ÄKTApurifier 10 to ÄKTApurifier 100 Upgrade kit
Contents
1. Tools included in Tool kit 56 3008 20 included in existing system Fiber detachment tool 18 1111 16 included in existing system Connection diagram capillaries 56 1052 87 included in upgrade kit AkTApurifier User Manual box 18 1141 00 included in existing system or in the UNICORN CD AKTApurifier 10 to AKTApurifier 100 Upgrade kit 2 Instruction 56 3136 01 Edition AC Removing AKTApurifier 10 capillaries WARNING When using hazardous chemicals make sure that the entire system has been flushed thoroughly with bacteriostatic solution e g NaOH and distilled water before removing any capillaries WARNING Ensure that the mains supply is disconnected before starting the installation Remove all capillaries and connectors from the separation unit and the Pump P 903 The following items will be reused W1 W3 56 3047 57 Piston seal rinsing system 18 1113 32 Flow restrictor FR 904 18 1112 42 Inlet filters 18 1113 15 Removing KTApurifier 10 components Remove the 10 mm UV flow cell Use the fiber detachment tool 18 1111 16 Remove the four 10 ml pump heads including pistons and return springs Use the 3 mm Allen key included in Tool kit 56 3008 20 and save the attachment screws They will be re used AKTApurifier 10 to AKTApurifier 100 Upgrade kit 3 Instruction 56 3136 01 Edition AC Installing AKTApurifier 100 components ul Assemble the 100 ml pump heads according to instructions in the Pump P 900 User Manua2. Sweden imagination at work AKTApurifier AKTAdesign and UNICORN are trademarks of GE Healthcare Ltd a General Electric company GE imagination at work and GE monogram are trademarks of General Electric Company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2005 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric company GE Healthcare GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Instruction 56 3136 01 AC E Elanders sterv la 2006
3. B2 W1 W3 F3 and G9 to the two tubing holders on the rear side Instruction 56 3136 01 Edition AC Checking the installation When all of the installation is carried out check that all the connections and mountings are performed according to the diagram 56 1052 87 All excessive material is stored in Box 900 AKTApurifier 100 installation test Perform an installation test as described in the AKTApurifier Installation Guide When the test is finished disconnect G5 from the UV flow cell and connect the union 18 1120 93 to the cell instead AkTApurifier 100 test record Make a photocopy of the test record included in the AKTApurifier Installation Guide and store the test record in the AKTAdesign System Logbook Store also the packing lists in the System Logbook Accessories The accessories that are included in the upgrade kit is to complement the upgraded system to original delivery status Store the accessories in Box 900 or wherever convenient for the customer k This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment KTApurifier 10 to KTApurifier 100 Upgrade kit Instruction 56 3136 01 Edition AC www gehealthcare com GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala
4. inner part of the outlet manifold A pump heads and G2 to the outer part B pump heads Attach the on line filter 18 1112 44 to the G3 capillary and connect to the mixer outlet Observe the flow direction marked by an arrow Attach the other end of the on line filter 18 1112 44 to the G4 capillary and connect to V1 Injection Valve INV 907 Instruction 56 3136 01 Edition AC 10 Ti 12 15 14 15 16 17 KTApurifier 10 to KTApurifier 100 Upgrade kit Mount the sample loop 500 ul 18 1113 99 on V1 Connect W1 and W2 to V1 and put the open ends of W1 and W2 in a waste bottle Connect G5 to V1 and to the inlet of the UV flow cell top connection Connect G6 from the outlet of the UV flow cell bottom connection to the conductivity flow cell The flow direction of the conductivity flow cell should be so that the screw head end of the flow cell faces the flow restrictor Attach the flow restrictor FR 904 marked IN to the G7 capillary and connect to the conductivity flow cell The flow restrictor should be housed in the hole in the pH flow cell holder Attach the other end of the flow restrictor to G8 and connect to V4 outlet valve PV 908 Connect W3 Put the open end of W3 in a waste bottle Connect G9 If using a fraction collector refer to its user manual for information on how to connect the tubing to it Connect F3 Put the open end of F3 in a flowthrough container Attach A1 A2 B1
5. GE Healthcare AkKTApurifier 10 to AKTApurifier 100 Upgrade kit Instruction Conversion kit code number 18 1148 56 IMPORTANT Read through this instruction in whole before commencing with the installation Contents SEINE EY EEE EEE AEAEE E AEE EE EEE E EE E EEEE EE EEEE E AEEA EEA EEE A 1 UNPACKING aeren E E EEEE E E EEN E EAEE E censvovivantessssevenen ee 2 SOFTWARE PRE REQUISITES iecticsi tee ccescets stots aden ia eiras aia eeri ar aia aene s Aee Ea ra Aora iaeaea aeeie tat aresta aaae inara Naia 2 REQUIRED TOOLS AND DOCUMENTS sressnyneriaran hreini aaan RA AR iA EN ARAARA ERR 2 REMOVING AKTAPURIFIER 10 CAPILLARIES assscccccssssseessssssssssssssssssesceccesscesseususssssssvvssssssssssssssssssssseseeseesecesceussstusiiniesssssssssssssssssssesssssseeeeeseseee 3 REMOVING AKTAPURIFIER LO COMPONENTS vceccecccscsssesssssssssssssssssseecesceeseesscsessesssssesssssssssssssssssssssseseeeeeesececcesssiuitiuisesssssssssssssssssssesssseseeeeeeessee 3 INSTALLING AKTAPURIFIER LOO COMPONENTS veeseeccsssssssssssssssssssssescescseseesscsssstssssssessesssssssssssssssssseeeeeeeesececestsssuisitiensesssssssssssssssssesesseseeeeeseeeee 4 INSTALLING AKTAPURIFIER LOO CAPILLARIES ecccccscsssssscssssssssssssssescssceescesecetssssssssssssssssssssssssssssssseseeeeesecesseesssiuisimuesesssssssssssssssssssssseseeeeeeeeesee 4 CHECKING THE INSTALLATION nsir e sees eie ENEE E ATTE ETEEN 6 AKTAPURIFIER LOO INSTALLATION TEST ccssssssssscsssscssssssssssccscccsscessssssss
6. l Changing piston sealings Mount the 100 ml pump heads Please observe the UP marking Fit in position and fasten using the old attachment screws Mount the piston seal rinsing system capillaries Please observe the flow direction of the check valve arrow should point towards the front of P 900 Mount the pump head capillaries The capillaries from the A pump heads should be connected to the inner part of the outlet manifold Mount the inlet manifolds on each pump module and fix with the screwed end pieces do not overtighten Attach the Switch valves SV 903 to the mounting rail on P 900 Check that the control cable is running free Mount the 2 mm UV flow cell 18 1111 10 Use the fiber detachment tool 18 1111 16 Slide the rubber sleeves on the two optical fibers onto the connectors Make sure that the sleeves are pushed tight to the housing to prevent dust or fluid from entering the connections Note Fit all the protective caps on the old 10 mm flow cell Installing AKTApurifier 100 capillaries 1 KTApurifier 10 to KTApurifier 100 Upgrade kit If not already done unpack and check the KTApurifier 100 tubing kit cpl 18 1112 78 Change the inlet filter inserts 18 1114 42 if required Attach the inlet filters to capillaries A1 A2 B1 and B2 Connect the inlet capillaries A1 A3 and B1 B3 in that order according to diagram 56 1052 87 Connect G1 and G2 to the 2 ml mixer chamber inlets G1 is connected to the
7. ssssssssssssssssssssssstsissssseseeceseesesessssssssssssssssssssesessstsissssessesesssesssssssssssssssssees 6 AKTAPURIFIER 100 TEST RECORD ekreis aia iasa idast ataare assia sia atia Esai Er E Ninia ARENAN 6 ACCESSORIE hunn aE A A NE R E E EN ERE 6 General The KTApurifier 10 to KTApurifier 100 upgrade kit converts the flow rate from 10 ml to 100 ml and maximum pressure range from 25 MPa to 10 MPa P903 is converted to P 901 The kit also includes capillaries and other components in the flowpath Unpacking Unpack the AKTApurifier 10 to AKTApurifier 100 conversion kit and check the items against the supplied packing list Inspect the items for obvious damage which may have occurred during transportation Please note that some of the included packages have their own packing lists included Check these as you go along It is recommended to reuse the packing material to pack the surplus AKTApurifier 10 equipment for possible future use Software pre requisites Before you start disassembling the AKTApurifier 10 system install UNICORN version 3 21 or later Then install the AKTApurifier 100 strategy from the same CD General installation instructions for UNICORN software are given in the UNICORN User Manual IMPORTANT Make sure that the existing AKTApurifier 10 system is equipped with purge valves on the pump heads If not contact the Technical Support Department of GE Healthcare Bio Sciences AB Required tools and documents
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