autoMACS® Pro Separator
Contents
1. forthe isolation of cells with frequencies higher than 596 and normal antigen expression if recovery is the highest priority Posseld Positive selection in standard mode double column program for the isolation of cells with frequencies lower than 5 and normal antigen expression in a small elution volume Posseld2 Positive selection in standard mode Il double column program for the isolation of cells with frequencies lower than 5 and normal antigen expression if purity is the highest priority Short instructions Note When using the program Posselwb the whole blood sample will be diluted 3 fold the starting volume Posselds Positive selection in sensitive mode double column program for the isolation of cells with frequencies lower than 5 and low antigen expression Posselwb Special positive selection in special mode double column program forthe isolation of cell subsets from whole blood cell samples are automatically diluted with Running Buffer Depletion programs Deplete Depletion in standard mode for removal of cells with normal antigen expression if recovery is the highest priority for untouched isolation with MACS Cell Isolation Kits Depletes Depletion in sensitive mode I for removal of cells with normal antigen expression if purity is the highest priority for removal of cells with low antigen expression for untouched isolation with MACS Cell Isolat2. o ES m HN ry E Be mm Figure 4 1 Specifications of a chill rack shown here a Chill 5 Rack and MACS Reagent Rack 4 R1 R4 correspond to the reagent rack positions Row A contains the tubes for original samples i e Ori Row B contains tubes for negative unlabeled fractions i e Neg Row C contains tubes for the positive magnetically labeled fractions i e Pos Short instructions Rack type Slots Maximal number Manual labeling Autolabeling and symbol of samples Maximal Minimal first sample volume incubation volume Chill 5 24x5 mL 6 2 5 mL 0 2 mL 5 mL tubes 0 25 mL Chill 15 15x15mL 5 12 5 mL 0 2 mL 5x5 mL 15 mL tubes 1m Chill 50 6x50mL 3 50 mL 4 m 3x15 mL 50 mL tubes 3x5 mL Volumes refer to whole blood samles only Table 4 1 MACS Chill Rack specifications for manual labeling and autolabeling For further details on sample volumes for autolabeling refer to table 3 1 Short instructions Maximal final labeling volume 2 0 mL 1 mL 6 5 mL 4mU 8mL 17 18 5 Labeling Cells can be labeled with MACS MicroBeads either manually or using the autolabeling function of the autoMACS Pro Separator For detailed information on manual labeling please refer to the Cell Separation Reagent data sheet For a list of Cell Separation Reagents optimized for autolabeling please contact Technical Support For autolabeling insert the MACS Reagent Rack 4 ont
3. 6 1 Cell separation after autolabeling 1 Highlight the desired position s in the sample separation template 2 Assign an autolabeling program from the Labeling submenu to the respective position S 09 55 Figure 6 2 The Cell Separation Reagent CD4 MicroBeads human was assigned to rack positions 1 and 2 The separation program Possel and wash program Qrinse were automatically selected Short instructions 3 Optional The recommended cell separation and wash program will be automatically displayed after choosing the autolabeling program It is possible to change the separation program or the wash program between samples or to assign the Sleep program after finishing the last sample Highlight the desired cell separation and wash program in the Separation and Wash submenus respectively 4 Insert a sample volume in the Volume submenu using the numeric keypad Select Enter For detailed information on sample volumes refer to table 3 1 Figure 6 3 A sample volume of 160 uL was entered in the Volume submenu 5 Select Run to start the cell separation Select Ok to confirm that enough buffer is available for processing all samples E Figure 6 4 A cell separation using CD4 MicroBeads human will be performed on sample positions 1 and 2 After processing sample 2 the Sleep program will be performed as a final wash step before the instrument goes into sleep mode Clicking Run will start the experiment
4. 7 v 2 o 2 o TD o fe E z e 3 El E E E o o o E e iS gt y 5 3 S S 5 5 POS 8 2 g amp 6 a Wi 9 ED n ED ov EO o M 2 7 7 os 3 a bl as a i del ui 5 o o E v vs E 3 amp E 3 oe Er E a uno a NG Ds 50 SEO n leas as as A 8 5 2 O5 u s mi Az eA ee perros IO Pons na oS ES wo wo Wy Wo Wy LU oz mp LU 5 W S n gt n gt ns ns ns ns I EE am i m ae a az a 25 EE Go e s o os v v ae amp amp aa aa amp at ag aa aa Purity will slightly decrease Recovery will slightly decrease Short Instructions Miltenyi Biotec Germany Austria Switzerland Miltenyi Biotec GmbH Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany General information Phone 49 2204 8306 0 Fax 49 2204 85197 macs miltenyibiotec de Orders Phone 49 2204 8306 20 Fax 49 2204 8306 235 macsorders miltenyibiotec de USA Canada Miltenyi Biotec Inc 2303 Lindbergh Street Auburn CA 95602 USA Phone 800 FOR MACS Phone 1 530 888 8871 Fax 1 530 888 8925 macs miltenyibiotec com www miltenyibiotec com Australia Miltenyi Biotec Australia Pty Ltd Unit 16A 2 Eden Park Drive North Ryde NSW 2113 Australia Phone 61 2 8877 7400 Fax 61 2 9889 5044 macs miltenyibiotec com au Benelux Miltenyi Biotec B V Schipholweg 68 H 2316 Leiden The Netherlands macs miltenyibiotec nl Customer service Netherlands Phone 0800 4020120 Fax 0800 4020100 Custome
5. Miltenyi Biotec autoMACS Pro Separator Short instructions Copyright 2011 Miltenyi Biotec All rights reserved No part of this user manual may be reproduced stored in a retrieval system transmitted published or distributed in any form or by any means electronically mechanically by photocopying microfilming recording or otherwise without the prior written consent of Miltenyi Biotec however notwithstanding the foregoing the owners ofthe autoMACS Pro Separator may make copies solely for purposes of training personnel in the use and servicing of the unit within their business or organization Maximal care has been taken by Miltenyi Biotec in the preparation of this user manual However Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this document The information in this document is provided as is without warranty of any kind and is subject to change without notice autoMACS MACS and the MACS logo are registered trademarks or trademarks of Miltenyi Biotec GmbH or its affiliates in Germany the United States and or other countries All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only Unless otherwise specifically indicated Miltenyi Biotec products and services are for research use
6. Short instructions 23 24 Note If the Clean program has been enabled it will also appear in the Wash submenu 6 2 Cell separation after manual labeling 1 Highlight the desired position s in the sample separation template gl Figure 6 5 Select multiple sample positions in order to set them up simultaneously 2 Select from the Labeling submenu for manual labeling 3 Optional It is not mandatory to assign a volume to manually labeled samples However the autoMACS Pro Separator requires this information to calculate and display the total sample processing time Unless otherwise indicated it is recommended to dilute manually labeled samples to a volume of 500 uL per 10 cells For detailed information please refer to the corresponding data sheet Enter the sample volume in the Volume submenu using the numeric keypad Select Enter Figure 6 6 A volume of 500 uL was assigned to samples 1 and 2 4 Assigna separation program and a washing program to each sample position The selected programs will be displayed in the programming field For details refer to section 1 1 Short instructions 5 Select Run to start the cell separation Select Ok to confirm that enough buffer is available for processing all samples CE Figure 6 7 The separation program Possel will be performed on sample positions 1 and 2 After processing sample 2 the Sleep program will be performed as a final wash step before the instru
7. 5 Remove column from slot unscrew top column connector followed by the bottom column connector as shown in figure 7 2 6 Dispose ofthe expired column 7 Pointthe bottom of the fresh column towards the autoMACS Pro Separator 8 Insert bottom column connector Screw in the column by turning it clockwise Repeat the procedure for the top column connector 1 Top Column Connector 2 autoMACS Column 3 Bottom Column Connector Select column ection 14 42 Figure 7 2 Top Exchange of the column Bottom Starting the Col ex program 9 Pushcolumn into the magnet housing with the top column connector sitting on the guide in the column slot 10 Repeat installation for the second autoMACS Column 11 After exchange of separation columns select Done The autoMACS Pro Separator system will be automatically primed with Running Buffer and is then ready for cell separation Short Instructions 8 Decision tree for the optimal separation program Frequency of target cells d Depletion Depletion H e gt 5 to 10 lt 5 to 10 using MACS ate A MieroBeads Cell Isolation Kit Level of Level of Level of Purity is antigen antigen antigen highest expression expression expression priority Normal to Normal to Normal to high Ow high Low Low high Recovery Purity is Recovery is highest highest is highest priority priority priority i 4 i v o a o v E z E E 3 3 2 Z o Es a eo E 5 g o o
8. Sleep It is mandatory to use Sleep as the last wash program before overnight storage This program uses Washing Solution and storage solution Upon completion of the Sleep program the fluidic system contains 7096 ethanol 1 2 Choose appropriate tube rack Select the tube rack according to the desired number of samples number of cells and sample volume refer to table 4 1 and ensure that it is pre cooled to 4 C 1 3 Prepare cell samples Prepare a single cell suspension and avoid cell aggregates e g using Pre Separation Filters 30 um 130 041 407 or Pre Separation Filters 70 um 130 095 823 Remove dead cells e g using the Dead Cell Removal Kit 130 090 101 Short instructions 10 2 Setting up and priming the autoMACS Pro Separator Note The connectors for the fluid bottles are color coded blue for Running Buffer green for Washing Solution black for storage solution and red for the waste bottle 2 1 Setup of the instrument 1 Check that all bottles are filled with the appropriate solutions and connected to the appropriate sensor cables Empty the waste bottle Green sensor cable for Washing Solution Blue sensor cable for Running Buffer Bottle closure Figure 2 1 Bottle closures and sensor cables of the fluid bottles 2 Check that the MACS MiniSampler the 2D code reader and the fluid sensor cables are attached correctly to the back of the instrument Sensor cable plug 2
9. Untouched 2 107 cells 160 uL 4x10 5200 uL 1 3x10 isolation per 40 uL Cell Isolation Kits Untouched 3 107 cells 120 uL 4x10 5850 uL 1 3x10 isolation per 30 uL MicroBead Kits Positive 2 10 cells 120 pL 2x10 5850 uL 6 5x10 selection or per 60 uL depletion Chill 50 Rack Whole Blood Whole blood or 1 Original AmL 8mL MicroBeads bone marrow volume 1 Max number of samples 6 min first incubation volume 0 2 mL max final labeling volume 2 mL Max number of samples 5 min first incubation volume 0 2 mL max final labeling volume 6 5 mL Max number of samples 3 min first incubation volume 4 mL max final labeling volume 8 mL When working with fewer cells than the necessary minimal volume resuspend cells in the stipulated minimal volume Table 3 1 Dilution volumes for the first labeling step and MACS Chill Rack specifications for autolabeling including minimal and maximal volumes and cell numbers Short instructions 16 4 Select the appropriate tube rack MACS Chill Racks are automatically detected by the autoMACS Pro Separator Three different tube racks are available for processing sample volumes between 0 2 mL and 50 mL 1 Select the appropriate tube rack according to table 4 1 2 Cool down the tube rack for 3 4 hours in a refrigerator 2 8 C or until the coolant becomes solid Do not cool below 0 C as samples may freeze 3 Equipthe tube rack with sample tubes and fraction collection tubes
10. position R1 was selected for reagent assignment 2 Select Enter Reagent from the lower navigation bar Enter the reagent specific product order number The product order number is located on the Cell Separation Reagent data sheet If this is not available visit the respective product page at www miltenyibiotec com to download a printable PDF of the document Short instructions 3 Ifa correct product number was entered the software will immediately recognize and list the reagent or kit components To confirm your choice select the listed reagent by using the touch screen M The next available rack position will be automatically highlighted Repeat the procedure for the remaining reagents or kit components i Ame Cut A Coda ma Lamm het Modan TE CIE Figure 5 6 Entry of the components of the NK Cell Isolation Kit Il mouse The reagent NK Cell Biotin Antibody Cocktail mouse was assigned to rack position R1 The reagent Anti Biotin MicroBeads was assigned to rack position R2 4 Select Okto complete the reagent entry Short instructions 21 22 6 Cell separation Place the appropriate Chill Rack onto the MACS MiniSampler For details refer to table 4 1 Go to the Separation menu to set up the sample rack template H Sample rack template IZ Sample labeling options 3 Sample processing volume 4 Separation program 5 Wash procedure u Figure 6 1 Sample rack template in the Separation menu
11. D code reader plug MACS MiniSampler plug Power socket Figure 2 2 Plugs of accessories attached to the back of the instrument Short instructions 3 Switch ON the autoMACS Pro Separator After initialization of the instrument the touchscreen displays the Status menu ON OFF switch Figure 2 3 Location ofthe ON OFF switch 2 2 Priming of the instrument To prime the instrument go to the Separation menu 1 Selectthe Separation menu 2 Select Wash Now from the lower navigation bar 3 Select Rinse and Run 2 3 Monitoring the instrument status prior to cell separation The instrument status can be determined by viewing the Status menu at any time El Sample status IZ Fluid bottle filling status 3 Column status I Tube rack status I5 MACS MiniSampler status DRE 17 00 Figure 2 4 Overview of the Status menu Short instructions 11 12 2 3 1 Status of fluid bottles Confirm that the fluid bottle status is ready Figure 2 5 Left Fluid bottles are shown in green indicating ready Right Fluid bottles are shown in red and need to be replaced Bottle Symbol Symbol color and user action Running Buffer Green no action required Red refill bottle Gray connect bottle sensor Washing Solution Green no action required Red refill bottle Gray connect bottle sensor Storage solution Gray no liquid detection visually check volume Waste Green no action required Red empty waste or
12. arrow system with storage applications solution Washing Solution and Running Buffer Sleep Rinse with Washing Before switching Solution followed by OFF the autoMACS filling with storage Pro Separator solution Table 7 2 Specifications of programs for daily maintenance Short Instructions Duration 1 5 min 4 min Duration 4 min 7 min 5 min 7 3 Periodic maintenance Action Description Recommended Duration usage Column Replacement of Every two weeks 6 min exchange separation columns OR after 100 using the separations Col ex whichever comes program first Running Decontamination Every 3 6 months 21min the Safe procedure with MACS program Bleach Solution Cleaning Cleaning of pump syringe Every 1 3 months thepump referto user manual syringe Running Rinse with Washing Before storing the the Store Solution followed by instrument for a program storage solution period longer than replacement of columns two weeks with substitutes Table 7 3 Specifications of programs for periodic maintenance 7 4 Column exchange Replace autoMACS Columns every two weeks or after 100 separations whichever comes first 1 Gotothe Option menu Select Special and Col ex 2 Press Run 3 When prompted exchange the columns Figure 7 1 Col ex program Short Instructions 29 30 4 Open front door and note the positions of the columns column 1 left column 2 right Exchange one column at a time
13. cifically indicated Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use autoMACS and MACS are registered trademarks of Miltenyi Biotec GmbH Copyright 2011 Miltenyi Biotec GmbH All rights reserved 140 002 015 05
14. d Error Check screen for error detection Purple Program Sleep is Switch OFF autoMACS completed Pro Separator Blinking Action required Check screen for required action Table 6 2 Fluid bottle illumination 6 4 Setting the autoMACS Pro Separator in sleep mode 1 amp Press the shutdown symbol upper right hand corner of the display 2 Alternatively select Sleep program as the last washing step 6 5 Optional Switch OFF the instrument for long term storage 1 Goto the Option menu and select Special 2 Select Store and press Run 3 Replace the columns with column substitutes refer to section 3 3 4 of the user manual 4 Select Done 5 Switch OFF the autoMACS Pro Separator using the main power switch Short instructions 27 28 7 Maintenance 71 Rinsing programs Program Description Recommended usage Qrinse Standard short rinse of Between separation columns and separations of tubing system with frequent cells Running Buffer gt 5 96 Rinse Rinse of separation Between and columns and tubing before separations system with Washing of rare cells Solution and Running lt 5 Buffer Table 7 1 Specifications of the rinsing programs 7 2 Daily maintenance programs Program Description Recommended usage Rinse Rinse of separation Prior to first columns and tubing separation system with Washing Solution and Running Buffer Clean Rinse of separation After whole blood columns and tubing and bone m
15. edures Minimal and maximal volumes and total cell numbers in table 4 1 account for autolabeling samples only For a current list of Cell Separation Reagents and Kits that are optimized for cell separations with the autoMACS Pro Separator autolabeling feature please contact Mitenyi Biotec Technical Support Short instructions Cell Separation Strategy Number of Dilution autolabeling Reagani meges TAME Minimal Minimal Maximal Maximal volume totalcell volume total cell number number Chill 5 Rack Direct MicroBeads Positive 1 107 cells 160 uL 2x10 1600 uL 2x10 human rat selection or per 80 uL non human primate depletion Direct MicroBeads Positive 1 10 cells 180 uL 2x10 1800 uL 2x10 mouse selection or per 90 uL depletion Whole Blood Whole blood or 1 Original 0 25 mL 1mL MicroBeads bone marrow volume Cell Isolation Kits Untouched 2 107 cells 160uL 4x10 1600 uL 4x10 isolation per 40 uL Cell Isolation Kits Untouched 3 107 cells 120 pL 4x10 1200 uL 4x10 isolation per 30 uL MicroBead Kits Positive 2 107 cells 120 pL 2x107 1200 uL 2x10 selection or per 60 uL depletion Chill 15 Rack Direct MicroBeads Positive 1 107 cells 160 uL 2x10 5200 uL 6 5x10 human rat selection or per 80 uL non human primate depletion Direct MicroBeads Positive 1 107 cells 180 uL 2x10 5850 uL 6 5x10 mouse selection or per 90 uL depletion Whole Blood Whole blood or 1 Original 1mL 4mL MicroBeads bone marrow volume Cell Isolation Kits
16. instructions 1 Considerations before you start WARNING Please read the user manual of this instrument carefully and pay attention to all warnings and precautions 1 1 autoMACS Pro cell separation and wash programs There are two basic strategies for separating specific cell populations positive selection and depletion During positive selection the target cells are magnetically labeled and collected as the positive fraction During depletion the unwanted cells are labeled and depleted from the target cells The target cells are collected as the negative fraction Furthermore sequential sorting allows the performance of two consecutive separations For more information please refer to www macscellseparation com The autoMACS Pro Separator provides a selection of twelve pre set separation programs The appropriate program is generally chosen depending on the separation strategy the target cell frequency and the level of antigen expression A decision tree on what cell separation program to choose is located in section 8 of this document For further information please refer to the respective Cell Separation Reagent data sheet Positive selection programs Possel Positive selection in standard mode for the isolation of cells with frequencies higher than 5 and normal antigen expression Possel s Positive selection in sensitive mode forthe isolation of cells with frequencies higher than 596 and low antigen expression
17. ion Kits if purity is highest priority Depl05 Depletion in sensitive mode Il for removal of cells with low antigen expression special program for very sensitive depletion Depl025 Depletion in sensitive mode Ill for removal of cells with low antigen expression special program for very sensitive depletion A_DepI07 Depletion in standard mode via loading of sample in separate 1 mL stages for removal of cells with normal antigen expression if recovery is the highest priority This special program is disabled by default To enable A Depl07 select Option User settings and O_progs A Depls7 Depletion in sensitive mode via loading of sample in separate 1 mL stages for removal of cells with low antigen expression if purity is the highest priority This special program is disabled by default To enable A Depls7 select Option User settings and O progs The autoMACS Pro Separator is equipped with reusable autoMACS Columns After each cell separation a thorough washing procedure rinses the columns of the autoMACS Pro Separator Short instructions Please find below a list of the available obligatory and optional wash programs for daily use Qrinse Q Standard short wash program that uses Running Buffer Rinse Q Extensive rinsing program that uses Washing Solution and Running Buffer Clean Q Optional very extensive rinsing program that uses storage solution Washing Solution and Running Buffer
18. ment goes into sleep mode Clicking Run will start the experiment Short instructions 25 26 6 3 Monitoring the cell separation process Use the Status menu display to view the overall instrument status For more details please refer to section 2 3 6 3 1 Sample processing status Sample processing statuses are displayed as color coded graphics Graphic Definition Status Waiting Sample processing has not yet started 2 Sample autolabeling is underway 3 Incubation of cells with labeling reagents 4 Sample is being processed e g sample uptake 5 Rinsing 6 Sample processing is completed 7 Progress has been stopped or cancelled Table 6 1 Sample processing statuses displayed in the Status menu Presented here is a cell separation with CD4 MicroBeads human using the autolabeling feature Short instructions Note For daily usage the instrument should not be switched OFF but placed into sleep mode Note To store the autoMACS Pro Separator for a period longer than two weeks run the Store program 6 3 2 Fluid bottle illuminations The autoMACS Pro Separator provides a fluid bottle illumination that facilitates monitoring of the instrument s status even from across the laboratory Code Status User action Green Ready for separation No action required Blue Instrument operating No action required Yellow Not ready for Run wash program separation Rinse or Qrinse before starting a separation Re
19. o the MACS MiniSampler Figure 5 1 The MACS Reagent Rack 4 snaps into position as illustrated above Short instructions Entry of reagents for autolabeling using the 2D code reader 1 Goto the Reagent menu and highlight the position where the vial will be placed in the reagent rack Four positions are available R1 R2 R3 and R4 E Figure 5 2 The rack position RT was selected for reagent assignment 2 Select Read Reagent to activate the 2D code reader 3 Present a reagent vial in front of the 2D code reader Ensure that the 2D code is facing the blinking code reader light Figure 5 3 The optimal reading distance is 0 5 2 5 cm from the code reader cover tilt the vial as shown above 4 After successfully scanning a reagent vial the next available reagent rack position will be automatically highlighted Short instructions 19 20 5 To view details on a scanned Cell Separation Reagent highlight the respective rack position 25 Figure 5 4 Details on the Cell Separation Reagent assigned to rack position R1 are displayed 6 Insertthe reagent vial into the appropriate rack position Manual entry of reagents for autolabeling This option is only recommended if the reagent cannot be identified by the 2D code reader 1 Gotothe Reagent menu and highlight the position where the vial will be placed in the reagent rack Four positions are available R1 R2 R3 and R4 gt Figure 5 5 The rack
20. only and not for therapeutic or diagnostic use autoMACS Pro Separator Short instructions Manufacturer Miltenyi Biotec GmbH Friedrich Ebert Stra e 68 D 51429 Bergisch Gladbach Germany Technical Support Phone 49 2204 8306 830 Fax 49 2204 85197 macstec miltenyibiotec de www miltenyibiotec com Version 3 Short instructions Content 1 1 1 2 1 3 2 1 2 2 2 3 2 3 1 2 3 2 2 3 3 6 1 6 2 6 3 6 3 1 6 3 2 6 4 6 5 7 1 7 2 73 74 Considerations before you start autoMACS Pro cell separation and wash programs Choose appropriate tube rack Prepare cell samples Setting up and priming the autoMACS Pro Separator Setup of the instrument Priming of the instrument Monitoring the instrument status prior to cell separation Status of fluid bottles Status of columns Status of MACS MiniSampler Preparation of samples Select the appropriate tube rack Labeling Cell separation Cell separation after autolabeling Cell separation after manual labeling Monitoring the cell separation process Sample processing status Fluid bottle illuminations Setting the autoMACS Pro Separator in sleep mode Optional Switch OFF the instrument for long term storage Maintenance Rinsing programs Daily maintenance programs Periodic maintenance Column exchange Decision tree for the optimal separation program Short instructions 14 16 18 22 22 24 26 26 27 27 27 28 28 28 29 29 31 Short
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22. wrong sensor cable is connected Gray connect bottle sensor Table 2 1 Status of fluid bottles displayed in the Status menu The color of the bottle symbols indicates the color code of the sensor cables The filling status of the storage solution cannot be detected since it does not contain electrolytes 2 3 2 Status of columns Confirm that the column status is ready Green no action required Red exchange column Grey nocolumn has been installed Short instructions 2 3 3 Status of MACS MiniSampler Confirm that the MACS MiniSampler is correctly installed Figure 2 6 MACS MiniSampler status graphic Left The MACS MiniSampler was successfully installed Right No MACS MiniSampler was detected The instrument is now ready for use Short instructions 13 3 Preparation of samples It is recommended to use single cell suspension for cell separation devoid of cell aggregates and dead cells Typically 1x10 cells are resuspended in 80 uL of buffer and labeled with 20 uL of MicroBeads leading to a total labeling volume of 100 uL When working with higher cell numbers scale up all reagent volumes and total volumes accordingly When working with fewer than 1x10 cells do NOT scale down the volumes but use the same volumes as indicated In the table below the dilution volumes account for the first step of labeling For manual labeling please refer to the respective Cell Separation Reagent data sheet for ongoing proc
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