PDES-02DX-LA Manual - ALA Scientific Instruments
Contents
1. Electronic Instruments for the Life Sciences OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE PDES 02DX PNEUMATIC DRUG EJECTION SYSTEM standard with microJECT VERSION 1 0 npi 2006 npi electronic GmbH Hauptstrasse 96 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com PDES 02DX User Manual Table of Contents a A dieaqats ta de dceeadavaatanaasncune eases a n ka 3 Zu PIDES 2D XS VSCOM oi cs a eee epee hih alee ee alae aoa ea ditties 4 Dl PW a A O 4 2 2 Description of the Promt Paniel 2 12 cas aa A oR aa ed ele 5 2 3 Deseripuon of the Rear Panel on sce ties caidas i ai 7 2 4 microJECT micro Valve Pipette Holder oonnccninicinnncccnnnaccnonncnononccononcnnnnncconnnccnnn nos 8 2 9 I PELALON soe 8 3 Literature aria id db dio 11 AL echnical Data R aed cade RE een eaten 12 version 1 0 page 2 PDES 02DX User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electron2. ann G and H Hatt 1999 Specificity and Sensitivity of a Human Olfactory Receptor Functionally Expressed in Human Embryonic Kidney 293 Cells and Xenopus Laevis Oocytes J Neurosci 19 17 7426 7433 version 1 0 page 11 PDES 02DX User Manual 4 Technical Data Source gas air nitrogen helium etc no aggressive combustible or explosive gases filtered by a 5 um filter element Pressure range input pressure max 5 bar 1 bar 10 Pa 14 5 psi pressure control range 0 2 bar 0 29 psi or 0 4 bar 0 58 psi residual air pressure 10 mbar 0 145 psi 100 mbar 1 45 psi Input and output couplings quick connect type Tubes input 6 mm OD output 4mm OD Status EJECT time indicator red green LED red light channel has been turned on valve is closed green light valve is open or valve not connected Mode selection three position toggle switch TIMER CONT EXT Minimum valve switch time lt 1 ms Digital timer 100 us 9 9 s set by five digit tumble wheel switch 0000 0 9999 9 Pulse input standard BNC connector TTL LO 0 2 5 V HI 2 5 12 V input impedance 10 KQ Pulse output PRESSURE MONITOR standard BNC connector sensitivity 1 V bar or 0 1 V psi output impedance 249 Q TIMING MONITOR standard BNC connector TTL LO 0 1 V HI 5 V output impedance 249 Q Dimensions 19 rackmount cabinet 19 483 mm 10 250 mm 3 5 88 mm Power re
3. e e In the EXT position the duration of the application is determined directly by the duration of the TTL pulse version 1 0 page 8 PDES 02DX User Manual e In the TIMER position the duration is preset by the timer control and time base of the PDES 1 Use the START button to test the system The START button can be used to start ejection in TIMER or EXT mode In TIMER mode pushing the START button starts ejection for the time set by the timer control 5 Figure 1 In EXT mode ejection lasts as long as the START button is pushed Very important notice Even if an eject channel is not activated i e the valve is closed there is a pressure at the pipette Depending on the pressure regulator that is used the pressure at the pipette with valve closed is between 10 mbar and 100 mbar If this is unacceptable for your application the PDES system can optionally be equipped with an additional valve for exhausting Please contact npi electronic for details 1 REMOTE CONTROL option The selected operation mode at the controller determines the way the REMOTE CONTROL works e Inthe CONT mode the remote control unit is disabled e In the EXTERN mode the valve is open as long the REMOTE control switch is activated CONT TIMED continuous START temporarily e In the TIMER mode the internal timer is triggered once by activation of the REMOTE control switch CONT TIMED or START The green LED indicates which channel has be
4. en turned on Figure 4 PDES remote control Note Green LED indicates that the valve is not connected properly or continuous operation version 1 0 page 9 PDES 02DX User Manual Manual mode TIMER A EXT A CONT switch on CONT TIMER A EXT CONT switch off CONT pressure at pipette tip 1V bar OUTPUT TIMING MONITOR EXTERNAL mode TTL INPUT TIMER A pressure at EXT CONT pipette tip 1V odar OUTPUT TIMING MONITOR TIMER mode TTL INPUT TIMER A pressure at EXT CONI pipette tip 1V bar OUTPUT TIMING MONITOR 200 ms Figure 5 operation modes of the PDES 2DX 0120040 if the intemal timer is set to 200 ms version 1 0 page 10 PDES 02DX User Manual 3 Literature m Bickmeyer U Heine M Manzke T amp Richter D W 2002 Differential modulation of Ih by 5 HT receptors in mouse CA1 hippocampal neurons Eur J Neurosci 16 209 218 m Guzman J N Hernandez A Galarraga E Tapia D Laville A Vergara R Aceves J amp Bargas J 2003 Dopaminergic modulation of axon collaterals interconnecting spiny neurons of the rat striatum J Neurosci 23 8931 8940 m Hahnel C 1992 Quantitative Microinjection in H Kettenmann and R Grantyn eds Practical Electrophysiological methods Wiley Liss New York m Hanganu I L amp Luhmann H J 2004 Functional Nicotinic Acetylcholine Receptors on Subplate Neurons in Neonatal Rat Somatosensory Cortex Journal of Neurop
5. erational status red light channel has been turned on valve is closed green light valve is open or valve not connected 5 TIME ms tumble wheel switch Five digit tumble wheel switch to set the ejection time XXXX X ms in TIMER mode 6 START button Push button to start operation The START button can be used to start ejection in TIMER or EXT mode In TIMER mode pressing the START button opens the valve for the time set by 5 In EXT mode pressing the START button opens the valve as long as the button is pressed 7 TTL INPUT connector BNC connector for external operation In TIMER mode a TTL HI 5V signal opens the valve for the time set by 5 In EXT mode a TTL HI 5 V signal opens the valve as long as the signal level is HI 8 TIMING MONITOR connector BNC connector providing a TTL signal that indicates the status of the regulated injection valve HI valve open LO valve closed 9 TIMER A EXT CONT switch Switch to set the operation mode see also chapter 2 4 10 BAR PSI switch Switch to select the unit of the reading of the display 3 and the output at PRESSURE MONITOR 11 11 PRESSURE MONITOR connector BNC connector monitoring the pressure output sensitivity 1 V bar or 0 1 V psi respectively 12 REMOTE CONTROL connector Connector for the REMOTE CONTROL unit see Figure 4 version 1 0 page 6 PDES 02DX User Manual 2 3 Description of the Rear Panel O o o Ea Fig
6. hysiology 92 189 198 m Heck N Kilb W Reiprich P Kubota H Furukawa T Fukuda A amp Luhmann H J 2006 GABA A Receptors Regulate Neocortical Neuronal Migration In Vitro and In Vivo Cereb Cortex doi 10 1093 cercor bhj135 QO Hofmeier G amp Lux H D 1981 The time course of intracellular free calcium and related electrical effects after injection of CaCl into neurons of the snail Helix pomatia Pfl gers Arch 391 242 251 QO Lalley P M 1999 Microiontophoresis and Pressure Ejection in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin Heidelberg New York m Misgeld U 1992 Droplet application of Agonists in H Kettenmann and R Grantyn eds Practical Electrophysiological methods Wiley Liss New York QO Partridge L D Zeilhofer H U amp Swandulla D 1998 Combined Whole Cell and Single Channel Current Measurement with Quantitative Ca Injection or Fura 2 Measurement of Ca In P M Conn Ed Ion Channels Part B Methods in Enzymology 293 Academic Press San Diego m Ponimaskin E Dumuis A Gaven F Barthet G Heine M Glebov K Richter D W amp Oppermann M 2005 Palmitoylation of the 5 Hydroxytryptamine4a Receptor Regulates Receptor Phosphorylation Desensitization and beta Arrestin Mediated Endocytosis Molecular Pharmacology 67 1434 1443 A Wetzel C H Oles M Wellerdieck C Kuczkowiak M Gisselm
7. ic D 71732 Tamm Germany 1 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed 2 AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type 3 STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet 4 TEMPERATURE DRIFT WARM UP TIME All analog electronic systems a
8. onally the signal for pressure control is available via TTL OUT BNC connector Optionally each channel can equipped with an EXHAUST coupling whereby a low retain pressure can be applied analogous to the retaining current used in iontophoretic systems Mode of operation and timing is selected by controls at the front panel If the internal timers are used the eject time is preset by a control and the time base can be selected by a toggle switch Each channel can be turned off by using a switch that is combined with the timing control The operational status is indicated by a red green LED Red means that the channel has been turned on and the valve is closed green indicates that the valve is open and injection takes place The remote control unit is housed in a small box For each channel it has a red LED that indicates if the respective channel is turned on and a toggle switch with a temporary and a fix position for starting drug application The pressure connectors quick connect type are located at the rear panel of the instrument Each channel is equipped with an INPUT coupling where the pressure source is connected an OUTPUT coupling where the injection pipette is connected and an EXHAUST coupling where a low hold pressure can be applied in order to avoid capillary effects at the tip of the injection pipette or to apply a retain negative pressure analogous to the retaining current used in iontophoretic systems The retain pre
9. quirements 115 230 V AC 20 40 W Fuse 0 2A 230V 0 4 A 115V Remote control option Toggle switch and status LED for each channel size 2 5x4x10 cm version 1 0 page 12
10. re sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition i e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient 5 HANDLING Please protect the device from moisture heat radiation and corrosive chemicals HIGH PRESSURE PRECAUTIONS While working with PDES systems and components always adhere to the appropriate safety measures for handling high pressure pneumatic systems Before using the PDES or any external pneumatic device please read the manual and instructions carefully e Always turn off pressure source before connecting or disconnecting the PDES system e Any component used tubes fittings etc must be specified for the maximum possible pressure e Do not use any aggressive combustible or explosive gases Only gases such as air nitrogen carbon dioxide helium etc are allowed e Use only dry oil free filtered gases max 5 um filter is allowed e Maximum input pressure must be below 5 bar 1 bar 100 000 Pa npi recommends an input pressure of 1 bar more than the maximum output pressure version 1 0 page 3 PDES 02DX User Manual 2 PDES 2DX System 2 1 System Description Pressure ejection is a convenient method for applying both ionic and non ionic solutions from micropipettes This method is popular for two reasons First it can apply to almos
11. ro Valve Pipette Holder The two valves which are controlled by the PDES 02D are located in the micro valve pipette holders called microJECT see Figure 3 Tubes and cables of both microJECTs are connected at the rear panel of the PDES system refer to chapter 2 3 Figure 3 microJECT 1 pipette connector 2 connector for EJECT pressure 3 connector for EXHAUST pressure optional 2 5 Operation 1 Connect the gas source to the SOURCE IN connector at the rear panel The gas source must be clean and filtered by a 5 um filter element Important Do not connect sources of OXYGEN or COMBUSTIBLE or EXPLOSIVE gases 1 Connect the microJECT holders to the OUT connectors and VALVE connectors at the rear panel 1 Connect your ejection pipettes to the microJECTs 1 Figure 3 I Turn on pressure and adjust the EJECTION pressure to the desired value using the pressure regulator 1 Connect a digital timing unit to the TTL INPUT BNC connector at the front panel 1 For remote operation connect the remote control unit option to the REMOTE CONTROL connector 1 Turn channel on TIMER control 1 The LED is red when no ejection occurs and green when the pressure is applied to the pipette LI Select the operation mode using the respective toggle switch at the front panel see Figure 1 e In the CONT position the application can be controlled manually i e as long as the CONT position is set pressure is applied to the pipett
12. ssure must be generated by an additional pneumatic device Please contact npi electronic for details The injection pressure is preset by a precision pressure regulator The pressure is displayed on a digital manometer Additional pneumatic devices tubes fittings connectors filters etc are available Please contact npi electronic for details A calibration procedure allowing quantitative drug application is described by Hofmeier and Lux 1981 see chapter 3 version 1 0 page 4 PDES 02DX User Manual 2 2 Description of the Front Panel ee Z U PDES 02DX npi CHANNEL A e Figure 1 PDES 2DX front panel view the numbers are related to those in the text below version 1 0 page 5 PDES 02DX User Manual In the following description of the front panel elements each element has a number that is related to that in Figure 1 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given Since the front panel elements are identical for each channel with identical functions and labels and therefore are numbered and described only once for channel A 1 POWER push button Push button to turn POWER on or off 2 Pressure regulator Rotary knob for regulating the pressure 3 Manometer Digital display for the pressure X XX bar or XXX X psi 4 ON EJECT LED Indicates the op
13. t all solutions Second the amount of solution ejected can be monitored by viewing the tip of the micropipette under a microscope The PDES 2DX is designed for pressure ejection of drugs in physiological and pharmacological studies PDES systems are equipped with high pressure outputs Pmax 2 bar 29 psi or 4 bar 58 psi for drug application with fine tipped micropipettes but can also be operated in low range pressure for controlled droplet application from large tipped pipettes Digital timers and a remote control unit facilitate the use of these instruments In order to speed up drug ejection PDES 2DX system are available with the microJect option These systems are equipped with a micro valve located in an injection holder The micro valve has a switch time of less than 1 ms and a very small volume that has to be controlled resulting in application times in the ms range The pressure is restricted to 2 bar 29 psi or 4 bar 58 psi The PDES 2DX is housed in a 19 cabinet that can be mounted in a rack It comprises of two channels Two systems can be combined to form a 4 channel system Each channel consists of a precision pressure regulator a digital pressure display electronically controlled valves control electronics and a regulated power supply Ejection of drugs can be activated manually or via a TTL input The operational status is indicated by a red green LED The pressure output is monitored at a BNC output 1V bar and additi
14. ure 2 PDES 2DX rear panel view the numbers are related to those in the text below The following connectors are located at the rear panel The connectors are mentioned only for CHANNEL A 1 OUT connector Connector for connecting the micro valve pipette holder microJECT The EJECT pressure is supplied to this connector 2 EXHAUST connector Not installed 3 SOURCE IN connector Connector for connecting the filtered dry and oil free gas source 5 bar max Note npi recommends an input pressure of 1 bar more than the maximum output pressure 4 FUSE Unplug the instrument when replacing the fuse or changing line voltage Turn knob to open Replace fuse only with an appropriate specified type see chapter 4 5 Voltage SELECTOR Rotary switch for selecting the operating voltage 115 V 230 V Caution Always switch to the appropriate voltage before connecting the PDES to power 6 GROUND This connector is linked to the internal system ground which has no connection to the 19 cabinet CHASSIS and the mains ground to avoid ground loops 7 CHASSIS This connector is linked to mains ground green yellow wire protective earth 8 VALVE connector Connector for the electrical connection of the VALVE located in a remote valve box or in the microJECT Important This connector is not used for systems without a remote valve or microJECT version 1 0 page 7 PDES 02DX User Manual 2 4 microJECT mic
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