User Manual - Labo America, Inc.
Contents
1. Adjust the aperture iris diaphragm Epi Illuminator Ref Fig on Pg 7 Engage the objective to be used in the light path and bring the specimen in focus Engage the required filters Adjust the brightness Observe Specimen 1 Click the main switch tol ON as shown in Figure 17 2 Rotating the light intensity adjustment knob the direction of the arrow increases the brightness and rotating the knob in the opposite direction decreases the brightness The intensity bar on the knob indicates the direction of intensity level Fig 13 Placing specimen on the stage Place the Metallurgical specimen on the stage plate The specimen should have sufficient polished reflective surface In case your microscope has mechanical stage mounted use appropriate holder plates See details on Page 13 Adjusting the Focus Focusing Procedure use of Coarse focus knob amp Fine Focus Knob 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We recommend starting with 10X 2 While observing the specimen through the eyepieces slowly rotate the coarse adjustment knob 1 counterclockwise to lower the objectives 3 When coarse focusing of the specimen is obtained an image is observed rotate the fine adjustment knob 2 for fine de2. potential electrical hazards when replacing halogen bulb turn the microscopes main switch to the OFF position and disconnect power cord from wall outlet in advance Whenever you replace your microscope bulb allow lamp socket and bulb to cool before touching Fig 1 Applicable bulb replacement 6V30W Halogen bulb P N EL 455 Safety Symbols The following code categories describe the degree of danger or damage likely to be incurred in the event of user error made in ignorance of these symbols Symbol Explanation Hot parts symbol A This symbol is placed on bulb housing and on other parts to indicate that they may be hot Never touch those parts just after the equipment has been used Allow sufficient time to cool before touching them In the event of user error it is possible to injury In the event of user error death or serious injury is possible WARNING attention In the event of user error product may be damaged The following describes icon directives This icon denotes Refer to User Manual and Caution This icon represents the productive earth This icon represents alternating current This icon represents the power switch is turned OFF This icon represents the power switch is turned ON _ Labeling Infromation SN Number following this symbol indicate the serial number of the equipment REF Character following the symbol indica
3. A LABOMED MET 400 User Manual Inverted Metallurgical Microscopy To ensure proper use of this instrument as well as to instrument understanding this manual completely before use is highly recommended avoid injury while operating CONTENTS INTRODUCTION The MET 400 inverted microscope sets a new standard for inverted metallurgical microscopes It combines high performance and distinctive styling with the high quality craftsmanship for which Labomed is known The excellentimage and comfort of use are the basics of Labomed MET400 The ergonomics of the design an aesthetically configured MET400 is the instant choice for Metallurgical Studies amp relative sciences Offered with Infinity Corrected True color optical system choices of observation tubes soft feel turret movement stage with micro manipulation offer a paramount performance The user should be aware that the protection provided by the equipment may be impaired if used with accessories not provided or recommended by the manufacturer or used a manner not specified by the manufacturer PA lt SAFETY INFORMATION 1 Itis important to follow Safety Instructions carefully to avoid any kind of personal injury or damage to this Instrument It is recomended for your safety to read the manual throughly amp do not discard this manual rather preserve it for easy reference while operating for best results 2 To avoid
4. a soft brush or compressed air cans available at your local electronics store For removing finger marks or grease soft cleaning cloth or lens tissue lightly moistened with cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use Methanol Observe sufficient caution in handling Methanol Place the objectives and or eyepieces on a dust free surface e g aluminum foil All other optical components to be cleaned should be as accessible as possible 3 Blow all loose dust particles away with compressed air or mini dust blower Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab 5 To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfactory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol Grease must always be removed using a solvent Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Clean
5. age 7 Adjusting the Interpupillary Distance IPD Fig 7 Adjusting the Diopter Fig 9 The inter pupillary distance adjustment consists of regulating the two eyepieces to align with both the eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces laterally until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it can be quickly referred to in the future This is especially important if multiple users work with the microscope Adjust the Diopter setting to 0 position by matching the fiducial lines on both eyepieces Now looking through one eye piece either left or right turn the coarse and fine focus adjustment knobs to bring the specimen into focus Adjust the focus of other side of eye piece by looking through it and at the same time optimizing the Dioptre scale of the Eye Piece Using the Eye Guards When Wearing Eyeglasses Use with the eye guards in the normal folded down position This will prevent the eyeglasses from being scratched Fig 9 Part 1 When Not Wearing Eyeglasses Extend the folded eye guards outwards direction of the arrow to prevent ambie
6. ing Solution as explained in point 3 above zig zag motion X spiral motion Wipe using a spiral movement Do not use a zig zag motion 2 Cleaning of painted surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth Loose dust and other dirt particles can be removed using a soft bristle brush used exclusively for this purpose _ 12 1 Illumination Built in Incident illumination system Halogen Lamp 6V 30W 2 Focusing mechanism turn Stage height adjustment mechanism Fine adjustment scale 0 002mm per graduation Fine adjustment stroke 0 2mm per Total stroke 20mm Co axial coarse and fine focusing on ball drive 3 Revolving nosepiece Quadruple positions fixed 4 Observation tube Binocular Trinocular Ergonomic Field number 22 22 22 Tube tilting angle 30 30 25 Interpupillary distance 52 75 52 75 52 75 adjustment range 5 Stage Size 240 x 160mm Movement range 78 x 54mm with Mechanical Stage Specimen holder 3 Different Shapes 7 Dimensions amp Weight 495 0mm L x 300 0mm W x 470mm H 9 5 kg net 8 Electrical Lamp Halogen 6V 30W Lamp life up to 100 hours Input 100V 240V AC 50 60 Hz 9 Operating environment Indoor use Altitude Max 2000 meters Ambien
7. lopment Labo America Inc reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 991 9862 CA 94538 sales laboamerica com LABOMED and TCM 400 are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice 2009 Labo America Inc 7125000 990A 12 2009 Printed in U S A
8. nt light from entering into your line of vision Fig 9 Part 2 4 Mounting the Mechanical Stage The Mechanical stage is to be mounted on the Right Side of Square a Stage with the help of two clamping screws provided underneath of the plain square stage shown with arrow marks on Fig 10 1 Fig 10 1 Mounting Holes Ref 7121300 Fig 10 2 Bulb Replacement Make sure the Power Cord is not connected Use only recommended 6V 30W high intensity Halogen bulb Applicable bulb replacement 6V 30W Halogen Bulb P No Ref EL 455 Pull out the lamp socket and pull out the blown bulb using a soft cloth Gently insert the new bulb Do not squeeze or press the bulb too much as it can cause damage to the bulb and hand Fit the lamp socket by aligning the guide pins with the condenser guide holes Push the lamp socket gently into the transmitted light illuminator Fig 11 12 Fig 12 8 Click the main switch to ON Place the specimen on the stage Polished surface facing the objectives below On Off Engage the 10X objective in the light path Adjust the observation tube and eyepieces Adjust the interpupillary distance Adjust the dioptric setting
9. t or dust is visible in the field of view Dirt dust on the specimen Dirt dust on the eyepieces Clean the specimen Clean them thoroughly 3 Visibility is poor Image is not sharp Contrast is not sharp Details are indistinct The specimen is not correctly engaged The aperture iris diaphragm is opened or stopped down too far in brightfield observation Replace it with a clean specimen Clean eyepieces gently Adjust the aperture 4 One side of image is blurred The revolving nosepiece is not correctly engaged The specimen is not correctly mounted on the stage Make sure that the revolving nosepiece clicks properly into place Place it correctly on the stage 5 The bulb flickers and the brightness is unstable 6 The coarse adjustment knob is too difficult to rotate The line voltage fluctuates The bulb is nearly burned out The power cord is not connecting securely The tension adjustment ring is tightened too much The tension adjustment ring is loosened too much Use a voltage stabilizer Replace the bulb Connect it securely Adjust it appropriately Tighten it appropriately 7 The field of view of one eye does not match that of the other Incorrect interpupilary distance adjustment Incorrect diopter adjustment Adjust the interpupilary distance Adjust the diopter www laboamerica com Our policy is one of continuous deve
10. t temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humidity at 40 C 104 F Supply voltage 100 VAC TO 240 VAC Supply voltage fluctuations Not to exceed 10 of the normal voltage Power consumption 50W Fuse F5A 250V Power supply to the halogen lamp 6V AT 30W Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 CAUTION HIGH VOLTAGE FUSE REPLACEMENT WARNING HIGH VOLTAGE POWER INLET EARTH WARNING HIGH TEMPERATURE gt 1 2 TROUBLESHOOTING GUIDE Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please review the following list and take remedial action as needed In case of any other problem which cannot be rectified as below please contact the service agents Trouble Cause Remedy 1 Although the Power is on the field of view is dark The socket pin is not connected from the illumination column to the back of the instrument The bulb is burned out The light intensity control is set to too low The objective is not in position Fuse is blown Connect it securely Replace the bulb Set the illumination to desired position Make sure the objective is clicked properly Replace fuses 250V 5 Amps 2 Dir
11. tail focusing 4 Adjusting Torque for Coarse Focus Knob Rotation of tension adjusting ring towards coarse knob will increase focusing torque Rotating tension adjusting ring towards stand will decrease the focusing torque Y Working Distance WD The WD refers to the distance between each objective and the specimen when acute focus of the specimen is obtained Objective Magnification 5X 10X 20X 50X WD mm 17 5 6 8 11 1 8 2 __ __ 10 Dia 25mm Dia 38mm Dia 53mm Installation and Operation of Optional Accessories iVu Camera Module System 1 Mount the Video adapter Ref t 3143300 712 on Trinocular observation head 2 MountiVu Camera Module System on video adapter Side View Back View Fig 15 Care amp Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean Always cover the microscope with the provided dust cover when not in use 1 Optical Cleaning 1 The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage 2 To clean the lens surfaces remove dust using
12. te the catalog number of the equipment oo 2 UNPACKING YOUR MICROSCOPE MET400 comes securely packed in the custom configured foam packing to prevent it from all shocks and transit damages After removing your Microscope from its packing make sure that all of the following contents are present Please note that the contents of your Microscope may vary as the optional configuration contrasting method or viewing body opted for may not be of the standard configuration highlighted here 1 Microscope body 2 Observation tubes as ordered 3 Eyepieces Pair 4 2 Filter Sliders ND amp NCB 5 Stage Plate fitted on Body 6 Objectives Set Standard 5x 10x 20x amp 50x 7 Epi Illuminator assembly with Two Filter Slots 8 Installation tools Allen Key 3mm 9 Power Cord 10 Mechanical Stage Optional if ordered 11 Specimen Holder Plate 12 Three different size of Petri Dish holders 13 Two Hand Rests DIAGRAM SHOWING PARTS Eyepieces _ Observation Head Binocular Screw For Fixing Binocular head eer Camera Port Specimen Holder Mechanical Stage Plate Optional Revolving Turret For Objectives Light Intensity Control knob Control Knobs For Coarse amp Fine Focusing oo ON OFF Switch TRINOCULAR MET 400 Fig 2 L N K E 225 Lamp House Co
13. ure lever your required image quality dS Observation Head Fig 4 Mounting the Objectives Fig 6 Base unit is assembled with co axial focus mechanism turret and stage plate as per procedure below Install the observation head using the following procedure as shown in Fig 4 1 Using the 3mm allen wrench provided loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on the observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity provided in the microscopes arm 3 Tighten the Head Locking Screw 1 after positioning the Observation Head as desired See figure 4 Settle down the nosepiece by rotating coarse adjustment knob towards back Remove the dust prevention caps from the nosepiece Screw the objective with the lowest magnification into the revolving nosepiece from the left side of the microscope Turn the nosepiece clockwise and mount the remaining objectives in ascending magnification order Fig 5 Clean the objectives periodically A Be sure to cover any unused threaded position on Turret with the dust prevention caps to prevent from dirt and dust from getting inside Insert the eyepieces into the ocular tube of Observation Head after removing the protective caps from the observation tube For Dioptre Adjustment on Eye Piece See P
14. ver Coaxial Control Knobs For Stage amp Y Movement EPI Illuminator Lamp Holder Removable For Bulb replacement 4 Illuminator The Illuminator Unit is to be fixed to the main Microscope Body with 3 screws using Allen wrench supplied along with Illuminator Three Tapped Holes are provided in the Base Unit as shown in Diagram below Important Note Make sure the Power Cord is not connected at this stage After the Illuminator Assembly is properly fixed connect the cable from Lamp House to the Socket provided in Base of Microscope as shown in below picture Aperture Diaphragm lever EPI Illuinator Cable connecting to above Fig 3 Adjusting the Aperture Diaphragm In order to achieve good resolution of the optical image the contrast and depth of focus it is necessary to control the numerical aperture ofthe Illumination system through the lever provided on the illuminator See Diagram above Reducing the aperture increases the contrast amp the depth of focus Increasing the aperture increases the brightness amp resolution 1 Place a specimen with plain surface on the stage 2 Focus on the specimen with 10x Objective 3 Remove of the Eyepiece amp look through the Eyepiece Tube to ensure the diaphragm image is visible in the pupil of the Objective 4 It is recommended to keep the Aperture Diaphragm image in nearly 3 4th of the pupil of Objective Adjust with apert
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