system see UNICORN System Control Manual In this chapter This chapter contains the following sections Section See page 6 1 Before you start 110 6 2 Applying the sample 113 6 3 Start a method run 116 6 4 Monitor the run 118 6 5 After run procedures 119 KTA pure Operating Instructions 29 0229 97 AE 109 6 Runa method 6 1 Before you start Ol Before you start Introduction 110 Before starting a run it is necessary to read and understand the information in this section and to perform the checks listed below CAUTION Reversed Phase Chromatography RPC runs with 100 acetoni trile in KTA pure Always replace the PEEK tubing between the used system pump and the pump pressure monitor with orange PEEK tubing i d 0 5 mm before running RPC with 100 acetonitrile The tubing to be replaced is green for 25 ml min systems and beige for 150 ml min systems Set the System pressure alarm to 10 MPa for 25 ml min systems CAUTION Always use appropriate personal protective equipment during op eration and maintenance of KTA pure CAUTION Hazardous substances When using hazardous chemical and bio logical agents take all suitable protective measures such as wearing protective glasses and glo
35. and electronic equipment must not be disposed of as un sorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment AKTA pure Operating Instructions 29 0229 97 AE 29 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS 2 5 Declaration of Hazardous Substances DoHS AB HESJ T11364 2006 E FERS MRR ER PERM AASR RABE The following product pollution control information is provided according to SJ T11364 2006 Marking for Control of Pollution caused by Electronic Information Products BSS mR Alpi Explanation of Pollution Control Label Aine WAS SARIS I T11363 2006 E FERA em PABA SMA 205 EZR PRENABASUA MERKATA MHRA RA PF mELREANAE ABASMWAFAREREMAE APEA oo RAN MRE KP BSARNEAR UP GKrPSRSHNRR BUA 7A RUE Pir FR A AY R52 A BBR DR oF PER EET IE me AP RE TT on ESF at EY BES A RF EEK on PARAM ESA Oo REA SIAR eA APRs FH BRUM FARAPR AT ELEN ASHORE RR MER Meee SRA EOTA REAR ASS BB DA PRUE PIT AR BA AEA Gn BY oe 52 A BAB ASF Ga Te EFA A on 25 RNT HE PSE A SO a Bh I SS Sh This symbol indicates the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Products The number in the symbol is the Environment friendly Use Period EFUP which indi
36. cates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating con ditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain the declared EFUP the product shall be operated normally ac cording to the instructions and environmental conditions as defined in the product manual and periodic maintenance schedules specified in Product Maintenance Pro cedures shall be followed strictly Consumables or certain parts may have their own label with an EFUP value less than the product Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures This product must not be disposed of as unsorted municipal waste and must be collected separately and handled properly after decommissioning 30 AKTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS Bs A SWART ANAMRES Name and Concentration of Hazardous Substances mt aSsA SWANTANahReS Table of Hazardous Substances Name and Concentration ab TEE EN Be AS WRI Component name Hazardous substance 0 IK if ROTER ZR Cr6 29 0182 24 29 0182 25
37. cautions WARNING Do not operate AKTA pure in any other way than described in the Operating Instructions WARNING Operation and user maintenance of AKTA pure should be performed by properly trained personnel only WARNING Before connecting a column to AKTA pure read the instructions for use of the column To avoid exposing the column to excessive pressure make sure that the pressure limit is set to the specified maximum pressure of the column AKTA pure Operating Instructions 29 0229 97 AE 13 2 Safety instructions 2 1 Safety precautions WARNING Do not use any accessories not supplied or recommended by GE WARNING Do not use AKTA pure if it is not working properly nor if it has suf fered any damage for example e damage to the power cord or its plug e damage caused by dropping the equipment e damage caused by splashing liquid onto it NOTICE Avoid condensation by letting the instrument equilibrate to ambient temperature Flammable liquids CAUTION Fire Hazard Before the system is turned on make sure that there is no unintentional leakage of flammable liquids or other buffers in AKTA pure or tubing CAUTION Explosion hazard To avoid building up an explosive atmosphere when using flammable liquids make sure that the room ventilation meets the local requirements 14 AKTA pure Operating Instructions 29 0229 97 AE
38. cordance with the settings in the method to be run Before preparing the system check the settings in the Method Editor and make sure that all accessories to be used are available Checklist Make sure the system is prepared in accordance with the settings in the method to be run Depending on configuration remember to check e which valve ports to use for inlets and outlets e which column type to use e which column position to use e which buffers and samples to prepare e which sample application technique to use e that the pH electrode is connected and calibrated CAUTION Reversed Phase Chromatography RPC runs with 100 acetoni trile in AKTA pure Always replace the PEEK tubing between the used system pump and the pump pressure monitor with orange PEEK tubing i d 0 5 mm before running RPC with 100 acetonitrile The tubing to be replaced is green for 25 ml min systems and beige for 150 ml min systems Set the System pressure alarm to 10 MPa for 25 ml min systems 94 AKTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 2 Prepare the flow path 5 2 Prepare the flow path Introduction The flow path is defined by the user and may contain tubing valves pumps and monitors This section gives an overview of a flow path and describes how to prepare the flow path before a run CAUTION Fasten bottles and cassettes Always fasten bottles and cassettes to the rails at the front and si
39. cture The Process picture pane displays the current flow path during the run and can be used to control the run Color indication is applied as shown in the table below Real time data from monitors are also displayed in the process picture See illustration below Indication Open flow path with flow Closed flow path or an open path without flow ystem flow 5 998 m 53 0 prec 0 31 mPa 0 551 ma 37 12 ms em pH 7 83 sample flow 0 000 mi n eee Deitac 0 13 mPa is 5 Manual load By pass u Al E A R P z AS Ew lur oH valve utlet Er Si lt a 14 M mE SyP Anl ujm uv m Cond mm Resistor mmi Frac current m 681 mm e L 5 Dh a 118 KTA pure Operating Instructions 29 0229 97 AE 6 Runa method 6 5 After run procedures 6 5 After run procedures Introduction This section describes how to clean the instrument and columns after a chromatographic run and how to prepare the system for storage The instrument and the columns should be cleaned between the runs This will prevent for example sample contamination protein precipitation and column clogging If the instrument is not going to be used for a couple of days or longer the instrument columns and the pH flow cell should be filled with storage solution For further information about cleaning and maintenance procedures see AKTA pure System Handbook Tip To clean and fill the instrument and columns with storage solution use System CIP and Column CIP eithe
40. d currently connected users are dis played KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 Installation About this chapter This chapter provides the necessary instructions to enable users and service personnel to e unpack KTA pure when delivered from the factory e install the instrument e install the computer e install the software Read the entire Installation chapter before starting to install AKTA pure In this chapter This chapter contains the following sections Section See page 4 1 Site preparation 50 4 2 Hardware installation 60 4 3 Software installation 78 4 4 Start UNICORN and connect to system 19 4 5 Prime inlets and purge pump heads 82 4 6 Performance test 90 4 7 Activate Power save 91 KTA pure Operating Instructions 29 0229 97 AE 49 4 Installation 4 1 Site preparation 4 1 Site preparation Introduction This section describes the site planning and the preparations necessary for the installation of AKTA pure The purpose is to provide planners and technical staff with the data needed to prepare the laboratory for the installation The performance specifications of the system can be met only if the laboratory environ ment fulfills the requirements stated in this chapter The time spent in preparing the laboratory will contribute to the long term performance of the system In this section This section contains the following subsections Section See pag
41. de panel Use appropriate holders for bottles Shattered glass from falling bottles may cause injury Spilled liquid may cause fire hazard and personal injury CAUTION Max weight on Buffer tray Do not place containers with a volume of more than 5 liters each on the Buffer tray The total allowed weight on the Buffer tray is 20 kg CAUTION Avoid spillage and overflow Make sure that the system is prepared according to the settings in the method to be run For example make sure that the waste tubing Is inserted in an appropriate waste container and secured in place CAUTION Avoid spillage and overflow Make sure that the waste tubing is inserted in an appropriate waste container and secured in place gt gt KTA pure Operating Instructions 29 0229 97 AE 95 5 Prepare the system for a run 5 2 Prepare the flow path Illustration of the flow path The illustration below shows the flow path for a typical system configuration The indi vidual instrument modules are presented in the table below The configuration of the system Is defined by the user 5 j W1 W2 Description 1 Pressure monitor 2 Sample pump 3 Sample inlet valve 96 AKTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 2 Prepare the flow path Description Inlet valve System pump B System pump A Pressure monitor Mixer Injection v
42. design with all liquid handling modules placed on the exterior of the instrument Buffer vessels are placed on the Buffer tray on top of the instrument The liquid handling modules and the instrument control panel are located on the front of the instrument It is recommended that the fraction collectors the sample pump and the O box are placed on the left side of the instrument and the computer on the right side of the instrument Instrument configurations AKTA pure is available with two core module configurations one for flow rates up to 25 ml min and one for flow rates up to 150 ml min In this manual they are referred to as AKTA pure 25 25 ml min and AKTA pure 150 150 ml min The table below shows some of the operational limits of AKTA pure 25 and AKTA pure 150 AKTA pure Parameter Flow rate Limits AKTA pure 25 0 001 25 ml min Note When running the Column packing flow instruction the maximum flow rate is 50 ml min AKTA pure 150 0 01 150 ml min Note When running the Column packing flow instruction the maximum flow rate is 300 ml min Max operating pressure AKTA pure Operating Instructions 29 0229 97 AE 20 MPa 5 MPa 55 3 The instrument and software 3 1 AKTA pure instrument overview Illustrations of the main parts of the instrument The illustrations below show the location of the main parts of the instrument Function Wet sides Buffer tray Ho
43. displays a constant light gt m KTA pure Operating Instructions 29 0229 97 AE 41 3 The instrument and software 3 1 AKTA pure instrument overview 42 Display State Description Both the Power Communica Run A run is ongoing tion indicator and Continue button display a constant light gt The Power Communication in Wash A wash instruction or a pump dicator displays a constant light synchronization is ongoing and the Continue button flashes slowly Hold A run has been put on hold Both the Power Communica Pause A run has been paused tion indicator and Pause button display a constant light AKTA pure Operating Instructions 29 0229 97 AE Display The Power Communication in dicator displays a constant light and the Alarm and error indica tor flashes i gt m Alarms and errors 3 The instrument and software 3 1 KTA pure instrument overview Description The system has been paused due to an alarm To resume the run acknowledge the alarm and continue the run in UNICORN The Power Communication in dicator displays a pulsating light Power save The system is in power saving mode All indicators are lit in a wave pattern KTA pure Operating Instructions 29 0229 97 AE Re program ming A module is being re pro grammed to be compatible with the current instrument configu ration 43 3 The instrument and software 3 2 UNICOR
44. e 4 1 1 Delivery and storage oul 4 1 2 Room requirements 53 4 1 3 Site environment 56 4 1 4 Power requirements 58 50 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 1 Site preparation 4 1 1 Delivery and storage 4 1 1 Delivery and storage Introduction This section describes the requirements for receiving the delivery box and storing the instrument before installation CAUTION Heavy object Use proper lifting equipment or use two or more persons when moving the instrument All lifting and moving must be performed in accordance with local regulations When you receive the delivery e Record on the receiving documents if there is any apparent damage on the delivery box Inform your GE representative of such damage e Move the delivery box to a protected location indoors AKTA pure delivery box The AKTA pure instrument is shipped in a delivery box with the following dimensions and weight Contents Dimensions mm KTA pure instru w660 x h850 x d600 up to 64 kg ment with acces sories KTA pure Operating Instructions 29 0229 97 AE 51 4 Installation 4 1 Site preparation 4 1 1 Delivery and storage Storage requirements The delivery box should be stored in a protected place indoors The following storage requirements must be fulfilled for the unopened box Parameter Ambient temperature storage Allowed range 25 C to 60 C Relative humidity Equipment
45. e avoided in order to not damage the pressure sensors If you are cleaning separation media using 1 M hydrochloric acid use loop injections of the acid and make sure that the column is not mounted on the column valve V9 C The column valve V9 C contains a pressure sensor which can be damaged by 1 M hydrochloric acid Long time use of 0 2 M HCI connected to the Quaternary valve as part of a BufferPro recipe is acceptable The solution becomes diluted further down in the system If sodium hypochlorite is used as sanitizing agent instead of 2 M sodium hydroxide use a concentration up to 10 Organic solvents Reversed phase chromatography of proteins works well with 100 acetonitrile and ad ditives trifluoroacetic acid TFA up to 0 2 or formic acid up to 5 Strong organic solvents like ethyl acetate 100 acetone or chlorinated organic solvents should be avoided These might cause swelling of plastic material and reduce the pressure tolerance of PEEK tubing For this reason flash chromatography and straight normal phase chromatography is generally not recommended on the system AKTA pure Operating Instructions 29 0229 97 AE 8 Reference information 8 2 Chemical resistance guide Assumptions made The ratings are based on the following assumptions e Synergy effects of chemical mixtures have not been taken into account e Room temperature and limited overpressure is assumed Note Chemical influences are time and pressure de
46. ed with care to avoid injuries Warnings WARNING Protective ground The AKTA pure instrument must always be connected to a grounded power outlet WARNING Only use grounded power cords delivered or approved by GE WARNING Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect Cautions gt CAUTION Heavy object Use proper lifting equipment or use two or more persons when moving the instrument All lifting and moving must be performed in accordance with local regulations KTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 1 Safety precautions gt CAUTION Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before con necting the power cord Notices NOTICE Vents on AKTA pure To ensure adequate ventilation keep papers and other objects away from the vents of AKTA pure NOTICE Disconnect power To prevent equipment damage always switch off power to the AKTA pure instrument before an instrument module is removed or installed or a cable is connected or discon nected NOTICE Misuse of UniNet 9 connectors The UniNet 9 connectors at the rear panel should not be confused with Firewire connectors Only connect GE marked products to the UniN
47. ements The table below specifies the power requirements Parameter Requirement Supply voltage 100 240 V 10 Frequency 50 60 Hz Transient level Overvoltage category Il 58 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 1 Site preparation 4 1 4 Power requirements Parameter Requirement Typical power consump 300 VA in state Run Hen 165 VA in state Ready 25 VA in state Power save Maximum power con 1100 VA sumption Number of sockets 1 socket per instrument up to 3 sockets for computer equipment Type of sockets EU or US plugs Grounded mains sockets fused or protect ed by equivalent circuit breaker Location of sockets Maximum 2 m from the instrument due to length of mains cable Extension cables can be used if required Quality of power The mains power supply must be stable and conform to specifications at all times to ensure reliable operation of AKTA pure There should be no transient or slow changes in average voltage outside the limits specified above AKTA pure Operating Instructions 29 0229 97 AE 59 4 Installation 4 2 Hardware installation 42 Hardware installation Introduction This section describes the installation procedure for AKTA pure WARNING Protective ground The AKTA pure instrument must always be connected to a grounded power outlet WARNING Only use grounded power cords delivered or approved by GE
48. escription KTA pure Operating Instructions DVD packages with Instrument Configuration software and manuals 66 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 2 Install the computer equipment 4 2 2 Install the computer equipment Introduction The computer is either e Supplied as a part of the KTA pure delivery e Supplied locally Unpacking and installing Unpack and install the computer according to the manufacturer s instructions NOTICE Any computer used with the equipment shall comply with IEC 60950 and be installed and used according to the manufacturer s instruc tions KTA pure Operating Instructions 29 0229 97 AE 67 4 Installation 4 2 Hardware installation 4 2 3 Connect system units 4 2 3 Connect system units Introduction The following interconnections must be made e Power supply to the KTA pure instrument e Power supply to the computer equipment e Network connection between the computer and the KTA pure instrument WARNING Only use grounded power cords delivered or approved by GE CAUTION Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before con necting the power cord 68 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 3 Connect system units Connector illustration The i
49. et 9 connectors Do not disconnect or move the UniNet 9 bus cable oO o NOTICE Any computer used with the equipment shall comply with IEC 60950 and be installed and used according to the manufacturer s instruc tions AKTA pure Operating Instructions 29 0229 97 AE 17 2 Safety instructions 2 1 Safety precautions System operation 18 Warnings WARNING Electrical shock hazard after spillage If there is a risk that large volumes of spilled liquid may penetrate the casing of AKTA pure immediately switch off AKTA pure disconnect the power cord and contact an authorized service engineer Cautions CAUTION Fasten bottles and cassettes Always fasten bottles and cassettes to the rails at the front and side panel Use appropriate holders for bottles Shattered glass from falling bottles may cause injury Spilled liquid may cause fire hazard and personal injury CAUTION Hazardous chemicals during run When using hazardous chemi cals run System CIP and Column CIP to flush the entire system tubing with distilled water before service and maintenance gt gt CAUTION pH electrode Handle the pH electrode with care The glass tip may break and cause injury KTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 1 Safety precautions CAUTION Max weight on Buffer tray Do not place containers with a volume of more t
50. g Instructions 29 0229 97 AE 91 5 Prepare the system for a run 5 Prepare the system for a run About this chapter This chapter describes the preparations necessary to prepare the system before starting a run WARNING Do not use KTA pure if it is not working properly nor if it has suf fered any damage for example e damage to the power cord or its plug e damage caused by dropping the equipment e damage caused by splashing liquid onto it WARNING Always use appropriate personal protective equipment during op eration and maintenance of AKTA pure system WARNING Do not use any accessories not supplied or recommended by GE CAUTION Fire Hazard Before the system is turned on make sure that there is no unintentional leakage of flammable liquids or other buffers in AKTA pure or tubing 92 AKTA pure Operating Instructions 29 0229 97 AE In this chapter This chapter contains the following sections 5 Prepare the system for a run Section See page 5 1 Before you prepare the system 94 5 2 Prepare the flow path 95 5 3 Prime inlets and purge pump heads 99 5 4 Connect a column 100 5 5 Pressure alarms 105 5 6 Prepare for a run at cold room temperature 108 93 AKTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 1 Before you prepare the system 5 1 Before you prepare the system Introduction It is important to prepare the system in ac
51. han 5 liters each on the Buffer tray The total allowed weight on the Buffer tray is 20 kg CAUTION Disconnect power Always switch off power to AKTA pure before cleaning any of its components unless stated otherwise in the user documentation CAUTION Avoid spillage and overflow Make sure that the system is prepared according to the settings in the method to be run For example make sure that the waste tubing Is inserted in an appropriate waste container and secured in place CAUTION Avoid spillage and overflow Make sure that the waste tubing Is inserted in an appropriate waste container and secured in place CAUTION Risk of explosion Do not use Mixer chamber 15 ml in the low flow system The maximum pressure for Mixer chamber 15 ml is 5 MPa CAUTION Fasten the waste tubing During operation at high pressure the AKTA pure instrument may release bursts of liquid in the waste tubing Securely fasten all waste tubing to the AKTA pure instrument and to the waste vessel gt PP D KTA pure Operating Instructions 29 0229 97 AE 19 2 Safety instructions 2 1 Safety precautions Notices NOTICE Keep UV flow cell clean Do not allow solutions containing dissolved salts proteins or other solid solutes to dry out in the flow cell Do not allow particles to enter the flow cell as damage to the flow cell may occur NOTICE Avoid condensation If AKTA pure is kept i
52. he system pumps The total buffer volume to be used for emptying and washing the sample loop is set in the Method Editor in the Phase Properties tab of the Sample Application phase at Empty loop with Phase Properties Sample Applicaton Use the same flow rate as in Method Settings Flow rate 10 000 ml min 0 000 25 000 Inject sample from loop Inject sample directly onto column Fill the loop using Manual load ey Enon ie Capillary loop v Prime sample inlet with 6 00 mi Sample inlet Sample volume 0 ml Use the same inlets as in Method Settings InetA AN InletB BI a oo C Fill the system with the selected buffer Tip Empty the sample loop with a buffer volume that exceeds the volume of the loop This will ensure that the loop is completely emptied AKTA pure Operating Instructions 29 0229 97 AE 115 6 Runa method 6 3 Start a method run 6 3 Start a method run Introduction This section describes how to start a run using a previously created method For further information on method creation please refer to UNICORN Method Manual Choose and start a method The instruction below describes how to open a method and start a run Step Action 1 Open the System Control module and click the Open Method Navigator button z Result The Method Navigator pane opens Method Navigator Open wa Fe Methods Method Gu Folder name
53. he column Optional modules Module Description Inlet valve Inlet valve V9 IA or V9H IA Inlet valve with seven inlets Inlet valve V9 IB or V9H IB Inlet valve with seven inlets Inlet valve V9 IAB Inlet valve with two A inlets and two B in or V9H IAB lets Inlet valve V9 IX or V9H IX Inlet valve with eight inlets Sample inlet valve V9 IS or Inlet valve with seven inlets These valves V9H IS require the external module Sample pump 32 OF 59h Mixer valve Mixer valve V9 M Directs the flow to the Injection valve via or VQH M the mixer or by bypassing the mixer Loop valve Loop valve V9 L Enables the use of up to five loops connect or VOH L ed to the instrument AKTA pure Operating Instructions 29 0229 97 AE 537 3 The instrument and software 3 1 AKTA pure instrument overview Column valve Module Column valve V9 C or V9H C Description Connects up to five columns to the instru ment and directs the flow onto one col umn ata time Column valve V9 Cs or V9H Cs Connects one column to the instrument PH valve OH valve V9 pH or V9H pH Enables in line monitoring of pH during the run Outlet valve Outlet valve V9 O or V9H O Directs the flow to the fraction collector to any of the ten outlet ports or to waste Outlet valve V9 Os or V9H Os Directs the flow to the fraction collector to the outlet port or to waste Versatile valve Ver
54. hical conventions 6 U UNICORN 45 connect to system 81 Log on 79 Start 79 System Control module 46 Unpack the instrument 61 W Waste ports 97 Waste tubing 71 prepare 71 98 Weight instrument 55 139 Page intentionally left blank Page intentionally left blank For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA GE and GE monogram are trademarks of General Electric Company AKTA and UNICORN are trademarks of General Electric Company or one of its subsidiaries Decon is a trademark of Decon Laboratories Ltd TRITON is a trademark of Union Carbide Chemicals and Plastic Company Inc Tween is a trademark of Croda Group of Companies Microsoft and Windows are registered trademarks of Microsoft Corporation All other third party trademarks are the property of their respective owners Any use of UNICORN is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreement is available on request 2012 2015 General Electric Company All rights reserved First published Sep 2012 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact
55. ine text editing The interface provides easy viewing and editing of run properties System Control Start monitor and control runs Real time flow scheme shows current flow path valve positions and monitor values Evaluation Open results evaluate runs and create reports e The default Evaluation module includes a user inter face optimized for workflows like quick evaluation compare results and work with peaks and fractions To perform operations like Design of Experiments users can easily switch to Evaluation Classic KTA pure Operating Instructions 29 0229 97 AE 45 3 The instrument and software 3 2 UNICORN software 3 2 2 System Control module 5 2 2 Introduction System Control module The System Control module is used to start view and control a manual or method run System Control panes 46 As seen in the following illustration three panes are shown in the System Control module by default The Run Data pane 1 presents current data in numerical values The Chromatogram pane 2 illustrates data as curves during the entire run The current flow path is illustrated in the Process Picture 3 which allows manual inter actions with the system and provides feedback on run parameters File Edt View Manual System Tools Help T ly E amp __ LEE 206 Test id 40 Resour E Run Data xX _ System state Acc voume System fow System pressure PreC Method Run 76 7 mi 5 99
56. is active 4 In the Process Picture e Click on the buffer inlets e Select the position of one of the inlets that will be used at the beginning of the run Buffer inlets Pumps A w A l PjJ 8606 Inlet valve AB a Result The inlet valve switches to the selected port AKTA pure Operating Instructions 29 0229 97 AE 85 4 Installation 4 5 Prime inlets and purge pump heads Step Action 5 86 In the Process Picture e Click on the Pumps e Set the System flow to 1 0 ml min for KTA pure 25 or 10 0 ml min for AKTA pure 150 e Click Set flow rate 1 000 mimin Set flow rate el 100 8 aSa Result A system flow starts Connect a 25 to 30 ml syringe to the purge valve of the left pump head of System pump B Make sure that the syringe fits tightly into the purge con nector Open the purge valve by turning it counter clockwise about three quarters of a turn Draw a small volume of liquid slowly into the syringe with a rate of about 1 ml per second Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 5 Prime inlets and purge pump heads Step Action 9 Connect the syringe to the purge valve on the right pump head of System pump B and repeat steps 6 to 8 Keep the system flow running Validate purge of pump B Follow the instructions below to check that there
57. is no air left in the pump after performing a purge Step Action 1 In the Process Picture e Click on the Injection valve and select Manual load Result The Injection valve switches to manual load position Manwal load Manual load Y syp AKTA pure Operating Instructions 29 0229 97 AE 87 4 Installation 4 5 Prime inlets and purge pump heads Step Action 2 Make sure that the pump flow is on 5 In the Chromatogram pane Check the PreC pressure curve If the PreC pressure do not stabilize within a few minutes there may be air left in the pump Refer to AKTA pure User Manual for a troubleshooting guide Purge System pump A Purge both pump heads of System pump A by following the same procedure as in Purge System pump B on page 84 but replace step 3 with the following actions In the Process Picture e Click on the pumps e Set Conc B to 0 B J umps j 0 000 ml min Set flow rate A Conc B J _00 8 Set Bu 100 B Pump wash A B Al wv Start pump A wash B1 wv Start pump B wash e Click Set B Result Only System pump A is active Validate purge of pump A Follow the procedure described in Validate purge of pump B on page 87 to check if there is air left in the pump 88 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 5 Prime inlets and purge pump heads End the run Click the End icon in the System Control toolbar to end the run I
58. jection valve to column r System Settings Akta Pure Instructions Pressure alarms Air sensor O box 0 50 ES waco collection Length 0 2000 160 mm Parameters for Tubing Injection valve to column I D Tubing Sanden pump x Tiedon vil Delay volume pH valve Delay volume Monitor to outlet val Delay volume Monitor to frac Delay volume Monitor to frac 2 wa r Set Parameters To Strategy Default Values Cancel 3 e Select the inner diameter of the tubing between the injection valve and the column from the I D drop down list e Type in the tubing Length 4 If the sample pump is used e Select Tubing Sample pump to injection e Set tubing i d and length see step 3 s Click OK Note The system now calculates the Pre column pressure Pre column pressure alarms It is important that the pre column pressure alarm is set during all runs where a column is used The pressure alarm can be set in e the method to be run e the System Settings dialog or e during a manual run Pre column pressure alarm limits are automatically set in the method when a column from the column list is selected in the method Refer to UNICORN Method Manual for more information on pressure alarms 106 AKTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 5 Pressure alarms For some columns the max delta column pressure media is significantly lower
59. lder rails Instrument control panel Power switch Ventilation panel 34 AKTA pure Operating Instructions 29 0229 97 AE 3 The instrument and software 3 1 AKTA pure instrument overview Available modules The modular design allows the user to customize AKTA pure in multiple ways The system is always delivered with the core modules of the selected configuration but one or more optional modules may be added to the flow path The table below contains information on the core modules and the optional modules of AKTA pure 25 and AKTA pure 150 The sections that follows contain descriptions of the modules Note The valves for AKTA pure 25 and AKTA pure 150 are compatible with both systems but for the best performance the specific valve type should be used The narrow channels in the valves for AKTA pure 25 will give too high back pressure if used above 50 ml min The larger volumes in the H valves for AKTA pure 150 may decrease resolution and increase peak broadening if used in AKTA pure 25 Module Label in AKTA pure 25 AKTA pure 150 System pump A P9 A P9H A System pump B P9 B P9H B Pressure R9 R9 monitor Mixer M9 M9 Injection valve V9 Inj V9H Inj Inlet valve A V9 IA V9H IA Inlet valve B V9 IB V9H IB Inlet valve AB V9 IAB V9H IAB Inlet valve IX V9 IX V9H IX Sample inlet valve V9 IS V9H IS Mixer valve V9 M V9H M Loop valve V9 L V9H L Column valves V9 C V9H C V9 Cs V9
60. llustration below shows where the connectors are located on the AKTA pure instru ment For connectors on the computer equipment refer to the manufacturer s documen tation Mains In Mains Out moinsin 100 240vac 50 60Hz fuse sTIOAL250V ll willis Ute TAT BN nie Connect power to the AKTA pure instrument Follow the instruction below to connect power to the AKTA pure instrument Step Action 1 Select the correct power cord to be used Each instrument is delivered with 2 alternative power cords e Power cord with US plug 2 m e Power cord with EU plug 2 m Discard the power cord that is not to be used 2 Connect the power cord to the Power input connector on the back of the instrument and to a grounded wall outlet 100 240 V 50 60 Hz KTA pure Operating Instructions 29 0229 97 AE 69 4 Installation 4 2 Hardware installation 4 2 3 Connect system units Connect power to computer equipment Follow the manufacturer s instructions to connect power to the e computer e monitor e local printer if used Connect to network Follow the instructions below to make network connections Step Action 1 Connect a network cable between the network connector Ethernet on the back of the instrument and the computer network card The illustration below shows the symbol of the Ethernet connector 2 If the computer is to be connected to an external network connect a network cable between the main
61. lug all unused valve ports with stop plugs before starting a run See AKTA pure User Manual for information about connectors 98 AKTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 3 Prime inlets and purge pump heads 5 5 Prime inlets and purge pump heads Introduction Before usage of the system pumps It is important to e Prime the inlets fill the buffer inlets with liquid e Purge the system pumps remove air from the pump heads Note Note that the procedures described in this section may have to be adapted if your system configuration differs from the one described in this manual For instructions on how to prime the inlets and purge the pump heads see Section 4 5 Prime inlets and purge pump heads on page 82 AKTA pure Operating Instructions 29 0229 97 AE 99 5 Prepare the system for a run 5 4 Connect a column 54 Connect a column Introduction This section describes how to connect a column to the instrument using a column holder and without introducing air into the flow path Several types of column holders are available for KTA pure WARNING To avoid exposing the column to excessive pressure make sure that the pressure limit is set to the specified maximum pressure of the column Before connecting a column to the KTA pure instru ment read the instructions for use of the column Methods automatically include a pressure alarm based on the specifications of the
62. m external computer Control system UNICORN 6 4 or other compatible version Connection between PC and instrument Ethernet Dimensions W x D x H 535 x 470 x 630 mm Weight excluding computer Power supply up to 53 kg 100 240 V 50 60 Hz Power consumption 300 VA typical 25 VA power save Enclosure protective class IP 21 KTA pure Operating Instructions 29 0229 97 AE 127 8 Reference information 8 1 System specifications Parameter Tubing and connectors AKTA pure 25 e Inlet FEP tubing i d 1 6 mm Tubing connector 5 16 Ferrule yellow 1 8 Pump to Injection valve PEEK tubing i d 0 75 mm Fingertight connector 1 16 After Injection valve PEEK tubing i d 0 50 mm Fingertight connector 1 16 Outlet and waste ETFE tubing i d 1 0 mm Fingertight connector 1 16 Optional tubing kits i d 0 25 mm i d 0 75 mm i d 1 0 mm AKTA pure 150 e Inlet FEP tubing i d 2 9 mm Tubing connector 5 16 Ferrule blue 3 16 Pump to injection valve PEEK tubing i d 1 0 mm 10 32 UNF connections After Injection valve PEEK tubing i d 0 75 mm 10 32 UNF connections Outlet FEP i d 1 6 mm 5 16 24 UNF connections Waste ETFE tubing i d 1 0 mm Fin gertight connector 1 16 Optional tubing kits i d 0 5 mm i d 1 0 mm Environmental ranges Parameter Storage and transport temperature range 25 C to 60 C
63. ml of 20 ethanol 76 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 6 Start the instrument and the computer 4 2 6 Start the instrument and the computer Introduction This section describes how to start the instrument and the computer Instruction Follow the instructions below to start the instrument and the computer Step Action 1 Switch on the instrument by pressing the power switch to the I position A Result The instrument starts and the Instrument control panel displays a white slowly flashing light 2 Turn on the computer and monitor according to the manufacturer s instruc tions AKTA pure Operating Instructions 29 0229 97 AE 77 4 Installation 4 3 Software installation 4 4 Software installation Introduction This section gives an overview of the different UNICORN installation types Detailed information about software installation and configuration is available in the Administration and Technical Manual Software installations UNICORN can be installed as listed below a complete UNICORN installation on a stand alone workstation Full installation a UNICORN database and license server Custom installation and UNICORN software client and instrument server software on a network client station Custom installation It is also possible to define a system as part of the installation configure E licenses configure Windows setting
64. n 1 Prime all inlet tubing to be used during the run 2 Purge System pump B 3 Validate purge of System pump B 4 Purge System pump A 5 Validate purge of System pump A 6 End the run Tip The procedures for purging the pump heads and priming the inlets using the Process Picture are described below It is also possible to perform the proce dures from the Manual instructions dialog Prime inlet tubing Follow the instructions below to fill all A and B inlet tubing to be used in the run with appropriate buffer solution Step Action 1 Make sure that all inlet tubing that is to be used during the method run is placed in the correct buffer 2 Open the System Control module 82 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 5 Prime inlets and purge pump heads Step Action 3 In the Process Picture e Click on the buffer inlets e Select the position of the inlet to be filled Select the positions in reverse alphabetical order and start with the highest number For example if all the four inlets in Inlet valve AB are to be filled fill them in the following order B2 B1 A2 A1 Buffer inlets Pumps aij az 81 52 Result The inlet valve switches to the selected port 4 Connect a 25 to 30 ml syringe to the purge valve of one of the pump heads of the pump thot is being prepared Make sure that the syringe fits tightly into the purge connector 5 Open the purge valve by turning it co
65. n a cold room cold cabinet or similar keep AKTA pure switched on in order to avoid condensation NOTICE Avoid overheating If KTA pure is kept in a cold cabinet and the cold cabinet is switched off make sure to switch off AKTA pure and keep the cold cabinet open to avoid overheating NOTICE Place the computer in room temperature If the KTA pure instru ment is placed in a cold room use a cold room compatible comput er or place the computer outside the cold room and use the Ether net cable delivered with the instrument to connect to the computer Maintenance 20 Warnings WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation AKTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 1 Safety precautions Cautions CAUTION Disconnect power Always switch off power to the AKTA pure in strument before replacing any of its components unless stated otherwise in the user documentation CAUTION Hazardous chemicals and biological agents Before maintenance service and decommissioning wash the AKTA pure instrument with a neutral solution to make sure that any hazardous solvents and biological agents have been flushed out from the system CAUTION Hazardous UV light Always switch off the power to the instrumen
66. nect a column Step Action 5 In the Process Picture e Click on the Column e Select Column down flow Column down flow gt 0 00 MPa Column Result The Column valve switches to position 1 6 In the Process Picture e Click on the Pumps e Enter a low System flow e g 0 2 ml min e Click Set flow rate System flow amp Bi 0 200 mimin 0 25 Conc B a M 0 0 B ma 0 100 A a n Result A system flow of 0 2 ml min starts 102 KTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 4 Connect a column Step Action i When buffer leaves the tubing in a continuous mode and the top part of the column is filled with buffer connect the tubing to the top of the column 8 Connect a piece of tubing to the bottom of the column KTA pure Operating Instructions 29 0229 97 AE 103 5 Prepare the system for a run 5 4 Connect a column Step Action 9 When buffer leaves the tubing at the bottom of the column in a continuous mode connect this piece of tubing to the Column valve Use the port opposite to the one already connected to the column in this example port 1B If no column valve is used connect the tubing to the next module in the flow path 10 Click the End icon in the System Control toolbar to end the run 1 oy 104 AKTA pure Operating Instructions 29 0229 97 AE 5 Prepare the system for a run 5 5 Pressure alarms
67. ons 29 0229 97 AE 53 4 Installation 4 1 Site preparation 4 1 2 Room requirements Space requirements The illustration below shows the space recommended for AKTA pure with Fraction col lector F9 R The illustration below shows the space recommended for AKTA pure with Fraction col lector F9 C Laboratory bench The bench must be clean flat and stable and able to support the weight of AKTA pure see table below 54 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 1 Site preparation 4 1 2 Room requirements Equipment weight AKTA pure instrument up to 53 kg Computer approximately 9 kg Monitor approximately 3 kg AKTA pure dimensions 630 mm 535mm AKTA pure Operating Instructions 29 0229 97 AE 55 4 Installation 4 1 Site preparation 4 1 3 Site environment 4 1 3 Site environment Introduction This section describes the environmental requirements for installation of AKTA pure Operating conditions The following requirements must be fulfilled e The instrument is intended for indoor use only e The room must have exhaust ventilation e The instrument should not be exposed to direct sunlight e Dustin the atmosphere should be kept to a minimum Allowed operating conditions are specified in the table below Parameter Allowed range Ambient temperature operating 4 C to 35 C Ambient temperature storage 25 C to 60 C Relative humidity ope
68. pendent Unless otherwise stated all concentrations are 100 List of chemicals Note A user can be exposed to large volumes of chemical substances over a long time period Material Safety Data Sheets MSDS provide the user with informa tion regarding characteristics human and environmental risks and preventive measures Make sure that you have the MSDS available from your chemical distributor and or databases on the internet Aqueous buffers Continuous use Chemical Concentra CAS no EC no tion Aqueous buffers N A N A pH 2 12 Strong chemicals and salts for CIP Up to 2 h contact time at room temperature Chemical Concentra CAS no EC no tion Acetic acid 75 05 8 200 835 2 Decon 90 N A Ethanol 75 08 1 200 837 3 Methanol 100 67 56 1 200 659 6 Hydrochloric acid 1 0 1M 7647 01 0 231 595 7 lsopropanol 100 67 63 0 200 661 7 Sodium hydroxide 2M 1310 73 2 215 185 5 Sodium hydroxide ethanol 1 M 40 N A AKTA pure Operating Instructions 29 0229 97 AE 131 8 Reference information 8 2 Chemical resistance guide 132 Chemical Concentra CAS no EC no tion Sodium chloride 4M 7647 14 5 231 598 3 Sodium hypochlorite 10 7681 52 9 231 668 3 If hydrochloric acid HCl is used as a cleaning agent when columns are connected to the system the HCI concentration should not exceed 0 1 M in the pressure sensors Remember that the AKTA pure system has pressure sensors in the column valve
69. r 5 System pump A 6 Mixer 7 Outlet valve KTA pure Operating Instructions 29 0229 97 AE 39 3 The instrument and software 3 1 AKTA pure instrument overview Function Injection valve Conductivity monitor Column valve UV monitor illustration of Instrument control panel The Instrument control panel is located to the right on the front of the instrument It shows the current status of the system using four LED lights The Pause and Continue buttons can be used to control an ongoing method run Function Power Communication indicator white Continue button with a green light indicator Pause button with an orange light indicator Alarm and error indicator red 40 KTA pure Operating Instructions 29 0229 97 AE 3 The instrument and software 3 1 AKTA pure instrument overview Status indications The light indicators on the Instrument control panel indicate the current status of AKTA pure The table below describes the different states that can be displayed Display State Description All light indicators are off The instrument is turned off i gt m The Power Communication in Power on The instrument has no commu dicator flashes slowly nication with the Instrument server The Power Communication in Connecting The system is starting up dicator flashes quickly The Power Communication in Ready The instrument is ready to use dicator
70. r as separate predefined methods or as phases in cluded in a chromatographic method CAUTION Hazardous chemicals and biological agents Before maintenance service and decommissioning wash the AKTA pure instrument with a neutral solution to make sure that any hazardous solvents and biological agents have been flushed out from the system System cleaning After a method run is completed perform the following e Rinse the instrument with one or several cleaning solution s e g NAOH buffer solu tion or distilled water using System CIP Note If Column valve V9 C or V9H C is mounted the integrated pressure sensor of the valve allows the system to monitor the post column pressure The limit for the pressure sensor in Column valve V9 C or V9H C is automati cally set so that the UV monitor and the pH monitor are protected from high pressure If Column valve V9 C or V9H C is not mounted make sure to keep the pressure in the system after the column below the pressure limits for the modules in the flow path e lf applicable empty the fraction collector e Clean all spills on the instrument and on the bench using a moist tissue e Empty the waste vessel e Clean the manual injection port of the injection valve AKTA pure Operating Instructions 29 0229 97 AE 119 6 Runa method 6 5 After run procedures e lf applicable clean the pH electrode manually and make sure to leave it in an appro priate buffer See AKTA pu
71. rating 20 to 95 non condensing Altitude Maximum 2000 m Pollution degree 2 Note Do not use buffers with a freezing point close to or below the temperature in the room or in the cold room cabinet Heat output The heat output data is listed in the table below Component Heat output AKTA pure instrument Typically 300 W Maximum 600 W Computer incl monitor and printer Typically 300 W Refer to manufacturer s instructions for more information 56 AKTA pure Operating Instructions 29 0229 97 AE Component Total AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 1 Site preparation 4 1 5 Site environment Heat output Typically 600 W Maximum 900 W 57 4 Installation 4 1 Site preparation 4 1 4 Power requirements 4 1 4 Power requirements Introduction This section describes the power supply requirements for AKTA pure WARNING Protective ground The AKTA pure instrument must always be connected to a grounded power outlet WARNING Only use grounded power cords delivered or approved by GE WARNING Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect CAUTION Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before con necting the power cord Requir
72. re User Manual for detailed instructions System storage If the instrument is not going to be used for a couple of days or longer also perform the following e Fill the system and inlets with storage solution e g 20 ethanol using System CIP Note If Column valve V9 C or V9H C is mounted the integrated pressure sensor of the valve allows the system to monitor the post column pressure The limit for the pressure sensor in Column valve V9 C or V9H C is automati cally set so that the UV monitor and the pH monitor are protected from high pressure If Column valve V9 C or V9H C is not mounted make sure to keep the pressure in the system after the column below the pressure limits for the modules in the flow path e f applicable prepare the pH electrode for storage as described in KTA pure User Manual Column cleaning After a method run is completed perform the following e Clean the column with one or several cleaning solution s using Column CIP Column storage If the column is not going to be used for a couple of days or longer also perform the following e Fill the column with storage solution e g 20 ethanol using Column CIP pH electrode storage If pH monitoring will not be used for a week or longer perform one of the following ac tions e inject new storage solution into the pH flow cell e Replace the pH electrode with the dummy electrode that is installed in the pH valve on delivery
73. s necessary for the UNICORN process pictures in a network deployment configure firewall settings when necessary upgrade UNICORN remove UNICORN installations and set up a system printer 78 AKTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 4 Start UNICORN and connect to system 44 Start UNICORN and connect to system Introduction This section describes how to start and log on to UNICORN and how to connect the in strument to UNICORN Start UNICORN and log on Follow the instructions to start UNICORN and log on to the program A valid e license must be available for the workstation See Administration and Technical Manual for more information about e licenses Step Action 1 Double click the UNICORN icon on the desktop Result The Log On dialog opens Note If there is no connection to the database it is still possible to log on to UNICORN and control a running system The Log On dialog will give the option to start System Control without a database Click Start System Control to proceed to the next Log On dialog AKTA pure Operating Instructions 29 0229 97 AE 79 4 Installation 4 4 Start UNICORN and connect to system 80 Action In the Log On dialog select User Name and enter Password Note It is also possible to select the Use Windows Authentication checkbox and enter a network ID in the User Name field click OK Use Windows Authentication User
74. satile valve V9 V or V9H V A 4 port 4 position valve which can be used to customize the flow path UV monitor UV monitor U9 L Measures the UV absorbance at the fixed wavelength 280 nm UV monitor U9 M Measures the UV Vis absorbance at three wavelengths in the range of 190 700 nm Conductivity monitor Conductivity monitor C9 Measures the conductivity of buffers and eluted proteins Air sensor External air sensor L9 Prevents air from being introduced into the flow path Fraction collector O box Fraction collector F9 C Flexible fraction collector with up to 576 fractions Fraction collector F9 R O box E9 Round fraction collector with up to 350 fractions Receives analog or digital signals from or transfers analog or digital signals to exter nal equipment which has been incorporat ed in the system Sample pump 38 Sample pump S9 or S9H A high precision pump which delivers buffer or sample in purification runs AKTA pure Operating Instructions 29 0229 97 AE Example of a typical configuration of the wet side The descriptions of AKTA pure and the work flow in this manual are based on an AKTA pure that consists of the modules and parts shown in the illustration below 3 The instrument and software 3 1 AKTA pure instrument overview Function 1 Inlet valve 2 Pump rinsing liquid tube 5 System pump B 4 Pressure monito
75. shown in a panel below the process picture modules are called components in the process picture Process Picture l System flow 0 000 mi min 0 0 xs Prec 0 00 wpa 0 000 mau 10 23 m Loop valve Sample flow 0 000 mi min p Deltac 0 00 mpa Inlets Pumps Manual load By pass A ae A Y E w1 Column Ni r NY a P ia M ae F N By pass pm UV Cond f 61 j PF 3 A Available components without a fixed place rs S 2 inlet valve X1 Inletvalve X2 Versatile valve Versatile valve 2 0 000 mau 1 1 1 3 1 3 Air 1 Air 2 UV 2 O box AKTA pure Operating Instructions 29 0229 97 AE 47 3 The instrument and software 3 2 UNICORN software 3 2 2 System Control module System Control toolbar buttons The following table shows the System Control toolbar buttons that are referred to in this 48 manual Button Function Open Method Navigator Opens the Method Naviga tor where available meth ods are listed Button Function Run Starts a method run Hold Suspends the method run while current flow rate and valve positions are sustained Pause Suspends the method run and stops all pumps Continue Resumes for ex ample a held or paused method run End Permanently ends the method run Customize Opens the Cus tomize dialog where curve settings run data groups and run log contents can be set Connect to Systems Opens the Connect to Systems di alog where systems can be connected an
76. t before replacing the UV cell CAUTION Always use appropriate personal protective equipment when de commissioning the equipment CAUTION Cleaning the AKTA pure instrument before decommissioning gt PP D e Wipe the KTA pure instrument and any modules with a damp tissue using a cleaning agent so that no hazardous solvents or biological agents remain on the surface e Perform a system CIP using a neutral solution Make sure that any hazardous solvents or biological agents are flushed out from the system KTA pure Operating Instructions 29 0229 97 AE 21 2 Safety instructions 2 1 Safety precautions Notices NOTICE Cleaning Keep the KTA pure instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the KTA pure instrument dry completely before use 22 KTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 2 Labels 2 2 Labels Introduction This section describes the safety labels that are attached to the AKTA pure instrument For information about marking of the computer equipment refer to the manufacturer s instructions AKTA pure instrument label The serial number of the AKTA pure instrumentis printed on the instrument labels located on the back of the instrument and below the pump tray on the front of the instrument XX XXXX KX XXXXX Sample Code no XXXXXXXX Voltage IXI XXX XXX
77. tavivescceeebuscute 54 COmectacColUM eaan nR e On on on NEC PEN asi Sho Precs Wedde spessi nnn T ERE EE eE aaa ne nr 5 6 Prepare for a run at cold room temperature on eeccesssesssssssecsseesseesseesssessssssssessecssecsseceseesseeeses KTA pure Operating Instructions 29 0229 97 AE ONO VI 12 13 23 26 29 30 32 55 44 45 46 Table of Contents WA R eases 109 OL PEVOU gna na Corn TET I oe OT 110 62 1 9 1711018 E SAMDE ananassae Pe nen eee EE 113 G3 StarntameNoa TUN esse ANE 116 OA MONO NETUN senesi E 118 65 PAGO GUA OCS sinnena eiino a E S E EE RE 119 E DN OOE E A E EO 123 B8 Relaence Nett ON sessami 126 Bd La PEC a e E T 127 82 Chemicalresbtonce gude nsensosiiirina E 130 83 Check hange ihe Node IDo a mMmodule arcsec cette cet eee 135 LaLe E E E EE E E EEE E A EE A E E 138 4 KTA pure Operating Instructions 29 0229 97 AE 1 Introduction 1 Introduction About this chapter This chapter contains important user information description of safety notices regula tory information and a general description of the intended use of AKTA pure In this chapter This chapter contains the following sections Section See page 1 1 About this manual 6 1 2 Important user information 7 1 3 Regulatory information 9 AKTA pure Operating Instructions 29 0229 97 AE 5 1 Introduction 1 1 About this manual 1 1 About this manual Purpose of the Operating Instructions The Operating Instructions provide
78. te AKTA pure Operating Instructions 29 0229 97 AE 107 5 Prepare the system for a run 5 6 Prepare for a run at cold room temperature 5 6 Prepare for a run at cold room temperature Introduction When using the instrument in a cold room or cold cabinet make sure to follow the pre cautions listed below Precautions concerning runs at cold room temperature NOTICE Avoid condensation If KTA pure is kept in a cold room cold cabinet or similar keep it switched on in order to avoid condensa tion NOTICE Avoid overheating If KTA pure is kept in a cold cabinet and the cold cabinet is switched off make sure to switch off KTA pure and keep the cold cabinet open to avoid overheating NOTICE Place the computer in room temperature If the KTA pure instru ment is placed in a cold room use a cold room compatible comput er or place the computer outside the cold room and use the Ether net cable delivered with the instrument to connect to the computer Note When the instrument is kept in a cold room it is important to tighten all tubing connectors also the inlet manifold connectors Otherwise air might get into the flow path Note Make sure that the instrument buffers and sample have had time to reach the ambient temperature When the instrument has reached the ambient temperature calibrate all pressure sensors 108 KTA pure Operating Instructions 29 0229 97 AE 6 Run
79. tenance About this chapter This chapter provides schedules for preventive maintenance that should be performed by the user of AKTA pure Regular maintenance is essential for reliable function and re sults Refer to AKTA pure User Manual for detailed instructions WARNING Always use appropriate personal protective equipment during op eration and maintenance of AKTA pure system Maintenance program An overview of the preventive maintenance to be performed on AKTA pure is outlined below See AKTA pure User Manual for detailed information about the maintenance procedures Maintenance is divided into e Weekly maintenance e Monthly maintenance e Bi annual maintenance e Maintenance when required WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation Periodic maintenance program The following periodic maintenance should be performed by the user of AKTA pure Interval Maintenance action Weekly Calibrate pressure monitors KTA pure Operating Instructions 29 0229 97 AE 123 7 Maintenance Interval Maintenance action Weekly Change pump rinsing solution Weekly Replace the inline filter in the Mixer Monthly Check the Flow restrictor Twice a year Clean the UV flow cell Maintenance when required The following maintenance sho
80. the parts and tubing of the pump piston rinsing system 7 Part Description Pump rinsing liquid tube holder top Pump rinsing liquid tube Pump rinsing liquid tube holder bottom Outlet tubing 5 Inlet tubing 74 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 2 Hardware installation 4 2 5 Prepare the pump rinsing system Prime the pump rinsing system Follow the instructions below to fill the pump piston rinsing system with rinsing solution See the tubing configuration of the rinsing system in the illustration above Step 1 Action Remove the pump rinsing liquid tube from the holder Fill the pump rinsing liquid tube with 50 ml of 20 ethanol Place the pump rinsing liquid tube back in the holder Insert the inlet tubing to the system pump piston rinsing system into the fluid in the rinsing solution tube Note Make sure that the inlet tubing reaches close to the bottom of the rinsing so lution tube Connect a 25 to 30 ml syringe to the outlet tubing of the system pump piston rinsing system Draw liquid slowly into the syringe AKTA pure Operating Instructions 29 0229 97 AE 75 4 Installation 4 2 Hardware installation 4 2 5 Prepare the pump rinsing system Step Action 6 Disconnect the syringe and discard its contents 7 Insert the outlet tubing into the fluid in the rinsing solution tube 8 Fill the rinsing solution tube so that the tube contains 50
81. to fill a sample loop Follow the instructions below to fill the sample loop with sample Step Action 1 Connect a suitable sample loop to the injection valve ports LoopF fill and LoopE empty 2 Fill a syringe with sample KTA pure Operating Instructions 29 0229 97 AE 113 6 Runamethod 6 2 Applying the sample Step Action 3 Connect the syringe to the injection valve port Syr 4 Open the System Control module 5 In the Process Picture e Click on the Injection valve and select Manual load Manual load Manual load 1 sye Manual load System pump waste Inject Sample pump load Direct inject Sample pump waste Result The injection valve switches to manual load position 6 Load sample into the sample loop To avoid sample loss due to siphoning leave the syringe in the port until the sample has been injected onto the column during the run Tip It is recommended to overload the loop to make sure that the loop is com pletely filled Excess of sample will leave the valve through port W1 114 AKTA pure Operating Instructions 29 0229 97 AE Sample application through a sample loop 6 Runa method 6 2 Applying the sample The method for how to apply a sample can be created beforehand see Section 6 3 Start a method run on page 116 During sample application the sample is automatically injected onto the column and the loop is then emptied and washed out using buffer from t
82. trument control panel displays a Slowly flashing light The run is interrupted immediately Data generated up to 10 seconds before the power failure can be recovered Note The UNICORN client may lose connection to the instrument during a temporary overload of the processor and display an error message This may appear as a computer failure The run continues and you can restart the UNICORN client to regain control No data will be lost Uninterruptible power supply UPS A UPS can prevent data loss during a power failure and allow time for a controlled shut down of AKTA pure For UPS power requirements see the system specifications in AKTA pure User Manual Remember to also take into account the specifications for the computer and monitor Refer to the manufacturers documentation 28 AKTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 4 Recycling information 2 4 Recycling information About this section This section describes the procedures for disposal and recycling of AKTA pure Decommissioning and disposal of the equipment When taking AKTA pure out of service e The equipment must be decontaminated e The components must be separated and recycled according to national and local environmental regulations CAUTION Always use appropriate personal protective equipment when decommissioning the equipment Disposal of electrical components Waste comprising electrical
83. u have a general understanding of how the computer and Microsoft Windows work e you understand the concepts of liquid chromatography e you have read and understood the Safety instructions chapter of these Operating Instructions e auser account has been created according to UNICORN Administration and Technical Manual AKTA pure Operating Instructions 29 0229 97 AE 7 1 Introduction 1 2 Important user information Safety notices This user documentation contains safety notices WARNING CAUTION and NOTICE concerning the safe use of the product See definitions below WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood gt NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A note is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures KTA pure Operating Instructions 29 0229 97 AE 1 Introduction 1 3 Regulatory information
84. uld be performed by the user of KTA pure when required Maintenance action Clean the instrument externally Perform System CIP Perform Column CIP Replace tubing and connectors Clean the Conductivity flow cell Calibrate the Conductivity monitor Calibrate the UV monitor Replace Mixer Replace O ring in Mixer Replace the UV flow cell Replace the Flow restrictor Replace inlet filters Clean the check valves Replace check valves Replace pump piston seals Replace pump pistons Replace pump rinsing system tubing Replace valve modules 124 AKTA pure Operating Instructions 29 0229 97 AE 7 Maintenance Maintenance action Wipe off excess oil from the pump head KTA pure Operating Instructions 29 0229 97 AE 125 8 Reference information 8 Reference information About this chapter This chapter lists the allowed environmental and operational ranges for AKTA pure Refer to AKTA pure Product Documentation for detailed technical specifications In this chapter This chapter contains the following sections Section See page 8 1 System specifications 127 8 2 Chemical resistance guide 130 8 3 Check Change the Node ID of a module 135 126 KTA pure Operating Instructions 29 0229 97 AE 8 Reference information 8 1 System specifications 8 1 System specifications System specifications Parameter System configuration Benchtop syste
85. unter clockwise about three quarters of a turn Draw liquid slowly into the syringe until the liquid reaches the pump 6 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents KTA pure Operating Instructions 29 0229 97 AE 83 4 Installation 4 5 Prime inlets and purge pump heads Step Action 7 Repeat steps 3 to 6 for each piece of inlet tubing that is to be used during the run Purge System pump B Follow the instruction below to purge both pump heads of System pump B Step Action 1 Make sure that the piece of waste tubing connected to the Injection valve port W1 is placed in a waste vessel 2 In the Process Picture e Click on the Injection valve and select System pump waste Result The Injection valve switches to waste position This is necessary to achieve a low back pressure during the purge procedure System pump waste Loope Loop valve Prec System pump By pass waste Cal TS Direct inject nject _ Sample pump Sample pump waste pump waste _ waste 84 KTA pure Operating Instructions 29 0229 97 AE 4 Installation 4 5 Prime inlets and purge pump heads Step Action 3 In the Process Picture e Click on the pumps e Set Conc B to 100 B 5 Pumps j 0 000 mi min Set flow rate A Conc B Pump wash A B Al wv Start pump A wash B1 v Start pump B wash e Click Set B Result Only System pump B
86. uption Emergency shutdown In an emergency situation stop the run by either pausing the run or switching off the instrument as described below If you want to pause the run press the Pause button on the Instrument control panel Note The buttons on the Instrument control panel may be locked This is an option available in the System settings or click the Pause icon in UNICORN t System Control File Edit view Manual System Tools Help Result All pumps in the instrument are stopped 26 AKTA pure Operating Instructions 29 0229 97 AE 2 Safety instructions 2 3 Emergency procedures If you want to switch offthe e press the Power switch to the 0 position or instrument e disconnect the power cord from the wall socket Result The run is interrupted immediately Note The sample and data may be lost as a result of switching off the power Power failure The result of a power failure depends on which unit is affected Power failure to will result in AKTA pure instrument e The run is interrupted immediately my e The data collected up to the time of the power failure is available in UNICORN KTA pure Operating Instructions 29 0229 97 AE 27 2 Safety instructions 2 3 Emergency procedures Power failure to will result in Computer The UNICORN computer shuts down The Power Communication indicator white on the Ins
87. ves resistant to the substances used Follow local and or national regulations for safe operation maintenance and decommissioning of the equipment CAUTION High pressure KTA pure operates under high pressure Wear protective glasses at all times gt gt PB CAUTION Risk of explosion Do not use Mixer chamber 15 ml in the low flow system The maximum pressure for Mixer chamber 15 ml is 5 MPa AKTA pure Operating Instructions 29 0229 97 AE Checklist 6 Runa method 6 1 Before you start Make sure that the system is correctly prepared Check that pressure range tity and volume placement and material The system is prepared according to the settings in the method to be run A suitable column has been selected for the application consider target protein and The buffer inlet tubing is immersed in correct buffer vessels consider solution iden All waste tubing is immersed in appropriate waste vessels consider vessel size e No tubing is twisted and the flow path is free from leakage Hold pause or stop the run At the end of a method the run stops automatically All pumps stop and an acoustic end signal sounds and End is displayed in the Run Log To interrupt a method during a run you may use the Hold Pause or End icons in System Control A held or paused method run can be resumed by using the Continue icon See the instructions in the table below If you want to temporaril
88. y hold the method with current flow rate and valve positions sustained then click the Hold icon temporarily pause the method and stop all pumps click the Pause icon m resume for example a held or paused method run click the Continue icon gt Note An ended method cannot be continued AKTA pure Operating Instructions 29 0229 97 AE 111 6 Runa method 6 1 Before you start If you want to permanently end the run click the End icon Note When ending a method run in advance it is possible to save the partial result Warnings concerning use of hazardous substances CAUTION Hazardous chemicals during run When using hazardous chemi cals run System CIP and Column CIP to flush the entire system tubing with distilled water before service and maintenance 112 AKTA pure Operating Instructions 29 0229 97 AE 6 Runa method 6 2 Applying the sample 6 2 Applying the sample Introduction A number of different sample application techniques are available This section describes Sample application using a syringe to manually fill a s loop The two stages of the sample application are described in the table below For detailed instructions and information regarding the different sample applications techniques see AKTA pure System Handbook Stage Description Load The sample loop is filled with sample Inject The sample is injected onto the column How
89. you with the instructions needed to install operate and maintain AKTA pure in a safe way Typographical conventions Software items are identified in the text by bold italic text A colon separates items in a group thus Flowpath Injection valve refers to the Injection valve item in the Flowpath group Hardware items are identified in the text by bold text for example the Power button 6 AKTA pure Operating Instructions 29 0229 97 AE 1 Introduction 1 2 Important user information 1 2 Important user information Read this before operating AKTA pure All users must read the entire AKTA pure Operating Instructions before installing operating or maintaining the instrument Always keep the AKTA pure Operating Instructions at hand when operating AKTA pure Do not operate AKTA pure in any other way than described in the Operating Instructions Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use AKTA pure is intended for the purification of bio molecules in particular proteins for re search purposes It is intended to be used by trained laboratory staff members in research laboratories at departments within academia and industry AKTA pure shall not be used in any clinical procedures or for diagnostic purposes Prerequisites In order to follow this manual and use the system in the manner it is intended it is im portant that e yo
90. your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 0229 97 AE 01 2015 a2028

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