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Software Operation Manual

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1. Instrument Menu The karg shows he erone menu and describes the options Varian LCMS The coriigured instrument NOTE Entar intonation in instrument Parameters e Configuration sereen Conliguraion Adi new mosu Remove Module Names Remove any associations between Modul Names tnd Modde Adresses This alls youto connect a Modo win a dilereni Motie Name ar trat address The next Ime e associated Moda connects at tha adores you w be prompted 10 ssec e conect name fr Made o the existing instrument Instrument 4 Faults View aut in a madue tin Instrument Use the update ey to crack tor new Tauts witout apeng ne log box rem iat in Setup Ethernet Communication Setup communicaton between the 450 60 HAST GC or ter Ethene modules and System Canal Setup COMM Ports Set up communication between System Contol and modes communicating wih e Worksaton tough fe Serial ports on e PC The test ime you sar System Cort the lr Convmuncaton Coniguaion Ward stars utomatcal Windows Menu en ae E pono Show Module Windows Display he contigured mode windows in is ample he maces we he 21210 Pumps he ProStar 430 AuloSameler and the Quad leonize Module Windows Create icons of indvidual module windows at tne Bonom ehe sereen Show Automation Windows View the open automaton screens The posable ae SempleL s Roca and SequenceLs leonize Automation Windows Create so ion ot a
2. EREEREER H pere samena ie Kos EE lL les a E The Caan abie has sling ei nta me Ret temal Standard S Compound D Quanta lon Quan nj Calevatens integran Wentteaton and Referens Specrum Ret peci Cick Select Data Flle 1 open a daa ti Son Add Insert Delete F Down or Restore compounds Other functions are Buld Compound List inport Compound List Export Compound Ust ar Pin Belore processing baet tles review the compound table o check Doubles a celi to open he Compound Tab view In tis varas Ret 7318 was selected Compound Attributes The folowing shows the Compound Attributes lah view fr the retention tine 7613 was doute cictad The Civorrarogram and Specbum Displays are on op aoa ihe boom part haa fas Ad raton suo aa Compound Name and AS Number during ie pea ation process Edit Name CAS Number Retention Time Eat the Compound Name and CAS tts to describe your compound pet te chromatogram pay t update he Renten Timo The spectrum of he sacle scan 12 dupayed I becomes the new eteronc soectum You Select when you ext he tab disc or cick Next o Previous to move a a Seren er Identity Internal Standards By defaut conpounds are idend as Anales enya teal Standards by sec he neal Standard opten barion in e Compound Type secon ick Ner or Previous a check me Compound ID es mor man one tema standard e r
3. Select Activate Method tom the Sample Type cal lk AutoLnk in 1e row at you are working Enter tne name of he Method or cick Browse 10 select the Method om a lst Spec any number of Methods in the Sample Create or Edit a Sequence Tous odia eqns do o ttg ume Reems sout es Riana lt Ope an hen Sequence vom the Fe monu ew Aa to add ines o me Sequences Enter the required number o Sequence tnos Select tme Actlon for he selected step ick Browse to select te Method and Sample istes Compound Table Use Compound Table tor automatic quantitation wi extemal or internal standards The Compound Table Is pat ol ne data handang method In ie Memod Bulder vee cick Compound Table unde MS Data Harding Clck Show Hide Director to splay he method tul screen rrr Sizge SSR ae EEE Creating a Compound Table 7 Cick Compound Tabie 2 Cick Select Data File cick the data tle of your choice and cick Open File This data Te becomes te iteence Dala Fle 3 ick Build Compound List 1o open e data tle In MS Data Review 4 Clekihe apex othe desired peak in he chromatogram to generate a Specia and pace niarmate w he Speci Lit 5 Close MS Data Review E Glekimport Compound List and elek te paca trom the table and then Sk Select The compound is added to the Compound Lst NOTE pape lk aceto spy more enian WC eet ver Ne URN
4. NOTICE Varan e was ace by Ait r n Mey 2010 This domes pride corte buts no ger kapt arent and dus wl cont Nora ret akr goto wor Walnut Creek CA 94598 16751USA 300 310 320 MS LC MS and GC MS Quadrupole Mass Spectrometer MS Workstation Version 6 Software Operation Manual Trademark Acknowledgments aap and Windows are registered trademarks o Microso Corporation DemoShield and isallek are registered vademarks of stalSeid Corporation Acrobat Reader Copyright 1984 2010 Adobe Systems Incorporated A ius Teserved Adobe and Acrobat are trademarks ct Adobe Systeme Weorportd and may bo registered in certain rn ner brand and produc names re ademas or registered vademara of their Tespaciue owners Copyright Vara Ine 2008 2010 AL rights reserved Contents Introduction Gere Workstation Toolbar ESS Workstation Tops Opens System Control renee aeae Quad Status and Conil Window a AutoTune Adtoune Onions E Tune to Target Ratos 3 Siandard Compound Eda E ao 45060 Med 431 66 Methods si equation Metod s Seting up MS and MSS Acuna r4 Scheduling Tinea MRM Transtiona 72 Injecting Samples ee S Shuidown or LERS m S20 LCS Syringe Pump and Vale m LEMS Manus injectors m GENS or LEMS inecing Single Spies r1 iasan rj CMS or GENS niacin Malis arez ss Montor Aun tau s Automated MS Rap neraet r4 Stopping an Acauiton F1 Seta Colect Delay
5. Quit Macros Stap any MS incon in progress ser Ph Erter up N 10 PMs macros witen in Paw Macro Language aed feat aed e Pun a HN aog he conesponing smiey tace in the toolbar CSS ETE Piot te readbacks Pio a graph ot me alamna he TIC the area o 2 masses reads ora Lo menlor umen stalus aic he In use chs bo ine checkbox hito he deed parame and en cick ABB go pem Tires RT Analog output For service use on MSMS Breakdown Optimize the colision energy fr any given parent mass Used pany tor LOS Bekeout System GCMS Heat the source to 200 C and ne mantold at 0 for 3 hours Man coola he system to ia conditons Ao pol a graph or ne source and and tenperatues LCMS Heat he API manftd o 65 and he MS manifold to 52 or Shouts and than coo ve spem to intial conditions Ao piots a rach of he source and manold temperatures Overnight Standby Turn o al gases and sts the MS to a ow scan mass Troubleshooting Run various arpes procedues See the hardware Inst setupiothers Perorm inti Instrument setup operations Coarse tune Sekce use oniy Calbrae pressures Calbrates he MS EFCIEPC pressures Evacuate caigas bulb Flush caigas ini th sore tor several minutes Do his racer th clos bub was removed and essa Cool down and vent Cool te source to 100 C hen turn ott the tore ampia and achats a solenoid owing ar la ear ne sure Reports Select a pent vi
6. TOTE The Raised hea vo Sawaya HU ard cael be EU A the Total te cannot be edited a ma EHE qus Injector Oven Tu peer oven ono set e lector temperatu sl stae adsit Column Oven Stabilization Time The test ow of the spreadsheet contains ne intl termperature and hold ime The invumert equlibrates 1o ths seg wren a methods scale and i Tesiored 1a 1a seting aer sach cvomslograg un e comglted The folowing rs contain me programmes stings tor each ramo segment Each segment ramps the specie emperatu ate spected ao fasauming We rale s achevatl and hen ade ne temperature forthe Speed te TOTE The Rams Tei ere WaT Sawaya Bah ard Sal be REG AO The Total tet canat be ated pest fet ts en eL ce al GC Autosampler The GC autosampler window has tour sections which are similar tor tne 6400 ana ne d 10 AuloSamplers The parameters rede re samping yige sze Paean deoin tor Defaut clean steps are used i automation is stopped or a serious fault is detects Select Pre nj and Postini irecton singe clean modes e EA ceu Se E EC eee E E EC s ee Injection Mode There are seven recon modes Standard On Column Standard Splits Neat Viscous and Vala See online Hol tor a detallad description ofa Injecton Mode parameters eios Hoe TTS Editing 431 GC Methods Cek ne Method buton in te 431 GC Status and Contrai Window te open tne anive Merod and
7. completed The folowing rows contain me ime programmed stings orth 1177 Spt alo Me Spit Valves swizhod o be rato 1n each row at a indicted be The flowing is a typical program for a spes injection A low Spit Ratio conserves caer gas wie keeping contaminants Tom baling up in ie eco The injecter s eot spiese or tout 1 minute so ar a anaes fected can locus on the oium A Split Ratio of 10 afer inject shes Tesal sovent rom ne ijecior H c ow Ts one Flow Pressure Window The Flow Pressure window can have up to vee Electronic ow Cantal EFC Programs Front ae an Rea To set up an EFC do the talowing 1 Use the abe to select ne tront middle or rear EFC Program 1o edt gt Select ne type of EFC at each poston or none ina EFC is installed at that postion ps Type 21 EFC for 1079 1177 Injectors The Fw Pressure window or a Type 21 EFC has a pressure ramp The test ow ot the spreadsheet contains ne Inl pressure and hod trme Aer he method e Te EFC equates toe seting and ater each chromatograph runi compito s restored 1o ths set The lolowog rows contain me programmed stings tor each ramo segment Each segment rampa 1o he speed pressure athe spctig tate assuming The rate achevate and ten old tha pressure forthe spected ime TOTE Tre Rams Tei T RW avons aways BK ard cara GU AG The Total teid canat be died na we Enabling Consta
8. ample Close he Workitaton Toobar System Control Overview Thi seton presenta an overview of System Conta unions Other ooer are eslanad n detal in atr sections Doe ck anaes on the Contguson sereen to view the Instrument tats and Control window i Instrument Window Use he instrument window to mony he stats of modules assigned to he estumont dta acquitn inject ons or more samples and do balen Pecskuat ns Slang athe Ip of he window haze fcaures are System Control Menu System Conti Toolbar Quad Status window and various deni aig maestus e Bir Activate Method Select an existing method This sets the conditons for he connected mogles suc as LC GO MS auasampir and data handing Upload Active Method trom Modules Save ornare fom te existing miduee ia new oroxstng mood Ths meld becomes a New SampleList Creato a Sample Open SampleList Open an exsing SonpleLs New RecalcList Creste a recalculation lst Open RecaleList Select an eetng recalculation lat TOTE Process He System Comal ar m HS Data Review S Data Review rom the Quanltaton mers select Process Review Recalls ough Processing Resales akes longer om System Cant you can use Autolak incon nung automated reporing wah temper such as EnvioPro ToxhraPus and Mutcompound Repons New SequenceList Creat a sequence a Open SequenceLit Select an raina sequence ist nen fora H Prin
9. generated I selected in Repor Method section When ou disable Siomated printing automaton continues but no reporte ar ted ue EET Stopping an Acquisition During automation and whi the LCMS or the GCS system is running you can suspend ar stap data aequsiton hom th Auiamation menu in System Canmol Suspend Automation Suspend automation after me curet aun s completa Stop Automation Stap the curent nun reset ne modes and suspends Resume Automation Resume automation atter suspension Reset Modules Stop he curen run and reset ali modos Automaton Proceed lo e next injection aner ali ne modides go to the Ready siate ne Aquin was started tom Start cotecin data IB me vae Toolbar ciek St p MS t en dra acqussion or In the Automation Menu bar ick Stop Automation maj pmo o m Set a Collect Delay Use cole delay to ta data acquisitan ater the sample is injected A Separation can o set up a remove bute om me sample The savent ar butler Ean be diverted i waste and data colecton oan b stared aner he vale nes to ring tre Tow rto the MS i The irst 30 seconds of a separaton ta colton can be turned on wale un lined compounds Paare natal tees re wasned of he cum Data colection can alari when the moble hase congestion changes andthe sample beg la e Lems Divert he LC efuert away trom the API Source using he 320M 6 port valve Frequent he soven ront contain u etaned non volanie sate tam e Sam
10. peta wenn net ns P te Sat at sare en gt ne We ene tut dump estere merm eim pod RER aasre PEE arben E eee E rx NOTE The sqreataheat mus ave he CRS Nune Compound Name and isi Mese courne You do net need To errer values Using tMRM with a New Method To create IPM vansitons in a bank trate complete the falling wo pat E Create and compet anew spreadsheet template using the C Spreadsheet erate area 2 Import the spreadsheet into a new method using the Import RM to Sean M ca area n Creating a IMAM spreadsheet template To define MAM transtions in a new method do he allowing Olek Make template in the Create a MRM spreadsheet template area 2 Erter a tie rame and cick Save A tab lite tenpiate fle is saved inthe Varr merca or 3 Open Excel and thon open the te Solo Text Fles in he Fits af type feld in the ower parto he Open Peers See tn harlot gt The spreadsheet nas ne folowing tlds The CAS Number Compound Nam ard he Last Mass columns ar required You do nol have 1a put data in nese te elds e cer eis must be compte CAS Number A sig containing the Chemical Abstract Services umber ore compound Compound Name A ating descbing ha compound Polarity he poat tor acquisition method c Use or or Use the number o tor postive and 1 tor negative QU Frat Mass The masa tor Ot mz Q Last Masa Use is orly if a mase range isto be tered in O1 m
11. 08 RENE Inte al ston Volume nd etos Usa ats eter e appropriate The teld beginning wth Ant St is oniy tor data tles acquired wih standard GC Vecie smale urate ated Ercan el Select he Method tor the run ick Browse 10 select a ie Other Fields Add Aide a newe Ine to the endo hel Insert iners a new ine betore te curent active Ine Delete Deletes te curent active Ine Fl Down When sections ot columns or entre ines are highighied the formation n e ap pied row repeated one lowes sci rouw Add Lines Ad several similar Ines 1o the Samples the common fomaton a he dag pox Felden Add Lines may vary rom one auiosamgier orancmer Ony te Teide associated wth Ine selected autosampler are splayed For sequently numbered Samgie names check the box and erter the sang urbe ane number ot entras a aad 10 me Samples Ine Sampie Names M have ese numbers appended to them Defaults Change the defaut entries in the table such as irjecton Volume injectors Used ete Aer dering new aetas ick Save to atval he new ors Dats Files Change he eger and name o me Data ie Recolckit Cick and generate automaticaly a Recaletist associated wih singe sample acsuetore Specifying the Data File Name and Path Data Fle names can be up o 256 characters ong Sample ID injection date due nane and nection number can be embeded m ha e name making the Data Pus hame correlate wieso sample eco Click Data File o open the Data Fli
12. Creating a report doe not change a tue Ne Tune to Target Ratios Mody existing tune fles See Tune o Target Ratios on page 36 Calibrate Detector EDA Optimize and callate the detector voltages over the tre dynam range Run e routine perodcasy and aiet replacing the eec The deaciorealraton rus has wo parts The tyst determines he nna detector at tor ne maximum gan EDR Macon Te second osibrates tect gun as a ncn lage tor a Sitom detector susteni mode o Extended Oynani Range EDR EDR aubomatealy apata ha detectar votage t accommodate ner smal ar large on gras over he entre ni range ol e mass spectomete The folowing gure shows detector gain a tncton d detector vottage Stop Tuning Abort the Turing proceduras and restare Te mast recent Tune Ne Analyzer Tune each quad indvidual or tune botn Use te dtl Both Seting fv iple quadrupole instrumente Y using a vile quadrupoles a single quadrupole seeti he quaduple ise Polarity Set AutoTune polary mode cus Ci AutoTune either postive or negate EL AutoTune postive on toms ESI AutoTune ether postive or negative APCI uses ES tune e lonis Select ener a spec ian cr i deed lans in the Compound Is it choosing Single tam the et elec an an om a latot ons nthe Standard Sompura Using a singe o sd a new data point ta he loaded tna fle and Soet nar change casting ponis i he same mass la present tuned and hanged according A raport far e single mas
13. Dag a 1079 chr ovar coolant is rad in te arive Diy he 1079 meer oren programmed temperature seting in Dogs ho actual tmperatre at tne 1079 jc oven at te Ser orror Dogs te curent satt siste otite 1079 iere moe Ems re 1000 e O Boum e anen ni T soit rto is stne aste ae biete 7 Bana ta tempere at wich the costis era Rem Beserption Dip te programmed ire t vue inte see met ei Ye e cort me ct perd mea 1041 1061 Injector Status em Description SPT Injector Status em Description Taenia aaae has lad tor he oven ipe 21 and 25 Injector EFC Flow Pressure Status nem Descrpton easy Yes stre sepas are roche and sisie Essent eng ard mera ara Sate nro ns S curent spit raio of te 1079177 ejt method The pte cra ears riw Re Powe Type 23 Injector EFC FlowiProssure Status tem Description car gs coun nigh ard eral dant Type 24 Injector EFC Flow Prossure Status tem Description E agis cem hend prese str inte mot um Tye actuat con had pressure at he 460 6 our Faw Baray CE me cnm tow rate euaid fom fe aR nores coun legi na net veen stn ne 0 Se spa programmed a ow ate he sve mats Tie Vey neta mers fe ew ey uae Tar TE edes rir gu colo t
14. Module Control Parameter By changing the module address i he parameter window a memod developed of an eument using one module address can ba easy aate Iran inerent module adress The 450 00 Method section has eight windows anns Sample Detery n FowPressue Column Oven Detector Mr Data Acquisiton Each wdow has parameters tor aiaa he GC during a chromatographie fun ithe 300 Seres MS tho aniy detectar ana o samples are jected by Syringe ine Sample Desery Detector Output and Data Aequalion sections do at apply ForieGrmalon on those seckon se e 0 GC User Manual Checking Method End Times Eating windows except tot me Column Oven window and Detector window dcl te current and Ime of tne column oven programa the boom you enter program end mes tor parameters ther an he column oven tnat ened tha colum oven ond ima he losing waring i displayed When he Method i donioaded to the GC component program end ime ceeding me column aven end ime are Wuneaea Injector Window The neoa window contains up to ee Hea Programs Front Middle and Rear The sobermal 177 and temperate programmable 1079 ects can be used wih the 300 Seres MS Use ico Programs 1o spec the Temperature se pot and apt rato othe sothermal 1177 sect and to Spey the temperature rame and sit ao of mo programmatie 1079 ecto 1079 Injector To contol he Oven Power and the Injector Coolant trom the Injector
15. a does not contain Appian or Guik k butone Sd dag the toa to me prefered edge ot the sereen The next ime you open Wonton e tober wil be atat spot Workstation Toolbar Menu Put me cursor over an area ot he Workstation Toolbar without an application or Quirk burton and ro cick display he men Move to Windows Taskbar Display the Workstation Toolbar as a Windows Taskbar icon Clck Move to Windows Taskbar rom he Workstation Toolbar onan menu a e Taskbar icona apea ine over ight ofthe Windows Taskbar Cro ihe Workstation Toolbar con to display he optont menu Show Hide Applications on Toolbar Select the appicatons fr the Warkstatan Toobar Thea sets he separa shown on te tba ard 1e rv ct ha To remove an icon or ne toolbar select fom he op lst and clk Remove Toss an leon to me toolbar select om the atom lat and ciek Ada EnabloiDisable Instrument Modules Enable nstument modes When an Insvument module s valable appears in System Coriro rhe conesponding nstumen connected and une on You can create a Method secta EE hows a instrument modules Pat are carey instatag and enabled Th lt on eight shows al inatunent modos iat Insta bu no enabled Todasbie an instrument mado select trom h st on the teft and ok Disable To erable an instrument modu select t trom the lst on the ight and ek Enable Application Descriptions pup B massi nee art When checked oaen ct an applet
16. device suchas a LIMS system Use the User input Traces to aeque data tom another detector such as an Evaporative Lo Scaterng Detector Res eee em rum e peu d Emm i so Ese PMLs Paw Macro Language MS Workstation provides PML macros you can use betore or ater a tme Segment Madly and creare PUL rough Tois ine Quad Module Vew pem ma T l Cl Gas Type Select the Gas Type You can also spec another gas that is configured wth your instrament remm ESI and APCI Valve and Syringe Pump For the S24 Use hes prt valve or the syringe pump or both The set valve can be used in Manual Diverter or Injector mode Set me Now ate for fe Synge pump iecit tere inn C eee L u rena eran mena TE ce L ESI and APCI Drying Gas Use he SelacTemo testure to ensure me optimum drying gae temperature for he matsie phase composition of ESI or APC uns mare Tse ratio wi sree E ramp SW OTR and here i grant weh a temperate override he temperature qoos loo Setpoint for hat segment The ramp continues aner to segment s completed APCI Vaporizer Gas Temperature Ramp Enter a vaporizer ga temperature program o ensure the opbmum vaporizer Temperature tor APC nar umane Tape pem me gae DIP DEP Optional GC MS lon Source Enter parameters or ier deact insertion probe othe rect exposure probe See te DIPDEP manual for more norman Schedule MRM Use timed MRM vansitone MRR va
17. edt ead sectors Ater eng he Method and coin e Matos Bude inde you are promoted react me Method Reachvang te Method dounicade he langes to ha Med se Acquisition Method Click Acquisition Method nthe Method Builder ree at aa s 7 You can set scan eare and define MS experiments The Mass Lat has a cleri makes completing e able he using a spreac Si Seat a el mn Method Specifications The folowing describe te Method Spectestions inthe Acqustion Method Mode Select your instrument Modal Ionization mea the lonzaton mode Elan lonzaton El Chemical lonzaton CI Kerr langaton ES or Amosphete Pressure Chemical lozation n Method Run Time There are two selectors Select Une run time to use the run time in fe metto a Type in the minutes to use a time other tat in te method Ifyou do rot ror a run ime tb data acquired unl the end teme e rea hed in al ras rune Data Typo Set me scan ye Cento or Pro NOTE aa cen he done T or Cen ue Collect Delay Thi teature is uso tor applcatons mat have an inti clean up sap To delay data colection afer tne run starts do tne folowing 5 Check Use delay 2 Enter a teme In minutes The delay tne begins when the run starts pus Display File in Chro Enable to display data is in he Cro view Y Detector Use EDR Extended drame range he detector automaticaly adj
18. ja ina aquired over e mass ranges scanned Chromatogram Real tme or post acquisition deplay af chromatograms Picture Display program tor graphing and araning using Paw macro commands Instrument Status Graphic ot he analyzer wh temperatures and presses Readbacks Rea me tex depisy at ether anslog or dighal parameters Feces vara or a dapi and Peude ges Temperatures pressures and analog and dig seal values Moner fiestas e ted ne Piet view as a lnci n fme oiner variables You Can mude Redback a ace in a dala e Reaciacks ars updated aou anes very second Caution Rescbask values are updated once every second whereas the RF and Some D voltages vary sigritrartiy taster dung scan ot dug posne Syachino A a rest he oly meaning teadba values ol hose fast veving rameters are cbtanad wren he scan is zoomed n on one mass and when fere i no poat switching Probe Real tme text and plot splay f elter the DIP or DEP probo which is n option fo ether 3204S or 00 systeme AutoTune TOTES The Fare nar Ra a deed r C Ru Tune AutoTune opimzes the sensi resolon and mass calralon othe For LOMS a reference compound such as polypropylene geal PPG is used asa uning soluan A custom tuning soluson can also bo used For GCIMS FC 43 is the tuning saluton A supply bul Is inside pe tront panel Each quadrupole can be tuned in both polarities A repon wth a display ot pea Shapes and imensites and a text report win unig detalls are depl
19. maha int more segments gt Enter the Maximum number ot segments This tha maximum number of Segmente MRM uses to ope the menod The maximum value ie 60 Creating tMRM transitions from an Existing Method you nave an xsi method and want to make MRM barne complete he tolowing par procedure 1 To make a spreadsheet rom an existing matnad and eter data to complete use me Exporing Scan Method te he MANM spreadsheet area 2 Inport the spreadsheet into a new mathe using e rara MANS o Scan Method area Exporting a Scan Method to a IMRM spreadsheet From the Schedule MRM window clek Browse in Export a Scan Methodi A MM spreacshet area 2 Enter the tle name af the existing method wih the MEM vanitns and han ciek Oper 3 pa Expor to save me MANS as a tb dlisted et tle in the Ya draco Open Exc and men open the tie gt Select Text Fles in the Fles at type feld in the lower part of he Open Se coe cena e E gt Tre spreadsheet has te folowing heids The CAS Number Compound Nan and he Last Masa columns ae required You do rol have oput data rede re elds e cer eis must be comglted CAS Number sting containing e Chemical Abstract Servis number ore compound Compound Nama A sting deserting he compound name Paty The polarty tor acauton method a rara Use the number or postive and 1 tor negative O8 Prst Mass The mass tor O1 m2 O1 Last Mass Use tha only Ifa masa range isto be fiter
20. om the exi viai positon Advance You can wash me syringe in between njecions Clean amp Dup or Clean amp Ab Inthe folowing exemple 1 0 u of Sangle 1 fram val 5 is cid by irjector here It 20 seconds delay and 20 pE of Sample 2 tom visited by acr 2 A separate data fie i created or ach sample Combi PAL SampleList baen to Generic Samples features tne Combi PAL tor GEMS and HTS PAL Tor CMS have setings tor Tray selection Vl postion and injection Volume ihe Com PAL s conquer SPME samping hene nectar Volume llis dsatled and me ber Entraction Time set ie Combi PAL onl secton ot tre Mod is used Tor same exractan A oiher aog parameters are spectedin the Combi PAL section ofthe Mead Default SampleList Entries When you add a new row to a Samples dela values are entered To change them dick Default and ne Set ProStar 420 Sample st Detauits alg bor pens Enter he detrod dof values and click Save Edel Note that volume rack and vial number e not dpi even fan AuoSamper s presen Saving SampleLists for Later Use When you make changes to the open SampleLit me changes are automatically Saved one Sample and are used or me automated runs progress H You want 1o ea a SampieL ist uter nan me open SampeLi vae e fne itomaton Fie Edior appicatondesetbed ine pax section ol marca Using More Than One Method for Injections You can do automated injections using more h
21. one method tor your rector SampleLists in System Control To infact multiple samples tom System Cono use a Sampielist tom ma Ple menu or om System Conto Tocreate a new SampleList On the Fie menu cick New SampleList or ck Open SampleL iat 1o select an eriting oro For more information atout to neida so the Sampleist and cael Fais secon nthe MS Data Haning User Guide part umber 954038 From the System Control toolbar create a new SampleLit ar apen an existing sj Boan B IL Generic SampleList amrer deve s contgured a genere Samplelist opens This Simp des ot have feld 10r i pasos camping options or hardware ontguraon opto The arpa heids are Ike spreadsnootcolumas and can be sized by going se borse Roh ctck a column header to display formating options The Sample Name feld remains fed as you oro to the gh to help you enter Piormatin abo your campis You can enter data handtag parameters such as ihe amount lor slog internat andar cautions Wo underiied peak acit a muliplr an a duoc your requirements are more complex such as multiple ternal standards or mitipie deteciora wich requ aient ntes use e MaChanner Mtns rad Each autosampier has a unique Samples A SampleLit can have more than one autosampler secon so can be used wih dierent autosampiers LC MS ProStar 410 SampleList In addtion tthe Generic Samples teatures te ProStar 410 supports oiher feature such as dierent machen Modes V
22. parameters for ober teatures These vary by ionization mode surat and ine optonal ion sources APCI and POE robe Use overrides when doing MS MS breakdown TE povdes a Sep tar he system o check The advanced options avaliable depend on th ionization mode EI El Ovenides Probe User Analogs vOut Pha CI CI Ovenides Probe CI Gas Type User Analogs O PML Probe ESI ESI Overrides Valve and Syringe Pump Drying Gas User Analoge Ga Pus Me APCI Overses Valve and Singe Pump Drying Gas Vaporizer Gas User Analogs Out PMLE El Overrides Enter values o cuero hose sped in he method tor spect tme segments Inthe botom secin select he unte forme pressure displayed EH ap Cl Overrides Enter values o overde hse specited in he method for speci tme segments veen naal d ras 7 e DRY n SE Sc Bee ESI Overrides Enter values o cuero hose specited in he method tor spc tme segments EISE Parra amas APCI Overrides Enter values bareas hose sped in he method tor spc te segments EL rel d penas T we T mea T eoe T A mes T eene 7 a T a T tpe T mees 5 cma L SS L S e e e gro T Gee 75 ate tad T ee ET ot ined e n 2 oe perla NOTE Vamutrssgnen med wa rape ramo 8 OEE and here i a segrmant wa a temperature ovem he temperare qoos too spon tor tat segment The ramp conteues ate he segment amp completed User Analog In Outs Use he Analog outputs to send te TIC soa 1o another
23. spayed when e cursor Small Toolbar Buttons When Small Toolbar Buon i enable a smaller version of the Toolbar apne Nate athe graphics fr some ears are slg deren IRIN A en Run Application Lt he apoeaton inthe Workstation Toolbar Click to open an appt Help on Select an item trom thi tat to splay Ine online nlp Pick Data File for QuickLink stat he eigh most recen used Data Fies in arder af use When you select a data tie is splayed in e QuekLi buton Pick Method for QuickLink Lat ot me eigh most recenti used Methods In order at use Select one to display tin he Quicklink buton Help on Workstation Toolbar Displays heip tor the apen pata Product Support Web Site I you have tent access and a webbrowser stated on your computer elek 10 Open the MS Workstation Product Support Web Site It has he latest sotiare Sod documeniaton updates or Ihe MS Horstaton sute of products ard netes ips and anoersto equaled questore Vst hate paar About Workstation About has formation abou he MS Workstaton sofware version isalaton alo andato e Instrument modes stalled Tha folowrq an nami he sotware laze you have may be diferent ESI Validate Installed Files Use checksums to test and document errors Printa teport documenting e fe sysem testad he results he date proved and etted and he o me persan logged v loin IDS are wed You can include signatur ine For mor detala reler to onire heip ne folowing is an
24. window do v i Ater turning the coolant and oven on select tne desired temperature m the Enable Coolnt a C drop down Is and ole me desired Coole Timeout mel 2 Type in a temperature ramp program to heat andor coo e 1079 injector 30 The frst yow contains the iti temperature and hold me for he 1079 injector The 1079 equates tah sating when the metnod acted and s restored ots seing ater each cromatoraphe rn completed The folowing rows are foreach ramo segment Each segment ramos to the specled temperare ath spected rae assuma The ae a achat nd hen haida e toporaure respected Ime TOTE The Raised s TON Sawaya HEU ard Caml be ERU AG The Total tei cannot be edad eame as ort then S epe Op CR p mE el ARACET T T an mop i d LZ i mare Ine 1079 Injector is connected to a Type 21 EFC cick Spilt Ratio 1o open the Spit Aano Time Program rei Sn TET e Cmm ER c T NOTE The Typs Zi EFC ral be configured he aa pasion nie Fian Pressure Sedan petore ho Spit Hato can be programmed Specitealy o Duid a 1079 Spi Pato Program a Front 1073 must have a corresponding Fiort Type 21 EFG a Made 1078 must have a corespenang Mate Type 21 EFC Md a Rear 1079 must have a conesponding Rear Type 21 EFC Use he Spit Rao spreadsheet o bui a Sl Ratio Tine Program to control he 107a mec Type 21 EFC comeinaton The test ow of he spreadsheet
25. E MSMS Breakdown 3 Analog Channels E Automation File Editor vere Create Rara Hes Create or Eat a SampleLs Creste or Eat a Sequence Compound Tale Security Administration Gel Passwords Aeplea Lacing Fie Revlon Sening Configuring HPLC Modules Gle Configuring GC Modules Oven 450 GC System Control Command Reference Stats and Gntel Window RRC Window inion 431 GC System Control Command Reference Sits and Cont Window SRB Widow Kerez PML i Ovenden ot PML P1 Simo Commands E Pu Sas ES System Paw Macs fst Help Fes fr Pa E Sore PML Procedures E PML Eto 187 Introduction Overview MS Workstation Version 6 software conr he 00 310320 MS Quadrupole Pstumaris assocate Varan chromatography modules and several modas om che vendors Intonation in a manca a alo on ne Help men Additional Manuals 300 310 320 MS LC MS and GC MS Hardware Manual Th manual part number 9954917000 provides information for using mainiairing and repairng your 300 Series System Tha intormation amp also alia Hep MS Workstation Software Reference Manual This Manual par number 391496900 explains te operation of ha MS Workstatan Sofware These operatons cle deplapng and reviewa hromatographc and mass specal dara tar searching and quanttalue rays Alnough ese orale use Salun 2000 on a Hes hey state e principles of MS Wottaton sotware MS Data Handling Users Guide This Manual par n
26. Gas lakes 5 af ina taskbar opens and doses te vent valve and dents CI gas of in esas area of the ask agen cose Ci vale E he LEAK pmi assis r rom a compound or ms Set te parameters are rens are pated w LEAK pa tr ng vacuum system leaks I sas a series of masses ure used or leak detection First he Pict vew window Pc window i et centoid modes set mass range optimized preveri saturaton and a senes ol ass Me ma user sprays a gas at posse ek locas pet dear T aaar ie pawelgaph widow oper re mee WERT sos ot nae TE ard pel pO pe vmm NT essi mess 25 aap porn pe vaw poston etra trae aa pel porn pe van poston T etes iras 5 aa pot porn pel van postion t lancet ie an goi pe sed pm gets one scan In cento data and one scan of prote data MIXED pmi When Prot and Cert vewa re selected in ha window boh rea me cerra and Profle ata cose Repeat repeat tema below L3 measure sean rium Fat C PML Editor The PML Editor is used tor creating and ding H lt tor the 300 Series oftware The PHC Eo stows acts to me aon PML Nes however a factory PML ie meet wl en be saved as a user PNE the User PML reci Accessing the PML Editor Clck Tools in te Quad mode window to display he PML etor Sepe rome Taie Rl E The PML edtor window
27. Save choose the uber ot produci to save 5 oe Browse to select be method in which to save he produet ions wih the ghost menetes gt Select Apply and hen Start Ema Fed I annie Fhe enn E The breakdown cuves are displayed To ad addtional iane o he metho repeat the procese far te nent precursor ion For example pe SW 780 inh Cine or SW and whatever precursor Ion you chose Forth additonal precursor ion select Auto ind product masses or specity Te product masses To d ins ard colision votages toan exstng method select Append and he appropriate eod One opti ls to create a breakdown cuve by seaming he AP capillary To create a breakdown curve by scanning he API capi do te tolg 7 t Create a breakdown cure by scanning the API capillary gt Enter a range to auto fed product masses or enter up to fve masses 3 Select elhar Normal 05 V stege or Fast 2 step Collecton speed hor S adequate or mast conditore 4 To save the product lons to a method select Enable select Save choose he numberof poduceo save 5 oet Browse to select he method in which o save he product ore wih the ghost innatis gt Select Apply and eo Start 12 a inire ed EIE D A 9 RE erb 9 e oaea arro mj em ome to el The akon cures are displayed To ad aaditanal iane o he matos repeat the procese or ho not precursor Ton For example ype SW 780 in he Ci ne or SW ana water precursor an You chos
28. a Sere AutoSampler Status B sate reat Coro t neoe Tris option ls orly shawn on 431 00 that have an 8400 AuoSamplr or 410 Aitbiector conecta o em m Description Erari var etra 90 or aan ciue Fred up a own Tr cabe ad hep maa age monete ale TT oe E Shove te 8400 Dual Mode Setup Sees ee urs cent The 8400 AutSampler can ct exo wo contiguous injectare nthe same hvomatogrphe un The 00 Dual Mose Setup log lows you t dein the onion 1o be used wen mat capa i voked The B400 can jet ont and me ar mice ana rear injectors and each ot fe injectors can be connected tough an salia column t ary te lated detec When he 8400 AvtoSampler or 8410 Autonjectri usd n Dual Mode use hs log box to aselgn he detector channels 10 ne dea fe lor the corresponding Sample 1n Dua Modo operation we daa Hee are created one or na ta He injected the oer othe second sample nected The sample hat are jeced are dented by hor 61006410 Inecton Postion as eir Poston 1 or Piston 2 The use spactes m he tanie which detector riomalon 1o associate mih cach 8400810 ineo Poston 8400 8410 SampleList Window Extensions When a 460 66 controis an 8400 AuoSampler or 8410 Autainjector the Sortir tor tat etument contain tre lowing device dependent fie Many of ese Samples elda are on shown and available rei when t
29. a seated and hat We s Edit Standard Compoundis Open Compound par and Madly a Standard compound or create a lat ot new compounds Standard Compound isthe Standard compounds avaliable Cancet Close AutoTune ioa execuing a uncia Tune Flle Naming Conventions AutoTune automaticaly rames and saves ach ney ees tune He veing he Tolowing orat Compound Namo Mode Date created dac W atie by that name already existe the e name increments Example Second Negatve CI Tuning on October 8 2003 pake Vena CI 08 Oct 2003 dae Second ile FG 43 Neg C1 08 Oc 2003 eda Tune to Target Ratios Tune to Target alc a OCA ore tar lian e nexo Tai pte uot ale on c Pdl iere Exanles we fe lon sasa egrets orum EPA methods e Ryo GER GHI far COM bar soin There dia he aset tothe base pe ol 31a cabanas QE parce daa ur war i e mas pec Ther ve a aed ret erenn o dnd npa rte sem epu CR Ca gei abe quera ut trot nasser Sica c Seti mata Cris pat ge o ai sumer ric pua Ea ana dosnt sont sni Bre ym el ux Pam necant fren Quy BPS a DET ae ubi aate aou wi e meg Rae Dot rng la prp lk one f he long ene paran Ga lc inc Use preso acaured ta Ne To nma he canon gas rte ety peren don 2 oet Tune to target ratios rm ee a i Sa na pany poem e e 3 dnt Tune to target ratioa window cick Tune Calibration Gas Ratios direct A Select the Tune Compound data tle Seca data tle at has on ratos that ormaly pas BETPP ana BFB EPA 8220 requ
30. an one Method Tere are two Ways to do ns change he acive Method nthe Sues or se a SequeneLi Changing the Method in the SampleList Change tne Method by acting a Method in a Saget row aem faa Eee Using the SequenceList Window one File menu select s New SequenceList or Open SequenceList eem Cres new Sequancelist or open one tom the System Conta toobar alga Torta SS RR ae Inthe Sequencel ist select an Aion Enter he Method and the SampleList or lek Browse t selec wom al Monitor Run Status Ater making an nection montor he rn in System Conto The status and conve window and he Toolbar dia he module status Message Log Automaton actors anderer are recorded in ta Message Log The most decent envy is at Ihe bottom Double isk ina status bar a the boton oi he tument window to display the entye Message Log The og i saved in mne Veram WS MSGLOG dreciory Each Message Log ay stamped wih he ime and date Automated MS Report Generation hd Rapor setans o your Methods to generate automated reports tom System Conti ater each injecton atr Recaeuaons or by ung Prnt tion in a Sequence window Vien Automated Pang i enable a hecknam appears in e tort o me Enable Automated Ping men To disable automated Report pining dureg an automated sequence t clos o Recaleviatons lek Enable Automated Printing rom fe ion mento abe n and remove a check mari ASC a S
31. ane ection do one o the folowing Cich Inject Single Sampie trom he inject menu Cickihe nec Singe Sample icon on the System Contrai menu bar a ex eee aeg memo y ekg inject Single Sample trom me Inject menu apens he toboning windon The tous are dependent on e samping device For more eration about hve ls soo ha SompleLst and Rocks Fide secon nthe MS Data Handing Users Guido part rumbor 3954038 Lc Rex eie SPI A Enter the Data File Name and Path Data Fle names can have up o 285 charts They can be a combination ot Samp 10 jection date modue name and reckon number Click Data Fles o open the Data Fles Generaton window Selec the dive leter and surety pathi to store your les om he leftside The default deco is he data sundeeco o our VeranivS decry Creste a tenane tomat on he right side You can comaine a tx ent with the Sir mios 1o spect lenames wit varatle sample njecton iniormaton An example f he enam upsets as you eter Hename apeateaon Select a RecalcList The ties me Sampietis creates can bo stored In a Recalctist which vadis the ames at th crested fes and oiner parameters You can reprocass a to reprint he repr or to procese daa wih lek RelealeLiat 1o open he RelcalcList Generation window You can create a Tew RecaeLs append io an enistng RecacLs ovrwite a Recast or not lene or update a Recalt tata test pem P tamtam mima netti gee a MB E o Cl
32. ay and Saved toctvaram methods drecn See he along Tune Stability For opm tune stabilty he temperature of he mass analyzer must bo sable and a e set po temperature lhe mano betore you at AloTuno This my take several hours ne instrument as not been under vacuum for ng The ang compound signal mus be stable betore starting AutoTune Cal Gas onor atleast To routes or PPG anal til NOTET rar nace o ure ie Patna dar Vers ra ip aE f signat me oo soute maybe conamine Clea he on source and Racine Turing i ot required atter changing or replacing anion volume however tuning he instrument ater cleaning the on source i recommended This ptmzes the sumen and ensures eie Instrument operating pope AutoTune Options ek AutoTune lon ie Quad cob Select e Analyzer and Polaty 10 ne andor cata Tune and Calibrate Corpiet optimization ot me mass analyzer or ensi esolaon and mass alban Ser e mas esaluion wih a peak wath of 07 amu na peak hah A reportis generated win a graphie ot ha peak shapes and interes saved ith mode name and date and a texi report wih Gealed mass accuracy and Calibrate Update e loaded tune tie by re turing mase rescuon and se alain me mast No changes are made ta setting in the lon source orte Soranalyeng quadrupole A new tle rare and report are created and saved Report Create and save a tune repor Compare wih previous reports to Seiermine I retuning i required
33. be eded AutoSampler Window The AutoSampler window has parameters or ho 84008410 AvtoSampler The parameters incudo tho carping syringe s the pensation dep lor sample solvent wasn vias he eta clean and clear modes and Pe econ modes Default clean steps are used automaton stopped or a serous fault is detecto Select Pre and Pest incon syringe cean modas EE coperta ae EP cd juin pm e emen ait EH Injection Mode There are seven injection modes Standard SH Sae Standard On Column Neat Viscous and Volle User Delned and SPME Sea ap for a dealer doscipton t al ection lode parameters Santor z panne pea L ac Auto Configuring the Method though you ea 450 GC Method while te corresponding GC lor in System Cont you are wamad the Method cantgurabon doesnot malen he taninar oo ne GC mm PN Ciok Yes 1o change he Mead to maich he GC Ch No to acopt the Meta 1 you autocotre te Method Terang lo spayed Uploading the Method from the 450 GC Uploading he method tom the GC and saving tin MS Workstation allows you ta eat me local Method on o 450 GC Rot panel and save ton he Wonton To upload a 450 0 Method to System Conto lck Upload Active Method tom Modules ron 1e File mens You are prompted othe name at fe tle 1o save the Method The ave Method System Corti updates wth 480 GC parameter The Startup Method W
34. ck Overwite the Recale it each time the Samplelist Begins 1o overt ian lek Append to an existing Baap 1o append a number 1o e new SEE QuickStart QuckStar injects a singe sample without using System Conta drecty Customize QuickStart to set up instruments tor routine use See onine heb tor QuekStan stans System Cantal and walts tor ali modules to og in betore opening xeesadws na lt Enterinfmaton in the MS OuiekStart window about your ien For more tomaton about e ids soe e Samoa ist and Reset Fld secto rne MS Data Handing sors Guido part number 934038 Hoare ain NEM EZ eo Autosampler Display Each of he supporte autosamples has a graphie representation fhe arousal or ay n System Canal cack he auteamaer Ennan S rr pent e n vali doi c on e prie Sal in e display Ater the inject Single Sample dialog apens enter te arata and male ine cion Running Priority Samples Using Inject Single Sample Use ether ie graphical vial selection or ect Single Sample 10 make a priorty jeciondunng auomated analyses Whio automaton ie nunning cic Suspend on the current active Samples Alomition e suspended aer e curent run s competed 2 pense tne viai postion of the priorty sample ar choose Inet singe sample Aor comple he pray nun cick Resume o re start te SampleLs LC MS or GC MS Injecting Multiple Samples You can create SenpleLsts for diferent autosamplers and use more an
35. contains the Intl Spit State and Soit Ratio tor he 1079 injector Tra 1078 salate To ha seting wnan e method e activated and s restore ahs seting hen each chromatographie run i completed The folowing rows contain me ime programmed setings tor the 1079 Spit Fata The Spit Vae a smashed oa aio each row atthe C Tie a typical program for a apes injection A ow spit rao conserves camer gas whe keeping conameans trom bulding upin tre inyectar The iecoris kept spiess rabo 1 minute so all ot Pe anales are focused on ecnune se a spit rao o 100 afer injection 1 flush residual solvent rom re nectar ne smite Sine 8h T rr j L3 7 J j E cem 1177 Injector The 1177 injector window has controls tor tne Injector Oven and e Spit Ratio ethene 2 uem Ya Cu paman PM NOTE The EFS ype me covesaonain potion he ow Presa Sesion ust be congue betore the Spit Rabo can be programmed Speciali to ud an 1177 St Rate Program a Front 1177 must have a conesponding Froni Type 21 EFC a Made 1177 must aveacoreeponding Whale Type 21 EFC ana Rear 77 must have a conesponding Ram Type 21 EFC Use he Spit Rao spreadsheet to buks a Spit Ratio time program to conval he 1177 mec Type 21 EFC comeiaton The test row of he spreadsheet contains the nal Spit State and Spit Ratio tor e 1177 Injector Me 1177 aculbrates o is sete when te method s ee and s estore 1a 1a seing when each chromaloprame run i
36. e For ttis addinal precursor ion select Auto ind produet masses or eet the product masses To ada ons an colin vatages to an existing method select Append and the appropriate method 10 Analog Channels Two analog channels can be cortigured in e 300 Serias using Analog Out elects or by deining PML cups To use Analog Out othe folowing 2 Select Analog output of trom the Tools Menu 2 The Analog Out widow opens enag Pope E fee DE T n Hatred et 3 7 e O50 E erm I Rare 10 gt Connect the wires o he pins on the User VO consecr on the back of he instrument Far example he preceding sereen Ang cuu P1 the ip mre connects opin T4 and ne ground connects on 32 Check In Use tor he appropriate channel in he Analog Out dialog The part funeri te user VO Board is CUBOS 0047 105 Automation File Editor Overview Use he Autormaton Fle Etro create and edi Samples RecaleLlsts and Sequences outside one MS Wonstaton System Contr appeaton Races ta ot ane Automaton Pia Ector ino drupg automated s ROTE SsmgUSS and Sequences a are GC and running IW Siam onto ese be accessed ma one Automation Fle Ear Accessing the Automation File Editor Clk Automation Fle Editor buston EE on tne Wotton Tra Create or Edit a RecalcList Select a Now Racalclist or Open an sing Recast tom he Fle menu The Recast window opons The Recalcl at may cortain auosamper ad sa
37. e not late Fea Description rase Tre Ste nume a te secondi sample injected This coun is rise ntan me T ir nara am Er Een UL eife Ree HT T RE E ET E EH be aon gs schen voume eal E tanp sereen ege te Sarena cid piste coi ha ave been ran Faa Description nesonaite cante pic iode oe recton ot te scar sampe Dua Mose When be Surplus i amp pls en ea see Erase tgo te Sargent a dioe pets ne cepi a have bon Rem EET Um nemen Werte aues LY T ie ely e etr delay ra tlre th sample is ete Decrement cay neon 8400 8410 Carousel Display When an 8400 AuoSampleror B410 Aue le connected to the 480 06 e Carousel dapiay ia veine n te 480 0 Siue and Cantor window 100 AutoSampler Cause 410 Alyse Carouse em Beseipion Be paese Pe nf ace Salt a er action Deseripion open re Gre Samp og OHS pay ive Sanaa Waa 431 GC System Control Command Reference Status and Control Window The 431 GC Status and Cont window haste display areas he S160 Open asia Te Temperate Zares us daly Pe her EFC Situs display and No b00 410 Carousel d T krmit dista Vo 49120 Opusio dep we as formation abut 1e curent un and Ine overas tae P Tdi e ta dei atr an o forget re Sis deni whlch has Pa setpoint and curent actual temperate ol each GC Under e te
38. e referred t as 255 nthe compound ti t you procese a compound tabe a specs a iss rnae tne fofowing message logged vali sean funclon Cannot Quatate The fooweg shows he part ol he Method Builder window that has Scan Chamel Vaaton Enabled AP ien Merc pee uM Scan Time Set me scan speed o he MS The scan speed s he amount alma he analyzing quadrupole does the sean Fora scan or MSIS scan experiments fete scan tme t 0 5 seconds Mare dala ponts are acqured wih hor Sean Tenes Inveasing te scan ne Increases Ine signal 1o nice ralo since he quadrupole spends mare me on io parca an For St SAM andi HRW cecreaso ho scan ime allows more areitons t be scanned arrose a ena Collision Cell Gas Enable tum the CID ges such as Argon on for SIMS operations Feo Peak Width Sete peak with or Quadruple 1 Q1 or Quadrupole 3 a Cleat he value trom the most recent AutoTune e he default seting and S appropiate tor mast aplcatons Scan SIM For SRM and MRM reactors in MSIVS mode the pak with of Qt can be teased while keeping Q3 calixated or smaller Te signal increases by Slowing Gt fo wars a wider peak wath while Q3 detects te product spectra ia nartomer pesi Mass List Enter tne desred mass range Ful Scan single masses SIM nd precursor fd produc masses WSN Q i used tor pe quadrupole noen oniy lon Polarity lk the on Polarty erty to such between po
39. e used ou dan incide a signature Ine For more data tete t reno help See out Warton on page 10 E System Control Toolbar The systern contol toolbar is near he ta athe screen Seg Snn te trier Y vua vien The tosowog describes the fnclons zi G S ars ton El Saan nas Saigin Y viento pir a Nee ll osos Ej as B opr eee E TES Quad Status and Control Window Check he status af te instrument components monitor data segona and procese data using e Quad Status and Cori window Create your peer indo contain luis Busen BEER RRR LKS 2 L SIL lal wam AIRS ci Quadrupole Module Menu File Open ar print a tie Method Eat me curent active Method using Method Bulder Conliguraion Synchronizaton signa setings and MS Opens settings aa SS Syme Signal Enate or dable me Start and Ready signals for external devices Ta enabie a Syne Signal operation connect a cable Irom the device o ha Select be Enabled check box to activate te desired unen M Cick Wiring to view a wing anan MS Options Haare agnosie tooi View and et S Options vane iren software setings Chack marka 7 indicate a Hardware mach a X indicates a Hardware cont an X is present call your Varian representatie Tooks Access MS Tools auch as MS MS Breakdown Pri Edter Plat ie a Bakeou system and Overnight Standby ma
40. ed in Qum O3 Prst Mass The mass tor CA m2 Q8 Last Mass Used oniy i a mass ranges 1o be passed in OS Capita Vots Coliion Energy Vots Duell Factor Baar tactor tor seting det ies The dealt Requested Duel Tene he Dwet Time is muti by the Du Factor foreach vanaiton Requested Scan Time th Duell Ture R based onthe umber ot raneilons nthe segment and amp multe by me Dwel Factor foreach varelion The range s 0 00610 T4 Seconda nm Relenton tne Minutes n decimal minutes RT Window Retanton me window in decimal minutes 7 Compete the spreadsheet win he information fr your compounds using he MEM parameter n ho MRM drabase or another Source sch as an applcton ot commercio pens rabasa Be corte me regure tels 8 Cick Save As nd than save he spreadsheet wih a new nane Importing MRIs to Scan Method To importa completed MRM spreadsheet 1o me Scan Method do he allowing 1 From the Schede MRM window lek Browse in 1e Inport MR to Sean Maina sea gt In te ingot tie name window locate the spreadsheet and then ack Open 30 Select ener Requested scan me or Requested dwell ime and ener he 4 Enter the Mein segment me wath in minutes This determines the Shortest tne wih hat MRM uses to opine te metroa By using a male une nde IMAM breaks he mathas int more segments gt Enter the Maximum number o segments Ts the maximum number at Segmente MRM uses to opimze ne method The maximum aceepable proni S
41. erview Use imed MAM vansltone MRM vaneions to automaticaly break up an Sequslton method no mutipie segments The use of may segments inan Scqusiton method slone lor longer MRM awes ires and thereto increases Before you use IMAM enable he Scan Channel Vaidaton option in me Valdaton ab one MS Dara Review Preferences General dalog You can schedule timed MAN antes by tling in a lak argas or by poring ma MAM data om an exstag method The osowing descbes both To create timed MRM ransitons lom Metod Bulder or System Cota Method Builder selec Acquisition Method aoa then cio Schedule MRM i open the Schedule RM ino To create med MRM ranstens trom he System Conti Quad Mode ce Define Sean method con When the window opens click the Schedule MRM buton he lower iett The Schedule MRM window has te flowing four areas Create MRM spreadsheet template Greate a tab delimited t ie to uae a a empate n Meroant Excel io enter tne MRM ransiions Exporta Scan Method to a MRM spreadsheet Esport an easing method mh a tab na n impon MANE to Scan Method mpon a tex tle T wh MRM data trom Microsoft Excel iio Method Buter MRO Import Statistics Summary normaton about the last MRM inport acion reang WOTE ATer s eTo asan ON Take aa Changer o We TAT Me and import again vit MRM petimus mm Te m I em M e Ca ro Clck Hep open a window that outines how to create l2
42. es Generation window Select the path and The e name forthe aata fe Select the data tle directory Pom the et side Create a tle name specifeation on ho right Contin eet erty wh o 5 vail symbols 1o spect le name ih sample njecion spec ntormanon An example ne ie name i updated Sou eter e e namne speciteaton Specifying a RecalcList To select the desired Roca option lckRiecleList The Benn Generaon window opena Yau can cate a new ReclcUst append i an isting Recalls or nl create hor update Recast When you select rate and update a new cas e automatcal genoraed Recacar dos nl overarne an exei PacalcLit a Recalls win the same tle name et 1e wy created ReeacLst has a rumba appended tte nare a mika unique ano preveni the older calcei om Being venwton TEE as D Changing Default SampleList Entries When you add a new row to a Samples dell values are use or each em To change he detauit values 7 Cikk Default inne open Samples window gt Ener the desired dtt values and click Save Using More Than One Method for Injections You can do automated injections using more han one Method by changing the active Mead om e Samples or uning a Sequence Changing the Method in the SampleList Change he Method used during injections by activating a Method in a Samplet ss row Bin
43. ew setup Help Onine Heip and e About window Quadrupole Toolbar choose Fie to Display Open and display data ties and ed headers IE stan cotecung Data Acure aara rom oriy tne sto Sees and not ather ontiured odes Calle daa using e curent instrament conditions Ensure hate source and deir are an olor you use hi Tenure Sl Pint ne wow screen Turn Detector On and of Contra he tment or discharge vorage accelerator and electron mtl The coro We con eaten he falowng Gayor aon Red flamen broken Yelow System in process ot tuming on or ls wating tor example a amen delay ime Y t tum yellow ana hen tame gray aat sale has prevented he lamen and me mulier rom ning o EB set strument Parameters Set on source and ane parameters and iip paints to protect the decranc and vacuum shut ont Tabbed ven est Sherine GC or LC contguraton VC Source GC only Tum on and ott callraton and CI gases and ie ament Set amont curent dectargo vonage and electron energy and set the Source and Transfer Line temperare RS amem ma oe m e APL Source LC On Select API on af sequence N2 Hg ime and Gus pressure abode Select Neuling gas pe and set the AP Housing Drying and Vaporizer gas temperatures For normal operation de ne API on soduence seti Automate ER EET pei Sale hore are vee sectors and oriy Varian Representatives can change Hah voltage enable and on Gauge modes Top po
44. ge reum Some keywords create text Ike o printing statements using and GET FILENAME uB FORMULA ue NAME st_To_sTRING SAMPLE NAME STRING RESTORE Sue Fie name VERSION ome keywords re toute me text created by pining statements Ug and o oer places than na dipl DACS RESTORE fla name CR pacs save Mie name CR FLEAPPEND ewe CR FILENAME ta name CR me ore fia name CR IONS RESTORE itle name CR ou sve fia name CR ico message CR uB FIND compound name CR E UST RESTORE he name CR usr save Mie name CR msosox Matei eat CR elut CR Macro CR PCT LABEL taal teat CR Pcr Ime uet CR STRING SAVE eo CR fen user svings STRING TO UST een Sus name ext sc 6 Statements may include comments A ends the statement and starts he comment egy DETECTOR 10 8 rae the detector 1o make ie pesk ope 7 AI numerica values retared ton PAW Macros are real numbers Usua Saera synas alowed eg iy e contis 00 month aayi x When parameters are passed to macros they are referred o within the macro as ut 62 1A sod and 5 6 Fe macro TIMES s deed as PPS times 312 aquala 021 42 CR and you pe TMES 79 andthe instrument wi pe out mes 9 equas 63 NOTE St Si can De used as local variables win any macra 3 When passing parameters to a macro or keyword you can leave out pareriheses at he top evel Al these are val statements raus vans manas anas ranna ara a value to a variatie or paramete
45. he B400 Autsampler or B410 Aurairyecior used in Dupicate Mode of Dual Mode See neip on ne gonere SampleLit Window tora desrplon ome leis Foa Description lets Turm Ee ae person n Fre ie arain tr me ned cond sangis w played wen a 2 jection i Avanes or Dean EEG MISSA 2 er 80 Alnor Serta Wines Sneed o Cis itai iiie fa Gu de ara ha val SO Pectin une ch nn nile Wen ne Sarees eed in Syste Carro he maumum wees he sg rci Cri arg agra ne mpi volame pus set vate Slt alte secede pace tat ha aroas ben ra the econ volame in miter t r e secon Factor fe anter pic iode or De jon o te soar sump bua E tne sree epe cmn T Gag aparaton he sane volume pus solvent lame eren pe neo bypass me samples Speed are a Be ie mantge to reeds Bus meae ae 1 Stet eae yeu cheese Dupin ocean rss Tg Ta ae Sn dy tmnt icin at seen suns er aye ure cons pa in e message iog 8400 8410 Carousel Display When an 8400 AuioSampler or 8410 Autoinjector ie connected othe 450 6 e Carousel aplay s shown in me 450 00 Staus and Cono window 100 AutSampler Cause B410 Atoinjactor Carouse em Desc Be rset van Fe ace Soleil were carie action Desc IT PML Overview of PML The procedures at generate inan rearing resulta trom e mass ipeesometrar wien n a la
46. hen System Corio is started he tast actva Method ls activated and the intial seringa nthe Method me setate To change Methods cick Activate a Method o ihe System Control Toolbar Editing 450 GC Methods kn tho buton In Pe 48 0 Sut and Conto Window to open e ale Mood Any Method secon can be oi Atar editing the Method and casing the Method Bur windo you are rompted a reactivate me Melod Resctvang ne Method downloads he anges to 1e Mode oona E 431 GC Methods 431 GC Windows lek 431 6 in Method Bur K arat he Mode Control Parameters By hangng he made adres nm parameter window a method developed lor n instument using one mode adresa canbe easly madd Tor another Paiument ata dierent module adress The 431 OC Method section has two windows GC Conal aans Exch window has pe 431 GC Control The GG Control window has sectans tor column flow injector aven and clue metera hat conta he GC doing a chromatographic un Constant Column Flow Mode Select Constant Flaw Ot o show the spreadsheet The test ow ot the spreadsheet contains the nll pressure and hold tie The acumen equibate ois seting wren the method i seivated and s restored sting after each cvomalogragne runs complted The foloweg rs contain me programmed stings tor each ramo segment Esch segment ramps to he speed pressure at the spei rate assuming erae i achevati and Wen nott Ie pressure or Ie spaced re
47. ial Spocteaton and AubMx Clk Add Lines oad several Ines to the SampieLt From the ProStar 410 Sample ist Press Begin and you are prompted to select 2 Memed Uee e ative Metod inat was or cic Browse use another lek OK to begin the run LC MS ProStar 420 and 430 SampleList The ProStar 20 and Prostar 430 SamgleLists have genre System Control Sample Vas and his Tor operations specter cach AvioSamaer To buld a ProStar 420 Sampiel ist or a ProStar 430 SampleList tok Ada You can enter atomaton common oc ali samples You can select tne number at etis to be added sequel 1o tha Sample sst Weh te Prosar 420 you an sect e at and last vale to be aed 10 he Samples win he Sesurement ma he frt ana last vial nares at wih e same Tray Type iener A 8 C or D Wit the ProStar 430 you can spec he es and last Row and Column to be dod You can aiso Selo to ii by Rows or Columns which determines The ate ls processed Yom le t igh by row oom op i bontom by coms Solo Partal Loop ti Ful Loop or ul Pickup trom he Inecton Modo teid S lo the User Program tom Ms eid also Several heids are abled when User Programs seecod Fo the ProStar 430 use he Plata colum ospecly he plate at has he weli or vil wih the sample Enter a pate number between T This led s doable lees he Prostar 430 s equpped wih te Plata Feeder optan The ProStar 420 WelVa feld species he sample location as a combination of Tow later and clue n
48. id Select e intemal standard tor a pariclar target compound Tom the lo Use Identify Group Members Sometimes target compounds are dented as a member at a group ot compounds iot example Trhatometnanes THM liy aromas et Enter te deste name in the Group Names tieid more than one Group s designated in a method select the Group Name trom ela Quan lons lek the Quan tons tab in he Compound Tatie edtor When peaks are added othe Compound Tabie n sofware names a Base Peak oet tense lon Re Guantaton on The piat shows te mass comatogam for the chosen Quan ion Edit Quan lons Selecta erent Ouan ion a sum of ions or a range of ons E you selected lon Select ne 10 Pot opens Select Show Format and Examples 1o payne ways 1o specly one Tre Pots redrawn automaticaly ery sc fhe Qua ans You ca determine he chosen Quam ore teat conl ornare are Interterences due fo to eun peaks or i WGTE TRs Sean Paa Chances an ny eva or WES s alysis because ouantatonis done oir on igual Channels ol ormalon mm CE xx Ea pee ee Edit Retention Time To change the retention ime ck the desired sean in me Chromatogram dac Look at Other Compounds Clck Next or Previous 1o see the Quan fon itrmatn tor ather peaksin the Compound Tabie Select Qualiter lons haen t specitying quantitation ons you can slat quale Cle Load o ener automate he tvoo mea lent culte ca cand
49. injections in this test eid premens mab Ad a Command or Other parameters tor post rua even Injection Mode Select No Injection Parti Loopil Full Loop or pl Plekup trom e mana In Volume Te 4801 C comes wih a 100 us mise with pL increments Tray Create a samples tor ether o Lett or Right vay Vial Enter a combination of a row leter ana a coumo number The ilb ows and column numbers depend on the ype of pate contr M meena At rough me mne A though e Wash Volume Enter to 9 washes Automix Routines Use thes routines only with he 4 3 Adapter Tray ve loop Injections can be GC MS CP 8400 8410 SampleList In addtion tthe Generic Samples fontes tne CP B400 410 has setings Tor vial poston inyectan Volume and injector Selecion 1 SPME hardware 8 tale the icon Volume ez dested sad ne Iber Norton Tire set rne GC conv section of th Mead a used Tor sample eactn Selec Manual tom the injectors Used teid to cable he CP B400410 and fake a mana nectan Inthe otowing example 1e RES Samples 1 and 2 hen wars tor a manua ocior om the GC or sample Sand conis wih samples 4 and S using the CP BADOBAYO The pagan can make two injections in the same GC method run Select Second injector om ne iecore Usea tld M two inctrs are used you can do the folowing wih me second ect ect ho same sample aa e ral jecion Duplcate or nec the sample
50. ints of pe source pressure mano pressure and turbo speed Status athe POB Protect Swech HE betine Sean Method Se cureny active scan parameters tor Qt and Q3 Sequin of cer or role dat ond detection e postv or negatis ona Faler io Acquistion Mood Window tor more nation AE ea i mem EE Mc E sca BB Auotune Auiomateay unes ences Q1 and 03 and cleats ha mass arse Foretoematon on e Au Tune proces reler t0 Aubune Furetana and pons Module Views Sr er TY CHE The tve deta vows are View t Displays Quad haan satus Prot Canta and etan indo View N Displays Quad Instrument status Cromatoram Specva and Cerro widow View IN Displays Quad Inerument status Chromatogram Spectra and Prete E View IV Displays Quad Instrument status Chromalagran Spectra and Lary View V Displays Quad Instrument status Chromatogr List and Picture Eal select views Eat mo tve preset ews Select and pla Views nth Guad window snute am between dferen arrangements ard save Them as Iki N or V Reset he settage wah the Dolau buton Profle Real tne ploto the dl sgnal acquired over te mass ranges scanned protte data Spectra Display acquisiton ot eler pie or envoi specva tom the chromatograms Lia Program or temporary storage af ists of numbers tar manipulation or ated analysis by Paw macro commande Centold Rea tme ba graph display of temas peaks conto o ha
51. iz Q3 Fst Mass The masa tor O3 mz C03 Last Mass Used ony It a mass range is to be passed in C3 m z Capilary Vots Colson Energy Vots M Dwet Factor Relative acrior seting duel times The defaut ie 1 Requested Dwell Tane the Dwel Tire is mutipled by the Dwell Fact Tor each ransion Requested Scan Tie the Dwal Time is based on e number e segment and is maga n te Duel Factor for each ranstn The range amp 0 008 0 14 seconds Retention tme Minutes in decimal minutes For example 1 minute and Sp second s 150 mites RT Window Renton Tene Window in decimal minutes mn N E RR RS amp Complete the sread heet wih the norat or our compound using he MEM parameter i the MRM database or another Source such as an applet voie conne or persona dates Be su o comete Me Kae 7 Use me Save As command to save e spreadsheet wih a new name Keep e sds Te format Importing MRMs to Scan Method Ta import a completed MMs spreadsheet othe scan method do te flog From the Schede ERM window clek Browse in the Import MAMS to Sean Meinod area The import We name window opes 2 In te Import fle name window locate the spreadsheet and inen sek Open Tre Te ame appears in me Sehedue MRM window gt Select Requested sca ie or Requested duel tne and erter te tme in 4 Enter the Mein segment te wath in minutes This determines the Shores ime wih hat MRM uaes a optime he otha By using a male tne warn UM breaks ne
52. le The User Program can da sample handing and mecion acions When te User Program is selected as the Inaction Mode the folowing fs S necnon Volume Wash Volume and Automix Routines The User Program steps sre used ead o e method parameters You must use he User Program ithe Tom syringe a selected The olowing four shows se he User Program Actons Far sac spected action there coua be severa pions auch as Poston Amounts Speed Soo and Hegre Siart e run by slecing Start run on te INJECT Marker or seieciing Start run at End of User Program LC MS 460 LC SampleList To create a 460 0 SompieLiat do he folowing 7 Ciek Edit Automation Files t ope Te Automation Fe Editor 2 Cick New Automation Flle ard cick new SampleL st 30 Entra fle rame and cick Save gt An empty Samples wih your He rame opens re Add to add one sample Cick Add Lines to add several samples or a plate Cick Hardware to secl the vay ype or each positon sample lop Velum an nica m Syringe or Prep Mods s stalled rere Comet EN aree ESTA S enie Erg Sample Nome Enero cat nane oras samples Sample Type Selec Analysis Calibration Vericalion c Baseline ron the Cal Level This numerical tei used win Calibration and Verleaton Sample Types Inj Enter the number ot injections in tnis merca feld Injection Note Enter a note about me sample or the
53. les presen inthe Fterence Spec mmi Es puerum Clk Add o add the next most intense relrence specum iont The Qualis Tabie has he allowing eia donClckinthe on feld to see ne choices Ratio Shows me ers o he qualifier on as a percentage of the chosen Quan oni uet Alowed percentage deviation fom the Rao You can change he ela ot 05 The Uncertainty aliai range dependent on a lon Rato Type seting in he Calculations Setup Diieg Abeoite t ar Raves Lowes and Highs For ien 3 me top ne he previous example and me alowed range tor tne rao is B7 50 2 absolute Tode EF ar i pe Loue value and 10745 athe Hit value area As samples are processed Calbraon Analysis or Vair runs ha Sunita ln testa are done betore me pesk amp Integrated Calculations Tab Cek te Calculations tab 1o cepa te following il Bee mme E Enter Number of Calibration Levels and Amounts Erter the number ot calbraton levels anata aout the raan curve e elation amouris and vats unt tamea St serlo 3 pome e re E The pons intormatan is updated in he Compound Tabie This formation an be automatically entered far Ihe oiner compounds in etate amio skins ae TAD Irenku d TCLS RAET Urea igo 0 wes NLET een Havas Clck Copy Amounts o automatically enter the trst value or e ather ves Rees ths process tor all standarde n
54. mperature zone i the Oven Status Next ia the injector EFC Status spay which has oration about me injector 431 G0e wh an 400 AunoSampler or 8410 Autoirjector aa ha 84008410 Carousel The deply shows when viala are a be sampled which vals have oen sampled and which var being sampled 431 GC Operation Display The 431 GC Operation display is ne let porton ofthe 431 GC Stats and Coni window aen Bo rre Tem Description ont Meroe Surging T et Ready Tre siata sf Ine GE Theip agent Re GC ie Ranay ar Farag Orama ms p ee ro na jern in Soto Equa a resar Sipon Temperature Zones Status Display Sow m 8 NE Tempera zones Tere dahil temperatures ot tecum ayy be heated The day i Faull sau occurs of OM fa zone oven tthe zone was not coriurd by he 421 00 than NA e displayed re Serpoiris and actua temgerdure Clok a temperature zone ey 0 ply the arespordng component sia n the Companont Status Display The LEDs indicate the status o each temperature zone the zone ia not ante or me zone oven s of Ie LED say ha temperature zone ie Not Rendy or ter i a faut he LED red thee me LED t green Injector EFC Status Display mme FF Yoo Pentet Yo ieee ne T ST ae e 5 e Description mll TE Sunn sng and eral are aiai m Serr re i000 Dio m cert sla ote 1177 ica mabe The apit ne cede Caur Py h Po
55. mple specie data nanging intrmaton To Recast conta the Data Fle Recale Noles Ener notes about te Recalculation one Data Fle AutoLink Enter post calculatan operations Report Generate a report tor te selected data tle Note Pata data tle le net ghed n tne Data Fe cmn no report i generated Actions Ater generating a report using e Report button clok Actions t show e possbie acions P Create or Edit a SampleList To create or edit a SampleList do he folowing i From the Fle menu select New t creste a new Samples or select Open oopen an ersing ampieList o ea 107 gt Select folder forthe Samples type a name and cic Save mes NE seme Freer ferie 3 Select te autosampler and ciek OK 4 Se the Spreadsheet conn by dragging heir bordera wth the let mouse utor nt cicking a column header depey fing apt The Sample Name cota does not scs SampleList Fields Sample Name Enter me sample name Csck Data Fies 1o enter sample names only Se is adn me Data Pa he sample name iaa you entered It Sample Type Use he sco bar to see all aer en Ca ox er Cul Level Designate me abor level ora Cabral sample You ean nier up to ten ives Ini Specii he rumbor ot injections af he sample You may make upto nine epee recta or each sampe Injection Notes Enter notes about ihe semple Ing AutoLinkc Reter to Inject Single Sample Menu on page 14 DE du Aaa te oe ay am
56. n Column Flow mode disables the spreadsheet and enatis me Constant Pow rate eid amena 7 HL DNE I a aia When he method is activated n System Contrai MS Workstation downioade the pproprate pressure program corespondng 1o he Column Temperature rogram ma Column Setup Langin ana Diameter the Carrer Gas nd he rien Barone Pressure These parameters ae spected on be 4503C Tant pane and me Baramevi Presse measured y me 450 G6 wren the Memod s Ee The 152 00 runs he pressure program t hoe Column Flow at he spcild vl as he Column Temperature ramped during Column Oven Window se he Goluma Oven Program t spocfy e Coolant Parameters and Stablzalen Time af he Calne Oven and Yo speci a programmatie temperature ramp Tum he Coolant or the Oven on or ot Set te Coolant temperature and Coolant Timeout in minutes E temperature ramp program to heat ando con te Column Oven The tst ow of he spreadsheet has he intial temperature and hold time forthe Column Oven When tre metrad s activated m Column Oven equlraer i is setting nd stabilizes Tor he sped Slabiizatan Time Ater each Chromatographie run t completed he temperature i restored o IN stg The varaen contain me programmed setings tor each ot me ramp segmente acn segment rampe othe specited onpataur at n specta fale assuming ne ate cheval and en hod to lomgeralre forme pected ine TOTE The Rate fine sr ways Bank ard cael be Eid AS the Total te cannot
57. ng and iiaa dant TCD Status nem Description epis ttim ra TCD deter component has occured spe te TCD Econ seting in the active metroa Displays ie surent TOD Range seting Delays the electrometer ione constant seting fom the TCD Dire ike sive e epis te TCO Flamen Temperat seting in he seh Euer eps mA te aad TOD aaa Reer aaye S Pe TGD brizas Balance On Ra 450 GU ae ray lye unen TCO Poy sening Pone TEQUE Fa G FID Status nem Description edt a taut in te FID detector component has cured aza satiro in e ce meroa apa e FIO Rangs irita onation sting ar me tme io th ejecrometer im corsant seting fom the FID Beea Sarai Damay i nV al FD desar SG T OSE ens Danay he eal easan nonek a Fra G ECD Status TSD Status nem Descripton Rem Bescripton PFPD Status nem Desciplon eyed aut in Pe PFPD detector component occured eire PFPD Rara seting inte ate mela Begley PFPD Sere Rec Mode sening in the aive E Interactive Scaling fhe folowing acions can be done in the chromatogram chsplay windon Etecro Acton ere We area oi meresi an a lay Whenever he dap The 450 00 Se
58. nguage cad Paw Macro Language PML thas omvrands hat coro e Instrument or dia processing Users can we S o customize dat processing Simple Commands Since commands can be entre in ha Cin teid Typing tete quotes included nthe CTRL text box ps Hel i the Out Status spay on o task bar CANCEL stops the Paw macro running at the top leve OUT stops al paw ras you use e Quirke word pe scan to restart e PML Syntax The macros are text tles with he extension pmi There are macros in the PML Directory derat Caan 2008s PNL T you rete you owm macros or make new versions ol the esting anes stre Pham in e User PML Dreciory etaa C Veram User PNL Macros can be edted using te Pi Editor The names can contain letters numbers or underines but they must begin wi Run a macro by typing the name of the command ines of the instrument window CTRL or including the name In anote macro You can send arguments toa Paw Macros Gonsistot Examples Statements ts Heo onthe ey The Statements Reter To eem og m Statements are separated om each other by colons semcolos ne Coratora statements must have a matching END statement ey amp x Tn eee ae end tx 3 502 008 ie crepe 100 on k et e 1 zend END statements at the botom of a PAW Macro can be deleted Printing statements using and 7 can be stung together They ae terminated win me keyword CR cara
59. niions to automaticaly break up an aequo method nio muliple segments Using many segments inan qusan method alone ranger MRM des mee and tata nereases he senativey See haSchodulng Timed MAM Transitore scion on page 72 for more toman Setting up MS and MS MS Acquisitions Overview MS Operations Une 1 Sedi lon Monitoring SM at 250 ne 2 Ful Scan trom 100 10300 MSMS Operations ne 1 Seecied Reaction Mentoring SRM Lire 2 Precursor Sean Lre 3 Neural Loss Sean Line 4 Product on Sean MS Operations Full Sean Acquisition Scan tor al ions over a defined mass range Enter te Exampie e Bagan tom mass 13010280 on Mentoring SIM Detect ane or severa lons This mode is mare ative man Ful Sean Example ne 1 Negative SIM 221 Une 2 Negative SIM 414 Mied Sean Operations Ace data in Ful Scan and SIM modo athe same Une 1 Pepa Skt 120 Line 2 Potive SIM 220 Une 3 Potive Ful Scan rom 200 o 400 Mixed Polarity Operations Acro cata in postive and nogatv lon modes tor ESI and APCE Example Unei Poste SIM 120 Une 2 Postve Scan om 200 o 400 Ure 3 Negatve SIM 230 ies Nogatve SIM 290 ipu em za Multiple Time Windows Several time window
60. ny open automation screen indos o access windows pahin me automaton windows Arrange leona Arrange eusing icons in a row at th botom of e System onal window SequenceList Display the acive Sequence SompleList Display the actve Samples Recolctis spay o acte Recast Instrument 1 Status View the status c he modules connected o the system Message Log View the event a forthe MS mode The iog error messages E modules Tne last secon of the Windows menu displays te atached modules and their 21224 Stopped aa Ready Quad No Ready Help Menu The Het manu has the allowing options m pem Help Toples To tind he on a topic do he viaua 1 Select Hep Topica to display the foto Cle an tem ia Contents to see he nine hap 2 Oiek the Index tab and ener he test ow ees of a word Cic the desired Selection and ciek D pem Venett tei peti H 3 Enter a key word or phrase Kaap topics Cick a topic and then cick Display pame ea ioe puce ees eer oan About System Control About has naan about he sofware version instalation isan and a istot ihe instrament moules instale bout System Conta pone the came Mindon es does e About Workstaton Toolbar The flowing ean example Validate Installed Files Uses checksums oes and document errors Print a depot documenting ie fe system std he success calle of o test he iie pied and tested me 1O che person logged n logi IDs ar
61. ompound List Standard Compounds forthe selected mode Default tor thie Mode Set displayed Standard Compound ash dau New Add a nen lst of Standar Compounds Remove Remove a Standard Compound Comments Enter formation about tre Standard Compound lon Source Seect he lon source EI CI ESI or APC sein Tune Select which ions to use tor Tune or papa These lons are pinta Use in Report Select ions for th tune report Oniy the selected ions are reporte Exact miz Erter the exact mz ot each lon in e Sander compound Nao ot Isotopes Enter te umber at isotopes for me ion In the tune repor LC MS ESI Example In the AutoTune window select ppg from the Standard Compounds lat 2 Clek Ealt Standard Compoundis TH xT Set Capilary LCMS mode ony For standard compounds tat you create t he bors checked set me capilary vonage Tar each lon to he valve in Capilary vate held nor cheeked or Blank 1e vonage Pom ne capiary tune able te Capllary Vols LCMS mode oniy he Se Cagilary boxe checked you an set the toplryvotage a be used tor each une lm ethers ths optan s Tot avaiable I huning win PPO select Se Capt and use he deta capt votage EI Method Builder Using Method Builder Cek Method Builder inthe MS WorkStaton Toolbar TC Eas TS Edit an Existing Method 7 Cick Open an Existing Method File an hen cix OK Penaia 2 Select ne mobod by doing one of he toto
62. on fo rule Goons toa ull vetwoad ab wm a vital unimted number of GCs and Wodiaboes Eten ars 1o he cables and interface cards mat connect devices toe etr you are connecting your Gc t an existing Ethernet nemak you oed 1o know which ye o cabe to use Rear ta me Communatons secon ot Your GC Operators Manual The 400 Series GCs uses TCP Transmission Conti Protocol mana Protocol to communicate over he Ememet network The Workstaton PC and sach GC needs aunque IP adress ne addresses Ia are used o deny sch networked TCPI ave 450 GC System Control Command Reference Status and Control Window The 450 00 Status and Conti window laste dispiay areas 450 00 Gporaton aaplay he Temperate Zones Staus deploy tha Component Stas depla he S400 410 Cause e and the Chomatogian display The lef mast ply atea is he 450 6 Operation dapi wich contains formation abut 1e carent run and ne overa state G he GC The display area othe right ot the 452 GC ie he Temperature Zones epi whlch shows ho setpoint and current acu temperature of each heated Bone in ie GC Noi the Component Stats display Select me component or wich you wish Yo see more deal H cung ready and faults for 1e Selected component You can change the component tem deplsyed using e Selecton tox below ine display es or by icing on e coesponded Temperature zone ent n he temperatur zone lta dca pare mat have an 8400 AutoSampior or 8410 Autainjector installed wi have c
63. opens EUN moe M Varian Pics are tore in varias Ras eoan User created PL cr modited tactory PML are stored in ivaranwsl User areca The Situs Bar of he PML Editor displaya the oe namber of he curent cursor positon eee en te jnaHs sm g 001 DONE eher PML s and e Geode ore now sting be Code nl thom invoke s abort gm Sc QUI 2 stop the procese Fri te oniy uted De CTR trend Kee a h gt rm usine The Toc menu has three menu tems Version History t he SERC pobet is enabied cick ttis to display ihe version history di mo currentiy open PML LI
64. or ion 414 Product lon 219 and Colision energy 10 Multiple Reaction Monitoring MAM Mlle reacton monitoring s set up the Elected reaction montoem bu slows many MSIS ansion beween Ot and Gao be monitored Set me Requeeted Dwel Tane t ao or li montareg Example Line 1 Passive SIM Precursor 452 Product 225 Le 2 Passive SBA Precursor 525 Produet 315 Line 3 Posse SIM Precursor 633 Produet 380 Mixed Polarity MS MS Operations Acquistion of MRM in posiive an negative Example Line 1 Passive SIM Precursor 480 Produet 225 Lune 2 Negative SIM Precursor 612 Product 375 Line 3 Passive SIM Precursor 452 Product 225 Line Negative SIM Precursor 686 Product 315 Multiple Time Windows Several tme windows can be set wehin one scqustion Each tme wedow can alaws Full Scan SIM MSMS or mied peralons and ine Request Dwel ime can be set appropriate Scan Channel Validation Enable or sabie in MS Data Review as flows From MS Data Review cick Preferences and he select General 2 Open the Vaaton ab and select the Validate Scan Funcions checkbox areik to ear Enable t you change the tme tor the star at retention ime value aldaton wii snai ie retento Imes o at peska proportenaiy M Datis Changes you make wil nat be in sync ae ae Ses ee Scheduling Timed MRM Transitions Ov
65. or mere i a faut men ne LED s ed Oerwes the LED green Component Status Display The Component Status display is in the right prion ot he 450 60 Status and Conto window Selec 1e component iom th drap dom box ree rae Fadi nio nab pea Tons perpe Column Oven Status Camon sians acte Feo No Sere DC enir oes En co Description len oven i Renay Yes he sis mechan s andthe pigs 020 mine een fo iate pared AutoSampler Status Ts geten soriy to on docs at rave an B100 Anson or 8410 nem Description E aaae te o IU ak 17 meu 2t Sample Delivery Status Description Revver we coigued na OGD Sepa UT Desi Sate Erergued Sate Spe Pear pt Ed sme Serer Space Sere on et os et Bpass Bpass 1177 Injector Status fem Description R in tna 1177 etr component has oom Dci e 1177 ctr ovar comparant i turme ol or on a te Display 1e 1177 acr oven programmed temperature seting in Dio 1e actual oeae at the 1177 injector oven at te 450 Sr I Zer a site othe 177 macte method Ems H e O 1079 injector Status em Description Depa d utin re 1078 reor component has occured Begs e 1079 ctr ovan comparant i tumed ol or on a te
66. ow away om Pe 220MS or make tens Set iaa syne tmp aite mu a o dde mon Seo the 300 Soros LENS and GENS 400 Seres GC Quadrupole raro Supe singe pone 7 ick Advanced Opa and soci he Vaive and Syringe Punp ab 2 Secum Dire rca vate The system canton he Sitcom started lien 3 Ene Use Syringe Pump tpe ne flo rt anra te ceas bor LC MS Manual Injections Manual Injection Pune be switching valve as shown To do manual jection do he ftowag 1 Monitor o baseine using the Plot Te tool in the Toola menu gt Atter the baseline stabi zes lek the Traffic light and the Collect data fle inden opens TOE TR ed Tao UTE To re T HT SOR dai me NTE s uo RO emm sei Eu SIFT mm ans ee cac tne 3 Cick Fl naming methods io open e Automati File Naming window 4 Cic Auto inerement Type in he name ot ne data fie gt ne for Sequence v and 2 tor digts Ware i Browse o Teva cr elc an sang Wena Wa YOU May WE 1o use as rot forte Me name o your sample reper ma puc SI rm 7 Complete te Collect dta windon 3 Clck Apply and you re ready to collect data cick Stat NS 3 Check hat he valve lin e ba poston 10 Oven 5 using valve loop wih 3 o4 times the ole Push the on on the ave cover Alow at east 1 minute between recion orc ihe vave con n he Quad en ipea colecionby aparing Pe Colect ia window and ccr Siop GC MS or LC MS Injecting Single Samples To do
67. pen Sampetiss Yom he Fie menu Begin Sequence Begin an open Sequencels You can change methads and S mpi is dug an automated ru Open ha Sequence nom a Fle menu Begin At Start automaton at a parca sequence or SampleList ine and run rl heat ine is complelad A Sampiel st or SequanceLst must be open to iae tma feature Suspend Automaton Stop automaton atier he ast completed sample Resume Avomaton Star automaton er was suspended Atomaton starts Won where Iwas suspended Siop Automaton Stop automation mmedatoly and reset al modus Reset Modules Bring he vaeus modest thai ready states Enable Automated Printing Atow printer Aanctians to wok during automation Recalculate Menu The flowing shows he Automation menu and describes he optons Begin beria Sar an automated Recacuatoo A Recalculation st must be Opened under the Fle menu Begin at Selected Recalcis Line Process a Recakeulation Ist starting wih a houen ne and proceeding wih te res heist View Calibration Curves Vw curves ori paa compounds in the Solve memod The Celbraton Curve a saved a part c e Ve NOTE Recskuarons canis done m System Conta orm HS Data Fever From the command Quantaton select Process even Recs Lal Processing 2 Roca List takes more me whan dane rom System Conta an ba MS Dala Review but has he advantage of alowag AutoLnk lnclons such as aulomated tepari wih Custom MS Reports templates such as ToxProPus or EnvroPro Erro ans
68. ple ot avere ese sana wouid deposit on fe source and the source aye mor ret cano The delay parameter awichas e ret Whie the tows diverted tom he source you can delay data cotecon in the Hnuston Method The syinge pro can be programmed ta provide make up fw athe AP hte 1e LE efte verted Gems se he Detay parameter to turm the ament on aar a tme interval Use he in EMS rus o prevent the ament am being damaged or broken wile me solvent peak elutes Acquisition Method In Method Bulder open he Acauston Method Enable Use delay and enter ie oia me in mous MS MS Breakdown The eann Curve makes MS MS method development easy Te sample Purdiced inta the mace spectrometer API source wah a syringe pump A precursor on is selected ar MSMS trao cuni he precursor on can be edn e pierced node by etin SW n a Cui tex Fd wort xxl The dered mass To create a Breakdown Curve do he flowing 1 On the Toole menu cick MS MS Breakdown as InGOMS mode your oniy cholos Q2 colision ct I LCMS mode you can select Q2 Colin Cel Breakdown or API Capilary Breakdown 2 Olek Q2 Colston cl The Grete a breakdown curve window opens 1 Enter the precursor ion n Parent mas 2 CickTum on CID gas Wa about a minute tor itto stabilize 3 Solec elther Normal 25 V stege or Fast 2V step Cllecton speed hor S adequate or mast condana 4 To save the data to a method select Enable select
69. r you can teave out te un DETECTOR 1000 DETECTOR 1000 but eai 100 ok while ens 1 100 is ambiguous 10 When using pring statements on the cot ie or tha sting you ype Stats wah or or VERSION you dont have o type e era CR EI System Paw Macros When he Status Window s opened a Cit PML delat INIT and a Proc PM defaut QUAN INIT are botn nun 2 inthe Colect menu oobar star button a Ct PHL is defined whieh runs wen e tie being colecied s opened 3 Inte queue menu Colect Queue edt bution a Proc PML dened which uns uen the No being cotecodis cased 4 Aran sepan outside the aron can request a PML tough the Windows DDE mechanism Using the MSGBOX keyword you create and run PALS x Some Pi un when you ck on butions or menu ten re menu Reset INIT Fie menu Standby STANDBY Status conto menu CGON CGOFF CION CIOFF CIDON CIDOFF AutoTune menu ATUNE ATUNE2 ATUNES ATUNES ATUNES ATUNES peran manu DIAGNOSTIC test number Tune Tale menu ptmae bution OPTIMIZE Status probe butor PROBE MAIN Species Boling Pi Range menu compute bution SHELL ekg in Chro CHRO CLICKenrtype n ope sn nore substance kon pe CHRO sestas punon 1o see where ype type n and Other popular Pias are a PAR Dau Oks Pa Gus M SCAN meo Sw Help Files for PML Clck Hep in the System Conti window to open Some PML Procedures The Ciotti pmi macro closes the CI gas valve pints Evacuating CI
70. rements omerion atos re required ciek Edit ne Tune to target ratios direc wend gt M acting te ion ratio ist input tho o mass inthe lett column and the target ato relative o me base peak i th nghi column A base peak with 10076 rea must e in be iat CSc Save Mea necare rais nt mo m zm see mee ee x Inthe Tune to target aos direct window lek Proceed wth tune button 7 M the on ratos selected require ped reduction over 50 a marning window appears bere te program begie ring Do nol reduce he an peake ta drastisiy because much sey i et the peak reucdong rele han St elek OF Mer tuse 1900an re erdt ov rae su Merl ades eO remedy seater e Qnem Maris aie 100 sera etree MD ome WIDE E ATER sed mt arti gs cibos net uch th yay toast se Pd e B Atter the tune completes save the new tune tle The system does nor tomatic implement he new une To element fe new tune open the ane fl using mo Restore une tables in o nemen Status window a Standard Compound Editor GC MS EI Example 7 Cick Eat Standard Compounds the AutoTune widow and he allowing opens 2 Cikk New to detine a new standard compound 3 Type a name tar te new standard compound 4 Enable te Clear al elds bo to clear entries in th Standard Compound alog box 5 ook i d DEN IT T Ta come ECE edic HE 31 UEF reef LLL fms nd ieee ome 1 tome G T ime sein tme 27 ESO O OR S a Standard C
71. s Segments can be created foc Z You can do any o o povous sean modes or mised sean Podes in any tne segment and selec einer postive or negate play Scan Channel Validation Enable or ible MS Data Row as foie 1 From MS Data Review sc Preferences and then select General 2 Open he Validation tab and select the Validate Sean Functions check Enable you change the tme for th star at retention ime va aidaton wil enat te retention Imes o i peaks propartonal Disabled Changes you make wil at be in sync TIT eng ee amr MS MS Operations Produet lon Sean Scan fragman af a patcr oo The precursor ion is Selected in OI In G3 the start and end asses set e scan range trie Productions Example Precursor ion 219 Product scan 50 250 and alsin energy vs Precursor lon Scan The precursor ions ol a selected product ion are scanned Dita and end mass i setin Qt and OS i serta n desired produet n Example Precursor scan 100 500 Product ion 195 and a Collin enemy ot Neural Loss Scan Precursor lans are scanned in O1 O3 scans the same fange mins e neural se mass Example For oss o 2 18 water precursor scan 100 300 product scan 82 222 Sean copay product rs esl Vom D precursor ans ong a neural Selected Reaction Monitoring SRM Ts process monitors ono or more MSMS Vansitoni ot a precursor ian agnentng wh a speci energy no a produc lan Tre eagle sh sige rao stu ore cn e Example Precurs
72. stive or negative ion detecton The posee n party ot each suree e shown acms ra EI postive S Z and nepatne IS toms SR ESI postive and negate e Saa and negaive Capillary For LCMS instruments aniy Erter he absolta value ot e Capa vl or Saen ln In e scan metnad etd Bulder enters me corect agn Collision Energy For tile quadrupole struments oniy Determine the Caliaon Energy or each reaction and erter tin the Mas List 5 Seines coeion energies m elaton vots for speci ASS reacicns used i the coason cal Retro te 300 Senes LC MS and GC S 400 Sees GC arava Manual secton MS and MSMS ted Dwell Time ao the Requested dol tine ot each scan channel The shorer e duo ime he eae he number ar data points for he warstion Spec ether he San neo the dwet tme I nero areal lean ranges a a angle mod segment Mey must al can ae same rata despite De requested sl tne The sotwareQves sac mass range a dwel me according a he vera Sean Tane and me relative sizes o saen il sean ange Mass List Toolbar Enter values in the Mass List in a simlar manner as a spreadsheet The options ie Aac Ireen Delete or Clear Al Ines Cu Copy or Pase om a eel or a ia Omar optiona are ta down a com and M down or neromont 1e vales In enum FD and The Mass Lst can be copa oor om an Exel Spreadsheet Tu men J sen mgl ca cer en nee Ra Advanced Options Use Advanced Options to acess
73. t and Printer Setup are described nme MS Workstation Sotware Reference Marca Edit Menu Edit Module Info Clck Edit and select Edit Module Into Each module has a message log and a documentation sereen Use the Module ntomatco Eatorto record prtrmance maintenance hous used or other Use Ada it and Delete to record comments Inject Single Sample Menu Use inject Single Sampie t enter sae itormaton betore making an bjeclon The Module wow must be n scqustan mode betore malong an section aid rro lek Inject n the botom et comer to begin eter a manual or an autosampler fection Selecta Sample Type fom te ist Use AutoLinkto ener commands and parameters Enter a command to execute a program ate ha data Ho accion e completed For mere information about other elds see he Salat and RecleLit Fda Secon nthe MS Data Handing Users Gude par number 3954038 For esame you can actvate a custom MS Report t pint me sample reports To run a Custom MS Report in automaton 1 Type tne directory path and e name af the Custom MS Repor template or nmol C VanteWS EPAS2S su Note That Custom MS Repora empl shouid always pe siared in tre VasanWS dreciory 2 Cikk Browse to select be command executable tle 3 Cick OK entering a selection Automation Menu The folowing shows he Automation menu and describes the mien E Begin Samples Automation begins at ne 1 and ends at ie last ne O
74. tatistics The Sasi area plays summary itrmasoo about tho most recent IMRM Tha was import VAM eges e D Toa JU ae nei ans pe Kamn rete kat DUE fas evo matinee e Sere Injecting Samples Thi secton explains doing a inge manua inen and programming mile Pclons using he autosampler To make a single Weston use neci Single Sample To program rage injections use Sample Overview Samples are nea using System Control There are our steps in data sequsiion 1 Create or meaty an existing method gt Set up a Sample or enter sample ntoeaton or a single sample 3 Prepare and load the samples on he autosampler none Startup Shutdown of LC MS The startup and shutdown procedures and he nection methods ae the same ior bam me Elocrosray lonzaton ES and Atmospheric Pressure Chenscal lonzaton APC metoda Startup The instrument must be pumped down and running betore you begin 7 Cick Turm Spray On in me Quad View to tur on the gasses and hesters 2 Atter the Drying gas temperature reaches atleast 75 othe st valve start he LC pumps gt Wast a tew minutes tor the temperature to stablize and cick Analyzer Om tum on he Detector Shutdown Tum ott e LC pumps gt Alow the LC pump pressure o crop down ard stabilize so he fow into the MS stopa lek ma Spray leon om cn the gasses ard heaters me Analyzes ums on aubmatcaly wren you tum af ha sore 320 MS LC MS Syringe Pump and Valve Us hsp vae o dver t
75. the Compound Table Select Curve Fit Selec he desired curve tt tom me a COE TES For mast catbraton curves use elier Linear or Quadrat TOTE For moe Wiomatan aout Te ego T and Rlaremos Spoctum Tabs and or intarmaton about Guaritation and Dala Handing sae Ine MS Sofware Relaronce Manual pari number 391496300 EI Security Administration Overview The Securty Administration window contains ree categorles ot security Parameter These teatures we loos tor compar wih CFR par 11 Passwords policanon Locking Fie Revision gare Cek ne Security button on the Woretaton Toolbar to apen the Secury Passwords om Descipon Application Locki ing aren Descipon They mave 1e te Lack Aetna etal to widows sessing cie o paas aos Nee scons ced bythe Seay Adan sty ete tere Senn hasta tepimion diner ea Desciplon EIL LIEGE File Revision Settings Description ag ris we em re METER Re ani dy e dad tow pcs cave cts ae i pte ey atic deer GC ese CONS EI Description Configuring HPLC Modules Overview Configuring GC Modules Overview Sica 40 Sarias GC commuricates van me ora over a sanat Eihenat connection your system can sasiy be expanded Tom Sates GGange Woran coriguat
76. tor ply area 1e agora 10 Carousel ply Il shows a graphical epresentaon of e 8400 ar 3410 Carousel Tha dia orapnicaly snows Wh val are t bo sample whch vals have been sampled Which als ment bem sampled 1 Below these spay areas i the Chromatogram ana A selection box above he civomatoram alus you to Sle e channel tr wich yau want to vew The reat irre chromatogram 450 GC Operation Display The 450 40 Operation display is in the left poten ofthe 450 GC Stats and ania wed VEN Ten re 3 Description E easy PE Star PRE T ris Moto Surgite Comi rH Ra ead Rang tbo te gh re ext necionn ha Sequence Equivalent w presara Stop on Temperature Zones Status Display ma EET m srt E FOLE S mo sn The temperature zones are played nthe middle af he 450 00 Statue and Canto window The daplay cds he sepas and actual temperatures al The coburn oven tn tee zones tal can b cooled and esta and De tes zones that can only be neated The display 1e Fault a faut occurs and OI The zone oven of the zove ras not een contgured by the 480 GC hen MA s displayed De setpoints and actual emperature Cicing one a e Temperature zone annis displaya the conespondng component Sas a ie Component Sas Display The LEDs indicate the status o each temperature zone the zone nat onlgured or me zone ovens of Ie LED i ray ho temperature zone s Not Rendy
77. tup dalog displays the hardware configuration inornation input rough e GC kayeaad and crenty saved nthe GC memory Use P Pint buon 1o printa copy for your records The OK bution ses he dialog Use e ter buone t input additonal contiraton rormalon 8400 Dual Mode Setup The sno AutSanplercan inject into two contiguous injectors in the same chromatographie run se The 8 00 Dual Mode Setup t detn the contguraton ea wnen hat capit e voked The 8400 can inject irio ont and mde ride and teat jac and each ot me iyecors can be connecied tough eat cour fo any he insaled detectors When the 8400 AutoSamoer or B410 Autoinjector i used in Dual Mode use his dag box to assign he decor channels 1o tre data Te for he concsponding Sample in Dua Mode operation o dra hos are created one for ho it Sample injected ha oiner foc n second sample injected The samples atro neci are dried by thee 82008410 norton Poston s ether Poston or Poston 2 The user spoctes which detector iomann o associate wth each SOn Postion 8400 8410 SampleList Window Extensions When a 450 GC cons an 8400 AuoSamoler or 8410 Autoinjecior me Sarai tor Inat nsrament conan tre lowing deve dependent fis Many or those eLa elds are ony shown and avaiable lor eng when theo AutSompler or B410 Auloinecir s used in Dupicate Mode or Dual Mode See e help on me gena SompleL s Window fora descepton ot he ek inat ar
78. umber Tha available row and column numbers depend onthe ee plate configure 48 standard vials A tough FB meen At trough me a84 wo late A1 though P24 Use e Waal ao random acess any wel ria For example you can parodia recae tom aang standard a Mad cao For the ProStar 420 te Vi ld spectes e sample val as a combinaton f Tro ype te and Val uter The avaata Tray pee and Vai numos aeperdonine Tray Sager contgurea Yau can ray accesa any val Inte Wast aos eid erter a wash volume in e range of 300 a 9999 p or enter O tor no wash is Inte ne Rotes d dene a pec song program 1a be dre rte De sample s ecd When dong an Alomar you an program the Auto Sampler w aspirate aud fom a wel a ora egent al and Yareler Toe same wel val Fo wh an eco ir be ade Yeu can ma te sangle by sprang and dispersing secte amount You can program me AuoSamoierio wal lora spei me naval Use the Atomi Steps dalog to select Action Poston Aunt Speed a t syringe sd Hah ofthe needle The flowing an example ol an Autom roue E ot ee eee rare ot 240 steps can be programmed in one Autom routine Aner tenn she step erter a name for Autor Program im te box ne o Cancel and ciek OK Tho program i saved in ne Sarge and can be sported 1o ater SampleL ats Anernatveiy Autre Programma om ater Sint it can be pared i ne acia Samoet iet using impart The User Progam clura is enabled I selected in tne Ijecton Mode feid for the samp
79. umber 395405800 provides post aequo daa handing procedures ard aske Tove procedures include aertivng lema randa sig nan grater Gg te pes nd peram 450 GC User Manual This manual par number GET Is included on ho MS Workstation CD and describes in dtl me operation ol ha 450 QC 430 GC and 431 GC User Manual This manual pat number CP501406 i included on th Varian MS Workstation CD ana describes deal he operation f e 91 OC DIP DEP Solids Probe Manual Th manual part number 395414900 which comes wih the optional DIPDEP probe tor 1e Soo MS orna 320 MS GIS descibas he operat ot the BI DEP sods probe Workstation Toolbar Overview H i Y H e SEE YS SESS ot Sample Recall Fun serie ihn using Sane Quick Link Buttons The Quicklink butone aro on te right f he application cons They corespend domos recon used Data Fas and Metodo Dru Most Recently Used Data Fe Display opns tor he Most Recent Used Data Fie or select a dre data fie Pr mne kt most recon used tes Lo Most Recently Used Method Display operations ha can bo pertormed on he Most Hacen Used Method or soci a iren metod le tam me Iso most recently sod method ae E c mea Me Workstation Toolbar Options Moving the Workstation Toolbar Move the Workstation Toolbar ta any edos of he Windows sereen Cick an area fme Worstaton Toobar
80. usts e deiactor vollage ove the dynam ange EDA s calbated dug P olune see AutoTune Optons on page 34 EDR Maximum Use he otium ted voltage or maximum gan tom he lest EDA calvin sel T MSS experiments Fixed Voltage Enter ted voltage or he entre method or erent nes foreach segment using Advanced Optone The detector can be programmed to turn otf when the method ends Ces va ires Scan Width Set ne maximum peak scan window for an in in single ion monitoring The etsi vue 070 neeaae ta eue 10 change he resoiuton on the Scanning quad Adjust t according tothe resolution For example he fesolaion la 5 0 on scanning quad pole SIM wath sel the SIM Wat between 107 and forth largest acie Sonal m Timo Segment Arrows Uee the arrows to select tme segments Use Add seg o add a time segment and Remove seg to remove a tme Segment om e scan manod Stara Retention Time defines he retention te start for each segment HH pue up Scan Channel Validation Validate Sean Function Indicates 1 Scan Channel Validation is enabied cero Aiso See MS Dala Revie General Preferences Scan Channel Valitation ensures that the scan channe speeeatans tor all compounds are corecater vareltns re sed or deleted in me sequisiton meo t you change segment acaso tines scan channel valdan totaly updates the compound reanton ines you select a compound that spectes a deed harap a maming massage pere The masing Yanstion
81. wing Select the older and hen he tle name uan Bamin demam me Co ae eats E sl Cek Recent Files to display them and select one Build a New Method Clek Create a New Method File and cick OK to open e Method Bulder Wear IR eeu eer s 2 Select Instrument 1 The confgured instrument is added to he method 3 Oiek Nent Ci Select a detector Atter te data is acque you can add data processing information othe method 5 Oiek Next rm ET e cee 5 Cleat sections tor postun processing Ada MS Data Handing t erter egratoo peak names and ther parametere Add Standard MS Reports to Use e standard reparis 7 Oiek Next L me um ce x e the imation on ihe Cont coniun sen x Clk Next 10 Review the contguraton Cick Back to make changes Cick Finish to conti 11 The new method is displayed win a navigation ree on he et side and the method workspace on eint Gick a ee enty a open mat method Spon Add a Varian Instrument to a Method 1 Select Create a New Method File and clk OX 30 Select an avalable module Yom the lst and ick Add to male it one af te Gortied Modus Othe options are Add Add AI or Remove Ck Next cum DET e S Continue win stap 3 of Bull a New Mend on page t 450 GC Methods 450 GC Windows erae in the Method Bulder to display the

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