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Grapevine User Manual v08

1. Figure 1 13 Front panel view of the Grapevine Scout left side SE in digital out SR 3 i Lu C digital parallel n J TIT SS A V7 e d N aJI j ES 4 N CEES ree HEET N N US CE TON Ve f al M TRANE ER RER ag WC SET DEES Sale Figure 1 14 Front panel view of the Grapevine Scout right side Back Panel The back panel of the Scout provides ports for interfacing the processor with computers over an Ethernet network expansion ports and auxiliary control interfaces as well as the device serial number For discussion on the expansion and auxiliary ports please refer to the Grapevine NIP section Note The VGA plate on the back of the Scout processor should remain attached at all times The VGA port beneath is for Ripple development and testing purposes only expansion nv auxiliary 7 ethernet ripple ge salt lake city utah usa SN R01381 0001 Figure 1 15 Back panel view of the Grapevine Scout Ei Document Version RO1550_08 Chapter 1 Hardware Overview om Se Chapter 2 Installation and Setup This chapter describes various options for connecting the Grapevine NIP to one or more computers for visualizing and recording data Connecting the Hardware The Grapevine NIP must be connected via a Gigabit Ethernet connection to computers running the Trel
2. Figure 2 1 Connecting a Grapevine NIP to a computer with an Ethernet cable Grapevine User Manual Document Version R01550_08 ERS Windows 7 Network Configuration Configuring the Grapevine NIP to work with a Windows 7 computer can be done completely through the Windows 7 Control Panel The two main processes include configuring the Grapevine s Network Interface Card to the proper IP address and configuring the firewall Configuring the Network Interface Card connected to the Grapevine NIP Step 1 Open Control Panel Click on the Windows button and then Control Panel to open the Windows 7 Control Panel Note The following steps m this manual are written when viewing the control panel by large icons Ripple Documents Pictures Music Computer Control Panel Devices and Printers Default Programs Help and Support All Programs Shut down View by Category Y Category User Accounts and Fam W Add or remove user accoun W Set up parental controls for Large icons Small icons Appearance and Personalization Change the theme Change desktop background Figure 2 3 Step 1 Note View the Control Panel by Large icons Document Version RO1550_08 Chapter 2 Installation and Setup Bi Step 2 Open Network and Sharing Center Click on the Network and Sharing Center icon to access the network settings Step 3 Open Network Interface Card List Click on
3. Figure 3 3 Trellis Instruments Instrument Network CN po Pe xs File Tools Help amp trellis Oo a 8 Instrument Network Network Status Active 1 Instrument Online 4 Grapevine NIP 1 ENEE a MH Front Ends BEES aha H A 1 nano2 RO0894 0004 v3 2 Network Address 192 168 H 42 210 U 8 1 stim RO1289 0005 v2 1 Network Traffic WU c 1 micro RO0249 0112 v5 2 carrie pata Rate Mbps I N C 2 EMG R02008 0001 v2 1 E EECH U D 1 digital IO R00591 0027 v3 1 Data Rate Mb s 30 05 N D 2 analog IO R00407 0021 v3 2 a Data Streams Config Packets Received 4 743 4 V Analog 1k Config Rate Mb s 0 00 7 D 2 analog 10 UDP Packets Received 366 610 4 V Analog 30k v D 2 analog 10 4 V Digital Events E EE eem v D 1 digital IO a Hl Hi Res Data Rate Mb s 0 00 v A l nano2 Config Packets Sent 1 v B 1 stim v CL micro SSES oie d G2 EMG UDP Packets Sent 1 a d LFP v A 1 nano2 v B 1 stim v CL micro 4 V Raw Signal d A 1 nano2 v B 1 stim v CL micro s V Spike v A 1 nano2 Advanced Options v B 1 stim gt V CL micro IP4 Network See dl oie 4 7 Stim Use localhost Start Avg 0 0 ms Max 0 0 ms gt V B 1 stim Grapevine User Manual Document Version RO1550_08 28 iS IS 8 8 Ss Ss Ss SS Figure 3 4 Trellis Instruments Front End User Controlled Settings BEE HB g Z Ocococ SE e z lt Jo BEBE SB ae B
4. Configuring Spike Sorting Parameters Trellis possesses the ability to sort spikes online via a time amplitude method commonly referred to as the hoop method Trellis supports the definition of up to four hoops per unit and up to four units per channel Spike Scope provides a graphical interface for defining and editing hoop unit parameters To turn this interface on check the Enable option in the Sorting control This will bring up a set of four unit editing controls in the top right of the spike window pink green yellow and blue as seen in the left panel of Figure 3 12 below To define the first hoop for a given unit click on the colored circle corresponding to that unit as shown in the right panel of Figure 3 12 below Sorting Spike Persistence Sorting Spike Persistence ral Enable 50 Ral Enable 50 Clear Units Clear Spikes Clear Units Clear Spikes Figure 3 12 Trellis Applications Spike Scope sorting controls ES The unit editing control will expand to reveal three circles and the cursor will change to a shape indicating hoop drawing mode as shown in the left panel of Figure 3 13 To define the hoop click on the display where the hoop should start and while holding the mouse button down drag it to the point where the hoop should end This will cause all spikes that pass through the hoop to change to the chosen color as shown in the right panel of Figure 3 13 To create another hoop for the same unit click on
5. ES e Selected Signals This panel enables the selection of individual channels and data streams to be recorded o Raw Signal includes a continuous waveform data stream containing the entire bandwidth of the FE amplifier The raw data is sampled at 30 kS s per channel This data is saved in the ns5 file format o Hi Res contains a continuous waveform data stream with 24 bit floating point precision which allows for better precision at the low end of the spectrum as well as a greater dynamic range which can be useful for ECoG and EMG data The Hi Res signals are sampled at 2 kS s and are digitally filtered using a low pass IIR filter of 500 Hz default with 4 order Butterworth characteristics This data is saved in the nf3 file format o LFP includes a continuous waveform data stream for low frequency waveforms such as LFP ECoG EEG and EMG The LFP data stream is sampled at 1 kS s per channel and digitally filtered using a low pass IIR filter of 250 Hz default with 4 order Butterworth characteristics This data is saved in the ns2 file format o Spike data includes timestamped neural events that cross the user defined threshold The spike data is sampled at 30 kS s per channel and is digitally filtered with a high pass filter of 250 Hz default The Spike data is stored in a 1 7 ms 52 sample data segment in the nev file format Note The Timestamp associated with each spike event is the beginning of the 52 sample spike window 15
6. Start Recording Button e Press this button to begin recording Pause Recording Button jj Press this button to pause recording Press it again to resume recording Stop Recording Button gg Press this button to stop file recording and save the data file Enable Remote Control When this option is enabled other PCs connected to the instrument network can via UDP instruct File Save to start pause and stop recording Contact Ripple technical support for more information about controlling Trellis via UDP om Se Appendix A Technical Information This appendix describes in detail the technical aspects of the Grapevine system This appendix is not intended for use as a service or repair manual Modification of any Grapevine components without the written permission of Ripple will void the warranty The Grapevine system consists of components that are designed to acquire hundreds of channels of biopotentials and other experimental data The Grapevine NIP PN R00244 is a highly specialized stand alone computer system equipped with a 1 86 GHz Intel Core2Duo SL9400 Processor running a custom Linux kernel along with 4 independent medically isolated ports for connection to Front Ends FEs Up to 16 FEs can be connected to a NIP and each type of FE is described in the following sections All Grapevine FEs are powered by the Grapevine NIP through the front end ports All of the acquired signals are filtered amplified and sampled digit
7. The maximum allowable time for a pattern is hr If the duration of the stimulation train is not an integer multiple of the stimulation period 1 frequency a warning will be displayed next to the Frequency parameter as shown in Figure 3 23 below and the train will have an empty partial period at the end of the pattern noted by a red box in the Train Display The warning text can be refreshed by clicking on the warning symbol P continuous stimulation will occur continuously at a selected frequency until the stop button is pressed Duration once Lei burst 1500 msec continuous The train duration is not an even multiple of period on electrodes elec A 1 01 Figure 3 23 Trellis Applications Stimulation Controller Burst Duration Warning Waveform elec B 1 14 Period 100 00 msec 10 00 Hz Train 1000 msec 10 pulses 1600 msec Figure 3 24 Trellis Applications Stimulation Controller Waveform Period and Train Display Waveform Display Shown in the top center of Figure 3 21 and in Figure 3 24 the Waveform display shows one cycle of the stimulation parameters for Phase 1 the IP Interval and Phase 2 The time and amplitude scales of the Waveform display can be altered by positioning the cursor over the Waveform display and using the buttons that appear or by holding down the Shift key Alt for amplitude and then scrolling the mouse wheel Period Display Shown below the Wavef
8. Change Adapter Settings on the left side of the Network and Sharing Center panel Step 4 Open the Network Adapter s Properties Identify which of the network cards on the list is used to connect to the Grapevine NIP It may help to rename this network card to Grapevine NIP NIC for clarity in the future as changing the properties on this network card may make the Grapevine NIP no longer function properly Right click on the appropriate Network Adapter and select Rename to rename the network card Step 5 Configure the Network Adapter s Properties Right click on the card again and select Properties from the list Uncheck all of the boxes except for Internet Protocol Version 4 TCP IPv4 Then select Internet Protocol Version 4 TCP IPv4 from the list and click the Properties button on the right side of the pane Change the settings as shown in Figure 2 4 below with the IP address set to 192 168 42 129 subnet mask set to 255 255 255 0 and the gateway blank When these settings are entered click OK to apply them and close this window Also click OK on the Network Interface Card s Properties window to apply the settings and close the window Internet Protocol Version 4 TCP IPv4 Properties General You can get IP settings assigned automatically if your network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings Obtain
9. Trellis Software Suite ES Instruments The Instruments tab contains general information about the instrument network and detailed information about individual NIPs and Front Ends When Trellis is started the Instrument Network will display the Network Settings panel similar to Figure 3 3 This view displays network status a plot of aggregate network load and details on the composition of network traffic If the data rate is too high for the computer to keep up a warning message will be displayed when network data packets are lost All available Front Ends and data streams will be listed under their associated Grapevine NIP in the Instrument Network tree on the left side of the window Some configuration options are detailed below e User controlled parameters for Nano2 style Front Ends can be set by clicking on the Front End entity as shown in Figure 3 4 e Calibration routines for Micro2 Stim and Nano2 Stim can be run and loaded from saved data sets e Specific data streams can be configured for each Front End by expanding the Data Streams tree and individual electrode channels can be initialized for spike or stim data streams where appropriate e Information about a Grapevine system can be accessed by clicking on the corresponding entity in the Instrument Network Selecting the Grapevine NIP 1 item in the Instrument Network brings up an interface to configure the Grapevine NIP as shown in Figure 3 5
10. for clarity and technical accuracy Version 04 e Addition of Test Adapter Instructions Appendix e Addition of front end cable use instructions Version 05 e Updates to Chapter 1 and Appendix A to include new EMG Front End e Updates to Adapter Appendix to include additional adapters e Updates to File Save Application to include file segmenting e Updates to Impedance Analyzer Application to include Nano Front Ends e Updates to Windows and Macintosh configuration recommendations e Updates to Troubleshooting to include instructions for OFS and Windows Configuration e Inclusion of sample data location Version 06 e Updates to Chapter 1 Chapter 3 and Appendix A to include new Nano2 Front End e Updates to Adapter Appendix for new touchproof adapter e Inclusion of new Appendix C Hardware and Software Configuration Version 07 e Updates to Chapter 1 Chapter 3 and Appendix A to include new Nano2 HV and Nano2 Stim Front Ends Version 08 e Updates to Chapter 1 Chapter 3 and Appendix A to include new Micro2 Micro2 HV and Micro2 Stim Front Ends Correction to Digital I O output routing removal of stimulation event markers not currently functional Document Version R0O1550_08 Appendix G Revision History
11. 32 high impedance microelectrodes The Micro HV and Micro2 HV are similar to the Micro and Micro2 and include additional circuitry for high voltage protection in applications where nearby stimulation may occur Surf S The Grapevine Surf S records from up to 32 single ended surface electrodes It has additional ESD protection and is designed for common surface recording electrodes such as EEG applications EMG The Grapevine EMG records from up to 16 differential electrodes It 1s designed for surface and implanted differential signals such as bipolar EMG Note All FEs have been internally tested to meet the standard leakage and isolation requirements The Grapevine Micro FEs Surf S and EMG have additional safety protection ES Front End to NIP Connector Grapevine Micros Surf S and EMG The top panel contains the status indicator and an Omnetics Bi Lobe terminal to connect the FE to a front end cable The status indicator on the Grapevine FE will turn green when a FE is properly connected to the Grapevine NIP and initialized e m D SE y 5 U cosse90 ke K P N Omnetics Bi Lobe to grapevine NIP status indicator Figure 1 6 Top panel of the Grapevine FE Micros Surf S and EMG Table 1 3 Grapevine FE status indicator Micros Surf S and EMG Status Indicator Meaning No connection Either the FE is not properly connected to a front end off cable or the cable is not connected
12. 34 Analog I O shown and Digital I O Front End information 65 Document Version R01550_08 Appendix B System Information om Appendix C Hardware amp Software Configuration This appendix describes the startup states of the neural processors Front Ends FEs and Trellis as well as what information is saved in the Trellis Configuration File Each component of the Grapevine Neural Interface System has a default startup state that is set when the system is powered on These hardware and software states can be changed to user desired configurations by loading a Trellis configuration file Hardware Below are the default startup states that are specific to the neural processors and Front Ends and will be shown as such in the main Trellis program All of the hardware settings below are dependent on which Front Ends are connected to the system These states can be changed in the active system through Trellis Xippmex or user developed C applications All of the below hardware states will be reset to default when the processor is powered off and on e Data Streams o LFP On o Hi Res On o Raw Off o Spike On o Stim On o Analog 1k Off o Analog 30k On o Digital Events On e Global Thresholding o Method Multiple of RMS Median o How to Apply Single Only o Level 5 0 uV o Spike Sorting Disabled e Digital I O Options o Loopback UDP triggered digital output On o Capture digital input on parallel bit c
13. Controller app Figure 3 20 If the wrong value is entered the step size can be changed by altering the value and selecting Apply For Micro2 Stim and Nano2 Stim FEs this amplitude step size is user selected through a drop down menu e Stim Configuration This panel enables the saving of individual electrode stimulation patterns that have been defined as well as the multi channel stimulation score described below This may be useful when using a large number of electrodes and or defining complex stimulation patterns A saved configuration file stm can be loaded or deleted from this panel e Multi Channel Stim The control for expanding and collapsing the Multi Channel Stim display is contained on the left side of the Stimulation Controller window By default this panel is collapsed and can be expanded by clicking the symbol On the right of the Stimulation Controller window are the Stim Parameters and Multi Channel Stim control panels Below is a detailed description of each panel Stim Parameters This panel enables the display of and control over the parameters of the present stimulation pattern for one or more channels electrodes The parameters for any channel s present stimulation pattern can be viewed by clicking on that channel in the hardware list If multiple electrodes are selected the present pattern for each channel is displayed in the Preview section of the Stim Parameters panel The present pattern for multi
14. Figure 2 5 Correct network adapter settings for IPv4 Settings Grapevine User Manual Document Version RO1550_08 26 om Chapter 3 Trellis Software Suite The Trellis software suite consists of a main program Trellis responsible for managing the instrument network and recording data to disk and a set of applications Raster Spike Scope Spike Grid Impedance Analyzer and Stimulation Controller that provide the ability to visualize signals configure spike extraction and sorting measure electrode impedance and control stimulation The Trellis software suite installer for a chosen operating system can be downloaded from the Ripple website Check the Ripple website for software patches and new releases To install the Trellis software suite simply execute the installer and follow the prompts A computer restart is recommended after following the installation process Trellis is supported for Microsoft Windows 7 Mac OSX 10 7 10 9 and Linux tested with Ubuntu 12 04 This section is based on the Trellis 1 5 0 software release Note Updates to the Trellis software suite often require a complimentary software update to the Grapevine NIP Contact Ripple Support before downloading and installing any Trellis software updates Using Trellis Software Before running Trellis ensure that all Front Ends are connected to front end cables all cables are connected to the Grapevine NIP that the NIP is turned on green LED indicator and that
15. The differential version uses a 10 1 voltage divider and includes a switch to set the second SMA terminal to ground The Omnetics to Samtec adapter RO1O50 is required to acquire test signals with Nano style Grapevine Front Ends as shown in Figure D 1 on the right Be sure to mate Front Ends to the adapters so that the pin markers align Grapevine Micro Micro Stim Surf S Grapevine Nano FEs animal use only Mc SX nt Figure D 1 Recording Front End test adapters and cables Stimulation Front End Testing For stimulation Front Ends a 10 kQ test load board RO1913 is included to allow users to test stimulation on all 32 channels with a known impedance load Test points are added to each channel location and ground as shown in the image below which can be useful for viewing the stimulation signal on an oscilloscope The delivered stimulation voltage can also be acquired via Trellis by recording the stimulation data stream Be sure to mate Front Ends to the adapter so that the pin 1 markers align Micro Stim FE in image below is rotated for display purposes 0200009000000 00006660660000066 BESSRERERSERERRRERESEERASERERERE Stim FEM Test Board ohms Load Figure D 2 Stimulation Front End load test adapter 73 Document Version RO1550_08 Appendix E Adapters Appendix F Troubleshooting Guide om Se This appendix provides helpful hints and solutions for troubleshooting the Grapevine syste
16. Turn signal clipping off Increase the time range displayed decrease sweep speed Decrease the time range displayed increase sweep speed Toggle between sweeping and scrolling view Color waveforms round robin by FE input channel Reset colors Select all lines Unselect all lines Move selected line up Move selected line down Set display height for selected lines Decrease display height of selected lines Set display height equal for selected lines Increase display height for selected lines Group selected lines and change display height to fill screen Set voltage scale display for selected lines Decrease voltage scale for selected lines Equalize voltage scale for selected lines Increase voltage scale for selected lines Adjust voltage scale to fit signal range for selected lines EES Grapevine User Manual Document Version RO1550_08 E ERS Spike Grid The Spike Grid application provides a grid view of the spike waveforms for one or more data channels By default all FE channels connected through the Grapevine NIP are displayed Double clicking on a channel display launches the Spike Scope application for that channel see next section for details on the Spike Scope Note If the width of the Heat Map Display is too narrow the electrode labels will not be visible File Tools Help LoadMap Spike Grid Heat Map Scale 200uv We zl Hold 10 Spikes Clear Figure 3 9 Trellis Applications Spi
17. all units can be deleted at once by clicking the Clear Units button in the Sorting section of the Spike Scope tool bar as shown in the right panel of Figure 3 15 The presently defined hoops are not deleted when sorting is disabled A fully sorted set of three unique spike units is shown in Figure 3 16 Sorting Spike Persistence Ed Enable 50 Figure 3 15 Trellis Applications Spike Scope sorting controls deleting hoops E T BD Spike Scope elec A 1 01 Ecje e el Current Signal Signal History Display Range Thresholding Sorting Spike History V Enable 50 ei a s a al 1 Wel an sf Auto wd AS 4200 uv We 3 b lt 2026 gt Clear Units Clear Spikes 180 uy LO OM 120 uv 20 UM 60 UV 30 uv 30 ON 60 UN 30 OM t20 u 150 uv 160 OM Figure 3 16 Trellis Applications Spike Scope with sorting enabled for three unique units Document Version RO1I550_08 Chapter 3 Trellis Software Suite ES Impedance Analyzer The Impedance Analyzer can be used to measure the impedance of recording electrodes attached to Front Ends that support impedance measurement The range of accurate measurement is for electrodes between 10 kQ and 1 MQ for Nano Nano2 Micro and Micro2 and between 10 kQ and 400 kQ for Nano2 Stim Nano2 HV Micro2 Stim and Micro2 HV All supported FEs will be displayed on the left hand side of the window when the application is st
18. and a compliance voltage of 8 5 V The discrete current step size can be configured to be 1 2 5 10 or 20 uA step 75 steps for the highest step size All stimulation is in reference to the ground connection Stimulation and recording can be done simultaneously with the Nano2 Stim FE The stimulation output can be calibrated to ensure the delivered current is within 5 of desired stimulation levels see the Instruments section of Chapter 3 for details on running calibration Note The Grapevine Nano2 Nano2 HV and Nano2 Stim are not intended for clinical or research use with humans Note There is a known issue with the Nano2 Stim FEs where simultaneous stimulation on multiple electrodes causes distortion in the stimulation output in the scenarios below The solution to this is to use a higher stimulation step size For example 1f you want to stimulate on 8 channels simultaneous at a level of 20 uA the recommended step size would be 5 uA step This issue only occurs during simultaneous stimulation across multiple electrodes not during interleaved or off set multiple electrode stimulation and does not occur for the 20 uA step step size Number of Simultaneous Channels Stimulation Step Size Darlene 3 4 5 8 9 or more uA step omnetics adapter electrodes or adapters to grapevine NIP guide pin guide pin 4 guide hole E E guide hole Ref 1 E cno cnD re ch2 chi ch1 I cn cha E cn ch3 IO chn
19. and analog input data may be downloaded from here Ca EH D BEE File Tools Help trellis Selected Signals Ux sig File C Users Ripple Trelis dataFiles 20150803 datafile e 4 V Analog 1k Auto Increment gt V D 2 analog IO Eaa 4 V Analog 30k Fle Settings Format NEV NSX 2 2 Recording Status gt V D 2 analog IO y Digital Events Comment 0 200 characters Clear Elapsed Time 00 00 00 00 kl v D 1 digital 10 Recording Size 0 00 MB 4 4 Hi Res Disk Remaining 442 53 GB gt V A 1 nano2 i gt V B 1 stim Disk Fill Time gt V Gl micro gt V G2 EMG Recording Segment 0 4 LFP ed 00 00 00 gt V A 1 nano2 Elapsed Time 00 00 00 gt V B 1 stim Segment Size 0 00 MB gt V Gl micro 4 V Raw Signal Current Files 0 v A 1 nano2 gt dl B 1 stim s SC micro EI Stop After 30 0 sec J Segor Penning Every T Iw e Recent Files 0 Clear 4 V Spike gt V A l nano2 Save Instrument Configuration Play Tone on Start Stop gt V B 1 stim gt V Gl micro 4 d Stim Digital Triggering J Enabled Valid Trigger Settings hide gt V B 1 stim 5 transition Start SMA 1 eh sl transition Stop SMA 1 v leie Se V pin Pause None ON T Unpause Non z eg Enable Remote Control Figure 3 29 Trellis File Save EI Document Version RO1I550_08 Chapter 3 Trellis Software Suite
20. and reference selection switches are visible on the front panel of the Grapevine Nano Front Ends The Grapevine Nano FEs use a 36 position Omnetics connector to interface with implanted microelectrode platforms Adapter boards may be required to connect to some electrodes A pinout diagram of the connector can be found in Appendix A The 40 pin Omnetics connector has 32 pins for recording electrode connections 2 pins for reference electrodes 2 pins for ground connections and 4 guide pins All of the recording electrodes are referenced to a single reference electrode which is selectable with reference selection switches on the front of the Grapevine Nano style FEs Reference selection is explained in Table 1 4 Note The Grapevine Nano FEs are not intended for clinical or research use with humans Omnetics Bi Lobe ZA to grapevine NIP __ cable to reference grapevine NIP selection P 7 switches reference selection switches Pin 1 indicator cht L bag __Omnetics Connector pin 1 _ Omnetics Connector to electrodes indicator to electrodes Figure 1 10 Front panel of the Grapevine Nano FEs Nano left Nano2 right Reference Selection Switches All of the recording electrodes for the Grapevine Micros Nano and Surf S FEs are referenced to a single reference electrode which is determined by setting the reference selection switches on the Grapevine FE Table 1 4 below explains the reference selection
21. be set from the Trellis Settings window as shown in Figure 3 2 Play tone on Start or Stop When this box is checked a tone will play from the computer when recording is started and stopped Save Instrument Configuration When this option is enabled an instrument configuration file xfg will be automatically created with each data file This file can be loaded at a later time within the Instruments configuration interface to recall the settings of the hardware data streams threshold settings and any hoops placed during online spike sorting Digital Triggering This panel enables the configuration of Trellis to start stop pause and resume file recording when it receives LVTTL inputs from an external source through the Digital I O FE If triggering is being used valid non overlapping LVTTL input values must be specified for the start and stop triggering functions Specifying pause and resume triggering functions is optional and can only be used in conjunction with start and stop LVTTL values can be specified for an individual Digital I O SMA port or a 16 bit hexadecimal pattern on the parallel port If a 16 bit hexadecimal pattern is used for triggering the pattern value must be between 0x0000 and the mask level selected Recording Status During data acquisition the right panel displays the status of the current recording including elapsed time of the recording file size available storage remaining and recently recorded data files
22. cho EL chs chs I cho chs ch ch7 I cre chi0 E cho sees f cho chi0 Ch 12 ee Ch 11 Tt Ch11 GIG Ch 12 chi4 E chs chs Ii ania chi E chis chis cho chis HR chi Ch17 Ch 18 ch20 E cni t Ch 19 E Ch 20 ch22 E MED Ss ch21 L I E ch2 SEA E SE Y hz 1 E ch2 ch26 E cs chas ch26 ch28 E ch27 ch27 CH 2 ch30 E SE ch29 ch30 SEO E c ch31 OO ch32 GND rer2 pe E ano guide pin guide pin guide hole KI ZS guide hole Figure A 1 Grapevine Nano style FE pinout configuration 55 Document Version R01550_08 Appendix A Technical Information ERS Nano PN R00894 Nano Size 31 75 mm x 13 25 mm x 3 5 mm LxWxD Weight 3 5g The Grapevine Nano uses a 4 guide post 36 position Omnetics nanostrip connector PN A79023 001 mates with PN A79022 001 A79024 001 or A79026 001 connectors to interface with electrode platforms using a pin configuration shown in Figure A 1 Because the Grapevine Nano has high input impedance it is suitable for connection to microelectrode arrays and other implanted electrodes To minimize the weight and for small animal use the Nano does not have secondary isolation circuits or shielding Acquired biopotential signals are first filtered in hardware through a high pass filter with a 0 3 Hz cutoff and low pass filter with a 7 5 kHz cutoff The ADC has 16 bit resolution with 0 2 uV bit and an input range of 5 mV Each channel is sampled at
23. digital output pulse is 6 2 ms from the spike threshold crossing event EI Document Version RO1I550_08 Chapter 3 Trellis Software Suite Fast Settle Triggers This panel contains options for configuring the triggers for fast settling of recording FEs This is useful for simultaneous stimulation and recording applications that can have a stimulation artifact in the recording All FEs can be configured together or each one can have a unique trigger set by expanding the AII selection under NIP Front End There are four ways to apply FE fast settling as described below and clicking the question mark next to each trigger type will bring up help information Note Fast settle is available on Grapevine Nano v1 Front Ends with firmware 2 2 only o Digital Input Triggers Use an external digital input connected to one of the four SMA input ports on the Digital I O FE The trigger can be set to when the signal is high or low Each FE will have fast settling enabled for the duration of the digital input trigger and for the specified duration after the digital input trigger o Stim Triggers Use a stimulation pulse from a Micro Stim FE connected to the NIP The fast settling of each FE will occur during the stimulation pulse and for the specified duration afterwards The stimulation trigger can occur on the Any Front End A stimulation pulse on a Micro Stim FE will trigger fast settling of all recording channels on all supported Front En
24. g The Grapevine Surf S uses a 36 pin Samtec connector PN SEMC 118 L1 S D mates with Samtec FTS FISH or TFM series connectors to interface with electrode platforms using a pin configuration as shown in Figure A 2 Because the Grapevine Surf S has high input impedance it is suitable for all surface electrodes Biopotential recordings are first filtered in hardware through a high pass filter with a 0 3 Hz cutoff and low pass filter with a 7 5 kHz cutoff The ADC has 16 bit resolution with 0 2 uV bit and an input range of 6 mV Each channel is sampled at 30 kS s and has an input impedance of 450 MQ in parallel with 15 pF capacitance with input referred noise of less than 2 1 uV RMS All recording electrode channels are referenced to a single reference electrode with selectable configurations see Table 1 4 Note that G in the pinout configuration indicates the ground connections and RI and R2 indicate the Reference and Reference 2 connections EMG PN RO2008 Size 51 5 mm x 36 5 mm x 4 75 mm LxWxD Weight 12 5 g The Grapevine EMG uses a 36 pin Samtec connector PN SFMC 118 L1 S D mates with Samtec FTS FISH or TFM series connectors to interface with electrode platforms using a pin configuration shown in Figure A 3 below The Grapevine EMG is suitable for connection to all low impedance differential surface and implanted electrodes The differential biopotential recordings are first filtered in hardware through a low pass filter
25. high pass filter that is user controlled for the Nano2 0 1 Hz default and fixed at 1 0 Hz for the Nano2 HV and Nano2 Stim followed by a low pass filter with a 7 5 kHz cutoff The Analog to Digital Converter ADC has 16 bit resolution and can be configured to be 0 125 0 25 default or 0 5 uV bit providing an input range of 4 8 or 12 mV respectively At the highest resolution setting 0 5 uV bit the linear range of data is only 12 mV though clipping will not occur until 16 mV there is a non linear gain in all data above 12 mV Each of the 32 channels are sampled at 30 kS s All recording electrode channels are referenced to a single reference electrode with selectable configurations see Table 1 4 Note that G in the pinout configuration indicates the ground connections and R1 and R2 indicate the Reference 1 and Reference 2 connections The maximum safe input voltage is 2 5 V for the Nano2 and 8 5 V for the Nano2 HV and Nano2 Stim The Nano2 HV has additional circuitry for high voltage protection in applications where nearby stimulation may occur Grapevine User Manual Document Version RO1550_08 ES ERS The Nano2 can measure electrode impedances between 1 kQ and 1 MQ and the Nano2 HV and Nano2 Stim can measure impedances between kQ and 400 kQ The Nano2 Stim FE is capable of delivering current controlled stimulation on up to 32 independent electrodes with a range of up to 1 5 mA using 127 discrete current amplitude steps
26. i d XLTEK headbox and a Grapevine Micro recording Front End A Document Version RO1550_08 Appendix E Adapters Screw Terminals for Single Wire Electrodes The Screw Terminal adapters RO1053 RO1217 and RO1407 connect each channel of a Front End including references and ground to a screw terminal for use with single wire electrodes RO1053 and RO1217 are for use with very few electrodes in a small form factor and all unused channels are connected to ground RO1407 can be used with single ended and differential Front Ends and has small switches that can connect unused channels to ground to reduce environmental noise Be sure to mate Front Ends to the adapters so that the pin markers align RO1217 4 Channel Screw Terminal RO1053 is similar but with Omnetics Nano Terminal RO1407 32 Channel Screw Terminal RI d _Electrode d Top Rou Single Ended Bot Row lt Differential Pair gt E ripple ee CH i Spa pu III ww UH LE E Sp D Se LER PSs amp 7 INIA Hie 5 7 9 44 43 46 47 19 21 23 26 27 20 94 Ri Ond 4 6 6 7 O 9 10 44 42 43 44 146 16 XK Gnd gt 4 15 i ADi D _ E s INN NRNR peeeeeen sy J s 4 G i j a d s K gt a zi y i0 MN L i 22 2 26 28 30 2 12 TEC e Za Ae _ i d feeaeeesaat T te 8e8ee8e 8 Bs E S e j _ i i i R d A ES ME Se Se ee fe Use suitches to tie unused connections to Ground Electrode Adapter Rod The Electrode Adapter Rod RO1229 conn
27. or rack with 4 screws not included Digital I O connectors The top panel of the Grapevine Digital I O contains the Micro D connector used to connect the Digital I O FE to the Grapevine NIP via a front end I O cable The bottom panel of the Grapevine Digital I O FE contains a mini RS 232 serial I O port that is currently not operational but will be enabled in a future release The left side panel of the Grapevine Digital I O contains the connectors and ports for digital inputs The Grapevine Digital I O has 4 SMA input ports that accept LVTTL inputs at up to 10 kS s kilosamples per second Each port supports one channel of digital data that is synchronized with data acquired from other Grapevine FEs The Grapevine Digital I O also has a 25 pin Micro D connector that supports 16 bits of digital input on a single cable Ripple supplies a standard 25 pin Micro D to Micro D cable contact Ripple for specialized cabling needs Additionally Ripple produces the Grapevine Digital I O Breakout Box that connects to the 25 pin Micro D port and houses 18 BNC terminals for simple connection of up to 16 digital and two strobe inputs More detailed specifications for this connector including pinout information can be found in Appendix A The right side panel of the Grapevine Digital I O contains the connectors and ports for LVTTL digital outputs The Trellis software suite includes an interface by which the Grapevine NIP can be configured to route spike e
28. package may be found here and is available in x86 and x64 versions Plexon Offline Sorter The Offline Sorter program must first be configured by going to Tools gt Data Import Options gt Cyberkinetics selecting Use Neuroshare Loader and pointing the Neuroshare DLL Location to the correct Trellis Tools thirdparty nsNEV Library xX X nsNEVLibrary dll path Files can then be imported into Offline Sorter as Cyberkinetics files Note Use of these DLL files currently requires the installation of Visual C Redistributable for Visual Studio 2012 This package may be found here and is available in x86 and x64 versions Grapevine User Manual Document Version RO1550_08 70 om Se Appendix E Adapters Ripple provides adapters that connect Grapevine Front Ends to all passive electrodes and electrode arrays as well as adapters for recording and stimulation testing purposes Some of the common Front End adapters are described below along with figures showing how the cables adapters and Front Ends connect together Custom adapters that connect Front Ends to any electrode array can be made by contacting Ripple Samtec Omnetics The two most common array connector types are the Samtec 0 050 36 pin terminal ICS style Utah arrays and the Omnetics 0 025 36 position terminal with 4 guide posts Microprobe FMAs and U V Probes The Samtec to Omnetics adapter RO1804 also known as the micro to nano adapter
29. set of views for the Grapevine Analog I O Front End EES Grapevine User Manual Document Version R0O1550_08 20 ERG Scout Processor The Grapevine Scout is a rack mountable neural processor that is designed for lower channel count animal laboratory research applications The system has built in Analog I O and Digital I O Front Ends FEs and can be configured for up to 64 or up to 128 channels of data acquisition via up to two or four FEs The operation of the Grapevine Scout system is the same as the Grapevine NIP Note The Grapevine Scout is not intended for clinical or research use with humans The Grapevine Scout is not CE approved Front Panel The front panel of the Scout provides the power switch Analog I O and Digital I O Front End connectors and two front end ports that can be connected to up to four FEs depending on the Scout configuration The Analog I O and Digital I O Front Ends that are built into the Scout are the same as the standalone versions described earlier except that there are four BNC connectors for the first four channels of the FE instead of four SMA connectors and the additional breakout channels use a standard 25 pin D sub connector instead of a Micro D connector analog in analog out 1 2 3 4 1 2 22 3 33 4 2 Geh IT W analog group In audio analog group out scout ONA A A od EES lt Ss VH B E d 1 2 34 H HMM EM 24 J ba 8
30. simple ASCII text based format as follows Trellis standard electrode map file Format lt hardware address gt lt elec_group elec_label gt lt view coords gt Example for use with Grapevine NIP nip port FEslot channel array Jelectrode x y z NOTE all fields are 1 indexed H H H RK E E E HHH FH 1 A 1 020 USEA1 elecO01 1 1 1 A 1 022 USEA1 elec002 2 1 1 A 1 024 USEAI1 elec003 3 1 1 A 1 028 USEA1 elec004 4 1 1 A 1 030 USEA1 elec005 5 1 1 A 1 032 USEA1 elec006 6 1 Each row specifies three values 1 A hardware address 2 An electrode label 3 A grid position Values in each row must be separated by semicolons Rows can be specified in any order The hardware address for a channel consists of the NIP ID optional port FE slot and channel number separated by periods The electrode label can be any arbitrary text string If multiple groups of electrodes are present e multiple physical arrays an optional group label can be prepended to the electrode label separated by a period The view position is a set of x y z coordinates separated by periods where the 1 1 1 position is the top left corner of the display An example map file can be downloaded from here EI Document Version RO1I550_08 Chapter 3 Trellis Software Suite ES Spike Scope The Spike Scope application provides a detailed view of a channel s spike waveforms and associated spike processing settings such as thresholds and uni
31. the Preview column Pressing the space bar for stimulation execution can be enabled for quick repetition and or easier control of stimulation the control is located under the play and stop buttons Note Because the Stimulation Safety Switch is its own application it does not close when the Stimulation Controller application is closed or when Trellis is closed e The play button to the right of the Multi Channel Stim display shown in the bottom of Figure 3 27 below is used to execute the multiple electrode stimulation score By selecting one of the two options in the Stim Channels dropdown menu the play button can be used to execute either the entire stim score All or just the patterns for the selected set of electrodes Selected by clicking the electrode labels to the left of the stim patterns The Multi Channel Stim panel includes an option for executing the stimulation score on loop In this mode the Multi Channel Stim player repeats the entire stimulation score or patterns on the selected set of channels for the specified iterations 1 pattern selected to play gt m OOo d Stim When Space Bar Presed Loop Enabled Repeats 1 Stim Channels p Figure 3 27 Trellis Applications Stimulation Controller Stimulation execution controls e B StimKillSwitch Stimulation Safety Switch Figure 3 28 Trellis Applications Stimulation Controller Sa
32. the Grapevine NIP item under the Instrument Network on the left side of the window and select Show Properties as shown in Figure B 4 below lt lt lt lt lt Analog 30k v D 2 analog IO Digital Events v D 1 digital 10 Hi Res v A 1 nano2 v B 1 stim v CL micro v C 2 EMG LFP v A 1 nano2 v B 1 stim v CL micro Raw Signal v A 1 nano2 v B 1 stim v CL micro Spike d Al nano2 gt V B 1 stim gt V CL micro Stim gt V B 1 stim Data Packets Sent H Data Rate Mb s 0 00 Config Packets Sent 1 Config Rate Mb s 0 00 UDP Packets Sent 1 Advanced Options IP4 Network Use localhost Latency Test Start Avg 0 0 ms Max 0 0 ms Gs File Tools Help amp trellis a PG Instrument Network Network Status Active 1 Instrument Online Oss oO z 4 g Grapevine NIP 1 SEH a MH Front Ends e Zen H A 1 nano2 RO0894 0004 v3 2 Network Address 192 168 42 210 8 1 stim RO1289 0005 v2 1 Network Traffic 8 C 1 micro RO0249 0112 v5 2 Data Rate Mbps C2 uE R02008 0001 v2 1 Data Packets Received 4 896 986 reset U D 1 digital IO R00591 0027 v3 1 Data Rate Mb s 30 05 U D 2 analog IO R00407 0021 v3 2 a WW Data Streams Config Packets Received 4 743 4 V Analog 1k Config Rate Mb s 0 00 v D 2 analog 10 UDP Packets Received 366 610 Figure B 3 Instrument
33. the NIP is connected to the computer To run Trellis on Windows double click on the application link that was placed on the computer desktop during installation On Mac navigate to the Trellis subfolder of the Applications folder and click on the Trellis app icon On Linux Trellis can be found in Gnome s list of installed applications Figure 3 1 Trellis desktop icon Windows The main Trellis program presents three tabs on the left side of the window Instruments Applications and File Save that provide access to different aspects of program functionality The color scheme for Trellis and which messages are displayed can be set from the Trellis Settings window as shown in Figure 3 2 These options can be accessed from the Tools menu or from the Settings icon located at the top right of the Instrument Configuration panel shown in Figure 3 5 Zea Trellis Settings General Color Scheme Dark on ight Messages When Loading Config File Prompt Before Restoring Output Tracking Settings V When Loading Config File Prompt Before Restoring File Save Settings V Show message when Invalid Grapevine Software event occurs V Show message when Trellis Error event occurs V Show message when Trellis Network Error event occurs v Show message when Trellis Network Update event occurs V Recording Auto Segment Suffix Number e Close Figure 3 2 Trellis Settings window EI Document Version RO1I550_08 Chapter 3
34. to an active Grapevine NIP Connected The FE is properly connected to the front end cable and Green l the cable is connected to an active Grapevine NIP Orange Initialization or Error Front End to Electrode Connector Grapevine Micros Surf S and EMG The bottom panel of the Grapevine FE contains the 36 pin Samtec connector for interfacing with electrode platforms Adapter boards may be required to connect some electrodes A pinout diagram of the 36 pin Samtec connector can be found in Appendix A The 36 pin Samtec connector on the Grapevine Micros Surf S and EMG has 32 pins for recording electrode connections 2 pins for reference electrodes and 2 pins for ground connections All of the recording electrodes are referenced to a single reference electrode which is selectable with reference selection switches on the back of the Grapevine FE Refer to Table 1 4 for information on selecting references The 36 pin Samtec connector on the Grapevine EMG has 32 pins for recording 16 pairs of electrodes and 2 pins for ground connections Each of the recording electrodes is paired with a unique reference electrode which is specified in the Grapevine EMG pinout diagram found in Appendix A Samtec Connector to electrodes Figure 1 7 Bottom panel of the Grapevine FE Micros Surf S and EMG Grapevine User Manual Document Version R01550_08 Eva p lt SS Front End Front Panel Grapevine Micros Surf S and EMG The fron
35. 0 103 4 S LALO6 1000 10 100 103 3 A 1A107 1000 10 100 101 2 1A108 1000 10 100 102 3 LALOS 1000 10 100 102 4 1A110 1000 10 100 102 3 alt Lal 1000 10 100 10 2 5 Medium 1A112 1000 10 100 101 5 Hig 1A113 1000 10 100 103 4 1A114 1000 10 100 102 4 1A115 1000 10 100 102 5 1A116 1000 10 100 101 3 La 1000 10 100 102 6 1A118 1000 10 100 103 4 1A119 1000 10 100 102 4 1A120 1000 10 100 10 2 5 1A121 1000 10 100 101 4 1A122 1000 10 100 103 5 1A123 1000 10 100 102 4 1A124 1000 10 100 103 5 1A125 1000 10 100 103 5 E Loo Save Append Copy to Cipboard ear Figure 3 18 Trellis Applications Impedance Analyzer completed test with results The impedance data may be saved in three ways 1 The results may be saved to a file using a white space delimited row format 2 The data may be appended to a previously saved file 3 The data may be copied to the clipboard to be pasted into third party software Electrode map files can be used in the Impedance Analyzer To load a map file switch the browser panel on the top left to Electrode View and then click on the browse button This will allow the impedance results to be sorted in electrode order The electrode map file must be loaded before starting the impedance run Impedance test results can be sorted by clicking on any column header in the Impedance Results panel To make changes to the Electrode Selection panel the Impedance Results panel must be cle
36. 30 kS s and has an input impedance of 450 MQ in parallel with 15 pF capacitance with input referred noise of less than 2 1 uV RMS All recording electrode channels are referenced to a single reference electrode with selectable configurations see Table 1 4 Note that G in the pinout configuration indicates the ground connections and R1 and R2 indicate the Reference 1 and Reference 2 connections The maximum safe input voltage is 5 V The Nano can measure electrode impedances between 1 KO and 1 MQ Note The Grapevine Nano is not intended for clinical or research use with humans Micro2 Micro2 HV Micro2 Stim PN RO2004 Micro2 RO2005 Micro2 HV RO2006 Micro2 Stim Size 30 mm x 26 mm x 4 75 mm LxWxD Weight 9 g Micro2 9 5 g Micro2 HV and Micro2 Stim The Grapevine Micro2 Micro2 HV and Micro2 Stim use a 36 pin Samtec connector PN SFMC 118 L1 S D mates with Samtec FTS FTSH or TFM series connectors to interface with electrode platforms using a pin configuration shown in Figure A 2 Because the Grapevine Micro2FEs have high input impedance they are suitable for connection to microelectrode arrays and other implanted electrodes Acquired biopotential signals are first filtered using a high pass filter that is user controlled for the Micro2 0 1 Hz default and fixed at 1 0 Hz for the Micro2 HV and Micro2 Stim followed by a low pass filter with a 7 5 kHz cutoff The Analog to Digital Converter ADC has 16 bit resolution and c
37. IP by multiple computers running Trellis software and or other NIP control software Auxiliary Port The auxiliary port is used to synchronize multiple Grapevine NIPs to create a greater than 512 channel recording and stimulation system Contact Ripple for questions on how the auxiliary port may be used for unique applications Expansion Ports The Grapevine NIP is fitted with two USB type expansion ports for controlling external devices prosthetic limb pump computer cursor etc When delivered these ports on a Grapevine NIP are not operational but can be modified by Ripple to drive other digital outputs or directly save data to a disk without the use of a computer system For more information on this contact Ripple directly Due to power limitations only devices that have been explicitly approved by Ripple can be safely connected to the expansion ports on the Grapevine NIP Bottom Panel The bottom panel of the Grapevine NIP provides the front end ports to attach Grapevine FEs front end front end front end front end portA port B port C port D Figure 1 3 Bottom panel of the Grapevine NIP Front End Ports There are a total of four front end ports that can each be connected to up to four Grapevine FEs via single double triple or quadruple front end cables In total the four ports enable a single Grapevine NIP to support up to sixteen Grapevine FEs Note Currently only one Analog I O FE and one Digital I O FE can be connect
38. ISISIE SISI g E felele SISS SISS Figure 3 5 Trellis Instruments Grapevine NIP configuration 29 Document Version R01550_08 Chapter 3 Trellis Software Suite ES Trellis automatically detects which Front Ends FEs are connected to the Grapevine NIP and displays the appropriate configuration panels as shown in Figure 3 5 Each configuration panel can be expanded or collapsed by clicking the or symbol to the right of the panel title e Data Streams This panel provides the ability to enable disable the various data streams associated with each FE that is connected to the NIP Each data stream consists of a series of data packets that are broadcast by the NIP onto the instrument network via UDP The data streams and their properties are o Analog Input streams can be enabled for the Analog I O Front End at 1 kS s per channel Analog 1k and or 30 kS s per channel Analog 30k o Digital Input streams can be enabled for the Digital I O Front End at 10 kS s by selecting the Digital Events data stream o Hi Res streams contain continuous waveform data with 24 bit floating point precision allowing for better precision at the low end of the spectrum and wider dynamic range which can be useful for ECoG and EMG data The Hi Res signals are sampled at 2 kS s and are digitally filtered using a low pass IIR filter of 500 Hz with 4 order Butterworth characteristics o LFP streams contain continuous wavef
39. KR Beerereeerrerer cit era ttre een mente eRrnne E a nme 59 Eer EE 60 Discontinued Front EndsS s ssssssssssunnunnnnnnnnnnnnnunnunnunnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn mannana 61 LEE eeh 61 Di Document Version RO1550_08 Contents APPENDIX B SYSTEM INFORMA TION Eege EEN 62 Trellis SoftWare srine eegene 62 Grapevine Processors and Front EndS s sssssususnunnnnnnnnnnnnnnnnunnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 63 APPENDIX C HARDWARE amp SOFTWARE CONFIGURATION ssssscccceeeceeeseecnssssnsseneneeeeeeeeeeeeseenenessssaeagggnnes 66 Hardware sen decade can ev tacca cast easeaiacts cd astuaucecavaudcasaucubuacsesudvettuenstasanivaeesines 66 Trellis Software Suite ississccesiceccceticsieed cite dexsaicevancsscuncaxtiezedseiaudexeticsveassasececutiicwatessantentavseanseabinns 67 Trelis Gontiguration File sinistral acing 68 APPENDIX D ADDITIONAL SOFTWARE COMPONENTS vssssssssscccceeeeeeeeeeseennenssssaeeaegeneeeeeeeeeeeeecenessssnaaggggens 69 ENKE 69 Open Source MA RRE 69 Oe fc One EE 69 UE 69 EE 69 PY CH OR E 70 Ee 70 Third Party Software Compatibility sssusussnsusunnnnununnnnununnnnununnnnununnnnununnnnununnnnunannnnnnnnnnnnnnne 70 Nex Technologies Tee e eg 70 Plexo OTS Sorter Vesien E 70 APPENDIX EADAPTERS geet 71 SAINTE C O MINING NCS cinan E a 71 Front End Cable Saar 71 TOUCH POOL ana eee ncaa ere anne eee 71 PMU EE 71 Screw Terminals for Single Wire Electrodes ssssssssussssnnusunnnnununnnn
40. Network File Tools Help amp trellis a PO Instrument Network Or Sie 3 Figure B 4 Accessing Grapevine NIP properties 63 Document Version RO1550_08 Appendix B System Information BR Grapevine NIP 1 a Mu Front Ends A 1 nano a WW Data Streams gt Analog OK gt dl Analog 30k gt Diaital Events B 1 stim ROT C 1 micro ROO249 0112 v5 2 C 2 EMG RO2008 0001 v2 1 D 1 digital 10 RO0591 0027 v3 1 D 2 analog 10 ROO407 0021 v3 2 Load Channel Map Show Properties ES This will enable the display of all properties of the NIP and connected FEs as individual modules as shown in Figure B 5 below The first module module 0 is the Grapevine NIP The Grapevine Front End amplifiers will be listed as modules 1 32 with 2 consecutive modules listed for each FE The Grapevine Analog and Digital I O FEs begin at module 33 The properties of the NIP and all connected FEs including the hardware version can be found by clicking on the module as shown in Figure B 6 through Figure B 8 File Jools Help trellis Gers a TG Instrument Network S a H Grapevine NIP 1 gt Ku Front Ends ZiT gt WWW Data Streams 4 Properties gt module 0 33 properties module 1 324 properties module 2 670 properties module 3 324 properties module 4 670 properties module 5 324 properties module 6 670 properties module 7 90 properties module 8 129 properti
41. al and the maximum allowable parameter value can be set by moving the slider bar to the right of the dial Phase 1 Duration Increment usec 1 10 100 Figure 3 22 Trellis Applications Stimulation Controller Parameter dial Grapevine User Manual Document Version RO1550_08 46 p lt SS Descriptions of the various stimulation pattern parameters and their defaults are as follows e Offset This control specifies the time in milliseconds from the beginning of the stimulation period to when the leading phase of the stimulation pattern begins The default for this parameter is U ms and in most cases it will not need to be changed Interleaved stimulation across multiple electrodes is an example of a stimulation paradigm where the offset parameter is used For a given stimulation period the maximum offset is the period minus the length of the stimulation waveform e Phase 1 These two controls specify the parameters of the leading phase of the stimulation waveform The default amplitude is three times the stimulation step size specific to the FE and the default duration is 100 us The maximum amplitude is 127 current steps 950 uA for the 7 5 uA step sized stim FE shown and the maximum duration is 50 ms The duration resolution is 1 us e Phase Ratio This control specifies how the amplitude ratio and the duration ratio between the two phases of stimulation are implemented The choices are Open 1 1 1 2 1 4 and 1 8 A p
42. an IP address automatically Use the following IP address IP address 192 168 Subnet mask ebe Default gateway Obtain DNS server address automatically Use the following DNS server addresses Preferred DNS server Alternate DNS server Validate settings upon exit Cas Cere Figure 2 4 Step 5 Correct network adapter settings for IPv4 The computer will able to receive data from the Grapevine NIP with this configuration However firewalls and other network security settings may interfere with the communication resulting in an inability to collect data or possible data loss To ensure that the system functions properly disable all firewalls and network security involving the network card connected to the Grapevine NIP Grapevine User Manual Document Version RO1550_08 2A ERS Disabling the Windows Firewall Step 1 Open Control Panel Click on the Windows button and then Control Panel to open the Windows 7 Control Panel Step 2 Open Windows Firewall Settings Viewing by Large Icons click on Windows Firewall to access the Firewall settings Step 3 Open Advanced Firewall Settings Click on Advanced Settings on the left side of the Windows Firewall panel Step 4 Open Windows Firewall Properties Click on Windows Firewall Properties in the middle of the Windows Firewall with Advanced Security window In this window each of the profiles must be changed to enable data str
43. an be configured to be 0 125 0 25 default or 0 5 uV bit providing an input range of 4 8 or 12 mV respectively At the highest resolution setting 0 5 uV bit the linear range of data is only 12 mV though clipping will not occur until 16 mV there is a non linear gain in all data above 12 mV Each of the 32 channels are sampled at 30 kS s All recording electrode channels are referenced to a single reference electrode with selectable configurations see Table 1 4 Note that G in the pinout configuration indicates the ground connections and R1 and R2 indicate the Reference 1 and Reference 2 connections The maximum safe input voltage is 2 5 V for the Micro2 and 8 5 V for the Micro2 HV and Micro2 Stim The Micro2 HV has additional circuitry for high voltage protection in applications where nearby stimulation may occur The Micro can measure electrode impedances between 1 KQ and 1 MQ and the Micro2 HV and Micro2 Stim can measure impedances between 1 kQ and 400 KQ The Micro2 Stim FE is capable of delivering current controlled stimulation on up to 32 independent electrodes with a range of up to 1 5 mA using 127 discrete current amplitude steps and a compliance voltage of 8 5 V The discrete current step size can be configured to be 1 2 5 10 or 20 uA step 75 steps for the highest step size All stimulation is in reference to the ground Grapevine User Manual Document Version RO1550_08 Ea ERS connection Stimulation and recor
44. ardware and Trellis software This Configuration file can be saved through either the main Trellis Instruments program or the File Save Application and can be restored through the main Trellis Instruments program Besides storing all of the above hardware and software settings the Trellis Configuration File also contains information about per channel single unit spike sporting Application window sizes locations and Trellis main options such as notification selections and color scheme Grapevine User Manual Document Version R01550_08 68 om Appendix D Additional Software Components MATLAB Ripple has developed an online MATLAB interface to Trellis and the Grapevine NIP called Xippmex This software enables instrumentation configuration of the Grapevine NIP and near real time access to data streams and stimulation output as well as control of recording with Trellis Ripple is currently providing this code in a beta version that fulfills a subset of the capabilities Xippmex is located in the installed directory Ripple Trellis Tools xippmex There is a Xippmex user manual located on the website and in the Trellis document directory at Ripple Trellis Documentation xippmex that includes examples on how to use the software components Open Source MATLAB Ripple provides open source offline MATLAB functionality with the Trellis software suite These interfaces functions and associated examples can be found i
45. ared using the Clear button in the bottom right A3 Document Version RO1I550_08 Chapter 3 Trellis Software Suite ES Stimulation Controller The Stimulation Controller is used to configure the stimulation parameters for Micro Stim Micro2 Stim and Nano2 Stim FE channels referred to as Stim Front Ends throughout the rest of this section Single pulse pulse train and continuous stimulation patterns can be set for each electrode Biphasic phase balanced cathodic leading stimulation is the default pulse shape but this can be customized using the controls described in this section When the application is started the Stimulation Hardware panel in the top left will display all available Stim FEs and their channels All other FEs which do not support stimulation will be grayed out as shown in Figure 3 19 The stimulation current step size of each Micro Stim must be entered when a Micro Stim is loaded in the Stimulation Controller app for the first time as shown in Figure 3 20 For Micro2 Stim and Nano2 Stim Front Ends this current step size is user controlled and set in the Hardware Description Panel described later in this section After expanding the micro stim front end entity in the Stimulation Hardware panel and selecting an available electrode a configuration display will appear as seen in Figure 3 21 This display provides the Front End hardware information and enables the configuration of various stimulation
46. arted and FEs that do not support impedance measurement will be grayed out as shown in Figure 3 17 The following Front Ends support impedance measurement e Grapevine Micro Front End firmware versions 3 2 4 2 or 5 2 e Grapevine Nano Front End firmware versions 2 2 or 3 2 e Grapevine Nano2 Nano2 HV Nano2 Stim Micro2 Micro2 HV and Micro2 Stim Front Ends all firmware versions r Impedance Analyzer TM aE So File Tools Help Electrode Selection Test Parameters Impedance Results Hardware View Electrode View Frequency Hz Pin Frequency Hz Current nA Cycles Magnitude kOhms Phase E 1A101 1000 10 100 4 7 Grapevine NIP XippID 1 1000 ENEE 1A102 1000 10 100 V micro front end Current nA 1A103 1000 10 100 4 d Por B Nanos 3 nA only 1A104 1000 10 100 d nano front end 3 LALOS 1000 10 100 z S 4 geg es oa 10 1A106 1000 10 100 PotD 30 1A107 1000 10 100 1o E 100 1A108 1000 10 100 1A109 1000 10 100 Accuracy 1A110 1000 10 100 I Fast 1A111 1000 10 100 Medium 1A112 1000 10 100 Highest 1A113 1000 10 100 1A114 1000 10 100 1A115 1000 10 100 1A116 1000 10 100 1A117 1000 10 100 1A118 1000 10 100 1A119 1000 10 100 1A120 1000 10 100 1A121 1000 10 100 1A122 1000 10 100 1A123 1000 10 100 1A124 1000 10 100 1A125 1000 10 100 Start H Pause H Cancel NN Save Append Figure 3 17 Trellis Applications Impedance Analyzer Recording electrode impedances are measured using a standard 1 kHz
47. ate is only captured when either Strobe A or Strobe B is set high The setting can be changed in either Trellis or by using Xippmex so that the 16 bit parallel port state is captured when there is a bit change on any of the parallel port lines The Grapevine Digital I O FE uses LVTTL for signal transmission meaning that a low voltage or 0 bit is from 0 V to 0 8 V whereas a high voltage or 1 bit is from 2 V to 3 3 V Note the Digital I O FE can handle up to 5 V inputs In order to be recognized by the system digital input events must span at least 2 clock cycles 1 e a bit must be low or high for at least 67 us The digital inputs have an impedance of gt 10 MQ and are sampled at 10 kS s Digital Outputs The Grapevine Digital I O is capable of producing up to 20 digital outputs Using the Trellis software spike events can be configured to trigger a LVTTL pulse on a digital output channel On the right side of the Grapevine Digital I O are 4 SMA connectors and a single 25 pin Micro D connector for output use Each SMA connector provides a single channel of digital output whereas the Micro D connector provides 16 bits of digital output The remaining pins are used for ground connections and Strobe synchronization Details of the pinout configuration can be found in Figure A 4 above same configuration as Digital I O input The Grapevine Digital I O uses LVTTL for signal transmission 0 3 3 V The digital outputs have an impedance o
48. ble signals collected with the Grapevine NIP to be routed to external devices Additionally the Grapevine Analog I O FE comes with a single stereo audio input port and two stereo audio output ports All of the grounds on the Grapevine Analog I O are tied together internally to minimize grounding issues For detailed specifications of the Grapevine Analog I O Front End see Appendix A The front panel of the Grapevine Analog I O contains markings and labels that identify the FE as well as labels indicating the function of each port The Grapevine Analog I O comes with four mounting holes that allow the device to be mounted to a wall or rack using 4 screws not included Analog I O Connectors The top panel of the Analog I O FE contains the Micro D connector used to connect the Analog I O FE to the Grapevine NIP via a front end I O cable The bottom panel of the Grapevine Analog I O provides three 0 25 stereo audio jacks The leftmost of these jacks is used for 2 channels of audio input that are acquired by the Grapevine NIP in sync with data being acquired from other Grapevine FEs The other two jacks provide 4 channels of audio output 2 channels on each jack This allows for up to four channels of spiking activity to be played from attached speakers The left side panel of the Grapevine Analog I O contains all of the connectors and ports for analog inputs The Grapevine Analog I O has 4 SMA analog input ports and a 25 pin Micro D connector to
49. can be used to connect the larger Front Ends Micros Surf S to electrode arrays that use the Omnetics nanostrip connector The Omnetics to Samtec adapter RO1050 also known as the nano to micro adapter can be used to connect the smaller Nano style Front Ends to electrode arrays that use the Samtec 36 pin connector Be sure to mate Front Ends to the adapters so that the pin 1 markers align RO1804 Samtec to Omnetics RO1050 Omnetics to Samtec III DUU DEER lf A2 ol OASTwW GU Hmmm Front End Cable Saver The Front End Cable Saver adapter RO1610 can be used when connecting a front end cable to fewer Front Ends than the cable requires The adapter effectively takes the place of an active Front End allowing the port cable and Front Ends to be initialized correctly Touchproof The Grapevine Touchproof adapter RO1375 RO1376 provides an interface between Front Ends and electrodes that terminate in 1 5 mm touchproof connectors Adapter RO1375 can be used with 32 channel single reference Front Ends while adapter RO1376 can be used with 16 pair differential Front Ends The latest version of the Touchproof adapter has switches along the bottom that allow the user to ground unused channels EMU The Grapevine EMU 128 adapter RO1273 provides a dual connection for 32 channels of EEG ECoG electrodes to a Grapevine processor and an XLTEK headbox The EEG input signals are passed thru one passive DB 37 port to the
50. communication IIR LP Filter 4th 6th Order BW IIR Notch Filter 2nd Order BW LFP Data Stream 1 kHz Sampling Digital Data IIR Low Pass Filter to Hi Res 250 Hz Default 4th Order BW Data Stream IIR Notch Filter 2nd Order BW Spike Data Stream 30 kHz Sampling 52 Sample Window IIR HP Filter 250 Hz Default Current Controlled Stimulation Bi phasic default Waveforms Micro Stim amp Nano2 Stim Stim Controller Control Stimulation Parameters Micro Stim Figure 1 1 Schematic of the Grapevine system workflow Document Version RO1550_08 Chapter 1 Hardware Overview Hardware Requirements The Grapevine system has the following hardware requirements for any computer that is intended to stream visualize and record up to 512 channels of data Minimum Requirements Processor Intel Core2Duo or Equivalent Memory 4 GB RAM or greater Network Interface Card 1 GB Adapter with large buffer At least 48 kB per port On Chip Memory Recommended Components Internal Storage 2 TB or greater External Storage One or more 2 TB or greater eSATA external SATA drive s Operating System Requirements The Grapevine Trellis software suite is designed to run on Microsoft Windows 7 Apple Macintosh Mac OSX 10 7 10 9 and Linux Ubuntu 12 04 64 bit platforms Understanding Grapevine System Components Neural Interface Processor NIP The Grapevine NIP is the central component of every Grapevin
51. cording FEs perform filtering amplification and analog to digital conversion for the signals that they collect The Analog I O FE 1s capable of acquiring and producing 5 V analog signals The Digital I O FE is capable of acquiring and producing LVTTL data A total of 16 Grapevine FEs can be interfaced with a single Grapevine NIP enabling up to 512 channels of data streaming recording and stimulation Each of the Grapevine FEs and their function are explained below All technical specifications of these FEs can be found in Appendix B Nano Style FEs The Grapevine Nano Front Ends record from up to 32 electrodes These Front Ends have a small form factor and low weight making them suitable for all animal applications including small rodents The Nano2 Stim also delivers current controlled stimulation simultaneously with recording through up to 32 high impedance microelectrodes The Nano2 HV is similar to the Nano2 and includes additional circuitry for high voltage protection in applications where nearby stimulation may occur Note The Grapevine Nano FEs are not intended for clinical or research use with humans Micro Style FEs The Grapevine Micro Front Ends record from up to 32 electrodes They are designed for recording through microelectrode arrays micro ECoG grids and other peripheral and cortical neural electrodes The Micro Stim and Micro2 Stim also deliver current controlled stimulation simultaneously with recording through up to
52. cs The Grapevine Neural Interface System goes through a startup routine every time it is powered on to verify that the processor and all Front Ends are working correctly Ripple recommends that the processor be sent in once a year for a full diagnostic check up by Ripple engineers There is no cost for the diagnostic check up outside of normal shipping costs Ripple recommends that users keep all original Grapevine packaging for this purpose Return Merchandise Authorization RMA Process Ripple Grapevine products that require maintenance or repair should be returned to the address below only after receiving a Return Merchandise Authorization number from Ripple Support support rppl com or 1 801 413 0139 Grapevine components should be shipped to Ripple in secure anti static padded packaging Ripple recommends that users keep all original Grapevine packaging in case of repair or maintenance needs Ripple 2056 South 1100 East Salt Lake City UT 84106 USA Grapevine User Manual Document Version RO1550_08 iv Contents CHAPTER HARDWARE OVERVIEW ee a sceledactic ners a aa a aa Aaa 7 How to Heesprenger 7 Grapevine Neural Interface System sss sssussssueununnnnununnnnununnnnununnnnununnnnunannununnnnnnunnnnnnnnnnnnnnnnn annan 7 Hardware REG ui renin een 8 Operating System Requirements sssssssussnsunsunnunnunnunnnnnnnunnunnunnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnn nanmanna 8 Understanding Grapevine System COMponentsS ssssssssussussssusunnunu
53. ding can be done simultaneously with the Micro2 Stim FE The stimulation output can be calibrated to ensure the delivered current is within 5 of desired stimulation levels see the Instruments section of Chapter 3 for details on running calibration Note There is a known issue with the Nano2 Stim FEs where simultaneous stimulation on multiple electrodes causes distortion in the stimulation output in the scenarios below The solution to this is to use a higher stimulation step size For example if you want to stimulate on 8 channels simultaneous at a level of 20 uA the recommended step size would be 5 uA step This issue only occurs during simultaneous stimulation across multiple electrodes not during interleaved or off set multiple electrode stimulation and does not occur for the 20 uA step step size Number of Simultaneous Channels C Le Cs GZ Le Ge bes bes Lz Lo Lee ee Les Lez Lee ed fed Lel Figure A 2 Pinout configuration of the Grapevine Micro and Surf S FEs Micro Micro HV and Micro Stim PN R00249 Micro RO1425 Micro HV R00894 Micro Stim Size 51 5 mm x 36 5 mm x 4 75 mm LxWxD Weight 12 5 g Micro 13 5 g Micro HV and Micro Stim The Grapevine Micro Micro HV and Micro Stim use a 36 pin Samtec connector PN SFMC 118 L1 S D mates with Samtec FTS FTSH or TFM series connectors to interface with electrode platforms using a pin configuration shown in Figure A 2 Because the Grapevine Micro M
54. ditional data file information can be found from File gt File Info MKsort MKsort is an offline spike sorter created by Matt Kaufman while he was at Stanford University This software enables users to import electrophysiological data and classify waveforms as coming from different units sort spikes It also provides tools for examining the stability of these isolations over time rating the isolations measuring spike widths and examining the functional tuning of the sorted units The main function mksort m and the complete set of functions can be found in the Tools mksort directory A walkthrough document using example data is provided in the Tools mksort docs directory This tool may be found online as well github link 69 Document Version RO1550_08 Appendix D Additional Software Components ES Python Ripple provides offline Python functionality that is included with the Trellis software suite Inside the Tools directory is a directory labeled pyns containing a set of functions and classes based on the open source Neuroshare API which can be used to extract digital and analog data acquired from any of the Front Ends Example command line Python functions can be found in the pyns examples directory Complete documentation on installation examples and module definitions can be found in the pyns pdf file in the Trellis Documentation pyns directory C A set of C headers fo
55. ds Same Front Port A stimulation pulse on a Micro Stim FE will trigger fast settling of all recording channels on all supported Front Ends connected to the same NIP front end port as the Micro Stim FE Same Front End A stimulation pulse on a Micro Stim FE will trigger fast settling of all recording channels on only the Micro Stim FE that produced the stimulation o Auto Trigger All channels for a Front End will automatically be fast settled if any one channel reaches saturation The duration of the auto trigger fast settle is specified by the digital input trigger time Note If this option is enabled in a situation where one or more channels on a Front End frequently saturates e g if a channel is disconnected the recordings on the good channels will likely be compromised due to frequent triggering of the fast settling behavior o Manual Trigger Click this button to fast settle all channels of a Front End The duration of the manual trigger fast settle is specified by the digital input trigger time Filter Settings This panel provides the FE digital signal processing filter settings for all appropriate FEs Each FE is noted by its Front End ID The specific cutoff frequencies for each filter type can be changed by double clicking on the values as shown in Figure 3 6 Filter characteristics can be set for all FEs by clicking on the filter type e g HiRes Filter These digital filters are IIR filters with 4 order Bu
56. e 1 duration is a multiple of the 30 kHz NIP state machine clock cycle 33 3 us E g if the Phase 1 duration is set to 210 us the minimum allowable IP Interval is 23 us An IP Interval of zero is possible if the Phase 1 duration itself is a multiple of the clock cycle e g if the Phase 1 duration is 100 us e Frequency This field specifies the frequency in Hertz of the pulse train when more than one pulse is desired The maximum allowable frequency is 1 kHz If the offset is non zero a maximum bound will be set for the frequency Specifically the frequency cannot be higher than that at which the period equals the sum of the offset and pulse duration If using a burst of stimulation see Duration parameter on the following page and the duration of the stimulation train is not an integer multiple of the stimulation period 1 frequency a warning will be displayed next to the Frequency parameter as shown in Figure 3 23 and the train will have an empty partial period at the end of the pattern noted by a red box in the Train Display The warning text can be refreshed by clicking on the warning symbol A A7 Document Version RO1I550_08 Chapter 3 Trellis Software Suite ES e Duration This sets the total time of stimulation in milliseconds for the current stimulation pattern The choices are o once only a single pulse will be delivered o burst multiple pulses will be delivered at a selected frequency for a set amount of time
57. e screen and then System Preferences to open the Mac OS X System Preferences window Step 2 Open the Network panel Click on the Network icon listed under the Internet amp Wireless section of the System Preferences window Step 3 Select the Network Interface Card connected to the Grapevine NIP On the left side of the Network window there is a list of the available network interfaces that exist on the Mac computer The Grapevine NIP will be connected to one labeled Ethernet or Ethernet Adaptor depending on the computer Click on the network interface that is connected to the Grapevine NIP to show its settings These settings will appear on the right side of the window 25 Document Version RO1550_08 Chapter 2 Installation and Setup ES Step 4 Configure the Network Interface Card connected to the Grapevine NIP First click the dropdown to change the Configure IPv4 field to Manually This should allow for changes to the IP Address field In the IP Address field enter the value of 192 168 42 129 with a subnet mask of 255 255 255 0 Leave the rest of the fields blank Step 5 Configure the necessary Advanced Settings In the Network panel select the appropriate Network Interface Card click on the Advanced button in the bottom right of the screen to access the advanced settings Click on the Ethernet tab and change the values as follows e Configure Man
58. e system Up to sixteen Grapevine Front Ends FEs can be connected to each Grapevine NIP This section describes the different connectors indicators and features of the Grapevine NIP Top Panel The top panel of the Grapevine NIP provides terminals and ports for interfacing with a power supply UDP network expansion ports and auxiliary control interfaces The top panel is also where the power button can be found power power ethernet auxiliary expansion button socket port port ports Figure 1 2 Top panel of the Grapevine NIP Grapevine User Manual Document Version RO1550_08 EES ERS D Document Version RO1550_08 Chapter 1 Hardware Overview Power Connections The power button on the left of the top panel toggles the power status of the Grapevine NIP The 4 pin LEMO circular power socket is used to connect the Grapevine NIP to an isolated medical power supply that is provided with the Grapevine NIP The power supply uses a 3 pin IEC power socket to connect to a 3 pin earthed grounded power cable The power supply can be used in all common international main power supplies between 100 240 V AC at 50 60 Hz Ethernet Port The Ethernet port connects the Grapevine NIP to a computer This enables the NIP to transmit data being acquired from the FEs to the Trellis software as well as control signals from the Trellis software to be sent to the Grapevine NIP A network switch may also be used to enable control of a single N
59. e view of Grapevine signals Spike Grid Vendor Ripple OG Spike Scope Impedance K L Stim Figure 3 7 Trellis Applications Raster The Raster application provides a raster based view of all data streams from the Front Ends FEs Double clicking on an individual channel launches the Spike Scope application providing a detailed view of the spike event activity for that channel E Raster ge E LS ees File Tools Help E kv amp Wh ES Za v WwW MA s Mics L A te SZ wm E s K s0u v We AM W M Time window sec 3 88 UM Processor ID 1 Analog 1k analog 10 D 1 Analog 30k analog IO D 1 Digital Events digital IO D 2 Hi Res EMG A 1 micro B 1 nano B 2 stim C 1 LFP micro Bi nano B 2 stim CL Raw Signal micro Bi nano B 2 stim C l Spike micro B 1 nano B 2 stim C 1 SpkPreview micro B 1 nano B 2 stim C 1 Stim stim C 1 elec B 1 08 hi res spkfilt Figure 3 8 Trellis Applications Raster The Raster application contains a variety of controls to customize the view A graphic and description of each control is provided below H Di GN Scroll colors Ss e Ee Lafe AT LAW 30px 4 500uv Browse available signals and select which ones to display in Raster Group lines by signal type e spike Hi Res LFP raw stim Group lines by FE input channel Overlay signals from the same FE input channel on one line Turn signal clipping on
60. e you can use it Enter the step size in pA for front end R01289 0005 v2 1 7 5 E File Tools Help Le Stimulation Controller X Stimulation Hardware Stim Parameters elec B 1 14 Pattern 1of1 New Delete Selected Delete All Hardware View Electrode View F Waveform elec B 1 14 x ps Waveform oPh asic Defaults 4 Grapevine NIP XippID 1 a Port A Offset 0 msec pees front end Ew pen periem stim front en a Phase 1 4 100 Amp 2 Amp 1 elec B 1 01 J d 1 1 H elec B 1 02 Phase 2 E pa A IP Interval 100 usec elec B 1 elec B 1 05 Frequency 10 00 Hz elec B 1 06 elec B 1 07 Duration once elec B 1 08 burs 1000 msec elec B 1 09 elec B 1 10 E elec B 1 11 elec B 1 12 elec B 1 13 Hardware Description Phase 1 Amplitude Port B Front End 1 R Num R01289 Serial No 0000 Hw Rev 2 Sw Rev 1 Current Step Sze 7 5 uA Apply WS Increment Stim Configurations B Current File untitled stm 1 pattern selected to play Period 100 00 msec 10 00 Hz v Stim Patterns P v Stim Score l Stim When Space Bar Presed Save Train 1000 msec 10 pulses 1600 msec Saved Files Browse 5 mea S Ges E Multi Channel Stim A2 Dir C Users Mitch Dropbox Ripple Grapevine Trellis Demos 32chanWalkingStim_portB2 stm Loop Enabled Repeats 9999 Multi Channel Stim Channels gt m Figure 3 21 Trellis Applications Sti
61. eaming over the network to and from the Grapevine NIP These are configured in each of the tabs listed in the window including the Domain Profile Private Profile and Public Profile Step 5 Configure the Domain Private and Public Profile In the Domain Profile tab click on the Customize button next to the label that reads Protected Network Connections In the window that appears uncheck the NIC to which the Grapevine NIP is connected Click OK to exit this window and apply the settings Repeat this step for both the Private Profile tab and the Public Profile tab Click on the OK button of the Windows Firewall Properties window to apply all of the settings After completing Step 5 the Windows Firewall will be disabled for the network card that connects the computer to the Grapevine NIP Third party antivirus software such as Norton Antivirus and McAfee Antivirus may contain firewalls that can interfere with the communication with the Grapevine NIP These should also be disabled Also virus scanners should be disabled when recording data Mac OS X Network Configuration Configuring the Grapevine NIP to work with a Mac computer running OS X can be done completely through the System Preferences Network Panel no firewall settings need to be changed Configuring the Network Interface Card connected to the Grapevine NIP Step 1 Open System Preferences Click on the Apple icon in the top left hand side of th
62. ects to many adapters through three small screws allowing the user to hold the adapter in i place with a clamp similar to a chem stand rod clamp Analog I O and Digital I O Breakouts The Grapevine Analog BNC Breakout adapter RO1124 converts the 24 input or output channels from the micro D port of the Analog I O front end to standard BNC terminals The Grapevine Digital BNC Breakout adapter RO1396 converts the 16 digital input or output channels from the micro D port of the Digital I O front end to standard BNC terminals The 16 channels of the Digital I O can be configured as independent channels a 16 bit bus with strobe or two 8 bit busses with individual strobes RO1124 Analog I O BNC Breakout RO1396 Digital I O BNC Breakout Grapevine User Manual Document Version RO1550_08 Ei ERG Recording Front End Testing For recording Front Ends a known signal e g from a Function Generator may be used as a test input to ensure that all Front End channels are working correctly Part number RO1404 SMA to 36 pin Samtec uses a 1000 1 voltage divider and connects the input signal to all 32 recording channels using either reference selection The input test signal is stepped down by a factor of 1000 to ensure the safety of the recording amplifier e g a 5 V sine wave input will be recorded as a 5 mV sine wave There is also a differential version available RO1405 for use with differential Front Ends such as the Grapevine EMG
63. ecurity Essentials Erase the previously installed Trellis directory Either from Applications Trellis or Users lt username gt Applications Trellis Then install latest Trellis software suite Format USB drive to FAT32 using a Windows PG Mac OSX 10 9 x and above has a feature called App Nap which regulates apps that it believes are not in use to consume less power Disable App Nap globally as detailed in Chapter 2 Close MATLAB and retry This occurs for mksort and or xippmex if MATLAB is open Use of this file currently requires the installation of Visual C Redistributable for Visual Studio 2012 This package may be found here and is available in x86 and x64 versions Run msconfig by typing lt window key gt R followed by msconfig Select the services tab and click Disable all click apply In the general tab select Normal Reboot computer Grapevine User Manual Document Version RO1550_08 Fa Appendix G Revision History Initial release Version 01 Version 02 Addition of standards specifications warnings warranty and RMA process Inclusion of size and weight for all Front Ends Heat Map feature added to Trellis Spike Grid application Safety Switch added to Trellis Stimulation Controller application Screenshot updates Inclusion of App Nap disable instructions for Mac OSX 10 9 x Mavericks users Version 03 Charge Balance parameter in Stimulation Controller Application changed to Phase Ratio
64. ed to the Grapevine NIP Note Front end cables should be removed from the port by squeezing and pulling the release tab on the connector only do not pull on the cable ERS Front Panel The front panel of the Grapevine NIP provides labeling for all of the connecters on the top and bottom panels of the unit There are a total of five indicator lights one for the unit s power status and four for the status of the front end ports power indictator neural interface processor front end port indictators Figure 1 4 Front panel of the Grapevine NIP Grapevine User Manual Document Version RO1550_08 10 ERS Power Indicator LED The power indicator LED will turn blue and then orange when the power supply is plugged into the Grapevine NIP indicating that the Grapevine NIP is in startup mode After a few seconds this light will turn green to indicate that the Grapevine NIP has initialized and is streaming data When the Grapevine NIP is not initialized the power indicator will be blue to show that the unit is in standby mode The light will only turn off when the power supply is unplugged from the unit Table 1 1 Power Indicator states Status Indicator Meaning Off No power The Grapevine NIP is not plugged in Blue Standby The Grapevine NIP is plugged in but not ready for recording Streaming The Grapevine NIP is sending data over the network for Green a aes visualization and capture Orange Initializing The Grapev
65. enable up to 24 additional analog inputs on a single cable Ripple supplies a standard 25 pin Micro D to Micro D cable contact Ripple for specialized cabling needs Additionally Ripple produces the Grapevine Analog I O Breakout Box that connects to the 25 pin Micro D port and houses 24 BNC terminals and a ground reference More detailed specifications for this connector including pinout information can be found in Appendix A The right side panel of the Grapevine Analog I O contains 4 SMA ports and a 25 pin Micro D connector for analog outputs A signal acquired by any FE can be reproduced on an analog output channel scalable up to 5 V See the Instruments gt Analog Outputs section of Chapter 3 for detailed information on analog output routing of continuous data streams Note Only one Analog I O Front End can be connected to an NIP The Scout Processor has a built in Analog I O and cannot have additional Analog I O Front Ends connected mounting hole SMA analog input ports 1 channel P4 per port N Micro D analog inputs 24 channels NS pz dnosb jndu LEFT Micro D connector P4 to grapevine NIP B SMA analog output ports 1 channel N Micro D analog outputs a 24 channels output group 1 24 ripple audio out 1 2 audio in 1 2 audio out 3 4 hole audio input 2 channels audio output 2 channels BOTTOM audio output 2 channels Figure 1 12 Complete
66. es module 33 255 properties module 34 54 properties KS S S S S Sg S S Sg Ki Figure Bb Modules of the Grapevine NIP and FEs Modules 1 and 2 shown here are for a single Nano2 Front End Nano2 Stim 3 amp 4 Micro 5 amp 6 EMG 7 amp 8 AlO 33 and DIO 34 ba ba e File Tools Help trellis D ger 7 E Instrument Network TR 4 Grapevine NIP 1 diesen gt MH Front Ends Xipp Property ID 0 Eaa gt WW Data Streams Xipp Property Type XippModuleDesc XIPP_FLAG_HIDDEN 0 I e XIPP_FLAG_INACTIVE 0 gt module 2 670 properties XIPP_FLAG_MUTABLE 0 gt module 3 324 properties XIPP_FLAG_NORESTORE 0 gt module 4 670 properties XIPP_FLAG_QUIET 0 gt module 5 324 properties XIPP_FLAG_RESET 0 gt module 6 670 properties eee gt module 7 90 properties State Running gt module 8 129 properties BEER gt module 33 255 properties Vendor ID 1 gt module 34 54 properties Module Type ID 0 Block Count 4 Total Property Count 33 Clock Domain Descriptor Property GUID 1 0 8 Module Size in Bytes 11224 Vendor Label Ripple Ee Diiia Interface Processor Software Version 1 6 0 rc25 Hardware Version R00244 02 Figure B 6 Module 0 Grapevine NIP information Grapevine User Manual Document Version RO1550_08 Di ERG File Feb Help Figure B 7 Modules 1 32 Front End amplifier information Figure B 8 Modules 33 and
67. es or adapters by touching the screw terminals of the component cable or by touching the metal shell of the component Users should ensure that the subject is properly grounded before connecting the Grapevine component to electrodes attached to the subject Product Cleaning Grapevine components should only be cleaned with lightly wetted cloths using water or alcohol Grapevine components should not be immersed in or sprayed with any liquids or gases Warranty Products provided by Ripple are warrantied to be free from defects in materials and manufacturing for a period of one year from the date of shipment This warranty is voided by abuse negligent handling or misapplication of the product or modifications to the product by persons not authorized by Ripple Any product that does not comply with this warranty shall be replaced or repaired at the option of Ripple Unless addressed in a separate agreement this constitutes the sole warranty provided by Ripple and there are no other warranties expressed or implied which extend beyond those described herein or to anyone other than the original purchaser including the implied warranties of merchantability and fitness for a particular purpose In no event shall Ripple be liable for any direct indirect incidental or consequential damages or for the infringement of any patent rights or third party rights due to the use of its products or consulting and design services System Calibration and Diagnosti
68. f 2 kQ and are produced at 10 kS s See the Instruments gt Digital Outputs section of Chapter 3 for detailed information on digital output event markers ERS Analog I O PN R00407 Size 105 mm x 60 mm x 14 mm LxWxD Weight 100g Analog Inputs The Grapevine Analog I O PN R00407 is capable of recording up to 28 analog inputs On the left side of the Grapevine Analog I O are 4 SMA connectors and a single 25 pin Micro D connector for input use Each SMA connector can receive a single channel of analog input connection whereas the Micro D connector can receive 24 channels of analog input The remaining pin provides a ground connection Details of the pinout can be found below in Figure A 5 The Grapevine Analog I O inputs are sampled at 1kS s and or 30 kS s and have an impedance of 10 MQ The ADC on the analog inputs has a 16 bit resolution and a range of 5V 0 15 mV bit Figure A 5 Pinout configuration of the Grapevine Analog I O Micro D connector Analog Outputs The Grapevine Analog I O is capable of producing up to 28 analog outputs Using the Trellis software input signals from any FE can be reproduced on the analog outputs On the right side of the Grapevine Analog I O there are 4 SMA connectors and a single 25 pin Micro D connector for output use Each SMA connector provides a single channel of analog output whereas the Micro D connector provides 24 channels of analog output The remaining pin is used for a common gro
69. ferential biopotential recordings are first filtered in hardware in the Grapevine Surf D through a high pass filter with a 0 3 Hz cutoff and low pass filter with a 7 5 kHz cutoff The ADC of the Surf D FE has 16 bit resolution with 0 25 uV bit and an input range of 6 5 mV Each channel of the Surf D is sampled at 30 kS s and has an input impedance of 250 MQ in parallel with 20 pF with input referred noise of less than 2 1 uV RMS Note that G indicates the ground sockets and N C indicates that the socket is not used Ei Document Version RO1550_08 Appendix A Technical Information om Se Appendix B System Information This appendix describes m detail how to access the hardware information for the Grapevine NIP and Front Ends as well as system information for the Trellis software suite This information will be useful if problems arise and there is a need to contact support engineers at Ripple Current Trellis software for a desired operating system platform can be downloaded from the Ripple website The firmware for the NIP and Front Ends is programmed at the time of production however the NIP firmware can be updated When new versions of the NIP firmware are completed by the developers a support engineer will be in contact and details on how to update the Grapevine NIP will be provided Trellis Software The Trellis software information can be found by running Trellis and clicking on the Trellis icon in the upper left as shown in Figu
70. fety switch Grapevine User Manual Document Version R0O1550_08 ES ERS File Save File Save provides an interface to specify which data streams will be recorded as well as to start pause and stop individual recordings For each recording the file name and comments can be specified The performance of the data acquisition system is strongly dependent on the speed of the file media being used data should only be recorded to a local hard drive If data files are to be stored on a network drive it is recommended that the data files be moved after recording is finished Note The NEV Specifications document details how the data is stored in the saved Trellis files The document is provided with the Trellis software suite and is also available on the Ripple downloads webpage Data is stored according to channel IDs which are numbered 1 5120 for Front End recording channels 5121 10240 for Front End stimulation channels and 10241 10270 for analog input channels 4 SMA 24 grouped and 2 audio inputs Digital input events are stored in the nev file under packet ID 0 Recording channels of Front Ends connected to port A of the first NIP are numbered 1 128 then 129 256 for port B etc Recording Front End channel IDs are numbered 1 5120 to allow for recording from multiple NIPs by one computer running Trellis which will be enabled in a future software release A sample saved data set that includes spikes LFP Hi Res raw stim digital input
71. grapevine User Manual Salt Lake City UT USA This document was as far as possible accurate at the time of release However subsequent changes may have been made to the software and hardware Ripple reserves the right to alter specifications and details as required Late breaking information may be supplied separately for completeness Trademarks of Ripple ripple and grapevine are registered trademarks of Ripple The names of specific devices such as Grapevine NIP are trademarks of Ripple Trellis and Scout are trademarks of Ripple Other Trademarks Bi Lobe is a registered trademark of Omnetics Connector Corporation Apple Mac Macintosh and OS X are registered trademarks of Apple Computer Inc Windows and Windows 7 are either registered trademarks or trademarks of Microsoft Corporation Linux is a registered trademark of Linus Torvalds LEMO is a registered trademark of LEMO USA Inc MATLAB is a registered trademark of The MathWorks Inc Intel is a registered trademark of Intel Corporation NETGEAR is a registered trademark of NETGEAR Inc All other trademarks are the property of their respective owners Product Grapevine Neural Interface System For use with Trellis software suite version 1 6 1 Copyright December 2015 Ripple LLC 2056 South 1100 East Salt Lake City UT 84106 USA All rights reserved No part of this document may be reproduced by any means without the prior written permission of Ri
72. h signal The running estimate of the mean squared values has a first order time constant of approximately 2 seconds and settles in 4 to 6 seconds As a direct RMS estimate it can be biased by spikes and strong transients in the signal If a channel has dual thresholds set the threshold is changed to a single positive or negative value specified by the sign of the multiplier o Multiple of RMS Median default Computes the threshold as the specified multiple of the running median estimate of the spike bandwidth signal or Median Absolute Deviation MAD The MAD calculation algorithm settles in approximately 5 to 10 seconds and it is scaled by 1 482 so that the result approximates the RMS multiplier method for Gaussian noise If a channel has dual thresholds set the threshold is changed to a single positive or negative value specified by the sign of the multiplier e Digital I O Options This panel enables two features for the Digital I O Front End o Loopback UDP triggered digital output Enabling this checkbox configures the NIP to echo back onto the instrument network all Digital I O outputs that are emitted in response to requests by other entities on the instrument network This option must be enabled to run the network latency test See Appendix D for more information on controlling the Grapevine NIP with custom software O Capture digital input on parallel bit change By default the state of the 16 bit parallel port on the Dig
73. hange Off e Analog Outputs o Enable Off o Source Channel Off o Signal Type Off e Digital Outputs o Enable Off o Source Channel Off o Signal Type Off Grapevine User Manual Document Version RO1550_08 Se p lt SS e Fast Settle Triggers o Digital Inputs None When High 0 5 ms o Stim Any Front End 0 5 ms o Auto Off e DSP Filter Settings o Hi Res Filter 0 3 500 Hz Micro Micro HV Micro Stim Nano EMG Surf S 0 1 500 Hz Nano2 Hi Res Notch Filter Off o LFP Filter 0 3 250 Hz Micro Micro HV Micro Stim Nano Surf S 1 250 Hz Nano2 LFP Notch Filter Off o Spike Filter 250 7500 Hz Micro Micro HV Micro Stim Nano Nano2 O O e Front End Hardware Nano2 Nano2 HV and Nano2 Stim only o Hoh Dass Filter 0 1 Hz Nano2 only o Recording Resolution 0 25 uV bit 8 mV range Trellis Software Suite Below are the default startup states that are specific to the Trellis Applications Some of the settings below are dependent on which Front Ends are connected to the system Some of the Trellis settings can be considered sticky and will be set at startup based on what was used during the last Trellis session e Trellis Main Instruments o Analog Outputs Tracking Off o Digital Outputs Tracking Off e Application Auto Restore The Raster Spike Grid Spike Scope and Impedance Analyzer Applications have the ability to auto restore all their settings from the
74. hase ratio of 1 2 means that the second phase will have half the amplitude of the leading phase but the duration will be twice as long Selecting the Open phase ratio allows the user to have direct control over the Phase 2 parameters described below The default charge balancing is 1 1 Because some Stim FEs have a large stimulation amplitude step size it 1s not always mathematically possible to achieve the specified ratio between phase parameters When this is the case the closest valid phase settings are used Note The Stim Front Ends are capacitively coupled which ensures total charge balance over time for all stimulation pulses e Phase 2 These two controls specify the parameters of the second phase of the stimulation waveform If Phase Ratio is set to anything besides Open these two controls will be disabled and automatically updated to reflect the desired phase ratio As with Phase 1 parameters the default amplitude is three times the stimulation step size specific to the FE and the default duration is 100 us The limits and resolution are the same as for Phase 1 e IP Interval This control specifies the interphase interval in microseconds between Phase 1 and Phase 2 of the stimulation waveform The maximum duration is 50 ms There is technically no minimum duration for the IP Interval however due to constraints of the stimulation hardware the minimum allowable IP Interval is determined such that the IP Interval Phas
75. he system is a Neural Interface Processor also referred to as the Grapevine NIP The Grapevine NIP interfaces with up to sixteen Front Ends FEs which filter amplify and digitize signals from both high impedance and low impedance electrodes Example electrodes include microelectrodes bare wire electrodes surface electrodes etc The Grapevine NIP also interfaces with FEs that provide analog and digital I O capabilities The Grapevine NIP can only be used with Grapevine FEs manufactured by Ripple The Grapevine NIP connects to a computer via a gigabit Ethernet connection The Trellis software suite provides an interface for controlling instrument settings data recording stimulation and visualization of acquired data The generalized Grapevine system workflow is shown below Subject with e i a n sya Grapevine Neural Ethernet Trellis Software Suite Implanted Grapevine Front Ends Front End eiaa Cabi e e E Cables nterface Processor e on Computer 0 3 Hz High Pass 7 5 kHz Low Pass 3rd Order BW Raw Data Stream Stream ing Display of 30 kHz Sampling Selected Data Streams Spike Grid Spike Data Streams Spike Scope Stream and Window of Seected Spike Data Stream Including Unit Sorting Impedance Measure Impedance of Electrodes File Save Save Data Streams to Hard Drive Instrument Configuration Configure Data Streams and Filters Hi Res Data Stream Proprietary 2 kHz Sampling XIPP UDP 24 bit Floating Point Precision
76. icro HV and Micro Stim have high input impedance they are suitable for connection to microelectrode arrays and other implanted electrodes Acquired signals are first filtered using a high pass filter with a 0 3 Hz cutoff and low pass filter with a 7 5 kHz cutoff The ADC has 16 bit resolution with 0 2 uV bit and an input range of 6 mV Each channel is sampled at 30 kS s and has an input impedance of 450 MQ in parallel with 15 pF capacitance with input referred noise of less than 2 1 uV RMS All recording electrode channels are referenced to a single reference electrode with selectable configurations see Table 1 4 Note that G in the pinout configuration indicates the ground connections and RI and R2 indicate the Reference and Reference 2 connections The maximum safe input voltage is 5 V The Micro can measure electrode impedances between 1 kQ and 1 MQ The Micro HV has additional circuitry for high voltage protection in applications where nearby stimulation may occur The Micro Stim FE is capable of delivering current controlled stimulation on up to 32 independent electrodes with a range of 1 mA using 127 discrete steps of 7 5 uA and a compliance voltage of 9 V All stimulation is in reference to the ground connection Stimulation and recording can be done simultaneously with the Micro Stim FE E Document Version RO1550_08 Appendix A Technical Information Surf S PN R00318 Size 51 5 mm x 36 5 mm x 4 75 mm LxWxD Weight 12 5
77. in the File text field The directory for saving files can be selected by clicking on the folder button to the right of the File text field File extensions are automatically set depending on the type of data recorded e Auto Increment When this box is checked filenames will include numbers at the end of their names e g c datafileQ002 nev The number will be automatically incremented as data files are collected and the number of initial leading zeros will be preserved The starting number can be changed by entering a new value in the numerical field Grapevine User Manual Document Version RO1550_08 eal ERS EI Document Version RO1I550_08 Chapter 3 Trellis Software Suite Comments This field contains a comment up to 200 characters that is inserted in the header region of the data files Multiple lines can be entered with Ctrl Enter However only eight lines of text will be saved Stop After timed recording With the Stop After box checked specify an amount of time in seconds minutes hours etc for the Grapevine to record data Once the time has expired the recording will stop automatically Segment Recording Every When this box is checked files will be saved with the maximum data size or time length selected When the specified data size or time length is reached for the current data file additional data will be saved to a new data file Segmented data files will use an auto segment suffix date or number which can
78. ine is powered on and initializing Front End Port Indicators The front end port indicator LEDs will turn green when a front end cable attached to a functioning FE is connected to a front end port Note All desired FEs must be connected to front end ports before the NIP is powered on Table 1 2 Front end port indicator status Status Indicator Meaning No connection No Grapevine front end cable is connected to the port Ott or the connected front end cable is not properly configured Connected The Grapevine front end cable is connected to the port Saas and properly configured Orange Error Red Fault Generally this occurs when a power supply has detected excessive power draw If this occurs shutdown the system for safety DI Document Version RO1550_08 Chapter 1 Hardware Overview ES Back Panel The back panel of the Grapevine NIP is where the speaker and cooling fan are located Note The speaker is not currently used by the Grapevine NIP Note The VGA plate next to the speaker should remain attached at all times The VGA port beneath is for Ripple development and testing purposes only fT L a ioe ONIMMNMIMNIMMNIANIAMNI fan housing speaker UNO Figure 1 5 Back panel of the Grapevine NIP Fan Housing To ensure the continuous operation of the Grapevine NIP the housing of the Grapevine NIP is designed to act as a heat sink The enclosed cooling fan provides sufficient airflow to maintain the elect
79. is can be configured to periodically autosave the instrument configuration click on the more link to access this option This panel also displays a list of previously saved configuration files A saved configuration can be applied to an instrument by selecting the configuration from the list and clicking the Load button If the configuration is loaded successfully the text load successful will appear to the right of the item in the list Only saved configurations that match the hardware currently connected to the NIP can be loaded and inappropriate configurations will be grayed out Front End information about each configuration file can be viewed by hovering the cursor over the file name Grapevine User Manual Document Version RO1550_08 30 p lt SW e Global Auto Thresholding This panel contains options for setting system wide spike thresholds The first dropdown specifies how the threshold is determined The second dropdown specifies how the threshold is applied Following is a description of the program behavior for various threshold algorithms when the Single only option is selected o Absolute Level uV Sets the threshold to the specified value If a channel has dual thresholds set the threshold is changed to a single positive or negative value based on the sign of the specified value o Multiple of RMS Computes the threshold as the specified multiple of the running RMS estimate of the spike bandwidt
80. ital I O Front End is only captured when either the Strobe A or Strobe B port is set high This checkbox enables capture of the 16 bit parallel port state when there is a bit change on any of the parallel port lines e Analog Outputs This panel enables routing of selected neural data Raw LFP Hi Res or Spike Filtered to the Analog I O output ports The analog output gain is 1000x for the Raw data stream 1 mV input 1 V output 5000x for the LFP and Spike Filtered data streams 1 mV input 5 V output and 250x for the Hi Res data stream allows for full range of EMG Front End Note The analog audio output gain is 500x for Raw data streams 1 mV input 0 5 V output and 2500x for the LFP Hi Res and Spike Filtered data streams ImV input 2 5 V output The output data is digitized at 30 kS s and there is a fixed delay of 5 ms for 30 kS s Raw data 6 ms for 2 kS s Hi Res data and 7 5 ms for 1 kS s LFP data When tracking is enabled the Analog I O output port will adjust and follow the active channel that has focus Note Only Spike Filtered data is routed to the selected Analog I O output port when tracking is enabled e Digital Outputs This panel enables the Digital I O Front End output ports SMA 1 4 and parallel 1 16 to emit a 1 7 ms LVTTL pulse when a spike event occurs on a selected neural channel When tracking is enabled the port will adjust and follow the active channel that has focus The exact latency from a spike event to the
81. ized within each FE The information passed from the FEs to the NIP is in digital form making the Grapevine system very resistant to environmental noise Data sent from the Grapevine system to a computer contains channel data sampled at a maximum of 30 kS s 2 kS s maximum for data from the Grapevine EMG Front End Data is transferred between the Grapevine NIP and the computer using User Datagram Protocol UDP UDP data is encoded using the eXtensible Instrument Processing Protocol XIPP format a propriety protocol developed at Ripple Decoding the XIPP packets can be done using headers provided by Ripple These headers enable users to create custom applications that can access and process data from the Grapevine NIP with low latency Please contact Ripple if this is necessary for an application Supported Front Ends Nano2 Nano2 HV Nano2 Stim PN RO2001 Nano2 RO2002 Nano2 HV RO2003 Nano2 Stim Size 19 mm x 15 mm x 3 5 mm LxWxD Weight 2 0 g Nano2 3 5g Nano2 HV and Nano2 Stim The Grapevine Nano2 FEs use a 4 guide post 36 position Omnetics nanostrip connector PN A79023 001 mates with PN A79022 001 A79024 001 or A79026 001 connectors to interface with electrode platforms using a pin configuration shown in Figure A 1 Because the Grapevine Nano2 FEs have high input impedance they are suitable for connection to microelectrode arrays and other implanted electrodes Acquired biopotential signals are first filtered using a
82. ke Grid The Spike Grid contains a variety of controls to customize the view A graphic and description of each control is explained below Opens a dialog box for selecting and loading an electrode map file see following description Switch between Spike Grid display shown above and Heat Map display see following description Load Map Spike Grid Heat Map Scale 200uv Set voltage scale on the selected displays M Decreases the voltage scale on the selected displays Wel Increases the voltage scale on the selected displays 50 Spikes Sets the number of most recent spikes overlaid in display Clear Manually clears spikes in selected displays E Document Version RO1I550_08 Chapter 3 Trellis Software Suite ES Heat Map Display The Spike Grid application contains a Heat Map display where the frequency of spike events is shown for each Front End channel The frequency is shown in a color coded format where the warmer end of the color spectrum is used for more frequently active channels The frequency statistics for all channels are displayed on the right side of the top control bar and can be shown for individual channels by selecting a channel in the grid By default all FE channels connected through the Grapevine NIP are displayed Double clicking on a channel display launches the Spike Scope application for that channel see next section for details on the Spike Scope Note If the width of the Heat Map Display i
83. last session through the Tools gt Options menu in the top menu bar e Raster If restore is not enabled o Data Streams LFP On Hi Res On Raw On Spike On Spike Preview Off Stim On Analog 1k On Analog 30k On Digital Events On o Colors Off o Signals Grouped by Data Stream Ei Document Version RO1550_08 Appendix C Hardware amp Software Configuration ES e Spike Grid If restore is not enabled o Heat Map Off o Scale 200 uV o Hold 50 Spikes o Electrode Map Unloaded e Stimulation Controller o When the Stimulation Controller App is relaunched the last electrode s selected during the previous sessions will again be selected and active in the Waveform Period and Train displays e File Save defaults o Data Streams based on which Data Streams are enabled in Instruments LFP On Hi Res On Raw Off Spike On Stim On Analog 1k On Analog 30k Off Digital Events On o Digital Triggering Off o File Save Name Location lt homeDir gt Trellis dataFiles lt date gt datafile e File Save sticky settings loaded based on last Trellis session o Data Streams and Individual Channels Auto Increment Stop After Save Instrument Configuration Segment Recording Every Play Tone on Start Stop Enable Remote Control O O OOOO Trellis Configuration File The Trellis Configuration File contains saved settings for both the Grapevine System h
84. lis software suite This can be accomplished either through a direct connection to a low latency 1 Gb network interface card or through a low latency 1 Gb network switch Ripple recommends that Windows users contact the computer manufacturer Dell Lenovo etc to determine which network interface card or Ethernet adapter is best suited for their system The NETGEAR GS105 network switch is recommended by Ripple for multiple computer configurations Direct Connection to Computer Use a gigabit Ethernet cable to connect the Ethernet port of the Grapevine NIP labeled eth or Ethernet to a dedicated Network Interface Card NIC in the host computer Use the following sections to configure the computer s NIC for communication with the Grapevine NIP The Grapevine NIP has a static IP address of 192 168 42 1 The operating system s network configuration needs to be set up on the same subnet as the Grapevine NIP to enable data communication 192 168 42 x where x is in the subnet range of 129 254 Multiple Computer Connections to the Processor Multiple computers can be connected to a single processor through the use of a gigabit Ethernet switch such as the NETGEAR GS105 Each additional PC must also have an Ethernet connection that belongs to the instrument subnet The IP addresses for these computers should be incrementally numbered 192 168 42 129 192 168 42 130 etc up to 192 168 42 254 neural interface processor l i ripple
85. m and Trellis software suite This appendix addresses issues that may not be consistent across all customers Consistent replicable problems will be addressed by Ripple engineers This appendix will be updated with problems found by customers and solutions determined by Ripple All solutions have been tested as much as possible by Ripple Product Trellis 1 4 0 Windows 7 Trellis 1 4 0 Mac OSX 10 8 4 NIP update 1 4 0 Trellis 1 4 0 Mac OSX 10 9 x Mavericks and newer Trellis 1 4 1 Windows 7 Trellis 1 4 3 Windows 7 Trellis 1 4 3 Windows 7 Table D 1 Grapevine system troubleshooting solutions NIP not sending data packets Network Status stuck at searching Installation failed to overwrite a previously installed Trellis software suite NIP failed to update because USB drive is improperly formatted from Mac machine Network packet drops begin to occur when any Trellis application is opened and the Trellis main application is moved to the background Installation fails because The installer has insufficient privileges to access this directory C Program Files Ripple Trellis Tools mksort docs Error importing files into Plexon Offline Sorter error unable to open C Program Files x86 Ripple Trellis Tools thirdparty nsNEV Library x64 nsNE V Library dll Data packet loss even with properly configured low latency gigabit network adapter Solution Disable Microsoft S
86. mulation Controller single electrode stimulation pattern On the left of the Stimulation Controller window are several information and control panels that can be expanded and minimized by selecting the or next to each panel label A detailed description of the panels follows Refer to Figure 3 21 e Stimulation Hardware This panel displays a list of all available stimulation channels In parentheses next to each electrode is a label indicating the number of different stimulation patterns that have been created for that electrode When a channel is selected its current stimulation pattern is displayed in the Stim Parameters control panel If no patterns have been defined for a selected channel a default pattern will be created indicated by a in the channel list as shown in Figure 3 21 An electrode map file can be used for electrode labeling by switching to Electrode View and loading the desired map file Ap Document Version RO1I550_08 Chapter 3 Trellis Software Suite ES e Hardware Description The technical specifications of the FE will be displayed here when an electrode is selected in the Stimulation Hardware panel including the stimulation current step size shown as 7 5 uA for the Micro Stim FE in Figure 3 21 For Micro Stim FEs this is the discrete amplitude step size that is programmed in the FE at the time of production The step size value must be entered when the Micro Stim is first loaded in the Stimulation
87. n the installed directory Ripple Trellis Tools and can be tested with a sample saved data set that includes spikes LFP Hi Res raw stim digital input and analog input data may be downloaded here Neuroshare Inside the Tools directory is a directory labeled neuroshare containing a set of functions based on the open source Neuroshare API These functions can be used to extract digital and analog data that are acquired from any of the Front Ends along with additional information such as event timing hardware information and channel configuration There are a number of example scripts in the neuroshare examples directory that will demonstrate how to properly extract and plot common data types such as spike waveforms spike histograms and continuous analog data Wisteria The Wisteria Offline Data Viewer displays data that is saved by the Trellis software in a manner similar to the Raster Application and can be found in the Tools directory Tools wisteria The viewer can be run by including the wisteria and neuroshare directories in the path or by setting the working directory to be Tools wisteria and then running wisteria from the command window To load a data file select File gt Open and then navigate to the saved data file The toolbar buttons in Wisteria control which channels are viewed and what the plot scaling is similar to the buttons found in the Raster Application of Trellis Ad
88. nd of the present set of patterns for the channel You can edit any pattern in the Stim Score by selecting it indicated by an orange box surrounding the pattern in the Score Note When patterns are added to the score a pointer to the original pattern is created 1 e patterns are not replicated when added to the score Thus if a pattern is edited in the Stim Parameters panel that is used in the score it will change the pattern in the stimulation score An individual stimulation pattern can be removed from the stimulation score by selecting it and clicking on the button in the panel tool bar Channels and all of their patterns can be removed from the stim score by clicking on the channel label and then clicking the button A single pattern for multiple electrodes can be selected by clicking on a pattern and then dragging the mouse pointer up or down The time scale x axis of the stim score can be altered by positioning the cursor over the display and using the buttons that appear or by scrolling the mouse wheel while holding down the Shift button Multi Channel Stim elec B 1 01 Loop i elec B 1 07 DOSI 06 eo Oe EE a Wel Stim Channels a zl gt Figure 3 25 Trellis Applications Stimulation Controller Multi Channel Stim Display 0 00 sec Note Any warnings or errors will be displayed on the top bar of the Multi Channel Stim Panel as shown in Figure 3 26 If the duration
89. nnunnnnunnnnunnnnnnnnnnnnnnnnnnn nnmnnn 8 Neural Interlace Processor NIP E 8 Frontend Interlace Cables caserne E 13 gereent 13 EE 13 EE 13 SUN ee 13 CM GAR seer itor ER PRION ERE Ee ENDO EES EEN RE Na EO EEO DE 13 DIG IOI O arene EE tne sn ia etree ea canals ear aan ne ele O OOE 17 ANAO GA EE 19 SCOUT OCCSS O E 21 CHAPTER 2 INSTALLATION AND SETUP eege eege 22 Connecting the Hardt eege EE 22 Direct Connection TO Computer sixes dwn aan ine A 22 Multiple Computer Connections to the Processor ssssesssssresressrssrssrenrensnesneonrennnsnnsnnenrennnnnes 22 Windows 7 Network Configuration csssssssesesssssessasscsessessecsassessarecsasseseacsassesesressassestarseses 23 Mac OS X Network Conficurato E 25 Linux Network Conn SUA ee EE 26 CHAPTER 3 RELLIS SOFTWARE SUITE aiaa rele eae e aaa aaa ea aaa cee ae 27 Using Trelis Steeg dee Dan eaaa aaae 27 SEELEN eege 28 ek e ee et EE 33 E 33 yO LRE gh E 35 Heat Map DIS Eegeregie 36 PT Oe MIDS eege 37 SPIKES COPE este sect sceae techs etter a a Ouest ested a a a a a 38 heute re 42 engl WU Fei pls eege 44 e TE 51 APPENDIX A TECHNICAL INFORMATION uvssssseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeesneseeeeseeenenseeeeeeesenneneenennnes 54 Supported Front ENS sciora anessan aaiae anaana araa Eau daraa raaraa isaka kaaa 54 Nano2 Nano HVNapno itrktm 54 NO EE 56 MicroZ MicroZ HV MICrO2 4S UM EE 56 Micro Micro HV and Micro tS tin EE 57 AN WEE 58 Eeer 58 Buste
90. of stimulation for a pattern is not an integer multiple of the stimulation period 1 frequency the train will have an empty partial period at the end of the pattern noted by a red box in the Stim Score as shown in Figure 3 26 Patterns that are less than 10 ms in duration cannot be executed in line with other patterns in the multi channel stim score Patterns that are less than 25 ms in duration are not recommended for more than 128 channels of stimulation Multi Channel Stim The multi channel stim score has warnings details Figure 3 26 Trellis Applications Stimulation Controller Multi Channel Stim Display Warnings amp Errors 49 Document Version R01550_08 Chapter 3 Trellis Software Suite Once the stimulation parameters have been set for one or more electrodes the stimulation can be executed by clicking on the play button to the right of the Stim Parameters panel display or the play button to the right of the Multi Channel Stim display Note When executing stimulation scores a Stimulation Safety Switch similar to Figure 3 28 will appear in the top right of your computer display This can be used to safely stop all stimulation if an adverse event occurs This runs as a separate application to the Stimulation Controller app so that stimulation can be stopped immediately if necessary The Stim Parameters panel play button shown in the top of Figure 3 27 below will execute the current pattern on the channels selected in
91. orm data for low frequency signals such as LFP ECoG EEG and EMG The LFP signals are sampled at 1 kS s per channel and are digitally filtered using a low pass IIR filter of 250 Hz with 4 order Butterworth characteristics o Raw Signal streams contain continuous waveform data with the entire bandwidth of the FE amplifier The raw signal is sampled at 30 kS s per channel o Spike streams contain neural events that cross the user defined threshold for a channel Each spike data packet consists of 52 samples captured at 30 kS s 1 7 ms and are digitally filtered with a high pass filter of 250 Hz default Spikes are aligned to threshold crossing with 15 pre threshold samples included in the waveform Note Spike events occurring faster than every 1 7 ms will not be completely captured as Spike event data packets do not overlap o Stim streams contain the voltage waveforms supplied by the Micro Stim Front End during stimulation captured at 30 kS s in 52 sample data packets Note The Hi Res data stream is the only option available for the EMG Front End e Saved Configurations This panel provides the ability to save and load instrument configurations Each configuration file saves the settings for data stream initialization Front End outputs fast settle triggers filter specifications and per electrode data streams for file saving The NIP configuration may be saved at any point by clicking the Save Current button Alternatively Trell
92. orm display in Figure 3 24 the Period display shows a single period of stimulation for the present stimulation pattern The Offset time and the entire duration of stimulation are depicted The time scale can be changed by positioning the cursor over the display and using the buttons that appear or by scrolling the mouse wheel while holding down the Shift button Grapevine User Manual Document Version R0O1550_08 48 ERS Train Display Shown below the Period display in Figure 3 24 the Train display shows all stimulation pulses for the present stimulation pattern The onset of each stimulation pulse is noted by a tick mark in the Train display The time scale can be changed by positioning the cursor over the display and using the buttons that appear or by scrolling the mouse wheel while holding down the Shift button Multi Channel Stim This panel provides a means for constructing stimulation scores involving one or more electrodes and one or more patterns per electrode as shown in Figure 3 25 below By default this panel is collapsed and can be expanded by clicking the symbol next to Multi Channel Stim on the bottom left of the Stim Controller app A stimulation pattern for an electrode can be added to the Score by selecting the pattern in the Preview section of the Stim Parameters panel and then clicking on the button on the top of the Multi Channel Stim panel The pattern will be added to the Stim Score at the e
93. parameters for one or more electrodes as described in the following sections Note Micro2 Stim and Nano2 Stim FEs support calibration of stimulation output which is described in the Instruments section of Chapter 3 Note If Trellis is closed from the main application before stimulation completes or if there are new stimulation parameters set there may be an additional window open requiring user attention to stop stimulation or save stimulation parameters Note There is a known issue with the Micro2 Stim and Nano2 Stim FEs where simultaneous stimulation on multiple electrodes causes distortion in the stimulation output Please see the discussion on this topic for these Front Ends in Appendix A D emgeet Stimulation Controller Be File Tools Help Stimulation Hardware Stim Parameters Hardware View _ Electrode View 4 Grapevine NIP XippID 1 4 Port A uA usec Charge Balance _ Amp 2 Amp 1 4 PortB stim front end 4 Port C 4 PortD Hardware Description or Gees 1 electrode selected for stim P Stim Configurations P I Current File untitled stm v Stim Patterns v Stim Score Save Saved Files Browse seh Dir C Users Mitch Multi Channel Stim Figure 3 19 Trellis Applications Stimulation Controller Grapevine User Manual Document Version RO1550_08 PA ERS a d You must specify the amplitude step size for a stim front end befor
94. ple electrodes can be adjusted by selecting them from the Preview section When multiple previews are selected controls corresponding to parameters that are not the same across the selected patterns will be blank In the simplest use case each channel will have only one pattern however it is possible to define multiple patterns for each channel To create a new pattern for the present channel or if multiple channels are selected in the preview column click the New button in the panel toolbar To change the present pattern for a channel select only that channel and then use the lt gt buttons in the panel title bar to navigate through the set of defined patterns The time and amplitude scales of the different stimulation pattern displays can be increased or decreased by positioning the cursor over the display and using the mouse wheel Shift or mouse wheel Alt respectively A stimulation parameter can be changed either by entering a numerical value in the corresponding field or by selecting the field noted by an orange box around it and then using the parameter dial pictured below the parameter fields in Figure 3 21 and close up in Figure 3 22 below The dial can be rotated by clicking on it and dragging the mouse or by positioning the cursor over the dial and rotating the mouse wheel hold down Shift for a larger step size The increment for changes in parameter value can be set by selecting one of the values below the di
95. pple Website www rippleneuro com Technical Support support rppl com Document Version RO1550_08 Human Use Considerations The Grapevine Neural Interface System described herein with exceptions noted has been designed for clinical use with humans The system has been used with humans through Institutional Review Board approval at various universities and institutions Ripple has tested all components of the Grapevine Neural Interface System for isolation and leakage current per IEC 60601 1 standards Components of the system that are not intended for human use are specifically noted throughout this manual Standard Symbols in Use Danger of IEC 60101 0102 Electrostatic TEC 60417 5031 Direct Current Discharge ESD Dangerous IEC 60417 5032 Alternating IEC 60417 5036 Voltage Current Type of CF IEC 60417 5021 Equipotentiality IEC 60417 5335 Applie d Part Connector Warning IEC 60417 5008 OFF power EN 980 Serial Number IEC 60417 0007 ON power Specifications 110 VAC at 60 Hz or 240 VAC at 50 Hz 8 A max load Serviceable Fuses 5 x 20 mm 250 V 2 A Slow Blow Scout processor only e EE GE EU Directive 2004 108 EC EMC and Directive 2006 95 EC LVD P EN 61326 1 2013 EMC EN 55011 EMC EN 61010 1 2010 LVD Type of Protection Class I Continuous Ordinary Equipment not fluid resistant IP20 10 C to 40 C 5 to 95 R H non condensing 20 C to 50 C 5 to 100 R H non condensing Degree of P
96. r writing custom programs to control the Grapevine NIP are included with the Trellis software suite in the Tools xippmin directory These headers may be useful for applications requiring low latency access to data acquired by the Grapevine NIP There are also some example programs in the xippmin directory for triggering Trellis and reading the data streams produced by the grapevine NIP Third Party Software Compatibility Ripple provides a Neuroshare dynamic link library nsNEVLibrary dll for use with third party software to import files saved by Trellis The library file is included in the Trellis software suite in both 32 bit x86 and 64 bit x64 versions and can be found in the Trellis Tools thirdparty directory Note Use of these files currently requires the installation of Visual C Redistributable for Visual Studio 2012 This package may be found here and is available in x86 and x64 versions Nex Technologies NeuroExplorer The NeuroExplorer program must first be configured by going to View gt Data Import Options gt Blackrock Microsystems Files selecting Use Neuroshare DLL and pointing the Neuroshare DLL Path to the correct Trellis Tools thirdparty nsNEV Library xX X nsNEV Library dll path Files can then be imported into NeuroExplorer as Blackrock Microsystems files Note Use of these DLL files currently requires the installation of Visual C Redistributable for Visual Studio 2012 This
97. re B 1 below This will then open a new window containing information about the software including the build number in bold at the top as well as the build date and source revisions as can be seen below in Figure B 2 File Jools Help trellis a PG Instrument Network a H Grapevine NIP 1 a Ru Front Ends A 1 nano RO0894 0004 v3 7 B 1 stim RO1289 0005 v2 1 C 1 micro RO0249 0112 v5 2 C 2 EMG RO 7008 0001 v2 1 D 1 digital IO ROO591 0027 v3 1 Figure B 1 Accessing the Trellis software suite information Trellis 1 4 0 274 Based on Trellis 1 4 0 274 32 bit Built on 2014 03 25 at 09 38 58 Source revisions Trellis XIPP 1684 ver 0 9 Copyright 2010 2013 Ripple All rights reserved The program is provided AS IS with NO WARRANTY OF ANY KIND INCLUDING THE WARANTY OF DESIGN MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE Figure B 2 Trellis software suite information Grapevine User Manual Document Version RO1550_08 62 ERS Grapevine Processors and Front Ends The hardware information for the Grapevine NIP and Front Ends can be found by using Trellis Make sure all FEs are plugged into the NIP before turning on the NIP power Run the Trellis software and if everything is configured correctly the display screen should look similar to Figure 3 3 and Figure B 3 below showing the Instrument Network and the attached Grapevine NIP To access the properties for the NIP right click on
98. ronics performing at standard operating temperatures Note The fan may change speeds to manage the system temperature The vents on the top and bottom of the Grapevine NIP allow air to flow and must not be obstructed by dust or other devices as this may impede ventilation and cooling Any obstruction of airflow may degrade the performance of the Grapevine NIP or even permanently damage the unit Grapevine User Manual Document Version R0O1550_08 bes ERS 13 Document Version RO1550_08 Chapter 1 Hardware Overview Front End Interface Cables The front end cables included with the Grapevine System should be handled with care Damage can occur if users pull on the gray insulation wrapping Cables should only be disconnected from Grapevine Processors and Front Ends using the following instructions To disconnect the cable from a Grapevine Processor pinch the LEMO connector and pull the connector gently from the processor To disconnect the BiLobe connector cable from a Front End Micro FEs Nano v1 EMG or Surf S unscrew the two bolts from the Omnetics BiLobe connector When both bolts are fully free the connector will come free from the Front End without any effort The Grapevine Nano2 Front Ends use a circular Omnetics connector that mates and disconnects with minimal force Front Ends There are a variety of Grapevine Front Ends FEs that connect the Grapevine NIP to electrodes as well as analog and digital signals The electrode re
99. rotection Type CF Applied Part ii Document Version R01550_08 Preface Warnings e The Grapevine system should only be used with components provided by Ripple or approved by Ripple engineers e Do not ingest any components of the Grapevine system e Only connect the Grapevine system to properly tested and grounded AC outlets Do not connect the Grapevine system components to an outlet controlled by a wall switch e Do not use the Grapevine system in the presence of flammable gases Keep all components of the Grapevine system away from liquids Contact with water can lead to electric shock e The Grapevine system is not sterile It may be used in conjunction with sterile devices but should be kept outside sterile environments e The Grapevine system may be used with third party components approved by Ripple engineers Follow all manufacturer recommendations for third party components as improper use can compromise the safety of the Grapevine system components Electrostatic Discharge Instructions Grapevine system components that are sensitive to electrostatic discharge are marked with the ESD symbol For these Grapevine components users should not directly touch any internal pins and should adhere to the following precautions when connecting electrodes or adapters to these components Users should always ground themselves before handling these Grapevine components Users should then ground the Grapevine component before connecting to electrod
100. s too narrow the electrode labels and some items of the top control bar will not be visible To view the labels and additional controls drag the Heat Map Display wider Eee SS eee file Jooks Help 4 Map File mapFileTemplate_ICS32_UEAmap Charge Geer Spike Grid Heat Map Refresh Every 500 msec Color Mapping 10 Hz QR D te Spike statistics 1 electrode mean 13 2Hz USEAL4ec001 EA 1 decd02 ISEA L eec003 ISEA 1 de0004 ISEA L elec005 USEA 1 decode IKEA 1 ele Figure 3 10 Trellis Applications Spike Grid Heat Map display The Heat Map display contains a variety of controls to customize the view A graphic and description of each control is explained below Opens a dialog box for selecting and loading an electrode Load Map map file see following description Spike Grid II Heat Map Switch between Spike Grid display and Heat Map display Refresh Every 500 msec Set the rate at which the display refreshes Set the frequency bounds for the Heat Map display The Color Mapping 1 0 Re values to the left and right of the color wheel are the minimum and maximum frequencies to be displayed Grapevine User Manual Document Version RO1550_08 Ea ERS Electrode Maps Map files enable the assignment of array and electrode labels to FE channels and assignment of the position of channels in a 1 D 2 D or 3 D display When no map file is loaded channel displays are arranged by FE channel address Electrode map files use a
101. samples prior to threshold crossing not the spike threshold crossing event itself Note Spike events occurring faster than every 1 7 ms will not be completely captured as Spike event data packets do not overlap o 1 kS s Analog 1k and 30 kS s Analog 30k data streams can be selected for the Analog I O Front End The Analog I O data is not filtered This data is saved in the ns2 and ns5 file format respectively o 10kS s data streams can be enabled for the Digital I O Front End Digital Events This data is saved in the nev file format o Stim data contains the voltage waveforms supplied by the Micro Stim Front End during stimulation The Stim data is acquired in continuous 1 7 ms segments for each stimulation pulse from the time when the stimulation pulse starts until the end of the monophasic or biphasic stimulation waveform including the interphase interval A single stimulation pulse may span multiple data segments if long pulsewidths are used and a single data segment may contain multiple stimulation pulses if very high frequency stimulation is used The Timestamp stored for each data segment is associated with the first sample acquired in the data segment The Stim data is saved at 30 kS s 52 samples in the nev file format Note The Hi Res data stream is the only option available for the EMG Front End e File Name The default file name is datafile The file name for the recording can be specified by typing a name
102. sine wave at a selectable current level of 3 nA 10 nA 30 nA or 100 nA The accuracy of the impedance measurement can also be selected The accuracy is a measure of how many trials are averaged to produce each reported value To run the impedance analyzer select the desired current level desired accuracy and click the Start button at the bottom of the Impedance Results panel When the analysis is complete the magnitude of each electrode s impedance measured in kilohms along with the phase angle which indicates the contribution to impedance from resistive versus capacitive effects will be displayed in the Impedance Results panel as shown in Figure 3 18 The test parameters can be altered by clicking Clear in the bottom right corner to reset the application Note Only the 3 nA current level is available for Grapevine Nano and Nano2 Front Ends and only the 10 nA current level is available for the Nano2 HV Nano2 Stim Micro2 HV and Micro2 Stim Front Ends Grapevine User Manual Document Version R0O1550_08 eo PA k4 Impedance Analyzer a LO File Tools Help Electrode Selection Test Parameters Impedance Results D 100 complete Hardware View Flectrode View Pin Frequency Hz Current nA Cycles Magnitude kOhms Phase k P 000 1A101 1000 10 100 103 4 F A 1A102 1000 10 100 10 2 3 EE Current nA 1A103 1000 10 100 103 3 4 7 Por B Nanos 3 nA only 1ALO4 1000 10 100 103 4 nano area 3 LALOS 1000 10 10
103. st recent spikes overlaid in the spike sten Window The Clear Spikes button clears the display Sorting Enables disables spike sorting for the current channel The Clear W Enable Units button clears current unit definitions for the current channel Clear Unis see below Grapevine User Manual Document Version R01550_08 38 ERS E Document Version RO1I550_08 Chapter 3 Trellis Software Suite Configuring Threshold Parameters Trellis supports setting both positive and negative thresholds for each channel Thresholds can be set independently of one another and can be turned on or off at any time Spike Scope provides three ways to set thresholds Note Threshold changes made in Spike Scope affect only the currently displayed channel not the global setting 1 Dragging the red positive or orange negative threshold line to the desired value Note Dragging a threshold line to the top or bottom depending on whether it is a positive or negative threshold will remove the threshold 2 Typing the desired threshold in the hi or lo threshold text fields Figure 3 11 3 Selecting the auto button will set the threshold using the auto threshold algorithm See the Instruments section of this chapter for more details If threshold mirroring is enabled any changes made to a positive or negative threshold via methods 1 and 2 described above will cause equal and opposite changes to be made to the other threshold
104. switches Table 1 4 Grapevine FE reference selection switch states Switch 1 Switch 2 Meaning No reference is connected the reference is floating Down Down Not Recommended as it typically results in increased noise Reference pin R1 is selected as the reference ole electrode Reference pin R2 is ignored Down Reference pin R2 is selected as the reference electrode Reference pin R1 is ignored Reference pins R1 and R2 are tied together and used Up as the reference electrode This can be used with a compatible adapter to create a reference bus Grapevine User Manual Document Version RO1550_08 Eta ERS Digital I O The Grapevine Digital I O enables the Grapevine NIP to interface with LVTTL digital devices The Grapevine Digital I O FE has 20 inputs that can be connected to the digital device outputs Signals recorded by the Digital I O FE are synchronized with data recorded from other Grapevine FEs All digital inputs can be used for triggering various functions of the Trellis software suite The Grapevine Digital I O has 20 outputs that can be used to trigger other devices All grounds in the Grapevine Digital I O are tied together internally minimizing grounding issues The front panel of the Grapevine Digital I O contains markings and labels that identify the Grapevine FE as well as labels indicating the function of each port The Grapevine Digital I O comes with four mounting holes that allow the device to be mounted to a wall
105. t definitions Spike Scope can be launched from the Trellis Applications tab or from within Raster or Spike Grid by double clicking on a channel display The Spike Scope displays 52 samples of 30 kS s data or 1 7 ms starting 15 samples prior to threshold crossing Note Spike events occurring faster than every 1 7 ms will not be completely captured as Spike event data packets do not overlap Spike Scope elec B 1 0 Signal History Display Range Thresholding Q 5 95 s MULJA zt 200 uV We W E o 160 OM tius Shur KI aati 20 uv DKV IZ Figure 3 11 Trellis Applications Spike Scope The Spike Scope contains a variety of controls to customize the view of this application A graphic and description of each control is explained below Current Signal elec A 1 1 4 Specifies the currently displayed channel e When enabled Spike Scope will track Trellis focus Ge the last GE selected channel in Raster or Spike Grid raw Increases or decreases the scroll speed in the spike filtered signal 2 605 Ml display window Display Range e e Increases or decreases the voltage range of both signal display i 200 uw We lV windows Thresholding l Afe aw Specifies the current channel s threshold values see below f lo lt 97 88 gt i Toggles whether the high and low thresholds are mirrored see below Spike Persistence Specifies the number of mo
106. t panel contains markings and labels that identify the Grapevine FE 32 channel single reference microelectrode NS ee Samtec Connector to electrodes Figure 1 8 Front panel of the Grapevine FE Micro shown similar for Surf S and EMG Front End Back Panel Grapevine Micros Surf S and EMG The back panel of the Grapevine FE contains markings and labels relating to the device certification an ESD warning and a label directing the user to read the manual see preface for ESD user instructions A label to identify pin 1 of the Grapevine FE is found on the bottom left corner of the back panel When connecting the Grapevine FE to electrodes or adapter boards it is necessary to ensure that pin 1 on the FE aligns with pin 1 on the electrode connector or adapter board Additionally the back panel contains the switches to select a configuration for the reference pins except for the EMG Front End which has no reference switches Reference selection 1s explained in Table 1 4 ripple i SN R00249 0001 reference AN g A selection Aes switches See Manual TYPE CF i A Gef Pin 1 Samtec Connector indicator to electrodes Figure 1 9 Back panel of the Grapevine FE Micros Surf S and EMG Note The EMG does not have reference selection switches 15 Document Version R01550_08 Chapter 1 Hardware Overview ES Front End Front Panel Grapevine Nano FEs The connectors pin 1 indicator
107. the leftmost circle of the control the one containing a and repeat the steps outlined above Sorting Spike Persistence Sorting Spike Persistence E Enable 50 E Enable 50 Clear Units i Clear Units Clear Spikes Clear Spikes Figure 3 13 Trellis Applications Spike Scope sorting defining a hoop An existing hoop can be modified in two ways it can be moved to a new position or it can be expanded in the positive or negative direction To move the hoop click on the vertical bar the cursor will change to a clenched fist and while holding the mouse button down drag the hoop to the desired location as shown in the left panel of Figure 3 14 To change the hoop height click on an end of the hoop the cursor will change to a horizontal bar and drag the end to the desired position as shown in the right panel of Figure 3 14 SAIT 200 ON M 400uV f T 4000V GD OM Ka 600 uV Figure 3 14 Trellis Applications Spike Scope sorting modifying a hoop Grapevine User Manual Document Version R0O1550_08 40 ERS Hoops can be deleted in three ways To delete an individual hoop drag it to the trash bin at the bottom right of the spike display The trash icon will turn red when the cursor is in the correct position for the hoop to be deleted as shown in the left panel of Figure 3 15 To delete all the hoops for a given unit click on the right most circle in the unit control bar the one containing an X Finally all hoops on
108. tterworth characteristics by default and are applied to the data streams at the processor level Notch filters can be applied to the Hi Res and LFP data streams to remove common noise signals such as 60 Hz AC line noise 50 Hz in the EU and other countries The Notch Filters have 2 order Butterworth characteristics Custom filter settings can be created by contacting Ripple Support Filter Settings NIP 1 FE AcL 15 375 Hz EMG Custom Figure 3 6 Trellis Instruments Grapevine NIP configuration Grapevine User Manual Document Version R01550_08 ES ERS E Document Version RO1I550_08 Chapter 3 Trellis Software Suite Applications The Trellis software suite contains five applications that provide a range of functionality including real time visualization of data streams measurement of electrode impedance and control of electrode stimulation These applications are Raster Spike Grid Spike Scope Impedance Analyzer and Stimulation Controller Each application can be run by double clicking the icon within the Applications tab of the main Trellis program The settings for each application can be configured to restore from the last time the application was loaded by going to Tools gt Options Detailed descriptions of these applications are included in this section es Trellis ex o dm So File Tools Help mm trellis Application Info Ov E Raster E Description Za Ka Provides real tim
109. ually e Speed 1000baseT e Duplex full duplex e MTU Standard 1500 Additional third party firewall programs such as Little Snitch should be disabled Note Users of Mac OSX 10 9 and above should disable App Nap globally This can be done by opening a Terminal from the Applications Utilities directory and entering the command below Be sure to restart the computer after running the command reference e defaults write NSGlobalDomain NSAppSleepDisabled bool YES Linux Network Configuration The exact method to set up the network for the Grapevine NIP varies based on the Linux distribution that is running Below are the settings that must be established through either the appropriate setup tool or command line For Ubuntu 12 04 the Network Connections interface can be opened by entering nm connection editor in a terminal window This will open a graphical interface similar to Figure 2 5 where the network settings can be configured e DHCP Configuration Static or Manual e IP Address 192 168 42 129 e Netmask 255 255 255 0 e Speed 1000baseT e Duplex full duplex Method We e MTU Standard 1500 Addresses Editing Wired Connection name Wired Connect automatically Wired 802 1x Security IPv4 Settings IPv6 Settings Address Netmask Gateway Add 192 168 42 129 255 255 255 0 Delete Search domains Require IPv4 addressing for this connection to complete Routes KR Available to all users Cancel
110. und connection Details of the pinout configuration can be found above in Figure A 5 same configuration as Analog I O input The analog outputs can produce signals within a 5 V range with 16 bit resolution 0 15 mV steps The analog outputs have an impedance of 5 5 KQ and are produced at 10 kS s except for the audio outputs which have an impedance of 1 KQ Contact Ripple if custom analog output configurations are needed See the Instruments gt Analog Outputs section of Chapter 3 for detailed information on analog output routing of continuous data streams Audio Ports There are two stereo audio output ports and one stereo audio input port on the Analog I O FE The audio input port can be used to capture analog data with a 5 V range The audio output ports can be configured to reproduce data captured from other FEs such as spiking activity to be played from speakers Grapevine User Manual Document Version RO1550_08 Zo ERS Discontinued Front Ends Surf D Note The Grapevine Surf D can still be used with all Grapevine Processors and Ripple Software applications The Grapevine Surf D PN ROO263 uses a 36 pin Samtec connector PN SFMC 118 L1 S D mates with Samtec FTS FISH or TFM series connectors to interface with electrode platforms using the same pin configuration as the EMG shown in Figure A 3 Because the Grapevine Surf D has high input impedance it is suitable for connection to all differential surface electrodes Dif
111. ununnnnununnnnunannnnununnnnnnennnnn 72 El ctrode Adapter ROG enia iain daesntenciecte neat 72 Analog I O and Digital I O Breakout ssssusussusunnununnunnununnunnnnunnnnunnununnunnnnunnnnunnnnnnnnnnnnnnnnn nnan 72 Recording Front End Tester EES 73 Stimulation Front End TeSting csssccsssssccseeseeseessessneseesseneessneseeseessessesseseesseeseessneniessnenaesas 73 APPENDIX F TROUBLESHOOTING SHEET 74 APPENDIX G REVISION EISEN 75 Grapevine User Manual Document Version RO1550_08 Di om Se Chapter 1 Hardware Overview How to Use This Manual The chapters and appendices m this manual provide an overview of the Grapevine system hardware technical information on the Grapevine components instructions on the installation and use of the Trellis software suite and additional information on system configuration This document is not intended as a service manual The specifications and diagrams that are provided in this manual are to assist users in understanding the capabilities of the Grapevine system Only authorized Ripple employees and representatives are qualified to repair any component of the system All rights of the warranty for said component are voided if any other party modifies any component without the written consent of Ripple Grapevine Neural Interface System The Grapevine system is comprised of several components that can be used for the acquisition of electrophysiological and neurophysiological data At the heart of t
112. vents out of individual digital output ports The Grapevine Digital I O has 4 SMA output ports and a 25 pin Micro D output port similar to the input side See the Instruments gt Digital Outputs section of Chapter 3 for detailed information on digital output event markers Note Only one Digital I O Front End can be connected to an NIP The Scout Processor has a built in Digital I O and cannot have additional Digital I O Front Ends connected BI Document Version RO1550_08 Chapter 1 Hardware Overview EES Micro D connector to grapevine NIP hole SMA digital input ports 1 bit per port SMA digital output ports 1 bit per port digital i o ripple i Micro D digital inputs 16 bits amp 2 strobes Micro D digital outputs T bits amp 2 robe S yndul jajjesed parallel output Micro RS 232 RIGHT I O port serial port hole Figure 1 11 Complete set of views for the Grapevine Digital I O Front End Grapevine User Manual Document Version R0O1550_08 18 ERS II Document Version RO1550_08 Chapter 1 Hardware Overview Analog I O The Grapevine Analog I O enables the Grapevine NIP to interface with analog devices The Grapevine Analog I O has 28 inputs with a 5 V range that can be connected to the analog output of other devices Signals recorded by the Analog I O FE are synchronized with data recorded from other Grapevine FEs The Grapevine Analog I O also has 28 outputs that ena
113. with a 500 Hz cutoff The ADC of the EMG FE has 24 bit resolution with 0 022 uV bit and an input range of 150 mV Each channel of the EMG is sampled at 2 0 kS s and has an input impedance of 1 0 GQ in parallel with 20 pF capacitance with input referred noise of less than 1 0 uV RMS Note that G indicates the ground sockets and N C indicates that the socket is not used The 2 kS s Hi Res data stream is currently the only option available for the EMG Front End APES PEEP PE SS ee EIS Figure A 3 Pinout configuration of the Grapevine EMG Grapevine User Manual Document Version RO1550_08 ES ERS Ey Document Version RO1550_08 Appendix A Technical Information Digital UO PN RO0S591 Size 105 mm x 60 mm x 14 mm LxWxD Weight 100g Digital Inputs The Grapevine Digital I O PN R00591 is capable of recording up to 20 digital inputs On the left side of the Grapevine Digital I O are 4 SMA connectors and a single 25 pin Micro D connector for parallel digital input use Each SMA connector can receive a single channel of digital input whereas the Micro D connector can receive 16 bits of digital input The remaining pins provide ground connections and Strobe synchronization Details of the pinout configuration can be found below in Figure A 4 Figure A 4 Pinout configuration of the Grapevine Digital I O Micro D connector Note SA and SB stand for Strobe A and Strobe B respectively By default the 16 bit parallel port st

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