QMM Micromanifold - ALA Scientific Instruments
Contents
1. use of various sizes of tubing to be used with the Micromanifold without the necessity to get an exact match The silicone tube is glued to the quartz tubes with a cyanoacrylite glue Crazy Glue or Super Glue Should one come off it should be reattached with a similar product In order to insert a tube into the silicone you may first have to expand the tube slightly with the insertion of a large pin or other sharp object Always cut a straight square end on any tube to be inserted into a silicone tube or connector where possible Otherwise a beveled edge is easier to insert although it may present clogging problems so perhaps a compromise angle cut is best The inside diameter of the silicon connectors provided is about 100um Should this be smaller than the inside diameter of the tubing you are using to connect to the Quartz Micromanifold you may wish to remove the silicone connectors or use them to seal over the connecting tubing For example if the tubing you use has a larger ID than the OD of the quartz capillaries then the tubing can be placed over the capillaries but the connection will be leaky In this case the silicone connectors can be slipped over the interface of the tubing quartz capillary to form a seal When silicone connectors are glued in place on 100um ID tubing they may be removed with a small amount of acetone at the glue spot Be sure to remove all glue residue carefully so as not to clog the capillary Keep ace2. QMM Micromanifold User Manual ALA Scientific Instruments 1100 Shames Drive Wesibury NY 11590 Tel 516 997 5780 Fax 516 997 0528 Email sales alascience com www hittp Awww alascience com Introduction The quartz micromanifold consists of anywhere from 1 14 tubes Usually they are about 100um ID A single 200um tube is added to act as a reverse flow tube in order to suck the dead volume of the manifold clear or provide an input wash for the same purpose All specifications as to the number of tubes must be specified at the time of ordering The standard configuration of a QMM is a 100um ID tubes for solution delivery b one 200um tube for flushing the c dead space and a 100um tip approx 5mm long The tip front part of the manifold is removable a The output tube ID is usually 100um but other sizes are available The large part of it is en composed of silicon tubing which forms a tight seal over the multi tube cluster c The gap dead space in a the manifold can be adjusted by sliding the silicon up or down on the cluster The dead space can be calculated by using an equation to determine the area of the conical section where the base is about 1mm the top is about 250um and the length is the size of the gap Dead volumes are usually less than 1ul and typically between 50 and 100 nl Additional dead volume comes from the length of the single outlet tube Its volume can be calculated by using the formula f
3. er the cluster by pushing on the tip It can break and cause injury Do not use plyers or any other tools Simply insert the cluster and twist the cluster and the silicone part of the tip in opposite directions as you push the tip section over the cluster Keep in mind that the conical gap combined with the output tube represent the dead space in the system therefore the size of these parts should be kept as small as possible Due to the abrupt conical shape of the silicone it is possible to restrict the flow of a tube If this happens try to re adjust the silicone sleeve until the flow of all tubes are the same Be sure not to compress the tip too far over the cluster so that it blocks some of the tubes If you do not get flow pull the tip off slightly and try again Under normal use solutions should flow through the manifold under gravitational force A height of 1 2 meter is adequate Adding additional pressure of just a few PSI 10 20 kPa will increase flow significantly Flow rates will be affected by the length and diameter of the feed tubes Remove all air bubbles from feed tubes as they cause irritating compliance with regard to flow For those who wish to use the ALA FEP tubing Teflon Dupont see below for ordering information or we recommend PE 10 tubing Polyethylene for all connections Each tube of the Micromanifold has a piece of Silicone tubing attached to act as a link to other tubes The Silicone tube permits the
4. ith a syringe under pressure to free it up The same method should be used for the tip if it becomes clogged To find a clog 1 2 Remove the tip Determine that all the tubes on the QMM flow with the tip removed This is accomplished by attaching an empty syringe 10ml to each tube on the QMM placing the output of the QMM in water and applying pressure to the syringe You should see air bubbles coming out of the QMM output Repeat this for each tube including the tip If there is no air bubbles coming out of any one of the tubes then that tube is clogged To unclog a tube 1 2 3 Use a syringe 10ml filled with a flush solution such as distilled water or isopropyl alcohol and attach it to the clogged tube Place the output of the QMM in a container filled with the flush solution Push the flush solution through the tube If the flush solution does not flow through the tube push and withdraw the syringe repeatedly until the clog is broken Repeat for each clogged tube Note If the QMM tube can not be unclogged using the procedure above you can try the following steps 1 2 Place the QMM in an ultrasonic cleaner Repeat unclogging procedure In unclogging procedure use boiling water to soak the QMM while applying pressure on the syringe
5. or the area of a cylinder with a diameter of 100um and a length of however long it is cut It may be cut as short as is needed after cutting the tip should be polished using 600 grade sandpaper only Cut with wire cutters and always shield eyes Tubes are made of quartz glass coated with polyamide They are rather flexible and durable The tip should not break even if bumped repeatedly against the bottom of a petri dish Tubes are held together with a cyanoacrylite glue The quartz glass is very durable and somewhat slippery to a variety of solutions including those containing proteins and lipids Clogging should not be a problem but care should be taken to be sure that filtered solutions are used If a tube should become clogged it can be blown out with air liquid pressure The quartz tubes can withstand pressures of several thousand PSI Fig A INSTALLATION OF THE TIP ON THE MICROMANIFOLD Push tube cluster into silicone sleeve of tip It is necessary to work it in carefully The silicone tube is cut with a bevel to ease insertion TIP OUTPUT TUBE SILICONE SLEEVE CLUSTER After the tip of the cluster is in carefully push the sleeve over it Work the cluster in until just a small gap appears It will be conical in shape This space should be kept small but be carefull not to push the cluster in so that it remains pressing against the output tube CONICAL GAP Cautions Do not attempt to push the tip section ov
6. tone away from the front end of the Micromanifold If your tubing forms a tight seal with the Quartz capillary then there is no need to use the silicone connector Quartz tubes of 100m ID can sometimes be mated to PE 10 tubing Polyethylene Tubing 10 Clay Adams Co now a division of Baxter which is available from Thomas Scientific through their catalog Teflon tubing and silicone connectors are available from ALA or your distributor 200um ID tubing can usually be mated to PE 20 also available from the above sources Daily Maintenance At the end of each day you may wish to flush out the tubes of the Micromanifold Some may want to disinfect these parts as well You can use any bacteriacide you wish Just do not use strong acids strong bases or acetone To flush out each tube of the QMM attach a syringe 10ml containing a flush solution to each tube and pushing the solution through the QMM and out the tip It is a good idea to keep the tip of the Micromanifold submerged to prevent it from drying out The same would apply to the rest of the cluster if the tip was removed If you blow out all lines with air the above is not necessary If a tube should become clogged follow the cleaning procedure in the next section Micromanifold Cleaning Procedure The Micromanifold should never be heat sterilized but can be autoclaved to 120 C If a tube should become clogged it should be isolated and distilled water should be worked in w
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