Perfusion Valve Controller User`s Manual
Contents
1. Instruments Operating Directions Before beginning setup take an inventory of the plastic fittings supplied with the complete system These fittings include eight 8 LPE 50 tube to Luer connectors and eight 8 stopcock with Luer connectors Connectors are shown below 7 N gt Y 2 gh LPE 50 Luer to PE Stopcock with Luer MLS manifold tubing connector connector Setup The diagram below shows connections for the VC 8M Perfusion System The valve manifold comes with plugs to block flow to the NORMALLY OPEN flow lines Remove these plugs and use these ports if a flow to waste system is desired In general the shortest response time of the system will be achieved by keeping the tubing length between the MANIFOLD and sample as short as possible _ Syringe Stopcock n IM Luer to PE tubing connector Valve Manifold fos Nomally Closed Port Common Port Nomally Open Port Mini Valves PE 50 tubing X Manifold Publication 5714 001 REV A 2 Perfusion Valve Controller Model VC 8M Warner Instruments Operating Directions conta The VC 8M Valve Controller is assembled in the following manner First attach the VALVE MANIFOLD to the RING STAND as shown below This is followed by insertion of PE 50 tubing into the valve ports on the VALVE MANIFOLD Depending on the flow arrangement you re using on off or flow to waste cut2. Perfusion Valve Controller Model VC SM WARNER A INSTRUMENTS A Harvard Apparatus Company CE Publication 5714 001 REV A WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any ch
3. Replace fuse according to voltage being used For 120 VAC 0 50 Amp 5 x 20 mm Slow Blow For 220 VAC 0 25 Amp 5 x 20 mm Slow Blow Instructions for Use Flow Adjustment Flow rates can be adjusted by raising or lowering the reservoir holder as well as by adjusting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the spec ified height with the supplied Teflon tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min mon 2n 20m Flow rates as a function of reservoir height Publication 5714 001 REV A 5 Perfusion Valve Controller Model VC 8M Warner Instruments Maintenance Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to man ufacture the system If salt solution spills on the valve assembly it should be cleaned as soon as possible with a soft cloth dampened with a mild solu tion of detergent and water NOTE Teflon and Lee Mini Valves must be completely flushed with distilled water after each use Permanent damage will result if saline solution is allowed to crystallize inside the valve The exterior of the CONTROLLER may be cleaned periodically to remove dust grease and other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Avoid ab
4. TL inputs is selected by placing the 2 position COMMAND INPUT TOG GLE into external digital mode Use of these inputs can allow the opening of more than one valve at the same time from a digital source such as your computer Placing the ON OFF TOGGLE SWITCH into the on position places the asso ciated valve into its open state A lit LED indicates the open state of the valve This setting is overridden by the overflow sensor Placing the ON OFF TOGGLE SWITCH into the off position places the valve into its closed state An unlit LED indicates the closed state of the valve Placing the ON OFF TOGGLE SWITCH into the ext position activates the associated BNC inputs A logic level low 0 V applied to the BNC places the associated valve into the closed state Correspondingly a logic level high G to 5 V places the associated valve into the open state A lit and an unlit LED indicates the open and closed states of the valve respectively These settings can be overridden by the overflow sensor Command Input Toggle The COMMAND INPUT TOGGLE is used to select between the front panel external digital controls and the rear panel external analog controls Publication 5714 001 REV A Perfusion Valve Controller Model VC 8M Warner Instruments Control Description Conto Overflow The OVERFLOW LED indicates an overflow condition sensed by the OVER FLOW SENSOR Activation of this circuit by an overflow condition immedi ately overrides all oth
5. anges as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 2 Perfusion Valve Controller Model VC 8M Warner Instruments Table of Contents IntrodU clinic 2 ON 2 Operating DIrectionSs srsesrrreserseseesesee ce see ce ceenenee 3 SPP 3 SO o 5 Control DescriptiON ccacacocinnmmrs 6 Front O PA 6 FIL Inputs 30 is A A ghaa 6 Command Input Toggle mimsinaanaanannaanannannannannannenni 6 OVETIOW o ed hh AA 7 PIO all FANON aia 7 D iGonneciora scans Sethe An A 7 Over OW IN karmis A Gela 7 A alan lalla iii 7 EXLANAIOG Mts ont bai O Sanne a AE 8 O a a a EER 8 Power Input Module scserssiiirisioniinanianiaaa 8 AG CONVERSION a S ae enaa T 9 Instructions for Usa das 9 Flow AdjUsIM Nt SS A 9 Male ria aaataa a aaaiaaa 10 Clea cinneann iae a e a 10 A O E 11 APP 12 OPECHICAlONS ada ili 12 Declaration of Conformity rmraunuauenenunnnnunnnnnnnens 13 Publication 5714 001 REV A Perfusion Valve Controller Model VC 8M Warner Instruments Introduction The VC 8M Valve Control System lies at the heart of a multi valve perfusion system designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers However its flexible design allows the system to be used in many applications not using Warner equipment The complete system includes the valve controller a
6. either eight 8 or sixteen 16 pieces of PE 50 tubing long enough to reach from the VALVE MANIFOLD to the PERFU SION MANIFOLD and from VALVE MANIFOLD to the your waste container Attach cut tubing to the output ports on each MINI VALVE on the VALVE MANIFOLD by gently pressing into place Port positions are shown in the figure on page 4 Place flow to waste tubing into the waste container and attach sample tubing to the PERFUSION MANIFOLD syringes syringe holder PE 50 tubing valve manifold ring stand valve controller Next attach the SYRINGE HOLDER to the RING STAND Affix a LUER TO PE 50 TUBING connector to each STOPCOCK WITH LUER connector Mount SYRINGES into the SYRINGE HOLDER and attach a prepared stop cock assembly to each SYRINGE Now cut six 8 pieces of PE 50 tubing just long enough to run from the LUER TO PE 50 TUBING connector on the SYRINGE to the input port on each MINI VALVE Finally make a connection between the perfusion MANIFOLD and your sample with PE 50 tubing Plug the VALVE MANIFOLD into the VALVE CON TROLLER and the system is ready to use Publication 5714 001 REV A s Perfusion Valve Controller Model VC 8M Warner Instruments Operating Directions conta Overflow Sensor An overflow or spill sensor is has been provided to assist you in providing a level of protection to your microscope while using the VC 8M Valve Control System The overflow sensor itself is a Ag AgCl probe which ground
7. en valve and open the other Valve selec tions are overridden by the OVERFLOW SENSOR and the STANDBY BNC The table below indicates the selection scheme Applied Voltage V Valve Selection standby foe ee Event Out The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned on At all other times the Event Marker output is low 0 V Power Input Module A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with a NEMA 5 15P connector is standard A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 5 x 20 mm fuses of the type indicated Publication 5714 001 REV A s Perfusion Valve Controller Model VC 8M Warner Instruments Control Description Conto AC Conversion ATTENTION PLEASE READ BEFORE APPLYING POWER TO YOUR UNIT The unit uses only one fuse the second one is sent as spare Follow these instructions to change the unit s from 120 VAC or 220 VAC Power Entry Module Fuse holder side view Push out drawer to access the Spare Fuse l Active Fuse i A Carefully pry open the fuse holder from the inside Depending on AC Voltage being by using a small flat screwdriver used turn VAC selector switch A to 110 VAC or 220 VAC
8. er settings and closes all valves Rear Panel The rear panel contains the power input module with fuse a 15 pin D con nector for the VALVE SET and 4 BNC connections for OVERFLOW IN STANDBY IN EXTERNAL ANALOG IN and EVENT OUT Y CAUTION S a NOT FOR CLINICAL WARNER USE ON HUMANS INSTKUMENTS 115 230 60 50 Hz 0 5 0 25A Slow 8low OVERFLOW STANDBY EXT ANALOG EVENT IN N N OUT D 20 pa s O Q MADE INUSA D Connector A 15 pin D type female connector is used to connect the VALVE BRACK ET and valves to the CONTROLLER Overflow In This BNC is used to connect the OVERFLOW SENSOR to the controller Place the sensor into position and plug it into this BNC Standby In This BNC is provided to allow the placing of the VALVE CONTROLLER into standby mode Standby mode disables EXT ANALOG IN inputs and closes all valves Activation of this input is selected by placing the FRONT PANEL COM MAND input toggle into external analog mode and a TTL level high G to 5 V places the instrument in standby Publication 5714 001 REV A gt gt o o o D 2 CL o o D iz Gi gt s 2 wn pa lt D a A std il TAS Warner Instruments Control Description Conto Ext Analog In This BNC is used to operate valves using analog voltage settings The volt age applied to this input selects the valve to open All other vales are closed Switching voltages closes the op
9. nection to OVERFLOW SENSOR Triggered when sensed resistance falls below 10 MQ Standby In BNC connection to activate standby mode TTL high closes all valves Ext Analog In In BNC connection for valve selection Active valve opened by analog voltage Event Marker Logic level pulse 500 ms nominal rear panel BNC connector Manifold 2 1 4 1 or 6 1 nominal dead space Power 110 to 130 or 200 to 250 VAC single phase 50 60 Hz 20 W Operating Temperature 10 to 40 C 50 to 104 F Dimensions H x W x D 89 x 203 x 305 mm 3 5 x 8 0 x 12 in Weight Shipping Weight 3 7 kg 8 lb 4 6 kg 10 Ib Operating Conditions Equipment is intended to be operated in a controlled laboratory environment Temperature 0 to 40 C Altitude Sea level to 2000 m Relative Humidity 0 to 95 Publication 5714 001 REV A Declaration of Conformity CE MARKING LVD amp EMC Application of Council Directive 73 23 EEC 89 336 EEC Standard s to which conformity is declared EN61010 1 1993 EN35022 Class A EN61000 3 2 EN61000 3 3 EN30082 1 1992 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Manufacturer s Name Warner Instruments LLC Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 T 203 776 0664 Equipment Description Valve Controller Equipment Class Class I ITE Class A Model Numbers VC 8 Controller I the undersigned hereby declare that the equipment specified above conforms to the above Directive
10. rasive cleaners Publication 5714 001 REV A 5 Perfusion Valve Controller Model VC 8M Warner Instruments Warranty The VC 8M is warranted to be free from defects in materials and work manship for a period of two years from the date of shipment If a failure occurs within this period we will repair or replace the faulty component s at our discretion This warranty does not cover failure or damage caused by physical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Publication 5714 001 REV A 3 Perfusion Valve Controller Model VC 8M Warner Instruments Appendix Valves 12 VDC 0 46 A solenoid type Tubing PE 50 Valve Bracket Teflon Mounts on 3 8 or 1 2 ring stand Connection Cable 2 4 meter 8 ft connecting cable terminated with quick disconnects on valve end and 15 pin male D type connector on controller end Reservoirs 10 cc capacity syringes Reservoir Holder Delrin Holds eight syringes with thumb screws for each reservoir Valve Controller front panel Power Two position on off with power on LED Input Select Two position selecting analog or digital inputs Switch Selection On manual Off or External External Input 5 V TTL compatible BNC Connector Overflow LED indicating overflow condition Valve Controller rear panel Overflow In BNC con
11. s and Standard s Place Hamden Connecticut U S A Signature Ja yl M Full Name Ralph Abate WARNER INSTRUMENTS A Harvard Apparatus Company Position Business Manager
12. s through the chamber bath A resistance of 10 MO or less between the probe and bath will trigger the overflow circuitry Place the probe adjacent your perfusion chamber such that the maximum solution height say just prior to an actual overflow is achieved when the solution contacts the tip of the probe Contact between the probe and your solution will activate the overflow circuitry and close all the valves The OVERFLOW SENSOR plugs into the OVERFLOW IN BNC on the rear of the controller Publication 5714 001 REV A 2 Perfusion Valve Controller Model VC 8M Warner Instruments Control Description Front Panel The front panel contains TTL inputs for each valve a 3 position toggle switch for manually setting the state of each valve and an LED displaying the active status for each valve There is also a 2 position toggle switch for selecting the command input mode a SPILL SENSOR indicator and a power switch with power on LED a A x POWER 1 2 3 4 5 7 8 ayek FLOW i j j 5 j 3 gt EXTERNAL ON ON ON ON ON ON ON ON ANALOG 3 or Gor A or 3 or or or Mor Horo Y EXTERNA EXT EXT EXT EXT EXT EXT EXT EXT DIGITAL INPUT 1 INPUT2 INPUTS INPUTS INPUTS INPUT amp INPUT INPUTS 3 WARNER DIGITALINPUTS C 8 VALVE CONTROLLER INSTRUMENTS TTL Inputs TTL inputs front panel BNC s and ON OFF TOGGLE SWITCHES are pro vided for all 8 channels of the VC 8 controller Operation of the front panel T
13. valve bracket with mini valves connecting cable PE 50 tubing an ML Series manifold a ring stand eight 10 cc syringes and an assortment of tubing connectors The miniature solenoid valve assembly is standard with the system They are lightweight and made from rugged materials for long life Valves are dual act ing with both NORMALLY OPEN and NORMALLY CLOSED sides The NOR MALLY OPEN side is plugged with removable stoppers and when these are removed solution can flow to waste while the valve is in the off position A new and exciting feature to the VC 8 system is the Spill Sensor This probe is used to detect the presence of an overflow condition and when activated it will automatically shut off all valves protecting your microscope optics The controller can independently regulate the function of up to eight valves Individual valves can be controlled via a manual switch an external analog signal or an external digital TTL signal An event marker pulse gen erated each time a valve is switched on is provided at the rear of the instru ment for recording into your acquisition system Valve transitions opened or closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Features Mini valve system e 8 channels Spill sensor e Manual and computer control Publication 5714 001 REV A s Perfusion Valve Controller Model VC 8M Warner
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