aversive stimulation with video monitoring
Contents
1. Box Subject Experim Group Load Program 1 2 3 15 6 EN 1 14 18 19 A 23 E il 0 0 0 1315 AvStimVideo Issue pulse to EXIT program 0 00 Step Duration sec 1 25 Stepy amp 250 5 0 00 Max Current p 1000 00 SHOW S B 10 Acclimation sec 5 00 SHOWS 11 15 Inputs OK 0 00 SHOWS 16_20 SHOWS 21_25 SHOWS 26 30 4 10 2007 1 16 10 14 The AvStimVideo procedure displays the following data in the Show fields refer to Figure 2 Show 1 Show 2 Show 3 Show 4 Show 5 Show 6 Show 11 Show 12 Show 14 Issue K pulse to EXIT program instructions for terminating experiment before maximum current is reached Step Duration sec time spent at each current step after the acclimation period Step pA amount of current that the aversive stimulus will be increased by with each step Shock pA the amount of current the ENV 413 is set to transmit After the Start Command is issued this value will indicate the procedure s progression along the Aversive Stimulus ramp up model Max Current pA the maximum current output before the procedure is terminated Acclimation sec the duration of time the acclimation period The current will begin ramping up at the end of this2. Notice that Shows one through ten are blank and 11 through 15 indicate an error with the user input Comments field Show 11 USER INPUT ERROR two quoted strings were not found in the Comments field Show 12 Please put computer and camera part one of the error message Show 13 name in COMMENTS field part two of the error message Show 14 usage Computer Camera proper usage of the Comments field to indicate the Video Monitor Computer and Camera Show 15 e g Cam1 example of proper Comments field usage indicating that Video Monitor is running on the same computer as MED PC and the camera name is Cam1 16 4 5 Running the Experiment After the AvStimVideo procedure has been loaded by MED PC IV and Video Monitor has a video window titled Camera SAC 1 Waiting for remote trigger the experiment is ready to start Now is a good time to load the test animals into the boxes Issue Start Command After the animals are loaded the SACs are closed and the video streams are checked for image quality you are ready to start the test procedure Pull down the MED PC Configure menu and choose the Signals option The Send Signals to Boxes dialog will appear Figure 14 Figure 14 MED PC Send Signals to Boxes Dialog Send Signals to Boxes x Command to Issue Issue START Command Issue Issue K Pulse x Synchronize Occurence Close Hel Simulate Response z
3. meme E 9 MED PC Configuration eiue data ee Dis 11 MED PC Display unti el iE ER 14 Chapter 5 17 Running the Experiment em een stares 17 SSUG Start COMMAN MA 17 Stopping Experiment Before Reaching Maximum Current 1 22 17 Chapter 6 19 Video Playback and Arialysls irr et tpe RE Ee veda etat 19 Chapter 7 20 Using Open Session Dialog Instead of Wizard 20 Chapter 22 Using Macro uiii ce milan beh eee peeled ae el 22 Chapter 9 Troubleshooting No Session Parameters USER INPUT ERROR Message 1 Introduction Overview Aversive Stimulation with Video Monitoring allows the user the ability to use MED PC Video Monitor and Aversive Stimulation testing equipment to record aversive reaction in lab animals Background Researchers at a large university wanted to determine the average minimum current necessary to cause an aversive reaction in mice These researchers used an aversive stimulation cage connected to a MED Associates ENV 414
4. Table 1 Installation order Two Computer System Single Computer System MED PC I V Computer Video Monitor Computer 1 Install MED PC IV 1 Install MED PC IV 4 Install Video Monitor 2 Install Video Monitor 2 Install Video Monitor MED PC Interface 3 Install AvStim 3 Install AvStim Single Computer System Installation Procedure The single computer system is the recommended configuration to avoid network communication difficulties The installation order is as shown in the leftmost column of Table 1 above Note steps 2 and 3 may be performed in reverse order 1 2 Install MED PC IV Refer to the MED PC IV User s Manual for details Install Video Monitor Refer to the Video Monitor User s Manual for details In addition to installing the Video Monitor program this installation procedure will install the Video Monitor MED PC Interface components into the MED PC IV folder The installation procedure will search for the MED PC IV installation directory and automatically install the interface components there If the installation program is unable to find MED PC IV the Interface components will not be installed The Video Monitor MED PC Interface component installation will put the MED VM Interface DLL and VM Iface HED files in the MED PC IV installation directory the VI DEO MPC file in the MED PC IVAMPC folder and insert 1 VM IFACE HED into the User PAS file Install Aversive Stimulation with Video Monito
5. Figure 9 After loading any additional boxes select 1 Am Finished with the Wizard to close the Wizard and show MED PC MED PC Display After closing the Wizard by choosing Am Finished with the Wizard the MED PC application will appear as shown in Figure 11 Figure 11 MED PC Application Showing AvStimVideo Loaded in Box 1 MED PC IV B x File Configure View Macros Help aloje as EIKI Box Subject Experim Group Load Program 1 0 0 0 NENNEN 1315 AvStimVideo Ed Box 1 Step Duration sec 1 25 Stepy amp 250 5 0 00 Current pA 1000 00 Box 2 Issue pulse to EXIT program 0 00 4 10 2007 1 16 01 PM The MED PC display area is broken into 3 main sections Status Display Output Display and Show Area In Figure 11 the Status Display area at the upper left shows that Box 1 has the AvStimVideo procedure loaded The left most column header of the Show Area indicates that the shows are from All Boxes and shows one through five can be seen for each box Click the Boxes column header to cycle through the boxes The first click will alter the Show Display to present all the Shows for Box 1 See Figure 12 Figure 12 MED PC Application Displaying Box 1 s Show Statements MED PC IV pi x File Configure View Macros Help 41 a EA
6. MED PC needs to be told the name of the Video Monitor computer If MED PC and Video Monitor are running on the same computer the computer name is In a two computer system to find the Video Monitor computer name 1 Open to the Start menu 2 Right click on My Computer 3 Choose Properties 4 Click the Computer Name tab The computer name is the text before the first period in the Full computer name field See example in Figure 6 The computer name here is testxp Leave off the domain information car inavt us when specifying this computer in the MED PC session comments field Figure 6 Finding Computer Name testxp System Properties System Restore _ Automatic Updates Remote General Computer Name Hardware Advanced Windows uses the following information to identify your computer on the network Computer description TestMachinexP For example Kitchen Computer or Mary s Computer Full computer name testxp car inavt us Domain car inavt us To use the Network Identification Wizard to join a Network ID domain and create a local user account click Network To rename this computer or join a domain click Change Cancel Apply MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING Setup Camera in System Settings Dialog Figure 7 Video Monitor System Settings Dialog System Settings PCI bus 6 device 3 Function 0 onjoff Cameras On Current Card 1 Cameras On C
7. Now while playing the video a researcher can identify visual cues indicating stimulus response Notice in Figure 15 that annotation 7 Stimulus Response was added to the Default behavior set by the researcher in preparation for noting reactions to the aversive stimulus 19 MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING CHAPTER 7 Using Open Session Dialog Instead of Wizard If the Wizard is not used for loading the AvStimVideo procedure the Open Session dialog can be used instead To use the Open Session dialog pull down MED PC s File menu and choose Open Session See Figure 16 below Figure 16 MED PC Open Session Dialog Open Experimental Session x Subjegt Experiment SS Automatic Filename Group 000 C Custom Filename Procedure AVSTIMVIDEO Session Comment SACI Boxes to Load OK 1 rea Close Select All Deselect All Help Enter the Video Monitor computer name and the camera name in the Session Comment field The AvStimVideo procedure will NOT WORK without these parameters encased in double quotes The example shown in Figure 16 shows Video Monitor is running on the same computer as MED PC and the camera name is SAC 1 To alter the five input variables controlling step height step duration acclimation current acclimation period and maximum current use the Change Named Variables Dialog Figure 17 Pull down the Configure menu and choose the Change Va
8. SHOWS 51 55 4 4 2007 2 59 59 PM If the AvStimVideo procedure cannot find two quoted strings in the Comments field error message is displayed in MED PC s Shows area The first quoted string represents the Video Monitor computer name and the second quoted text is the name of the camera in the box named in the Shows area left most column header In Figure 20 the left most column header is Box 1 To fix this problem start the Wizard or the Open Session dialog and enter the computer name and camera name in the Comments field For example if loading AvStimVideo into Box 1 enter SAC 1 where indicates Video Monitor is running on the same computer as MED PC and SAC 1 is the name Video Monitor calls the camera in Box 1 24
9. 1 25 seconds e Maximum Current default and maximum 1000 0 uA The AvStimVideo MPC program allows early termination of the stimulation model before the maximum current is reached by issuing a K pulse This will be explained in greater detail in the Stopping Experiment Before Reaching Maximum Current section below Video Monitor Communication The AvStimVideo MPC program Opens communication with Video Monitor using the MED Video Monitor Interface Sends a Start Camera command to a camera waiting in Video Monitor The command travels via the communication pipe to begin saving a video file WMV or MEV Sends annotation messages at each aversive stimulation current step to appear as captions on the saved video Sends a Stop Camera command at end of Aversive Stimulation program or when terminated early by user input to end the video file saving Closes the MED Video Monitor interface communication channel Aversive Stimulation with Video Monitoring Components AvStimVideo is comprised of 4 software files AvStimVideo MPC MedState Notation code compiled using Transl V and run using MED PC The code controls the ENV 413 Aversive Stimulator and communicates with Video Monitor AvStim spt HED BOD Delphi Pascal code used by AvStimVideo MPC to parse the computer and camera name from the MED PC session comment field AvStimSample MAC MED PC macro code to simplify loading AvStimVideo entering c
10. __H Boxes T 2 Select All Deselect Al Choose the Issue START Command radio button and select the Boxes that are running the experiment Press the Issue button to start the Aversive Stimulation procedure Stopping Experiment Before Reaching Maximum Current The AvStimVideo MPC code will increase the current at a rate determined by the Step Duration and Step Increment values specified in the Wizard s Session Parameters screen The procedure will stop the current to the grid floor when the Maximum Current is reached and the video saving will be stopped To stop the experiment before the maximum current is achieved issue pulse to the box es Pull down the Configure menu and choose the Signals option Select the Issue K Pulse option set the number of K pulses to 1 and choose the boxes you wish to stop Press the Issue button to send the K Pulse The shock current will be turned off and the video file will stop saving NOTE If the AvStimVideo procedure is stopped without first issuing K pulse the shock will continue for up to one minute Also the video file will continue recording So do not end the procedure early by using the Close Session command or shut down MED PC without first sending a K pulse to the box running the procedure If this does happen go to Video Monitor and close the video window to stop recording To stop the shock unplug the ENV 412 to ENV 413 connection or turn off the p
11. time The initial base Shock pA current will flow through the grid floor during this acclimation period Inputs OK indicates that there were two quoted strings found in the Comments field The strings represent the Video Monitor computer s name and the box s camera s name Camera Started Camera Stopped after the Start command this field will indicate the camera has started After a K pulse or maximum current has been exceeded this field will indicate the camera was stopped Time sec duration of ramp up execution not including acclimation period The above Show statements indicate that the AvStimVideo procedure is properly configured If the procedure was not properly configured the Show statements will look like those in Figure 13 MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING Figure 13 MED PC Display indicating missing Comments parameters MED PC IV l File Configure View Macros Help 41 s 8 sm e Box Subject Experm Group 4 Proqram 1 2 EN 4 5 6 7 8 ERR hizhshafishish 18 21 22 23 24 25 26 27 28 29 30 3 SHOWS 6_10 SHOWS 11_15 INPUT ERROR 0 00 Please put computer and camera 0 00 name in COMMENTS field 0 00 usage Computer Camera 0 00 e a Cami 0 00 SHOWS 16 20 SHOWS 21 25 SHOWS 26 30 SHOW S 31 35 SHOWS 36 40 SHOW S 41 45 SHOWS 46 50 SHOWS 51 55 x 4 4 2007 2 59 59
12. AC 1 Waiting for remote trigger Adjust the camera features settings now to show a bright clear image MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING MED PC Configuration Translate and Compile AvStimVideo MPC Before compiling AvStimVideo MPC close any running MED PC programs From the Windows Start menu or desktop start Trans IV Pull down the Translation menu and choose Translate and compile The Translate and Compile dialog will appear see Figure 8 Highlight the AvStimVideo MPC file and press the Make button Next press the OK button Figure 8 TranslV Translate amp Compile dialog I Specify Files to Translate 926FREQ MPC Make Copy of ShockRamp MPC Build fr5wmpc mpc MacroTest MPC ShockRamp MPC i 1 fps MPC 100fps MPC 10fps MPC 20fps MPC MPC MPC MPC No Trans Exclude P4 ed et PE PLE v OK VMConmunicate MPC X Cancel Run MED PC Wizard Start MED PC If the Wizard does not automatically appear go to the File menu and select Wizard for Loading Boxes Press the Next button to continue to the Box Selection dialog Choose the first box that will be running the AvStimVideo program and press the Next button Load AvStimVideo MPC At the Select a Procedure screen highlight the AVSTI MVI DEO procedure and press the Next button The Experiment Info dialog seen in Figure 9 will appear MED ASSOCIATES INC AVER
13. AMERSI VE STI MULATI ON wi TH VI DEO MONITORING USERS MANUAL SOF 732 4 User s Manual DOC 155 Rev 1 0 Copyright O 2007 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING TABLE OF CONTENTS Chapter 1 1 MET EE 1 A qur 1 Backgrourid una dtd 1 a 2 En 2 Chapter 2 3 Aversive Stimulation with Video Monitoring Software 3 Aversive Stimulation Control 3 Video Monitor Communication 4 Aversive Stimulation with Video Monitoring Components 4 Chapter 3 5 Software Installation 5 Single Computer System Installation 6 Two Computer System Installation 7 Chapter 9 Setting Up Aversive Stimulation with Video Monitoring 9 Video Monitor Configuration
14. EXPT 0 GROUP 0 PROGRAM AvStimVideo COMMENT BOX 1 ComputerName CameraName START BOXES 1 Reading line by line the macro file l Loads the AvStimVideo procedure into box 1 2 Creates Session Comment for box 1 ComputerName CameraName 3 Starts the AvStimVideo procedure in box 1 Using this macro file as a starting point change ComputerName to be the name of the Video Monitor Computer and CameraName to the name Video Monitor uses to define the camera in box 1 The computer name and camera name must each surrounded by double quotes x Save the file as a unique name in the C MED PC IV Macro folder Note the name of the MED PC IV installation folder may be different on the MED PC computer To use the macro file configure Video Monitor so the camera is waiting for the start trigger from MED PC then start MED PC and cancel the Wizard if it appears MED PC pull down the Macros menu and choose Play Macro Select the file saved in the C MED PC IV Macro folder and click OK Using the macro file the AvStimVideo procedure will use the default values for the five input parameters If values other than the defaults are desired remove line 3 from the macro START BOXES 1 Save and run the macro Now use the Change Named Variables Dialog Figure 17 to edit the procedure variables before manually issuing the START command NOTE when using a two computer system the START BOXES command may need to be delayed
15. S Aversive Stimulator with Scrambler While one researcher would operate the output current dial on the ENV 414S trying to maintain a consistent 10UA rise every 5 seconds a second researcher would visually monitor the test subject through the SAC peephole for an aversive reaction to the current stimulus It was determined that there are several problems with this method that could be solved using other MED Associates hardware and software First maintaining a consistent current increase is much easier using MED PC to communicate with an ENV 413 Aversive Stimulator and ENV 412 Scrambler Second using a Video Monitor camera in place of a human eye allows for a more comfortable testing situation allows multiple researchers to confer over the subject animal s reactions and provides the ability to rewind and review the animal s reactions MED Associates Aversive Stimulation with Video Monitoring provides all of these features MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING Hardware The Aversive Stimulation with Video Monitoring package incorporates multiple hardware and software components shown in Figure 1 NOTE do NOT connect hardware until instructed by its driver installation program Figure 1 Aversive Stimulation with Video Monitoring Components Pr Seren The Aversive Stimulation with Video Monitoring Package requires the following hardware e ENV 008 FPU Quick Change Test Chamber e ENV 005 QD Quic
16. SIVE STIMULATION WITH VIDEO MONITORING Enter computer and camera names in Comments field Figure 9 MED PC Wizard Experiment Info Screen Subject o Experiment fo Group o Comment lestup SAC 1 Optional Custom Filename 2 Enter the Subject Experiment and Group parameters as necessary to identify the experiment In the Comments field type the Video Monitor computer name and the camera name each surrounded by double quotation marks 4 The format will be Comments Computer Name Camera Name Using the computer name seen in Figure 6 textxp and the camera name seen in Figure 7 SAC 1 the Comments field should read Comments testxp SAC 1 Press the Next button to show the Review Choices screen and be sure the Comments field is filled correctly Press the Next button to continue to the Session Parameters screen seen in Figure 10 l2 MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING Figure 10 MED PC Wizard Session Parameters Screen Cage Acclimation sec Step Duration sec Initial Shock Step Increment JA min 2 5 Maximum Current max 1000 Enter Session Parameters At the Session Parameters screen the Aversive Stimulation Ramp Up Model parameters default values are displayed Refer to Figure 2 for a graphical representation of the Ramp Up model Cage Acclimation default 5 0 seconds Cage Acclimation is the time
17. in seconds which the Initial Shock is applied to the grid floor before increasing Step Duration default 1 25 seconds Step Duration is the time in seconds between each successive rise in current Initial Shock default 0 0 uA Initial Shock is the current microamps flowing through the grid floor at the experiment start The Initial Shock current lasts for the time specified in the Cage Acclimation field Step Increment default 2 5 uA Step Increment is the amplitude of each current rise in microamps The minimum resolution of the ENV 413 is 2 5 therefore the minimum value allowed for the Step Increment variable is 2 5 pA Maximum Current default 1000 uA Maximum Current is the absolute highest current amperage that will flow through the grid floor 1000 microamps is equivalent to 1 milliamp The experiment will end and the video camera commanded to stop saving video when the maximum current is reached After pressing the Next button on the Session Parameters screen the Load More Boxes or Send Start Command Wizard screen will appear Before issuing the Start Command load any other boxes by repeating the above steps starting at choosing which box to load Choose I Want to Load More Boxes and the Wizard will take you there Be sure to enter the correct computer and camera name for each box in the Comments 13 MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING field of the Experiment Info screen
18. k Disconnect Grid Floor Connection Block Rat or ENV 005A QD Quick Disconnect Grid Floor Connection Block Mouse e DIG 729 USB Controller e ENV 413 Computer Controlled Aversive Stimulus Generator e ENV 412 Current Scrambler e Interface Cabinet houses the ENV 413 and ENV 412 cards e ENV 420 Current Tester optional e Sound Attenuating Cubicle e PC with peripherals Software The Aversive Stimulation with Video Monitoring Package requires the following software e DIG 729 Driver e SOF 735 MED PC application e SOF 842 Video Monitor application e SOF 732 4 Aversive Stimulation with Video Monitoring application e ENV 420 Current Tester Software optional 2 Aversive Stimulation with Video Monitoring Software Aversive Stimulation with Video Monitoring AvStimVideo is a MED PC MedState Notation MPC program with two main responsibilities e Control the current to the aversive stimulation chamber grid floor e Communicate with the Video Monitor software Figure 2 Aversive Stimulation Model Maximum Current Current uA Step Current Y Initial TC Current Time sec Acclimation Time Aversive Stimulation Control The AvStimVideo MPC program runs a simple ramp up model as shown in Figure 2 The model uses the following parameters e Initial Current default 0 0 pA e Acclimation Time default 5 seconds e Step Current default and minimum 2 5 pA e Step Time default
19. lation with Video Monitoring PDF will be copied to the MED PC IV Manuals directory The last code file to be copied will be a sample macro file called AvStimSample MAC It will be copied to the MED PC IV Macro folder Two Computer System Installation Procedure If MED PC IV and Video Monitor are running on separate computers the Video Monitor MED PC Interface components must be installed on the MED PC IV computer This is explained in Step 2 below Note steps 2 and 3 may be performed in any order 1 Install MED PC IV on the MED PC IV computer refer to the MED PC IV User s Manual for details Install Video Monitor MED PC Interface on the MED PC IV computer This will install the Video Monitor MED PC Interface components into the MED PC IV folder Insert the Video Monitor CD into the MED PC IV machine and click To install the Video Monitor MED PC interface click here See Figure 5 below Figure 5 Video Monitor MED PC Interface Installation Video Monitor i ASSOCIATES INE Welcome to Video Monitor Follow the directions for installing the Firewire camera drivers first After the Firewire cameras have been installed install Video Monitor To install Adobe Acrobat Reader click here To view the Video Monitor manual click here To install the Firewire camera drivers click here To install the Video Monitor MED PC interface click here To install Video Monitor click here Browse the CD Exit The installatio
20. n program will search for the MED PC IV program installed in step 1 Choose Yes when prompted to install to the found MED PC IV directory The install program will put the MED VM Interface DLL and VM lface HED files in the MED PC IV directory and VIDEO MPC in the MED PC IV MPC folder The installation program will then insert 1 VM IFACE HED into the User PAS file 3 Install Aversive Stimulation with Video Monitoring See step 3 in the Single Computer System Installation Procedure above 4 Install Video Monitor on the Video Monitor computer Refer to the Video Monitor User s Manual for details This step may be performed at any time in relation to the first three steps the two computer system the MED PC IV computer must have write file permissions on the Video Monitor computer It is recommended that both computers be a part of the same domain See your network administrator for details Again the single computer system is recommended to avoid this possible problem 4 Setting Up Aversive Stimulation with Video Monitoring To use Aversive Stimulation with Video Monitoring both MED PC and Video Monitor components must first be configured MED PC and Video Monitor may be running on separate computers In the example below MED PC is running on a computer named med pc and the Video Monitor computer is named testxp Video Monitor Configuration Find Computer Name In Aversive Stimulation with Video Monitoring
21. omputer PCI bus 6 device 2 Function 0 M Save Video 1 Cameras Max Current Card 1 Cameras Max Computer Cancel i imi Browse C Documents and Settings TestxP Timing Apply to all File Name Cameras Use Default Frame Rate fps Features Defaults 30 7 Brightness Supported Video Modes Uncompressed Video r 320 X 240 YUV 4 2 2 Mode 16bit pixel Bit Rate Kbps Complexity 320 X 240 Y Mono Mod Bbit pixel 640 X 480 Y Mono Sbitipixel Use Default 640 X 480 100 5 Y Mono Mode pi s is 480 256 250 5 Y Mono Mode Bbitjpixel 250 59 27 DER spara remanina File Quality Fit to Screen 50 Trigger Record For 2 r Manual Remote MED PC e g 2 Time Scheduler Annotation Set Default E 0 gt gt To configure Video Monitor start the application and bring up the System Settings dialog Navigate to the desired camera in the camera tree In Figure 7 the camera being used is SAC 1 Fill the checkbox in the On Off field to activate the camera display and fill the checkbox in the Save Video field Next choose the Remote MED PC e g option of the Trigger field to tell the camera not to start saving until commanded by MED PC Close the System Settings Dialog Last press the OK button The System Settings dialog will close and the SAC 1 camera s video stream will be displayed in a window titled Camera S
22. omputer and camera names and sending start command to MED PC A macro file is an optional step However creating a macro file for each testing scenario will greatly improve efficiency and is good practice to help define reliable and repeatable experiments CHAPTER 3 Software Installation Aversive Stimulation with Video Monitoring may be configured to operate on a single computer or two networked computers In the single computer system the three major software components Video Monitor MED PC IV and Aversive Stimulation with Video Monitoring are all installed on the same computer In the two computer setup MED PC IV and Aversive Stimulation with Video Monitoring software are installed onto one computer and Video Monitor on the other However due to the network security issues running on multiple computers may be difficult to configure It is recommended that MED PC IV Aversive Stimulation with Video Monitoring and Video Monitor all be installed on a single computer Figure 3 Single Computer vs Two Computer Setup Single Computer System Two Computer System Networked Computers The main difference between the single and two computer system installation procedures is the extra step of installing the Video Monitor MED PC Interface on the MED PC computer of the two computer system In the single computer setup the Video Monitor MED PC Interface is installed as part of the Video Monitor installation process
23. ower to the rack containing the ENV 412 and ENV 413 MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING CHAPTER 6 Video Playback and Analysis After the maximum current has been reached or a K pulse was issued the AvStimVideo procedure will end The ENV413 will stop transmitting current to the grid floor and the Video Camera will stop saving video Now it is time to analyze the saved video using the Video Monitor Playback tool Go to Video Monitor close the camera window pull down the Video Monitor File menu and choose Open Select the video that was just created by the AvStimVideo procedure in the File Open dialog and press the OK button Figure 15 Video Monitor Playback Dialog Video Monitor SAC 1_29Jan2007_11 20 23 wmv File View Window Help 9 SAC 1_29Jan2007_11 20 23 wmv BAE HO 7 ES Close Default v Alt Annotation _ Spinning Counterclockwise Spinning Clockwise Head Bob Freeze Sleep Rearing Grooming Stimulus Response Repeat Annotation Set View Annotations 00 27 466 Current 55 000 uA 28 699 03 15 499 The Playback window will appear as Figure 15 Notice that the AvStimVideo procedure has sent the Video Monitor program an annotation The annotation in the above example reads 0 00 27 466 Current 55 000 pA The AvStimVideo procedure sends a similar message at each step of the Aversive Stimulus ramp up model as well as the procedure start and stop
24. riables CTRL A option Press the Named Vars button to display the five procedure variables After making changes press the Issue button to finalize the edits Press the Close button to dismiss the dialog N MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING Figure 17 Change Named Variables Dialog Displaying Named Variables from Box 1 Cage Acclimation sec Step Duration sec Initial Shock Step Increment min 25 Named Vars Vars Refresh Maximum Current max 1000 Issue Close Help Additional Boxes to Update 12 Select All Deselect Al Display Data from Box If the values are not changed the defaults will be used Default procedure variable values e Cage Acclimation Period 5 0 seconds e Step Duration 1 25 seconds e Initial Shock 0 0 pA e Step Increment 2 5 HA e Maximum Current 1000 pA 1 milliamp 21 8 Using a Macro Loading boxes and setting experiment parameters can be simplified by using macro files MED PC can store and playback commands stored in a mac text file Macro files can be edited using any text editor e g Notepad or using the MED PC macro editor Macros menu Editor option Aversive Stimulation with Video Monitoring ships with a sample macro file AvStimSample mac shown in Figure 18 below Figure 18 Sample Macro File LOAD BOX 1 SUBJ 0
25. ring Insert the Aversive Stimulation with Video Monitoring distribution CD into the computer The screen shown in Figure 4 will appear If this screen does not appear use Windows Explorer to browse to the CD drive and double click the Autorun EXE file Figure 4 Aversive Stimulation with Video Monitoring Main Screen SOF 732 4 mei ASSOCIATES ING Aversive Stimulation with Video Monitoring To view the manual click here To install the software click here Browse the CD E Click To install the software click here and then click the Next button at each successive screen to choose the default installation settings The installation will search for the MEDPC IV EXE file to determine the MED PC IV installation directory If the installation program cannot find the MEDPC IV EXE file a prompt will appear to browse to the program Choose Yes when prompted to install to the found MED PC IV directory MULATION WITH VIDEO MONITORING The installation program will alter the User PAS file in the MED PC IV installation directory Two lines will be inserted e I AVSTIM SPT HED in the Place 51 Filename HED files here section e I AVSTIM_SPT BOD in the Place I Filename BOD files here section The installation program will copy the AvStim Spt BOD and AvStim Spt HED files to the MED PC IV installation folder The program then copies the AvStimVideo MPC file to the MED PC I VAMPC folder The user manual Aversive Stimu
26. to compensate for network communication lag Simply insert a line before the START BOXES 1 command such as DELAY 1000 without the quotes which will pause for 1 second 1000 milliseconds MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING CHAPTER 9 Troubleshooting No Session Parameters Figure 19 MED PC Wizard Session Parameters Screen Showing NO PARAMETERS If the Wizard Session Parameters screen has no information as seen in Figure 19 the Comments field was not properly entered Press the Close button on the Session Parameters dialog and restart the Wizard from MED PC File menu Wizard option Enter the Video Monitor computer name and camera name in double quotes E g SAC 1 23 MED ASSOCIATES INC AVERSIVE STIMULATION WITH VIDEO MONITORING USER I NPUT ERROR Message Figure 20 User Input Error Message MED PC IV File Configure View Macros Help AGO zl 8 edi 3 e Box Subject Expenm Group Load Program 1 2 EN 4 5 6 7 8 9 ER 12013 014 15 17 18 EET 20121 22 23 24 25 26 27 28 29 30 3 Closed SHOWS 11 15 SHOWS 16 20 SHOWS 21 25 SHOWS 26 30 SHOWS 31_35 USER INPUT ERROR 0 00 put computer and camera 0 00 in COMMENTS field 0 00 0 00 1 0 00 SHOWS 36 40 SHOWS 41 45 SHOWS 46 50
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