OCS Quick Users Manual
Contents
1. 3 Auto exposure At first try e Exposure time 1 min e Binning Large or Medium e F Stop 1 Adjust time if needed 4 Acquire data IVIS Acquisition Control Panel Imaging Mode Exposure Time Binning F Stop Excitation Filter Emission Filter d Field of View system Status Status Idle Acquire N an Subject height 150 m Sequence Setup Sequence Setup Setup Focus use subject height eae Locked OOOO iiaee 5 Label you data file and save it in the Data temporary folder desktop Add an X Ray 1 Place your anesthetized mice in the chamber 2 Follow Bioluminescence Images procedure 3 Add a check mark next to X Ray F IYIS Acquisition Control Panel Imaging Mode Exposure Time Fstop Emission Filter Excitation Filter Binning Field of view Ic System Status X Rays will be produced de iitsi SCS when energized Service fio cm Acquire Subject height 0 00 cr amp Sequence Setup Focus fuse subject height Temperature ll Locked Initialize 4 Set up X Ray mode Lock the door handle completely Emergency switch in the ON out position Key is turned ON Push orange switch light must be ON The X ray source cannot be energized until these conditions have been fulfilled 5 Acquire data F IYIS Acquisition Control Panel Imaging Mode Exposure Time Binning Fstop Excitation Filter Emission Filter Field of2. view System Status Idle Acquire ie e me Subject height 150 Siem Sequence Setup 6 Label you data file and save it in the Data temporary folder desktop 7 Return the key to the OFF position Fluorescence Images 1 Place your anesthetized mice in the chamber 2 Turn on the Fluorescence light source lower shelf 3 Put a check mark next to Fluorescent I IS Acquisition Control Panel Imaging Mode Exposure Time Binning Fstop Excitation Filter Emission Filter eff E zforen puto H puto H Field of View v ot cm Subject height cm System Status Idle Acquire Sequence Setup 3 Select excitation and emission filters 4 Acquire data 5 Label you data file and save it in the Data temporary folder desktop When you are done 1 Take ALL the mice out of the IVIS chamber 2 Gently wipe clean all surfaces Use cleaning solution provided by DLAR Do not spray or pour cleaning solution into the chamber 3 Clean the anesthesia box Remove paper towels Wipe with cleaning solution What to do with the images 1 Close Living Image Software x in the upper right corner of the screen 2 DO NOT LEAVE YOUR IMAGES ON THIS COMPUTER Option 1 Export in your lab SAN space e Click on the Connect Research SAN icon desktop e Follow the prompts enter your SAN lab name XXXlab login with Kerberos ID and password drag your data onto your SAN space LOGOUT Click the Dis
3. OCS Small Animal Imaging Core Facility Quick Users Manual This is a self service facility It only works if everyone follows the procedures How to Start 1 Signup user logbook Login lViSuser Pwd user 2 Double click on Living Image Software icon _ System Status Aequirs Ide 12 8 cm PO subject height 1 50 cm Sequence setur This takes about 10 min You can prepare your animals during this time URL link to online manual http www caliperls com assets 022 8234 pdf Prepare your animals Only 3 mice at a time can fit together in the IVIS chamber 1 Inject animals with chromiluminescent substrate 2 Place a pad of paper towels inside the anesthesia box before introducing your mice 3 Anesthetize your mice Eae gt emy Paper towels here 4 Place your animal s in the IVIS chamber Plug unused stations Nose cone adaptor D o i y e Nose in the plastic nose cone adaptor e Make sure to plug the unused stations with rubber corks nose cones and plugs are in the table drawer 5 Take ALL the mice out of the IVIS chamber back into a cage asap Bioluminescence Images 1 Place your anesthetized mice in the chamber 2 Put a check mark next to Luminescent F IYIS Acquisition Control Panel Imaging Mode Exposure Time Binning Fstop Excitation Filter Emission Filter Field of View system Status O Status Idle Acquire ae Subject height Sequence Setup
4. connect Research SAN icon Option 2 Co our data on a USB flash drive e USB port on the left side of the monitor Option 3 Burn a CD 1 Delete your files from the computer 3 Logoff IViSuser 4 DO NOT Shutdown the computer 5 DO NOT turn off the main ON OFF switch in the back of the instrument Data Analysis Workstations Analysis software is available at 4 workstations e Ehrman Medical Library Graphic area e Smilow 308 e MSB 530 e HJD location TBD 2 letters lab user IDs elA Aifantis lab e AE Erlebacher lab e JH Hay lab e EH Hernando Lab e KS Kirsch lab e DL Levy lab e RN Novick lab e QO O Connor lab e HB Samuels lab oJI Torres lab e HB Samuels lab e YW Radiology lf your lab does not have a user ID contact Sheenah Mische Skirball 3 Please do not create your own lab ID PLEASE READ elf you don t know ask gt Luigi Scotto O Connor s lab ext 3 9123 Smilow 1306 gt Maria Guijarro Hernando s lab ext 3 9054 Smilow 3 e DO NOT TURN OFF the main ON OFF switch in the back of the instrument e DO NOT shutdown the computer e All data files left on the computer will quickly be erased e Leave the equipment clean e Leave the room in order
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