Hardware User`s Manual Active & passive avoidance box
Contents
1. 11 2 TRAYS CLEANING Trays contains the grids and collects faeces and urine they should be cleaned after each experiment in order to clean them you should remove them from the cage and then you can use a soapy solution and finally dry them Active amp passive avoidance box 22 Panlab Harvard 11 3 TRNASPARENT DOORS CLEANING nor alcoholic derived products otherwise stripes will appear in the WARNING In order to clean the transparent doors never use neither alcohol transparent plastic To clean the doors you can use a slightly wet cloth and then dry it with a dry cloth If they re too dirty you can wet the cloth with a soapy solution then remove foam with a wet cloth and finally dry them with a dry cloth 11 4 WALLS CLEANING To clean the walls you can use a slightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth 11 5 CLEANING THE ROUND TOP LIDS These lids are made of the same material as transparent doors so you should have the same precautions with them when cleaning never use alcohol or detergents derived from alcohol otherwise will appear stretch marks on the transparent plastic Active amp passive avoidance box 23 Panlab H Harvard 12 TROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION econo2. Rat 30 gr Mouse 7 gr SOUND Frequency Range 300 to 3000 Hz Precision 1 LIGHT By leds Not precise change Power 2 x 0 4W Light stimulus intensity can be modified by using jumpers in lamp board SW1 SW2 SW3 less light intensity factory settings more light intensity DOOR Time open closed Regulation open Regulation closed 0 65 mouse 0 755 rat Potentiometer PT5 Potentiometer PT6 CONNECTOR MAIN REMOTE Pin Function 2 Rxd 3 Txd 5 Gnd 7 Rts 9 Cts COMUNICATIONS OUTPUT Standard Interface RS232C Connector Transmission speed Delta 9 contacts connector 19200 bauds 8 bits no parity CONNECTOR LE2708 Pin 1 Function Door input Active amp passive avoidance box 2 Panlab I Harvard Apparatus 2 Sound Input 3 Light Input 4 Shock Input 5 Right Output 6 Left Output 7 Change Output 9 5V 15 Gnd CONNECTOR BOX Pin Function 1 Door 2 Door GND 6 Loudspeaker Active 8 Loudspeaker Gnd 10 Transducer left S 11 Transducer left S 12 Transducer Right S 13 Transducer Right S 14 Door control 16 Light Right 17 Light Left 18 Light 5V 20 Relays control 21 Relays 45V 23 Transducer Exc 10V 24 Transducer Exc 25 Gnd DIMENSIONS Control Unit Width x Height x Depth Weight 285mm x 70MM x 250mm 1 84 kg DIMENSIONS Cage MODEL Width Height without Height with Depth Weight door door LE918 mouse Active amp passive avoida
3. cover e SPEAKER There is a speaker for acoustic stimulus at the centre of the rear wall e PLATFORM The platform is the floor of the cage It contains the load cells one for each side e TRAY The tray collects animal droppings It can be removed for cleaning the cage It also has 4 legs that transmit weight to the load cell It contains the electrical grid GRID The grid transmits electrical shock to the animal The bars are equipped with a scramble of 6 elements to ensure that the animal receives shock Active amp passive avoidance box 12 Panlab H Aden e FRONTAL ACCESS There are 2 transparent frontal doors to introduce and remove animals to and from the cage e DOOR The door is an accessory When it is installed it has a motor to open close it LIGHT MOTOR BOX SHOCK CONNECTOR CONNECTOR GRID GRID CONNECTORS GRID Figure 10 Cage rear view There is a central box that contains all the connections at the rear of the cage e MOTOR CONNECTOR DBg female connector at the top side for connecting door motor e LIGHT CONNECTORS 2 female jacks at the top side for connecting the lights of each side BOXCONNECTOR DB25 male connector to connect cage to the control unit e SHOCK CONNECTOR DIN6 female connector used to connect the cage to the LE 100 26 Shocker or LE 2708 programmer e GRID CONNECTORS 2 DBg female connectors located on the bottom for transmitting shock to each grid There is a speak
4. moria e Check the condition of the fuses Ensure that the cable DB2s connects the cage and control unit Check that trays and grids are correctly placed If the cable is connected and all is correctly placed this means both load cells are damaged The two white position leds are blinking The load cell is damaged or One of the white position leds is blinking blocked Check that door cable is connected Check that DB25 cable is connected Check that Ready led is on if it s blinking system still is auto balancing and door will not work The door does not open Check that DB25 cable is connected Th tic stimulus d t k e acoustic stimulus does not wor cech dee Ready edison Check that light stimuli cables are connected Check that DB25 cable is connected Check that Ready led is on The light stimuli do not work Check that Ready led is on Check that grids and trays are correctly placed Check that the RAT MOUSE switch is in the correct position Rat weight threshold detection is 3ogr if you work with mice the equipment will not be able to detect them The control unit does not detect animal position Active amp passive avoidance box 24 Panlab Harvard Check that DIN6 cable is connected between the shock generator and cage Check that the DBg flat cables are connected between the grids and the cage Check that jack mono to green and black bananas is connected between cont
5. remains continuously on indicating that the system is ready to work e FREQUENCY 10 turn potentiometer used to adjust frequency of the acoustic stimulus from 300 Hz to 3 kHz e VOLUME 10 turn potentiometer used to adjust acoustic stimulus volume e STIMULI Button used to test acoustic and visual stimuli in the cage e BOX STATUS Seven leds that show box status when light and sound stimuli are on when door is closed and animal position Box Status o Visual stimuli on right side 1 and left side 2 yellow colour leds o Acoustic Stimuli 3 yellow colour led o Door closed 4 red colour leds 6 4 7 o Animal position left 6 or right 7 white colour leds Figure 7 Box Status leds e DOOR Button used to test the door It will open or close depending on the original position Active amp passive avoidance box 10 Panlab H Harvard 7 2 LE9oo REAR PANEL BOXID SHOCK RAT MOUSE POWER Main Remote LE 2708 EN o were KA MAIN REMOTE LE 2708 BOX C Rated Voltage 90 260 V AC 50 60 Hz 18VA Figure 8 Control unit Rear Panel e BOX ID Decimal selector used when the system is controlled by computer When several cages are connected to the same computer there cannot be 2 cages with same ID number e MAIN DB9 female connector used to connect LE to the serial port of the computer When the computer controls several control units conn
6. sound and door can be tested using the buttons labelled mu and CDn the front panel using the 2 dials on the front panel 7 If you are working with a rat cage select Rat on the rear panel selector If you working with a mouse cage select G nd Mouse on the same selector 8 Place the animal in the cage 9 Follow the Shutavoid program or LE 2708 indications for experimenting with the animal read related manual depending on the equipment you have purchased 10 Remove the animal form the cage once the experiment is ended and clean grids and trays 11 Return to point number 8 to work with the next animal 12 Oncethe experiment has finished turn off all equipment and clean the cage Active amp passive avoidance box 20 Harvar Panlab Horvora 10 2 AUTOMATIC ERROR WARNING The system checks if the cable between the rear panel connector labelled Box and the cage is connected If the system detects this error the 2 white position leds in the Status box on the front panel see Figure 7 will flash The system is then waiting for the problem to be solved cable connected After solving the problem the system must be turned on again Once the cable is connected if the system detects that a load cell is damaged it will warn the user with a flashing white led in the position that belongs to this load cell see Figure 7 Active amp passive avoidance box 21 Panlab 11 RECOMMEND
7. ATIONS 1 It will be necessary to periodically clean the experimentation cage 2 Trays and grids can be removed for cleaning Prior to removing them turn off the control unit and disconnect the flat cables that join the cage with the 2 shock grids 3 Once grids and trays have been returned to the cage check that they are correctly placed the four legs of the trays must be in the load cell supports to correctly transmit weight to it 4 System auto calibration must be done without animals on the grid during auto calibration the system detects the grid and tray weight and balances it to detect animal weight 5 Although not required it is better to wait 10 minutes after having turned on the equipment before placing animals on the grid the system will reach a permanent thermal state in this period 11 1 GRIDS CLEANING When there is dirt in the grids the electrical shock flows through it This will produce erroneous data during the experiment because the animal behaviour to the shock negative reinforcement will not be correct it will not receive punishment In order to clean the grids you can use water and soap and then you must dry it Grids sets can be removed from the trays in order to clean them Be sure to dry well the DB9 connector otherwise contacts may rust with the time Special care must be taken in cleaning the plastic between bars because urine is a good electrical conductor and current would flow through it
8. Hardware User s Manual Active amp passive avoidance box Shuttle box References L E916 76 0250 LE918 76 0251 Version V28 10 2014 B Panlab s l u International Calls 34 934 750 697 p n b H A Harvard C Energia 112 Domestic Call 934 190 709 APPAUTUs 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab H Harvard 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C AN touched pese s a mee pror 19 mr e e end operative life Waste Electrical and Electronic Equipment Directive WEEE A 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures whic
9. MC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Class A equipment is intended for use in an industrial environment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference N when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if WARNING not installed and used in accordance with these instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Active amp passive avoidance box Panlab Ades 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault
10. e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from the power inlet Active amp passive avoidance box Panlab Alas 2 Open fuse holder by pulling the flange with a regular screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipme
11. e o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuig o para a protecc o do ambiente PG Active amp passive avoidance box 30
12. ected in cascade the serial port of the computer is connected to the MAIN port of the first control unit and the MAIN port of the next control unit is connected to the REMOTE port of the previous control unit The REMOTE port of the last control unit is left free e REMOTE DBo male connector used to connect the control unit to the MAIN port of next control unit when several cages are controlled by a computer The REMOTE port of the last control unit is left free e LE 2708 DB15 female connector used to connect the control unit to the LE 2708 programmer when control is not done by computer e BOX DB25 female connector used to connect the control unit to the experimentation cage It transmits load cell signals stimuli and door activation signals e SHOCK It connects the LE with the LE 100 26 Shocker It sends the signal to activate the shocker e RAT MOUSE Selector used to set the model of cage being used rat or mouse POWER Power inlet main switch and fuse holder Active amp passive avoidance box 11 Panlab H Ade 7 3 EXPERIMENTATION CAGE ROUND TOP LID MOTOR PLATFORM TRAY GRID DOOR FRONTAL ACCESS Figure 9 Cage front view e LIGHT There are 2 lights one on each side of the cage for visual stimulus e ROUND TOP LID There is a round transparent top lid on each side of the cage In order to remove it you should turn the round lid till the notches of it match with the holes in the
13. er inside the connection box on the rear panel of the cage It is used to give acoustic stimulation to the animal Active amp passive avoidance box 13 Panlab H Reh 8 EQUIPMENT CONNECTION 8 1 CONTROL BY COMPUTER Box ld gt Y Remote LEM G goooo pocos D 2000000000000 100 0 1 1000000000000 Made in Spain by Paniab s i LE 100 26 Figure 11 Connections schematic for control by computer Active amp passive avoidance box 14 Panlab ri The connections and cables necessary for controlling the Shuttle Box by computer are the following FROM CABLE LIGHT left right CAGE Jack cable H GRID left DBg flat cable 6 LE MAIN MAIN PCSERIAL PORT RS 232 cable LE 900 Shock LE 100 26 External Jack to green and Time amp GND black banana cable A computer is able to control up to 8 cages When working with several cages connection between control units and the computer is as follows an example of connection of 3 cages is shown in Figure 12 Figure 12 Example Control of 3 cages with the same computer This cable is optional Shuttle Box experiments always use an opened door Active amp passive avoidance box 15 Panlab Havers Some important points must be followed when working with more than one cage e A
14. h may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Active amp passive avoidance box I Harvar Panlab E 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam
15. le LE 2708 SHOCK CAGESHOCK DIN6 cable 6 LE2708CAGE LEgoo LE2708 DBiscable This cable is optional Shuttle Box experiments always use an opened door Active amp passive avoidance box 18 Panlab H Harvard 9 AUDIO STIMULUS The audio stimulus can be modified with the two 10 turn dials on the front panel e Frequency This dial adjusts frequency from 300 Hz to 3 kHz e Volume This dial adjusts sound volume The frequency dial is linear and frequency can be calculated with the following equation f 270 D 300 Where f frequency in Hertz D Value of the frequency dial Relation Dial Frecuency gt 2 Pu LL Figure 14 Graphical representation of frequency vs value of the dial Figure 14 shows the graphical representation of frequency vs the value set in the dial Active amp passive avoidance box 19 Panlab Harvard 10 WORKING WITH THE EQUIPMENT 10 1 PROCEDURE The following procedure must be followed to work with the equipment 1 Connect all the cables see chapter 8 in this manual 2 If you work with the program Shutavoid and several control units choose pa a different ID for each cage 0 3 Connect control unit to AC network and turn it on a few seconds until the O Ready led is continuously on It will blink during auto calibration 5 Adjust Volume and Frequency of acoustic stimulus Volume de Light
16. le Box experiment is to test animal response to signals acoustic and visual in the case of this appliance Figure 5 Shuttle Box There are 2 cage models the smaller unit LE 918 is designed for mice and the larger one LE 916 is designed for rats Control unit LE can work with both cage models by means of a switch labelled BOX on the rear panel Rat Mouse Animal position detection is based on load cells To do so the grid is placed on a tray with 4 legs that transmit force to the load cell Adjustment of load change detection is automatic There are two options to control the experiment e Connect the LE goo to the LE 2708 programmer through the DB15 female connector labelled LE 2708 each programmer can control 1 cage e Connect the LE to the serial port of computer and use the Shutavoid software each computer can control up to 8 cages The shock is supplied to the cage using the LE 100 26 shocker Optionally a motor can be installed to open close the door that separates both sides Active amp passive avoidance box Panlab Harvard 7 EQUIPMENT DESCRIPTION 7 1 LE 900 FRONT PANEL READY BOX STATUS L Frequency Volume m AN qm NN SHUTTLE BOX CONTROL E 900 EET Panlat HARVARD FREQUENCY VOLUME STIMULI DOOR Figure 6 LE 900 front panel e READY Green led When the LE 900 is turned on it blinks for 20 seconds indicating that system is auto calibrating After this period it
17. lltheunits must have different IDs so that computer can identify them e It is not necessary for all ID numbers to be correlative For example it is as correct to work with cages 1 2 and 3 as it is to work with cages 1 5 and 7 e t is not necessary for cages to be physically located in order of their ID numbers For example it will be correct to work with cages 1 2 3 1 3 2 2 1 3 2 3 1 3 1 2 Or 3 2 1 e The computer serial port is always connected to the MAIN port of the first control unit e The REMOTE port of each control unit is always connected to the MAIN port of the next control unit e The REMOTE port of the last control unit is left free Active amp passive avoidance box 16 Panlab H Ade 8 2 CONTROL WITH LE 2708 Each LE 2708 can only control one cage The necessary connections are shown in the next figure LE 2708 TIMERS End pulse End pulse End pulse Tras Start control Stan Start Start O O O A HQ mes puse nes puse O O CHO res putse O LJ Output O LJ Og Output Stop Stop AC 5080 Ha VIVA Figure 13 Control with LE 2708 Active amp passive avoidance box 17 Panlab Harvard The connections necessary to program an LE 2708 are not detailed in this manual If you need further information please read the LE 2708 Quick Procedure The necessary connections and cables are listed in the next table FROM CABLR LE BOX CAGE BOX DB25 cab
18. lt Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique PG Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leis
19. nce box 28 Panlab E Hoarverd DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto SHUTTLE BOX CONTROL Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 916 LE 918 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Rest
20. nt and contact technical service N For electrical safety never open the equipment The power supply WARNING has dangerous voltages Active amp passive avoidance box Panlab H 5 TABLE OF CONTENTS 10 10 1 10 2 11 11 1 11 2 11 3 11 4 11 5 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION LE 900 FRONT PANEL LEgoo REAR PANEL EXPERIMENTATION CAGE EQUIPMENT CONNECTION CONTROL BY COMPUTER CONTROL WITH LE 2708 AUDIO STIMULUS WORKING WITH THE EQUIPMENT PROCEDURE AUTOMATIC ERROR WARNING RECOMMENDATIONS GRIDS CLEANING TRAYS CLEANING TRNASPARENT DOORS CLEANING WALLS CLEANING CLEANING THE ROUND TOP LIDS Active amp passive avoidance box Harvard Apparatus 10 10 11 12 14 14 17 19 20 20 21 22 22 22 23 23 23 12 TROUBLESHOOTING 13 PREVENTIVE MAINTENANCE 14 TECHNICAL SPECIFICATIONS Active amp passive avoidance box Panlab H Harvard Apparatus 24 26 27 Panlab H je 6 INTRODUCTION The Shuttle Box or Avoidance and Escape Cage is designed to study and monitor the movements of an animal between two cages separated by a door resulting from the negative reinforcement caused by electrical shock on both sides of the cage and the conditioned stimuli that help it to escape from the discharge Thus the goal of a Shutt
21. parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category II over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Active amp passive avoidance box Panlab Harvard PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and E
22. plies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tiar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de aparatos usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente I
23. riction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class A Safety of machinery EN ISO 12100 2010 This equipment complies with the limits for class A equipment in accordance with CISPR 11 definition and is classed as a Class A digital device pursuant to CFR Title 47 part 15 of the FCC Rules and is intended to be used in an industrial environment En consecuencia este producto puede incorporar el marcado CE Consequently this product can incorporate the CE marking En consequence ce produit peut incorporer le marquage CE En representacion del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canalis 3 Sa I General Manager MEIN Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 15 10 2014 Active amp passive avoidance box 29 H Harvard A Apparatus Panlab GB Note on environmental protection GB After the implementation of the European Directive 2002 96 EU in the national legal system the following ap
24. rol unit and LE100 26 Shocker Check that SHOCK CALIBRATION switch in the shock generator is in the SHOCK position Check that the INTENSITY knob in the shock generator is set to a The animal does not receive shock value higher than omA Check that the grid is clean urine and excrements can conduct current Make sure your equipment is connected to PC via RS 232 Check that Shutavoid settings so that the serial port is correct Restart the equipment and the PC The equipment does not communicate to do a RESET in communications with Shutavoid Check in the rear panel of control unit that the ID number is the same that the one set in Shutavoid Active amp passive avoidance box 25 Panlab 13 PREVENTIVE MAINTENANCE EXPERIMENT MONTHLY GRIDS CLEANING TRAYS CLEANING Gowne M CLEANING TOP LIDS CLEANING mausam WE Bae e M oo PLACING Active amp passive avoidance box Harvard Apparatus 26 Harvard Apparatus Panlab 14 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum Power Conducted Noise Universal input 100 240 VAC 50 60 Hz 2 fuses 5x2omm 2A 250V Fast 18 W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating Relative Humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing POSITION DETECTION
25. ten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt pe Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente PG Nota em Protecc o Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo sao definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instruc es ou a embalagem indicam qu
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