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Agilent 1290 Infinity II Variable Wavelength Detector User Manual

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1. Figure 16 Choosing a Flow Cell for G7114B for ultra fast separation with RRLC systems For ultra fast analysis with step gradients the micro flow cell 2 uL 3 mm gives the best performance e In high resolution analysis time is not the highest priority Higher delay volumes are accepted Therefore we recommend to use the damper plus mixer for a highest signal to noise If longer columns gt 50 mm for higher resolution are used then the next larger flow cell is the preferred choice for higher sensitivity Agilent 1290 Infinity II VWD User Manual 65 5 66 Optimizing the Detector Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance Absorbance log T log exCxd where T is the transmission defined as the quotient of the intensity of the transmitted light divided by the intensity of the incident light Ip e is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature and other parameters C is the concentration of the absorbing species mol L d m is the path length of the cell used for the measurement Therefore flow cells with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in the signal to noise ratio For example in Figure 17 on p
2. Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1290 Infinity II VWD User Manual 77 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 78 Agilent 1290 Infinity Il VWD User Manual Probable cause 1 Leak
3. Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Agilent 1290 Infinity II VWD User Manual 25 2 Site Requirements and Specifications Bench Space The detector dimensions and weight see Physical Specifications on page 27 allows you to place the detector on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inch in the rear for air circulation and electric connections If the bench should carry an Agilent 1200 Infinity Series system make sure that the bench is designed to
4. Standard Flow Cell 10 mm 14 uL on page 142 Micro Flow Cell 3 mm 2 uL on page 144 Semi micro Flow Cell 6 mm 5 uL on page 146 e High Pressure Flow Cell 10 mm 14 uL on page 148 Preparations Turn the lamp OFF 1 Open the doors 2 Disconnect the inlet capillary and outlet tubing from the flow cell 126 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Replace the Flow Cell Cuvette Holder 3 Unscrew the two thumb screws 4 Pull the flow cell out of its location 5 Insert the flow cell into its location and press it in in the center of the flow cell 9 Perform a Wavelength Verification Calibration to check the correct position of the flow cell cuvette holder Agilent 1290 Infinity II VWD User Manual 127 9 Maintenance Repairing the Flow Cells Parts required Description 1 Flow cell For details on flow cells see Standard Flow Cell 10 mm 14 uL on page 142 e Micro Flow Cell 3 mm 2 uL on page 144 Semi micro Flow Cell 6 mm 5 pL on page 146 High Pressure Flow Cell 10 mm 14 pL on page 148 The shown cell parts will differ depending upon the flow cell type For detailed parts schematics refer to above mentioned pages 128 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 1 Cell screw 2 Conical springs 3 Ring 1 PEEK Gasket 1 small hole Window Quartz Gasket 2 large ho
5. 168 Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The ERI REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shutdown prepare and so on With the appropriate software the LAN connector may be used to control the module from a computer through a LAN connection This connector is activated and can be configured with the configuration switch e With the appropriate software the USB connector may be used to control the module from a computer through a USB connection e The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1290 Infinity II VWD User Manual 169 12 Hardware Information Elec
6. ag Agilent Technologies Notices Agilent Technologies Inc 2014 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 67114 90000 Rev B Edition 12 2014 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document a
7. s internal power overhead If a connected device requires more power the firmware detects this overcurrent detection and provides the information to the user interface Fuse used for safety protection on board Short circuit will be detected through hardware USB Universal Serial Bus USB Universal Serial Bus replaces RS232 supports 178 a PC with control software for example Agilent Lab Advisor USB Flash Disk Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Setting the 6 bit Configuration Switch Setting the 6 bit Configuration Switch The 6 bit configuration switch is located at the rear of the module with FUSION electronics Switch settings provide configuration parameters for LAN and instrument specific initialization procedures All modules with FUSION electronics e Default is ALL switches DOWN best settings Default IP address for LAN 192 168 254 11 e For specific LAN modes switches 4 5 must be set as required e For boot resident cold start modes switches 1 2 or 6 must be UP Configuration switch a 22222228 a a CEE enen 295598 Figure 49 Location of Configuration switch example shows a G7114A B VWD Agilent 1290 Infinity I VWD User Manual 179 12 Hardware Information Table 15 6 bit Configuration Switch Mode Function Setting Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6 com 0 n a n a LAN Init Mode n a Use Default IP Address
8. Dark Current Test 107 Dark Current Test Failed 108 Holmium Oxide Test 109 When to do the Test 109 Interpreting the Results 110 Holmium Oxide Test Failed 111 D A Converter DAC Test 112 D A converter Test Evaluation 114 Other Lab Advisor Functions 115 EMFs Early Maintenance Feature 115 This chapter describes the detector s built in test functions ee Agilent Technologies 95 8 Test Functions Introduction Introduction All tests are described based on the Agilent Lab Advisor Software B 02 06 Other user interfaces may not provide any test or just a few For details on the use of the interface refer to the interface documentation The Lab Advisor shows the available test under Service amp Diagnostics w amp Agilent Lab Advisor f amp Tasks YF Filter Lab Advisor PA System Overview ASTM Drift and Noise Test Configure x T as FE Firmware Update WB Loos Resuts 3 G7114B Service amp Diagnostics Name ASTM Drift and Noise Test Approx Time 20min Description The test performs ASTM drift and noise evaluation Context Help F1 Hep Topics what s New Figure 21 The Lab Advisor shows the available test 96 Agilent 1290 Infinity Il VWD User Manual Test Functions 8 Intensity Test The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can
9. Figure 20 The Lab Advisor shows the available test 72 Agilent 1290 Infinity Il VWD User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status Early Maintenance Feedback counters EMF instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon request the user can send this report to Agilent for a significantly improved troubleshooting and repair process The Agilent Lab Advisor software is available in two versions e Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal
10. correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 79 7 80 Error Information Lost CAN Partner Leak Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Leaking flow ce
11. detector 37 Agilent 1290 Infinity Il VWD User Manual setting up an analysis 50 settings method parameters 41 setup ChemStation 205 shutdown 78 site requirements environment 26 power considerations 24 power cords 25 solvents 223 sound emission 222 source lens assembly 14 special interfaces 175 special settings boot main 181 boot resident 181 specification physical 27 specifications 30 noise and linearity 30 physical 27 stepper motor 16 T TCP IP parameter configuration 189 telnet configuration 199 temperature sensor 80 test functions 95 test function D A converter 112 DAC 112 introduction 96 tests available tests vs interface 72 holmium oxide 109 intensity of deuterium lamp 97 wavelength calibration 102 Index timeout 78 troubleshooting available tests vs interface 72 error messages 77 test functions 95 U USB 174 Using detector 54 priming and purging the system 50 requirements and conditions 52 running the sample 56 setting up an analysis 50 the cuvette holder 131 typical chromatogram 53 V voltage range 27 W warm up of detector 69 warnings and cautions 118 waste electrical and electronic equipment 220 wavelength calibration 102 WEEE directive 220 weight 27 Agilent 1290 Infinity Il VWD User Manual 229 www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity II Variable Wavelength Detector G7114B The manual
12. it goes through a cycle of different states e 0 to 0 5 minutes the heater control is OFF and the heater element runs at 0 duty cycle e 0 5 to 1 minutes the heater control is OFF and the heater element runs at 66 duty cycle This first minute is used as self test of the heater functionality 1 to 30 minutes the heater control is OFF and the heater element runs at 40 duty cycle e After 30 minutes the heater control is ON and is working with optimized parameters to get the optical unit into the optimal temperature window stabilized This cycle starts e when the detector is turned off on when the lamp is turned off on to ensure that the temperature control operates in a defined control range The times to stabilize the baseline may vary from instrument to instrument and depends on the environment The example below was done under stable environmental conditions The figures below show the first two hours of a detector warm up phase The lamp was turned on immediately after turn on of the detector Agilent 1290 Infinity II VWD User Manual 69 5 Optimizing the Detector Warm up of the Detector Baseline 230 4 360 100 SLW 4 RT 2s Figure 18 Detector Warm up 1 hour Baseline 230 4 360 100 SLW 4 RT 2s Board Temperature a Figure 19 Detector Warm up 2 hour 70 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity II VWD User Manual Available Tests versus Interfaces 72 e
13. 21 operating temperature 27 optical unit beam splitter assembly 16 entrance slit assembly 14 filter assembly 15 filter 15 flow cell 13 grating assembly 16 lamp 14 mirrors 16 228 photodiode assemblies 16 photodiode boards 16 source lens assembly 14 stepper motor 16 optimization detector performance 64 performance 63 overview cable 154 optical path 12 optical system 12 system overview 12 P parts for maintenance cuvette holder 150 high pressure flow cell 148 micro flow cell 144 semimicro flow cell 146 standard flow cell 142 parts maintenance 139 PC and Agilent ChemStation Setup 202 PC setup local configuration 202 performance specifications 28 performance optimization 64 photodiode assemblies 16 boards 16 photometric accuracy 67 physical specifications 27 physical specifications 27 power consumption 27 power cords 25 power considerations 24 preparing detector 47 product description 11 Q quick noise test 105 Radio Frequency Identification flow cell and lamp 10 radio interference 221 remote ERI 174 remote cables 158 remove doors 122 flow cell 126 repairs cautions and warnings 118 overview of simple repairs 120 replacing firmware 136 replace flow cell 126 RFID Radio Frequency Identification 10 RS 232C cable 163 running the sample 56 S safety class 212 safety general information 212 standards 27 symbols 216 serial number information 1 0 171 set up
14. 5 Hz Figure 10 G7114B Dual Wavelength Settings Stoptime Posttime As Pumpiinjector of Cc min min 42 Signal Wavelength Single Wavelength 190 600 nm step 1 Dual Wavelength Mode enables the multi wavelength mode with two wavelengths Peakwidth Responsetime Data Rate Peakwidth enables you to select the peak width response time for your analysis The peak width is defined as the width of a peak in minutes at half the peak height Set the peak width to the narrowest expected peak in your chromatogram The peak width sets the optimum response time for your detector The peak detector ignores any peaks that are considerably narrower or wider than the peak width setting The response time is the time between 10 and 90 of the output signal in response to an input step function When the All spectrum storage option is selected then spectra are acquired continuously depending on the setting of the peak width The time specified by the peak width is used as a factor in the acquisition of spectra The acquisition time for one spectrum is slightly less than the peak width divided by 8 which is the acquisition time Limits When you set the peak width in minutes the corresponding response time is set automatically and the appropriate data rate for signal and spectra acquisition is selected The 1290 Infinity VWD G7114B has a data rate of up to 240 Hz Stoptime Posttime The stoptime is the time where ei
15. AutoSampler ILC System Access Recess Point 7 Click on OK 206 Agilent 1290 Infinity Il VWD User Manual LAN Configuration PC and Agilent ChemStation Setup The Configuration Editor shows now the new instrument 8 If required change under Configure Path the folder locations Save the current configuration via File Save Configuration Editor _folx File Configure Utilities Help Orstrment 1 Modular 3D LC System LC System Access Recess Point 10 Exit the Configuration Editor 11 Start the Agilent ChemStation During first startup or when the system configuration has changed a notification shows up 12 The left column shows the modules that could be configured You may select the module manually from the list We use the Auto Configuration mode Click on Yes yee ZETE Series LC Qs Qe amp BirPuro Agilent 1290 Infinity II VWD User Manual 207 13 13 LAN Configuration PC and Agilent ChemStation Setup 13 Enter the IP address or the Hostname of the module with the LAN access Agilent 1100 1200 1290 Series LC Auto x 14 Click on OK The selected module is shown now in the right window with serial number In addition all other modules connected via CAN to the detector are shown as well 15 Click on OK to continue the ChemStation loading Agilent 1100 1200 1290 Series LC _ Agilent 11007120071230 Series LC Qs S 040 16421241 Pronoms Crus
16. Gasket Tr snigbineit Figure 38 Orientation of conical springs e Screw the cell screw into the flow cell and tighten the screw Repeat the procedure for the other cell side Reconnect the capillaries Perform a leak test If OK insert the flow cell an oOo Oo A Perform Wavelength Verification Calibration on page 102 to check the correct positioning of the flow cell 8 Replace the front cover Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Using the Cuvette Holder This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications a s LL J mei Vy Li A l j K When If your own standard should be used to checkout the instrument Parts required p n Description 1 G1314 60200 Cuvette Holder 1 Cuvette with the standard e g NIST certified holmium oxide sample Preparations Remove the normal flow cell Have cuvette with standard available Agilent 1290 Infinity I VWD User Manual 131 9 Maintenance 1 Locate the cuvette holder on the desk 3 Insert the cuvette with the sample into the holder The clear side of the cuvette must be visible RAK J Clear side Light path 2 Unscrew the bracket 4 Replace the bracket an
17. Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1290 Infinity II VWD User Manual 163 11 Identifying Cables USB Cables To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC Module 5188 8049 USB A F USB Mini B M OTG Module to Flash Drive Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity I VWD User Manual 12 Hardware Information Firmware Description 166 Electrical Connections 169 Rear View of the Module 170 Information on Instrument Serial Number 170 Interfaces 172 Overview Interfaces 173 ERI Enhanced Remote Interface 176 USB Universal Serial Bus 178 Setting the 6 bit Configuration Switch 179 Special Settings 181 Instrument Layout 183 Early Maintenance Feedback 184 This chapter describes the detector in more detail on hardware and electronics ete Agilent Technologies 165 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identi
18. Introduction to the Variable Wavelength Detector 1 Product Description Product Description The Agilent 1290 Infinity II Variable Wavelength Detector VWD is the most sensitive and fastest detector in its class Time programmable wavelength switching provides sensitivity and selectivity for your applications More sample information can be acquired in the dual wavelength mode Low detector noise lt 1 5 uAU and baseline drift lt 1 x 10 4 AU h facilitates precise quantification of trace levels components High productivity can be achieved with fast analysis at up to 240 Hz data rates Status indicator Flow cell Lamp housing window and air inlet Power switch Leak drain Figure 1 Overview of the Variable Wavelength Detector Agilent 1290 Infinity Il VWD User Manual 11 1 Introduction to the Variable Wavelength Detector Optical System Overview Deuterium lamp The optical system of the detector is shown in the figure below Its radiation source is a deuterium arc discharge lamp for the ultraviolet UV wavelength range from 190 to 600 nm The light beam from the deuterium lamp passes through a lens a filter assembly an entrance slit a spherical mirror M1 a grating a second spherical mirror M2 a beam splitter and finally through a flow cell to the sample diode The beam through the flow cell is absorbed depending on the solutions in the cell in which UV absorption takes place and the intens
19. Oe Troubleshooting and Diagnostics Agilent Lab Advisor Software 73 Overview about the troubleshooting and diagnostic features ot Agilent Technologies 71 6 Troubleshooting and Diagnostics Available Tests versus Interfaces Available Tests versus Interfaces NOTE Depending on the used interface the available tests and the screens reports may vary Preferred tool should be the Agilent Lab Advisor see Agilent Lab Advisor Software on page 73 Agilent Lab Advisor B 02 06 or later is required The Instant Pilot does not support the G7114B at introduction If running as G1314E F VWD emulation mode the Instant Pilot firmware must be B 02 16 e Preferred tool should be the Agilent Lab Advisor software see Agilent Lab Advisor Software on page 73 Screenshots used within these procedures are based on the Agilent Lab Advisor software w amp Agilent Lab Advisor Service amp Diagnostics BESS YF Filter Lab Advisor PB System Overview Configuration Peps emake ASTM Drift and Noise Test Serial Fimware Update a Logs amp Results 3 G7114B A Service amp Diagnostics KO Instrument Control Q a _ System Report Name ASTM Drift and Noise Test Approx Time 20min Hell Description The test performs ASTM drift and noise evaluation elp Context Help F1 2 Help Topics whats New
20. The semi micro 1 and 2 gaskets items 6 and 7 look very similar Do not mix them up Item p n Description G1314 60183 Semi micro flow cell 6 mm 5 pL with RFID tag 5021 1823 Capillary column detector SST 400 mm lg 0 12 mm i d 1 G1314 20047 Cell screw G1314 65056 Semi micro cell kit includes two quartz windows one gasket 1 one 2 and two PTFE gaskets 2 79853 29100 Conical spring kit 10 pk 3 79853 22500 Ring SST 2 pk 4 79853 68743 PTFE gasket round hole i d 2 5 mm o d 8 mm 10 pk 5 79853 68742 Window quartz kit 2 pk 6 Semi micro 1 gasket long hole 1 5 x 3 5 mm PTFE 7 Semi micro 2 gasket long hole 2 x 4 mm PTFE 8 G1314 44010 Clip for RFI ID tag 9 0515 4780 Screw for Clip M2 2 4 5 mm long 146 Agilent 1290 Infinity Il VWD User Manual Parts and Materials for Maintenance 10 Semi micro Flow Cell 6 mm 5 pL Cell screw 2 Conical springs 3 Ring SST 4 PTFE gasket 5 Quartz window 6 Semi micro gasket 1 7 Semi micro gasket 2 8 Clip for RFI ID tag 9 Screw for clip Figure 43 Semi micro Flow Cell Agilent 1290 Infinity I VWD User Manual 147 10 Parts and Materials for Maintenance High Pressure Flow Cell 10 mm 14 pL Item p n Description G1314 60182 High pressure flow cell 10 mm 14 uL 400 bar with RFID tag G1315 87311 Capillary ST 0 17 mm x 380 mm S S 1 G1314 20047 Cell screw G1314 65054 Cell kit Agilent comprises two windows two KAPTON gaskets and t
21. a Monitoring and Control Instrumentation product Do not dispose of in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 220 Agilent 1290 Infinity Il VWD User Manual Appendix 14 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1290 Infinity II VWD User Manual 221 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 222 Agilent 1290 Infinity Il VWD User Manual Appendix 14 Solvent Information Flow Cell To protect optimal functionality of your flow cell e Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Use of
22. allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 Defective sensor or main board Please contact your Agilent service representative 2 Detector is exposed to illegal ambient Verify that the ambient conditions are within conditions the allowed range 92 Agilent 1290 Infinity Il VWD User Manual Error Information 7 Illegal Temperature Value from Sensor at Air Inlet Error ID 1072 This temperature sensor delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 The temperature sensor is defect e Replace the cable to the main board e Please contact your Agilent service representative 2 Detector is exposed to illegal ambient Verify that the ambient conditions are within conditions the allowed range Heater at fan assembly failed Error ID 1073 Every time the deuterium lamp or the tungsten lamp DAD only is switched on or off a heater self test is performed If the test fails an error event is created As a result the temperature control is switched off Probable cause Suggested actions 1 Defective connector or cable Please contact your Agilent service representative 2 Defective heater Please contact your Agilent service represe
23. and lamps with RFID radio frequency identification tags Agilent 1290 Infinity II VWD User Manual 29 2 Site Requirements and Specifications Specification Conditions G7114B ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Standard flow cell path length 10 mm flow 1 mL min LC grade methanol Noise 1 5 10 AU at 230 nm TC 2 s RT 2 2 TC Linearity Linearity is measured with caffeine at 265 nm The specification are based on the the standard RFID tag lamp G1314 60101 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit gt one hour ASTM measurements require that the detector should be turned on at least 24 hours before start of testing 30 Agilent 1290 Infinity II VWD User Manual This chapter explains the essential operational parameters of
24. be used to determine the performance of the lamp and to check for dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between instruments The figure below shows a typical intensity test spectrum The Intensity Test is available in Agilent Lab Advisor preferred tool Agilent 1290 Infinity II VWD User Manual 97 8 Test Functions Intencity INTENSITY Tes Intensity Test Evaluation The Agilent Lab Advisor and the Instant Pilot evaluate three values automatically and display the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value 1 Run the Intensity Test with Agilent Lab Advisor for further information see Online Help of user interface G7114B PPBAU00056 Intensity Test General Limits Signals Test Name Intensity Test Description The test scans the Intensity spectrum generated by the UV Lamp Module G7114B PPBAUO0056 1290 VWD Status Passed Start Time 9 8 2014 1 20 47 PM Stop Time 9 8 2014 1 21 16 PM M Test Procedure Result Name Y 1 Check Prerequisites Accumulated UV Lamp Bum Time 73 31 h W 2 Perform I
25. bear the weight of all modules The detector should be operated in a horizontal position Environment CAUTION Your detector will work within specifications at ambient temperatures and relative humidity as described in Physical Specifications on page 27 Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 26 Agilent 1290 Infinity Il VWD User Manual Physical Specifications Site Requirements and Specifications 2 Table 2 Physical Specifications Type Specification Comments Weight 11 kg 24 3 Ibs Dimensions 140 x 396 x 436 mm height x width x depth 5 5 x 15 6 x 17 2 inches Line voltage 100 240 V 10 Wide ranging capability Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC EN CSA UL 50
26. cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Description USB A M USB Mini B 3 m PC Module USB A F USB Mini B M OTG Module to Flash Drive Agilent 1290 Infinity II VWD User Manual 155 11 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 35900 A D converters p n 35900 60750 35900 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog 156 Agilent 1290 Infinity Il VWD User Manual Identifying Cables 11 Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module yoo la Shield Shield Analog pa o T Ne Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog _Se 3 Red Analog S Agilent 1290 Infinity II VWD User Manual 157 11 Identifying Cables Remote Cables ERI Enhanced Remote Interface 5188 8029 ERI to general purpose p n 5188 8029 pin Enhanced Remote Classic Remote 1 101 START REQUES
27. critical for your chromatography or your environment is stable you may set the Temperature Control to off This will lower the optical unit and flow cell temperature by some degree UV Lamp Tag Automatic detects a lamp with RFID tag If no RFID tag lamp is used UV lamp not ready is displayed and it cannot be ignited A compatible mode has to be selected based on the used lamp see Non RFID tag lamp information below Manual by PN uses the selected heating mode This mode can also be used when the RFID tag of the standard lamp Deuterium lamp with RFID tag G1314 60101 is not recognized defect RFID tag or reader Non RFID tag lamp In case a non RFID tag lamp is used the user interface will show this when selecting a compatible mode You may operate the detector outside of the guaranteed specification The correct selection is important for optimal performance and lifetime Cell Tag Automatic mode for Agilent flow cells with RFID tags If no RFID tag cell is used detector icon will become gray cell tag not ready and analysis is disabled At Power On automatic lamp on at power on Automatic Turn On automatic detector power on Agilent 1290 Infinity II VWD User Manual Using the Module 3 Method Parameter Settings Method Parameter Settings These settings are available via Menu gt Instrument gt Set up Instrument Method or via right click into the module s active area does not show the Ins
28. detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Remote Timeout Error ID 0070 Error Information 7 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Agilent 1290 Infinity II VWD User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up
29. detector GUI there are active areas If you move i VWD E the mouse cursor across the icons the cursor will change Lamp turn on and off of UV lamp EMF status Detector status Lamp status on off and information RFID tag Flow Cell information RFID tag orFrwh RFID tag information is displayed when moving with the mouse cursor on to the tag attached to the flow cell or lamp The information provides flow cell and lamp related information like Part number Production date Serial number and other details Lamp tag information Burn time 43 50 h Minimum lifetime 2000 h Number ofignitions 44 Product Number 2140 0820 Serial Number 647005 Production Date Thursday July 17 2008 Tested Date Friday October 24 2008 Intensity at test 18463 counts 38 Agilent 1290 Infinity Il VWD User Manual Using the Module 3 Jetector User Interface EMF Status shows Run Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Maintenance might be required yellow EMF warning Maintenance required red Important The EMF settings can be accessed via Agilent Lab Advisor The limit s can be changed Based on the limit the User Interface displays the above status Module Status shows Run Ready Error state and Not Ready text or Error text Error red Not ready yellow Ready g
30. g wre BG owrne of csrnecone 208 Agilent 1290 Infinity Il VWD User Manual LAN Configurati PC and Agilent Che on 13 16 You may see the details of the module by selecting the module and clicking on Configure 1100 1200 1290 DAD Configuration Instrument f Device name Type ID Serial number PROO100015 Firmware revision 8 05 23 0003 Connection settings Under Connection Settings you may change the IP Hostname of the module may require a re start of the ChemStation After successful load of the ChemStation you should see the module s as active item in the graphical user interface GUI Agilent 1290 Infinity II VWD User Manual 209 13 LAN Configuration PC and Agilent ChemStation Setup nil D NotReady Instrument 0 00 0 00 Not Ready 210 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity I VWD User Manual 14 Appendix General Safety Information 212 General Safety Information 212 Safety Standards 212 General 212 Before Applying Power 213 Ground the Instrument 213 Do Not Operate in an Explosive Atmosphere 214 Do Not Remove the Instrument Cover 214 Do Not Modify the Instrument 214 InCase of Damage 214 Solvents 215 Safety Symbols 216 Refrigerant 218 Waste Electrical and Electronic Equipment Directive 220 Radio Interference 221 Sound Emission 222 Solvent Information 223 Declaration of Conformity for HOX2 Filter 224 Agilent Technolog
31. or 60 Hz 5 80 VA 70 W 4 55 C 39 131 F 40 70 C 40 158 F lt 95 r h at 40 C 104 F Non condensing Up to 2000 m 6562 ft Up to 4600 m 15092 ft For storing the module Installation category II Pollution degree2 For indoor use only Agilent 1290 Infinity II VWD User Manual 27 2 Site Requirements and Specifications Performance Specifications G7114B Performance Specifications Table3 Agilent 1290 Infinity II Variable Wavelength Detector G7114B Performance Specifications Feature Specification Detection type Double beam photometer Light source Number of signals Maximum data rate Noise Drift Linearity Wavelength range Wavelength accuracy Wavelength precision Slit width Time programmable Deuterium lamp Single and dual wavelength detection 240 Hz single wavelength detection 2 5 Hz dual wavelength detection lt 0 15 10 AU at 230 nm single wavelength detection lt 0 80 10 AU at 230 nm and 254 nm dual wavelength detection lt 1 10 AU h at 230 nm gt 2 5 AU upper limit 190 600 nm 1 nm self calibration with deuterium lines verification with holmium oxide filter lt 0 1 nm 6 5 nm typical over whole wavelength range Wavelength polarity peak width lamp on off Agilent 1290 Infinity Il VWD User Manual Site Requirements and Specifications 2 Table 3 Agilent 1290 Infinity II Variable Wavelength Detector G7114B Pe
32. the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Agilent 1290 Infinity I VWD User Manual 167 12 Hardware Information Main FW update Resident System Main System Resident FW Update Figure 46 Firmware Update Mechanism Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761
33. the lamp Then a check of the deuterium emission line 656 nm via the reference diode is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable cause Suggested actions 1 Calibration incorrect Recalibrate the detector Cutoff filter doesn t decrease the light intensity at 250 nm Error ID 7813 The automatic filter check after lamp ignition has failed When the lamp is switched on the detector moves the cutoff filter into the light path If the filter is functioning correctly a decrease in lamp intensity is seen If the expected intensity decrease is not detected the error message is generated Probable cause Suggested actions 1 Motor is not connected Please contact your Agilent service representative 2 Defective motor Please contact your Agilent service representative 3 Defective missing grating or filter Please contact your Agilent service representative 4 Cable connector defective Please contact your Agilent service representative Agilent 1290 Infinity II VWD User Manual 91 7 Error Information ADC Hardware Error Error ID 7830 7831 A D Converter hardware is defective Probable cause Suggested actions 1 A D Converter hardware is defective Please contact your Agilent service representative Illegal Temperature Value from Sensor on Main Board Error ID 1071 This temperature sensor located on the detector main board delivered a value outside the
34. the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1290 Infinity II VWD User Manual 183 12 Hardware Information Early Maintenance Feedback 184 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum
35. x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 28 56 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 5 Use the and press Enter to list the current settings 36D317521C sing Stored gt no connections Telnet Current settings in Using Stored mode information about the LAN interface MAC address initialization mode Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to PC with controller software e g Agilent ChemStation here not connected Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 6 Change the IP address in this example 192 168 254 12 and type to list current settings TT Telnet 134 40 30 205 LAN Status Page MAC Address Init Mode TCP IP activ IP P IP Status Controllers no connections Figure 64 Telnet Change IP settings change of IP setting to Initialization mode is Using Stored active TCP IP settings stored TCP IP settings in non volatile memory connected to PC with controller software e g Agilent ChemStation here not connected 7 When you have finished typing the configuration parameters type exit and p
36. your Agilent service representative 87 Error Information Wavelength calibration setting failed Error ID 7310 The intensity maximum was not found during wavelength calibration Calibration 0 Failed Calibration 1 Failed Probable cause 1 Lamp is OFF 2 Incorrect flow cell installation 3 Flow cell contamination or air bubbles 4 Intensity too low 5 Current step value too far from maximum 6 Misaligned defective grating assembly 7 Defective main board Zero order calibration failed 656 nm calibration failed Suggested actions Switch on the lamp Ensure the flow cell is installed correctly Clean replace flow cell windows or remove air bubbles Replace lamp e Repeat the calibration e Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity II VWD User Manual Error Information 7 Wavelength holmium check failed Error ID 7318 The holmium oxide test in the detector has failed During the holmium test the detector moves the holmium filter into the light path and compares the measured absorbance maxima of the holmium oxide filter with expected maxima If the measured maxima are outside the limits the error message is generated Probable cause Suggested actions 1 Misaligned defective grating assembly Ensure the flow cell is inserted correctly and is fr
37. 0 0 0 0 0 Use Stored IP Address 0 0 0 1 0 Use DHCP to request IP Address 0 0 1 0 0 Test 1 System n a n a n a ColdStart Boot Main System Keep Data 0 0 0 0 0 Boot Resident System Keep Data 1 0 0 0 0 Boot sa e to 0 0 0 0 1 Boot Resident System Revert to 1 0 0 0 1 Default Data en from non volatile memory not assigned Always keep these switches on position 0 off When selecting mode COM settings are stored to non volatile memory When selecting mode TEST COM settings are tak 3 Default IP Address is 192 168 254 11 4 Host Name will be the MAC address 180 Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Special Settings CAUTION Boot Resident Main Firmware update procedures may require this mode in case of firmware loading errors main resident firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident main mode In resident mode it is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings e Boot Main System Revert to Default Data The instrument will boot to main mode and changes to the module s default parameter May be also req
38. 1290 Infinity II VWD User Manual 185 12 Hardware Information Early Maintenance Feedback 186 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity I VWD User Manual 13 LAN Configuration What You Have to Do First 188 TCP IP parameter configuration 189 Configuration Switch 190 Initialization Mode Selection 191 Dynamic Host Configuration Protocol DHCP 195 General Information DHCP 195 Setup DHCP 196 Manual Configuration 198 With Telnet 199 PC and Agilent ChemStation Setup 202 PC Setup for Local Configuration 202 Agilent ChemStation Setup 205 This chapter provides information on connecting the module to the Agilent ChemStation PC Ee Agilent Technologies 187 13 LAN Configuration What You Have to Do First The module has an on board LAN communication interface NOTE This chapter is generic and may show figures that differ from your module The DUAA functionality is the same 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module underneath the configuration switch see Figure 51 on page 188 G4212 65800 aig vee Part number of the detector main board B 01 XX 0925 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Figure
39. 50 MAC Label Country of Origin 2 Connect the instrument s LAN interface see Figure 51 on page 188 to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable LAN interface MAC label Figure 51 Location of LAN interfaces and MAC label 188 Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 196 e by manually setting the parameters using Telnet The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration
40. Bootp 191 cable 162 configuration switche 190 Agilent 1290 Infinity I VWD User Manual Index manual configuration with telnet 199 manual configuration 198 PC and Agilent ChemStation Setup 202 TCP IP parameter configuration 189 using default 193 using stored 193 what you have to do first 188 188 eak sensor open 81 eak sensor short 81 eak correcting 133 ine frequency 27 ine voltage 27 ocal configuration 202 ost CAN partner 80 M MAC address 188 maintenance correcting leaks 133 definition of 118 exchanging leak handling system 135 feedback 184 introduction 117 overview 120 parts 139 replacing firmware 136 standard flow cell 128 using the cuvette holder 131 manual configuration of LAN 198 message ADC hardware error 92 calibration failed 88 calibration lost 91 filter check failed 91 227 Index grating filter motor defective 90 heater current missing 87 heater failed 93 heater power at limit 94 holmium oxide test failed 89 ignition without cover 83 83 illegal temperature value from sensor at air inlet 93 illegal value from sensor on main board 92 lamp current missing 85 lamp ignition failed 86 lamp voltage missing 86 remote timeout 79 wavelength check failed 91 mirror assemblies 16 module firmware replace 136 Noise and Linearity specifications 30 Noise Test 104 non operating altitude 27 non operating temperature 27 0 operating Altitude 27 operating principle
41. D User Manual Error Information 7 Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Open Cover Error ID 0205 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative 3 Defective sensor or main board Please contact your Agilent service representative Agilent 1290 Infinity I VWD User Manual 83 7 Error Information ERI Messages Error ID 11120 5 V 11121 25 V ERI Messages The ERI Enhanced Remote Inter
42. EMF counters for all modules in all systems Y Filter All Counters B E G7114B ___ G7114B 1290 VWD MEE Serial PPBAU00056 Number of UV Lamp Ignitions Activate EMF Deactivate EMF ii Refresh Counters Figure 36 EMFs Agilent 1290 Infinity II VWD User Manual 115 8 Test Functions Other Lab Advisor Functions 116 Agilent 1290 Infinity Il VWD User Manual This chapter provides general information on maintenance of the detector Agilent 1290 Infinity I VWD User Manual 9 Maintenance Introduction to Maintenance 118 Warnings and Cautions 118 Overview of Maintenance 120 Cleaning the Module 121 Remove and Install the Doors 122 Replace the Deuterium Lamp 123 Replace the Flow Cell Cuvette Holder 126 Repairing the Flow Cells 128 Using the Cuvette Holder 131 Correcting Leaks 133 Replacing Leak Handling System Parts 135 Replace the Module Firmware 136 Ee Agilent Technologies 117 9 Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack There are no serviceable parts inside Do not open the module Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing go
43. Probable cause Suggested actions 1 Cell contaminated Flush flow cell 2 Cell windows are contaminated Clean replace cell windows 3 Mechanical problem Check cell position Agilent 1290 Infinity II VWD User Manual 101 Test Functions Wavelength Verification Calibration 102 Wavelength calibration of the detector is done using the zero order position and 656 nm emission line position of the deuterium lamp The calibration procedure involves two steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector In addition to the zero order and 656 nm alpha emission line calibration the beta emission line at 486 nm and the three holmium lines are used for the complete wavelength calibration process These holmium lines are at 360 8 nm 418 5 nm and 536 4 nm The wavelength verification calibration takes about 2 5 min and is disabled within the first 10 min after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically The Wavelength Verification Calibration is available in Agilent Lab Advisor preferred tool When to Calibrate the Detector The detector i
44. Solvents Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt gt 2COCly 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropyl ether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1290 Infinity II VWD User Manual 223 14 Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter 224 Declaration of Conformity We herewith inform you that the Holmium Oxide Glass Filter used in Agilents absorbance detectors listed in the table below meets the requirement
45. T D Sub female 15way user s view to connector 2 102 STOP egZ9aeaRBPRB 3 103 READY C 104 POWER ON Tae Se ee 5 105 NOT USED D D O08 weeooegs 6 106 SHUT DOWN lt 0 O ou S 7 107 START 8 108 PREPARE 9 1wire DATA 10 DGND 11 5V ERI out 12 PGND 13 PGND 14 24V ERI out 15 24V ERI out 5188 8044 ERI to ERI Connector D_Subminiature 15 pin 158 Agilent 1290 Infinity II VWD User Manual Table 12 5188 8044 ERI to ERI Identifying Cables 11 p n 5188 8044 Pin ERI Signal Pin ERI o 10 GND 10 m 2 1 Start Request 1 2 Stop 2 3 Ready 3 5 Power on 5 4 Future 6 Shut Down 6 7 Start 7 8 Prepare 8 Ground Cable Shielding NC Connection 5188 8045 ERI to APG Connector D_Subminiature 15 pin ERD Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG 10 GND 1 7 1 Start Request 9 2 Stop 8 3 Ready 7 5 Power on 6 4 Future 5 6 Shut Down 4 7 Start 3 8 Prepare 2 Ground Cable Shielding NC Connection 159 Agilent 1290 Infinity II VWD User Manual 11 160 Identifying Cables One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Br
46. VWD User Manual 11 Identifying Cables Cable Overview 154 Analog Cables 156 Remote Cables 158 CAN LAN Cables 162 RS 232 Cables 163 USB Cables 164 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ott Agilent Technologies 153 11 Identifying Cables Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 01046 60105 Remote cables p n 5188 8029 5188 8044 5188 8045 5061 3378 01046 60201 CAN cables p n 5181 1516 5181 1519 154 Description Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description ERI to general purpose Remote Cable ERI ERI Remote Cable APG ERI Remote Cable to 35900 A D converter Agilent module to general purpose Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Agilent 1290 Infinity Il VWD User Manual LAN cables p n 5023 0203 5023 0202 RS 232 cables not for FUSION p n board G1530 60600 RS232 61601 5181 1561 USB cables p n 5188 8050 5188 8049 Identifying Cables 11 Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Description RS 232 cable 2 m RS 232
47. a manufactured product complies with all applicable C European Community directives The European Declaration of Conformity is available at http regulations corporate agilent com DoC search htm j Manufacturing date Power symbol indicates On Off The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position 216 Agilent 1290 Infinity Il VWD User Manual Appendix 14 Table 23 Symbols Pacemaker A Magnets could affect the functioning of pacemakers and implanted heart defibrillators A pacemaker could switch into test mode and cause illness A heart defibrillator may stop working If you wear these devices keep at least 55 mm distance to magnets Warn others who wear these devices from getting too close to magnets Magnetic field A Magnets produce a far reaching strong magnetic field They could damage TVs and laptops computer hard drives credit and ATM cards data storage media mechanical watches hearing aids and speakers Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard A Q Indicates a piercing or cutting hazard WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that cou
48. age 67 the noise increased by less than 10 but a 70 increase in signal intensity was achieved by increasing the path length from 6 mm to 10 mm When increasing the path length the cell volume usually increases in the example from 5 14 uL Typically this causes more peak dispersion As demonstrated this did not affect the resolution in the gradient separation in the example that is shown below As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 Agilent 1290 Infinity II VWD User Manual Optimizing the Detector 5 Analysis of pesticide standard g 6 mm optical path length E i0 mm optical pathlength io 2 Lil oy l F Pi A i i h J Vi M Jy n J Le ool U i ena eae D ie TAA fea Hl 18 20 30 Time min Figure 17 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the detector it is necessary to have more precise information on path lengths of the flow cells The correct response is expected response correction factor Please find below the details of the flow cells Table9 Correction factor
49. amp 3 Evaluate Data Minimum Lamp On Time 0 17h Signal Noise value at 254 nm UV 0 003 mAU Maximum Allowed Noise 0 0 02 mA Figure 28 Quick Noise Test Results Agilent 1290 Infinity Il VWD User Manual 105 106 Test Functions Quick Noise Test Absorbance mAU 110 635 110 630 110 625 110 620 Time min Figure 29 Quick Noise Test Signal Agilent 1290 Infinity II VWD User Manual Test Functions 8 Dark Current Test The dark current test measures the leakage current from the sample and reference circuits The test is used to check for defective sample or reference diodes or ADC circuits which may cause non linearity or excessive baseline noise During the test the shutter is moved into the light path Next the leakage current from both diodes is measured 1 Run the Dark Current Test with the Agilent Lab Advisor for further information see Online Help of user interface G7114B PPBAU00056 Dark Current Test General Limits Signals Test Name Dark Current Test Description The test measures the dark current from the detector optic Module G7114B PPBAU00056 1290 VWD Status Passed Start Time 9 8 2014 2 06 28 PM Stop Time 9 8 2014 2 06 56 PM _ Test Procedure Result Name Y 1 Check Prerequistes Maximum Sample Inter 41 Counts W 2 Perform Dark Curent Test Maximum Sampl 7900 Count A 3 Evaluate Data Maximum Reference Intensity Measured 24 Counts M
50. and Noise Test Results UV Signal Absorbance mAU 108 50 108 45 108 40 0 5 10 15 20 Time min Figure 27 Drift and Noise Test Signal 104 Agilent 1290 Infinity Il VWD User Manual Test Functions 8 Quick Noise Test The noise test measures the noise of the detector with HPLC grade water flowing through the flow cell at 1 mL min in one minute intervals over a total of 5 minutes The noise of the detector is calculated by using the maximum amplitude for all random variations of the detector signal of frequencies greater than one cycle per hour The noise is determined for 5 one minute intervals and is based on the accumulated peak to peak noise for the intervals At least seven data points per cycles are used in the calculation The cycles in the noise determination are not overlapping In order to obtain reliable results the lamp should be turned on for at least 10 minutes prior to measurement 1 Run the Quick Noise Test with Agilent Lab Advisor for further information see Online Help of user interface Quick Noise Test G7114B PPBAU00056 General Limits Signals Test Name Quick Noise Test Description The test performs a quick noise evaluation Module G7114B PPBAUO00056 1290 VWD Status Passed Start Time 9 8 2014 1 34 27 PM Stop Time 9 8 2014 1 39 28 PM _ o Test Procedure Result W 1 Check Prerequisites eet MeV Ae ees E 73 53h WY 2 Measure Noise UV Lamp On Time 187h
51. aseline Preparing the HPLC System 1 Turn on the control software and the monitor 2 Turn on the modules Start the control software The screen should show all modules and the system status is Not Ready Turn on the modules that require conditioning a Detector lamp warm up for at least 60 min to get a stable baseline b Column compartment set temperature as required c Pump purge d Sampler prepare the standard isocratic sample into a vial e Solvents fill water and Acetontrile into the solvent bottles Load the default method 6 Pump the water acetonitrile 30 70 mobile phase through the column for 10 min for equilibration Agilent 1290 Infinity II VWD User Manual Preparing the Module 4 Setting up an Analysis 7 Select the menu item Run Control gt Sample Info and enter information about this application Click OK to leave this screen Operator name SYSTEM Data file Path D Agilent ChemStation DATA w Subdirectory G7117 Name Pattern Signal 1 lt Samplelame gt lt Date gt lt Time gt x gt Isacratic Sample 2014 08 27 10 42 06 D Vial Location Eo blank run if no entry Sample name lsacratic Sample Multiplier 1 Figure 13 Sample Info Agilent 1290 Infinity II VWD User Manual 55 4 Preparing the Module Running the Sample and Verifying the Results 1 To start a run select the menu item RunControl gt Run Method 2 This will
52. aximum Ref ntensity 7900 Counts Figure 30 Dark Current Test Results Agilent 1290 Infinity II VWD User Manual 107 8 Test Functions Dark Current Test Dark Spectrum of Unfiltered Sample Intensity Counts Figure 31 Dark Current Test Signals Dark Current Test Failed Dark Current Test Failed Probable cause Suggested actions 1 Defective sample or reference diode Replace optical unit 2 Defective sample or reference ADC board Replace optical unit 3 Defective main board Exchange the main board 108 Agilent 1290 Infinity Il VWD User Manual Test Functions 8 Holmium Oxide Test This test verifies the calibration of the detector against the three wavelength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima The figure below shows a holmium test spectrum The Holmium Oxide Test is available in Agilent Lab Advisor preferred tool The test uses the following holmium maxima e 360 8 nm 418 5 nm 536 4 nm See also Declaration of Conformity for HOX2 Filter on page 224 When to do the Test after recalibration as part of the Operational Qualification Performance Verification procedure or e after flow cell maintenance or repair Agilent 1290 Infinity II VWD User Manual 109 110 Test Functions de Holmium Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm
53. board A ERI E Pumps G7104A Flexible Pump 2 No Yes Yes 1 G7120A High Speed Pump 2 No Yes Yes 1 A Samplers G7129A B ALS 2 Yes Yes No No E G7167A B Multisampler 2 Yes Yes No No E Detectors G71144 B VWD 2 Yes Yes No 1 E G7117A B DAD 2 Yes Yes No 1 E G7115A B DAD 2 Yes Yes No 1 E Others G7116B MCT 2 No No No No No Requires a HOST module via CAN 172 Agilent 1290 Infinity II VWD User Manual Hardware Information 12 The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer USB Universal Series Bus as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are ho
54. cal for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN USB and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN USB and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 166 Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using the following tools latest version should be used Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files on a USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for
55. cell removed If the test is OK exchange the flow cell components Run the holmium oxide filter test If the test fails contact your Agilent service representative Please contact your Agilent service representative 111 8 Test Functions D A Converter DAC Test When Preparations 112 The detector provides analog output of chromatographic signals for use with integrators chart recorders or data systems The analog signal is converted from the digital format by the digital analog converter DAC The DAC test is used to verify correct operation of the digital analog converter by applying a digital test signal to the DAC The DAC outputs an analog signal of approximately 50 mV if the zero offset of the analog output is set to the default value of 5 which can be plotted on an integrator A continuous square wave with an amplitude of 10 uV and a frequency of approximately 1 cycle 24 seconds is applied to the signal The amplitude of the square wave and the peak to peak noise are used to evaluate the DAC test If the analog detector signal is noisy or missing Lamp must be on for at least 10 minutes Connect integrator chart recorder or data system to the detector analog output Agilent 1290 Infinity Il VWD User Manual Test Functions 8 D A Converter DAC Test Running the test with Agilent Lab Advisor 1 Run the D A Converter Test with the Agilent Lab Advisor for further information see Online Help o
56. configuration as an example 18 Agilent 1290 Infinity Il VWD User Manual Introduction to the Variable Wavelength Detector 1 The solvent cabinet is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 4 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other Waste tubes are guided through a channel on the right hand side of the instrument keeping the front access clear from tubes The leak plane provides leak management by catching all internal liquid leaks guiding them to the leak sensor for leak detection and passing them on to the next module below if the leak sensor fails The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached Solvent and condensate is guided through the waste channel into the waste container from the detector s flow cell outlet from the Multisampler needle wash port e from the Sample Cooler condensate from the Seal Wash Sensor from the pump s Purge Valve or Multipurpose Valve The waste tube connected to the leak pan outlet on each of the bottom instruments guides th
57. d are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials Agilent 1290 Infinity II VWD User Manual 57 4 58 Preparing the Module PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a pH range between 1 12 and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfon
58. d fix the cuvette Next Steps 5 Install the cuvette holder in the instrument 6 Perform your Wavelength Verification Calibration see Wavelength Verification Calibration on page 102 to check the correct position of the cuvette holder 132 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Correcting Leaks Correcting Leaks Flow cell area 2 Leak sensor area 1 Figure 39 Correcting leaks When If a leakage has occurred in the flow cell area or at the capillary connections Tools required Description Tissue Wrench 1 4 inch for capillary connections 1 Open the doors 2 Use tissue to dry the leak sensor area 1 3 Observe the capillary connections and the flow cell area 2 for leaks and correct if required Agilent 1290 Infinity lI VWD User Manual 133 9 Maintenance Correcting Leaks 4 Close the doors 134 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Replacing Leak Handling System Parts Replacing Leak Handling System Parts Parts required p n Description 5043 0856 Leak Adapter 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Preparations Open or remove the doors 1 Locate the Leak Adapter 1 and Tubing 2 Next Steps 2 Press the Leak Adapter 1 down and remove it together with the tubing 3 Install the Leak Adapter by pressing it into the Main Cover 4 Insert the Tubing 2 ca 85 mm required for replacem
59. describes the following e introduction and specifications e using and optimizing troubleshooting and diagnose e maintenance e parts identification hardware information e safety and related information Agilent Technologies 2014 Printed in Germany 12 2014 G7114 90000 Rev B fe Agilent Technologies
60. dule is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK 2 Type the following at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt ev C WINDOWS system32 cmd exe Cz gt telnet 134 40 30 205 Figure 60 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switch on page 190 When the connection was established successfully the module responds with the following 2A PROO10OALS Figure 61 A connection to the module is made 3 Type and press enter to see the available commands ev Telnet 134 40 30 205 z Agilent Technologies G4212A PR 0100015 gt command syntax description display help info ay t LAN settings Figure 62 Telnet Commands Agilent 1290 Infinity II VWD User Manual 199 13 LAN Configuration Init Mode TCP IP Prop TCP IP Stat Controllers Figure 63 200 eX Telnet 134 40 30 205 Y Table 22 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X x gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X
61. e switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Table 14 ERI signal distribution Pin Signal Description 1 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler 2 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 3 READY H System is ready for next analysis Receiver is any sequence controller 4 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 5 Not used 6 SHUT DOWN L System has ser
62. e Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F Agilent 1290 Infinity II VWD User Manual 215 14 Appendix Safety Symbols Table 23 Symbols The apparatus is marked with this symbol when the user should refer to A the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage j Indicates dangerous voltages Indicates a protected ground terminal i The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up gt Cooling unit is designed as vapor compression refrigeration system Contains fluorinated greenhouse gas refrigerant according to the Kyoto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warming potential GWP see instrument label Confirms that
63. e Detector Introduction 64 Match the Flow Cell to the Column 65 Set the Detector Parameters 68 Warm up of the Detector 69 Apg Agilent Technologies 63 5 Optimizing the Detector Introduction The detector has a variety of parameters that can be used to optimize performance The information below will guide you on how to get the best detector performance Follow these rules as a start for new applications It gives a rule of thumb for optimizing the detector parameters 64 Agilent 1290 Infinity Il VWD User Manual Optimizing the Detector 5 Match the Flow Cell to the Column The tables below recommend the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution Standard HPLC Applications Column length Typical peak width Recommended flow cell lt 5 cm 0 025 min Micro flow cell 10 cm Semimicro flow cell 7 cm E 1min gt ETTE mn ie ypical flow rate Sa 05 0 2 ml min 2 0 4 ml min EEE 4 0 8 ml min ene 01 5 ml min Internal column diameter 10mm 21mm 3 0 mm 46mm Figure 15 Choosing a Flow Cell Standard HPLC Applications Ultra fast separation with RRLC systems Column ID 2 1mm 3 0 mm 46mm Configuration No damper Damper Damper No mixer Mixer Mixer Flow cell 2 ul 3mm 5 ul 6mm 14 ul 10 mm
64. e sensitive to electrostatic discharge ESD gt To prevent accidental electrostatic discharge when coming into contact with components inside the instrument touch one of the metal housing panels at the front of the instrument Agilent 1290 Infinity II VWD User Manual 123 9 Maintenance Replace the Deuterium Lamp 1 Open the doors 2 Locate the heater fan cover Do not touch the glass bulb with your fingers It may reduce the light output 124 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Replace the Deuterium Lamp 7 Insert the lamp RFID tag on top 1 and fix the screws 8 Reconnect the connector 2 Next Steps 11 Reset the lamp counter as described in the User Interface documentation required for non RFID tag lamps only 12 Turn the lamp ON and give the lamp more than 10 minutes to warm up 13 Perform a Wavelength Re calibration after lamp warm up NOTE After lamp on the detector requires a warm up time of 60 minutes No measurements should be performed during this time Agilent 1290 Infinity II VWD User Manual 125 9 Maintenance Replace the Flow Cell Cuvette Holder Replace the Flow Cell Cuvette Holder When If application needs a different type of flow cell or the flow cell needs repair Tools required Description Wrench 1 4 inch for capillary connections Parts required Description 1 Flow cell For flow cell details see
65. e solvent to a suitable waste container Agilent 1290 Infinity II VWD User Manual 19 Introduction to the Variable Wavelength Detector Waste Concept Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid 20 Agilent 1290 Infinity Il VWD User Manual Introduction to the Variable Wavelength Detector 1 Operating Principle Operating Principle Flow cell Column Figure 6 Hydraulic path Agilent 1290 Infinity II VWD User Manual 21 1 22 Introduction to the Variable Wavelength Detector Operating Principle Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity II VWD User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 24 Physical Specifications 27 Performance Specifications G7114B 28 This chapter gives information on environmental requirements physical and performance specifications ee Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Consideration The detector power supply has wide ranging capabilities see Physical Specifications on page 27 It accepts any line voltage in the above mentioned range Consequently there is no voltage selector
66. ee from contamination cell windows buffers and so on Run the filter motor test to determine if the filter motor assembly is defective If defective please contact your Agilent service representative Run the grating motor test to determine if the grating assembly is defective If defective please contact your Agilent service representative Agilent 1290 Infinity II VWD User Manual 89 7 90 Error Information Grating or Filter Motor Errors Error ID Grating 7800 7801 7802 7803 7804 7805 7806 7808 7809 Filter 7810 7811 7812 7813 7814 7815 7816 The motor test has failed Test 0 Failed Filter motor Test 1 Failed Grating motor During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the error message is generated Probable cause Suggested actions 1 Motor is not connected Please contact your Agilent service representative 2 Defective motor Please contact your Agilent service representative 3 Defective missing grating or filter Please contact your Agilent service representative 4 Cable connector defective Please contact your Agilent service representative Agilent 1290 Infinity Il VWD User Manual Error Information 7 Wavelength test failed Error ID 7890 The automatic wavelength check after lamp ignition has failed When the lamp is switched on the detector waits 1 min to warm up
67. ell 6 mm 5 pL with RFID tag High pressure flow cell 10 mm 14 uL 400 bar with RFID tag Prep flow cell 0 06 mm Prep flow cell 0 3 mm Prep flow cell 3 mm Leak Adapter Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Waste accessory kit Flow Cell to Waste Door left Door right Tubing Clip 141 10 Parts and Materials for Maintenance Standard Flow Cell 10 mm 14 pL Item 142 p n G1314 60186 5062 8522 G1314 65061 G1314 65062 79853 29100 G1314 65066 G1314 65064 79853 68742 G1314 65063 G1314 65065 G1314 44010 0515 4780 Description Standard flow cell 10 mm 14 pL 40 bar with RFID tag Capillary column detector PEEK 600 mm lg 0 17 mm i d 1 16 inch o d Cell Repair Kit includes 2x Gasket 1 2x Gasket 2 2x Window Quartz Cell screw kit Conical spring kit 10 pk Ring 2 kit IN small hole i d 1 mm PEEK 2 pk Gaskets 2 IN small hole i d 1 mm KAPTON 10 pk Window quartz kit 2 pk Gasket 1 kit OUT large hole i d 2 4 mm KAPTON 2 pk Ring 1 kit OUT large hole i d 2 4 mm PEEK 2 pk Clip for RFI ID tag Screw for Clip M2 2 4 5 mm long Agilent 1290 Infinity II VWD User Manual Parts and Materials for Maintenance 10 Standard Flow Cell 10 mm 14 pL if 1 Cell screw 23x 8 2 Conical springs 3 Ring 2 IN 4 Gasket 2 IN 5 Quartz window 6 Gasket 1 OUT 7 Ring 1 OUT 2 3x 8 RFID Clip 00 1 9 Screw Figure 41 Standard Flow Cell A
68. ency of your power distribution matches to the power specification of the individual instrument gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations gt Make all connections to the unit before applying power Note the instrument s external markings described under Safety Symbols on page 216 Ground the Instrument WARNING Missing electrical ground Electrical shock gt If your product is provided with a grounding type power plug the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard gt The ground pin must be firmly connected to an electrical ground safety ground terminal at the power outlet Any interruption of the protective grounding conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury Agilent 1290 Infinity II VWD User Manual 213 14 Appendix Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes Explosion hazard gt Do not operate the instrument in the presence of flammable gases or fumes Do Not Remove the Instrument Cover WARNING Instrument covers removed Electrical shock gt Do Not Remove the Instrument Cover gt Only Agilent authorized personnel are allowed to remove instrument covers Always disconnect the power cables and any e
69. ent between Leak Adapter outlet and Leak Pan 5 Insert close the doors Agilent 1290 Infinity I VWD User Manual 135 9 Maintenance Replace the Module Firmware When Tools required OR Parts required Preparations 136 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN USB FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Module Specific Information Table 11 Module Specific Information G7114B G7114B Initial firmware B 06 70 Compatibility wit
70. erver Parameter Figure 57 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions Agilent 1290 Infinity II VWD User Manual 195 13 LAN Configuration Setup DHCP Software required The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section System Info Controller DE12345678 G4208A Main Revision B 02 12 0001 DAD DE64260019 G1315D Main Revision B 06 41 0002 Resident Revison B 06 40 0007 On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 0030D314F89E TYPE G1315 66565 SER MAC REV AC MFG 2140 0820 48728 no info v information on each module a 10 08 Figure 58 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Inte
71. essively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path Agilent 1290 Infinity II VWD User Manual 121 9 Maintenance Remove and Install the Doors Remove and Install the Doors Parts required p n Description 5067 5737 Door left 5067 5736 Door right The figures shown in this procedure exemplarily show the Infinity II Multisampler module The principle of how to remove and or install doors works in the same way for all Infinity I modules 2 For the Installation of the front door Insert the hinges into their guides and move the door in until the release buttons click into their final position 1 Press the release buttons and pull the front door out y A TaN y 3 2 P t A i 122 Agilent 1290 Infinity II VWD User Manual Maintenance 9 Replace the Deuterium Lamp When Tools required Parts required Preparations If noise or drift exceeds application limits or lamp does not ignite Description Screwdriver Pozidriv 1 PT3 p n Description 1 G1314 60101 Deuterium lamp with RFID tag Turn the lamp OFF Injury by touching hot lamp If the detector has been in use the lamp may be hot gt If so wait for lamp to cool down Injury by sharp metal edges gt Be careful when touching the RFI sheet metal at the rear of the fan There are sharp edges CAUTION Electronic boards and components ar
72. et gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F 48 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For correct installation of your system contact your Agilent service representative Agilent 1290 Infinity Il VWD User Manual Preparing the Module 4 Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid Agilent 1290 Infinity I VWD User Manual 49 4 Preparing the Module Setting up an Analysis This chapter can be used for e preparing the system e to learn the set up of an HPLC analysis and to use it as an instrument check to demonstrate that all modules of the system are correctly installed and connected It is not a test of the instrument performance e Learn about special sett
73. et the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 mL min 2 Flush all tubes with at least 30 mL of solvent Set flow to required value of your application and close the purge valve Pump for approximately 10minutes before starting your application Agilent 1290 Infinity II VWD User Manual 51 4 52 Preparing the Module Requirements and Conditions What You Will Need The table below lists the items you need to have for the set up of the analysis Some of these are optional not required for the basic system Table 7 What you will need Agilent 1200 Infinity Series system Pump plus degassing Autosampler Detector standard flow cell installed Degasser optional Column Compartment optional Agilent CDS System should be correctly set up for LAN communication with the Agilent ChemStation Column Zorbax Eclipse XDB C18 4 6 x 150 mm 5 um 993967 902 or an equivalent column Standard Agilent isocratic checkout sample 01080 68704 Conditions A single injection of the isocratic test standard is made under the conditions given in Table 8 on page 52 Table8 Conditions Flow 1 5 mL min Stoptime 8 min Solvent 100 30 water 70 Acetonitrile Temperature Ambient Wavelength sample 254 nm Injection Vol
74. f acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1290 Infinity Il VWD User Manual 59 4 60 Preparing the Module Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si04 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide Zr0 Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC ap
75. f user interface DIA Converter Test G7114B PPBAU00056 General Test Name D A Converter Test Description The test switches a test signal to the analog output that can be measured using an integrator or strip chart recorder Module G7114B PPBAU00056 1290 VWD Status Done Start Time 9 8 2014 2 13 21 PM Stop Time 9 8 2014 2 13 34 PM Test Procedure Y 1 Check Prerequisites 2 Switch on Analog Output W 3 Switch off Analog Output Figure 34 Converter Test Results ADC1 A ADC CHANNEL 76 40 76 468 76 555 02 04 os os i 12 14 16 18 mi Figure 35 D A Converter DAC Test Example of Integrator Plot Running the Test with Instant Pilot The test can be started via the command line 1 To start the test TEST DAC 1 Reply RA 00000 TEST DAC 1 2 To stop the test TEST DAC 0 Reply RA 00000 TEST DAC 0 Agilent 1290 Infinity II VWD User Manual 113 8 Test Functions D A converter Test Evaluation Test Evaluation The noise on the step should be less than 3 uV Probable cause Suggested actions 1 Bad cable or grounding problem between Check or replace the cable detector and external device 2 Defective detector main board Please contact your Agilent service representative 114 Agilent 1290 Infinity Il VWD User Manual Test Functions 8 Other Lab Advisor Functions Other Lab Advisor Functions EMFs Early Maintenance Feature The EMFs screen allows you to view and manage the
76. face provides two error events related to over current situations on the 5 V and 24 V lines Probable cause Suggested actions 1 The load on the ERI is too high Reduce the load 84 Agilent 1290 Infinity Il VWD User Manual Error Information 7 Detector Error Messages These errors are detector specific UV lamp no current Error ID 7450 The lamp anode current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lower current limit the error message is generated Probable cause Suggested actions 1 Lamp disconnected Ensure the lamp connector is seated firmly 2 Top foam removed while lamp is on Please contact your Agilent service representative 3 Defective or non Agilent lamp Exchange the lamp 4 Defective main board Please contact your Agilent service representative 5 Defective power supply Please contact your Agilent service representative Agilent 1290 Infinity II VWD User Manual 85 7 86 Error Information UV lamp no voltage Error ID 7451 The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operation If the anode voltage falls below the lower limit the error message is generated Probable cause 1 Defective or non Agilent lamp 2 Defective power supply 3 Defective main board Ignition Failed Error ID 7452 Suggested actions Exchange
77. filter check failed 91 grating filter motor defective 90 heater current missing 87 heater failed 93 heater power at limit 94 holmium oxide test failed 89 ignition without cover 83 83 illegal temperature value from sensor at air inlet 93 Agilent 1290 Infinity II VWD User Manual F illegal value from sensor on main board 92 lamp current missing 85 lamp ignition failed 86 lamp voltage missing 86 leak sensor open 81 leak sensor short 81 leak 80 lost CAN partner 80 remote timeout 79 shutdown 78 timeout 78 wavelength check failed 91 fan failed 83 features 10 firmware description 166 initial 137 main system 166 resident system 166 update tool 167 updates 167 136 upgrade downgrade 136 flow cell correction factors 67 high pressure parts 148 insall 126 micro parts 144 remove 126 replace 126 semimicro parts 146 solvent information 223 standard parts 142 with RFID tag 13 flow cells matching for application 65 frequency range 27 G general error messages 78 grating assembly 16 holmium oxide declaration of conformity 224 filter 15 test 109 humidity 27 information on cuvette holder 131 installation bench space 26 install doors 122 flow cell 126 instrument layout 183 interfaces Infinity I 172 internet 225 introduction 10 to optical system 12 L lamp intensity test 97 with RFIDtag 14 LAN configuration ChemStation 205 PC setup 202 LAN Bootp amp Store 192
78. ggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Eye damage by detector light Eye damage may result from directly viewing the UV light produced by the lamp of the optical system used in this product gt Always turn the lamp of the optical system off before removing it 118 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure tha
79. gilent 1290 Infinity II VWD User Manual 143 10 Parts and Materials for Maintenance Micro Flow Cell 3 mm 2 pL Item p n G1314 60187 5021 1823 1 79883 22402 5062 8553 3 79883 28801 4 79883 22301 5 1000 0488 6 G1315 68710 7 79883 68702 8 G1314 44010 9 0515 4780 61314 87301 G1314 87302 G1315 68713 79883 68703 144 Description Micro flow cell 3 mm 2 pL 120 bar with RFID tag Capillary column detector SST 400 mm lg 0 12 mm i d Window screw Washer kit 10 pk Compression washer Window holder Quartz window Gasket FRONT PTFE 1 3 mm hole inlet side 12 pk Gasket BACK PTFE 1 8 mm hole outlet side 12 pk Clip for RFI ID tag Screw for Clip M2 2 4 5 mm long Capillary IN 0 12 mm 310 mm lg Capillary OUT 0 17 mm 120 mm lg Cell repair kit semi micro includes window screw kit Gasket Kit BACK Gasket Kit FRONT and 4 mm hexagonal wrench Window screw kit includes 2 quartz windows 2 compression washers 2 window holders 2 window screws and 10 washers Agilent 1290 Infinity Il VWD User Manual Parts and Materials for Maintenance 10 Micro Flow Cell 3 mm 2 pL 1 1 Window screw 6 2 5x 2 Washers Con 3 Compression washer 4 Window holder 5 Quartz window 6 Gasket front 7 Gasket back 8 RFID clip 9 Screw Figure 42 Micro Flow Cell Agilent 1290 Infinity II VWD User Manual 145 10 Parts and Materials for Maintenance Semi micro Flow Cell 6 mm 5 pL
80. h 1100 1200 series modules When using the G7114B in a system all other modules must have firmware from set 6 50 or above main and resident Otherwise the communication will not work Conversion emulation Possible via Lab Advisor software G1314E F Agilent 1290 Infinity II VWD User Manual 137 9 Maintenance Replace the Module Firmware 138 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity II VWD User Manual 10 Parts and Materials for Maintenance Overview of Maintenance Parts 140 Standard Flow Cell 10mm 14pL 142 Micro Flow Cell3mm 2 pL 144 Semi micro Flow Cell6 mm 5puL 146 High Pressure Flow Cell 10mm 14pL 148 Cuvette Holder 150 Accessory Kit 151 This chapter provides information on parts for maintenance Apg Agilent Technologies 139 10 Parts and Materials for Maintenance Overview of Maintenance Parts Overview of Maintenance Parts Figure 40 Overview of maintenance parts 140 Agilent 1290 Infinity Il VWD User Manual Item p n 1 G1314 60101 2 G1314 60186 2 G1314 60187 2 G1314 60183 2 G1314 60182 2 G1314 60023 2 G1314 60025 2 G1314 60024 3 5043 0856 4 5063 6527 5062 8535 5 5067 5737 6 5067 5736 7 5043 1013 Agilent 1290 Infinity II VWD User Manual Parts and Materials for Maintenance 10 Description Deuterium lamp with RFID tag Standard flow cell 10 mm 14 pL 40 bar with RFID tag Micro flow cell 3 mm 2 uL 120 bar with RFID tag Semi micro flow c
81. ic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Agilent 1290 Infinity Il VWD User Manual Preparing the Module 4 Tantalum Ta Tantalum is inert to most common HPLC solvents and almos
82. ical 6 conical 3 conical 10 conical mm Volume 14 5 2 14 uL Inlet i d 0 25 0 17 0 12 0 25 mm Inlet length 750 250 310 750 mm Outlet i d 0 30 0 17 0 17 0 17 mm Outlet length 120 120 120 120 mm Total volume 60 77 14 49 14 00 60 77 uL Materials in contact SST quartz PTFE SST quartz PTFE SST quartz PTFE SST quartz Kapton with solvent PEEK Agilent 1290 Infinity II VWD User Manual 13 1 Introduction to the Variable Wavelength Detector Lamp The light source for the UV wavelength range is a deuterium lamp As a result of plasma discharge in a low pressure deuterium gas the lamp emits light over the 190 600 nm wavelength range The lamp has an integrated RFID tag that contains the lamp specific information e g part number burn time A RFID tag reader reads out this information and transfers it to the user interface Source Lens Assembly The source lens receives the light from the deuterium lamp and focuses it onto the entrance slit Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a l mm slit For replacement and calibration purposes to optimize the alignment a slit with a hole is needed 14 Agilent 1290 Infinity II VWD User Manual Agilent 1290 Infinity I VWD User Manual Introduction to the Variable Wavelength Detector Filter Assembly The filter assembly is electromechanically actuated During wavelength calibrations it moves into the light path Cut
83. ies on Internet 225 This chapter provides addition information on safety legal and web ae Agilent Technologies 211 14 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards General Do not use this product in any manner not specified by the manufacturer The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions 212 Agilent 1290 Infinity Il VWD User Manual Appendix 14 Before Applying Power WARNING Wrong voltage range frequency or cabling Personal injury or damage to the instrument gt Verify that the voltage range and frequ
84. in the rear of the detector There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Instrument is partially energized when switched off The power supply still uses some power even when the power switch on the front panel is turned OFF Repair work at the detector can lead to personal injuries e g shock hazard when the detector cover is opened and the instrument is connected to power gt To disconnect the detector from the power line unplug the power cord Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 24 Agilent 1290 Infinity Il VWD User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region
85. ings Before Using the System Solvent Information Observe recommendations on the use of solvents in chapter Solvents in the pump s reference manual Priming and Purging the System When the solvents have been exchanged or the pumping system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel between the solvent reservoir vacuum degasser when available in the system and the pump Solvents containing volatile ingredients will slightly lose these Therefore priming of the pumping system is required before starting an application 50 Agilent 1290 Infinity Il VWD User Manual Preparing the Module 4 Table 6 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation lsopropanol Best solvent to flush air out of the system When switching between reverse lsopropanol phase and normal phase both times Best solvent to flush air out of the system After an installation Ethanol or Methanol Alternative to lsopropanol second choice if no lsopropanol is available To clean the system when using Bidistilled water Best solvent to re dissolve buffer buffers crystals Bidistilled water After a solvent change Best solvent to re dissolve buffer crystals After the installation of normal phase Hexane 5 lsopropanol Good wetting properties seals P N 0905 1420 The pump should never be used for priming empty tubings never l
86. ious problem for example leak stops pump Receiver is any module capable to reduce safety risk 7 START L Request to start run timetable Receiver is any module performing run time controlled activities 8 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities Special Interfaces There is no special interface for this module Agilent 1290 Infinity II VWD User Manual 175 12 Hardware Information Interfaces ERI Enhanced Remote Interface ERI replaces the AGP Remote Interface that is used in the HP 1090 1040 1050 1100 HPLC systems and Agilent 1100 1200 1200 Infinity HPLC modules All new 1200 Infinity II products using the FUSION core electronics use ERI This interface is already used in the Agilent Universal Interface Box 2 UIB2 ERI Description The ERI interface contains eight individual programmable input output pins In addition it provides 24 V power and 5 V power and a serial data line to detect and recognize further add ons that could be connected to this interface This way the interface can support various additional devices like sensors triggers in and out and small controllers etc ssszssss SSsa OPmMmOmDmDmMmeOoOmMMmmac DommcpwaDcm Figure 48 Location of the ERI interface example shows a G7114A B VWD 176 Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Pin Enhanced Re
87. ity is converted to an electrical signal by means of the sample photodiode Part of the light is directed to the reference photodiode by the beam splitter to obtain a reference signal for compensation of intensity fluctuation of the light source A slit in front of the reference photodiode cuts out light of the sample bandwidth Wavelength selection is made by rotating the grating which is driven directly by a stepper motor This configuration allows fast change of the wavelength The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light Lens Sample diode oO Flow cell Beam splitter Reference diode a Filter assembly Entrance slit Mirror M1 Grating Mirror M2 12 Figure 2 Optical Path of the Variable Wavelength Detector Agilent 1290 Infinity II VWD User Manual Introduction to the Variable Wavelength Detector 1 Flow Cell A variety of flow cell cartridges can be inserted using the same quick and simple mounting system The flow cells have an integrated RFID tag that contains the flow cell specific information e g part number cell volume path length A RFID tag reader reads out this information and transfers it to the user interface E RFID tag j yA Figure 3 Flow Cell with RFID tag Table 1 Flow Cell Data STD Semi micro Micro High Pressure Maximum pressure 40 4 40 4 120 12 400 40 bar MPa Path length 10 con
88. ld cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions Agilent 1290 Infinity II VWD User Manual 217 14 Appendix Refrigerant The refrigerant HFKW 134a is used only in the Agilent Infinity II Sample Cooler Table 24 Physical properties of refrigerant HFKW 134a Molecular weight 102 Critical temperature 101 1 C Critical pressure 40 6 bar Boiling point 26 5 C Refrigerant te Refrigerant HFKW 134a is known as a safe refrigerant however accidents can occur if it is handled incorrectly For this reason the following instructions must be observed gt Avoid contact with liquid refrigerant HFKW 134a At atmospheric pressure HFKW 134a evaporates at approximately 26 C and causes frost bite After skin contact rinse the affected area with water gt After eye contact rinse the eye s with plenty of water for at least 15 minutes and consult a doctor gt HFKW 134a must not be allowed to escape in enclosed areas Although HFKW 134a is not toxic there is a danger of suffocation as gaseous refrigerant is heavier than air gt Please observe the following first aid instructions After inhalation move the affected person to fresh air keep him warm and allow him to rest If necessary he should be supplied with oxygen If he has stopped breathing or is breathing erratically he should be given artificial respiratio
89. le Agilent 1290 Infinity II VWD User Manual 191 13 LAN Configuration 192 Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 54 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp Agilent 1290 Infinity Il VWD User Manual LAN Configura
90. le Ring 2 PEEK RFID tag oN ope Figure 37 Standard Flow Cell 1 Disassembling the Flow Cell a Unscrew the cell screw using a 4 mm hexagonal wrench b Remove the SST rings using a pair of tweezers CAUTION Scratched window surfaces by tweezers Window surfaces can easily be scratched by using tweezers for removing the windows gt Do not use tweezers to remove windows c Use adhesive tape to remove the peek ring the window and the gasket d Repeat step a through step c for the other window keep the parts separate otherwise they could be mixed Agilent 1290 Infinity II VWD User Manual 129 9 Maintenance 130 2 Cleaning the Flow Cell Parts a Pour isopropanol into the cell hole and wipe clean with a piece of lint free cloth b Clean the windows with ethanol or methanol Dry it with a piece of lint free cloth Always use new gaskets 3 Reassembling the Flow Cell a Hold the flow cell cassette horizontally and place gasket in position Ensure both cell holes can be seen through the holes of gasket The semi micro 1 and 2 gaskets items 6 and 7 Semi micro Flow Cell 6 mm 5 uL on page 146 look very similar Do not mix them up b Place the window on gasket c Place the peek ring on the window d Insert the conical springs Make sure the conical springs point towards the window Otherwise tightening the cell screw might break the window Conical springs ro my MTN Ring Window
91. limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Lamp Type Counter Reset Comment lamp with RFID tag NO lamp without RFID tag YES via Lab Advisor or Instant Pilot The detector provides the following EMF counters e Deuterium Lamp On Time e Number of UV lamp ignitions Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Agilent 1290 Infinity II VWD User Manual Hardware Information 12 Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled This function is only available via Agilent Lab Advisor or Instant Pilot Agilent
92. ll Exchange flow cell components Agilent 1290 Infinity Il VWD User Manual Error Information 7 Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the Power Please contact your Agilent service Switch board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Agilent 1290 Infinity II VWD User Manual 81 7 82 Error Informati
93. mation 57 Agilent 1290 Infinity II VWD User Manual Contents 5 Optimizing the Detector 63 Introduction 64 Match the Flow Cell to the Column 65 Set the Detector Parameters 68 Warm up of the Detector 69 6 Troubleshooting and Diagnostics 71 Available Tests versus Interfaces 72 Agilent Lab Advisor Software 73 7 Error Information 75 What Are Error Messages 77 General Error Messages 78 Detector Error Messages 85 8 Test Functions 95 Introduction 96 Intensity Test 97 Cell Test 100 Wavelength Verification Calibration 102 ASTM Drift and Noise Test 104 Quick Noise Test 105 Dark Current Test 107 Holmium Oxide Test 109 D A Converter DAC Test 112 Other Lab Advisor Functions 115 9 Maintenance 117 Introduction to Maintenance 118 Warnings and Cautions 118 Overview of Maintenance 120 Cleaning the Module 121 Remove and Install the Doors 122 Replace the Deuterium Lamp 123 Replace the Flow Cell Cuvette Holder 126 Repairing the Flow Cells 128 Agilent 1290 Infinity II VWD User Manual Contents Using the Cuvette Holder 131 Correcting Leaks 133 Replacing Leak Handling System Parts 135 Replace the Module Firmware 136 10 Parts and Materials for Maintenance 139 Overview of Maintenance Parts 140 Standard Flow Cell10mm 14uL 142 Micro Flow Cell3mm 2puL 144 Semi micro Flow Cell6mm 5uL 146 High Pressure Flow Cell 10mm 14pL 148 Cuvette Holder 150 Accessory Kit 151 1 Identifying Cables 153 Cable O
94. mote 1 10 1 START D Sub female 15way EGET user s view to connector is ail Jet eae es eek 2 10 2 STOP oOo O 0 QO Oo Q Q OQO oo N toa ul A w N e s 3 10 3 READY eeeee0 4 10 4 POWER ON 15 O T 5 10 5 NOT USED 22565655F 6 10 6 SHUT DOWN e 3 7 10 7 START 8 10 8 PREPARE 9 1 wire DATA 10 DGND 11 5 V ERI out 12 PGND 13 PGND 14 24 V ERI out 15 24 V ERI out 10 Input Output Lines e Eight generic bi directional channels input or output e Same as the APG Remote e Devices like valves relays ADCs DACs controllers can be supported controlled 1 Wire Data Future Use This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI device The firmware can detect the connected type of device automatically and update information in the device if required Agilent 1290 Infinity II VWD User Manual 177 12 Hardware Information 5V Distribution Future Use Available directly after turn on oft the hosting module assures that certain base functionality of the device can be detected by firmware For digital circuits or similar Provided 500 mA maximum Short circuit proof with automatic switch off by firmware 24V Distribution Future Use Available by firmware command defined turn on off For devices that need higher power Class 0 0 5 A maximum 12 W Class 1 1 0 A maximum 24 W Class 2 2 0 A maximum 48 W Class depends on hosting module
95. n In the case of cardiac arrest carry out heart massage Send for a doctor immediately gt Moreover it must be noted that HFKW 134a must always be extracted from the system and collected It must never be discharged into the atmosphere on environmental grounds greenhouse effect 218 Agilent 1290 Infinity Il VWD User Manual Appendix 14 CAUTION General hazards and improper disposal Improper disposal of the media and components used pollutes the environment gt The breakdown of the sample cooler unit must be carried out by specialist refrigeration company gt All media must be disposed of in accordance with national and local regulations gt Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance Agilent 1290 Infinity II VWD User Manual 219 14 Appendix Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as
96. nate effects due to absorbing solvents the test should be done with water in the flow cell Limits No real limit The reason is that it depends on the position alignment of the reference side beam splitter reference slit reference diode Therefore the reference side value can be higher smaller than the sample side value With a clean cell the counts for sample and reference photocurrent are in the same range If the sample side shows much lower values than the reference side the flow cell might have a problem Flush the flow cell with a flow of 1 mL min for at least 10 minutes Agilent 1290 Infinity Il VWD User Manual Test Functions 8 Cell Test 1 Run the Cell Test with Agilent Lab Advisor for further information see Online Help of user interface Cell Test G7114B PPBAU00056 General Test Name Cell Test Description Calculate the ratio of the sample signal and the reference signal measured in the zero order of the grating Module G7114B PPBAU00056 1290 VWD Status Passed Start Time 9 8 2014 1 26 58 PM Stop Time 9 8 2014 1 27 05 PM OO Test Procedure ae Name Value WY 1 Check Prerequisites Accumulated UV Lamp Bum Time RANI 2 Fush Fow Cel UV Lamp On Time 1 74h 3 Measure Sample and Reference Intensity Intensity Sample 1 932 161 Counts Yy 4 Evaluate Data Intensity Reference 1 965 528 Counts Intensity Ratio 0 98 Figure 24 Test Results Cell Test Failed Cell Test Failed
97. nstrument Method More VWD System On System Off Revisions amp Serial s Columns Instrument Configuration i Sequences I lgh defies solvent is stored in the memory EE E o Step 2 Sample Scan scan of the peak of Sa interest is taken while the peak stays in the flow alance ea PRs cell stop flow condition UV Lamp Switch Off e Online Spectrum Sample Scan minus Blank S Scan Here the functions are inactive grayed out Will be active in run mode 4 When the peak of interest enters the flow cell stop the flow set flow rate to zero or open the purge valve and wait a few moments to stabilize the concentration Turning off the pump would stop the run and no access to the sample scan is possible Agilent 1290 Infinity II VWD User Manual 45 3 Using the Module 5 Open the Online Spectra window View gt Online Spectra gt VWD and change the absorbance and wavelength range according your needs EE VWD Scan mAU o amp fs mau 800 e00 400 0 rp 100 200 200 400 50 a 100 00 to 1000 02 mau Bal f1 to f1000 nm Figure 11 Online Spectra Window TISAI AA A LORE ALL LA DL A A 600 700 800 900 nm 6 Select Sample Scan A sample scan is taken in the range defined under Miscellaneous in Table 4 on page 42 and displays the result Sample Scan minus Blank Scan 46 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infini
98. nt Lab Advisor 73 Agilent ChemStation Setup 202 oninternet 225 algae 223 ambient non operating temperature 27 ambient operating temperature 27 analog signal 174 ASTM Drift 104 beam splitter 16 Beer Lambert absorbance 66 bench space 26 boards photodiode boards ADC 16 Bootp amp Store 192 using default 193 using stored 193 C cable CAN 162 LAN 162 overview 154 RS 232 163 cables analog 156 226 remote 158 CAN cable 162 cautions and warnings 118 cell test 100 ChemStation setup 205 chromatogram 53 cleaning 121 compatibility 137 compensation sensor open 82 compensation sensor short 82 condensation 26 configuration switche 190 conversion 137 correction factors for flow cells 67 cutoff filter 15 cuvette holder 131 parts 150 D D A converter test 112 D A converter 112 DAC 112 Instant Pilot 113 dark current test 107 declaration of conformity 224 detector error messages 85 detector set up Open Lab ChemStation 37 detector warmup 69 detector preparation 54 DHCP general information 195 setup 196 diagnostic test functions 95 dimensions 27 doors install 122 remove 122 E electrical connections descriptions of 169 electronic waste 220 EMF early maintenance feedback 184 emulation 137 entrance slit assembly 14 environment 26 error message ADC hardware error 92 error messages calibration failed 88 compensation sensor open 82 compensation sensor short 82 detector 85 fan failed 83
99. ntative Agilent 1290 Infinity II VWD User Manual 93 7 94 Error Information Heater Power At Limit Error ID 1074 The available power of the heater reached either the upper or lower limit This event is sent only once per run The parameter determines which limit has been hit 0 means upper power limit hit excessive ambient temperature drop 1 means lower power limit hit excessive ambient temperature increase Probable cause Suggested actions 1 Excessive ambient temperature change Wait until temperature control equilibrates Cover Violation Error ID 7461 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity I VWD User Manual 8 Test Functions Introduction 96 Intensity Test 97 Intensity Test Evaluation 98 Intensity Test Failed 99 Cell Test 100 Cell Test Failed 101 Wavelength Verification Calibration 102 When to Calibrate the Detector 102 ASTM Drift and Noise Test 104 Quick Noise Test 105
100. ntensity Test UV Lamp On Time 1 64h 3 Evaluate Data Lowest Intensity 28565 Counts Lowest Intensity Limit 6400 Counts Average Intensity 981399 Count Av nsity Limit 160000 Counts Highest Intensity 6929621 Counts Highest Intensity Limit 320000 Counts Figure 22 Intensity Test Results Intensity Spectrum Intensity Counts 6000000 4000000 2000000 0 a ee ee 200 300 400 500 600 700 800 Wavelength nm Figure 23 Intensity Test Signal 98 Agilent 1290 Infinity Il VWD User Manual Intensity Test Failed Intensity Test Failed Probable cause 1 Empty flow cell 2 Flow cell windows dirty 3 Optics defect 4 Defective lamp or optics Agilent 1290 Infinity II VWD User Manual Test Functions Suggested actions Ensure the flow cell is filled with water Repeat the test with the flow cell removed If the test passes exchange the flow cell windows Please contact your Agilent service representative Exchange the lamp 99 8 Test Functions Cell Test Prerequisites 100 The cell test compares the intensity of the deuterium lamp measured by the sample and reference diodes unfiltered and not logarithmized when the grating is in the zero order position The resulting intensity ratio sample reference is a measure of the amount of light absorbed by the flow cell The test can be used to check for dirty or contaminated flow cell windows When the test is started the gain is set to 1 To elimi
101. of the expected value This indicates the detector is calibrated correctly 1 Run the Holmium Oxide Test with the Agilent Lab Advisor for further information see Online Help of user interface AW 1 Check Prerequisites WY 2 Perform Holmium Oxide Test 3 Evaluate Data Holmium Oxide Test G7114B PPBAU00056 General Limits_ Signals Test Name Holmium Oxide Test Module G7114B PPBAU00056 1290 VWD Status Passed Start Time 9 8 2014 2 59 53 PM Stop Time 9 8 2014 3 01 07 PM al Test Procedure Description The test measures the Holmium spectrum from the builtin Holmium filter The spectrum is evaluated for peaks at different wavelengths Result Name Accumulated UV Lamp Bum Time UV Lamp On Time 3 29h Holmium Deviation to 360 8nm 0 40 nm Holmium Deviation Limit Inm Holmium Deviation to 418 5nm 0 70nm Holmium Deviation to 536 4nm 0 00 nm Figure 32 Holmium Oxide Test Results Absorbance AU Spectrum of Holmium Filter Test Wavelength nm Figure 33 Holmium Oxide Test Signals Agilent 1290 Infinity Il VWD User Manual Holmium Oxide Test Failed Holmium Oxide Test Failed Probable cause 1 Detector not calibrated 2 Dirty or defective flow cell 3 Dirty or defective holmium oxide filter 4 Optical misalignment Agilent 1290 Infinity II VWD User Manual Test Functions Suggested actions Recalibrate the detector Repeat the test with the flow
102. off Filter plus Holmium Oxide Filter Shutter Cutoff Filter Figure 4 Filter Assemby The filter assembly has two filters installed and is processor controlled OPEN nothing in light path at A lt 370 nm CUTOFF cut off filter in light path at A gt 370 nm HOLMIU holmium oxide filter for wavelength check M SHUTTER for measurement of dark current of photo diodes A photo sensor determines the correct position 15 1 Introduction to the Variable Wavelength Detector Mirror Assemblies M1 and M2 The instrument contains two spherical mirrors M1 and M2 The beam adjustable is vertically and horizontally Both mirrors are identical Grating Assembly The grating separates the light beam into all its component wavelengths and reflects the light onto mirror 2 The stepper motor reference position is determined by a plate fitted onto the motor shaft interrupting the beam of a photo sensor The wavelength calibration of the grating is done at the zero order light position and at 656 nm which is the emission line of the deuterium lamp Beam Splitter Assembly The beam splitter splits the light beam One part goes directly to the sample diode The other part of the light beam goes to the reference diode Photo Diodes Assemblies Two photo diode assemblies are installed in the optical unit The sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in
103. on Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Loose connection between the power Please contact your Agilent service switch board and the main board representative 2 Defective power switch board Please contact your Agilent service representative Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective power switch board Please contact your Agilent service representative 2 Loose connection between the power Please contact your Agilent service switch board and the main board representative Agilent 1290 Infinity Il VW
104. onnection has limited or no connectivity Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration i Internet Protocol TCP IP Properties Agilent 1290 Infinity I VWD User Manual 203 13 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross over LAN cable with the module s IP address 4 Click on OK to save the configuration 204 Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 Agilent ChemStation Setup This procedure describes the Agilent ChemStation B 04 02 setup for the 1290 Infinity system using the 1290 Infinity DAD G4212A as the interfacing module The setup works in the same way for all other systems The LAN must be connected to detector due to high data load on communication to Control Software 1 Open the ChemStation Configuration Editor iojxi File Configure Utilities Help Orstnmert 1 4 LC 3D Data Analysis only 2 Select from the menu Configure Instruments 3 Select Modular 3D LC System 4 Give the Instrument a name Agilent 1290 Infinity II VWD User Manual 205 13 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK Select Instrument Initially Start Instrument Session Yes No Dual Channel Iface 359008 CTCAutoSampler CTC PAL
105. own 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1290 Infinity II VWD User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo eee S S te Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low Agilent 1290 Infinity II VWD User Manual 161 11 Identifying Cables CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 162 Agilent 1290 Infinity Il VWD User Manual RS 232 Cables p n G1530 60600 RS232 61601 5181 1561 Identifying Cables 11 Description RS 232 cable 2 m RS 232 cable 2 5 m
106. perating Principle 21 This chapter gives an introduction to the detector instrument overview and internal connectors ott Agilent Technologies 9 1 Introduction to the Variable Wavelength Detector Introduction to the Detector The Agilent variable wavelength detectors described in this manual are designed for highest optical performance GLP compliance and easy maintenance with 10 higher data rate up to 240 Hz for ultra fast HPLC G7114B deuterium lamp for highest intensity and lowest detection limit over a wavelength range of 190 to 600 nm optional flow cell cartridges standard 10 mm 14 uL high pressure 10 mm 14 uL micro 3 mm 2 uL semi micro 6 mm 5 uL and Prep Cells are available and can be used depending on the application needs other types may be introduced later Dual wavelength mode see Dual Wavelength Mode on page 17 easy front access to lamp and flow cell for fast replacement electronic identification of flow cell and lamp with RFID Radio Frequency Identification tag for unambiguous identification lamp information part number serial number production date ignitions burn time cell information part number serial number production date nominal path length volume maximum pressure built in electronic temperature control ETC for improved baseline stability and built in holmium oxide filter for fast wavelength accuracy verification Agilent 1290 Infinity Il VWD User Manual
107. pha line Calibration 0 000 nm Do you want to calibrate the detector using the wavelength verification results R Figure 25 Wavelength Calibration Results If you select No the test is aborted If you select Yes the re calibration is performed the offset is corrected Agilent 1290 Infinity II VWD User Manual 103 8 Test Functions ASTM Drift and Noise Test The ASTM Drift and Noise test determines the detector noise over a period of 20 minutes The test is done with HPLC grade water flowing through the flow cell at 1 mL min On completion of the test the noise result is displayed automatically 1 Run the ASTM Drift and Noise Test with Agilent Lab Advisor for further information see Online Help of user interface ASTM Drift and Noise Test G7114B PPBAU00056 General Limits_ Signals Test Name ASTM Drift and Noise Test Description The test performs ASTM drift and noise evaluation Module G7114B PPBAU00056 1290 VWD Status Passed Start Time 9 8 2014 12 42 48 PM Stop Time 9 8 2014 1 02 49 PM Test Procedure Result Name 1 Check Prerequisites 72 67h Accumulated UV Lamp Bum Time 2 Measure Noise UV Lamp On Time 1 01h W 3 Evaluate Data Minimum Lamp On Time 1H Signal Drift value at 254 nm UV 0 477 mAU h Maximum Allowed Drift 1 1 MAU h Signal Noise value at 254 nm UV 0 004 mAU mum Allowed Noise 0 0 02 mAU Figure 26 ASTM Drift
108. plications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except 1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Agilent 1290 Infinity Il VWD User Manual Preparing the Module 4 Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al Og are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Agilent 1290 Infinity II VWD User Manual 61 4 Preparing the Module Solvent Information 62 Agilent 1290 Infinity II VWD User Manual This chapter provides information on how to optimize the detector Agilent 1290 Infinity II VWD User Manual 5 Optimizing th
109. re furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or
110. reen Pre run Post run purple Run blue Idle green Offline dark gray Standby light gray i VWD OKO 250 nm a Idle B G Control EA Method Error Method Identify Device pa Balance a Switch Off Agilent 1290 Infinity II VWD User Manual A right click into the Active Area will open a menu to Show the Control Interface special module settings Show the Method interface similar as via menu Instrument gt Setup Instrument Method Set Error Method Identify Module Status LED will blink e Perform a Balance Switch the UV lamp on off same as click on button Make Device Ready Turn device off standby Take Abort Scans during flow off 39 3 Using the Module Detector Control Settings Detector Control Settings 40 automatic mode only if lamp tag is available manual mode lamp type is VWD lamp manual mode lamp type is DAD lamp use Cell only if RFID Tag is available use cell anyway The figure shows the default settings Lamps can be turned ON OFF Analog Output Range can be set to either 100 mV or 1 Vfull scale for additional settings see Analog under Table 4 on page 42 Temperature Control The optical unit is kept on constant temperature some degrees above ambient and improves the baseline stability in unstable environments see also Environment on page 26 If the flow cell temperature is
111. ress Enter to exit with storing parameters WINDOWS system32 cmd exe Technologies G4212A PROG168615 exit Connection to host lost AEO n Figure 65 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 192 168 254 12 Agilent 1290 Infinity II VWD User Manual 201 13 LAN Configuration and Agilent ChemStation Setup PC and Agilent ChemStation Setup 202 PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see Table 19 on page 194 1 Open the Local Area Connection Properties and select Internet Protocol TCP IP Then click on Properties 4 Local Area Connection Properties 2 xi General Advanced Connect using B Broadcom NetXtreme Gigabit Etheme Configure This connection uses the following items Client for Microsoft Networks v 5 File and Printer Sharing for Microsoft Networks M E QoS Packet Scheduler Internet Protocol TCP IP Install Uninstall Description Transmission Control Protocol Intermet Protocol The default wide area network protocol that provides communication across diverse interconnected networks IV Show icon in notification area when connected IV Notify me when this c
112. rface Card or the main board of above mentioned modules 196 Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 Table 20 G1369C LAN Interface Card configuration switch on the card SW 4 SW5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP Table 21 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation Lab Advisor Firmware Update Tool and use MAC address as host name e g 003043177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 195 Agilent 1290 Infinity II VWD User Manual 197 13 LAN Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 59 Manual Configuration Principle 198 Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 With Telnet Whenever a TCP IP connection to the mo
113. rformance Specifications Feature Specification Flow cells Standard 14 pL volume 10 mm cell path length and 40 bar 588 psi pressure maximum Micro 2 uL volume 3 mm cell path length and 120 bar 1760 psi pressure maximum Semi micro 5 pL volume 6 mm cell path length and 40 bar 588 psi pressure maximum Preparative 4 uL volume 3 mm cell path length and 120 bar 1760 psi pressure maximum Preparative 0 3 mm cell path length and 50 bar 725 psi pressure maximum Preparative 0 06 mm cell path length and 50 bar 725 psi pressure maximum Spectral tools Stop flow wavelength scan Analog output Recorder Integrator 100 mV or 1 V 1 output Communication LAN Controller area network CAN ERI ready start stop and shut down signals GLP Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic records of maintenance and errors RFID for electronics records of flow cell and UV lamp conditions path length volume product number serial number test passed and usage Verification of wavelength accuracy with built in holmium oxide filter Safety and Extensive diagnostics error detection and display through Agilent Instant maintenance Pilot and Agilent Lab Advisor software Leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Tracking of flow cells
114. s calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate e after maintenance flow cell or lamp e after repair of components in the optical unit e after exchange of the optical unit or VWM board e at a regular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and e when chromatographic results indicate the detector may require recalibration Agilent 1290 Infinity Il VWD User Manual Test Functions 8 Wavelength Verification Calibration NOTE If the detector was repaired opened covers the wavelength calibration can be done 10 minutes after lamp on A final wavelength calibration should be repeated after complete warm up of the detector 1 Run the Wavelength Calibration with the Agilent Lab Advisor for further information see Online Help of user interface Wavelength Calibration G7114B PPBAU00056 Wavelength Calibration Description This procedure performs a Wavelength Verification and G7114B PPBAU00056 Test Procedure g Value WY 1 Check Prerequisites z ETT 2 Wavelength Verification UV Lamp On Time 228h u 3 Calibrate Detector Time to Wait Before Wavelength Calibration Can Be Stated 0 00 min Time to Wait Before Wavelength Calibration Can Be Started 0 0 min Wavelength Deviation of O order Calibration 0 200 nm Wavelength Deviation of al
115. s for Agilent VWD flow cells Part number Path length actual Correction factor Standard flow cell 10 mm 14 uL 40 bar G1314 60186 10 15 0 19 mm 10 10 15 Semi micro flow cell 6 mm 5 pL G1314 60183 6 10 0 19 mm 6 6 10 Micro flow cell 3 mm 2 uL 120 bar G1314 60187 2 80 0 19 mm 3 2 8 High pressure flow cell 10 mm 14 pL 400 bar G1314 60182 10 00 0 19 mm 10 10 However you have to be aware that there is additional tolerance of gasket thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance Agilent 1290 Infinity II VWD User Manual 67 5 Optimizing the Detector Set the Detector Parameters 1 Set peakwidth as close as possible to the width at half height of a narrow peak of interest 2 Choose the sample wavelength at a longer wavelength than the cut off wavelength of the mobile phase at a wavelength where the analytes have strong absorptivity if you want to get the lowest possible detection limit at a wavelength with moderate absorptivity if you work with high concentrations and preferably where the spectrum is flat for better linearity 3 Consider to use time programming to further optimization 68 Agilent 1290 Infinity Il VWD User Manual Optimizing the Detector 5 Warm up of the Detector Give the optical unit enough time to warm up and stabilize gt 60 minutes The detector is temperature controlled After turn on of the detector
116. s of National Institute of Standards and Technology NIST to be applied as certified wavelength standard According to the publication of NIST in J Res Natl Inst Stand Technol 112 303 306 2007 the holmium oxide glass filters are inherently stable with respect to the wavelength scale and need no recertification The expanded uncertainty of the certified wavelength values is 0 2 nm Agilent Technologies guarantees as required by NIST that the material of the filters is holmium oxide glass representing the inherently existent holmium oxide absorption bands Test wavelengths Where x can be any alphanumeric character Wavelength Accuracy Bandwidth G1315x G1365x 1100 1200 1260 1 nm 2 nm G7115x G7165x 1260 G1600x G7100x CE G1314x 1100 1200 1260 1290 360 8um 418 5nm G7114x 1260 1290 36 4nm G4286x 94x 1120 1220 The variation in Measured Wavelength depends on the different Optical Bandwidth 28 Oct 2014 Sn Bogarta R amp D Manager Quality Manager P N 89550 90501 Revision G Effective by 28 Oct 2014 Agilent 1290 Infinity II VWD User Manual Appendix 14 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent 1290 Infinity II VWD User Manual 225 Index Index A absorbance Lambert Beer 66 Agilent Lab Advisor software 73 Agile
117. s with their parameters over the run time Add lines as required Time Limits 0 00 to 99999 00 min in steps of 0 01 min Via the buttons in the bottom area time table lines can be added removed cut copied pasted or completely cleared Based on the chosen function a certain parameter can be selected Instrument Curves The detector has several signals internal temperatures voltages of lamps that can be used for diagnosing problems These can be baseline problems deriving from EEUE ASS deuterium lamps wander drift problems due to Uy Dawe Anado voltene y temperature changes PEI SI eee These signals can be used in addition to the normal baseline TESTS GES signal to determine whether correlation to temperature or voltage current of the lamp These signals are available via the Agilent ChemStation Online Plot Data Signal and or Agilent Lab Advisor Software 44 Agilent 1290 Infinity Il VWD User Manual Using the Module 3 Scanning with the VWD Table 5 Blank scan Access to the scan feature is only possible during run with stopped flow The spectrum is taken during a stop flow condition only while the peak is kept in the flow cell 1 Set up a run 2 Start a run 3 While running on the baseline take a Blank Scan A background scan is stored in the memory Method Sequence View Abort Help Step 1 Blank Scan scan of the background Select Injection Source Set up I
118. start the modules and the online plot on the Agilent ChemStation will show the resulting chromatogram DAD1 A Sig 254 4 Ref 500 100 04101410140003 D mau pot es ae 5 LST a a a aa ae ep et ae ap min Figure 14 Chromatogram with lsocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your ChemStation manual supplied with your system 56 Agilent 1290 Infinity Il VWD User Manual Preparing the Module 4 Solvent Information Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see pump manuals e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources an
119. sted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler Agilent 1290 Infinity II VWD User Manual 173 12 Hardware Information 174 USB The USB interface replaces the RS 232 Serial interface in new FUSION generation modules For details on USB refer to USB Universal Serial Bus on page 178 Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Remote ERI The ERI Enhanced Remote Interface connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules ar
120. switch see Table 18 on page 191 Agilent 1290 Infinity II VWD User Manual 189 13 LAN Configuration Configuration Switch Configuration Switch The configuration switch can be accessed at the rear of the module Configuration switch Figure 52 Location of Configuration switch example shows a G7114A B VWD The module is shipped with all switches set to OFF as shown above NOTE To perform any LAN configuration SW1 and SW2 must be set to OFF Table 17 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Initialization Mode Selection on page 191 190 Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 Initialization Mode Selection The following initialization init modes are selectable Table 18 Initialization Mode Switches SW1 SW2 SW3 SW4 SW5 SW 6 Init Mode 0 0 0 0 0 0 Use Default IP Address 0 0 0 0 1 0 Use Stored IP Address 0 0 0 1 0 0 Use DHCP Default IP address for LAN is 192 168 254 11 DHCP address is the module s LAN MAC address Bootp When the initialization mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp gt Active Server Parameter Figure 53 Bootp Princip
121. t all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens e High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCl Oo 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution o
122. t you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment Agilent 1290 Infinity II VWD User Manual 119 9 Maintenance Overview of Maintenance Table 10 Simple Repairs The following pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Procedures Typical Frequency Notes Deuterium lamp exchange Flow cell exchange Cleaning flow cell parts cleaning or exchange Leak sensor drying Leak handling system replacement If noise and or drift exceeds your application limits or lamp does not ignite If application requires a different flow cell type If leaking or if intensity drops due to contaminated flow cell windows If leak has occurred If broken or corroded A wavelength calibration test and an intensity test should be performed after replacement A wavelength calibration test should be performed after replacement A pressure tightness test should be done after repair Check for leaks Check for leaks 120 Agilent 1290 Infinity Il VWD User Manual Maintenance 9 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an exc
123. tch 4 UP and others DOWN module uses DHCP 2 Enter the setup information MAC IP address and or Instrument Name a Agilent OpenLab ChemStation Configure Instrument Agilent LC Modules and Systems dh Fraction Colector Sa Frection Collector Guster ha Low Row Fraction Colecter Qm G e soie Q io rov soroa OK Cancel QD Lew ew HP Sampler QD reo secier we Puro A Hoiame 0030X6595 Agilent 1290 Infinity II VWD User Manual 35 3 Using the Module Instrument Configuration b Lab Advisor Instrument Overview Add Instrument System System Name G7117B 36 Agilent 1290 Infinity II VWD User Manual Using the Module 3 Set up the Detector with Agilent Open Lab ChemStation The setup of the detector is shown with the Agilent OpenLab ChemStation C 01 06 This section describes the detector settings only For information on the Agilent OpenLab ChemStation or other 1200 Infinity modules refer to the corresponding documentation WAU ord SSS Tea RenConrl ener Mathed _Seqemne Viar Abe lp Instrument Idle Basti leche Twain Ji d Figure 7 ChemStation Method and Run Control just detector is shown After successful load of the OpenLab ChemStation you should see the module as an active item in the graphical user interface GUD Agilent 1290 Infinity II VWD User Manual 37 3 Using the Module The Detector User Interface The Detector User Interface Within the
124. ter at fan assembly failed 93 he Agilent Technologies 75 7 Error Information Heater Power At Limit 94 Cover Violation 94 This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions 76 Agilent 1290 Infinity Il VWD User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface
125. ter describes the detector s built in test functions 9 Maintenance This chapter provides general information on maintenance of the detector 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the detector in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC 14 Appendix This chapter provides addition information on safety legal and web Agilent 1290 Infinity II VWD User Manual Contents Contents Introduction to the Variable Wavelength Detector 9 Introduction to the Detector 10 Product Description 11 Optical System Overview 12 Dual Wavelength Mode 17 Leak and Waste Handling 18 Operating Principle 21 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications G7114B 28 Using the Module 31 Magnets 32 Turn on off 33 Status Indicators 34 Instrument Configuration 35 Set up the Detector with Agilent Open Lab ChemStation 37 The Detector User Interface 38 Detector Control Settings 40 Method Parameter Settings 41 Scanning withthe VWD 45 Preparing the Module 47 Leak and Waste Handling 48 Setting up an Analysis 50 Solvent Infor
126. the front of the optical unit Photo Diode ADC analog to digital converter The photo diode current is directly converted to digital data direct photo current digitalization The data is transferred to the detector main board The photo diode ADC boards are located close to the photo diodes 16 Agilent 1290 Infinity Il VWD User Manual Introduction to the Variable Wavelength Detector 1 Dual Wavelength Mode The detector provides a Dual Wavelength mode that offers additional operation functions Features e 200 ms acquisition time per data point 5 Hz data rate distributed to two channels 2 5 Hz data rate for each channel e delta wavelength max 150 nm scans during Dual Wavelength mode are possible e the second order filter is disabled when one wavelength is lt 370 nm Timetable Wavelength settings are timetable programmable depends if enough time for implementation is available e switching from Single Wavelength mode to Dual Wavelength mode is NOT timetable programmable e filter settings are not timetable programmable Agilent 1290 Infinity II VWD User Manual 17 1 Introduction to the Variable Wavelength Detector Leak and Waste Handling Leak and Waste Handling The 1290 Infinity II Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed Figure 5 Leak and waste handling concept overview typical stack
127. the lamp Please contact your Agilent service representative Please contact your Agilent service representative The lamp failed to ignite The processor monitors the lamp current during the ignition cycle If the lamp current does not rise above the lower limit within 2 5 s the error message is generated Probable cause 1 Lamp disconnected 2 Defective or non Agilent lamp 3 Defective power supply 4 Defective main board Suggested actions Ensure the lamp is connected Exchange the lamp Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity Il VWD User Manual No heater current Error ID 7453 Error Information 7 The lamp heater current in the detector is missing During lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within 1 the error message is generated Probable cause 1 Lamp disconnected 2 Ignition started without the top foam in place 3 Fan not running permitting lamp on 4 Defective main board 5 Defective or non Agilent lamp 6 Defective power supply Agilent 1290 Infinity II VWD User Manual Suggested actions Ensure the lamp is connected Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Exchange the lamp Please contact
128. the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1290 Infinity Il VWD User Manual In This Book In This Book This manual covers the Agilent 1290 Infinity II Variable Wavelength Detector G7114B Find information on other Agilent Variable Wavelength Detectors in separate manuals 1 Introduction to the Variable Wavelength Detector This chapter gives an introduction to the detector instrument overview and internal connectors 2 Site Requirements and Specifications This chapter gives information on environmental requirements physical and performance specifications 3 Using the Module This chapter explains the essential operational parameters of the module 4 Preparing the Module This chapter provides information on how to set up the detector for an analysis and explains the basic settings 5 Optimizing the Detector This chapter provides information on how to optimize the detector 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1290 Infinity II VWD User Manual In This Book 8 Test Functions This chap
129. the module Agilent 1290 Infinity II VWD User Manual 3 Using the Module Magnets 32 Turn on off 33 Status Indicators 34 Instrument Configuration 35 Set up the Detector with Agilent Open Lab ChemStation 37 The Detector User Interface 38 Detector Control Settings 40 Method Parameter Settings 41 Scanning with the VWWD 45 Ee Agilent Technologies 31 3 Using the Module Magnets Magnets 32 Agilent 1290 Infinity Il VWD User Manual Using the Module 3 Turn on off Turn on off Power switch 1 On 2 Off Agilent 1290 Infinity II VWD User Manual 33 3 Using the Module Status Indicators 1 The module status indicator indicates one of six possible module conditions Status indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires attention for example a leak or defective internal components 5 Resident mode blinking for example during update of main firmware 6 Bootloader mode fast blinking Try to re boot the module or try a cold start Then try a firmware update 34 Agilent 1290 Infinity Il VWD User Manual Using the Module 3 Instrument Configuration 1 Set the switches of the Configuration switch at the rear of the module a All switches DOWN module uses the default IP address 192 168 254 11 b Swi
130. ther the complete system stops As Pump Injector or the module if different from system stop time The data collection is stopped at this time A posttime period can be used to allow module s items to equilibrate e g after gradient change or temperature change Agilent 1290 Infinity Il VWD User Manual Table 4 Method Parameter Settings Agilent 1290 Infinity II VWD User Manual Using the Module Method Parameter Settings Analog Output The range can be set to either 100 mV or 1 V full scale see Table on page 40 Zero Offset 1 99 in steps of 1 5 equal to 50 mV Attenuation 0 98 4000 mAU at discrete values for either 100 mV or 1 V full scale Signal Polarity Can be switched to negative if required Autobalance Defines whether a balance is performed prior to a run and or after a run has finished Miscellaneous Lamp on required for acquisition If unchecked the lamp will be turned off after the analysis has finished Note that the lamp on requires at least one hour warm up time see Warm up of the Detector on page 69 Scan Range Step Stop Flow scan range step Access to the scan feature is only possible during run See Scanning with the VWD on page 45 Additional Signals for diagnostic purpose Sample only Reference only 3 43 3 Using the Module Method Parameter Settings Table 4 Method Parameter Settings Timetable You may set up time events to change function
131. tion 13 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 55 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 19 on page 194 Default Active Parameter Parameter Figure 56 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Agilent 1290 Infinity I VWD User Manual 193 13 LAN Configuration 194 Table 19 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connec
132. tion using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored Agilent 1290 Infinity Il VWD User Manual LAN Configuration 13 Dynamic Host Configuration Protocol DHCP General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does not offer any services for hostname resolution e g NetBIOS DHCP Active S
133. tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files Agilent 1290 Infinity Il VWD User Manual 73 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 74 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity I VWD User Manual 7 Error Information What Are Error Messages 77 General Error Messages 78 Timeout 78 Shutdown 78 Remote Timeout 79 Lost CAN Partner 80 Leak 80 Leak Sensor Open 81 Leak Sensor Short 81 Compensation Sensor Open 82 Compensation Sensor Short 82 Fan Failed 83 Open Cover 83 ERI Messages 84 Detector Error Messages 85 UV lamp no current 85 UV lamp no voltage 86 Ignition Failed 86 No heater current 87 Wavelength calibration setting failed 88 Wavelength holmium check failed 89 Grating or Filter Motor Errors 90 Wavelength test failed 91 Cutoff filter doesn t decrease the light intensity at250nm 91 ADC Hardware Error 92 Illegal Temperature Value from Sensor on Main Board 92 Illegal Temperature Value from Sensor at Air Inlet 93 Hea
134. trical Connections Rear View of the Module Configuration switch USB Mini Port t 2 z SssSsesssss Analog output gt S388 B3838 Power socket Figure 47 Rear view of detector example shows a G7114A B VWD electrical connections and label Information on Instrument Serial Number Serial Number Information 1200 Series and 1290 Infinity The serial number information on the instrument labels provide the following information CCYWWSSSSS Format CC country of manufacturing DE Germany JP Japan CN China YWW year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 SSSSS real serial number 170 Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Serial Number Information 1260 Infinity The serial number information on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany e JP Japan e CN China X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number Agilent 1290 Infinity II VWD User Manual 171 12 Hardware Information Interfaces The Agilent 1200 Infinity Series II modules provide the following interfaces Table 13 Agilent 1200 Infinity Il Series Interfaces Module CAN USB LAN RS 232 Analog APG Special on
135. trument Curves tab VWD 671148 jem Wavelength 250 nm Peakwidth gt 0 1 min 2s resp time 5 Hz Posttime Positive Negative V Lamp on required for acquisition Scan Range 190 gt to Step 2 nm Additional Signals Acquire Signal without Reference Acquire Reference only Timetable empty Figure 8 Method parameter settings NOTE For additional help and support Highlight the desired cell and press F1 A help screen will open with additional information and documentation about the topic Agilent 1290 Infinity Il VWD User Manual 41 3 Using the Module Table 4 Method Parameter Settings Signal Wavelength 250 nm Peakwidth gt 0 1 min 2 s resp time 5 Hz v lt 0 0016 min 0 016 s resp time 160 Hz Stoptime gt 0 0016 min 0 031 s resp time 160 Hz gt 0 0031 min 0 063 s resp time 160 Hz 7 gt 0 0063 min 0 13 s resp time 80 Hz OSes gt 0 013 min 0 25 s resp time 40 Hz C gt 0 025 min 0 5 s resp time 20 Hz pin gt 0 05 min 1 s resp time 10 Hz gt 0 1 min 2s resp time 5 Hz gt 0 2 min 4 s resp time 2 5 Hz gt 0 4 min 8s resp time 1 25 Hz Figure 9 G7114B Peakwidth Settings Dual Wavelength Settings V Enable Dual Wavelength Signal A 250 nm Signal B 250 nm Peakwidth gt 0 2 min 4 s resp time 2 5 Hz v gt 0 2 min 4 s resp time 2
136. ty II VWD User Manual 00 ee 4 a e Oe Preparing the Module e e e i Leak and Waste Handling 48 Waste Concept 49 Setting up an Analysis 50 Before Using the System 50 Requirements and Conditions 52 Preparing the Detector 54 Preparing the HPLC System 54 Running the Sample and Verifying the Results 56 Solvent Information 57 This chapter provides information on how to set up the detector for an analysis and explains the basic settings ee Agilent Technologies 47 4 Preparing the Module Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabin
137. uired to load resident firmware into the module e Boot Resident System Revert to Default Data The instrument will boot to resident mode and changes to the module s default parameter May be also required to load main firmware into the module Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again it will start as described above Agilent 1290 Infinity II VWD User Manual 181 12 Hardware Information Table 16 Boot Resident Forced Coldstart SW1 SW2 SW3 SW4 SW5 SW 6_Init Mode 1 0 0 0 0 0 Boot Main System Keep Data 1 1 0 0 0 0 Boot Resident System Keep Data 1 0 0 0 0 1 Boot Main System Revert to Default Data 1 1 0 0 0 1 Boot Resident System Revert to Default Data Note The setting 0 down is essential 182 Agilent 1290 Infinity Il VWD User Manual Hardware Information 12 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of
138. ume Tul Column Temperature optional 25 C or ambient Agilent 1290 Infinity Il VWD User Manual Preparing the Module 4 Typical Chromatogram A typical chromatogram for this analysis is shown in Figure 12 on page 53 The exact profile of the chromatogram will depend on the chromatographic conditions Variations in solvent quality column packing standard concentration and column temperature will all have a potential effect on peak retention and response DAD1 A Sig 254 4 Ref 500 100 04101410140003 D mAU yt a he gL a a a CC Cm CC CC E min Figure 12 Typical Chromatogram with UV detector Agilent 1290 Infinity I VWD User Manual 53 4 54 Preparing the Module Preparing the Detector For best performance of the detector Let the lamp warm up and stabilize for at least one hour initial turn on of the module requires a longer time depending on the environment and the application needs refer to Specification Conditions G7114B on page 30 For high sensitivity measurements a stable environment is required refer to Environment on page 26 Prevent drafts from air condition systems Do not work with removed open front panels doors When the system includes a G1316 TCC typically located below the detector and its front panel is removed while the TCC is set to high temperatures the up streaming air could influence the stability of the detector b
139. verview 154 Analog Cables 156 Remote Cables 158 CAN LAN Cables 162 RS 232 Cables 163 USB Cables 164 12 Hardware Information 165 Firmware Description 166 Electrical Connections 169 Interfaces 172 Setting the 6 bit Configuration Switch 179 Instrument Layout 183 Early Maintenance Feedback 184 Agilent 1290 Infinity II VWD User Manual Contents 13 LAN Configuration 187 What You Have to Do First 188 TCP IP parameter configuration 189 Configuration Switch 190 Initialization Mode Selection 191 Dynamic Host Configuration Protocol DHCP 195 Manual Configuration 198 PC and Agilent ChemStation Setup 202 14 Appendix 211 General Safety Information 212 Waste Electrical and Electronic Equipment Directive 220 Radio Interference 221 Sound Emission 222 Solvent Information 223 Declaration of Conformity for HOX2 Filter 224 Agilent Technologies on Internet 225 Agilent 1290 Infinity II VWD User Manual Agilent 1290 Infinity I VWD User Manual ee 1 r Introduction to the Variable Wavelength gt gt Detector Introduction to the Detector 10 Product Description 11 Optical System Overview 12 Flow Cell 13 Lamp 14 Source Lens Assembly 14 Entrance Slit Assembly 14 Filter Assembly 15 Mirror Assemblies M1 and M2 16 Grating Assembly 16 Beam Splitter Assembly 16 Photo Diodes Assemblies 16 Photo Diode ADC analog to digital converter 16 Dual Wavelength Mode 17 Leak and Waste Handling 18 Waste Concept 20 O
140. wo PEEK rings 2 Ring PEEK kit 3 Window quartz kit 4 Gasket kit KAPTON 5 G1314 44010 Clip for RFI ID tag 6 0515 4780 Screw for Clip M2 2 4 5 mm long 148 Agilent 1290 Infinity Il VWD User Manual Parts and Materials for Maintenance 10 High Pressure Flow Cell 10 mm 14 pL 1 Cell screw 2 Ring PEEK 3 Quartz window 4 Gasket KAPTON 5 RFID Clip 6 Screw Figure 44 High Pressure Flow Cell Agilent 1290 Infinity II VWD User Manual 149 10 Parts and Materials for Maintenance Cuvette Holder For information the use of the cuvette holder refer to Using the Cuvette Holder on page 131 p n Description G1314 60200 Cuvette Holder Figure 45 Cuvette Holder 150 Agilent 1290 Infinity Il VWD User Manual Accessory Kit Parts and Materials for Maintenance 10 Accessory kit G7114 68755 contains some accessories and tools needed for installation and repair of the module p n 5062 8535 5063 6527 5181 1516 5500 1155 5043 1013 0100 1516 Description Waste accessory kit Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste see item 4 in Figure 40 on page 140 CAN cable Agilent module to module 0 5 m Tube Connector 90 degree ID 6 4 Tubing Clip see item 7 in Figure 40 on page 140 Fitting male PEEK 2 pk Agilent 1290 Infinity II VWD User Manual 151 10 Parts and Materials for Maintenance Accessory Kit 152 Agilent 1290 Infinity Il VWD User Manual Agilent 1290 Infinity II
141. xternal circuits before removing the instrument cover Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained In Case of Damage WARNING Damage to the module Personal injury for example electrical shock intoxication gt Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel 214 Agilent 1290 Infinity Il VWD User Manual Appendix 14 Solvents WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for th

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