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Media Wand Media Handling Unit

1. 206 207 212 Fig 4 3 tapflo pump 50 Media Wand User Manual 28 9205 27 Edition AC Reference information 4 301 a 302 Fig 4 4 Media Wand Fig 4 5 TC gaskets and clamps Media Wand User Manual 28 9205 27 Edition AC 51 4 Reference information 4 5 Spare parts 4 5 2 Spare parts list Pos in Item Description Code no Bir Part list ee ng la lz alg E s s J Qa a gt fe E P fe 3 O Zz a 2 1 Pneumatic diaphragm valve Gem 101 11 Actuator DN25 44 5516 05 1 5 Yes No 102 1 2 Diaphragm 600 25 M13 EPDM 44 5506 36 1 5 Yes Yes 2 Pneumatic membrane pump tapflo 2 1 Pump W amp T Kit T103 28 9194 94 i 1 Yes Yes 202 2 2 Pump diaphragm PTFE 2 2 Yes Yes 203 2 3 Diaphragm shaft SS 1 1 Yes o 204 2 4 Valve ball PTFE 4j 4 Yes Yes 2 5 Muffler Not used I a No o 205 206 2 6 Air valve complete 1 1 Yes o 207 2 7 Circlip 2 2 Yes o 208 2 8 Centre block seal PE 2 2 Yes o 209 29 O ring EPDM ae 4 Yes Yes 210 2 10 O ring EPDM 4 4 Yes Yes 211 2 11 O ring EPDM 2 2 Yes Yes 212 2 12 O ring EPDM 2 2 Yes Yes 213 2 13 O ring EPDM 4 4 Yes No 3 Spray tip 301 302 3 1 Spray tip amp tool 28 9219 66 1 1 Yes Yes 302 3 2 Tool spray tip 28 9219 62 1 0 Yes No 4 Sil
2. 21 Start the pump 22 Whenthe slurry level in the container is less than 5 cm 2 from the bottom tilt the container and place the Media Wand in the bottom most location Remove as much as possible 23 Stop the pump Note To be performed if several transport container have been used To minimize loss of media container and system rinse the last container once more Media Wand User Manual 28 9205 27 Edition AC 41 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 6 After run procedures 3 6 After run procedures 3 6 1 Rinsing the system with water 1 Place the Buffer in C hose in clean water Place the Transfer SIB hose to waste Buffer in ronster to Waste Slurry tank 2 Set the INLET valve to C Buffer in and set the OUTLET valve to SIB Transfer Run the pump at 40 l min until the outlet water is clear Stop the pump 3 Place the Media Wand in a waste container set the INLET valve to C Buffer in set the OUTLET valve to SIT Transfer Run the pump at 40 l min until the outlet water is clear 4 Place the Media Wand in a container with clean water Set the INLET valve to B Wand suction set the OUTLET valve to SIT Wand spray Run the pump at 40 l min Stop the pump 5 Discard the water and repeat the procedure with clean water until the rinse water stays clear 3 6 2 Cleaning of Media Wand See Section 2 7 2 3 6 3 After use handling of Chromaflow Packing
3. Fluorine and halenogenated compounds Media Wand User Manual 28 9205 27 Edition AC 9 1 Introduction 1 4 Specifications 10 e Chlorinated solvents such as methylene chloride e Esters e Aromatic hydrocarbons such as toluene e High concentrations of strong acids Table 1 1 Chemical resistance of materials Substance Concentration by Resistance volume 60 90 days Acetic acid 1 7 M OK Ethanol 40 OK Ethylene glycol 50 OK Formaldehyde 1 7M OK Formic acid 10 OK Glycerol 100 OK Hydrochloric acid 0 1M See note 1 Isopropyl alcohol 30 OK Nitric acid 0 1 M OK n Propanol 100 OK Phosphoric acid 25 See note 2 Sodium chloride 0 5 M See note 1 Sodium hydroxide 2M OK Trifluoroacetic acid 0 1 OK Triton X 100 100 OK Tween Tri n butyl 1 0 3 OK phosphate Urea 8M OK Note 1 Not recommended The stainless steel will be affected Note 2 Limited resistance Media Wand User Manual 28 9205 27 Edition AC Introduction 1 1 5 Principle of operation A buffer is sprayed through the Media Wand spray nozzle to initiate the mixing of the sedimented media directly in the transport container Recirculation of the slurry dissolves the sedimented media obtaining a highly concentrated slurry suitable for column packing Fig 1 3 Principle of Media Wand Media Wand User Manual 28 9205 27 Edition AC 11 1 Introduction 1 6 Construction 1 6 Construction 1 6 1 Media Wand a f lt Spray inle
4. UNPACKING PUMP Air pressures to 0 2 Set the NOZZLE CONTROL to 0 3 Ensure the FINE ADJUSTMENT is set to off 4 Connect air supply to the Chromaflow Packing station liquid sprays and or movement of the Media Wand WARNING Incorrect settings before connection to air supply may result in Media Wand User Manual 28 9205 27 Edition AC Operation of Media Wand with Chromaflow Packing station Pack 100 3 3 2 Priming of Chromaflow Packing station and Media Wand Before using the Media Wand and Chromaflow Packing station all inlets and outlets have to be primed 1 Filla container with packing buffer and place the Media Wand and all hoses in it 2 Set the valve controls on the control panel to the positions as shown below INLET valve to B and OUTLET valve to SIB 3 Start the pump by setting the pump pressure to 3 bar Note Start the pump with the FINE ADJUSTMENT regulator unlock and adjust pump pressure to 3 bar N PACKING PUMP UNPACKING PUMP o h Q S uur smg FERES GE Healthcare Chromaflow E Packing station oS 4 Prime for approximately 30 seconds Media Wand User Manual 28 9205 27 Edition AC 33 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 3 Checking the flow of the Chromaflow Packing station 5 Change the valve controls on the control panel to the positions as shown below 6 Prime for 30 seconds 7 St
5. Unit cleaning of Media Wand CONSTFUCTION 0 eee contamination risks control panel ou E emergency stop EX environments F low recommended l intended use L abelling M Media Handling Unit checking the flow cleaning construction labelling preparing priming specifications _ storage solution Media Wand User Manual 28 9205 27 Edition AC 55 Media Wand assembling cleaning 28 construction 12 preparing priming principle of operation SPECICATION i sa ccuaid nie ntakca a a E P preparing a slurry Chromaflow Packing Station eceeeeessssssssssssssssssseeesssssssssssssssssseececcecseeseeseseessusutuntansssnssssssssssssesseeeees 37 edia Handling Unit preparing the Chromaflow Packing station preparing the Media Handling Unit priming of Chromaflow Packing station priming of Media Handling Unit priming of Media Wand principle of operation R FECOMIMENGED T OW AAE E R adhe dobe A E coer eet caters 7 removing transport solution Chromaflow Packing Station sisestus E 35 Media Handling Unit rinsing the system rinsing the system Chromaflow Packing station S shutting down spare parts specification edia Handling Unit edia Wand 100 edia Wand 50 specifications storage solution T transferring slurry Chromaflow Packing station e
6. Wand User Manual 28 9205 27 Edition AC Operation 2 Start the pump at a flow of 40 I min and rinse the interior of the container with a small volume of buffer Note Do not adda volume of buffer leading to a media concentration below 50 Stop the pump Change the valve controls on the control panel to the positions as shown below BUFFER o WAND TRANSFER 0 IN SUCTION TO WAND SPRAY 7 W INLET OUTLET SLURRY G WASTE TANK OUTLET NK TRANSFER CONTROL Start the pump When the slurry level in the container is less than 5 cm 2 from the bottom tilt the container and place the Media Wand in the bottom most location Remove as much as possible Stop the pump To be performed if several transport container have been used To minimize loss of media container and system rinse the last container once more 27 2 Operation 2 7 After run procedures 28 2 7 After run procedures Immediately after the media transfer procedure the system must be rinsed CAUTION Do not leave the system with media 2 7 1 Rinsing the system with water 1 Place the Buffer in hose in clean water Place the Transfer and Waste hoses to waste Set the INLET valve to BUFFER IN set the OUTLET valve to TRANSFER TO and set the TRANSFER CONTROL valve to SLURRY TANK Run the pump at 40 l min until the outlet water is clear Set the TRANSFER CONTROL valve to WASTE OUTLET and run
7. height of media in the container 2 Attach a decanting device to the Media Wand optional 3 Remove the cap on the media container Note Ensure the media is well sedimented 4 Set the valve controls on the control panel to the positions as shown below BUFFER 9 WAND TRANSFER a WAND IN SUCTION TO SPRAY N LA N P p Sf N INLET OUTLET SLURRY a WASTE TANK OUTLET TRANSFER CONTROL 5 Set the TRANSFER CONTROL switch to WASTE OUTLET 6 Place the Waste outlet hose in a container 7 Position the Media Wand above the liquid surface 8 Start the pump with a minimum pump pressure Adjust to approximately 2 bar Ensure pump is running 9 Insert the Media Wand just below the liquid surface into the container CAUTION Do not allow the Media Wand to touch the media surface as this may affect media integrity 22 Media Wand User Manual 28 9205 27 Edition AC Operation 2 10 Lower the Media Wand slowly and carefully to a position just below the liquid surface It is not a problem if the Media Wand occasionally is above the liquid surface Remove as much of transport solution as possible 11 Lift the Media Wand while pump is running 12 Turn off the pump 13 Ifa Decanting device has been used replace with a spray tip 2 6 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank The preparation of the slurry starts with the addition of buffer to obtain an appropriate medi
8. media 10 Stop the pump 11 Immediately continue with step 12 CAUTION Do not leave the system with media Do not shut down the system without rinsing the pump and flow paths as remaining media will sediment and block the system Media Wand User Manual 28 9205 27 Edition AC 39 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 5 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank 12 Set the valve controls on the control panel to the positions as shown below 13 Start the pump with a flow of 40 l min 14 When the slurry level in the container is less than 5 cm 2 from the bottom tilt the container and place the Media Wand in the bottom most location Remove as much as possible CAUTION Do not scratch the bottom or walls of the container 15 Stop the pump 16 Keep the Media Wand in the container 17 Setthe valve controls on the control panel to the positions as shown below Buffer in 40 Media Wand User Manual 28 9205 27 Edition AC Operation of Media Wand with Chromaflow Packing station Pack 100 3 18 Start the pump at a flow of 40 l min and rinse the interior of the container with a small volume of buffer Note Do not adda volume of buffer leading to a media concentration below 50 19 Stop the pump 20 Change the valve controls on the control panel to the positions as shown below
9. pressure to the pump PUMP PRESSURE INDICATOR Indicates the pump pressure e PUMP CONTROL The switch is set to ON or OFF INLET valve Sets the inlet flow to BUFFER IN or WAND SUCTION OUTLET valve Sets the outlet flow to TRANSFER TO or WAND SPRAY TRANSFER CONTROL When OUTLET valve is set to TRANSFER TO this valve sets the flow to SLURRY TANK or WASTE Media Wand User Manual 28 9205 27 Edition AC 15 1 Introduction 1 6 Construction 16 Media Wand User Manual 28 9205 27 Edition AC Operation 2 2 Operation WARNING Wear safety glasses safety gloves and protective clothing 2 1 Preparing the Media Wand 2 1 1 Assembling the Media Wand Carefully introduce the inner pipe into the outer pipe Ensure there is a gasket in the Tri Clamp connection 1 Place aTri Clamp over the connection between the pipes and tighten firmly by hand 2 If Slurry preparation will be performed Assemble the spray tip finger tight j to the lower part of the Media Wand Optional if removal of transport solution will be performed attach a decanting device to the lower part of the Media Wand See Section 4 6 1 Accessories 2 1 2 Connecting hoses to Media Wand 1 Connect hoses to the Suction and Spray ports of the Media Wand 2 Use gaskets and fasten with Tri Clamps 2 2 Preparing the Media Handling Unit WARNING NO SERVICABLE PARTS INSIDE Do
10. station See Chromaflow Packing stations Instructions for Use 42 Media Wand User Manual 28 9205 27 Edition AC Reference information 4 4 Reference information 4 1 Specifications 4 1 1 Media Wand The Media Wand is available in two lengths e Media Wand 100 1 m length for large 60 L media containers e Media Wand 50 0 5 m length for smaller 5 to 10 L media containers Table 4 1 Technical specifications of Media Wand Part Material Outer and inner pipe Stainless steel 316L Spray nozzle tip PEEK 4 1 2 Media Handling Unit The tapflo pump TU 103 may run dry without any problem The pump can be stopped either by setting the pump switch to OFF or by setting the valve controls to 0 The pressure from the system will stop the pump automatically This will not do any damage to the pump Note The pump restarts immediately when the valves are re opened Table 4 2 Technical specifications of Media Handling Unit Subject Specification Noise lt 70 dB A System volume 1082 ml Hose volumes 195 ml m ID 21 mm Material Frame Stainless steel 316 Material Tubes Stainless steel 316L Media Wand User Manual 28 9205 27 Edition AC 43 4 Reference information 4 2 General specification for Media Wand 50 4 2 General specification for Media Wand 50 4 2 1 Technical data Specification Design pressure Design temperature Piping ID Stainless steel surface finish interior parts Stain
11. temperature TS 2 C 30 C Supply air pressure 5 7 barg Air consumption 500 Ni min Pump capacity 100 l min GE Healthcare GE Healthcare Bio Sciences AB Bj rkgatan 30 l 751 84 Uppsala Sweden O Fig 1 1 The Media Handling Unit name plate 8 Media Wand User Manual 28 9205 27 Edition AC Introduction 1 1 4 Specifications 1 4 1 Air supply Dry clean oil free compressed air with a minimum pressure of 6 bar max 8 bar Capacity curve of the built in tapflo TU103 pump is shown below Air consumption 7 4 10 6 17 7 247 0 3 0 7 90 100 I min 25 US g min 20 UK g min Fig 1 2 Capacity curve of the tapflo TU103 pump Example External pressure air pressure is 4 bar To obtain a pump flow of 40 l min the pump pressure is set to 3 bar which will result in an air consumption of 0 3 m min 1 4 2 Chemical resistance of Media Wand and Media Handling Unit Table 1 1 is a guide to the resistance of Media Wand and Media Handling Unit to chemicals and solvents commonly used in process chromatography The information has been compiled from published material It should be noted that the effects of a chemical will be more severe at higher temperatures and pressures and that combined effects have not been taken into consideration In general the use of the following chemicals should be avoided e Powerful oxidizers such as peroxides e
12. CIP manifold wn 28 Cleaning of Media Handling Unit without CIP manifold 29 Contamination TiS RS sos niae E EE Filling Media Handling Unit with storage solution als Shutting down the Media Handling Unit sssssssssssssssssssseessssssesseee Media Wand User Manual 28 9205 27 Edition AC Vv 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 1 Preparing the Chromaflow Packing Station ou 31 3 2 Priming of Chromaflow Packing station and Media Wand 33 3 3 Checking the flow of the Chromaflow Packing station 34 3 4 Removing transport solution from a media container 35 3 5 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank scsssssssseeessssccccssssssssssseessessee 37 3 5 1 PRO COOH CS sc225 65 sass cssseebsivadebasckavcatotcsiva vba ha beastssbvobeiavtaeOatasstesnctndabedie treats 37 3 6 After run procedures o esssssssessssssssscssssssssssssssssessssccccsssssssssusssssssseeeeeessessess 42 3 6 1 Rinsing the system with water we 42 3 6 2 Cleaning Of Media Wand cesesssssssssssssssssssssssssssssussssssscssssessssesseesessssessssssese 42 3 6 3 After use handling of Chromaflow Packing station ccecscssssssssssee 42 4 Reference information WD SPECS HOPS sinensis iia 43 4 1 1 Media Wand we 43 4 1 2 Media Handling UN iit cccccssessssssscscsesessessssssssssseeeees we 43 4 2 General specifica
13. GE Healthcare Media Wand Media Handling Unit User Manual Important user information CE Certifying All users must read this entire manual to fully understand the safe use of Media Wand and Media Handling Unit WARNING that must be followed to avoid personal injury Do not proceed until all stated conditions are clearly understood and met The WARNING sign highlights instructions CAUTION The CAUTION sign highlights instructions that must be followed to avoid damage to the product or other equipment Do not proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product This product meets all requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used inthe same state as it was delivered from GE Healthcare except for alterations described in this manual Contents 1 Introduction 11 1 2 13 14 1 4 1 1 4 2 15 1 6 1 6 1 1 6 2 1 6 3 intended Usuaris i n A a ada 7 Ainm OP the ProdUCt nesnenin i A E RS i 7 Labelling of the Media
14. Handling Unit oe cccscssssssssssesseeesscsssssees 8 Specifications a Air SUDD U saisan E E REREN Chemical resistance of Media Wand and Media Handling Unit ccccccsssscssssssscsscssessessssssssssssssssusssssssssseesesseeseseeseeesesssssssssssea 9 Principleof operati soaisss cccsccstsovassizsscassseccescestrtsssazessssosanccoscszeseesvanuavis 11 C NStrUCHO Nissinen ia n a E 12 Medid Wand asuen E E 12 Med dia Handling Unit sesesisseraimesiiniiniieiiannnaa 13 GONE DADEN kaata rnia 15 2 Operation 2 1 2 1 1 2 1 2 2 2 2 3 24 2 5 2 6 2 6 1 2 7 2 7 1 27 2 213 27 4 275 2 7 6 LAA Preparing the Media Wand wvceeesssssssssssssssssssesessssssesssssssssssnsesessseceeesee 17 Assembling the Media Wand E A Connecting hoses to Media Wand 17 Preparing the Media Handling Unit oc ecsscssscsssssssssseeessseeeeesee 17 Priming of Media Handling Unit and Media Wand wees 19 Checking the flow of the Media Handling Unit 0 we 2l Removing transport solution from a media container wl Adjusting media concentration preparing a slurry and transferring slurry to slurry tank oiceceecsssssssssesessssscccsssssssssseeeeees 23 Procedi Ocie aaa E a A 23 After run procedures 28 Rinsing the system With water ssssssssssssssssssssssssscssccsssesseceseceseessssnessssees 28 Cleaning Of Medid WON u eecessssssssssssssssesssssssssssssssssssssssssssssssssecseesessesseseeeeees 28 Cleaning of Media Handling Unit with
15. SPRAY O A X X l Y j R L INLET OUTLET SLURRY 0 WASTE TANK OUTLET N TRANSFER CONTROL CAUTION Do not allow the spray nozzle to come in contact with the sedimented media surface as this may affect media integrity The spray nozzle should be positioned just above it 5 Place the Media Wand in the media container The spray nozzle should be positioned just above the media surface 6 Start the pump with a flow of 40 2 I min Note Set the appropriate pump pressure to obtain the flow of 40 2 l min 7 During the addition of buffer lower the Media Wand slowly towards the bottom of the container during approximately 30 seconds CAUTION Do not exceed the desired volume CAUTION Do not let the Media Wand come in direct contact with the bottom or walls of the container as this may affect media integrity 24 Media Wand User Manual 28 9205 27 Edition AC Operation 2 8 When the required volume of buffer has been added change the valve controls as shown below BUFFER o WAND TRANSFER q WAND IN SUCTION TO SPRAY DADAN D Q Ky a So L YL 4 7 N INLET OUTLET SLURRY 0 WASTE TANK OUTLET TRANSFER CONTROL 9 Continue to move the Media Wand slowly up and down and also along the container walls until all media is in slurry CAUTION To maintain integrity of media never exceed the maximum time of 5 minutes 60 L media or 2 minutes 10 L If media has been stored in cold room add app
16. TION TO SPRAY SZ NVA SZ N f s N On gt S INLET OUTLET SLURRY o WASTE TANK OUTLET Y ra So TRANSFER CONTROL 3 Set the pump pressure to 3 bar and start the pump Prime for approximately 30 seconds 4 Set the INLET switch to BUFFER IN Set the OUTLET switch to WAND SPRAY Set the TRANSFER CONTROL switch to 0 BUFFER o WAND TRANSFER 0 WAND IN SUCTION TO SPRAY WA xX YZ NG KY re R Z an pee Z a INLET OUTLET SLURRY WASTE TANK OUTLET TRANSFER CONTROL Media Wand User Manual 28 9205 27 Edition AC 19 2 Operation 2 3 Priming of Media Handling Unit and Media Wand 5 Prime for 30 seconds 6 Setthe INLET switch to WAND SUCTION Set the OUTLET switch to WAND SPRAY Set the TRANSFER CONTROL switch to 0 Prime for 30 seconds BUFFER H WAND TRANSFER o WAND IN SUCTION TO SPRAY NZ x E V L Y F L r P ae a Y a INLET OUTLET SLURRY o WASTE TANK OUTLET E TRANSFER CONTROL 7 Set the INLET switch to WAND SUCTION Set the OUTLET switch to TRANSFER TO Set the TRANSFER CONTROL switch to SLURRY TANK Prime for 30 seconds BUFFER 7 WAND TRANSFER a WAND IN SUCTION TO SPRAY y K XX ara lt A N INLET OUTLET SLURRY Q WASTE TANK OUTLET TRANSFER CONTROL 8 Stop the pump 20 Media Wand User Manual 28 9205 27 Edition AC Operation 2 2 4 Checking the flow of the Media Handling Unit The recommended flow when preparing media slurry is 40 2 l m
17. a concentration As the Media Wand is lowered during the buffer addition the preparation of the slurry is initiated When all buffer has been added the recirculation of media and buffer starts and the slurry preparation continues As additional buffer will be introduced to the media when the transport container and Media Wand are rinsed it is recommended to use a media concentration of approximately 70 when preparing the slurry The media concentration during column packing should not be below 50 For specifications of hose and system volumes see Section 4 1 Note Ensure the system is primed and a spray tip is assembled on the Media Wand Ensure there is a hose connected to a slurry tank Ensure there is buffer connected to the Media Handling Unit Buffer in position CAUTION When preparing and handling media slurry always hold the Media Wand by hand Never let it stand in media containers as this may affect media integrity 2 6 1 Procedure 1 If not performed in Section 2 5 measure the height of the sedimented media 2 Calculate the volume to obtain a media concentration of 70 3 Set amark on the container for the desired total volume Media Wand User Manual 28 9205 27 Edition AC 23 2 Operation 2 6 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank 4 Set the valve controls on the control panel to the positions as shown below BUFFER 0 WAND TRANSFER 0 WAND IN SUCTION TO
18. and User Manual 28 9205 27 Edition AC Introduction 1 Introduction 1 1 Intended use Media Wand is designed to be used for e Removing transport solution from media containers e Adding buffer and preparing a media slurry in a media container e Transferring the media slurry to a slurry tank The Media Wand can be used together with e Media Handling Unit or with e Chromaflow Packing station Pack 100 only The Media Wand should not be used in EX environments or for other purposes than those described in this manual The user of the Media Wand should be trained in handling chromatography media in general It is recommended to first try the Media Wand in a medium that will not be re used The recommended flow when using the Media Wand is 40 2 l min 1 2 Aim ofthe product The aim of the product is to facilitate the handling of media containers The generation of a homogenous media slurry before column packing is a cumbersome process involving heavy lifting and movements especially for larger containers The use of Media Wand will considerably reduce both the handling time and work effort 1 Introduction 1 3 Labelling of the Media Handling Unit 1 3 Labelling of the Media Handling Unit The Media Handling Unit is labelled as follows N Media Handling Unit Code no 28 9227 69 Serial number XXX a Year of manufacturing XXX a Operating
19. ct the air supply to the Media Handling Unit Priming 3 Turnon the pump and adjust the pressure to approximately 3 bar 4 Rinse all lines by re circulating the sodium hydroxide for at least 30 seconds per line Cleaning 5 Shut off the pump and leave the Media Handling Unit for 60 minutes Rinsing 6 Remove the Buffer inlet hose from the sodium hydroxide tank and connect it to a supply of WFI Water for injection 7 Start the pump still with the pump set at 3 bar 8 Rinse all lines with WFI for 30 seconds per line 9 Continue pumping WFI through the pump and measure pH on the outgoing flow When the pH on the outlet side is equivalent to pH on the inlet side shut off the pump 2 7 5 Contamination risks If other types of media will be used with the Media Wand and or Media Handling Unit ensure the equipment is properly cleaned to avoid contamination 2 7 6 Filling Media Handling Unit with storage solution If the system will not be used for some time it should be filled with 20 ethanol 1 Start the pump and fill the system with ethanol 2 Set the OUTLET switch to 0 3 Set the INLET switch to 0 4 Remove the hoses and block the hose connections Media Wand User Manual 28 9205 27 Edition AC 29 2 Operation 2 7 After run procedures 2 7 7 Shutting down the Media Handling Unit 1 2 3 30 Set all valve switches to 0 Set pump pressure to minimum and the pump switch to OFF Remove the supp
20. dia Handling Unit Media Wand User Manual 28 9205 27 Edition AC For contact information for your local office please visit www gelifesciences com contact www gelifesciences com GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Chromaflow and Media Wand are trademarks of GE Healthcare companies All third party trademarks are the property of their respective owners 2007 General Electric Company All rights reserved First published April 2007 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9205 27 AC 11 2007
21. encer 401 4 1 Silencer and PEEK net 28 9219 64 1 1 No No 5 TC gaskets amp clamps 303 5 1 TC gasket 50 35mm EPDM 44 0515 01 5 1 Yes Yes 501 5 2 TC gasket 50 22mm EPDM 44 7133 01 1 2 Yes Yes 502 55 TC Clamp TC50 5 SS 44 7134 00 i 3 Yes No 52 Included in W amp T Kit T103 Media Wand User Manual 28 9205 27 Edition AC Reference information 4 4 6 Ordering information Code no Item 28 9227 64 Media Wand 50 28 9227 67 Media Wand 100 28 9227 69 Media Handling Unit 4 6 1 Accessories Code No Item 44 7134 00 Tri Clamp 28 9227 70 Decanting Device 50 28 9227 71 Decanting Device 100 28 9227 73 ClP manifold Media Handling Unit 28 9230 74 PTFE tubing L 2m TC50 ID 22 Media Wand User Manual 28 9205 27 Edition AC 53 4 Reference information 4 6 Ordering information 54 Media Wand User Manual 28 9205 27 Edition AC Index A CICCOSSOM OS arnesa a a a a an 53 adjusting media concentration Chromaflow Packing station Media Handling Unit on after run procedures Chromaflow Packing station after run procedures Media Handling Unit aim air supply assembling the Media Wand Cc checking the flow of the Chromaflow Packing station o sccescccsscsssssssessssssssssssesssceseeseseeesseeeeseestteee 34 checking the flow of the Media Handling Unit Chemical resistance esses Chromaflow Packing station checking the flow preparing CIP of Media Handling Unit cleaning of Media Handling
22. et the valve controls on the control panel to the positions as shown below Buffer in CAUTION Do not allow the spray nozzle to come in contact with the sedimented media surface as this may affect media integrity The spray nozzle should be positioned just above it 5 Place the Media Wand in the media container The spray nozzle should be positioned just above the media surface 6 Start the pump with a flow of 40 2 l min Note Set the appropriate pump pressure to obtain the flow of 40 2 l min 7 During the addition of buffer lower the Media Wand slowly towards the bottom of the container during approximately 30 seconds CAUTION Do not exceed the desired volume CAUTION Do not let the Media Wand come in direct contact with the bottom or walls of the container as this may affect media integrity 38 Media Wand User Manual 28 9205 27 Edition AC Operation of Media Wand with Chromaflow Packing station Pack 100 3 8 When the required volume of buffer has been added change the valve controls as shown below 9 Continue to move the Media Wand slowly up and down and also along the container walls until all media is in slurry CAUTION To maintain integrity of media never exceed the maximum time of 5 minutes 60 L media or 2 minutes 10 L If media has been stored in cold room add approximately one minute to the mixing time This will compensate for the increased viscosity of the colder
23. in A pump pressure of approximately 3 bar will usually give this flow Note Do not exceed a flow rate of 40 l min Ensure the system is primed before checking the flow 1 Record the weight of an empty container 2 Place the hose from Media Handling Unit connection position 4 Buffer in in a container with water 3 Place the Media Wand in the water tank 4 Ensure Emergency stop is reset 5 Set the valve controls on the control panel to the positions as shown below BUFFER g WAND TRANSFER 0 WAND IN SUCTION TO SPRAY D X y V YA X y PX VN Ge SZ gt AN WS y gt Sf INLET OUTLET SLURRY 0 WASTE TANK OUTLET a TRANSFER CONTROL 6 Start the pump with a pump pressure of 3 Bar 7 Recirculate the liquid until the flow is stable 8 Turn off the pump 9 Place the Media Wand in the empty container and start the pump with a pump pressure of 3 bar Fill the container during a set time 10 Weigh the container and calculate the obtained flow 11 Repeat the procedure with an adjusted pump pressure until a flow of 40 2 min has been obtained Media Wand User Manual 28 9205 27 Edition AC 21 2 Operation 2 5 Removing transport solution from a media container 2 5 Removing transport solution from a media con tainer Media is delivered in a transport solution usually 20 ethanol Note To avoid loss of media it is recommended that the transport solution is transferred to a separate container 1 Measure the sedimented
24. in a appropriate media concentration As the Media Wand is lowered during the buffer addition the preparation of the slurry is initiated When all buffer has been added the recirculation of media and buffer starts and the slurry preparation continues As additional buffer will be introduced to the media when the transport container and Media Wand are rinsed it is recommended to use a media concentration of approximately 70 when preparing the slurry The media concentration during column packing should not be below 50 For specifications of hose and system volumes see Section 1 4 Note Ensure the system is primed and a spray tip is assembled on the Media Wand Ensure there is a hose connected from position SIB to a slurry tank Ensure there is buffer connected to the Chromaflow Packing station position C Buffer in position CAUTION When preparing and handling media slurry always hold the Media Wand by hand Never let it stand in media containers as this may affect media integrity 3 5 1 Procedure 1 If not performed in Section 3 4 measure the height of the sedimented media 2 Calculate the volume to obtain a media concentration of 70 3 Set amark on the container for the desired total volume Media Wand User Manual 28 9205 27 Edition AC 37 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 5 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank 4 S
25. less steel surface finish exterior parts 5 bar g 2 C 30 C 22 1mm Ra lt 0 6 um Electropolished Ra lt 0 6 um Electropolished 1 Interior and exterior parts are wetted Wand dimensions Chemical resistance Height 500 mm Weight 6 kg 4 2 2 Materials of construction Structural material Tube 316L Wetted amp pressure retaining material Piping 316L Seals and gaskets EPDM Spray nozzle PEEK Refer to User Manual for Media Handling Unit Section 1 4 2 4 2 3 Documentation Material certificates and welding documentations are delivered on the Media Wand Documentation CD placed in the Media Wand package 44 Media Wand User Manual 28 9205 27 Edition AC Reference information 4 4 2 4 Applicable codes and standards Mechanical design GE Healthcare standard 4 2 5 Battery limits Mechanical Description Dimension ID mm Flange type Inlet Outlet 22 1 Tri Clamp 50 4 3 General specification for Media Wand 100 4 3 1 Technical data Specification Design pressure 5 bar g Design temperature 2 30 C Piping ID 22 1mm Stainless steel surface finish interior parts Ra lt 0 6 um Electropolished Stainless steel surface finish exterior parts Ra lt 0 6 um Electropolished 1 Interior and exterior parts are wetted Wand dimensions Height 1000 mm Weight 7kg Media Wand User Manual 28 9205 27 Edition AC 45 4 Reference information 4 3 Gene
26. ly of compressed air Media Wand User Manual 28 9205 27 Edition AC 2 Operation of Media Wand with Chromaflow Packing station Pack 100 3 Operation of Media Wand with Chromaflow Packing station Pack 100 IMPORTANT For detailed information of the handling of the Chromaflow Packing station refer to Chromaflow Packing stations Instructions for Use For preparation of the Media Wand see Section 2 1 3 1 Preparing the Chromaflow Packing station Connect hoses to the Chromaflow Packing station according to the table below Use gaskets and fasten with Tri Clamps firmly by hand Ward suction a Sti Transfer to Waste LE J T ra tank en UNPACKING PUMP i psal t om mm 4 Z Position Connection B Wand suction C Buffer in SIT Wand spray SIB Transfer to Waste Slurry tank 31 Media Wand User Manual 28 9205 27 Edition AC 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 1 Preparing the Chromaflow Packing station 32 To facilitate the handling of Media Wand a 90 connection at position B and SIT can be used The hose from the SIB outlet is used for both transfer to waste and slurry tank To have different hoses connect a T valve at that position gt Q NOZZLE CONTROL na d TOP NOZZLE PACKING PUMP UNPACKING PUMP A Q S n on ecient nnsa GE Healthcare Chromaflow oe Packing station 1 Set the PACKING PUMP and
27. not open the Control unit box Service and planned maintenance should be performed by personnel authorized by GE Healthcare only WARNING If the risk of static electricity exists use hoses with conductivity characteristics Recommended length is 2 m Media Wand User Manual 28 9205 27 Edition AC 17 2 Operation 2 2 Preparing the Media Handling Unit 1 Connect hoses to the Media Handling Unit according to the table below Use gaskets and fasten with Tri Clamps firmly by hand Position Connection 1 Transfer to waste 2 Wand spray 3 Wand suction 4 Transfer to slurry tank 5 Buffer in 2 Setall three valve controls to 0 3 Set the pump switch to OFF and the pump pressure to minimum WARNING Incorrect settings before connection to air supply may result in liquid sprays and or movement of the Media Wand 4 Connect the air supply to the air supply port For specification of compressed air see Section 1 4 1 18 Media Wand User Manual 28 9205 27 Edition AC Operation 2 2 3 Priming of Media Handling Unit and Media Wand Before using the Media Wand and Media Handling Unit all inlets and outlets have to be primed 1 Filla container with packing buffer and place the Media Wand and all hoses in it 2 Set the INLET switch to WAND SUCTION Set the OUTLET switch to TRANSFER TO Set the TRANSFER CONTROL switch to WASTE OUTLET BUFFER 9 WAND TRANSFER 9 WAND IN SUC
28. onsumption estimated Type 5 7 barg 500 Ni min 5 7 bar g Instrument air Media Wand User Manual 28 9205 27 Edition AC 47 4 Reference information 4 4 General specification for Media Handling Unit 48 4 4 3 Materials of construction Structural material Stand 316L Castors with locking device 316 Wetted amp pressure retaining material Piping 316L Pump interior PTFE HDPE Valve EPDM Pressure gauge 316L 316Ti Seals and gaskets EPDM Chemical resistance Refer to User Manual for Media Handling Unit Section 1 4 2 4 4 4 Documentation Material certificates and welding documentations are delivered on the Media Wand Documentation CD placed in the Media Wand package 4 4 5 Applicable codes and standards Mechanical design GE Healthcare standard CE directive compliance Machinery Directive 98 37 EC Media Wand User Manual 28 9205 27 Edition AC 4 4 6 Battery limits Reference information 4 Mechanical Description Dimension ID mm Flange type Inlet Outlet 22 1 Tri Clamp 50 Air Supply Quick connect 4 5 Spare parts 4 5 1 Spare parts schematic Fig 4 1 Media Handling Unit Media Wand User Manual 28 9205 27 Edition AC 49 4 Reference information 4 5 Spare parts 101 102 a Lly l Fig 4 2 Diaphragm valve 209 210 202 211 204
29. op the pump 3 3 Checking the flow of the Chromaflow Packing sta tion The recommended flow when preparing media slurry is 40 2 l min A pump pressure of approximately 3 bar will usually give this flow Note Do not exceed a flow rate of 40 l min Ensure the system is primed before checking the flow 1 Record the weight of an empty container 2 Place connect the hose from Chromaflow Packing station connection position C Buffer in in to a container with water 3 Place the Media Wand in the water tank 34 Media Wand User Manual 28 9205 27 Edition AC Operation of Media Wand with Chromaflow Packing station Pack 100 3 4 Set the valve controls on the control panel to the positions as shown below Buffer in 5 Start the pump with a pump pressure of 3 Bar 6 Recirculate the liquid until the flow is stable 7 Turn off the pump 8 Place the Media Wand in the empty container and start the pump with a pump pressure of 3 bar Fill the container during a set time 9 Weigh the container and calculate the obtained flow Repeat the procedure with an adjusted pump pressure until a flow of 40 2 I min has been obtained 3 4 Removing transport solution from a media con tainer Media is delivered in a transport solution usually 20 ethanol Note To avoid loss of media it is recommended that the transport solution is transferred to a separate container 1 Measure the sedimented height of media in the container 2 At
30. ral specification for Media Wand 100 46 4 3 2 Materials of construction Structural material Tube 316L Wetted amp pressure retaining material Piping 316L Seals and gaskets EPDM Spray nozzle PEEK Chemical resistance Refer to User Manual for Media Handling Unit Section 1 4 2 4 3 3 Documentation Material certificates and welding documentations are delivered on the Media Wand Documentation CD placed in the Media Wand package 4 3 4 Applicable codes and standards Mechanical design GE Healthcare standard Media Wand User Manual 28 9205 27 Edition AC 4 3 5 Battery limits Reference information 4 Mechanical Description Dimension ID mm Flange type Inlet Outlet 22 1 Tri Clamp 50 4 4 General specification for Media Handling Unit 4 4 1 Technical data Specification Pump valve control unit System pump Pump flow rate max Design pressure Design temperature Valve Pressure gauge Piping ID Stainless steel surface finish wetted parts Stainless steel surface finish exterior Pneumatic Semi automatic Pneumatic diaphragm pump 100 l min 5 barg 2 C 30 C Ball valve Pneumatic actuator 0 10 bar g 22 1mm Ra lt 0 6 um Electropolished Ra lt 3 2 um Electropolished Media Handling Unit dimensions Footprint Height Weight 850 x 630 mm 895 mm 70 kg 4 4 2 Media Handling Unit requirements Pneumatical requirement Air Supply Air c
31. roximately one minute to the mixing time This will compensate for the increased viscosity of the colder media 10 Stop the pump 11 Immediately continue with step 12 CAUTION Do not leave the system with media Do not shut down the system without rinsing the pump and flow paths as remaining media will sediment and block the system Media Wand User Manual 28 9205 27 Edition AC 25 2 Operation 2 6 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank 12 Setthe valve controls on the control panel to the positions as shown below BUFFER o WAND TRANSFER o WAND IN SUCTION TO SPRAY MZ X p V JN A lt eo Qa A lt S Z INLET OUTLET SLURRY 0 WASTE TANK OUTLET A OC YON TRANSFER CONTROL 13 Start the pump with a flow of 40 l min 14 When the slurry level in the container is less than 5 cm 2 from the bottom tilt the container and place the Media Wand in the bottom most location Remove as much as possible CAUTION Do not scratch the bottom or walls of the container 15 Stop the pump 16 Keep the Media Wand in the container 17 Set the valve controls on the control panel to the positions as shown below BUFFER H WAND TRANSFER Fi WAND IN SUCTION TO SPRAY VER V Yr xX yy N NS N Ne f PA INLET OUTLET SLURRY g WASTE TANK OUTLET TRANSFER CONTROL 26 Media Wand User Manual 28 9205 27 Edition AC 18 19 20 21 22 23 Note Media
32. t Fig 1 4 The 0 5 m Media Wand 50 Suction outlet Outer pipe nl lt A Suction outlet lt A Spray nozzle a Spray inlet 4 Rubber gasket jaa Inner pipe Fig 1 5 The 0 5 m Media Wand 50 disassembled 12 Media Wand User Manual 28 9205 27 Edition AC Introduction 1 Suction inlet lt q Spray nozzle tip PEEK Fig 1 6 The nozzle tip area of the Media Wand 1 6 2 Media Handling Unit Media Wand 100 Control panel Media Wand 50 Emergency Stop Air supply port Pump Valves Hose connections Muffler Fig 1 7 Schematic layout of the Media Handling Unit with attached Media Wands Media Wand User Manual 28 9205 27 Edition AC 13 1 Introduction 1 6 Construction Transfer to waste Wand spray Transfer to slurry tank Emergency Stop button Fig 1 9 Emergency stop and Reset buttons on the Media Handling Unit e To stop the Media Handling Unit press the Emergency Stop button e To reset the Emergency stop push the Emergency Stop button and turn it counterclockwise Press the Reset button 14 Media Wand User Manual 28 9205 27 Edition AC Introduction 1 1 6 3 Control panel MEDIA HANDLING UNIT PUMP CONTROL H amp S A N A TRANSFER PUMP PRESSURE CONTROL INDICATOR Fig 1 10 The layout of the control panel on the Media Handling Unit PUMP PRESSURE REGULATOR Regulates the air
33. tach a decanting device to the Media Wand optional 3 Remove the cap on the media container Note Ensure the media is well sedimented Media Wand User Manual 28 9205 27 Edition AC 35 3 Operation of Media Wand with Chromaflow Packing station Pack 100 3 4 Removing transport solution from a media container 4 Set the valve controls on the control panel to the positions as shown below 5 Place connect the Transfer to Waste Slurry tank hose in to a container 6 Position the Media Wand above the liquid surface 7 Start the pump with a minimum pump pressure Adjust to 2 bar Ensure pump is running 8 Insert the Media Wand just below the liquid surface into the container CAUTION Do not allow the Media Wand to touch the media surface as this may affect media integrity 9 Lower the Media Wand slowly and carefully to a position just below the liquid surface It is not a problem if the Media Wand occasionally is above the liquid surface Remove as much of transport solution as possible 10 Lift the Media Wand while pump is running 11 Turn off the pump 12 If a decanting device has been used replace with a spray tip 36 Media Wand User Manual 28 9205 27 Edition AC Operation of Media Wand with Chromaflow Packing station Pack 100 3 3 5 Adjusting media concentration preparing a slurry and transferring slurry to slurry tank The preparation of the slurry starts with the addition of buffer to obta
34. the pump at 40 min until the outlet water is clear Stop the pump Place the Media Wand in a waste container set the INLET valve to BUFFER IN set the OUTLET valve to WAND SPRAY Run the pump at 40 l min until the outlet water is clear Stop the pump Place the Media Wand in a container with clean water Set the INLET valve to WAND SUCTION set the OUTLET valve to WAND SPRAY Run the pump at 40 I min Stop the pump Discard the water and repeat the procedure with clean water until the rinse water stays clear 2 7 2 Cleaning of Media Wand Dismount the Media Wand clean the parts and or clean the wand in an autoclave WARNING HAZARDOUS CHEMICALS Do not CIP the Media Wand using pumped NaOH through the wand due to the risk of NaOH spray 2 7 3 Cleaning of Media Handling Unit with CIP manifold Perform a CIP procedure using an assembled CIP manifold optional on the Media Handling Unit WARNING CORROSIVE CHEMICALS Some chemicals for example NaOH are corrosive and therefore dangerous to health Avoid spillage and wear protective glasses If a CIP manifold is not used perform a CIP procedure according to Section 2 7 4 Media Wand User Manual 28 9205 27 Edition AC Operation 2 2 7 4 Cleaning of Media Handling Unit without CIP manifold Equipment setup 1 Connect hoses to all outlets and place the non connected ends in a 100 L tank with at least 20 liters of 1 M NaOH 2 Conne
35. tion for Media Wand 50 veseeccsssssssssssssssseeeessessee 44 4 2 1 Technicaldatt Sersan A E RANAR 44 4 2 2 Materials of construction 44 4 2 3 Documentation 0 eccescsssesessesessseees wa 44 4 2 4 Applicable codes and standards m45 4 2 5 Battery liit Siepnisimainaaionne annn p ai 45 43 General specification for Media Wand 100 wees 45 4 3 1 Techical COE erosscssecsisssscrssiesssnasbsssnsessizssnetesssocensssts 45 4 3 2 Materials of construction wu 46 4 3 3 Documentation esseescsseesessseseeseeees wae 46 4 3 4 Applicable codes and StANCOrdS ceessssssssssssssssssssssssssssssecsessesessesseeeeees 46 4 3 5 Battery TNA NES 2c cccssincosnanctsssvesesnsiassitznczaashccysstenesssiecenndtncesteipestastoss dittesienceisse 47 4 4 General specification for Media Handling Unit 47 4 4 1 Technical COLO se seciaiisieresstecicteleastid wu 47 4 4 2 Media Handling Unit requirements wa 47 4 4 3 Materials OF COMSEUCTION sicssatssccctescascsssssssosbssesbenossssebennadsssostbiasepebseadbebebaes 48 44 4 Docume EGE OP aeaa aR EA RIOS ANEKO NE 48 4 4 5 Applicable codes and standards 48 4 4 6 Battery liMit csssssssssssssssssseeee 49 4 5 Spare Parts oe 49 4 5 1 SPAre PArtS SCHEMATIC cccesssssssssssssssssssssssssssssssssssscsessessesseseesessessesssssnsasssass 49 4 5 2 Spare pants MSE scrscstat zir e E A ANENA 4 6 Ordering information aa 4 6 1 ACCESSO E Seenaa AAA A EEOAE vi Media Wand User Manual 28 9205 27 Edition AC 1 Media W

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