User`s Manual - INHECO Industrial Heating & Cooling GmbH
Contents
1. CONVENTIONAL USAGE WHO IS PERMITTED TO OPERATE THIS UNIT SERVICING THE INCUBATOR TECHNICAL ALTERATIONS MALFUNCTIONS NAME PLATES AND LABELS OPERATING THE INCUBATOR SHAKER DWP SCOPE OF SUPPLY INITIAL OPERATION USB COMMUNICATION COMMUNICATION OF INCUBATORS TO THE USB HOST PC COMMANDS TECHNICAL SPECIFICATIONS TECHNICAL DATA SHAKER FUNCTIONALITY THERMAL FUNCTIONALITY MODULE DIMENSIONS WEIGHT 5 4 1 DIMENSIONS AND WEIGHT 5 4 2 DRAWER SPECIFICATIONS BA IncubatorShakerDWP 10 10 11 11 11 13 13 13 14 14 15 24 24 27 27 27 28 29 31 31 32 6 1 6 2 10 11 12 FINAL QS TESTS DRAWER TEST PERFORMANCE TEST CALIBRATION AND ADJUSTMENT AUTOMATICALLY SELF TEST AQS DETAILED DESCRIPTION OF THE ERROR CODES TYPICAL PROCESS EXAMPLE WARRANTY POWER SUPPLIES DECLARATION OF CONFORMITY BA IncubatorShakerDWP 32 32 32 32 32 32 34 34 35 38 1 Explanation of Symbols and Abbreviations A possible danger leading to serious bodily harm is being pointed out to you Caution hot surface A possible danger leading to less serious bodily harm is being pointed out to you This signal also warns you of tangible damage A A A possibly dangerous situation leading to material damage is being pointed out to you Important This sign refers to useful information as to installation etc e Bullet points refer to enumeration gt These arrows indicate instructions The squares refer to2. procedures running automatically and the results to be achieved BA IncubatorShakerDWP 7 1 1 Abbreviations The document uses the following terms CC Communication controller Controller Microprocessor with on chip peripheral CRC cyclic redundancy Check FCS Firmware Command Set Heater Same as Temperature Controller IVD D In vitro diagnostic directive MP Micro Plate PCR Polymerase Chain Reaction PWM Pulse Width Modulation Shaker Controller for shaker regulation TBC To Be Continued Temperature Controller Controller for heat regulation USB Universal Serial Bus CE Conformity mark NRTL Nationally Recognized Testing Laboratory DLL Dynamic Link Library PC Personal Computer EU European union SBS Society for Biomolecular Screening DWP Deepwell Plate IVD In vitro diagnostics FDA Food and Drug Administration DIP Dual in line package BA IncubatorShakerDWP 2 Safety Instructions Avoid accidents Always make sure that the unit is disconnected from the power supply system during any installation process Electric Shock Hazard You can suffer an electric shock if the unit is not connected properly or if you did not disconnect the unit from the power supply system before opening the housing Please observe the following measures in order to avoid muscle convulsions burns unconsciousness apnea or even death Do not work with open housing when unit is connected to the power supply sys
3. to 1 2 X 1 0 to 1 2 20 or or Y 2 1 0 to 1 2 Y 1 0 to 1 2 Round Phase shift 90 Shaker Load 100g Shaker amplitude mm Shaker Speed Hz Single Device Triple Stack 10 X 1 0 to 2 2 X 1 0 to 1 9 Y 1 0 to 2 2 Y 1 0 to 1 9 15 X 1 0 to 2 7 X 1 0 to 2 4 Y 1 0 to 2 7 Y 1 0 to 2 4 20 X 1 0 to 1 2 X 1 0 to 1 2 Y 1 0 to 1 2 Y 1 0 to 1 2 shaker table door 5 3 Thermal Functionality Parameter Value Temperature range Room Temperature plus 5K to 80 C 176 F Accuracy absolute The accuracy is defined as the difference between the target temperature of ambient the heating plate and the average of the measured temperature on the temperature stability heating plate The heating plate temperature is measured at 5 positions on 1K the heating plate according to the Special INHECO measurement head PM 0105 The 5 measurement positions are according to the Plate positions A1 BA IncubatorShakerDWP H1 E6 A12 and H12 Target Temp Validation Tool Accuracy 37 C 98 6 F 1 60 C 140 F 15 80 C 176 F 2 Uniformity measured in well Uniformity is defined by the maximum difference between lowest and highest temperature according to a Nunc 96 Round Bottom A1 A12 E7 H12 Target Temp Validation Tool Uniformity C 37 C 98 6 F 0 8 60 C 140 F 12 Evaporation
4. you are able to open four COM ports Just click at Open COM on the four tabs For the implementation it is important to consider different setup conditions Some devices may be already connected to the USB green LED is on some may not green LED is blinking at 1 Hz The blinking of the LED stops only after the first message with the correct device ID is sent to the Incubator device Therefore you need to send at least two messages to bring all devices into the same state The reason for that is that slave devices have to register themselves to the master device within a certain time frame This happens after the first correct USB message to the master was sent IYDD Incubator Shaker Control 10 xl Find Incubator Shakers Com Ports At Port COM are 1 Incubator s with Device ID 1 and Serialnumber s TTO5 At Port COME are 2 Incubator s with Device ID 2 and Serialnumber s TT27 TT28 IncShak C va 22 04 2007 Picture 20 Application Example The COM ports may change therefore pleas use the device ID to have a unique identification of a stack Parallel communication to different COM ports is possible Parallel communication with different members of one stack is forbidden and may result in errors BA IncubatorShakerDWP 26 4 5 Commands All commands for the integration of the single Incubator are described in the document Incubator FCS Document NR 900334 XXX 5 Technical Specifications 5 1 Technical Data Tempe
5. ge frequencies etc Optional restart shaking Stop shaking and heating Open all doors 11 Warranty 2 Years from date of shipment Any damage by abuse or caused by operation different from this instruction is not covered BA IncubatorShakerDWP 34 12 Power Supplies The Power Supplies can be ordered separately at INHECO Picture 22 Power Supply built for 1 and more incubators Power Supply built for 1 and more incubators AC Input 100 240V 2 5A 50 60 Hz DC Output 24V 7 5A Part 2400121 Picture 23 U K Power Supply Cord set Part 1004214 BA IncubatorShakerDWP 35 Picture 24 European Power Supply Cord set Part 1004184 Picture 25 North American Power Supply Cord set Part 1004185 BA IncubatorShakerDWP 36 Table 2 Version Management VERSION NO RELEASE DATE CHANGES TO PRIOR VERSION May2012 EEE O BA IncubatorShakerDWP 37 1 Declaration of Conformity INHECO GmbH 82152 Martinsried Germany Declares that the following product Incubator Shaker DWP Meets the essential requirements of the following European Union Directive s using the relevant section s of the normalized standards and related documents shown Standard and Date of Issue IEC 61010 1 2001 2 Edition Safety requirement for electrical EN 61010 1 2001 2nd Edition equipment for measurement control and laboratory use EN 61010 2 010 2004 Particular requirements for laborator
6. he microplate will be clamped on the drawer When the drawer reaches its end position inside the Incubator Shaker the microplate will be sinking on the heat plate for best thermal contact You can hear a short shaking noise to ensure that the microplate is sinking on the heat plate but only when the microplate is detected correctly from the device The heat plate has three integrated sensors for measuring the temperature The temperature of the sensors is averaged BA IncubatorShakerDWP 20 Electrical Details General The device needs an electrical power supply that provides a stable 24Vdc voltage The power consumption for one device is max 2 7 Ampere 65 Watt If more devices are stacked the power supply must be able to deliver the resulting power consumption The polarisation at the power connector at the back side is shown in the following picture The red wire symbols the plus pole and the black the minus pole To communicate with the device a computer with an USB Connection is necessary The USB Connector is placed at the back side see blue mark at picture 8 connection O Master Device Q Picture 8 Standard connection for one device BA IncubatorShakerDWP 21 Address Switch A six pole DIP Switch can be found at the back side Only the switches 1 to 4 are used for setting the address These switches are for identification if more than one single device or stack is connected to the PC The default address
7. in unsealed Micro Plates Incubator temperature 37 C 98 6 F max 10 loss of liquid in 4 h deionized water room temperature 24 26 75 2 78 8 F rel humidity 40 50 altitude at 410 m over Sea level If there are problems with extreme condensation the functionality of the upper heating foil might be no longer given Check the performance of the upper heating foil by reading out the temperature with the commands RAV5 RAV6 RAV7 RAV8 at ambient temperature Record the values Heat the Incubator five minutes at 80 C and then read out the values again The values should be decreased by four points if the upper heating foil is working within specifications Heatup time Start temperature 15 6 C 60 F Target temperature 37 C 98 6 F Heat up time max 100 min Volume 1 5ml per well The target temperature is reached when all sensors are in the tolerance band of 37 C 1 C 98 6 F 3 6 F Measured with 1 NUNC 96 Deep Well Plate BA IncubatorShakerDWP 30 5 4 Module Dimensions Weight X Module Drawer Picture 21 Dimensions 5 4 1 Dimensions and Weight Parameter Value Dimension in Xwodule 149 mm ER 1 268 5 mm without connector cables Dimension in Yvodule 2 298 5 mm with connector cables Dimension in Zodule without pin 139 mm Open drawer incl door Y Drawer 209 mm Dimension of thermal chamber in Zchamber 49 mm Weight without cables 5 4 kg BA I
8. inheco industrial heating amp cooling INHECO stackable devices Incubator Shaker DWP Part No 7300009 Document Nr 900239 000 User s Manual Important The device has to be in a level position at all times it may not be tilted or turned upside down especially not when the transportation screws are not in place Revision level V1 0 Draft Version for Details Contact INHECO May 2012 BA IncubatorShakerDWP 1 INHECO Industrial Heating and Cooling reserves the right to modify their products for quality improvement Please note that such modifications may not be documented in this manual This manual and the information herein have been assembled with due diligence INHECO does not assume liability for any misprints or cases of damage resulting from misprints in this manual If there are any uncertainties please feel free to contact us The brand and product names within this manual are registered trademarks and belong to the respective titleholders BA IncubatorShakerDWP 2 How to contact us INHECO 82152 Martinsried Germany Telephone Sales 089 899 593 101 Telephone Technical Hotline 089 899 593 104 Fax 089 899 593 499 E Mail Sales sales inheco com E mail Technical Hotline techhotline inheco com Website www inheco com This manual belongs to Type Serial No Year of Manufacturing Order Confirming No PRODUCT SERVICE To be filled in by customer Inventory No Place of installati
9. iption of the Error Codes The error codes embedded into the message reply are mainly meant to give the user additional information about the status of the device The reply to every command ends always with the following three hex data 0xBO device ID BA IncubatorShakerDWP 32 This ensures that it is a reply from the device with this device ID 0x20 error code The following table 1 describes the error codes see also Firmware Command Set Table 1 error codes Code Error message Detailed description 0 Message Ok Normal return message 1 Reset detected Power on software or watchdog reset Three kinds of resets are possible i Power on reset gt after the first ordinary message reply after power on this error code will be send This is a correct behaviour ii Software reset gt after the command SRS the green led in front of the device blinks shortly The software reset brings the device into the same state after power on Therefore also here this error code will be send iii Watchdog reset gt if the firmware runs into trouble in most imaginable situations a watchdog reset occurs This error code will be send Please inform the INHECO Tec hotline in such a case 2 Invalid command The Command does not exist for this device This flag is set if the command is completely unknown E G if a shaker command has been sent to an Incubator without shaker function 3 Invalid o
10. is 2 The recognition of the device stack depends on the switches position as shown in picture 9 EFT ATTI i9 is r e e ol i9 s r e e i Picture 9 Address Switch BA IncubatorShakerDWP N 12 13 14 15 Connecting Devices Two devices are operated with one power supply and controlled via one USB interface the 15 pole SUB D Connectors on the back side are used The following pictures show how the devices have to be connected When placing the device at its desired position make sure that the fan on the back side has at least 3 cm space to the wall to ensure proper airflow Picture 10 two connected devices o 654321 USB O EE Ded slave connection addressing O Slave Device os mo bread ojo slave connection Master Device O O BA IncubatorShakerDWP 23 4 3 USB Communication Hard and software features of the Incubator USB virtual COM port are specified in this section Remark The USB is not optimized for secure real time data transfer Therefore all communication is secured by a cyclic redundancy checksum crc If the communication between the PC and the MTC does often fail or results in timeouts in most cases the PC is causing the problem Therefore we recommend for a robust communication the following 1 Other devices connected to the USB
11. locally or at INHECO s Shut down and Disposal The unit is to be disposed in accordance with the environmental directives in effect in the respective country Caution EG The Incubator Shaker may only be operated in an upright and level position Important The Incubator Shaker unit must not be stored below 10 C BA IncubatorShakerDWP 12 3 5 Technical Alterations e Do not alter the product For safety reasons no technical changes to this unit are allowed by unauthorized persons Any modification or change which is not approved by the manufacturer in written form leads to loss of warranty CE e original parts are especially designed for the Incubator Shaker Parts provided by other suppliers are not tested and therefore not approved by INHECO Using such parts can impair the functionality of the unit and will lead to loss of warranty For damages which may occur due to the usage of non original parts or non approved modifications or repairs liability is excluded by INHECO Important 3 6 Malfunctions Report occurring malfunctions immediately to the responsible person named on page 3 of this manual Ensure that the unit is secured against violation and misuse Before the initial operation dismantled safety relevant parts have to be mounted and checked 3 7 Name Plates and Labels Please check all name plates and labels and ensure that their legibility is maintained at all times during the life time of this uni
12. might have an influence on the communication stability of the incubator Use a simple workstation PC The stability of the communication has to be verified with every PC Windows 7 and Vista seems to be more stable than Windows XP Do not activate the automated update from Windows ARON 4 4 Communication of Incubators to the USB Host PC Our recommendation for Incubator Shakers is to use it in three floor stacks For details see section 4 2 For the example on hand we want to use a more complicated configuration Five devices daisy chained with the D Sub D connectors Two power supplies are needed one for two and one for three devices gt Two devices one stack Onesingle device gt A second single device The LED indicates the status of the module e Power on and device is initialised gt gt LED on e Firmware download gt gt LED is blinking ca 3 5 Hz e USB Communication not present gt gt LED is blinking ca 1 Hz Details shown in the picture below BA IncubatorShakerDWP 24 Incubator with serial number TTxy Picture 18 Interconnection The starting basis is as follows USB drivers installed on the PC The PC is connected to the bottom Incubator of a stack via USB The INHECO tool is installed on the PC All COM ports are closed All Incubators are daisy chained power is switched on on and they are connected to the PC E On D After pushing the button Find Incubators COM Ports all COM P
13. ncubatorShakerDWP 31 5 4 2 Drawer Specifications Parameter Value Accuracy of the end position of the drawer in x y and z drawer open single unit all parts at 0 30 mm specified ambient temperature Maximum Drawer load 0 25 kg Open drawer resistance for a force in Z axis 10N without plastic deformation 6 Final QS Tests All units which leave INHECO have to pass through three semi automatic test procedures 6 1 Drawer Test The device must pass at least 100 drawer cycles with correct Labware detection without error 6 2 Performance Test Finally all important functions are tested and the self test AQS see chapter 8 must run without error 7 Calibration and Adjustment The adjustment of the shaker and or the calibration of the temperature are done at INHECO Settings are stored into the devices EEPROMS and redundant in an INHECO Database A redundant check of all data is done 8 Automatically self test AQS The automatically self test AQS is performing several tests to ensure proper functionality of the device After the tests are finished the device generates an error code see Firmware Command Set These tests are Drawer Test Checks opening and closing of the drawer Shaker Test Tests timing amplitudes and frequencies of different settings Temperature Test Tests timing and homogeneity of the heating procedure including boost gt Duration 3 min 30 sec 9 Detailed descr
14. on BA IncubatorShakerDWP Important Notes Read this manual carefully before using INHECO stackable devices In the following the names of the stackable devices Incubator Shaker or Incubator are used They represent one device of the family of stackable INHECO devices Liability does not apply for mishandling the unit This manual is part of the Incubator Shaker and must be e retained until the Incubator Shaker is disposed of e passed on to the new user when the Incubator Shaker is sold or lent Please contact the manufacturer in case you do not understand something within this manual Your opinion about this manual provides us with valuable insights on how we can serve you better Please do not hesitate to direct your comments to us using the address or the phone numbers on page 3 Please read the safety instructions very carefully They must be understood and observed in order to ensure safe handling of the unit Missing or insufficient knowledge of the manual leads to loss of liability against INHECO The operator should therefore ask for an instruction confirmation from the manufacturer to ensure proper handling of the device BA IncubatorShakerDWP 4 CONTENT 1 1 1 2 3 3 1 3 2 3 3 3 4 3 5 3 6 3 7 4 4 1 4 2 4 3 4 4 4 5 5 5 1 5 2 5 3 5 4 EXPLANATION SYMBOLS AND ABBREVIATIONS ABBREVIATIONS SAFETY INSTRUCTIONS OPERATING INSTRUCTIONS ABSTRACT
15. orts will be scanned and some commands of the Firmware Command Set will be automatically executed to determine the configuration The results will be written into the textbox of the tool and the combo boxes for COM port and device ID will be set respectively In more detail 6 Find out all available COM ports 7 Open next available COM port If none go to 18 8 Send an arbitrary command for synchronisation and announcement 9 Ifnoreply gt Close COM port and go to 7 10 Else important Look if a device ID error is present and look at the reply this is working since firmware version CC 3 17 11 gt Device ID of that stack master device 1 If you have used for example a USB Bluetooth adapter on your PC Windows sometimes creates lots of COM ports for this purpose If you plan to use that PC to control Incubators we recommend deleting all these COM ports first BA IncubatorShakerDWP 25 12 Send RDAO 1 to the master on this COM Port with the device ID found 13 gt Number of floors Slave devices 14 Send RFV2 to the master and all its slave devices take care of the floor ID T00 gt T01 gt T02 up to the number of slave devices 15 Store serial numbers COM port name number of devices and device ID 16 Close the COM port 17 Goto 7 18 Write the results to the textbox and fill the combo boxes of device ID and COM port name and the tabs accordingly 19 Finished see picture 16 for the result Now
16. perand Parameter out of range see command set This error code will be reported if the Parameter of a correct command is out of range E G SAX307 4 Protocol error USB Protocol error Wrong message length or CRC The USB Message is secured by a cyclic redundancy check at the last byte of a message If the last byte is incorrect or missing this error code will be reported 5 Timeout from device Device did not answer within the expected time frame This error must be generated by the PC application 6 Device ID error The device ID can be changed at the dip switch on the rear side of the device If the message one sends to this device has the wrong device ID this error is replied 7 Device not initialized Command requires prior initialization of a sub system E g It is not possible to open the drawer or to shake the Labware of a non initialised device 8 Command not executable Parameter or device condition prohibits execution The typical example is that no Labware has been detected and the user tries to shake using the command ASE1 9 Drawer not in end position The drawer is in an unknown position generated by RDS E G the door is blocked 10 Unexpected Labware Status A Labware is detected on AOD after a power up or after an undefined drawer status 11 Reserved gt 12 Reserved s 13 Drawer DWP not perfectly closed NTC resistor not connected check drawer 14 Floor ID error Device is not registered in the master E G A command has been send
17. rature range Tolerable relative humidity ambient Room Temperature plus 5K to 80 C 176 F 10 80 relative non condensing at 30 C 86 F or below Maximum load of the shaker platform Shaker frequency range Maximum shaking amplitude 0 25 kg 6 6 Hz to 30 Hz 400 rpm to 1800 rpm 3 mm difference between the maxima of devices in one stack BA IncubatorShakerDWP Maximum two due to interaction during shaking 2T 5 2 Shaker Functionality Parameter Amplitude range 0 0 3 0 mm For detailed shaker performance see chapter below Speed range 6 6 30 0 Hz For detailed shaker performance see chapter below Speed precision 2 2 Load weight of plate and liquid and adapter 40 250g For detailed shaker performance see chapter below Supervision speed and amplitude closed loop controller Shaker movement Shaker movement North South East West Round Eight Amplitude accuracy 20 of the target amplitude Phase shift accuracy 11 validated for the Heating Plate movement average value over 10 cycles BA IncubatorShakerDWP 28 Single Axis North South and East West Shaker Load 100g Shaker amplitude mm Shaker Speed Hz Single Device Triple Stack X 1 0 to 2 2 X 1 0 to 1 9 10 or or Y 1 0 to 2 2 Y 1 0 to 1 9 X 1 0 to 2 7 X 1 0 to 2 4 15 or or Y 1 0 1027 Y 1 0 to 2 4 X 1 0
18. routines guarantee a safe precise and fast processing of probes The Incubator Shaker is a state of the art device for incubating SBS listed micro plates including flat bottom and PCR with adapter plate Service support is guaranteed through INHECO BA IncubatorShakerDWP 10 3 2 Conventional Usage The Incubator Shaker is a state of the art device and complies with today s standards The manufacturer attached much importance to the user s safety The following rules apply to the user e Rules of accident prevention e General rules for technical safety e EU and other country specific directives The conventional usage contains the usage according to the manual You must be able to disconnect the main power supply to the instrument immediately if necessary An unimpeded air supply must be ensured to avoid injuries to persons and or damage to the unit Do not operate the Incubator Shaker DWP above the maximum ambient temperature to prevent the Incubator Shaker DWP from damage Ensure that there is a minimum of at least 8cm 4 inches free space at the rear ventilation openings The free space at the lower ventilation openings are accurately defined 3mm by the positioning pins 3 3 Who is permitted to operate this unit Only instructed and skilled persons are permitted to operate this unit Only specialized staff is allowed to make any amendments to the operating menu 4 Servicing the Incubator Run the Command AQS eve
19. ry 100h see Chapter 8 Temperature calibration may be checked and repeated regularly Every 15000 operating hours or three years whatever occurs first an authorized INHECO service technician should be check the device Calibrate INHECO recommends calibrating the Incubator device annually BA IncubatorShakerDWP 11 Cleaning Before cleaning the Incubator Shaker DWP disconnect the power and make sure that the temperature at the heating plate is below 50 C The contact surface should be cleaned regularly to ensure optimum heat transfer to the disposable Always clean the contact surface if there has been a spillage Use the cloth dipped in 50 50 water isopropanol solution and make sure that no deposits are left on the surface Care should be exercised to prevent water from running inside the unit Do not use aggressive cleaning fluids such as acetone or abrasive cleaners Please check with INHECO any cleaning method that is not mentioned in it his paragraph to prevent damage to the Incubator Shaker DWP Decontamination The most common decontamination method is by fumigation with formaldehyde or ethylene oxide gas The surface decontamination can include a wipe down of the housing surface A solution of 7096 alcohol should be used where effective for target organisms The use of Bleach 596 1296 or Microside SQ is also possible Repair The Incubator Shaker may only be repaired by authorized INHECO service technicians either
20. t Replace all name plates and labels if their legibility is no longer ensured BA IncubatorShakerDWP 13 4 Operating the Incubator Shaker DWP 4 1 Scope of Supply Before initial operation make sure that the shipment of your unit is complete and no parts are damaged The following components should be included in each shipment Incubator Shaker unit USB Cable between Incubator Shaker and PC 15 pole SUB D COM Cable CD or USB Stick containing Manual and Software ONS The Power Supply and Power Cable are not part of the scope of Supply they have to be ordered separately see chapter 12 Picture 2 Scope of supply BA IncubatorShakerDWP 14 4 2 Initial Operation Transportation Attention Do not turn Upside down Picture 4 Transportation Lock at the bottom side BA IncubatorShakerDWP 15 D Assembling disassembling of transportation lock Turn Incubator Shaker DWP slowly to the right Attention Cover side in direction of assembler like in picture Move A Picture 5 Assembling disassembling of transportation lock 3 pieces BA IncubatorShakerDWP 16 Turn Incubator Shaker DWP slowly to the left Attention Cover side in direction of assembler like in picture Picture 5 1 Assembling disassembling of transportation lock 3 pieces BA IncubatorShakerDWP After unpacking the device the two red screws transportation lock at the bottom side have to be unscrewed without til
21. tem Always switch off the unit before implementing any alterations The power supply is operated at a voltage of up to AC 240V Burn Hazard You can burn your skin when touching the inner parts and especially the microplate contact surface and disposables These parts can reach up to 85 C 185 F Do not use materials that are not sufficiently temperature resistant up to 110 C 230 F Explosive flammable and volatile materials may not be heated in the Incubator Shaker at the risk of explosions BA IncubatorShakerDWP 9 3 Operating Instructions 3 1 Abstract INHECO s compact single position Incubators are high performance plug amp play devices for the use on automated robotic platforms or as standalone units They can either be used as single devices or stacked in a tower In addition multiple towers or single devices can be controlled parallel from one host PC via USB The combination of different devices of the Incubator family in one tower is possible Picture 1 Stacked Devices The patented shaker design allows shaking of several different shaking patterns with high precision in frequency and amplitude The Incubators have built in safety features which enable them to be used in IVD and FDA applications Technical Specification chapter 5 The Temperature can be reported during processing to have a maximum of control Precise temperature and shaker control USB interface plate loading sensors and several self test
22. tes The Labware detection is optimized for Nunc Plates If different plates are used the user has to assure that the Labware detection works well with these plates too gt Light master plates are better than dark ones Master plates with matt surface are better than shiny ones gt Attaching an adapted tape on the left side of the master plate improves the success rate of the Labware detection With the command ASE1 the incubator shaker is unable to shake without detected Labware The command ASEND has been added to enable shaking without detected Labware We do not recommend using it The command ASE1 is preferred It ensures that the Labware is correctly placed on the shaker and performs within the specifications The bottom of the microplates must be flat to ensure uniform heat exchange If the customer prefers plates with different shaped bottom a special adapter is needed INHECO offers to design and manufacture a customized adapter The devices can measure the temperature at the top of the heat plate only The temperature of the liquid in the microplate differs from this Therefore a heater offset must be defined and set before the temperatures in the liquid samples are correct see also command SHO After Cmd AID for Initialization is send to the Incubator Shaker the door will close if it was open If the door was already closed a short buzzing sound can be heard When a microplate is put on the drawer and the door is being closed t
23. ting or turning the device upside down Turning the unit upside down might require a renewal of the shaker drive calibration Keep the screws for later transportation purposes To install the transportation lock back again don t turn the two screws too hard just finger tight Don t use a screwdriver This could damage mechanical parts inside the device Do not dispose the original package as it might be needed for further transportation Important The device has to be in a level position at all times it may not be tilted or turned upside down especially not when the transportation screws are not in place Picture 6 red Screws BA IncubatorShakerDWP Mechanical Integration Place the device on an appropriate plate for a firm and even stand Use the position pins on the bottom side for exact positioning of the device stack Important It is not advisable to stack more than three devices on top of each other without additional mechanical fixation Contact INHECO for more details Important The plate device has to be in balance for proper shaker performance Important It is not advisable to stack more than one device on top of each other without additional mechanical fixation Contact INHECO for more details With this method it is possible to connect a maximum of two devices one Master and one Slave BA IncubatorShakerDWP 19 8 Important The devices are built for the use with either deep well plates or micropla
24. to the slave device with ID 2 but only one slave is connected to the master 15 Timeout sub device The error message is from this device The sub devices heater or shaker did not answer within the expected time frame The Incubator Shaker has three uCs The heater and the shaker controller work as a kind of sub devices of the communication controller If the inter uC communication is corrupted this error code will be reported Most error codes can be used for a PC lab automation application to generate its own error codes or to change automatically its operation status if the device ID error is reported the device ID must be changed Ox60 End of reply BA IncubatorShakerDWP 33 10 Typical process example A typical cycle for a process should contain the following steps VVVVVVVVVVVVVVVVV VV Scan COM Ports Find device ID slaves and serial number of all connected devices Check the number of drawer cycles and the firmware versions of all devices Initialize all devices Open doors simultaneous or sequential Put in the Labware and the adapter if necessary Set target temperature for each Incubator Set heater offset according to the used type of micro plate Set frequencies amplitudes and phase shift for all devices Close the doors Start shaking Start heating Check cyclic temperatures frequencies amplitudes and the error flags Optional change target temperature Optional restart heating Optional chan
25. y equipment for the heating of materials EN 61000 3 2 Electrical equipment for measurement EN 61000 3 3 control and laboratory use EMC requirements EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements This product is in complies with the essential requirements of the Low Voltage Directive 73 23 EWG and EMC directive 2004 108 EC when used for its intended purpose Place Martinsried Munich Date May 2012 Signature Name Position Ginter Tenzler Managing Director BA IncubatorShakerDWP 38
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