Hardware User`s Manual Hot plate
Contents
1. If the temperature is below 20 0 C or above 70 C the display will not show the temperature but will show If the internal temperature probe does not work the display will show in this case contact the technical service The LE7406 display shows instead of a numeric value If the internal triac is shorted the Hot Plate has a protective thermal switch that opens the circuit when the The Hot Plate heats always temperature reaches 7o C This can be seen when the equipment continues warming despite the HEAT LED is off Hot plate 17 Panlab Md 11 PREVENTIVE MAINTENANCE EXPERIMENT HOT PLATE CLEANING TRANSPARENT CYLINDER CLEANING CHECK TRANSPARENT CYLINDER PLACING Hot plate 19 12 SPECIFICATIONS Panlab H POWER SUPPLY Input voltage 115 230V Frequency 50 60 Hz Fuse 2 fuses 5mm 20mm 2 250V Fast Maximum power 190W Conducted noise EN55022 CISPR22 CISPR16 class B Equipment start up time 10 min WARMING SPECIFICATIONS Temperature range 459 62 C Display resolution 0 19C Accuracy 0 3 9C Highest temperature 70 9C without probe feedback Security switch 709 TEMPERATURE PROBE Technology Measurement range showed Linearity Accuracy Active probe 209 709 0 1 0 1 C ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non conden2. el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales a otras formas de reciclaje de uo usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente 5 Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique 5 Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te durfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zuruckzugeb
3. listed in the following table LE7406 RS 232 PC Com Port RS 232 cable LE7406 Pedal 6 35mm jack Hot plate 12 Panlab Harvard 8 2 START UP When the instrument is switched on switch to ON the screen displays the present temperature of the hot plate between 20 C and 70 C If the temperature is above below this the screen will display When the button is pressed the required temperature appears on screen with a flashing decimal dot Press the A ARROWS to modify increase or reduce the required SELECT temperature Press the button again to select the required temperature which will be stored in the device s memory eliminating the need to adjust it every day While the required temperature is being modified the plate heating system remains HEAT active at the previously set temperature The LED remains on while the heating system is active The amount of electrical power applied to the plate is proportional to the difference between the actual and the target plate temperature Once the HEAT required temperature is reached the led 9 heating will go off and the plate will stop When the plate temperature falls below the required temperature due to the heat released into the environment the heating system switches on automatically until the required temperature is reached again WARNING the stability of the plate s temperature does not only depend on the heating control s
4. E Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Hot plate Harvar Panlab Harvard 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially designed to p
5. Hardware User s Manual Hot plate Hot plate References LE7406 76 0113 Version V29 10 2014 Panlab s l u C Energ a 112 08940 Cornella de Ll Barcelona Spain www panlab com International Calls 34 934 750 697 Domestic Call 934 190 709 Fax 34 934 750 699 Info panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be A exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C Warning about metal surface that supply electrical shock when it s A touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEEE A 2 GOOD LABORATORY PRACTIC
6. PARATUS Figure 7 Front Panel e HEAT LED that comes on while the unit is in the process of reaching the temperature selected e C This screen indicates the present plate temperature When the SELECT button is pressed the required temperature is displayed e SELECT Press this button to set the required temperature using the ARROWS The Up arrow increases the setting temperature and the Down arrow lowers it The temperature can be set from 45 C to 62 C in increments of 0 1 C e ARROWS These buttons are used to select the target temperature once the SELECT button has been pressed with the decimal dot flashing To validate the selection press the SELECT button again the decimal dot will not flash and the display will show the present plate temperature e TIMER The screen indicates the time elapsed from the moment the pedal is pressed start until it is pressed again stop Hot plate 10 Panlab 7 2 REAR PANEL PEDAL RS 232 POWER Figure 8 Rear Panel e PEDAL 6 35mm female jack The foot switch is connected to this connector e RS 232 DBg female connector to connect the control unit to the computer serial port e POWER Power inlet main switch and fuse holder Hot plate 11 Panlab H 8 OPERATINGTHE LE 7406 8 1 EQUIPMENT CONNECTION This illustration features a sample system connection Figure 9 Equipment connection The connections and necessary cables are
7. e Cornell de Llobregat Spain 26 06 2014 Hot plate 20 A Harvard Apparatus Panlab H GB Note on environmental protection GB After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en
8. en Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altgeraten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt P Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente IX Nota Ambiental Ap s implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a col
9. finally dry it with a dry cloth WARNING Before cleaning the Hot Plate unplug it from mains 8 3 2 CLEANING THE TRANSPARENT CYLINDER WARNING To clean the transparent cylinder never use alcohol or alcohol derived products otherwise streaks will appear in clear plastic To clean the transparent cylinder you can use a slightly dampen cloth and then dry it with a dry cloth If it is too dirty the cloth can be dampen with a soap solution then remove the foam with a damp cloth and finally dry it with a dry cloth Hot plate 15 Harvar Panlab Hever 9 WORKING WITH THE SEDACOM SOFTWARE The purchase of the Sedacom software is needed for transferring the data to a computer please contact your local sales delegate for more information The Sedacom software reference is composed by a USB Flash key containing the software Installer a License for use and Sedacom User s Manual Follow next instructions e Please refer to the Sedacom User s Manual for instructions on how to install and use the software with the present device e A serial port RS232 communication cable provided with the present device is needed for the connection of the present device to the computer in which the Sedacom software is installed Please refer to the present User s Manual chapter 8 1 for instructions on how to connect this cable to the device e f the computer does not have any serial port the RS232 USB adapter is needed ref CONRS232USB contact y
10. ocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribui o para a protec o do ambiente 5 Hot plate 21
11. of the equipment it is essential that the attached PC itself conforms to basic safety and standards and is set up accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Class A equipment is intended for use in an industrial environment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference N when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if WARNING not installed and used in accordance with these instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Hot plate Panlab 4 MAINTENANCE WARNING Failure
12. osition CORRECT INCORRECT Figure 4 Fuses position 5 Insert the fuse holder again positioning it according to the voltage in the AC net 115V POSITON 230V POSITION Figure 5 Fuse holder position 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety reasons never open the equipment The power WARNING SUpply has dangerous voltage levels Hot plate Panlab 5 TABLE OF CONTENTS 1 SYMBOLS TABLE 2 GOOD LABORATORY PRACTICE 3 UNPACKING AND EQUIPMENT INSTALATION 4 MAINTENANCE 5 TABLE OF CONTENTS 6 INTRODUCTION 6 1 ACCESSORIES SUPPLIED 7 EQUIPMENT DESCRIPTION 7 1 FRONT PANEL 7 2 REAR PANEL 8 OPERATING THE LE 7406 8 1 EQUIPMENT CONNECTION 8 2 START UP 8 2 1 SETTING TO THE REQUIRED TEMPERATURE 8 2 2 TESTS WITH ANIMALS 8 3 EQUIPMENT CLEANING 8 3 1 CLEANING THE HOT PLATE 8 3 2 CLEANING THE TRANSPARENT CYLINDER 9 WORKING WITH THE SEDACOM SOFTWARE 9 1 TRANSMISSION PARAMETERS 10 TROUBLESHOOTING 11 PREVENTIVE MAINTENANCE 12 SPECIFICATIONS Hot plate Harvard Apparatus 10 10 11 12 12 13 14 15 15 15 15 16 16 17 18 19 Panlab H jede 6 INTRODUCTION The LE 7406 is a device used to assess a rodent s response to pain induced by heating the rodent s legs The nociceptive stimulus comes from a metallic surface which is electrically heated in its entirety The animal is placed on the metallic
13. our local sales delegate for more information WARNING the RS232 communication cable provided with the device is used for any connection of the device with associated software Sedacom etc Even when the device is used without software in first instance this cable is to be preserved and kept in a secure place in case the need of using the system with a software arises in the future In this last case if the user lost the cable new one should be purchased to his local sales delegate ref CONRS232 The warranty duration of this cable is the same than the warranty duration of the device 9 1 TRANSMISSION PARAMETERS The transmission parameters are as follows 9600 bauds Databts 8 Control signals CTS and RTS Hot plate 16 Panlab 10 TROUBLESHOOTING This table features instructions solve the most frequent problems PROBLEM SOLUTION Ensure that the voltage of mains is the 122222 same as that selected the fuse holder Check the condition of the fuses Check that the cable is connected between the RS 232 device and the PC serial port see Figure 9 Make sure that both the serial port as the device selected in the Sedacom program The equipment does not send data are correct to the program Sedacom The equipment will send data only when you press the pedal and the stopwatch is running then the time is stopped and is necessary to press the pedal again to start a new measurement
14. pements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2013 Class A FCC FCC47CFR 15B Class Safety of machinery EN ISO 12100 2010 This equipment complies with the limits for class A equipment in accordance with CISPR 11 definition and is classed as a Class A digital device pursuant to CFR Title 47 part 15 of the FCC Rules and is intended to be used in an industrial environment En consecuencia este producto puede incorporar el marcado CE y FCC Consequently this product can incorporate the CE and FCC marking C En cons quence ce produit peut incorporer le marquage CE et FCC En representaci n del fabricante Manufacturer s representative Carme Canalis A O A a En repr sentation du fabricant General Manager WE Panlab s l u a division of Harvard BioScienc
15. rotect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits A For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Hot plate Panlab Hever PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity
16. sing 09 to 50 C non condensing COMUNICATIONS OUTPUT Standard interface RS232C Connector Delta 9 contacts female connector TIMER Range 0 005 to 999 995 Resolution 0 015 DIMENSIONS Width x Height x Depth 232 mm 124 mm 297 mm Weight 5 31 kg Hot plate 19 Panlab H DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto HOT PLATE Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 7406 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d qui
17. surface and the latency of the motor response induced by the pain is measured m Figure 6 LE7406 Hot Plate The stimulation surface can be heated to a temperature between 459C and 629C which is user selectable and which the device will maintain constant 0 1 C throughout the test The user starts a timer by pressing a PEDAL when the animal is placed on the metal surface Following a period of latency the animal licks its leg This reaction is the usual evaluation criterion for the test The user presses the PEDAL again to cut off the time once the animal s response is visually detected The response time determined with a precision of 0 01 sec is displayed on an LCD screen until the next test The information can be transmitted to a serial printer or a PC via an RS 232 communications port and the SEDACOM software not included should be purchased separately WARNING The temperature of the Hot Plate hot plate is adjustable between 45 and 62 In case of broke down a resettable thermal protection system prevents the temperature exceeds 7o C Take caution when touching the hot surface Hot plate Panlab 6 1 ACCESSORIES SUPPLIED e LE7406 HOT PLATE 1 PEDAL 1 e Power supply cable 1 RS 232connection cable 1 Hot plate Panlab H Ade 7 EQUIPMENT DESCRIPTION 7 1 FRONT PANEL SELECT UP DOWN ARROWS HEAT PLATE TIMER Y Panlab HARVARD HOT PLATE LE 7406 AP
18. temperature can be modified Press the UP DOWN ARROWS to modify the required temperature Y Once the new required temperature has been reached press the SELECT button again 50 This value will be stored permanently in the unit s memory will prevail throughout the work session There is no need to input the required temperature every day as the value is stored permanently in memory The led HEAT will remain on while the equipment is heating HEAT Following 5 minutes of pre heating the unit is ready for use Hot plate 14 Panlab H 8 2 2 TESTS WITH ANIMALS 1 Place the experimental animal on the plate TIMER pressing the PEDAL once at the same time 2 As soon as the animal responds by licking its leg press the PEDAL once to stop the timer 3 screen displays the latency of the response which will remain until the next test 4 If the device is not connected to the RS 232 port write down the latency value Otherwise the peripheral device serial printer or PC will record the value displayed on screen 5 Remove the animal from the plate and go back to step 1to repeat the procedure 8 3 EQUIPMENT CLEANING 8 3 1 CLEANING THE HOT PLATE To clean the hot plate you can use a cloth dampen with water and then dry it with a dry cloth In case that there would be traces of faeces and urine you can impregnate the cloth with a soapy solution then remove the soap residues with a dampen cloth and
19. to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT OR VOLTAGE SETTING CHANGE In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment or if the equipment voltage setting is incorrect check fuses according to the following procedure 1 Remove power cord from the power inlet Hot plate A Harvard Apparatus Panlab 2 Open fuse holder by pulling the flange with a regular screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver e E Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct p
20. ystem but also on environmental conditions Stability is lower if the unit is in a place where ambient temperature is variable near an open window air conditioning unit etc or if there is a substantial difference between the required temperature and the ambient temperature Following a brief pre heating period between 5 and 10 minutes depending on the difference between required and present plate temperature the required temperature should be reached The unit is now ready to begin testing with animals The test consists of visually detecting a characteristic motor response in mice when they are placed on a hot plate that is following a very brief period of exploration the mouse licks its leg The user measures the time elapsed from when the animal is placed on the plate start point the timer is started by pressing the PEDAL once until it licks its leg end point the timer is stopped by pressing the PEDAL again Hot plate 13 Panlab A The latency of the response is shown on the screen to a precision of 0 01 seconds The latency time remains on screen until the pedal is pressed again to run another test The procedure to be followed is described as follows 8 2 1 SETTING TO THE REQUIRED TEMPERATURE Switch on the Hot Plate Press the SELECT button once The present gt required temperature will be displayed SELECT screen The decimal dot flashes indicating that the value of the required
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