Electronic pipette
Contents
1. es ronlabs smartpipette Operating Manual single channel DETAIL VIEW Support 2 4006 296833 Contactus Aboutus_ smartpipette Electronic pipette Silver nano series Evolutional pipette design superior user friendly product support Innovation In Action Ronlabs service provider to global premier RONLABS SCIENTIFIC INSTRUMENT CO LTD Content 1 Introduction 3 2 Packaging contents 3 3 Product description 0 4 3 1 Screen instruction 4 3 2 Button functions 4 33 Menu 5 4 Operation 6 44 Battery 6 4 2 Tiptitting and ejection 000 7 43 Startup 8 4 8 1 Start and stand by s S 8 43 2 System set up 8 4 3 3 Select pipette mode 9 43 4 Parameter set up 9 44 Nine pipette modes 10 444 PIPETTE mode 10 442 RPIPETmode 13 4 4 3 STEPPERmode gt 14 444 MIXmode 15 445 MANUALmode 16 44 6 Four special pipette modes 17 A PIPETTEAMX 17 ee STEPPER SEQ i sts S 18 nn ASPIRATE 24 Lu GEL 2 45 PROGRAMLIST 23 Programpre st 23 Parametersetup 25 5 CALIBRATE 26 Calibration environment and facilities 26 Calibration procedures 27 Configuration for calibration 28 6 Troubleshooting 31 6 1 Solution for trouble shooting 31 62 Leaktest 82 7 Disassemble and reassemble 33 74 Disassemble 33 7 2 Reassemble 7 8 Maintenance 41 8 1 Cleaning and decontamination 41 8 2 Easy maintenance 42 8 3 Replace batter2. S300 C1 C2 C3 C4 C5 611103 612103 613103 614103 615103 616103 47 1200 D1 D3 D4 D5 A 611104 612104 613104 614104 615104 616104 611105 612105 613105 614105 615105 617101 618101 611106 612106 613106 614106 615106 617102 618102 F4 F5 F6 Tips order information 682021 R 200 R 1 200 L 96 Rack 5Rack Bag 10Bag Case S100 682030 R 300 1 300 L 1000 Bag 20Bag Case S1000 S300 682031 R 300 R 1 300 L 96 Rack 5Rack Bag 10Bag Case S1000 S300 682120 R 1250 100 1250 L Clear 1000 Bagj 5Bag Case 1200 682121 R 1250 R 100 1250 L Clear 96 Rack 5Rack Bag 10Bag Case S1200 682500 100 Bagl 10Bag Case 682501 Clear 54 Rack 10Rack Case SomL 682600 1 10mL 100 Bag 10Bag Case S10mL 682601 R 10m R 1 10mL Clear 24 Rack 10Rack Case 10mL 682605 R 10m E 1 10mL 100 Bag 10Bag Case S10mL Above are recommended partial model please refer to www ronlabs com for more information 48 Accessories order information Battery Rated capacity 900mAh Maximum charging 4 2V Voltage Note When use with Ronlabs original product Battery charger ter 100 240V 50 60Hz Max 0 15A Output USB 5V 500mA USB cable 693301 RA MT1 Input AC Lubricate Above are recommended partial model please refer to www ronlabs com for more information Any modification of this user manual wi
3. 2 When mixing process is completed pipette stop Extra liquid is still in the tip Press PIP to purge 4 If required replace the tip before next operation ou Notice Press for more then 1 second will abort the operation instantly 4 4 5 MANUAL mode This mode allows operator to control D ul volume start and stop the aspirate and HANUAL dispense 1 MENU SPEED Parameter set up VOL LIMITO Value between valid range is available for set up Factory default setting is the maximum volume given by specification IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of MANUAL mode Select MANUAL mode set speed operation is ready 1 Immerse the tip perpendicular to liquid and press and hold until desired volume of liquid is aspirated in to the tip 2 When the screen display the volume has reached the target 16 release the button Do not release the button until the volume has reached the target 3 When aspirate is completed Remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid 4 Press button to switch from aspirate to dispense The screen will indicate the direction mark change from to Press down PIP until dispense is completed If maximum volume of the pi
4. A 9 MENU SPEED Notice If CHECK PISTON is displayed the message indicates piston is moving irregularly Disassemble bottom section to find out the cause of the problem Please refer to chapter 6 for trouble shooting and remedy Stand by If operation is paused for more then 2 minutes pipette enters stand by mode Press will resume to previous state Note 1 If liquid is not dispensed during pipetting process or the pipetting cycle is not completed pipette will not enter stand by mode 2 If pipette will not be used for long period of time remove battery block to ensure battery life 4 3 2 System set up Press key to enter main menu Press key to select setting Press OK to confirm The parameter setting of pipette mode can be done from sub menu a CHANGE MODE n SET PARAHETER COUNTER OH 00 ul PROGRAH LIST BUZZER ON BRIGHTNESS 1 PIPETTE CALIBRATE BACKGROUND 2 AQ MENU SPEED MAX VOL Max Volume f Only available on S1200 model which can be set to 1000 L or 1200 L other models such as 10 5100 S300 S5mL S10mL are fixed value for this parameter COUNTER ON or OFF to select whether counting the cycle is required BUZZER ON or OFF to select whether alarm for low battery is required BRIGHTNESSJ 1 6 level can be set according to personal favor BACKGROUND Customize background color to be black or white black 1 white 2 Set upf Press selection key to select setting item then edi
5. OK YES 1 BOO ul 0 OSU YESH EDIT PIPETTE C1 9 MENU SPEED Selection Up or Down Change Parameter Select Parameter Right Speed Adjustment EXIT Start or PIPO p Ep Start Aspirate or dispense Tip ejection button Note If Picture or operation instruction described in this manual is different from the product Please always refer to the product Reset Restart and return to mail screen and factory setting Start calibration mode USB Power supply Battery Holder Install or replace battery Finger rest Suitable for either right or left hand operator 3 3 Menuf Main menu Submenu 3rd level Set up level CHANGE MODE PIPETTE RPIPET STEPPER MIX MANUAL SPECIAL PIPETTE MIX STEPPER SEQ ASPIRATE GEL SET PARAMETER Menu PROGRAM LIST Pir CHANGE MODE SET PARAMETER SAVE BO aici SETTING MAX VOL available on S1200 only COUNTER BUZZER BRIGHTNESS BACKGROUND CALIBRATE Noter CHANGE MODE serve the same function as CHANGE MODE 5 4 Operations 4 1 Battery Note We strongly recommend you fully charge the battery before using the pipette You must only use RONLABS original battery and battery charger Any damage caused by using substitute accessory will not be covered by warrantee Please charge your battery indoor Do not place the battery in contact with metal surface Battery disposal should be done in accordance to local regulation Sep
6. PIPETTE VOL 5mL MIX VOL 4 5mL MIX TIMES 5 IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of PIPETTE MIX mode select PIPETTE MIX mode set all parameters operation is ready 1 Immerse the tip perpendicular to liquid and press PIP liquid is aspirated in to the tip 2 When aspirate is completed remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid 3 Press PIP to dispense the sample 4 Press again to perform MIX function MIX function stop when the cycle complete 5 Press PIP again to clear the rest of liquid in the tip 6 If required replace the tip for next operation Noticef During mixing process press for more then 1 second will abort the operation instantly STEPPER SEQ This mode fill in total required volume Total required volume Vo1 Vo2 Vos Of dispensel 3 FT aL and dispense a number of different volume FALL samples Extra volume of sample will be 2TEPTSE aspirated to achieve the best result AQ HEHU SPEED 18 Parameter set up VOLUME Value between valid ranges is available for set up Vol Vo0 Vo3 The volume to be dispensed each time Sum of the total volume should not excess the maximum volume of the pipette If volume of the last dispense is s
7. number of times left to dispense is displayed on screen 4 When total number of times to dispense is completed there are extra liquid in the tip Press PIP to purge the extra liquid 5 If required replace new tip for next operation 4 4 4 MIX mode This mode is achieved by repeatedly aspirating and dispensing according to the parameters Diagram on the right indicates 200 L of liquid is aspirated and dispensed HENU SPEED for 5 times Parameter set up MIX VOLE Value between valid range is available for set up MIX TIMES Times of mixing aspirating and dispensing cycle 1 time maximum setting value is 200 Counter will not display 3 digits 100 200 This parameter setting can only be done from SET PAREMETER menu Different model of smartpipette has different factory default setting as follow S100 MIX VOL 2 Lf MIX TIMES 5 S1000 MIX VOL 20 Lf MIX TIMES 5 S300 MIX VOL 20 L MIX TIMES 5 1200 MIX VOL 200 Lf MIX TIMES 5 S5mL MIX VOL 1000 LO MIX TIMES 5 S10mLO MIX VOL 2mL MIX TIMES 5 15 IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of MIX mode Select MIX mode set volume speed and times operation is ready 1 Immerse the tip perpendicular to liquid and press PIP liquid is aspirated in to the tip
8. 00 600 2 00 1200 4 50 500 4 00 S5ml 500 5000 2500 8 00 5000 5 00 1ml 20 0 10ml 1000 10000 5ml 21 0 10ml 25 0 Note error lt 0 02 lt 0 02 lt 0 04 lt 0 04 lt 0 25 lt 0 20 lt 0 15 lt 0 25 lt 0 40 lt 0 30 lt 0 50 lt 0 50 lt 0 50 lt 1 50 lt 2 00 lt 3 00 lt 2 50 lt 5 50 lt 6 00 lt 5 0 lt 10 0 lt 20 0 ISO 8655 Random Systematic error 0 12 0 12 0 12 0 12 0 80 0 80 0 80 0 80 4 00 4 00 4 00 4 00 16 0 16 0 16 0 16 0 40 0 40 0 40 0 60 0 60 0 60 0 Random error lt 0 08 lt 0 08 lt 0 08 lt 0 08 lt 0 30 lt 0 30 lt 0 30 lt 0 30 lt 1 50 lt 1 50 lt 1 50 lt 1 50 lt 6 0 lt 6 0 lt 6 0 lt 6 0 lt 15 0 lt 15 0 lt 15 0 lt 30 0 lt 30 0 lt 30 0 If there is no special remark the unit of above data is uL Test environment PIPETTE mode speed 9 45 Appendix 1 Correction factors for distiller water as a function of test temperature and air pressure Z value in microlitres per milligram Co la Js Jo o a0 Jams 105 Appendix 2 Parts E L 82 Parts SO Grip section white 1 Tip ejector button S2 Piston holder A1 F1 Piston A2 F2 Connecting nuts A3 F3 O ring A4 F4 Tip holder A5 D5 Tip ejector E5 F50 Upper tip ejector E6 F6 Lower tip ejector 611101 612101 613101 614101 615101 616101 100 611102 612102 613102 614102 615102 616102
9. 7 ISO 8655 Standard Maximum permissible error wea ou 1 10 L 5 100 L 50 100 20 300 L 150 300 er error Random error 1000 1000 10000 L 5000 E Configuration for calibration Smartpipette has complied with ISO 8655 industrial standard for permissible error If different standard should be applied proceed to configuration with following steps 1 Press and button simultaneously then press reset button Electronic pipette will restart and prompt PRESS PIP Release and button Press to enter configuration mode Screen display CL in red indicating calibration mode is ready JJ fa J Q D NO oO 100 100 1200 L 1200 500 500 5000 L 2500 5000 40 40 60 60 60 mi CL 120 ul PIPETTE 9 HEHU SPEED 2 MENU gt CALIBRATION OK to enter calibration screen CHANGE HODE SET PARAMETER CALIBRATE PROGRAM LIST V1 SETTING Um Uh 3 to select target value EDIT blinking press key to change value the value should be the same as average volume obtained from calibration measurement procedures OK to save CALIBRATE V1 128 3 ul Um 689 84 ul Vh 1200 60 uL 4 Repeat step 3 until all value is verified OK to save OK again to confirm Screen will return to previous menu 5 Press button pipette will restart Press to enter main screen The system has returned to normal state 6 To confirm if verified value has been saved MENU key to select CALIB
10. RATE EDIT to confirm the value If value is not correct repeat the process Note Only the calibration value verified in calibration mode with CL character on screen will be saved into system memory In another word the calibration value verified in regular system no CL character on screen will not be saved into system memory Calculation of mass loss Conversion of the corrected mass to volume V w e x Z V Volumel L w Weight mg e Evaporation loss mg Z mg L correction factors Evaporation loss has great influence to small volume pipetting Therefore the calculation of evaporation loss is essential to 29 accurate operation Methods Fill certain volume of distiller water into a vessel that is weighted Observe 30 seconds and read the volume loss and calculate the volume loss per second if 3mg is the volume loss for 30 seconds volume loss per second is 3mg 30s 0 1mg s During operation the correction of mass loss can be done with above methods operation time x volume loss sec However if an evaporation prevention device is used the consideration of evaporation loss can be omitted Notice Density and volume of water under different temperature and pressure vary Correction factors for distiller water as a function of test temperature and air pressure need to be taken into consideration Refer to appendix 1 for more detail Systematic error Systematic error is to calculate the difference between the
11. VE ENTER 5 Select CHANGE MODE OK to set up desired pipette mode such as STEPPER or others as follow PIPETTE RPIPET u HIX e MANUAL m SPECIAL OK 23 6 OK and return to step 3 Select Save OK to store the setting as follow this program now has been saved as system default or EXIT to return to previous menu CHANGE MODE SET PARAMETER ENTER Parameter set up Parameter set up for quick access program can only be done from program list Detail steps are described as follow Take STEPPER mode as example If the desired program for STEPPER mode is to dispense 100UL for 10 times 1 MENU scroll to select PROGRAM LIST OK scroll to select Pl EDIT scroll to select CHANGE MODE scroll to select STEPPER OK P1 now is set up to be STEPPER mode 2 Scroll to select SET PARAMETER OK to enter set up screen Scroll to select sample volume value in red indicates the parameter like to be edit EDIT blinking edit ready selection key to change the value to 100 L gt OK to confirm and return to screen as follow ana tO ul x US SET VOLUME Pi 9 EDIT EXIT 2 Scroll to select times of dispense EDIT blinking edit ready key to change value to 10 OK to confirm and return to screen as follow 24 Scroll key to select aspirate speed in speed gt EDIT blinking edit ready scroll key to change value desired speed OK to confirm and continue the se
12. actual volume and the piston operated volume A V V A systematic error y average volume Vo piston operated volume A 100 xA Vo Random error Random error refers to the difference of average volume of measurements and volume of single volume of measurement The result indicates unpredictable factors also called standard deviation S or coefficient of variation CV S standard deviation V average volume n measure time Vi volume of single measurement CV 100 xS Vv Note Due to the facts that calibration requires highly facilitated environment and special experience and knowledge we do no recommend on site calibration by customers If the situation require send the device back for professional service 30 Before you deliver the devices make sure it s free of contamination 6 Troubleshooting 6 1Possible problems and recommended solution Message with CHECK PISTON Lack of accuracy or leak Retention In tip piston position is inappropriate lubricate for piston is insufficient lubricate is solidified particle on piston surface particle between piston o ring and cylinder tip fitting is inappropriate the tip is not compatible to the device particle in between tip and tip holder tip is damaged speed for aspirate is over high tip is removed from liquid sample too soon piston surface has dust been damaged particle between piston O ring and cylinder insuff
13. arate such battery with your daily garbage is highly recommended Never expose battery on extremely high temperature which may cause explosion Battery specification Lithium lon Battery Voltage 3 7V Capacity 900mAh Maximum charging voltage 4 2V Charging times 2 hours will achieve a full charge with Original charger item number 693201 Battery charger Specification Input AC 100 240V 50 60Hz Max 0 15A Output USB 5V 500mA Installing Replacing batteries 1 Press the cover of battery holder down and push toward the direction indicated on battery holder 2 Insert the battery block accordingly 3 Restore the cover of battery holder Battery state indication on screen Green Full charged Black white background or White black background Partial charged Red Low battery indication recharge or replace battery is recommended Insufficient battery indication When power is extremely low and insufficient to support mechanism during operation process main screen appears PIP indication Note Following icon and alarm will remind you to recharge or replace battery when PIP start button is clicked again When you see the reminding message recharge or replace battery immediately The power will go off in 90 seconds Recharging battery Before connecting charger with power supply make sure the input voltage is compatible After Connecting charger and pipette with USB cable insert plug into elect
14. e lower section Rotate lower part of the tip holder counterclockwise to unscrew it and pull down from the upper part Cc ne 1 Bottom 4 Remove o ring D3 Hold on D4 orA4 B40 CAU rotate connecting nut D2 orA2 B2 C2 counterclockwise to separate D2 and D4 remove o ring D3 or A3 B3f C3 34 1 2 5 Remove piston D1 Hold on upper part of pipette Another hand will bend the piston down as you want to break it do not pull it out The piston will separate from piston holder D1 or Ai B10 Ci Piston holder Remove piston with D1 Disassemble procedure for S10 S100 S300 S1200 is completed Disassemble procedure for S5mLf S10mL 1 Battery block removal refer to the same process of S10f 100 S300 S1200 2 Remove lower section of tip ejector E6 Lie the pipette horizontal screen face up both hands thumb and index 35 finger hold on the intersection of ES or F5 and E6 or F6 apply pressure to separate the two sections Index 1 2 gt Thumb 3 Remove upper section of tip ejector E5 Keep the tip eject button depressed rotate upper section of tip ejector E5 or F5 toward clockwise until the hinge pop out A J E2 2 ES E4 I 4 Remove lower section of tip holder Rotate lower part of the tip holder E4 counterclockwise to unscrew it and gently pull out until piston holder presents Bend the lower section 36 in gray the
15. erent liquid sample Chemistry How Cleaning and maintenance Aqueous Disassemble bottom section of load the pipette rinse contaminated Buffer parts with distiller water leave it to dry naturally and refresh lubricate on piston Disassemble bottom section of the pipette rinse contaminated Inorganic Use filter parts with distiller water leave it acid or alkali tips to dry naturally and refresh lubricate on piston solution Disassemble bottom section immerse into disinfectant for 30 minutes perform autoclaving with stiller water Use filter Infectious tips liquid Pipette mode Disassemble bottom section Cell culture Usefilter immerse into disinfectant for 30 fluid tips minutes perform autoclaving with stiller water Disassemble bottom section of Increase the pipette rinse contaminated Organic operation Parts with distiller water leave it solution speed to dry naturally and refresh lubricate on piston 42 Use filter Disassemble bottom section of tip the pipette rinse contaminated Use parts with distiller water leave it Pipette to dry naturally and refresh mode lubricate on piston Radiation solution Nucleic acid boil 10 minutes with amino acetic acid or hydrochloric acid buffer solution PH2 0 rinse with distiller water Use filter and leave it to dry by Nucleic acid tip evaporation or under 60 C or protein Use environment refresh lubricate on solution Pipette p
16. event sample overflow The function is appropriate for handling viscous sample Parameter set up VOLUMES Value between valid range is available for set up IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Fix Note 10 S1000 S300 OUT SPEED default is 1 1200 S5mLf S10mL OUT SPEED default Is 2 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of GEL mode Select GEL mode seiting up all parameters operation is ready 1 Immerse the tip perpendicular to liquid and press PIP liquid IS aspirated in to the tip 2 Remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid 3 Press PIP to dispense the sample 4 After dispense screen display PIP Press to clear up the rest of sample 5 If required replace new tip for next operation 22 4 5 PROGRAM LIST There are 6 quick access addresses for operators to store personal programs Program pre set Set up pipette mode 1 MENU scroll to select PROGRAM LIST CHANGE MODE SET PARAMETER m SETTING CALIBRATE 2 OK to access set up programs There are 6 addresses available Default is 1200 PIPET Scroll to select target address for customization 1208 PIPET 1208 PIPET 1208 PIPET 1208 PIPET 1208 PIPET EXIT 4 OK io enter set up screen as follow SET PARAMETER m SA
17. icient lubricate on O ring or piston o ring is damaged inappropriate operation retention of the tip is too high viscosity of the liquid is high 31 4 reinstall piston 4 refresh lubricate 4 refresh lubricate 4 clean or replace piston 4 clean up piston o ring and cylinder refit the tip replace with compatible tip clear up tip holder replace with new tip reset the speed refer to proper operation technique replace or clean up piston 4 clean up piston o ring and cylinder 4 refresh with proper amount of lubricate 4 replace o ring 4 refer to proper operation technique 4 fitting with low retention tip 4 use RPIPET mode rinse the tip few times before pipetting reduce RE Can not battery life is over 4 replace new battery Noisy insufficient lubricate 4 refresh lubricate during operation inappropriate 4 Calibrate with proper Lack of calibration require liquid sample precision recalibrate on high on viscose liquid special when liquid sample is 4 pre rinse the tip and liquid volatile or significantly calibrate with the liquid sample different from water in again density Tip fall quality of the tip 4 use recommended tip down or damaged tip 4 replace new tip hard to fit If you still have question or problem consult your local dealer for customer support You can also call 4006 296 833 for technical support in the nation 6 2 Leak test Leak test should be performed fro
18. ispense Voz The rest of 150 L will be displayed as follow Press to dispense Vos Press again to clean up the extra liquid ET 130 130 v Os v OU 5 TEP SEQ 5 TEP SEQ C1 39 C1 F9 HEHU SPEED PIP Operation of STEPPER SEQ mode Select STEPPER SEQ mode set all parameters operation is ready 1 Immerse the tip perpendicular to liquid and press PIP total required volume is aspirated into the tip 2 When aspirate is completed remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid 3 Press key to set up Voi volume of first dispense 4 Press to dispense sample of V screen display volume of second dispense Vop 5 Repeat process 3 and 4 until all liquid in the tip is dispensed 6 After all liquid is dispensed screen display PIP press to clean up the rest of liquid 20 ASPIRATE EE This mode works as reverse function of ARTE aia STEPPER It fills in equal volume in number of x LE ASP IRATE times and dispenses the total volume at once a3 total volume for dispense volume x times MENU SPEED of aspirate Parameter set up VOLUME Value between valid ranges is available for set up TIMES Times in this mode indicate times of dispense This parameter can be set up from SET PARAMETER menu not from main screen Note Times of aspirate x volume times has to be no larger then maximum volume of given specification The program will auto
19. iston mode Protein disassemble bottom section and clean with soap solution and distiller water leave it to dry by evaporation or under 60 C environment 8 3 Replace battery When the operation is significantly slow or acting abnormally due to low battery power or battery life recharge with USB cable or replace battery block as following procedures 1 Push the cover of battery holder and slice toward the direction indicated on battery holder 2 Replace with new battery block accordingly 3 Restore the cover of battery holder 4 Turn on the device and confirm the power state Note After replacing battery main screen and setting return to default setting 8 4 Storage Daily storage After operation hang the pipette on a pipette stand If the tip has any liquid never place the pipette horizontally liquid flow into handle of the pipette may corrode piston and cause damage If the devices will only be used once a while it is recommended to remove battery block clean and decontaminate Store the pipette in dry clean stable environment to preserve its life 43 9 Precautions and limitations Read this manual carefully and use the product according to the instructions Use the product within the limitation of chemical corrosion Do not aspirate highly flammable sample If temperature of liquid tip and pipette is significantly different the result can be very inaccurate Do not operate thi
20. le for 9 repeating operation of same volume The MENU SPEED diagram on the right indicates total of 1000 L will be filled in at once and dispense in 5 times x 200 L time Parameter set up VOLUME Value between valid ranges is available for set up TIMES Times in this mode indicate times of dispense This parameter can be set up from SET PARAMETER menu not from main screen Notice Times of dispense x volume times has to be no larger then maximum volume given in the spec The program will automatically reduce times of dispense if above calculation is violated Different model of smartpipette has different factory default settings as follow S100 VOLUME 2 L0 TIMES 5 S1000 VOLUME 20 LO TIMES 5 S300 VOLUME 20 Lf TIMES 5 1200 VOLUME 200 L TIMES 5 S5mLf VOLUME 1000 LO TIMES 5 10mL VOLUME 2mL TIMES 5 IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 14 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of STEPPER mode Select STEPPER mode set volume and times operation is ready 1 Immerse the tip perpendicular to liquid and press PIP liquid IS aspirated in to the tip 2 When aspirate is completed remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid 3 Press for number of times to sequentially dispense The
21. ll not be noticed in advance Please check www ronlabs com for newest version GS anne http weibo com smartpipette weibo com mead F ES http t qq com smpipette Scan this code with Bene your smartphone ronlabs BE QE Sipe oO mo SA CHENCHONG INDUSTRIAL DEVELOPMENT AREA LONGJIANG FOSHAN GUANGDONG PROVINCE CHINA Service ronlabs com www ronlabs com ronlabs Ronlabs All Rights Reserved SCIENTIFIC INSTRUMENT
22. lower section including piston is detached the same methods apply to S100 S100 S300 S1200 E5 i Note To detach the loser section you need to bend it not to pull out 5 Remove piston and o ring Remove E5 rotate counterclockwise to remove E4 or F4 from E2 or F2 remove piston Elf or F1 and o ring E3 orF3 as diagrams showed A A C A ce TTE j B E2 gt gt y E s Me D l xiy 2 I 3 4 So far disassemble of SsmL S10mL is completed Notice The grip section section that higher then piston holder should never be disassembled Any damage or malfunction cause by disassemble of grip section will not be covered by RONLABS warranty policy 7 2 Reassemble Reassemble procedures for S10 S1000 S3000 S 1200 1 Reassemble piston hold on bottom of piston D1 or A1 B1f 37 C1 gently insert the tip of piston into the piston holder as follow D1 2 Restore D2 or A2f B2f C2 by rotating counterclockwise restore o ring D3 or A3 B30 C3 as illustrated below connect tio holder D4 with connecting nut D2 by rotating counterclockwise 100 1200 pl ES 2 D2 A 1 D4 3 Restore ejector D5 or A5 B5 C5 Keep the ejector button S1 depressed insert the ejector and fit the hinge section the reverse way of assemble procedures refer to following gt 38 diagrams D2 P D4 D5 i 1 2 4 Restore battery block into battery housing restore housing co
23. m time to time to ensure the quality and precision of operation especially after maintenance or decontamination procedure Methods 1 Pre rinse the tip 2 Set the pipette to maximum volume 3 Aspirate the volume of distiller water 4 Maintain the pipette in the vertical position and wait for 30 seconds If water droplet appears at the tip end leak is confirmed 6 If there is no droplet re immerse the tip into the surface of liquid 7 If the water level inside the tip goes down leak is confirmed Refer to trouble shooting for solution on 32 Notice Volatile liquid or most of organic solvent can cause leak due to the issue of saturated vapor pressure Try to operate few cycle of aspirate dispense and test again 7 Disassemble and reassemble 7 1 Disassemble smartpipette is designed to be easy maintenance Disassemble can be easily performed by operator Disassemble procedure for S10f S1000 S3000 1200 l 2 Remove battery block Position your thumb and apply pressure to the cover of battery housing slice the cover toward the direction as indication Battery block is now easily to be sliced out x 1 2 Keep the tip ejector button depressed and grip the top of the tip ejector D5 or A5 B50 C5 with the other hand Rotate the tip ejector counter clock until you hear the click pull the ejector D5 or A5 B50 C5 away from the handle of the pipette 33 D5 D5 1 2 3 3 Remov
24. maller then lower volume limit of the pipette this dispense will be treated as waste purged out with any extra liquid Different model of smartpipette has different factory default setting as follow 10 TOTAL VOL 10 Lf Voi1 5 L Vo2 5 L 100 TOTAL VOL 100 LO Vos 50 L Vo2 50 L S300 TOTAL VOL 100 LO Vo1 50 L Vo2 50 L 1200 TOTAL VOL 1000 LT Vo 500 L Vo2 500 L S5mL TOTAL VOL 1000 L Vo 500 L Vo2 500 L S10mLf TOTAL VOL 10mL Vo 5ML Vos 5mL IN SPEED Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Volume of sequence dispense can be set after aspirate of total required volume If total volume 1000 L is to dispense in 3 sequences 450 L 400 L 150 L the setting example would be as follow 1 MENU CHANGE MODE select SPECIAL OK select STEPPER SEQ Em 002 HLL STEF 5EQ 9 MENU SPEED 2 Press PIP to aspirate sample Screen display 500 L move key to set the target volume to 450 L 19 a E SU ul Li vit vit STEP SEQ STEP SEQ C1 Yo C1 Yo MENU SPEED MENU SPEED 3 Press to proceed first dispenses 450 L Screen then display default of Vos is 450 L Press key to set the target to 400 L as follow EE c SL SO vis vis STEP SEQ STEP SEQ C1 y9 C1 y9 MENU SPEED MENU SPEED 4 Press to d
25. matically reduce times of aspirate if above calculation is violated Different model of smartpipette has different factory default setting as follow S100 VOLUME 2 L TIMES 5 S100f VOLUME 20 Lf TIMES 5 S300 VOLUME 20 Lf TIMES 5 1200 VOLUME 200 L TIMES 5 S5mL VOLUME 500 L TIMES 5 S10mL VOLUME 2mL TIMES 5 IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of ASPIRATE mode Select ASPIRATE mode set volume and times operation is ready 1 Immerse the tip perpendicular to liquid and press PIP liquid is aspirated in to the tip Screen display times for the rest of sampling 21 2 When first aspirate is completed remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid 3 Immerse the tip perpendicular to second liquid sample and press PIP the 2nd liquid sample is aspirated in to the tip Screen display times to be aspirated for the rest of liquid 4 Repeat the process until total volume is aspirated screen display the rest of times is 0 volume of total fill in 5 Press to dispense the sample 6 If required replace new tip for next operation GEL This mode is specially designed for gel pipetting Aspirate speed is adjustable dispense speed is set to be low to pr
26. n cycle 4 4 2 RPIPET mode Rpipet will fill extra volume when aspirating ES Dispense is done in 2 steps First step is SU ul to dispense required volume Second step een is to dispense the rest of volume This a3 mode is especially suitable to viscous HENU SPEED liquid Parameter set up VOLUME Value between valid range is available for set up Factory default setting is the maximum volume given by specification IN SPEEDO Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Refer to parameter set up on PIPETTE mode for detail description Operation of RPIPET mode Select RPIPET mode set volume and speed operation is ready 1 Immerse the tip perpendicular to liquid and press PIP liquid is aspirated into the tip 2 When aspirate is completed remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid Er 200 ul RPIPET L SPEED 3 Press to dispense desired volume There are still extra liquid in the tip Screen will display PIP as follow 13 4 Press PIP to clear the rest of liquid from the tip 5 If required replace the tip before next operation 4 4 3 STEPPER mode This mode fill in total required volume Lt Total required volume volume x times of SU ul dispense and dispense equal volume in ES number of times It is special suitab
27. n of liquid sample and further influence the result Calibration room with few air circulation temperature meter and humidity meter is recommended to ensure the accuracy Facilities 1 Analytical balance A suitable analytical balance is determined by the volume of the electronic pipette 26 Linearity and repeatability of Measurement the analytic of inaccuracy balance Volume of Reading of electronic analytical pipette balance 1 L 10 L 0 001mg 0 002mg lt 0 002mg 10 100 L 0 01mg 0 02mg lt 0 02mg 100 L 2 Pure water dehydrogenized water or distiller water qualified with ISO 3696 3 Shock and dust resistant area distant from heat source and sunlight Procedures To ensure accuracy we adopt 3 calibration points 10 measurements 1 calibration point as following procedures 1 Clean up pipette and check lubricate condition of piston 2 Ensure the pipette tips analytical balance distiller water and all the facilities are located in the same environment for more then 2 hours 3 Before calibration process pre rinse the tip by going through 5 cycles of aspirate dispense 4 Measure 100 50 and 10 of maximum volume given by the specification 10 times each point 5 Calculate the average value based on it calculate systematic error and random error based on formula specified in ISO 8655 6 Compare the results of measurements and ISO 8655 to determine whether configuration is furthermore required 2
28. pette is aspirated the operation will automatically switch from aspirate to dispense All you need to do is to press down to complete dispense 5 After 1 operation cycle aspirating and dispensing screen display PIP instruction Press to clean up the rest of liquid in the tip Make sure that you hear alarm 2 ticks before next operation cycle Noticef lt is recommended to use lower speed for this mode to ensure the best operation result 4 4 6 4special pipette modes SPECIAL has 4 special modes under this menu PIPETTE HMVIX This mode allows operator to mix sample after dispense Suitable for sampling and dilute sampling n mmmn SU ul SS uL x OS PIPETTE HIX PIPETTE H IX AQ C1 AQ HEHU SPEED HEHU SPEED Parameter set up PIPETTE VOLUME Value between valid ranges is available for set up MIX VOLE Value between valid ranges is available for set up MIX TIMES Times of mixing aspirating and dispensing cycle 1 time maximum setting value is 200 Counter will not display 3 digits 100 200 This parameter setting can only be done from SET PAREMETER menu 17 Different model of smartpipette has different factory default setting as follow S10 PIPETTE VOL 5 L MIX VOL 4 5 LI MIX TIMES 5 S100 PIPETTE VOL 50 LO MIX VOL 45 Lf MIX TIMES 5 S300f PIPETTE VOL 50 LI MIX VOL 45 U MIX TIMES 5 S1200 PIPETTE VOL 500 LI MIX VOL 450 LO MIX TIMES 5 S5mL PIPETTE VOL 1000 L MIX VOL 500 L MIX TIMES 5 S10mL
29. ric outlet Note 1 Color of battery state indications Blinking black or white state bar indicates power is being charged Blinking green state bar indicates power has been full charged 2 It is normal that temperature of battery will be higher when it is being charged 3 Excessive charge of the battery may damage the battery or shorten the battery life 4 Old battery may cause malfunction of the pipette It is recommended to replace battery block in every 2 years 4 2 Fitting and ejecting tips Fitting tips Make sure you select compatible tips The recommended fitting method is to insert pipette perpendicularly into the tip rotate the tip 180 degree left and right for firm fitting smartpipette is basically compatible to tips of major brand Notice Liquid should never enter the tip holder Eject the tip smartpipette incorporate ergonomic tip ejection design Gently push ejection button the tip will be ejected from the pipette 7 4 3 Getting start 4 3 1 Turn on and Stand by Turn On Smartpipette is turned on automatically when the battery block is inserted accordingly The screen will then display RONLABS smartpipette press in sequence After press PIP screen will display AUTOTEST piston diagnostic is on the working When self diagnostic is over screen should return to main menu or pipette mode Example of model S1200 is shown as follow ESS m SU PRESS AUTOTEST PIPETTE PIP
30. s device close to an environment with explosive gas Do not disassemble the product with abuse If the product does not function normally stop the operation and confirm troubleshooting chapter in this manual Operating temperature should maintain between 15 Cto40 C relative humidity should not exceed 80 Store electronic pipette battery charger and other accessories carefully 10 Instrument warranty This instrument is warranted to the original purchaser by Ronlabs Scientific to be free of defects in materials or workmanship for a period of 12 months from the date of purchase providing that is has been operated according to the instructions not abused or misused that the serial number has been removed and the instrument has not been disassembled other then procedures for normal maintenance No other warranty is expresses or implied including the warranty of merchantability Should the unit need to be returned the purchaser must pay insurance and shipping cost charges Ronlabs Scientific will assume the cost of returning the repaired unit to the purchaser This products packaging is capable of withstanding normal shipping hazards 44 11 Permissible errors RONLABS smartpipette Reference Systematic Model range Volume Stor 0 2 0 04 1 0 05 S10 0 2 10 5 0 08 10 0 10 5 0 40 10 0 35 S100 5 100 50 0 20 100 0 40 20 0 80 30 0 60 S300 20 300 150 1 00 300 1 20 100 2 00 120 0 50 1200 100 12
31. t When the value is highlighted in red and blinking press selection key will adjust value Then press left key to confirm key to exit and return to previous menu 4 3 3 Pipette Modes Press left button to enter menu highlight CHANGE MODE and OK to enter Press key to select target mode and OK to confirm m PIPETTE CS SET PARAMETER RPIPET PROGRAM LIST STEPPER O ul SETTING MIX x LOS CALIBRATE e MANUAL GTEPPER m SPECIAL ag OK MENU SPEED 4 3 4 Set up Parameter There are 2 ways to set up parameter On main screen Selection Left buttons are all you need to access quick setting Please refer to next chapter for detail description From SET PARAMETER Follow the steps underneath allow you to set up parameters easily Menu SET PARAMETER use selection key EXIT will return to previous screen OK to enter parameter setting Consequently parameter setting can be done with Selection Left and buttons Refer to following chapter for detail description CHANGE MODE Lae ESET PORIMETER Ven SETTING PIPETTE CALIBRATE SET VOLUHE 9 AQ HENU SPEED EDIT EXIT 4 4 Modes description 4 4 1 PIPETTE mode PIPETTE mode Is alSo known as automatic mode which aspirate and dispense 200 according to set up value This mode De incorporates auto purge function to ensure PIPETTE no retention on the tip is most common for 9 beginner MENU SPEED PARAMETER Se
32. t up VOLUME Value between valid range is available for set up Factory default setting is the maximum volume given in the specification IN SPEED Default setting is 9 Available range is 1 9 OUT SPEED Same as IN SPEED Maximum speed is 9 Setting Methods From main screen or SET PARAMETER is available Main Screen To increase volume Press selection up key to decrease volume Press selection down key Setting aspirate speed Press right button before aspirating Setting dispense speed Press button after aspirating SET PARAMETER 1 MENU SET PARAMETER 10 CHANGE MODE SET PARAMETER OU ul PROGRAM LIST u SETTING PIPETTE CALIBRATE A 9 HEHU SPEED 2 OK key EDIT Parameter is blinking and ready for set up man hana SET VOLUME AQ EDIT EXIT 3 Press key to change values and confirm with OK ET SET VOLUME AQ OK EXIT 4 Repeat above process to complete speed setting of aspirate and dispense m m nni nul SET IN SPEED SET OUT SPEED 6 FG EDIT EXIT EDIT EXIT 5 After parameter setting is completed press key SAVE AND EXIT is prompted YES to confirm YES blink YES to confirm 1 more time n 1 OO aL SAVE AND EXIT 6 YES NO 6 Press EXIT to return to main screen Notef f you wish not to save the change of parameter Press NO to abort and return to main screen Operation of PIPETTE mode Select PIPETTE mode se
33. t volume and speed operation is ready 1 Immerse the tip perpendicular to liquid and press PIPL liquid is aspirated into the tip 2 When aspirate is completed remove the tip from liquid gently touch the tip on edge of vessel to clean up excessive liquid mmn Pou PIPETTE C1 V9 MENU SPEED 3 Press to dispense liquid Alarm is designed to notice the completion of 1 operation cycle 4 If different type of liquid sample is aspirated new tip should be replaced to prevent contamination Notice Keep pressing key until tip is removed from the liquid will prevent re aspirate caused by piston relocation This function is designed to ensure the precision of small volume pipetting Note 1 During aspirating cycle do not dip the tip to the bottom of the vessel This way may influence the accuracy 2 After aspirate wait for 1 second sample volume lt 1000 L to 3 second sample volume gt 1000 L before removing the tip from liquid 3 If sample s viscosity or density is higher then water pre rinse 2 3 times before operation is recommended Pre rinsing consists of aspirating certain volume of liquid and then dispensing it back into the same vessel or waste 4 Tips should be immersed approximately 3mm for smaller models as S10 S100 S300 S1200 and 5mm for larger models 12 as S5mL S10mL 5 After 1 operation cycle aspirating and dispensing make sure that you hear alarm 2 ticks before next operatio
34. tting of dispense speed out speed in the same method 2 x 11 SET IN SPEED Pi AS EDIT EXIT Scroll down SAVE AND EXIT is prompted gt YES blinking for confirmation YES to confirm and return to ul 11 previous screen SAVE AND EXIT Pi lt A 9 YES NO Note Setting up program for P1 P6 can only be done from program list Main screen is not available for quick access programs Access program P1 P6 1 From previous screen in step 5 scroll to select ENTER OK to confirm 2 From main screen MENU PROGRAM LIST Pl ENTER as follow 25 i CHANGE MODE B SET PARAHETER 1266 PIPET SOC ul u SETTING PIPETTE CALIBRATE 9 MENU SPEED EXIT 1206 PIPET CHANGE MODE SET PARAMETER m SAVE EDIT SPEED 4 5 5 CALIBRATE smartpipette is factory calibrated with distiller water based on ISO 8655 standard Generally a new electronic pipette does not need calibration or configuration For special requirement liquid with significantly different gravities or temperature Calibration feature is available to achieve greater accuracy In addition if the device is frequently used for certain period of time calibration process is also recommended in every 3 months Calibration environment Calibration environment should be controlled with room temperature 15 30 C 0 5 C humidity 50 80 Higher level of humidity is preferred to prevent from instant evaporatio
35. ver Reassembly of the pipette is complete Reassemble procedures for S5mLf S10mL Use a pinch to place o ring E3f or F3 back to E4 orF4f 1 insert tip of piston E1 or F1 into E2 or F2 screw Ed into E27 or F20 El E2 i E4 L 1 2 3 2 Restore E or F5 and EQ orF2 press air vent with finger 39 and insert piston tip into piston holder Notice If air vent is not covered the piston may not be inserted into piston holder due to air leak Ae E2 E4 E4 I T Le Air vent T 1 2 3 Screw the lower section grey into the grip section white clockwise press down and hold the ejector button S1 and fit the hinge section E5 or F5 the reverse way of assemble procedures 1 2 40 4 Restore lower section of tip ejector there are 2 fitting notches on both E6l or F6 and E5 or F5 match the notches in position and pop one side in first and the other side later 5 Restore battery block into battery housing restore housing cover Reassembly of the pipette is complete 8 Maintenances 8 1 Cleaning and decontamination Smartpipette is designed for easy maintenance as regular maintenance will ensure the accuracy and precision in your daily operation It is especially important if the liquid you are working with is erosive solvent Notice Jlt is important to remove battery before you proceed with cleaning and decontamination Exterior Methods Wipe the e
36. xterior with soft cloth or lint free tissue impregnated with 60 of IPA water Then wipe the entire pipette again with stiller water Check if the exterior of pipette is clean and free of dust especially tip holder before every operation Leave the parts to dry by evaporation or wipe them within a clean soft cloth Interior Procedures 1 Disassemble all parts for cleaning according proper procedure 2 Wipe the exterior with soft cloth or lint free tissue impregnated with 60 of IPA water Then wipe the entire pipette again with stiller water 3 Generally o ring piton doesn t require special cleaning If dust or any particle is detected wipe out with clean soft cloth 4 Refresh a thin layer of lubricate on o ring and piston 5 Reassemble according the proper procedures Decontamination The handle is not autoclavable Only the bottom sections including tip ejector tip holder o ring connecting nut can be 41 autoclaved individually as following procedures Clean the parts especially tip holder Put the parts into autoclaving sack Autoclave at 121 C 1 bar for 20 minutes Check if the parts are dry before reassembling the pipette ON a ee I Set the pipette aside to stabilize for at least 2 hours smartpipette is produces with anti aging material With ultraviolet you can expose the pipette all day without disassemble 8 2 Easy maintenance Different level of maintenance will be required based on diff
37. y 43 84 Storage 43 9 Precautions and limitations 44 10 Warranty information 44 41 Technical data 45 2 Appendix 46 Correction factors for distiller water as a function of test temperature and air pressure Appendix 2 47 Accessories 1 Introduction smartpipette is the newest generation of electronic pipette utilizing air replacement concept to aspirate and dispense liquid smartpipette incorporates many breakthrough features such as comfortable design suitable for griping and operating on right or left hand Color screen for clear visualization frequent interval calibration to insure the linearity and accuracy of each single measurement wide range of operation modes to meet the needs of high end research purpose 5 common modes and 4 special modes enable all kind of operations demanded in labs Clear and simplified interface make it a user friendly instrument Cri his 601081 ssm s00 s000L 51 sm 110ml 2 Package contents Parts check list Electronic pipette Battery block Battery charger Lubricate USB cable User manual Warrantee card Please check the package contents immediately to ensure free of transportation damage or loss 3 Product descriptions 3 1 Screenf smartpipette Battery State Volume and units 1 Sate ul Piston Position Mode PIPETTE Cycle Counter C1 AQ Speed Menu MEHU SPEED Aspirate Dispense 3 2 Keypad Left smartpipette Menu Confirm
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