PRECELLYS® Evolution SAMPLE PREPARATION & CELL LYSIS
Contents
1. INSTALLATION 4 2 Installation and connection to power supply 1 Remove the protective foam located around the metallic support ring and reposition the containment seal see 7 2 2 ARNING b Keep this protective foam in a safe place as it must be put back in place before shipping the unit If the unit is returned to the distributor or manufacturer without this protection in place the warranty will be cancelled The containment seal must be positioned in compliance with the instructions provided in this user manual in order to ensure optimum operator protection The containment seal is indeed a key element in ensuring the containment of all electrical parts of the equipment and protecting them against accidental liquid 2 Position the toric joint in place see 5 7 2 1 ARNING B If the voltage supplied is not 3 Check the coherence of the technical specifications provided in the same as that indicated on this user manual with the voltage delivered by your local electric the equipment the electronic power supply see 5 2 1 components of the latter may be damaged J 4 Ensure that the air intake vents and the base of the casing are unobstructed the air extraction vent is located beneath the unit A ARNING Allow a space of at least 10 foot cm around the air intake and extraction vents air outlet ARNING b 5 Co2. Before transporting the equipment it is essential to 1 Reposition the protective foam to retain the tube holder in place 2 Close the lid 3 Use the original packing components 3 2 Storage The unit must be stored in a dry area at a temperature ranging from 0 to 50 4 Installation 4 1 Unpacking 1 Check the contents of the box in order to ensure that all of the following items are present 1 user manual French English 1 Precellys Evolution including one 2ml indented plate one 2ml tube holder one toric joint and one containment seal 1 power cable 1 spare toric joint 1 spare fuse 1 QC report 1 ECcertificate of conformity 1 manufacturer s inspection certificate In the event that any of these items are missing please contact your local distributor immediately A plug adapter or compatible power cable not included will be required for countries other than the following Europe United Kingdom USA Switzerland and Australia 2 Carefully remove Precellys Evolution weight 27 2 kg from its box and place it on a clean flat and stable work surface the 4 feet are height adjustable to ensure optimum stability of the equipment 3 Inspect the equipment carefully and report any damage to the carrier immediately 4 Keep the box the protective foam and the documentation in a safe place all will be required in the event that the equipment needs to be returned
3. lus The Science of Lysing peolabz a VWR company USER MANUAL MANUEL UTILISATEUR PRECELLYS Evolution SUPER Homogenizer SAMPLE PREPARATION amp CELL LYSIS tin TECHNOLOGIES PRECELLYS Evolution USER MANUAL Produced by BERTIN TECHNOLOG ES Parc d activit s du Pas du Lac 10 bis avenue Amp re BP 284 78053 Saint Quentin en Yvelines Cedex France Phone 33 0 1 39 3061 60 Email info precellys com Reference 002520 80 00002 B bertin Page TECHNOLOGIES SOMMAIRE INTRODUCTION 1 1 Safety instructions and recommendations 1 2 VVarranty 1 3 Equipment references 1 4 Manufacturer s address 1 5 Technical support p DESCRIPTION OF PRECELLYS EVOLUTION 2 1 Product overview 2 2 Tube motion 23 Control panel 2 4 MMI menus 2 5 Programming 2 6 Equipment specifications di TRANSPORT AND STORAGE 3 1 Transport 3 2 Storage 4 INSTALLATION 4 1 Unpacking 4 2 Installation and connection to power supply INSTRUCTIONS FOR USE 5 1 Preparing samples 5 2 Launching a lysing protocol 5 3 Lysing kit use by dates 6 ALARM MESSAGES 7 7 1 Troubleshooting guide 7 2 Replacement of spare parts 8 CLEANING 8 DECONTAMINATION 8 1 Recommendations 8 2 Example decontamination procedure 9 REPAIR AND DISPOSAL 9 1 Repair 9 2 Disposal 10 ANNEX 1 Page 3 bertin TECHNOLOGIES PRECE
4. 2 mL tube holder SP02520 820 NC001 0 1 year or when damaged Essential for maintaining tubes in place during homogenization 7 mL indented plate 7 mL tube holder SP02520 810 NC002 0 SP02520 820 NC002 0 1 year or when damaged 1 year or when damaged Essential for maintaining tubes in place during homogenization Essential for maintaining tubes in place during homogenization 15 mL indented plate SP02520 810 NC003 0 1 year or when damaged Essential for maintaining tubes in place during homogenization 15 mL tube holder SP02520 820 NC003 0 1 year or when damaged Essential for maintaining tubes in place during homogenization Toric joint SP02520 810 NC005 0 6 months or when damaged Essential for maintaining tubes in place during homogenization Containment seal SP02520 810 NC004 0 1 year or vvhen damaged Essential for maintaining tubes in place during homogenization Fuse SP02520 810 NC006 0 VVhen blovvn Anti rotation kit SP02520 810 NC007 0 1 year or when damaged Essential for maintaining tubes in place during homogenization VARNING Potentially dangerous voltage exists inside the instrument n order to ensure user safety in particular during cleaning and or decontamination procedures the containment seal must not be damaged in any vvay perforation tear etc Never set the unit at an angle Precellys Evo
5. Faulty cooling system 1 Switch off the unit 2 Contact Bertin Technologies technical support team No display on the screen No power supply to 1 Check the mains voltage mains plug 2 Ensure that the voltage of the unit matches that delivered by the mains power supply 3 Ensure that the unit is plugged in properly Faulty fuse Replace the fuse see 7 2 3 Faulty display system 1 Switch off the unit 2 Contact Bertin Technologies technical support team One or more tubes are not tight The cap has not been properly screwed on or the tube is faulty The tube used is not a Precellys tube The limits of use have not been met If a dangerous or potentially dangerous product is contained in the tube apply the proper decontamination procedure see 8 Povver cut For safety purposes the indented plate is held dovvn by vacuum pressure Reconnect the power supply in order to remove the indented plate bertin TECHNOLOGIES Page 20 MAINTENANCE 7 2 Replacement of spare parts This paragraph specifies the maintenance actions that users must perform on a regular basis in order to ensure the continuous good working order of Precellys Evolution VVearing part Reference Frequency of replacement Why 2 mL indented plate SP02520 810 NC001 0 1 year or when damaged Essential for maintaining tubes in place during homogenization
6. etc that must not be exceeded for the good preparation of biological samples Use by dates for our lysing kits are available on our web site www precellys com 1 1 2 Risk of electric shock Although this equipment is fully insulated and earthed it is important for all users to be aware of the potential hazard of using liquid close to a power supply If any liquids are spilled disconnect immediately the equipment from the main power supply remove the power cord from the AC power input on the rear panel and clean the equipment and the surrounding area Do not reconnect the equipment until it has been fully inspected INTRODUCTION 1 1 3 Abnormal operation Operating this equipment in any other way than that described in this user manual may impair the protection of the unit Never set the unit at an angle Precellys Evolution must always be used when evenly balanced on its 4 feet Failure to comply with this instruction may result in damage to the internal components or in breakage of the plastic casing High voltage vvarning do not handle or operate the unit if the casing is vvholly or partially removed or is damaged Do not use or handle the unit vvhen the protective earth is disconnected Do not install unauthorized cards components or accessories as this may impair the safety of the unit and will render the vvarranty void Ensure that the voltage indicated on the rear panel of the unit matches that
7. rise Page 9 bertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL DESCRIPTION 2 3 Control panel The screen immediately lights up when Precellys Evolution is switched on and displays the main menu after a few seconds once the system has booted up Preceliys Evolution start stop N 660 bertin Page10 TECHNOLOGIES DESCRIPTION 2 4 MMI menus A few seconds after switching on the equipment the main menu will be displayed enabling access to the different menus of the menu tree described below Main menu Sub menus or Description possible actions Start Choose a pre set Press the Left Right buttons to select from the sliding bar at protocol the bottom of the screen Adjust a protocol setting solely in Work mode 1 Navigate in the screen using the Up Down buttons 2 Select the setting to adjust by pressing the OK button 3 Adjust the value of the setting using the Up Down buttons 4 Validate the new value by pressing the OK button Launch a lysing protocol Press the Start button When the protocol has ended press the Stop button to go back to the previous menu System settings Choose language Change the language of the MMI by pressing the OK button Set date Adjust the date and time by pressing on the OK button and then using the Up Down
8. LLYS Evolution INTRODUCTION This user manual includes all necessary information regarding the unpacking operation and maintenance of the Precellys Evolution homogenizer The technical specifications of this product and all the information contained in this manual are liable to alteration without prior notice bertin TECHNOLOGIES Page 4 USER MANUAL 1 Introduction 1 1 Safety instructions and recommendations This user manual must be read carefully by all users prior to operating the Precellys Evolution lyser homogenizer If there is any doubt or concern about the safety of the equipment please contact your local distributor Bertin Technologies shall not be liable for any damage or injury that may occur as a result of operating the instrument in a different way to that stated in this document 1 1 1 Use of lysing kits Depending on the parameters of the Precellys Evolution speed number of cycles cycle duration waiting time between two cycles excessive overheating of the lysing tubes may lead to their sudden degradation In order to ensure the good working order of this Precellys Evoution equipment users are strongly recommended to use the Lysing Kits produced by Bertin Technologies and to comply with the use by dates specified for each lysing kit These use by dates specify the maximum parameter levels e g speed number of cycles cycle duration waiting time between two cycles
9. age 17 bertin TECHNOLOGIES PRECELLYS Evolution INSTRUCTIONS A ARNING B Ensure that the system has stopped completely before opening the lid To prevent the equipment from overheating a rest period of 2 to 5 minutes between two lysing protocols is strongly J ARNING Use of lysing kits beyond their use by date may result in sudden damage to tubes bertin TECHNOLOGIES Page 18 USER MANUAL 5 2 6 End of a lysing protocol At the end of a lysing protocol the system returns to the Start menu The vacuum created beneath the indented plate will be released a few seconds after the system has shut down the indented plate cannot be removed during this short period 5 2 7 Interruption of lysing cycle by the operator Operators can interrupt a lysing protocol at any moment by pressing the Stop button When a protocol is interrupted by the operator the time remaining is displayed Pushing the button Stop or Start allows the system to go back to the Start menu The vacuum created beneath the indented plate will be released a few seconds after the system has shut down the indented plate cannot be removed during this short period 5 3 Lysing kit use by dates Lysing kits provided by Bertin Technologies must be used before the use by date indicated at www precellys com Use of lysing kits beyond their use by date may result in sudden damage to
10. and Left Right buttons Protocol Create Create a new protocol that will then be displayed in the management sliding program bar see Choose a pre set protocol Edit Modify a pre set protocol Delete Delete a pre set protocol see Choose a pre set protocol Move Change the position of programs in the sliding program bar see Choose a pre set protocol NB the position of the Work program cannot be changed Data Save all programs Transfer all protocols recorded in the Precellys Evolution management to USB unit to an external data storage source USB Load all programs Transfer all protocols from an external data storage source from USB to the Precellys Evolution unit VVARNING this action vvill delete all protocols currently registered in the unit Save file history to USB Back up the equipment file history protocols launched alerts date changes Update equipment from USB Upload a new version of the MMI software Page11 bertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL DESCRIPTION 2 5 Programming Precellys Evolution has been designed to operate at a maximum speed of 10 000 rpm rotations per minute A Precellys Evolution lysing protocol is composed of several successive cycles between which the lid must not be opened Speed and other parameters can be adjusted via the control panel Protocol parameters Values Tube capacities 2 mL 7 mL o
11. echnologies after sales team reserves the right to refuse to perform repairs 9 2 Disposal The cleaning and contamination procedure must be performed prior to disposing of the equipment so as to ensure total human and environmental protection The equipment its spare parts and consumables are the object of separate waste collection procedures in accordance with the EU directive pertaining to waste electrical and electronic equipment WEEE 2002 96 EC Bertin Technologies is under no circumstances whatsoever legally bound to take back this equipment 10 1 adad or ence HEW GENERATION homogenizer SAMPLE PREPARATION amp CELL LYSIS Precellys The Science of Lysing Equipment for Life Sciences return form Gefen CNET TE rhe a Ta eee ee ei naui eee her eee oe aed cum bee weasel sn Pul Elin secre wit thee om ui peer kich mi T bz r ell ee an arp ieee eee mur n ihe mini mun ee ee my epee ES by cel hr p in a nd Ey b www precellys com Phone 33 0 139 306 160 2 A info precellys com Fax 33 0 139 306 185 ber AF1 VIA HOME FECI EZ Page25 Dbertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL Notes bertin Page 26 TECHNOLOGIES Page27 bertin TECHNOLOGIES recellys The Science of Lysing VVVVVV precellys com M
12. etween 2 cycles 1 120 seconds User interface Keypad 9 push buttons Display features 1 LCD colour screen 16 bit 3 5 1 indicator light green and red Capacity Number of tubes 24 12 6 Single tube volume 05mL 2mL 7mL 15 mL z Precellys Evolution is B equipped withremovable tube holders that offer greater flexibility interms of handling different tube types The full list of sample tubes that can be used with Precellys Evolution is available at Pagei3 bertin TECHNOLOGIES PRECELLYS Evolution TRANSPORT AND STORAGE f wm B Avoid violent shocks that may damage the equipment M A Do not connect the unit to the power supply until the installation process is complete Never set the unit at an angle Precellys Evolution must alvvays be used vvhen evenly balanced on its 4 feet Failure to comply with this instruction may result in damage to the internal components or in breakage of the 177 casing To remove the equipment from box do not lift it by holding onto the lid Use either the specially designed extraction handles or the notches located on both sides of the lovver casing of the unit For greater convenience notches are also present in the protective 77 bertin TECHNOLOGIES Page 14 m B A Mi vaene B USER MANUAL 3 Transport and storage 3 1 Transport
13. g tubes Lysing tubes containing samples are positioned in the tube holder and then held in place by the indented plate which must be removed for the loading and unloading of the tube holder The tube holder is removable and is designed in such a way as to allow it to stand on its legs even when fully loaded with tubes It is also possible to operate the equipment when the tube holder is only partially loaded A vacuum system holds the indented plate onto the metallic support ring The prongs of the indented plate retain the tubes in place while the unit is running and the tubes are released when the vacuum pressure is released a few seconds after the end of a lysing cycle INSTRUCTIONS holder 2 7 ou 15mL 5 2 4 Closing the lid Lovver the lid until the locking handle clicks into place 5 2 5 Lysing protocol procedure After confirming the launch of a lysing protocol by pressing the Start button the system creates a vacuum below the indented plate Once the tubes are correctly held in place i e sufficient vacuum the protocol will begin and the green light will flash A timer counts down the remaining time in minutes and seconds until the end of the protocol Example of 2mL indented plate and 2 mL tube holder 3 Put the indented plate back in place NB Time and speed settings cannot be changed while a protocol is in progress P
14. he biological samples and no cross contamination Efficient and homogeneous lysis in all tubes whatever their capacity Page bertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL DESCRIPTION Precellys Evolution in pictures e N 1 Lid Indented plate 2 Locking handle Tube holder 3 Programming and control panel Metallic support ring Containment seal qe 8 Air intake vent 9 USB socket 10 Maintenance sockets 11 On Off button 12 Power supply socket 13 Fuse bertin Page TECHNOLOGIES DESCRIPTION 2 2 Tube motion Thanks to the equipments design and symmetry every tube of the same size strictly follows the same motion to ensure the same level of lysis and homogenization for each sample The centre of gravity of the tubes follows a tri dimensional path on a sphere s surface While the mixture contained in the tubes moves in all directions it moves primarily in the vertical axis to allow for efficient homogenization The movement generated by Precellys Evolution is known as precession movement i e tubes are not rotated The tubes must be compatible with the holder and must be capable of withstanding 600 Gof linear acceleration during 5 minutes without suffering any deformation see usage recommendations at 5 1 1 1 The precession movement during biological sample homogenization causes the temperature of the samples and the equipment to
15. lution must alvvays be used when evenly balanced on its 4 feet Failure to comply with this instruction may result in damage to the internal components or in 77 of the plastic casing S Page21 bertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL MAINTENANCE 7 2 1 Replacing the toric joint The toric joint located on the metallic support ring can show signs of wear over time It is strongly recommended to replace the toric joint whenever the Pressure Alarm message is displayed permanently As an additional precaution the joint should be changed at least every six months 7 2 2 Replacing the containment seal The containment seal should be replaced at least once a year This procedure does not require the dismantling of the metallic support ring 1 Place the interior part of the containment seal against the metallic ring 2 Place the exterior part of the containment seal against the casing bertin Page 22 TECHNOLOGIES MAINTENANCE CLEANING 7 2 3 Replacing the fuse A flat blade screwdriver is required for changing the fuse 8 Cleaning amp Decontamination Flat blade screwdriver For safety purposes and to prevent any damage to the equipment the recommendations listed below should be strictly complied with Do not spray water or cleaning products directly onto the unit especially the air vents and fan intake Alvvays disconnect the equipmen
16. nnect Precellys Evolution to the mains power supply using a This equipment must be powered compatible power cable from a mains supply equipped with a protective earth ground terminal M a Page is bertin TECHNOLOGIES PRECELLYS Evolution INSTRUCTIONS ARNING Gloves should be vvorn and all necessary precautions taken vvith regard to infection related risks when using this equipment and when handling samples i ARNING Never open the lid vvhile the metallic support ring is in motion If the lid is opened vvhile a ysing protocol is in progress the protocol vvill stop automatically bertin TECHNOLOGIES Page 16 s zi USER MANUAL 5 Instructions for use 5 1 Preparing samples Samples must be prepared in Precellys Lysing kits recommended by Bertin Technologies a list of which can be found at www precellys com see 8 1 1 1 5 2 Launching a lysing protocol 5 2 1 Switching on the equipment Once connected to the power supply the equipment is switched on using the On Off switch on the rear close to the AC power input A few seconds after being switched on the main menu will appear on the control panel screen The operator must then select the desired lysing protocol from the Start menu 5 2 2 Opening the lid To open the lid release the locking handle and raise the lid until it will go no further 5 2 3 Loading lysin
17. of the local power supply Ensure that the cable connections are correctly vvired 1 1 4 Fuse The equipment contains one replaceable external fuse located on the rear panel If the fuse needs to be replaced please use the follovving fuse 5x20 T 10 A 250 V The fuse can easily be replaced by the user with the aid of a flat blade screwdriver see 7 2 3 1 1 5 Biological risks Gloves must always be worn when handling samples and all safety instructions related to bio hazardous agents complied with in order to prevent any risk of contamination A sticker bearing the Biological risk symbol is supplied with the equipment and must be placed on the front side of the unit when used with samples of a potentially infectious nature Wiring colour codes are as follows International US code code AMG Brown Black phase Neutral Blue White Earth Yellow amp enc Ground Green ARNING VVaste produced by the normal operation of this equipment must be placed in biological vvaste containers and removed and disposed of by specialized companies P d Pages bertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL INTRODUCTION AZ 1 1 6 Safety warning symbol AWARNING The symbol illustrated below may be found on different parts of the unit Users must comply with all instructions associated with this symbol This equipment may only be repaired by persons auth
18. ore than 1000 scientific protocols and data on the application center Join the Precellys community now peglab a VWR company COPYRIGHTO BERTIN TECHNOLOGIES Tel 33 0 1 39 30 61 60 Design graphique www idsg fr
19. orized to do so by the manufacturer or the distributor 1 1 7 Noise level A 1 7 U 7V 777 VVhen operating at maximum speed i e 10 000 rpm the noise level of the E apparatus does not exceed 70 dBa 1 1 8 Electromagnetic interference WARNING This is a Class A apparatus This equipment may therefore cause radio electric interference in a residential environment In such cases users should take appropriate measures 1 1 9 Intensive use Ifthis device is used in an intensive manner high speed long cycle duration fully loaded with 24 tubes high number of cycles the temperature of the system will increase The increased temperature level may trigger the thermal security protection system In this case the electrical power input of the motor will cut off automatically to prevent the equipment from overheating 1 2 Warranty This warranty is limited to a period of two 2 years and does not Bertin Technologies hereby apply to the following parts fuses toric joints containment seals certifies that this product is indented plate and tube holder free of defects at the time of This warranty is valid from the date of the installed equipment s shipment online registration via the vvvvvv precellys com site This vvarranty is not applicable in the follovving circumstances The equipment has not been installed operated or maintained in compliance vvith the inst
20. r 15 mL Speed from 4 500 to 6 800 rpm and from 7 200 to 10 000 rpm in increments of 100 rom Number of cycles from 1 to 10 Cycle duration from 10 to 900 seconds in increments of 1 second Waiting time between 2 cycles from 1 to 120 seconds in increments of 1 second Programming of speeds between 6900rpm and 7100rpm is not permitted in order to ensure good working order and maximum product life The operator is responsible for programming the unit For each speed range the manufacturer recommends an operating range to prevent abnormal temperature rise see paragraphs 1 1 1 and 5 3 for further details For high speed operation users are advised to reduce the duration of each cycle and to allow the unit to cool down between 2 cycles for at least 2 minutes A security device disables the unit to prevent overheating bertin Page 12 TECHNOLOGIES DESCRIPTION 2 6 Equipment specifications Technical specifications Power supply 230 V 50 Hz EU standard 110 V 60 Hz US standard Consumption 1 kVA Fuse 5x20 T10A H250V Safety information Class A apparatus Physical specifications Width 380 mm Length 520 mm Height 400 mm 635mm with lid open Weight 27 2 kg Operating temperature 15 30 Humidity 15 85 96 RH Altitude 2000m Operating specifications Speed range 4500 10 000 rpm Number of cycles 1 10 Cycle duration 10 900 seconds Waiting time b
21. ructions described in this user manual The equipment has been repaired or modified by unauthorized personnel The equipment serial number has been damaged or removed bertin Page6 TECHNOLOGIES DESCRIPTION 1 3 Equipment references Serial number PRECELLYS EQ02520 200 RD000 0 1 4 Manufacturer s address BERTIN TECHNOLOGIES Parc d activit s du Pas du Lac 10 bis avenue Amp re BP 284 78053 Saint Quentin en Yvelines Cedex France Tel 33 0 1 39 30 61 60 Mail info precellys com 1 5 Technical support In the event that you are unable to find a solution to any technical issues despite the information contained in this manual please contact your nearest distributor 2 Description of PRECELLYS Evolution 2 1 Product overview Precellys Evolution has been designed for lysing and homogenising biological samples contained in glass tubes at variable speeds for the extraction of proteins nucleic acids drugs etc The equipment can simultaneously agitate at high speed tubes containing several millilitres of sample full range of tube sizes available at www precellys com Precellys Evolution offers the following key advantages Easy tube loading an innovative system automatically blocks the lysing tubes during operation Easy decontamination zones which have to be decontaminated are easily accessible Flexible and easy cycle programming cycle speed No alteration of t
22. t from the mains before cleaning Do not use caustic soda or acetone Do not use an aerial decontamination process 8 1 Recommendations The exterior casing of the unit can be cleaned with a sponge or a damp cloth moistened with water or alcohol f 5 i ARNING witch off the equipment and before performing this Page23 bertin TECHNOLOGIES PRECELLYS Evolution USER MANUAL CLEANING DECONTAMINATION 8 2 Example decontamination procedure In the event that a tube breaks during a lysing cycle decontaminate parts that may have been contaminated with an appropriate disinfectant The choice of decontamination procedure is the sole responsibility of the user Parts that may have been contaminated can be cleaned with a sponge or a damp cloth moistened with bleach diluted at 6 Cl If another decontamination procedure is to be used please first contact our technical support team to ensure that the chosen procedure is compatible with the equipment 9 Repair and Disposal 9 1 Repair The equipment must be cleaned and decontaminated in accordance with the procedure described in 8 2 before shipment for repair The equipment must be sent with a return form see annex 1 indicating clearly at the very least the procedure used the persons involved and the date on which cleaning and or decontamination was performed If the return form is missing from the consignment Bertin T
23. tubes ALARM 6 Alarm messages VVARNING Never open the lid vvhile the metallic support ring is in motion Alarm message Possible cause Action Pressure error Tubes recommended by Bertin Technologies are not correctly positioned in the holder 1 Reposition the lysing tubes in the holder 2 Press on the centre of the indented plate during the vacuum phase lid open The indented plate or tube holder are not positioned correctly Reposition the indented plate on the metallic support ring and ensure that it is positioned correctly using the positioning guide The toric joint is not positioned correctly Reposition the toric joint on the metallic support ring and ensure that it is correctly adjusted to fit on the metallic support ring The indented plate is damaged Replace the indented plate The toric joint is damaged The vacuum circuit is not working correctly Replace the toric joint see 7 2 1 1 Switch off the unit 2 Contact Bertin Technologies technical support team Lid error Motor error The lid is not closed properly 1 Check that nothing is preventing the lid from closing 2 Press the lid and ensure that the handle is locked properly The detection system is faulty The motor temperature has reached its maximum safety limit 1 Switch off the unit 2 Contact Bertin Technologies technical support team 1 Leave the unit s
24. witched on to allow the ventilation system to keep running 2 Ensure that the air intake and extraction vents are unobstructed 3 If after 30 continuous ventilation the alarm message is still displayed contact Bertin Technologies technical support team The power supply is not compatible with the equipment Check the coherence of the voltage specified on the rear of the equipment and the voltage delivered by your local electric power supply The speed regulation or detection system is faulty 1 Switch off the unit 2 Contact Bertin Technologies technical support team bertin TECHNOLOGIES Page 19 USER MANUAL PRECELLYS Evolution MAINTENANCE 7 Maintenance VARNING Prior to any maintenance operation the equipment must be cleaned and decontaminated according to the infection risk associated vvith the samples 7 1 Troubleshooting guide The list of the main issues encountered during normal operation and the actions to take are listed in the table below being treated and using suitable personal protection equipment M ssue encountered Possible cause Action The fan is not vvorking No power supply to 1 Check the mains voltage mains plug 2 Ensure that the voltage of the unit matches that delivered by the mains povver supply 3 Ensure that the unit is plugged in properly Faulty fuse Replace the fuse see 7 2 3
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