AMPHASYS AG TECHNOPARK® Lucerne Platz 4 CH
Contents
1. Ampha Z 30 IMPEDANGE FLOW CYTOMETER USER MANUAL AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland e phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO Content MN 3 VANTE NNN 3 1 2 Delivery and Inspection rrnrrnnnrnnnrnnnrnnnrnnnrnnnrrnnrnnnrnnnrnnernnsrnnnrnnsennsrnnnennenneenneennennennennsennee 3 Bebe 91911110 dei EEE EE 4 FAP 4 21 Electrical Salet EEE 4 AA oe allez EE ela 6 SG E EEE EEE NE EEE NE E A 6 3 Setting up Ampha EEE EE 7 Sot beatza ea RAE 7 3 1 1 Computer d 7 Sallie SOI ALS DP 7 PE EEE EE 7 Sa Erlea Ga eN 7 3 2 MEASUEMENN cccccccecccsecceeecececeecceeeceuecsueceeeceeesaueceecseeseuesseeseeetseesseetseeseresseeseeeseeeneeeneeenes 8 NN 8 4 ETT 0 1 La 10 4 1 Change and Replacement of Fluidic Set rrrrrnnnrnnanrnnnnnnnnrnranrnnnnnvanrnnnnnnnnrnnanennnnnnanennnennnene 10 4 1 1 Change from OLD to NEW Tubing Set rrrnanrnnnnrnnnnnnnnennnnnrnnrnnnnennnrnnnnennnrnrnennnnnnnsennnne 11 4 1 2 Replacement of New Tubing Set ss 12 4 1 3 Replacement of Old Tubing Set arrrranennnnnvanennnnnnnnrnvanennnnnnanennnnnnnnrnnanennnsnnanennnnrnnsnnnne 13 4 2 Replacement of Water Bottle Filter rrrnrrnnrrnnnrnnnevnrrnnnrnnnrvnnevnnrvnnrnnnrnnnnnnrnnnnnnnnnnnnennnennnen 14 2 TN NN 14 5 Troubleshooting and technical support sanea 17 5 1 Troubleshooting arrrnnnrvanrrrnnrnnnrnranrnnnnnnanrnnnnrnnnrnnanennnnnnansnnanrnnn2. Attention sample will be washed away during the following steps Fluidic problem Fluidic blocking Use expert window check liquid bottles Water bottle is full but tubing is empty check liquid bottles take out chip put pump speed to 100 rom make sure to have liquid from sample loading if not select right valve to switch to system water Check left valve uncheck right valve check pump to Is water liquid bottle run dry Check if fitting to the bottle is fixed Fig 7 check connection of water filter in the bottle to the tubing Fig 8 C D perform Rinsing Is water liquid bottle run dry Follow air gapes in the tubing to see liquid running liquid comes out at the chip inlet O ring Follow air gapes in the tubing amp to see liquid User Manual Ampha ZOO If yes go to problem solved problem solved EN C1 1 problem solved C1 C1 1 fill the water bottle and run 3 times Rinsing problem solved fill the water bottle and run 3 times rinsing problem solved dry chip area put in cleaned chip uncheck valve and go on with your measurements Yes put valve and pump selection If no go to contact Amphasys B2 contact Amphasys Use another chip broken chips discard for chips with problem contact Amphasys C1 2 C1 2 C2 1 C2 2 C2 3 C2 4 C2 5 Use another chip for chips with problem contact Amphasys D2 Contact Amphasys for further inst
3. The AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 12 User Manual Ampha ZOO peek nut should be loose at the tubing Insert the tubing with the ferrule in place into the receiving port and while holding the tubing down firmly into the port tighten the nut finger tight Push now the right silicone tubing on the barb adapter above the tube holder Fig 7F and push the left longer silicon tubing on the barb adapter in the instrument wall Fig 7G Be sure that the left longer silicone tubing has an easy loose knot not pressing or squeezing the silicone tubing Fig 7E H Place this tubing as in Figure 7H don let it hang down Start the instrument and perform 2 to 3 times the rinsing program to fill the lines 4 1 3 Replacement of Old Tubing Set Before removing the old tubing set make sure that the peristaltic pump is not running by checking the software or looking at the pump head For changing the tubing set unscrew the three peek fittings Fig 8A remove the tubings from the valves Take out the pump head by pressing the left and right clamp and lifting the head Fig 6 and 8B Place new tubing set with the pump head on the pump axis and press down that the clamps snap into place Fig 80 To screw the fittings into the corresponding ports check that the blue ferrule is flush with the tubing Fig 8D inlet The peek nut shoul
4. Temperature 16 32 C Humidity 10 90 relative non condensing Power 24V DC 10 max 3A lt 90 W Table 1 Specifications of Ampha Z30 2 Hazard and Precaution This handbook contains information and warnings that must be followed by the user to ensure safe operation of the instrument and to maintain the instrument in a safe condition Possible hazards that could harm the user or result in damage to the instrument are clearly stated at the appropriate places throughout this handbook Before using the instrument it is essential to read this handbook carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of the instrument Advices given in this handbook are intended to supplement not supersede the normal safety requirements prevailing in the user country Carry out the maintenance regularly in accordance with the operating instructions Amphasys will charge for repairs that prove to be required due to incorrect maintenance 2 1 Electrical Safety To ensure satisfactory and safe operation of the instrument it is essential that the neutral line power cord is connected to true electrical earth ground When working with the instrument Make sure the line power cord is connected to a line power outlet that has a protective conductor earth ground Do not attempt to make any internal adjustments or replacements AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D
5. clogged chips see instructions below e Warning Do not apply high pressure and do not use liquids with pH lt 6 and pH gt 8 alcohols or any corrosive agents Doing so can lead to the destruction of the chips Figure 11 Chip Wash Station A placing of chip B rinsing from inlet hole C rinsing from outlet hole How to de block clogged chips e When chips are clogged by dust cells or any other particles we recommend the cleaning procedure with the chip wash station Fig 11 e Cleaning should be started with lt 0 5 um filtered high purity water If necessary other detergents can be used see Tab 5 Don rinse with any organic liquids like alcohols e Checking the chip under a microscope usually helps to identify the cause of the clogging When there is a blocking between inlet and the sensing channel rinse from outlet hole when there is a blocking between outlet hole and sensing channel rinse from inlet hole e After special cleaning procedures always rinse the chip with water from the inlet hole Blow out the water by pumping air through the chip wash station Detergent Usage U lt 0 5 um filtered high purity water e general cleaning liquid lt 0 5 um filtered 2 Neutracon or e chip cleaning of persistent biological cell blockings or solutions with dishwashing liquids residues in channels holes e detergents should be used for maximal 10 minutes because electrodes could be eroded after longer exposure lt 0 5
6. um filtered AmphaClean e chip cleaning of dirt and persistent biological cell blockings or residues in channels holes Table 5 Chip cleaning detergents AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 15 User Manual Ampha ZOO Additional tips for still blocked chips e After cleaning with water let the chip dry out overnight and try to clean again e Try with ultrasonic bath take out the chip from the chip holder put it for 1 minute in the bath containing clean water put it back in the chip holder and try to clean again e I you know what kind of dirt is blocking the channel you may try to dissolve the dirt with an appropriate solution detergent Keep in mind that the chip channel structure and electrodes can be destroyed by certain chemicals In case of doubt please contact Amphasys e Cleaning of the chip holes and channel with water or air and gentle pressure can be generated by a normal plastic syringe with a small silicon tubing on the top Pressing this at the hole and either soaking or pressing can remove dirt Fig 12 Figure 12 Syringe with silicone tubing for chip cleaning left and soaking at the inlet hole right AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 16 5 Troubleshooting and technical support 5 1 Tr
7. 0 rpm 80 501 100 um D00000 120 x 120 um 2 11 300 rom 60 501 100 rom E00000 250 x 250 um IK 1i 500 rpm 3001 350 rpm Table 3 AmphaChips settings for fluidic set up AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO 3 2 Measurement All information about AmphaSoft is supplied in the separate AmphaSoft SW User Guide 3 2 1 Start e Check liquid bottles The front bottle normally contains deionized water the bottle at the side is for waste liquid and should be possibly empty at the beginning of a test series EO a MK i A e i EO i Ea E ATT D D SES Figure 3 A Instrument B Interface with chip C Software shortcut icon on desktop e Switch on the instrument the power switch is located at the back of the instrument Turn on the laptop PC Make sure that the USB cable is correctly connected to your device and the USB dongle with the AmphaSoft software license is plugged in e Start the software fAmphaSoftoby double clicking the icon Fig 3C e Select your user mode fOnlinedif you are connected to the EEE instrument and plan to perform a measurement or fOfflineQ if n you want to load and analyse data obtained from a former experiment Fig 4 GZ Online Offline Figure 4 Selection of online or offline user mode e AmphaSoft is started with a Main GUI Fig 5 All info
8. 4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO Do not operate the instrument with any covers or parts removed If water or reagent has spilled inside the instrument switch off the instrument and disconnect it from the line power supply Contact Amphasys AG or the authorized distributor Servicing should be carried out only by Amphasys AG or the authorized distributor If the instrument becomes electrically unsafe for use make the instrument inoperative and secure it against unauthorized or unintentional operation Contact Amphasys AG or the authorized distributor The instrument is likely to be electrically unsafe when it shows visible damage the line power cord shows signs of damage it has been stored under unfavourable conditions for a prolonged period or it has been subjected to severe transport stresses WARNING ELECTRICAL HAZARD The power supply is connected either at the rear of the instrument or inside the instrument housing arrows in Fig 2 The instrument is supplied with a 9OW industrial adaptor power supply which provides output voltages of max 24V Therefore no lethal voltages are supplied inside the instrument However any interruption of the protective conductor earth ground lead of the AC power supply cable or damage of the cable insulation is likely to make the instrument dangerous Intentional interruption is prohibite
9. AmphaSoft or Communication error Communication error closing process not finished wait always some seconds before restart AmphaSoft Communication error Communication error wrong data saving Electronics error Communication error Electronics error Communication error Fluidic problem Chip blocking Fluidic blocking Measurement with special buffers Ficoll sugars Bad electronic connections Chip broken Chip blocking Fluidic problem Measurement with special buffers Ficoll sugars Chip broken Contact Amphasys Table 6 Problems with the system and during measurement Cause A 1 Communication error 2 3 Communication error speed Solution What to do Success If yes go to question exit AmphaSoft and turn off instrument and PC turn on instrument and PC and restart AmphaSoft check USB connection from instrument to PC Close the popup windows by confirming and press ESC restart AmphaSoft If exit of AmphaSoft is not possible with normal closing stop process with TaskManager User Manual Ampha ZOO No in Tab 7 A A3 A4 U gt PW FW TI D GOGOA TT TIO O If no go to problem solved A2 A1 contact Amphasys problem solved A1 AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 17 1 1 1 2 2 1 2 2 2 3 2 4 2 5 Cause Communication error low pro
10. after the last measurement with AmphaClean solution as a cleaning detergent Menu gt Tools gt Instrument Cleaning In order to prevent system failures due to clogging porous tubing amp or other problems with the fluidic system it is necessary to exchange the peristaltic pump head including the tubing amp the fluidic bottle filter and O rings at least every two to three months Shorter replacement cycles may be necessary if the instrument is heavily used Omitting the replacement of the fluidics set may cause limited warranty The following table lists the parts and reagents that are required for regular maintenance tab 4 More details are available at www amphasys com community Table 4 Accessories and replacement parts for maintenance 4 1 Change and Replacement of Fluidic Set The Fluidic Set see Fig 6 is supplied with different tubing dimensions S M or L depending on the applications you are running on your device The Fluidic Set was optimized and the Silicon tubingG and fittings have been adapted Fig 6 Clamp r P Adapter Figure 6 Fluidic Set including pump head left NEW right OLD set Clamp l AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 10 User Manual Ampha ZOO 4 1 1 Change from OLD to NEW Tubing Set With the change of the fluidic sets from old to new set there is also a change of t
11. cessing Electronics error Electronics error Chip broken Chip blocking Solution What to do During measurement save data only locally at the laptop don use server connections for saving exit AmphaSoft and restart it exit AmphaSoft and turn off instrument and PC turn on instrument and PC and restart AmphaSoft Chip was tested is broken or no solution found inform Amphasys take out chip put chip in rinsing device or look directly at it under the microscope rinse the chip from the front hole with the syringe and 0 2 um filtered distilled water rinse the chip from the back hole with the syringe and 0 2um filtered distilled water use other filtered liquids to rinse from the back or from the front mild detergent or alcohol see Tab 4 fill chip with AmphaClean incubate for 30 min maximum rinse thoroughly with water wait until the chip channels are dry push air through the chip with the syringe fill the chip with water put it in distilled water in an ultrasonic bath for 1 to 2 minutes Success question Measurement works now with normal process speed and without any warnings or error messages no blocking visible in front of the narrow channel water comes out at the tube connected to the chip outlet hole water comes out at the tube connected to the chip inlet hole liquid comes out at the tubes connected to the chip holes all cell derived blockings are dispersed
12. d Use only the supplied power supply Any other power supply might lead to an incorrect functioning of the instrument or even to its damage 10 max 3A ohasys AG 6039 Root D4 Power Input Digital I O 24VDC 3A SB Ethernet Figure 2 Ampha Z30 rear side Arrows indicate the plugs for the connection of the power supply AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO 2 2 Biological Safety WARNING SAMPLES CONTAINING INFECTIOUS AGENTS If you use infectious agents with this instrument handle such samples with the greatest of care and in accordance with the required safety regulations The responsible body e g laboratory manager must take the necessary precautions to ensure that the surrounding work place is safe and that the instrument operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Materials Safety Data Sheets MSDS or other regulatory documents Disposal of wastes must be in accordance with all national state and local health and safety regulations and laws WARNING WASTE DISPOSAL Waste containers may contain hazardous chemicals or infectious agents from the process Such wastes must be collected and disposed of properly in accordance with the local safety regulations Refer to your local safety regulations for proper disp
13. d be loose at the tubing Insert the tubing with the ferrule in place into the receiving port and while holding the tubing down firmly into the port tighten the nut finger tight Place the right fitting in the sample holder the left fitting in the inner port Fig 8D Press the both silicone tubing amp ending in a Y connection in the inner valve ports and the both silicone tubing with direct connection in the outer valve port Fig 8E F Screw the middle fitting coming from Y connector in the outer port of the instrument Fig 8F Start the instrument and perform 2 to 3 times the rinsing program to fill the lines A Figure 8 Change of old peristaltic tubing set including pump head AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 13 User Manual Ampha ZOO 4 2 Replacement of Water Bottle Filter We recommend to replace the fluidic bottle filter Fig 9A quarterly together with the fluidic set For changing the bottle filter open the bottle and take it out from the instrument Fig 9B Unscrew the filter from the plastic fitting Fig 9 C D Check that the tubing in the fitting has overhang of about 3 mm Fig 9D Then finger tighten the fitting into the new filter Fig 9 B E place the filter back in the water filled bottle and close the lid Perform 3 times the rinsing program to fill the filter and the tubing A Figur
14. e 9 Change of fluidic bottle filter 4 3 Chip cleaning Chip insertion in chip holder e Keep the chip holder ready with the Amphasys sign facing upside e Take the chip with the holes facing down the Amphasys logo is in the lower right side e Put the chip into the holder by carefully pressing the lateral clip until the chip is snapping in its place Fig 10 Figure 10 Placing the chip into chip holder AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 14 Jo User Manual Ampha Z30 Chip cleaning and storing e The chips are delicate microfluidic devices They should be handled with care cleaned regularly und stored in a dry and dust free box in the dark e The chip surface should be cleaned with a non fuzzing tissue e g Anticon wipes and alcohol if needed Use of fuzzing tissue may cause clogging of the microfluidic channel e Chips should be cleaned immediately after use There are flush rinse and wash cycles that can be used while the chip is inserted in the instrument see AmphaSoft User Guide Alternatively the chips can be washed with filtered lt 0 5 um high purity water or mild detergents see below in the chip wash station Fig 11 using a syringe luer lock connection e When using the chip wash station general chip cleaning of non blocked chips should be done from the inlet hole to the outlet hole For de blocking of
15. g things dry electrical connections on chip and in the instrument with the special clean room towel provided clean electrical connections on chip and in instrument with Acetone Examine electrical spring contacts in the instrument to find out whether they are bent and do not contact the pads on the chip any more or whether they are even broken Try to bend them carefully to a good chip contacting position Put in another chip and try again question running liquid comes out at sample loading Liquid comes out at unscrewed fitting Water comes out at chip inlet O ring Liquid comes out at unscrewed fitting Visible problems or breaks Liquid drops at all sides EW adjust pump speed Cell sedimentation is reduced Cell sedimentation is reduced to an acceptable level Measurements are okay Measurements are okay Measurements are okay Measurements are okay back E3 1 E3 2 Put all fittings back to their port E2 Yes screw fitting E3 2 new tubing set necessary Yes screw in system E2 problem solved F2 1 problem solved problem solved problem solved problem solved problem solved The first chip was broken E4 Blocked check valve exchange or cleaning necessary contact Amphasys E4 1 E4 2 new tubing set necessary F2 2 contact Amphasys G1 3 G2 Try with another chip If measurements are bad contact Amphasys Table 7 Possible solut
16. he Ampha Z30 internal fluidic by removing the check valve For details you will be contacted by Amphasys or please contact Amphasys Before removing the old tubing set make sure that the peristaltic pump is not running by checking the software or looking at the pump head For changing the tubing set unscrew the three peek fittings Fig 8A from the old set and remove all tubing from the valves Take out the pump head by pressing the left and right clamp and lifting the head Fig 6 and 7C Place new tubing set with the pump head on the pump axis and press down that the clamps snap into place Fig 70 Screw in the two new Barb Adapters Fig 6 7 A B Press both silicone tubings ending in a Y connection into the inner valve ports and the two silicone tubing amp with direct connection into the outer valve port Fig 7C E H Screw the middle fitting coming from Y connector in the outer port of the instrument Fig 7F To screw the fitting into the corresponding port check that the blue ferrule is flush with the tubing Fig 7D inlet The peek nut should be loose at the tubing Insert the tubing with the ferrule in place into the receiving port and while holding the tubing down firmly into the port tighten the nut finger tight Push now the right silicone tubing on the barb adapter above the tube holder Fig 7F and push the left longer silicon tubing on the barb adapter in the instrument wall Fig 7G Be sure that the left longer s
17. ilicone tubing has an easy loose knot not pressing or squeezing the silicone tubing Fig 7E H Place this tubing as in Figure 7H don let it hang down Start the instrument and perform 2 to 3 times the rinsing program to fill the lines AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 11 User Manual Ampha ZOO i Figure 7 Change of new peristaltic tubing set including pump head 4 1 2 Replacement of New Tubing Set Before removing the old tubing set make sure that the peristaltic pump is not running by checking the software or looking at the pump head For changing the tubing set unscrew the peek fitting Fig 7D drag down the both silicon tubing amp from the barb adapters Fig 7F D and remove all tubing amp from the valves Take out the pump head by pressing the left and right clamp and lifting the head Fig 6 and 7C Place new tubing set with the pump head on the pump axis and press down that the clamps snap into place Fig 7C Press the both silicone tubing amp ending in a Y connection in the inner valve ports and the both silicone tubing amp with direct connection in the outer valve port Fig 7C E H Screw the middle fitting coming from Y connector in the outer port of the instrument Fig 7F To screw the fitting into the corresponding port check that the blue ferrule is flush with the tubing Fig 7D inlet
18. ions of problems listed in Tab 6 AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 19 Ke y User Manual Ampha ZOO 5 2 Limited Warranty Limited warranty period is one 1 year from first operation of the Product by Buyer It covers software and firmware and defects in material and workmanship under normal use and does not apply to ordinary wear and tear For detailed information refer to the warranty information at www amphasys com 5 3 Extended Warranties Extended warranties are available Visit www amphasys com for information 5 4 Technical Support For technical support contact Amphasys AG Technopark Lucerne Platz 4 CH 6039 Root D4 Switzerland Phone 41 41 541 91 20 Email info amphasys com 5 5 Ordering Information For a complete list of parts and reagents please visit www amphasys com AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 20
19. meter System 1 2 Delivery and Inspection The Ampha Z30 system includes AmphaSoft for controlling the instrument data acquisition and data analysis which runs on a Windows based laptop PC with an USB dongle for the software license Carefully inspect all package boxes upon receipt of the cytometer If there are any signs of mishandling or damage file a claim with the carrier immediately If the shipment is separately insured file a claim with the insurer AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO 1 3 Specifications Impedance Chip Channel dimensions 15 x 15 um 30 x 30 um 50 x 50 um 120 x 120 um 250 x 250 um or other types on request depending on application Impedance measurement Frequency range 100 kHz i 30 MHz Frequency selection Up to 4 different frequencies simultaneously Analysis range Sample volume 501 2000 ul Concentration range 1x 10 to 1 x 10 cells ml Particle size 1 60 um up to 150 um with 250 um chip Fluidics Sample flow rate 51 2500 ul min dependent on chip Pump Peristaltic pump with disposable pump head Labware compatibility 5 ml polystyrene round bottom tubes Falcon 352058 Sarstedt 55 1579 Operating System Windows 7 or 8 AmphasSoft data in fcs3 format available Software Dimensions 255 x 275 x 353 mm W x Dx H Weight 8 4 kg Operating Environment
20. osal procedures 2 3 Chemicals WARNING HAZARDOUS CHEMICALS Some chemicals used with this instrument may be hazardous or may become hazardous after completion of the protocol run e g system cleaning solution Always wear safety glasses gloves and a lab coat The responsible body e g laboratory manager must take the necessary precautions to ensure that the surrounding work place is safe and that the instrument operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Materials Safety Data Sheets MSDS or other regulatory documents Disposal of wastes must be in accordance with all national state and local health and safety regulations and laws AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO 3 Setting up Ampha Z30 3 1 Installation The instrument and the associated laptop PC are normally installed by Amphasys including some general instructions and training 3 1 1 Computer Installation and operation of the instrument Ampha Z30 requires a separate laptop PC which is usually configured and supplied by Amphasys The PC should be connected with the supplied power cord to an AC power outlet 110 240V AG 50 60Hz 3 1 2 Software and Dongle The instrument is operated with the software AmphaSoft which is preinstalled with Windows on the PC Update ve
21. oubleshooting The tables Tab 6 and 7 contain the basic troubleshooting guidelines If problems persist after performing troubleshooting contact Amphasys Technical Support Explanations and additional troubleshooting using the Expert Window in AmphaSoft are marked with EW Expert Window is explained in detail in AmphaSoft User Guide area starting General AmphasSoft software Restart Error during any process during measurement during fluid process No water No liquid after sample loading Signal during measurement Signal Chip Problem Problem the AmphaSoft program window does not appear OR appears only for some seconds Closing of AmphaSoft and immediately restart of the software error message AmphaSoft doesn react no response any running process stops and does not progress Measurement works only with low processing high rejection rate or warnings error messages of USB in log window fluid process does not progress AmphaSoft programme does not respond fluid process stops abruptly No water is running during Rinsing in the sample tube or to waste no flow detected no signals are collected signal collection stops after some time of measurement If the problem with the Chip cannot be solved with any of the suggestions put in another chip and try measurement Cause Instrument not switched on or not connected to the PG Turn instrument on or connect USB cable and restart
22. rmation how to use or measure with AmphasSoft are explained in the AmphaSoft SW User Guide AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com User Manual Ampha ZOO Workspace View Tools Help ODS MIE E Expert Window Tubing A gt Large Settings Instrument Cleaning gt Medium Sample Small id EEA Name id A00000 E Freq 0 5 MHz 1 Accepted Concentration cells ml R d 36 ejecte Start Measurement Start Rinsing Ready Figure 5 AmphaSoft Main GUI e Sample preparation Make a single cell suspension in the correct buffer and filter the sample with an appropriate filter Cell dilution is dependent on chip channel diameter Tab 4 Chip channel diameter HE omme cell Filter pore size concentration 15 x 15 um 11 40 Mio per ml 5 or 10 um 30 x 30 um 0 5110 Mio per ml 20 um 30 um 50 x 50 um 0 251 4 Mio per ml 301 40 um 120 x 120 um 0 01 1 0 3 Mio per ml 50 70 um 100 um 250 x 250 um 5000 i 50000 per ml 150 um 200 um Table 4 Available AmphaChips recommended cell concentrations for measurement and prefiltering AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com TE User Manual Ampha Z30 4 Maintenance The instrument cleaning cycle should be performed as a part of daily measurement routine
23. rsions will be provided by Amphasys or can be downloaded from Amphasys website in the download area The use of AmphaSoft needs a license which is provided with a USB dongle This dongle has always to be plugged in the PC or laptop to be used with the software or instrument Further details are described in the AmphaSoft user guide IM 7 3 1 3 Instrument The instrument must be connected to an AC power outlet 110 240V AC 50 60Hz The power lines to the equipment should be voltage regulated and surge protected Connect the instrument with the provided mini USB cable to a USB port of the PC Fig 2 To protect operating personnel the National Electrical Manufacturers Association NEMA recommends that the instrument is grounded correctly The instrument is equipped with a 3 conductor AC power cord When connected to an appropriate AC power outlet ground the instrument To preserve this protection feature do not operate the instrument from an AC power outlet that has no grounding connection 3 1 4 AmphaChips The AmphaChips listed in table 2 are the actual available chips from Amphasys In the table 2 is also listed with which tubing set the chips can be applied and the recommended pump speeds for running Recommended particle concentration and sample filtration is given in table 3 section 3 22 Chip Chip channel Measurement A00000 15 x 15 um 11 50 rom 101 20rpm EJ 30 x 30 um br 17 300 rom 30 i 50 um C00000 50 x 50 um NK 11 30
24. ructions E2 E3 E4 AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com 18 Solution What to do Success User Manual Ampha ZOO If yes go to If no go to 3 1 3 2 4 1 4 2 1 Cell sedimentation 2 1 2 2 1 Bad electronic connections 1 1 1 2 1 3 clockwise right button put pump speed to 100 rom Unscrew fitting from tubing set to the chip left inner port insert sample tube with water uncheck valves put pump speed to 100rpm or check right valve to use with system water take out chip unscrew a fitting with tubing from the cleaning device screw it in the port to the chip connect syringe to this tubing and try to push water Unscrew fitting from tubing set to the chip left inner port insert tube with water uncheck valves put pump speed to 100rpm Check tubing set for blocking gaps etc Unscrew all fittings from tubing set rinse carefully with syringe from cleaning device from all fittings try to measure cells at higher speed EW adjust pump speed sedimentation will appear further use high concentrated samples and short measurement times stay with higher pump speed dissolve sample in more viscous liquid prepare a solution with Ficoll400 10 15 in buffer this slows down the fluidics adjust pump speed in EW rinse well afterwards take out chip try the followin
25. snnanennnnennsrnnnnennnennnsennnsennnene 17 5 2 Limited VE EE 20 PONNI 20 5 4 Technical SUD DOW GA 20 STEN NNN 20 AMPHASYS AG TECHNOPARK Lucerne Platz 4 CH 6039 Root D4 Switzerland phone 41 41 541 91 20 info amphasys com www amphasys com Ke User Manual Ampha Z30 1 Introduction Ampha Z30 takes advantage of the superior sensitivity of its microfluidic chip for measuring the electrical properties of any kind of cells yeasts plant pollen bacteria animal and human cells This technology does not need the use of specific biomarkers thus most analyses can start right after sampling The device can measure changes in cell size membrane capacitance and cytoplasmic conductivity parameters whose alterations characterise many cellular processes It is best suited for routine pre diagnostic and quality control analyses In addition it covers many classical research applications as for example apoptosis cell differentiation and ploidy analyses 1 1 About the User Manual This User Manual provides detailed information about the Ampha Z30 impedance flow cytometer setup requirements specifications and maintenance including troubleshooting help and service information Information about AmphaSoft is supplied in the separate AmphaSoft SW User Guide Further details accessories chips etc you can find at www amphasys com community rg Ampha 239 i ea Figure 1 The Ampha Z30 Impedance Flow Cyto
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