A2-OK Horizontal System - User Manual
Contents
1. _ EEC o G CU CT E pnm COCO ESTE E ee Penner s s CEIC s semen _ CON UN je Bee EEC 5 _ fe Hydrogen peroxide 28 solution fe Te a s s EE o E ESO CT Beas meme Posen u O CO This list does not include all possible chemical incompatibilities and safe compounds products should be cleaned with warm water a mild detergent such as Alconox and can also be exposed to a mild bleach solution 10 1 In addition RNAse removal products are also safe for acrylic Contact Technical Services with any questions Thermo Scientific Horizontal System 7 3 Section 8 Optional Equipment Leveling Platform The three point leveling platform Model B LP ensures a flat casting and running surface the platform is 46cm x 36cm and is large enough to fit most applications One bubble level BBL 1 is included ae t Contact Technical Services to order replacement parts Table 8 2 Replacement Parts Cate No see sehen Description PSU es an ar Power Supply Leads BIAUVT sardina Gasketed UVT gel tray BIACS gu Multiple Gel Caster A Un Gel Tray Holder 1 Replacement Gaskets2. Section 9 Warranty Information 1006 OSI cr 6 0 es JO J0jnqujsip Jno YSN 99 episino eroeds pue sonas eoueuejureuJ AjueJeM Juswd nba uo suonsenb peal 9J 9M EILF EJE OVZ L JO YSN 009 1e jueugedeq 2 eseejd s sones y pue uonejejsur 3ueuudinboe uononuajsui paud yuswd nba aus SAISUBYSJGLUOD yym day Apes 51 220 INOA Sjonpoud sso JO 150 0 moyym jenuenbesuoo Jo Aue eq jou jeus 94 AlddV TIVHS YVINOILYVd V SSANLIS ALNIGVLINVHOYSW SSILNVYYVM ON WHO N3 LLIHM YSHLSHM S3ILNVHSHVM H3HLO TIV 30 GNV 3A180719X3 SI ALNVYYVM SIHL uoneunsep go y peddiys sued pue pied aBejsod peuunjeJ eq sued tuondo 5 Jo Aue jo
3. 1 pair Thermo Scientific Horizontal System 8 1 Section 9 Warranty Information ERENODEL 1006 OSI 216 0 uoneuuoJul Jojnqujsip 890 Jno JOeNUOD YSN eui episino elo eds pue sonas AjueueM Juswd nba uo suonsenb noA Jamsue peaJ 9J 9M 292 7 272 2 pue YSN 158y 2 y 008 1 1e jueuniedog jesiuyos peuinbe s sones 4 pue Juswd nba uononuajsui yuswd nba uoneuuojul aus SAISUBYSJGLUOD uj day Apes s sejes 220 INOA sjonpoJd sso 5 150 jenuenbesuoo Jo Aue JO aq 100 JeEYS AlddV TIVHS 3ISOdYINdA YVINOILYVd SSANLIS HO ALITISVLNVHOUYFN S3LENVSHSHVM ON WHO N3 LLIHM YSHLSHM SSILNVYYVM H3HLO TIV 30 GNV 3A180719X3 SI ALNVYYVM SIHL uoneunsep peddiys sued 1ueujeoe doaJ pue pied aBejsod ouueu L eq sued 2 uondo s ouueu Jo jueuodu
4. 9AID 5 eoiuuo9 eu jueJem y y ay jou siy Jepun Jo sued JO SIU sjexseb pue sia ssejD sway ejqepuedx3 Aue jo Jod JOUd pue uoneuiuuJejep JO pajoejuo9 eq juawuedag sasas eoluyos 94 ueuleeJ6e siy Aq zou SI pue Bundeoxe 5 1e peoejdoiJ Jo ui oj ue oJd sued sujuouu 1814 ayy Buung Jsumo juenbesqns Aue uon 98 01d JUeJem ay si juaudinba noA y Pays ojur jueJem ay os Buiddius siu peddius si ajep eui syyuow OM SHES poueg TWNOILVNYSLNI SLINAOYd IMO 2131133195 514 1 Thermo Scientific Horizontal System 9 2 Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio
5. 1 is an internationally accepted electrical safety standard for laboratory instruments Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual 2012 Thermo Fisher Scientific All rights reserved i Horizontal System Thermo Scientific Important operating and or maintenance instructions Read the accompanying text carefully gt gt gt Potential electrical hazards Only qualified persons should perform procedures associated with this symbol re gt Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 ERA This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted
6. 45750 United States www thermofisher com ThermoFisher SCIENTIFIC
7. at a time and lining up the bubble in the level with the center circle Place the gel tray holder in the buffer chamber add buffer remove combs load samples and run the gel Thermo Scientific Horizontal System 2 1 Section 2 Setting Up 2 2 Horizontal System Preparing the gel Using electrophoresis grade agarose and compatible electrophoresis buffer the gel may be prepared in various ways The percentage of agarose and the electrophoretic buffer used is determined by the size of the samples to be separated and further recovery of the samples The agarose and buffer are mixed and heated over a heat source in a microwave oven or in an autoclave until the agarose is completely dissolved The prepared gel then must be cooled to below 60 before casting to avoid warping the UVT gel tray due to excessive heat If numerous gels are to be run in one day a large volume of gel may be prepared and placed in a covered bottle stored between 40 60 in a water bath This provides a ready gel supply in a warm liquid form that will solidify quickly when gels are cast Pour or pipet warm agarose lt 60 Immediately after pouring insert the desired comb or combs into the comb slots to form the sample wells If only a small portion of gel is required for proper sample separation multiple combs may be used to run 1 or 2 sets of equal distance samples simultaneously expanding the number of samples per gel that may be run Repeat for up to 6 g
8. gel or gel tray in the ing in the wells or diffusing into funit The platinum wires on both sides of the unit should pro the gel duce small bubbles as the current passes through If a complete circuit does not exist there will be few to no bubbles Contact Technical Services to schedule a repair Always make sure to allow the gel to solidify completely before moving the gel tray unit or removing the comb To avoid damage to the sample wells gently rock the comb back and When the comb is removed from forth lightly to loosen then slowly pull the comb straight up out the gel some sample wells are of the gel tray This rocking helps to avoid suction as the comb ripped and damaged is removed Alternatively once casting is complete simply sub merging the gel with running buffer will help loosen the comb Using a higher percentage of agarose that forms a tighter gel matrix may remedy this problem as well The volume of running buffer used to submerge the gel should only be between 3 5mm over the gel surface Gel should be completely submerged to avoid the gel from drying out which The gel seems to run slower can smear the bands and possibly melt the gel s due to over under the usual running conditions heating If excessive running buffer is added the mobility of the DNA decreases and band distortion may result Excess buffer causes heat to build up and buffer condensation inside the unit may result 6 2 Horizontal System
9. that include treatment with 0 1 Diethyl Pyrocarbonate DEPC treated water and soaking in diluted bleach This electrophoresis system should never be autoclaved baked or placed in a microwave Warning DEPC is suspected to be a carcinogen and should be handled with care To order RNase Away contact Technical Services Part Description ODDO 23 edi E ee 250ml botte 7002 abate p ERAI EE 475ml spray botte POIS SS ro Dv teda 1 liter bottle A ade en en 4 liter bottle Rnase Away is a registered trademark of Molecular BioProducts Thermo Scientific Horizontal System 74 Section 7 Care and Cleaning Care of Acrylic The following chemical compatibility chart is supplied for the convenience of our customers Although acrylic is compatible with most solvents and solutions found in the biochemical laboratory some solvents can cause substantial damage Keep this chart handy to avoid harm to your apparatus by the use of an inappropriate solvent Codes S Safe No effect except possibly some staining A Attacked Slight attack by or absorption of the liquid Slight crazing or swelling but acrylic has retained most of its strength U Unsatisfactory Softened swollen slowly dissolved D Dissolved In seven days or less 7 2 Horizontal System Thermo Scientific Section 7 Care and Cleaning Table 5 1 Chemical Compatibility for Acrylic Based Products PAS E omas LN
10. Horizontal System Model 2 0 Operating and Maintenance Manual 7219011 Rev 0 Visit us online to register your warranty www thermoscientific com warranty 6 Owl Separation Systems MANUAL NUMBER 7219011 0 4 13 12 Transfer to Marietta was 11 2002 CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific Horizontal System i Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance Caution All internal adjustments and maintenance must be performed by qualified service personnel Warning To avoid the risk of personal shock always disconnect the gel box from the power supply Further the power supply must be equipped with a shut down on disconnect circuit Do not move the unit unless the power source to the unit has been disconnected Statement of Proper Use Use this product only for its intended purpose as described in this manual Do not use this product if the power leads are damaged or if any of its surfaces are cracked Warning Running conditions for this unit should not exceed the name plate readings found on the lower buffer chamber This Owl System is designed to meet IEC 1010 1 safety standards IEC 1010
11. Thermo Scientific Thermo Scientific Section 6 Troubleshooting Additional Sources for Reference Maniatis T E Fritsch and Sambrook Molecular Cloning A Laboratory Manual Cold Spring Harbor Laboratory Cold Spring Harbor NY Short Protocols in Molecular Biology A Compendium of Methods from Current Protocols in Molecular Biology Edited by Fredrick M Ausubel et al Adams D and R Ogden Electrophoresis in Agarose and Acrylamide Gels Methods in Enzymology Vol 152 1987 Academic Press Inc Fotador U Simultaneous Use of Standard and Low Melting Agarose for the Separation and Isolation of DNA by Electrophoresis BioTechniques Vol 10 No 2 1991 Boots S Gel Electrophoresis of DNA Analytical Chemistry Vol 61 No 8 April 15 1989 Horizontal System 6 3 Section 7 Gare and Cleaning Caution Organic solvents cause acrylic to craze or crack Clean all acrylic systems with warm water and a mild detergent Do not use ethanol or other organic solvents to clean these products Do not autoclave bake or microwave your unit Temperatures over 50 can damage the acrylic Note If an RNase free electrophoresis system is desired there are various methods to rid the system of RNA contamination For fast and easy decontamination use RNase Away Spray wipe or soak labware with RNase Away then wipe or rinse the surface clean it instantly eliminates RNase RNase Away eliminates the old methods
12. e 40 mM Tris acetate 57 1 ml glacial acetic acid 1 mM EDTA 18 61 g Na EDTA 2H20 MW 372 24 Distilled H2O to 1 liter final volume TBE Tris borate with EDTA 89mM Tris base 89mM boric acid 2mM EDTA 10X stock solution 1X working solution 108 g Iris base 89 mM Tris base 55 g boric acid 89 mM boric acid 7 44g Na2EDTA 2820 MW 372 24 2 mM EDTA or 40 ml 0 5 M EDTA pH 8 0 Distilled H2O to 1 liter final volume Do not adjust pH Buffer Suggested Uses and Comments TAE Buffer Use when DNA is to be recovered For electrophoresis of large gt 20kb DNA Applications requiring high resolution Has low ionic strength and low buffering capacity recirculation may be necessary for long runs gt 4hrs TBE Buffer For electrophoresis of small 1kb DNA Better resolution of small lt 1kb DNA Decreased DNA mobility High ionic strength and high buffering capacity no recirculation needed for extended run times TBE buffer reacts with the agarose making smaller pores and a tighter matrix This reduces broadening of the DNA bands for sharper resolution Horizontal System 5 3 Section 5 Technical Tips 5 4 Horizontal System Ethidium Bromide Ethidium bromide is ideal for the flurometric detection of nucleic acids in gel electrophoresis The addition of ethidium bromide to both the prepared gel and running buffer is a convenient way to monitor separation and keep a photographic log of gel runs Ethidium B
13. el tray from the external casting tray and place the tray on the lab bench Carefully remove the sample combs by tapping and lifting straight up Samples mixed with loading buffer that does not contain dye may be easier to load dry especially in larger gel units to avoid cross contamination After loading all sample lanes place the gel tray into the gel tray holder in the running position with the gel edges facing out toward the buffer chambers and slowly fill as described in Step 2 It is wise to always run a sample lane of a known standard ladder to determine concentration and size of separated fragments after the gel run and to aid in photo documentation and analysis Note It is sometimes easier to load the sample wells dry before placing the gel tray into the running position 5 6 Carefully slide the Supersafe lid with attached power supply leads onto the unit This will connect the power cords to the banana plug electrodes and complete the circuit Plug other end of the power cords into appropriate power supply Turn on power supply see Recommended Running Conditions Carefully monitor the gel run to avoid samples running into the path of another set of samples Thermo Scientific Thermo Scientific Section 4 Finishing Up 1 When the gel run is complete and tracking dye has migrated as far through the gel as desired or to the end of the gel turn off the power supply and slide off the lid to disconnect from the po
14. els 3 per external multiple gel caster Thermo Scientific Section3 Using the System 1 Once the gel s is completely solidified lift the gel tray 5 out of the external gel caster Place the gel tray s onto the gel tray holder Place the gel tray holder in the buffer chamber in the running position The running position exposes the open ends of the agarose to the buffer Standard agarose should solidify completely in about Figure 3 1 Place Gel Tray 30 minutes 2 Pour enough compatible running buffer into the unit to fill the chamber and completely cover and submerge the gel Correct buffer level is clearly marked on the units side wall as FILL LINE See Recommended Running Conditions for approximate buffer volumes needed for your unit Too little buffer may cause the gel s to dry out during the run while excess buffer may decrease DNA mobility and cause band distortion Figure 3 2 Place Gel Tray Holder 3 Carefully remove the comb or combs by tapping lightly to loosen and slowly lifting straight up out of the gel tray to avoid damage to the wells Thermo Scientific Horizontal System 3 1 Section 3 Using the System 3 2 Horizontal System Load prepared samples into the wells Samples should be mixed with a sample loading buffer giving weight to the samples so that they drop evenly into the wells and containing tracking dyes to monitor the gel run After the gel solidifies remove the g
15. etting ee 2 1 Preparing ates bathe aes Luces da 2 2 Using the System 3 1 Finishing Up 2 3 en 4 1 Specifications and Recommended Running Conditions 4 1 Technical en Bes 5 1 COD ASA 5 1 Reagent Information o piede DR e ES 5 2 Troubleshooting u re 6 1 and 7 1 Optional 8 1 Replacement Parts 2s durs el Me a 8 1 Horizontal System V Thermo Scientific Section 1 General Information The Opossum Multiple Gel system is ideal for teaching labs or labs that need to run multiple gels simultaneously Up to six minigels 7cmW x 8cmL can be run on this versatile system Along with optional combs the Opossum can screen up to 144 samples at once 120 with supplied combs EasyCast casting design allows the casting of gels without tape or plugs UV transmissible gel trays may be placed directly on the transilluminator for photodocumentation Before starting unpack the unit and inventory your order If any parts are missing contact Technical Services immediately Reference the order or catalog number on your invoice and check the corresponding parts list Table 1 1 Parts List Buffer Chamber Combs 10 well comb Double Sided 1 0 1 5mm Thick 12 Super Safe Lid with a
16. lowing the gel run without the need for additonal staining If this is not added then following the gel run the gel may also be soaked in a concentrated ethidium bromide solution and rinsed for the same visualization The ethidium bromide is added to the gel after heating and the electrophoresis buffer at a concentration of 0 5ug ml Warning Ethidium bromide is a potential carcinogen Care in handling the powder and stock solution must be taken Always wear gloves when handling the powder solutions and all gels that contain any amount of ethidium bromide A Horizontal System 4 1 Section5 Technical Tips Standard Standard e 1 0mm C and 1 5mm D thickness for all models Double Sided Double Sided Molded Combs combine 1 0mm and 1 5mm tooth thickness on one comb Double Sided Preparative One long well and one marker Preparative Wall Comb See page 33 for details D p Wall Comb Multi Load Comb F or use with 8 channel pipet Custom Combs Multi Load Comb Call Technical Services for more information Thermo Scientific Horizontal System 5 1 Section 5 Technical Tips 5 2 Reagent Information Horizontal System Chart A Mobility range of DNA in different percentage agarose gels Agarose w v Approximate range of separated DNA fragments kb inne Gee 60 5 e e 30 1 v 12 t
17. o 0 8 bM 10 to 0 5 A a a qe pata eds 7 to 0 3 A els orn bate ts 4 to 0 2 DO shared TP 3 to 0 1 Di On NOUS lt 0 1 It should be noted an increased agarose gives better separation of small fragments and also bands very close together that tend to be more difficult to separate visualize and photograph A specialty agarose product formulated to increase resolution of low molecular mass samples may also be used Example A good mid range gel percentage would be 0 7 or 0 7g agarose in 100mls electrophoresis buffer TBE or TAE following heating and dissolving the agarose 10ul of ethidium bromide stock solution 5mg ml is added The gel would be run with compatible electrophoretic running buffer 1X TBE or TAE that also contained ethidium bromide 1 liter of the running buffer would contain 100ul of this 5mg ml ethidium bromide stock solution Thermo Scientific Thermo Scientific Section 5 Technical Tips Chart B Preparation amp Properties of TAE and TBE Electrophoresis Buffer Systems These buffers are used because they both have a basic pH which gives the phosphate group of the DNA a net negative charge allowing migration of the DNA toward the positive anode in the electrophoresis chamber TAE Tris acetate with EDTA 40mM Tris base 40mM acetic acid 1mM EDTA 50X stock solution pH 8 5 1X working solution 242 g Tris bas
18. on or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv Horizontal System Thermo Scientific Thermo Scientific Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 7 Section 8 Table of Contents General Information 1 1 S
19. ove gasket and reseat by smooth ing out gently with your thumb from one end to the other Gasket material may have a tendency to absorb salts from the running buffer After each use rinse the end gates under warm running water to bring back spongelike consistence of the gas ket material Gaskets may eventually become brittle with fre quent use Contact Technical Services to purchase replacement gaskets Agarose leaks into chamber when casting the gel Check to be sure that the unit is properly leveled for casting and running the gel by using the thumbscrews on the base Bands seem to be running at Thumbscrews should be adjusted until the bubble in the level an angle lines up with the levels center circle Always center the gel tray holder on the platform and cool the agarose to below 60 before pouring to avoid warping the UVT gel tray s Check that the platinum electrode wire is intact running flat and evenly across the outer corners and up the side to the junction of the banana plug area This problem could also be Samples seem to be running caused by regular casting with very hot agarose gel 60 unevenly in certain areas which may damage the gel tray over time Always cool the melted agarose to below 600 before casting to avoid warping the UVT gel tray s Warping the UVT gel tray will cause all subsequent gels to be cast unevenly Gels should be no more than 5mm thick and allowed to solidify completely before running For s
20. romide is prepared as 10mg ml in distilled water and used as a stock working solution of 5 0ug ml in the electrophoresis buffer and gel Mix ethidium bromide powder or tablet thoroughly into solution checking for any precipitate and store at room temperature protected from light Chart C Amount of Agarose to prepare All approximate well volumes given in the chart below are based on a gel of 0 5cm thickness Gel volume is determined by the following formula and may be adjusted according to need or preference gel width cm x gel length cm x gel thickness cm ml of agarose ram Agarose Volume in ml per gel thickness in cm Model Be ASES Agarose Gel Loading Buffer Samples are prepared and combine with gel loading buffer before applying to the prepared gel Sample buffer usually contains similar components to the running buffer dyes for visibility and glycerol to provide some weight to the samples This increased sample density and color allows easy visualization of the samples and ensures samples load evenly into the wells and do not float out during loading Dyes also migrate toward the anode end of the electrophoresis chamber at predictable rates allowing the gel run to be monitored The most commonly used loading buffer is glycerol bromophenol blue and xylene cyanol Thermo Scientific Section 6 Troubleshooting m qm Check to see if the gasket is correctly seated in groove and even all the way around Rem
21. tandard agarose this would be about 30 minutes if low melting point agarose is Samples do not band sharply used it may be necessary to completely solidify gels at a and appear diffuse in the gel cooler temperature in the refrigerator or cold room Gels should be submerged in 3 bmm of buffer to avoid gel dry out but excess buffer gt 5mm can cause decreased DNA mobility and band distortion Thermo Scientific Horizontal System 6 1 Section 6 Troubleshooting m qm Always follow the proper procedure for preparing the agarose product according to the manufacturers instructions When preparing the agarose be sure all the agarose powder is in solution before heating In general add powdered agarose to distilled water and swirl to mix Make sure all the powder is equally wet to ensure proper melting Heat in a microwave oven boiling water bath or hot plate with occasional swirling to melt and mix completely Cool agarose liquid to below 60 and cast Note Gel should be cast no thicker than bmm to avoid fuzzy banding High percentage gels may thicken and solidify rapidly and should be cast while still a liquid Bands are not sharp clear and even Check that a complete power circuit is achieved between the unit and the power supply Platinum wire and banana plugs Samples are not moving as should be intact To test simply fill the unit with running buffer expected through the gel remain and attach to the power supply without a
22. ttached power supply leads Multiple Gel Caster 2 Gel Tray Holder UV Transmissible gel trays 6 Horizontal System 1 1 Section 1 General Information 1 2 Horizontal System Power Supply Leads Super Safe Lid Gasket 12 UVT Gel Tray 6 SX Gel Tray Holder Multiple Gel Buffer Caster 2 Chamber Double Sided 20 181 Comb 12 ace Thick Bubble Level Figure 1 1 Exploded Parts Diagram Table 1 1 Specifications Gel size Siri 7cmW 8cmL Running Buffer Volume 2 3L Footprint 28cm W x 37 5cm L x 10cm H Thermo Scientific Section 2 Setting Up 1 For shipping and convenient storage the gel tray is packaged inside the unit with the gasketed end gates in position upon arrival Lift the casting tray out of the buffer chamber 2 cast gels place the gel tray s into the external multiple gel caster The gasketed sides of the UVT gel tray form a leak proof seal along the side of the gel caster Figure 2 1 Casting Tray 3 Prepare and pour warm gt 60 agarose solution onto UVT gel tray Place comb s into slots provided on walls of UVT gel tray X amp 12 EA e Y po A Figure 2 2 Pour Agarose Figure 2 3 Place Combs 4 One the gel has solidified place the UVT gel tray 5 into the gel tray holder 5 Level the casting chamber using the thumbscrews on each side of the unit by slowly turning one thumbscrew
23. uoo Aue jo unya JO an b 5 eu jueJem y Juswd nba ay jou jUeJem siy Jepun juaudinba Jo sued Jo JO JU amp JJeM SIU pepnjoxe sjexseb pue say 552 6 ejqepuedx3 Aue jo eoue oj Joud pue jueJem snu juauyedag sadas esluyoa 941 siy jou SI pue peoejdoJ Jo ue oJd syed xis Auiu 3814 ayy BUNG juenbesqns jueJem 99 si jueuudinbe JNOA y joaye jueJem ay os 5 smoje siu uno peddius si 3ueuudinbe ay syaam Om 5 AueJeM 941 VSN SLINAOYd IMO 2131133195 514 OINH3HL 9 1 Horizontal System Thermo Scientific
24. wer source Carefully remove the tray s containing the gel wear gloves if ethidium bromide is present The UV Transmissible UVT gel tray makes visualization and photography with a UV light source easy without the need to remove the gel from the tray 2 The gel box should be rinsed under warm running water after each use including the UVT gel tray s Rinsing the UVT gel tray s will avoid salt build up in the gasket material from the electrophoretic running buffer extending the gasket life and ensuring leak free gel casting An RNase DNase decontaminate may be used This electrophoresis system must never be autoclaved baked or microwaved Specifications and Recommended Running Conditions ELE td lao 7cmW x 8cmL B ffet Capacity una Ks ea 2 31 There are various types of agarose commerically available that may be used Besides standard ultra pure electrophoresis grade agarose there are also numerous low melting point products for easy sample recovery as well as speciality products formulated for specific uses to separate recover very small or very large fragments etc visualize and photograph the samples after the gel run for a permanent record the gel may be stained during or following the run with a variety of stains The most common stain for DNA is ethidium bromide Ethidium bromide may be added directly to the gel and running buffer to quickly and easily visualize and photograph the separated fragments fol
25. with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo s compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermofisher com Y Always use the proper protective equipment clothing gloves goggles etc Y Always dissipate extreme cold or heat and wear protective clothing Y Always follow good hygiene practices Y Each individual is responsible for his or her own safety Thermo Scientific Horizontal System iii Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operati
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