StaRRcol manual ENG
Contents
1. Key ENTER or BREAK to start concentration measurements 7 1 2 Measurements with sample or reagents blank When a reagents blank 15 needed place the reagents blank into the measure unit and press blank During all the next sample measurements the reagents blank will be taken into account By measurements using a sample blank the above described instruction should be also carried out but instead the reagents blank a sample blank should be performed Performing of the samples place them in a sequence into the StaRRcol and wait 2 seconds and read the display or press PRINT StaRRcol Manual Page 23 Version 1 10 mechatronics StaRRcol functions 7 2 Accuracy and display The StaRRcol has a maximum accuracy of 12 bit and a maximum of 5 digits can be displayed including the decimals The displayed range is in between 1000 tot 32768 Automatic scaling is performed Possible scaling 1 000 32 768 10 00 327 68 100 0 3276 8 1000 32768 Whenever a reading is out of range the message overflow will be displayed The modes absorbency transmission and concentration will adjust the decimals automatically Transmission 0 00 9 99 10 0 99 9 en 100 Absorption 0 000 4 096 300 3 796 Note For concentration calculations a factor of maximum 4 digits can be entered Concentration 0 001 9 999 00 01 99 99 000 1 999 9 0001 9999 Manual altering the displayed decimals will not alter2. rue ee ee en ee ee 9 3 SAMPLE MEASUREUNIT 2 2 endete 11 A MEASURING METHODS anne 13 5 INSTALLATION Zee ee ee er 15 5 1 StaRRcol Initialising procedure 17 O STARRCOL DESIGN erat 19 1 STARRCOL FUNCTIONS See ee 21 7 1 Routine 23 7 1 1 Measurements by using a 23 7 1 2 Measurements with sample or reagents 23 25 1 2 1 source replacement use 26 7 2 2 Checking the light 27 8 ee er 29 9 SPECIFICATION 2 ee 31 Gl cs MM 35 9 2 StaRRcol Current Output 4 20 mAmp Optional 37 la DN oa 37 2 2 819 Dee nr ee 37 10 2 eee ee o 39 StaRRcol Manual Page 5 Version 1 10 mechatronics Introduction 1 INTRODUCTION The StaRRcol is a micro processor controlled filter photometer Due to application of modern electronics micro processor controlled and high quality Kohler optics the StaRRcol is very easily to operate and a reliable photo meter Low as well as high range absorption can be measured with high accuracy The user s interface consists of a keyboard a 16 character back lighted LCD display with large characters and a
3. 60 mm The standard light source and optics give a homogeneous lighted rectangular surface of 3 x 9 mm other surfaces possible at request StaRRcol Manual Page 11 Version 1 10 mechatronics Measuring methods 4 MEASURING METHODS The StaRRcol has been developed to measure sample concentrations in a homogeneous solution The intensity of the remaining light is detected after it passed through the sample The wavelength of the light source is set by interference filters These filters can be combined if needed by so called cut off filters The standard filters have a bandwidth of 8 to 12 nm The reading can be in Transmission Extinction or directly in Concentration values Gauging in units of concentration can be done by setting the factor or by use of standard gauge solutions by setting the concentration The number of decimals of the reading can be set separately The relation between the concentration and the light absorbency of the solution is described by Lambert Behr This laws only apply by use of monochromatic homogeneous light on one hand and on the other hand homogeneous liquid solutions without any effects such as refraction blocking reflection fluoridation etc lo Extinction log It lo Light intensity before the sample It Light intensity after the sample Extinction ExCxL from which 1 C EXL E Absorbency of a standard sample with a standard cuvet length by
4. USER MANUAL STARRCOL MANUAL 1 10 Master Registration Number 006 Rei _ mechatronics Manufacturer Mechatronics Manufacturing B V Phone 31 0 229 29 11 29 Fax 31 0 229 29 11 24 E Mail support mechatronics nl Website http www mechatronics nl P O Box 225 Postal address 1620 AE Hoorn The Netherlands De 13 Office address 1689 AN Zwaag The Netherlands Copyright Mechatronics BV All rights reserved Subject to changes without prior notice Issued by the After Sales Department of Mechatronics mechatronics Guarantee Guarantee The manufacturer guarantees to repair any manufacturing faults or defective materials or both in the products supplied Excluded are materials that become defective due to normal usage such as needles syringes glass ware tubing O rings etc The guarantee period commences on the day of delivery to the customer and shall be effective for a period of twelve 12 months No claims for guarantee can be made on Mechatronics Instruments BV if fluids and or spare parts of other manufacturers than those prescribed in the manual are used The manufacturer Mechatronics Instruments BV takes no responsibility for the consequences of errors which might appear in this manual in spite of every effort made in translations StaRRcol Manual Page 3 Version 1 10 mechatronics Contents CONTENTS 1 INTRODUCTION PPP o re ee 7 2 DIFFERENT MODELS
5. ace the desired cuvette adapter The adjust pin of the cuvette holder should fit into the groove of the measure chamber Fix the adapter with the locking screw on front of the instrument The StaRRcol can now be switched on StaRRcol Manual Page 15 Version 1 10 mechatronics Installation 5 1 StaRRcol Initialising procedure After switching on the display reads StaRRcol vrs xx After a few seconds the display reads REAL BLANKING During this time approx 15 sec the light source will be adjusted to most optimum dynamic range The light source will be adjusted in such a way an optimum is achieved in dynamic range and lifetime of the source After the REAL BLANKING procedure a stabilisation test is performed and the display reads STABILIZING br Every 20 seconds during this procedure will be checked if a minimum stability is achieved Only at a stability level better than 1 m Abs the stabilising procedure breaks automatically and starts absorbency readings The stability procedure can be interrupted by pressing the BREAK key The stabilisation time depends on the IFL filter and the condition of the light source The StaRRcol is ready for use now after it actually performed a blanking If time is important you can put your blank sample into cuvette before switching on the instrument In this way the instrument is stable and blanked in the most economically way of spending time StaRRcol starts the work
6. ing condition from its operation mode of latest use switching over to another measure method can simply be done by pressing the key FUNCTION and the key reading the desired method key 7 transmission 8 extinction 9 concentration StaRRcol Manual Page 17 Version 1 10 mechatronics StaRRcol design 6 STARRCOL DESIGN On the top side Figure 1 of the instrument are a keyboard a 16 character backlighted LCD display the measure unit located The keyboard has 16 keys the figures 0 9 and a few special keys and a shift key to activate special functions The top side of the instrument is made of a foil it covers the whole top side including the keyboard and the display Constructed this way the instrument is protected against liquids which might be spoiled over the instrument and it is also very easy to be cleaned The LCD Liquid Crystal Display has 16 characters sized 14 5 by 7 2 mm The measure unit is made of a plastic based rectangular block with a hole of 30mm diameter In this hole fits a holder which can hold several different shaped types of cuvettes Available are extractor cuvettes flow through cuvettes standard square circular cuvettes For huge cuvettes light path up to 60 mm special adapters are available On the front left hand side the light source is located behind a cover With an Allen key driver the light source can be adjusted to the most optimum performance highest
7. light of the concerning wavelength for example 450 nm E 330 mE 1 mmol sample 1 cm cuvette length C Sample concentration L Length of the light path through the cuvette 1 Factor E L From with follows factor E E and L are constants StaRRcol Manual Page 13 Version 1 10 mechatronics Measuring methods Out the above follows that the extinction is independent of the intensity of the light source The stability of the light source however is important for the calibration of the instrument with a sample standard With the sample blank there is no light absorbency assumed 50 o gt 1091 0 lo Concentration factor 0 0 The photometer measures the light intensity It To prevent the necessity to repeat the blanking calibration a stable light source and signal processing are of great importance The StaRRcol photometer is able to measure very accurate up to over 3 0 extinction The accuracy is up to 1 5 extinction better than 1 relative and up to 3 5 extinction 2 relative Page 14 StaRRcol Manual Version 1 10 mechatronics Installation 9 INSTALLATION Place the StaRRcol photometer on a flat stable table Shield the instrument from direct sunlight Check if the correct voltage has been selected and plug the cord in the instrument Connect if needed the printer computer cables Place the desired IFL filter in the filter unit at the front side of the instrument and pl
8. n the quick blank In practice the full blank will only be necessary by re placing another IFI filter The print key activates the print routine As well the value on the display is printed as the patient Id number It is also possible to activate the print command by an external signal remote connector Confirm key to enter accept values etc Escape key to break functions or key hits without changing former settings Page 21 mechatronics StaRRcol functions Page 22 StaRRcol Manual Version 1 10 mechatronics EEE StaRRcol functions 7 1 Routine measurements Place the desired IFL filter in the filter rack Place the desired cuvet holder and put in the blank sample Switch on the StaRRcol or press the BLANK key Set the desired mode after the blanking procedure When the transmission or absorption mode has been selected the StaRRcol is ready for use Replace the blank with the samples and read the display and or press the print key When the concentration mode is selected first the desired factor should be given in 7 1 1 Measurements by using a standard Place the blank sample and press the BLANK key Place next the standard gauge solution and press the FUNCTION key and the SET CONC key give in the concentration of the gauge solution and press ENTER The factor will be calculated and the display reads the keyed in concentration The calculated factor can be displayed by pressing the FUNCTION and the SET FACT
9. nector on the printed circuit board The jumper setting nearest to the connector has the highest baud rate 9600 The StaRRcol is provided with a battery RAM to save variables also when the instrument has been switched off StaRRcol Manual Page 35 Version 1 10 mechatronics Specification 9 2 StaRRcol Current Output 4 20 mAmp Optional 9 2 1 Dutch De StaRRcol kan als optie uitgerust worden met een stroomuitgang 4 20 mAmp Deze stroom loopt synchroon met de Extinctie waarde 0 4096 mExt en levert daarbij een stroom van 4 20 mAmp over een belastingsweerstand van maximaal 100 Ohm de belastingsweerstand mag liggen de range van 0 100 Ohm Als voorbeeld Bijeen Extinctie van 947 mExt levert de stroom uitgang een stroom van 4 947 4096 16 7 70 mAmp Bij een Ext Van 0 mExt levert de stroom uitgang een stroom van 4 mAmp De stroomuitgang is verbonden met pin 5 stroomuitgang de retouraansluiting is pin 6 7 8 9 ground van de 9 polige subD connector De stroomuitgang stelt zich automatisch in bij een blanking en levert dan per definitie 4 mAmp De lineairiteit is beter dan 1 relatief de offset is na temperatuur stabilisatie beter dan 4 relatief Belangrijk is dat het instrument eerst 20 minuten moet opwarmen voor de meest stabiele instelling 9 2 2 English This StaRRcol is equipped with a current output 4 20 mAmp The current output is synchronised with the Extinction outpu
10. output Behind the measure unit on the right hand side the filter unit is located The interference filter has a bandwidth of 8 and 12 nm The IFL filter is been placed behind the cuvet to minimise the effect of ghost light which shines from above into the measuring unit On the most right side of the instrument the spare filter rack is located The mains switch is located on the back side of the instrument just above mains cord Euro connector The parallel Centronics printer connector IBM compatible the remote control connector and the RS232 connector optional are located more or less in the middle of the rear panel We recommend that the connectors should be pushed pulled into the instrument only when the instrument is switched off StaRRcol Manual Page 19 Version 1 10 mechatronics StaRRcol functions 7 STARRCOL FUNCTIONS Transmission Absorption Concentration Full Scale Mode Blank Print Enter Break StaRRcol Manual Version 1 10 Use the function key in combination with key number 7 to activate the transmission mode The light which remains after it passed the sample is the transmission The transmission through the blank sample is by definition 100 Use the function key in combination with the number 8 key to activate the absorbency mode The display reads the Absorbency extinction light absorbed by the sample Use the function key in combination with the number 9 key to activa
11. parallel IBM compatible printer port The reliability is increased by absence of a fan mechanical chopper potentiometers etc Also the lifetime of the light source is optimised by the micro computer controlling the voltage and the current of the light source Through that the light source lights and heats the sample not more as strictly needed The Kohler optics sends through the cuvet and the filter an almost parallel homogeneous light beam In this way the best results can be expected StaRRcol Manual Page 7 Version 1 10 mechatronics TT Different models 2 DIFFERENT MODELS The StaRRcol photometer is available in two models Please contact your dealer for details e Standard including parallel printer port remote control and analogue output 0 4 V 0 000 4 000 Ext e As above mentioned including a RS232 serial port for a bi directional connection to a host PC computer system StaRRcol Manual Page 9 Version 1 10 mechatronics Sample measure unit 3 SAMPLE MEASURE UNIT The standard measuring unit is a cuvet holder that contains a continuous chamber diameter 30 mm In this chamber different sizes and types of measuring cuvettes with a length up to 25 mm can be placed The continuous open chamber prevents liquid flowing into the instrument The advantage of such a construction is that a continuous flow system can be implemented easily Special replacements are available for cuvettes light path length up to
12. path longer than 10 mm possible on request 16 Character 14 5 mm high 7 2 mm width 16 positions tinfoil with click IBM compatible 9 pins Sub D male connector Pin 1 6 4 shortened Pin2 RD Pin3 TD Pin5 Gnd Pin 7 RTS Pin8 CTS Pin 9 Centronics IBM compatible 25 pins Sub D female connector 9 pins Sub D female connector StaRRcol Manual Version 1 10 mechatronics Specification Remote print Analogue output Optional StaRRcol Manual Version 1 10 Pin 1 10 100 msec pulse TTL compatible high active Pin 6 7 8and 9 Gnd Pin 2 3 and 4 N C Pin 5 0 4 Volt Current output 4 20 mAmp Page 33 mechatronics Specification 9 1 RS232 protocol Connected the serial port of a PC StaRRcol can send data to a PC or when the connection is bi directional the StaRRcol can be controlled by a PC Three commands are available to set the StaRRcol in a particular mode Commando Mode 11 0B transmission mode 12 0C concentration mode 12 0D extinction mode The below standing command is having the StaRRcol transmitting a measurement result to the PC 01 transmitting a measurement StaRRcol sends totally sixteen ascii characters to the PC In fact the display reading is copied to the serial port The baud rate is intern adjustable to 1200 2400 default 4800 en 9600 baud The jumper to select the baud rate is situated opposite to the RS232 con
13. pears the display check the light source adjustment or replace it StaRRcol Manual Page 27 Version 1 10 mechatronics 71 IFL filter control 8 IFL FILTER CONTROL It is recommended to check IFL filter at least once a year It is very common to use a scanning spectre photometer in co operation with a chart recorder is very important to check not only the wave length but also the bandwidth and also to examine the double wave length StaRRcol Manual Page 29 Version 1 10 mechatronics Specification 9 SPECIFICATION Specification Weight IRG Dimension LxHxW StaRRcol Manual Page 31 Version 1 10 Specification Mains Switch over to Fuses Slow blow Dissipation Light source Photocell IFL filters Wave length range Dynamic range Accuracy light path Beam surface Cuvette holder Not standard holders Display Keyboard RS232 connection Printer connection Remote control Page 32 mechatronics 220 240V 50 60Hz 105 120V 50 60Hz 220V 0 5A 110 1 0A Slow Blow lt 35 W Wolfram 12V 15W Semiconductor Silicium 310 1050nm Several different wave length with a bandwidth of 8 12nm Wolfram 340 1000nm 0 4096 mAbs 300 3796 1mAbs Standard 10mm other light path on request max 50 mm 3x9 mm Circular 30 mm Several shapes are available as well as circular square sized drain cuvette flow cuvettes Cuvette holders for light
14. t The StaRRcol generates an output of 4 20 mAmp over the theoretically full range of 0 4096 mExt The maximum load resistant is 100 Ohm in other words should be equal or less than 100 Ohm Example An Extinction of 947 mExt generates a current output of 4 947 4096 16 7 70 mAmp An Extinction of O mExt gives a current output of 4 mAmp The output current flows from pin 5 and the returns at pin 6 7 8 9 ground of the 9 pin subD connector The current automatically zeroes 4 mAmp at blanking The current output linearity is better then 1 relative the offset is after temperature stabilisation better than 5 relative The instrument should warm up for at least 20 minutes to achieve the above mentioned figures StaRRcol Manual Page 37 Version 1 10 mechatronics Index 10 INDEX and display 25 Checking the light source 27 D DIFFERENT MODELS 9 Dutch 37 E English 37 G Guarantee 3 IFL FILTER CONTROL 29 INSTALLATION 15 INTRODUCTION 7 L Light source replacement 26 M Measurements by using a standard 23 Measurements with sample or reagents blank 23 MEASURING METHODS 13 R Routine measurements 23 RS232 protocol 35 S SAMPLE MEASURE UNIT 11 SPECIFICATION 31 StaRRcol Current Output 4 20 mAmp Optional 37 STARRCOL DESIGN 19 STARRCOL FUNCTIONS 21 StaRRcol Initialising procedure 17 StaRRcol Manual Page 39 Version 1 10
15. te the concentration mode The display reads the concentration This mode shows the concentration calculated out the absorbency multiplied by the factor The factor can be set by hand using the FUNCTION key combined with the number 6 SET FACT key Simply key in the factor including the desired decimals It is also possible to have the factor calculated put the standard solution with known concentration into the measuring unit press the SET CONC key and give in the concentration If done press the ENTER key the factor will now be calculated and saved By means of the FUNCTION key and the FULL SCALE key the decimals can be set or altered After the function is activated just type the desired decimals maximum 3 Mind that the accuracy of the StaRRcol always has to be taken into account Increasing the decimals will not increase the accuracy of the instrument By means of the FUNCTION key and the MODE key the user can give in the patient Id number This number together with the result will be printed after the print command This function activates the blanking procedure If after this command has activated the reading is between 50 and 300 mAbs a quick blank will be performed also called software blank If the reading exceeds the above mentioned values the instruments starts a full blanking procedure The light source is adjusted as described above The display reads REAL BLANKING The full blanking procedure takes more time tha
16. the accuracy of the instrument StaRRcol Manual Page 25 Version 1 10 mechatronics StaRRcol functions 7 2 1 Light source replacement On the left hand side front side a protecting shield is situated Unscrew the locking screw and remove the protecting shield The light source is locked with two allen keys turn the allen keys a few turns counter clock wise and remove the light source See also figure 3 Replace the light source with anew one and mount it again Do not touch the light source with your fingers or hand it will certainly shorten the lifetime of the light source Be sure the allen keys are firmed correctly and replace protecting shield Adjust the light source put the allen screw driver thus far into the two holes on the front of the instrument to minimum absorbency or maximum transmission If both screws are tightened or loosened equally the light source will move forwards or backwards If only one screw is tightened or loosened the light source will overturn a bit Page 26 StaRRcol Manual Version 1 10 mechatronics StaRRcol functions 7 2 2 Checking the light source Stability The stability of the light source is checked during the stabilising procedure during start up If this procedure takes longer than usual lt 120 sec check the light source adjustment or replace the source Light intensity The maximum light intensity is degreasing during its lifetime If during blanking the BLANK ERROR ap
Download Pdf Manuals
Related Search