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1. 32 Channel Miniature Preamplifier for FlexMEAs MPAI32 FLEX User Manual Service and Maintenance Cleaning the Connectors Warning Make sure that only the connectors touch the liquid do not submerge the miniature preamplifier or the cable into cleaning solutions Otherwise you can fatally damage the electronics It is recommended to avoid the use of aqueous cleaning solutions to avoid corrosion If necessary use distilled water Clean the connectors with 70 alcohol and cotton swabs from time to time If this does not provide satisfying results insert only the connectors into a small beaker with acetone or alcohol and sonificate for 10 20 s in an ultrasonic bath Air dry the miniature preamplifier for about 5 min before use You can clean the connectors with distilled water in an ultra sonic bath Dry the connectors with compressed air immediately after sonification and let the amplifier air dry for at least 6 h before use Sterilization Warning Do not autoclave or sterilize miniature preamplifiers by high heat above 70 C or vapor The resin is not heat stable and may deform under heat Vapor can lead to a corrosion of the electronics Miniature preamplifiers can be sterilized with standard methods that are not based on high heat or vapor for example with 70 ethanol UV light or by thermal sterilization in an oven at 56 C with an incubation time of 8 hours 5 Pin Layout 5 1 Power Supply Pin Layout Supply v
2. in accordance with recognized safety engineering rules The device may only be used for its intended purpose be used when in a perfect condition Improper use could lead to serious even fatal injuries to the user or third parties and damage to the device itself or other material damage Warning The device and the software are not intended for medical uses and must not be used on humans Malfunctions which could impair safety should be rectified immediately Any physical damage of the cable such as a broken cable causes a physical damage of the miniature preamplifier that cannot be repaired Protect the device from heat Do not autoclave You can clean the connectors with distilled water in an ultra sonic bath but keep the cable away from the fluid Dry the device with compressed air Use and keep the device always in a dry environment Do not expose it to fluids or vapor for a longer period of time Welcome to the MPA32I FLEX 2 Welcome to the MPA32I FLEX The 32 channel Miniature Preamplifier MPA32I is connected to the microelectrodes for providing the initial tenfold amplification stage It has additional common ground and reference electrode inputs The reference electrode is ideally identical to the recording electrodes and placed into a comparable but inactive area or tissue Background or noise signals that are picked up by both the reference electrode and the recording electrodes are removed The metal ca
3. C 3Vto 8VDC 3Vto 8VDC 3Vto 8VDC Supply current lt 2 MA typically 1 mA lt 6 mA typically 4 mA lt 14 MA typically 9 mA lt 14 mA typically 9 mA Number of input channels 2 8 32 32 Input voltage 0 to 500 mV with respect to a supply 0 to 500 mV with respect to a supply 0 to 500 mV with respect to a supply 0 to 500 mV with respect to a supply voltage of 5 V voltage of 5 V voltage of 5 V voltage of 5 V Input impedance 10 Q parallel to 10 pF 10 Q parallel to 10 pF 10 Q parallel to 10 pF 10 Q parallel to 10 pF Input noise lt 1 5 UV rms 1 Hz to 5 kHz inputs short lt 1 5 UV as 1 Hz to 5 kHz inputs short lt 1 5 UV aus 1 Hz to 5 kHz inputs short lt 1 5 UV pms 1 Hz to 5 kHz inputs short circuited circuited circuited circuited Noise density e 15 nV IH en 15 nV IH en 15 nV JHz en 15 nV NHz Number of output channels 2 8 32 32 Output voltage supply voltage supply voltage supply voltage supply voltage Output current max 10 mA max 10 mA max 10 mA max 10 mA Output impedance oQ 00 oQ 00 Bandwidth DC to 50 kHz DC to 50 kHz DC to 50 kHz DC to 50 kHz Gain 10 10 10 10 Versions with 2 8 and 32 channels available MPA2I MPA8l and MPA32I respectively MPA 32IFLEX is a special version for use with Flex MEAs Page 1 of 1 O 2003 Multi Channel Systems MCS GmbH multichannel FlexMEA systems 36 Electrodes 32 recording electrodes 2 indifferent reference electrodes 2 large groun
4. d electrodes for use with miniature preamplifier MPA32I FLEX 9 09 909090 9000 The letter digit code is the electrode identifier and refers to the position of the electrode in the grid Below are the recording channel numbers in italics that refer to the channel numbers in the MC_Rack program Please make sure that you have selected the linear channel layout in the MC_Rack program See the MC_Rack user manual or help for details If you use more than one MPA32I FLEX and a ME64 System the signal collector SC2x32 leads the output channels of the second amplifier to channel number 33 64 Please see the SC2x32 data sheet for details Multi Channel Systems Fon 49 7121 9 09 25 O O 2002 2005 Multi Channel Systems MCS GmbH Fax 49 7121 9 09 25 11 MCS GmbH Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Germany www multichannelsystems com Product information is subject to change without notice
5. eamplifier with 2 MPA2I Small sized and light weight electrode inputs headstage with common ground Miniature preamplifier with 8 MPA8I ano acai Tonal indifferent reference a electrode input input type I gain electrode inputs 10 Miniature preamplifier with MPA32I 32 electrode inputs Miniature preamplifier with MPA32IFLEX 32 electrode inputs for use with FlexMEAs Filter amplifiers with 4 8 16 FANNX NN is the total number of channels X 32 48 or 64 channels and is the input type S or I with custom input type S or gain and bandwidth Amplifier with programmable PGA16 Gain programmable from 10 to 5000 gain 16 channels with custom bandwidth Amplifier with programmable PGA32 gain 32 channels Amplifier with programmable PGA64 gain 64 channels Amplifier with programmable PGA1632 Gain programmable from 10 to 5000 gain 16 input and 32 output with two different custom pass bands channels Amplifier with programmable PGA3264 gain 32 input and 64 output channels 11 32 Channel Miniature Preamplifier for FlexMEAs MPAI32 FLEX User Manual 7 3 Accessories Signal collector for 2x MPA32I SC2x32 Collects the channels from each of the up to 2 miniature preamplifiers MPA32I and leads them to the amplifier via a 68 pin MCS standard cable Power supply with 20 W For supplying power to power and 7 V output programmable gain amplifiers for voltage custom setups that lack an internal power
6. f the black with your fingers and carefully pull open the slot cover only a little bit about 1 or 2 mm Do not demount the cover as the plastic supports might break easily 1 2 3 Insert the FlexMEA into the slot with the contact pads upside down Carefully push back the slot cover until it locks 3 3 FlexMEAs FlexMEAs FlexMEAs are made of flexible polyimid material perfect for in vivo and special in vitro applications Only 12 um thick and weighing less than 1 g the FlexMEA biosensor is very thin and lightweight FlexMEAs have 32 electrodes plus two indifferential reference electrodes and two ground electrodes More layouts can be provided on request The flexible base is perforated for a better contact with the surrounding tissue The electrodes have a diameter of about 30 um with an interelectrode distance of 300 um Conducting material is pure gold Using FlexMEAs FlexMEAs are usually connected to a headstage preamplifier that is connected to a filter amplifier or programmable gain amplifier see also the ME System product line of Multi Channel Systems FlexMEAs can be directly connected to a 32 channel miniature preamplifier from Multi Channel Systems for in vivo experiments FlexMEAs are stable at a temperature range from 10 C to 40 C Warning Do not autoclave or sterilize FlexMEAs by heat These MEA types are not heat stable and will be irreversibly damaged if the temperature is too high
7. ible to avoid noise pickup As the total amplifier gain generally lies in the range of 1000 even very small noise signals may generate high noise signals If you are not using a Faraday cage it might be necessary to shield the complete setup with aluminum foil or similar to prevent electrical interference from the outside Connect the aluminum foil to the ground of the setup for example the metal table 3 2 2 3 32 Channel Miniature Preamplifier for FlexMEAs MPAI32 FLEX User Manual Troubleshooting If you observe problems with noise check that the ground of the setup is connected to exactly the same ground as the data acquisition computer For example connect the metal table to the ground earth wire of a free power outlet of the same electrical system as the computer is generally connected to ground earth via the power plug too Also connect the data acquisition computer s metal case with a thick ground wire to the ground of the setup for example the metal table preferably with a 4 mm plug Connecting FlexMEAs a The MPA32I FLEX has a slot for inserting FlexMEAs For a tight and reliable connection the FlexMEA contacts are pressed down onto the amplifier contacts by the slot lid To insert the MEA you have to pull open the slot lid Inserting the Flex MEA into the MPA32I FLEX slot E Pull open the slot cover about 2 mm A E Insert _ GD Push back the slot cover until it locks as Grip the sides o
8. in 19 Channel 18 Channel 19 Channel 20 Channel 21 Channel 22 Channel 23 Channel 24 Channel 25 Channel 26 Channel 27 Channel 28 Channel 29 Channel 30 Channel 31 Channel 32 GNDP power ground Positive supply voltage Negative supply voltage Connected to the ground of the amplifier The signal ground is used as the reference for the following filter amplifier 32 Channel Miniature Preamplifier for FlexMEAs MPAI32 FLEX User Manual Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site User forum The Multi Channel Systems User Forum provides an excellent opportunity for you to exchange your experience or thoughts with other users worldwide Mailing list If you have subscribed to the ME System mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the list on the MCS web site www multichannelsystems com 10 Ordering Information 7 Ordering Information Please contact your local retailer for pricing and ordering information 7 1 ME Systems Data acquisition system ME16 System Complete with data acquisition IPS10W and software package Data acquisition system ME32 System with 32 analog channels Data acquisition system ME64 System with 64 analog channels Data acquisition system ME128 System with 128 analog channels 7 2 Amplifiers Miniature pr
9. multichannel systems 32 Channel Miniature Preamplifier for FlexMEAs MPA32I FLEX User Manual ME Information in this document is subject to change without notice No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH While every precaution has been taken in the preparation of this document the publisher and the author assume no responsibility for errors or omissions or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document 2002 2005 Multi Channel Systems MCS GmbH All rights reserved Printed 2005 10 21 Multi Channel Systems MCS GmbH Aspenhaustra e 21 72770 Reutlingen Germany Fon 49 71 21 90 925 0 Fax 49 71 21 90 92 5 11 info multichannelsystems com www multichannelsystems com 1 1 1 2 1 3 3 1 3 2 3 3 4 1 4 2 5 1 5 2 5 3 7 1 7 2 7 3 7 4 Table of Contents Important Information and Instruction Operator s Obligations Guaranty and Liability Important Safety Advice Welcome to the MPA32I FLEX Setting Up and Connecting the MPA General Setup Recommendations Connecting FlexMEAs FlexMEAs Service and Maintenance Cleaning the Co
10. nnectors Sterilization Pin Layout Power Supply Inputs Outputs Contact Information Ordering Information ME Systems Amplifiers Accessories FlexMEAs Technical Specifications no u ui Uy N gt gt 0 WO Oo o O 10 11 11 11 12 12 1 1 1 2 Important Information and Instruction Important Information and Instruction Operator s Obligations The operator is obliged to allow only persons to work on the device who are familiar with the safety at work and accident prevention regulations and have been instructed how to use the device are professionally qualified or have specialist knowledge and training and have received instruction in the use of the device have read and understood the chapter on safety and the warning instructions in this manual and confirmed this with their signature It must be monitored at regular intervals that the operating personnel are working safely Personnel still undergoing training may only work on the device under the supervision of an experienced person Guaranty and Liability The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply The operator will receive these no later than on conclusion of the contract Multi Channel Systems MCS GmbH makes no guaranty as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the
11. oltage is applied to the output connector pins 19 and 37 The voltage source should supply a stable noise free voltage Do not exceed the maximum voltage Pin 19 3 V to 8 V Pin 37 3 V to 8 V Warning Do not mismatch the polarity of the power supply A false connection may damage the unit 5 2 Inputs 32 Channel Miniature Preamplifier for Flex MEAs inputs 00000000000000000009099595909990090090909995 Pin 1 Pin 2 Pin 3 34 Pin 35 Pin 36 GND ground Reference Channel 1 32 Reference GND ground This illustration shows the pin layout viewed from the front with the case screws upside down The two reference inputs are used for connecting a reference electrode Both inputs are equal so it does not matter which of the two inputs is used If both are used the mean of both reference signals is used as reference 5 3 Outputs 32 Channel Miniature Preamplifier outputs 0000 MOOO ASES OB Pin 1 GNDP power ground Pin 20 GNDS signal ground Pin 2 Channel 1 Pin 21 Channel 2 Pin 3 Channel 3 Pin 22 Channel 4 Pin 4 Channel 5 Pin 23 Channel 6 Pin 5 Channel 7 Pin 24 Channel 8 Pin 6 Channel 9 Pin 25 Channel 10 Pin 7 Channel 11 Pin 26 Channel 12 Pin 8 Channel 13 Pin 27 Channel 14 Pin 9 Channel 15 Pin 28 Channel 16 Pin 10 Channel 17 Pin 29 Pin 11 Pin 30 Pin 12 Pin 31 Pin 13 Pin 32 Pin 14 Pin 33 Pin 15 Pin 34 Pin 16 Pin 35 Pin 17 Pin 36 Pin 18 Pin 37 P
12. r heart for example at the belly Connect the GND input or the metal case of the miniature preamplifier to the common ground of the setup The GND input is internally connected to the metal case Connect the reference electrode to the reference input of the miniature preamplifier Generally a reference electrode is inserted into non active tissue of the experimental model The reference electrode should be identical to the recording electrode so that both electrodes see the same background noise This is necessary because despite the grounding the animal s body often has not exactly a potential of zero due to the electrode impedance for example The background noise is then subtracted from the recording signal increasing the signal to noise ratio Please note that this may not work if the complete setup is not properly grounded Optional If two reference electrodes are used connect the second electrode to the free reference input Otherwise leave it free It is generally not necessary to use a second reference electrode It can be used to enlarge the surface area of the reference though Connect the recording electrodes to the recording channels of the miniature preamplifier You can either use single electrodes or multitrodes with the miniature preamplifier MCS provides adapters for standard probes Connect all unused recording channels to the REF input or to the reference electrode or to the amplifier s ground if that is not poss
13. ry thin and lightweight The flexible base is perforated for a better contact with the surrounding tissue 3 1 Setting Up and Connecting the MPA Setting Up and Connecting the MPA General Setup Recommendations In the following you find general recommendations for the installation Miniature preamplifier setup Pin assignment gt Reference input Always connect to reference electrode Recording electrodes MPA32I gt Recording inputs Connect all un used recording channels to REF gt GND Ground Connect GND to ground of experimental setup Metal table ground of setup Important It is important that the complete setup refers to a single common ground The reference input has always to be connected It is recommended to use a reference electrode However if you are not using a reference electrode connect the reference input to ground GND Otherwise noise picked up by the reference input will be subtracted from the recording signals This will either lead to signal loss or to a very high noise level Ground the animal with a ground electrode of large surface area for example a liquid gel adhesive electrode that is connected to the ground of the setup for example a large metal table or a Faraday cage to avoid pickup of noise from the environment The ground electrode is best positioned in an electrically inactive region not near muscle no
14. se provides electrical shielding Electrode damage is prevented by the very low bias current The high input impedance ensures stable long term recordings Ideally the input impedance would be infinite As low voltages are generally recorded a high current would flow if the input impedance were low As a result the amplifier would not be able to deliver the current and the voltage would break down The miniature preamplifier has a high input impedance to avoid this problem The MPA32I Flex is optimized for the use with flexible microelectrode arrays FlexMEAs from Multi Channel Systems FlexMEAs are two dimensional microelectrode arrays with 32 microelectrodes for recording or stimulation 2 indifferent reference electrodes and 2 large ground electrodes They are intended for recordings from several species in both acute and long term chronic experiments Please see the MEA User Manual for more details FlexMEA electrodes are planar with a diameter of only 30 pm resulting in an impedance of about 50 kilohms arranged in a square 6 x 6 grid with an interelectrode distance of 300 um FlexMEAs are made of flexible and biocompatible polyimid as the base material supporting the titanium nitride TiN electrodes and the gold thin film conductors TiN allows the formation of electrodes as small as possible with an excellent signal to noise ratio and a long lifetime Only 12 um thick and weighing less than 1 g the FlexMEA biosensor is ve
15. supply or for other custom applications 7 4 FlexMEAs Flexible microelectrode array FlexMEA With 36 electrodes in total FlexMEA 32 electrodes arranged in a 6 x 6 grid 2 reference electrodes 2 ground electrodes 30 um electrode diameter 300 um interelectrode distance TiN electrodes and gold tracks embedded in flexible polyimid 12 Technical Specifications Date of Print 23 11 2005 MPA Miniature Preamplifier multicha n nel ale systems MPA2I MPA3I MPA32I MPA32IFLEX Operating temperature 0 C to 50 C 0 C to 50 C 0 C to 50 C 0 C to 50 C Storage temperature 0 C to 50 C 0 C to 50 C 0 C to 50 C 0 C to 50 C Relative humidity 10 to 85 non condensing 10 to 85 non condensing 10 to 85 non condensing 10 to 85 non condensing Dimensions W x D x H 12 mm x 31 mm x 3 mm 17 mm x 25 mm x 1 8 mm 27 mm x 36 mm x 5 mm 27 mm x 34 mm x 5 mm Weight 1 3 g w o cable and plug 21 g with cable 7 g w o cable and plug 56 g with cable 7 g w o cable and plug 56 g with cable and plug and plug and plug Maximum tensile strength of cable 2kg 2kg 2kg 2kg Input connector type Single row precision sockets 50 mil 1 27 Single row precision sockets 50 mil 1 27 Dual row precision sockets 50 mil 1 27 Special input for use with FlexMEAs mm grid pattern for 0 35 0 45 mm mm grid pattern for 0 35 0 45 mm mm grid pattern for 0 35 0 45 mm round pins round pins round pins Supply voltage 3Vto 8VD
16. validity of his findings Guaranty and liability claims in the event of injury or material damage are excluded when they are the result of one of the following Improper use of the device Improper installation commissioning operation or maintenance of the device Operating the device when the safety and protective devices are defective and or inoperable Non observance of the instructions in the manual with regard to transport storage installation commissioning operation or maintenance of the device Unauthorized structural alterations to the device Unauthorized modifications to the system settings Inadequate monitoring of device components subject to wear Improperly executed and unauthorized repairs Unauthorized opening of the device or its components Catastrophic events due to the effect of foreign bodies or acts of God 32 Channel Miniature Preamplifier for FlexMEAs MPAI32 FLEX User Manual Important Safety Advice Warning Make sure to read the following advices prior to install or to use the device and the software If you do not fulfill all requirements stated below this may lead to malfunctions or breakage of connected hardware or even fatal injuries Warning Obey always the rules of local regulations and laws Only qualified personnel should be allowed to perform laboratory work Work according to good laboratory practice to obtain best results and to minimize risks The product has been built to the state of the art and

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