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133577 Rev01_IVIS Spectrum CT Hardware

1. 2 In the Living Image software save any important data then exit the program 3 Turn off the main power at the rear of the instrument 4 Using the 5 32 hex key remove the eight screws that hold the filter access panel in place on the upper right side of the instrument Figure 10 2 The panel is held in place by four pins until it is pulled forward CAUTION Be careful not to damage the light sealing gasket when removing the panel D NOTE The panel has a machined recess on the inner left hand side towards the front of the instrument Noting this orientation is helpful for reinstalling the panel IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance Figure 10 2 Filter wheel access panel Machined recess a D NOTE Starting with step 5 wear lint free powderless gloves to prevent smudging the filters 5 Notice that the 1 2 shaft on the right side of the filter wheel compartment has a carriage clamp lock with a handle Turn the handle counter clockwise to loosen the clamp so that it can slide freely over the shaft 6 Grip the filter wheel by the metal rim and pull the filter wheel carriage forward until it stops Be careful not to touch the glass filters or to dislodge the two drive belts Since the carriage slides out about eight inches only two or three of the filters will be visible The filter wheel holds the filters numbered 1 11 7 To remove a
2. Department of Human Services 800 NE Oregon Street Suite 640 Portland OR 97232 2162 971 673 0499 http public health oregon gov Pages Home aspx PENNSYLVANIA Bureau of Radiation Protection Rachel Carson State Office Bldg P O Box 8469 Harrisburg PA 17105 8469 717 787 2480 http www dep state pa us brp default htm PUERTO RICO Radiological Health Division Department of Health P O Box 70184 San Juan PR 00936 8184 787 274 7802 http www salud gov pr RHODE ISLAND Office of Facilities Regulation Division of Environmental and Health Services 3 Capitol Hill Room 206 Providence RI 02908 5097 401 222 4520 http www health ri gov hsr facilities radiological index php SOUTH CAROLINA Bureau of Radiological Health Dept of Health Environment Control 2600 Bull Street Columbia SC 29201 803 545 4420 http www scdhec gov health radhlth x ray htm SOUTH DAKOTA Office of Health Care Facilities Licensure and Certification 615 East 4th St Pierre SD 57501 1700 605 773 3356 http doh sd gov Licensure Default aspx IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 76 Table 11 2 US Radiation Authorities continued TENNESSEE Division of Radiological Health 37911 Middlebrook Pike Knoxville TN 37921 Telephone 865 594 5577 http www state tn us environment rad TEXAS Bureau of Radiation Control State D
3. Monthly Safety Checks The following safety checks should be performed every month 1 All daily safety checks 2 All weekly safety checks 3 Activate the Emergency Stop switch to verify operation All indication of X ray generation should cease when the switch is pushed in Y NOTE This will cut all power X rays will need to be generated to perform this test 4 Reset the Emergency Stop switch by turning the red knob clockwise The knob should pop out 5 Initialize the system 6 Re arm the X ray source and restart X ray generation from the Living Image software Annual Safety Checks The following safety checks should be performed every 12 months 1 All safety checks performed on a daily weekly and monthly basis 2 A full radiation survey performed by a qualified person 10 3 Cleaning the IVIS Spectrum CT If necessary wipe the exterior surface of the IVIS Spectrum CT instrument with a soft cloth If the system requires more aggressive cleaning or sterilization contact Caliper technical support DANGER DO NOT use sprays or fluids to clean the exterior or interior of the module Do not allow fluids of any kind to penetrate the electronics cabinet under any circumstances Sprays and liquids that come into contact with the IVIS Spectrum CT may result in damage to the system or electrocution WARNING Do not use fluids or moistened towels to clean any part of the instrument where electrical or
4. The imaging chamber is a highly specialized device consisting of the imaging chamber housing moveable platform with a rotating CT imaging stage a lens system with f stop control and synchronized filter wheels that control the spectral content of the luminescent or fluorescent images Figure 7 4 Figure 7 4 Imaging Chamber An LED based illumination system provides the means to acquire photographic information before imaging The imaging chamber is equipped with gas anesthesia inlet and outlet ports for use with the optional Caliper XGI 8 Gas Anesthesia System The imaging chamber is light tight so that no light penetrates from the outside after the door is locked The solenoid latch ensures that the door cannot be inadvertently opened during an imaging session The interior of the imaging chamber is constructed from non phosphorescent and non fluorescent materials to prevent internal light contamination that could compromise sample measurements The chamber and door are made from steel of sufficient thickness to prevent radiation leakage WARNING Under no circumstances should you attempt to make any mechanical modifications to the imaging chamber the door or the interlock switches 7 7 Optical Components Imaging System Lenses The IVIS Spectrum CT is equipped with a rotating lens carousel that provides demagnification of 1 5X 2 5X 5X and 8 8X power Figure 7 6 The filter wheel settings are selected in the
5. Caliper technical support An X ray safety survey must be performed when the instrument is installed or if abnormal mechanical shocks occurred during movement A survey is also to be performed when the IVIS Spectrum CT has undergone any form of service in which the safety interlocks have been adjusted or any of the shielding has been removed and re installed After servicing if the safety interlocks are not operating properly or if the X ray shielding is not properly re installed serious injury can result when operating the system Conducting an X ray safety survey is the only way to confirm proper shielding and interlock operation WARNING When performing a radiation survey of the IVIS Spectrum CT please comply with your own laboratory radiation regulations or contact Caliper technical support for further assistance Owners and operators of the IVIS Spectrum CT are responsible for complying with all regulations in the country where the equipment is operated This includes all local state and federal regulations In some states of the US it may be necessary to register radiation sources with the governing state and or local public health agencies before operating the instrument Equipment registration may be required immediately or within 30 days of acquiring the equipment Owners and operators of the IVIS Spectrum CT are responsible for contacting the appropriate public health agencies for registration information that pertains
6. the circuit breaker that is incorporated into the rear panel power entry module main power switch should not trip Frequent tripping of this circuit breaker may indicate an underlying electrical problem If this occurs unplug the system and contact Caliper technical support If the circuit breaker is occasionally tripped perform the following steps 1 Confirm that the rear panel switch is off and the power cord is plugged into the console and the wall socket 2 Turn on the rear panel main power switch 3 Turn on the computer and if necessary start the Living Image software 4 Initialize the system click Initialize in the control panel within the Living Image software The status light is red and turns green when the system is initialized Wait until the camera has cooled to the demand temperature before acquiring images Computer and Camera Connections Never disconnect or wiggle the rear panel computer or camera connections when the IVIS Spectrum CT is powered on If you suspect that any of the rear panel computer connections are loose follow the steps below Close the Living Image software and shut down the computer Turn off the X ray key switch Turn off the rear panel circuit breaker switch A oW Mes Check the connections between the computer and the rear panel of the IVIS Spectrum CT Tighten any loose connection 5 Turn on the rear panel main power switch The status light is red 6 Turn on the computer a
7. 10 cm from the surface of the equipment under maximum operating conditions Caliper certifies that the IVIS Spectrum CT has achieved the objectives of the a ICRP 60 recommendations of annual public dose limit of 100mrem ICRP 103 recommendations of annual public dose limit of 100mrem US OSHA workplace annual public dose limits of 100mrem and other international public safety standards and regulations IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards It is unlikely that properly trained individual using the IVIS Spectrum CT will receive an annual dose that exceeds these public dose limit levels 5 4 IVIS Spectrum CT Safety Systems The IVIS Spectrum CT has many different safety features that are intended to keep the operator safe from radiation exposure Some of these features are discussed in this manual as well as the Safe Operating and Emergency Procedures for the operation of the Spectrum CT Cabinet X ray System part no 133919 CAUTION To prevent the development of unsafe operating conditions in the instrument do not tamper with the instrument and follow the maintenance procedures in Chapter 10 on page 57 Radiation Shielding The IVIS Spectrum CT is considered a cabinet X ray system because all of the radiation is confined to the inside of the metal structure This is accomplished by using steel of a sufficient thickness to block X rays of the energies produced by the X ray source
8. 11 1 Canadian Provincial Territorial Federal Radiation Protection Contacts Non medical X rays continued Ontario Radiation Protection Service Occupational Health and Safety Ontario Ministry of Labour 81A Resources Road Weston ON M9P 3T1 Tel 416 235 5765 Fax 416 235 5926 Manitoba Head of Radiation Protection Medical Physics Division Cancer Care Manitoba 675 McDermot Ave Winnipeg MB R3E 0V9 Tel 204 787 2213 Fax 204 775 1684 Saskatchewan Radiation Safety Unit Department of Labour 400 1870 Albert St Regina SK S4P 4W1 Tel 306 787 4538 Fax 306 787 2208 Alberta Radiation Health amp Safety Specialist Alberta Human Resources 8 Employment 10808 99th Ave 8th Floor Edmonton AB T5K 0G5 Tel 780 415 0612 Fax 780 422 0014 British Columbia Radiation Protection Services BC Centre for Disease Control 655 12th Avenue West Vancouver BC V5Z 4R4 Tel 604 660 6630 Fax 604 660 6628 Yukon Workers Compensation Health amp Safety 401 Strickland St Whitehorse YT Y1A 5N8 Tel 867 667 5376 Fax 867 393 6279 IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 80 Table 11 1 Canadian Provincial Territorial Federal Radiation Protection Contacts Non medical X rays continued Northwest Territories Workers Safety amp Compensation Commission Northwest Territories and Nunavut PO Box 8888 Yellowknife NT X1A 2R
9. 52 Accessing the Area Under the Imaging Platform elles 53 Imaging Chamber Emergency Access 000 0c eee eee 54 Initial Steps os sie ogee EHE EIE A Dr ra Med ok 54 Mechanically Deactivating the Solenoid 0000 02 eeeeaee 54 Care and Maintenance celle ern 57 Surveying the IVIS Spectrum CT for Radiation Leakage 57 Conducting the X Ray Radiation Survey llle 57 Maintenance and Safety Checks llle 57 Daily Safety CHeCkS scx oie a eee uc E RIS Oh e suse a La ta caw a 57 Weekly Safety Checks uasa ass ai aaa ad a ae ae eee eee eee 58 Monthly Safety Checks 0 0 0 58 Annual Safety Checks sm ea Bde a wake dove Ee ea eed es Rea ee ee Re E 58 Cleaning the IVIS Spectrum CT 00 ee 58 Approved Cleaning Solutions ocooccccooccc ee 59 Cleaning the Imaging Platform llle 59 Cleaning the Rotating Stage uaaa 59 Cleaning the Lens Protection Window 00 cece eee eee ees 60 Contents iv Changing the Emission Filters for Fluorescence Imaging 60 Servicing the Chiller Unit o ooooccoooooooo 62 Required tems sued ume xe Ha da eae daa ee E RON ara es Bo Re E 62 CoolantReplenishment Procedure 0 00 cece eee ee ee ees 62 Moving the System issued Re Bis air ads ea eas 65 Removing the Dome 000 cee ee 65 Moving the System 2 0
10. X ray source must be immediately turned off Under normal circumstances it should be left in the ON position ul IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 7 4 Key Selector Switch and Lost Keys X ray safety regulations require controlled access to the IVIS Spectrum CT The objective of this requirement is to prevent untrained and unauthorized personnel from operating the X ray functionality of the instrument The key operated switch on the IVIS Spectrum CT fulfills this requirement when used in conjunction with the user s own written radiation safety procedures The switch is designed so that the key can only be removed in the OFF position When an authorized user is finished using the instrument the key should be removed from the switch Two keys are provided with the instrument A master key person usually manages the keys It isa good practice to store the spare key in a safe location If the keys are lost contact Caliper technical support 7 5 Thermoelectric Chiller The thermoelectric unit is a thermoelectric water chiller Figure 7 3 Water from the thermoelectrically cooled CCD camera is pumped to the thermoelectric chiller where the water is cooled and returned to the camera Figure 7 3 Thermoelectric Chiller Coolant amp Return pc Power Coolant supply IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 32 7 6 Imaging Chamber
11. a soft damp cloth only Do not allow fluids of any kind to enter the system interior under any circumstances For more details on cleaning the system see page 58 3 2 X ray Safety A A Y WARNING This equipment produces X rays when energized A A Y WARNING The IVIS Spectrum CT should be operated only by personnel who have been trained in radiation safety and the operation and safety instructions contained in this manual Caliper also recommends that personnel who operate the equipment or are close proximity to the equipment use a radiation film badge or other type of appropriate personal dosimeter The IVIS Spectrum CT has multiple safety interlocks that prevent X ray generation when the door is open Figure 3 1 The primary interlock switch prevents any generation of X rays unless the door is completely closed A secondary redundant door interlock prevents X ray generation when the instrument door is opened See Safety Interlocks on page 21 for more details on the primary and secondary safety interlocks IVIS Spectrum CT Hardware Manual Chapter 3 Warnings 11 Figure 3 1 IVIS Spectrum CT safety interlocks Secondary redundant door interlock Primary interlock ON Off switch solenoid activated lock 3 3 Mechanical Safety The IVIS Spectrum CT has many internal motorized components that can only move when the door is closed and locked The imaging platform moves frequently during routine use
12. does not receive radiation prior to imaging Image acquisition begins when the X ray tube is at full power and a series of images is collected Figure 7 2 X ray source and detector Bearings Rotating Table Shutter X Ray Flat Panel Translllumination X Ray Source Stage 7 3 X ray System Control Panel The front panel located to the right of the imaging chamber door has two switches and two indicator lights that are associated with the instrument X ray function The main ON OFF switch that controls the electrical power to the instrument is on the rear of the IVIS Spectrum CT Activating this switch provides power to the instrument but does not permit the X ray source to be energized unless the following conditions are met The imaging chamber door is completely closed and locked by the solenoid lock All interlocks are closed and panels mounted The Emergency Stop switch is in the ON out position See the note below The key selector switch is turned ON Som es Ss The amber switch has been pushed and the light is ON indicating that all safety interlocks are functioning properly The X ray source cannot be energized from the Living Image software until these conditions have been fulfilled NOTE The Emergency Stop switch is not intended as a main X ray source control and should not be used to turn the X ray function ON or OFF on a routine basis It should only be used in the unlikely situation where the
13. ensuring that the safety and maintenance procedures specified in this manual are performed Frequently verify that all safety procedures are followed the IVIS Spectrum CT has not been modified and no safety interlocks have been disabled Follow all guidance supplied by your local state or provincial radiation protection authority If that guidance conflicts with the Caliper supplied information either written or spoken contact Caliper technical support so that the conflicts can be resolved Keep records of X ray surveys instrument repairs or other data required by the radiation protection authority or your institution Keep registration certificates compliance or safety audit reports instrument inspections training records and the list of authorized users Keep records of any accident or investigation reports or worker complaints IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards 24 p Create a Training Plan If your radiation protection authority or your institution requires a radiation training plan you may find the following suggestions helpful a Use the documentation supplied with IVIS Spectrum CT for specific training instructions for the instrument Use supplemental information for more in depth coverage of topics such as radiation safety Identify radiation hazards associated with the use of the IVIS Spectrum CT Characteristics of radiation Units of dose a Discuss the various war
14. fiber optic cables make connections Do not use fluids of any kind near the filter wheel assembly Turn off the electrical power to the IVIS Spectrum CT by turning off the rear panel switch before engaging in cleaning operations that use fluids The imaging chamber power switch is located on the rear of the instrument IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance Approved Cleaning Solutions The compounds shown in Table 10 11 do not damage the internal finish of the IVIS Spectrum CT imaging chamber and are suitable as cleaners if required Do not use any solution not included in this list In particular avoid strong bases bleach or acids that may potentially damage the unit and compromise its operation E IMPORTANT Do not spray cleaning solutions inside the imaging chamber Table 10 1 Approved cleaning solutions for the imaging chamber Cleaning Solution Manufacturer Cidexplus Solution 3 4 glutaraldehyde Johnson amp Johnson Medical Sporicidin Sterilizing Solution 1 56 phenol Sporicidin International Clidox s Disinfectant Pharmacal Research Laboratories Inc 70 methyl alcohol 30 deionized water solution 70 ethyl alcohol 30 deionized water solution NOTE Caliper makes no claims as to the sterility of the imaging chamber after cleaning with the solutions in Table 10 1 Please refer to the manufacturer s literature for information as to the applicabilit
15. filter from the filter wheel a Slowly rotate the filter wheel until the filter of interest is exposed b Use a 2 Phillips head screw driver to loosen the flat head screw ONE TURN The screw has a captured O ring that retains the glass emission filter c Use two gloved fingers to remove the filter Alternatively gently push the filter up with a gloved finger 8 To clean a filter use Dust Off compressed gas to apply an air stream at an angle to the filter surface otherwise dust particles could be driven into the filter coating CAUTION Do not blow air onto a filter in the filter wheel as dust could be spread to other optical components 9 To install a filter insert the filter into the filter wheel recess and tighten the O ring screw until it bottoms out about one turn y NOTE Make sure that the filter retaining screw and O ring are fully seated to prevent interference or rubbing against components above them The top of the filter metal ring should be nearly flush with the wheel The filter encapsulating metal ring has one side that is thinner than the other The thin part of the ring should face downward in the wheel recess so that the arrow that is printed on the side of the filter points up 10 Push the filter wheel carriage back into the instrument by placing one finger on the rim of the upper filter wheel IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance 62 11 While holding the carriage
16. images The 3D anatomical location and strength of luminescent sources are computed from CT and luminescent image data 1 2 Caliper Technical Support For technical support please contact Caliper at Telephone 1 877 522 2447 US 1 508 435 9500 E mail tech support caliperls com Fax 1 508 435 3439 Mail Caliper Life Sciences US Corporate Headquarters 68 Elm Street Hopkinton MA 01748 3 2 Important Safety Instructions Definitions Instrument Labels on page 5 Instructions on page 5 X Ray Safety and Radiation Hazards Regulations on page 6 Fluorescence Light Source Safety on page 7 Environmental Consideration for System Components on page 7 Power Considerations on page 8 Cleaning or Moving the System Components on page 9 Other Equipment or Chemicals on page 9 Servicing on page 9 2 1 Definitions When you see a caution warning or voltage symbol pay particular attention to the safety information presented CAUTION A caution note indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury and or mechanical damage It is also used to alert against unsafe practices It reminds you that all safety instructions should be read and understood before installation operation maintenance or repair of this instrument WARNING A warning alerts you to an action or condition that can potentially cause serious personal injury or loss of life Mechanical damage may also resul
17. keep loose objects away from the edges of the platform where they could become jammed CAUTION DO NOT defeat any of the instrument safety interlocks Do not place anything under the imaging platform The imaging platform cannot move unless the door is closed and locked If the platform moves when the door is unlocked shut down the system and contact Caliper technical support The IVIS Spectrum CT contains several delicate optical and mechanical components Do not touch these components and avoid using sprays or other contaminates that may damage lenses or other optical mechanical components 3 4 Chemical and Biological Safety Normal operation may involve the use of samples that are pathogenic toxic or radioactive Itis your responsibility to ensure that all necessary safety precautions are taken before such materials are used Dispose of all waste materials according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the IVIS Spectrum CT before requesting service by Caliper technical support Ask your laboratory safety officer to advise you about the level of containment required for your application and the proper decontamination or sterilization procedures Handle all infectious samples according to good laboratory procedures and methods to prevent the spread of disease IVIS Spectrum CT Hardware Manual Chapter 3 Warnings 12 3 5 Eye Safety and Burn Hazard The IVIS Spe
18. on the operating requirements see Electrical Power Requirements on page 25 VOLTAGE The IVIS Spectrum CT can operate at multiple voltages 100 120 220 240 VAC however you are not permitted to change the input voltage to any of the system components The instrument should only be powered by the set voltage which is marked on the outside of the product itself Several internal modifications are required for voltage change If the operating voltage must be changed contact Caliper technical support see page 3 Power Cord Protection Power supply cords should be routed so that they are unlikely to be walked on or pinched by items placed upon or against them Pay close attention to receptacles and to the points of connection between cords and equipment If it becomes necessary to replace the power cord the replacement power cord set should be adequately rated for the voltage and current and suitably selected and certified as required in the country of end installation A separate surge protector is provided for the computer and other accessory equipment Facilities should be adequately wired according to local building codes IVIS Spectrum CT Hardware Manual Chapter 2 Important Safety Instructions Power Outages If the IVIS Spectrum CT experiences a loss of supply power turn off the power switch for all components and do not restart the system until reliable power has been restored For more details on how to restart the s
19. otherwise its electrical connector may become disconnected If this happens contact Caliper technical support Figure 10 4 Remove the lower rear panel using a hex head wrench to expose the chiller unit The chiller unit sits on a tray that must be pulled out to access the unit 3 To access the chiller a Move any cables or tubes out of the way so that the tray with the chiller can be pulled out freely IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance b Pull out the tray with the chiller Figure 10 4 Y NOTE The chiller unit has its own power switch located at the rear of the unit The power switch is not visible until the unit is pulled out Make sure that the power switch is not accidentally turned off when you slide the tray out or back into the console Figure 10 5 Pull out the chiller unit tray c Remove the chiller unit from the tray and place it on the floor 4 Pull up the chiller fill cap and check the coolant level Figure 10 6 The coolant should be visible 5 Add coolant if necessary CAUTION DO NTO OVERFILL the chiller Over filling the tank can cause the fluid to run down the chiller walls and contact the power plug a Using the fill bottle add small quantities of the 25 ethanol 75 distilled water solution to the tank until the coolant level reaches the bottom of the fill port Be careful not to spill the liquid b W
20. ray generator in OFF position Primary X ray indicator X ray armed switch and indicator button Emergency OFF switch X ray ON Indicators The IVIS Spectrum CT is equipped with three indicators that show the status of X ray generation Table 5 2 IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards p Table 5 2 X ray ON indicators Indicator Description The X ray indicator light on the main console control panel This indicator is X RAY ON independent of the other two indicators If it malfunctions the remaining two indicators are operational The X ray indicator in the IVIS Acquisition Control Panel in the Living Image software A light under the translucent dome at the top of the instrument which is visible from 360 degrees This figure shows the light with the dome removed This light illuminates the entire dome when X rays are being generated Safety Interlocks WARNING Never defeat the primary and the redundant safety interlocks Defeating the safety interlocks could result in serious injury of death The IVIS Spectrum CT has multiple safety interlocks that prevent X ray generation when the door is open The primary interlock switch is activated as soon as you close the door The primary interlock switch is an ON OFF switch that is combined with a solenoid activated lock It prevents any generation of X rays unles
21. s technical assistance then any damage to the System due to such movement shall not be covered under the initial warranty or any extended warranty and Licensee shall pay Caliper s standard service rates for repair of such damage If a problem develops during the Limited Warranty the Customer shall contact Caliper technical support for assistance THE FOREGOING LIMITED WARRANTY IS THE CUSTOMER S SOLE AND EXCLUSIVE REMEDY ANDIS IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED CALIPER SHALL NOT BE LIABLE FOR SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING BUT NOT LIMITED TO LOSS OF ANTICIPATED BENEFITS OR PROFITS LOSS OF SAVINGS OR REVENUE PUNITIVE DAMAGES LOSS OF USE OF THE SYSTEM OR ANY ASSOCIATED EQUIPMENT COST OF CAPITAL COST OF ANY SUBSTITUTE EQUIPMENT OR FACILITIES DOWNTIME THE CLAIMS OF ANY THIRD PARTIES INCLUDING CUSTOMERS AND INJURY TO PROPERTY RESULTING FROM THE PURCHASE OR USE OF THE SYSTEM OR ARISING FROM BREACH OF THE WARRANTY BREACH OF CONTRACT NEGLIGENCE STRICT TORT OR ANY OTHER LEGAL OR EQUITABLE THEORY EVEN IF CALIPER KNEW OF THE LIKELIHOOD OF SUCH IVIS Spectrum CT Hardware Manual Chapter 4 Legal Notices 14 DAMAGES CALIPER SHALL NOT BE LIABLE FOR DELAY IN RENDERING SERVICE UNDER THE LIMITED WARRANTY OR LOSS OF USE DURING THE PERIOD THAT THE SYSTEM IS BEING REPAIRED vi Some countries states or provinces do not allow the exclusion or limitation of implied warranties or the limitation o
22. session The solenoid lock serves two purposes 1 locks the door during imaging including X ray operation and 2 acts as a primary interlock switch for X ray operation A second redundant interlock prevents X ray generation when the door is opened or ajar This redundant interlock operates by removing part of the X ray circuit when the door is open The copper rod attached to the door is the part that completes the circuit 8 5 Centering Subjects in the Field of View A green illuminated pattern is projected onto the imaging platform when the instrument door is opened The pattern of nested lines represents the field of view FOV A B C and D It provides a helpful guide for centering subjects in the field of view IVIS Spectrum CT Hardware Manual Chapter 8 Operating the IVIS Spectrum CT Figure 8 3 Pattern projected onto the imaging platform o FOV D E FOVC FOV B Ebr E lt FOV A r 8 6 Optical Imaging Basics The IVIS Spectrum CT has both bioluminescent and fluorescent imaging capabilities Optical imaging measures the light emitted by light producing luminescent or fluorescent reporters such as luciferase or fluorescent proteins In the luminescent imaging mode no excitation or emission filters are used Selecting the Auto Exposure option in the control panel is recommended in most cases To change from luminescent to fluorescent operation select fluorescent mode in the IVIS Ac
23. the light is split equally to four diffusers to provide uniform stage illumination When bottom or transillumination is selected the excitation light is directed to one of the holes in the aperture plate located in the stage surface This removable plate has a 9 x 19 array of 3 mm diameter holes which provide discrete illumination locations that are user selectable NOTE Only the Single Mouse manifold can be used with transillumination Caliper provides optional accessories for alternative illumination use with the IVIS Spectrum CT a Blank plate For use with the 5 mouse manifold compatible with epi illuminated fluorescent imaging or luminescent imaging The blank plate fits into a recess on the rotating turntable Mouse Imaging Shuttle Provides a means of transferring a single mouse between other imaging instruments and the IVIS Spectrum CT without disrupting the subject position The shuttle also provides a small amount of compression to the mouse in order to enhance the optical signal levels See the Living Image Software Manual for more details on how to use the epi and transillumination systems Selecting Emission and Excitation Filters The IVIS Spectrum CT includes a factory installed filter set The filters are mounted on a rotating filter wheels For details on how to select an emission filter see the Living Image Software Manual for the IVIS Spectrum CT part no 134222 Emission filters can be remo
24. the platform is not accessible unless the normal operation mode is overridden To access the space below the imaging platform 1 Remove all items from the imaging stage that are taller than 2 cm and close the door 2 In the IVIS Acquisition Control panel click Service button The platform moves to the service position and the door can be opened 3 Place the stage lock down bar in position Figure 9 3 Be careful not to disturb the platform heater cable or the gas tubing located in this area To return the platform to the operating position 1 Return the lock down safety bar to the stowed position under the front edge of the imaging platform and close the door 2 In the IVIS Acquisition Control panel click the Load button The imaging platform returns to the normal operating position IVIS Spectrum CT Hardware Manual Chapter 9 Troubleshooting Figure 9 3 Stage lock down bar The stage lock down bar is located Pull the bar down from the at the bottom front edge of the stowed position and place it as imaging platform stowed position shown above Return the bar to its stow position before returning the imaging platform to the operating position 9 8 Imaging Chamber Emergency Access There is a remote possibility that the solenoid door lock could fail in the locked position which would prevent you from opening the imaging chamber door after an imaging session If this occurs the solenoid can be manually de
25. to installation of the IVIS Spectrum CT If you need assistance with this requirement contact Caliper technical support For more details and contact information see Safe Operating and Emergency Procedures for the Operation of the IVIS Spectrum CT Cabinet X ray System This document was provided with the pre installation instructions 6 IVIS Spectrum CT Hardware Manual Chapter 2 Important Safety Instructions A WARNING A Caliper employee will conduct a radiation leakage survey and safety tests when the IVIS Spectrum CT is installed Caliper employees are trained in radiation safety However check with your local radiation control authority to determine the specific radiation survey requirements at your facility If necessary have a qualified expert other than a Caliper employee survey the installation before operating the instrument 2 5 Fluorescence Light Source Safety WARNING The IVIS Spectrum CT is equipped with a strong visible fluorescence light source Do not stare directly into this source 2 6 Environmental Consideration for System Components Location for the IVIS Spectrum CT Before installation consider the proper environment for the IVIS Spectrum CT components Install the equipment in an environment where The temperature does not change or fluctuate widely Choose an environment where the temperature is maintained between 18 24 C 65 75 F The humidity does not exceed 80 No strong electri
26. 0 ccc eee ees 23 Create a Training Plan 0 0 nh 24 Specifications isses ee eee ee a ee nn 25 Electrical Power Requirements 0 000 e eee 25 CCD Camera Optical Imaging 0 0 ee eee 25 Optics 2h ccc aw caste aan cian Oh ee eh ene Peon oo 26 A PD 26 X ray Detector 4 Gace cae he bbw eee See ee tE Pe EN Re SOROR NUR eee ea eee 26 Fluorescent Imaging Components 00 00 cee eet eee 27 Environmental Temperature and Humidity 000000 o 27 Weight and Dimensions s sees sa daama daii ee 27 IVIS Spectrum CT Components 28 CCD Camera for Optical Imaging 00000 eee ee 29 X ray Source Detector and Filters 0 0 0 ee eee 30 X ray System Control Panel 0 0 ee eee eas 30 Key Selector Switch and Lost Keys 0 00 cece eee eee eens 31 Thermoelectric Chiller cee eee eee eee eas 31 Imaging Chamber 32 Optical Components o 32 Imaging System Lenses 0 ocoocococo ee 32 Emission and Excitation Filters 0 00 ee eas 33 Specimen Warming System eee eee nett 34 Rotating Stage and Anesthesia Connections llle 34 Single Mouse Manifold oococcocococo res 35 Chapter 8 Chapter 9 Chapter 10 Contents iii Gas Connections scs scuole ls exc REBEL EE LR Rue 36 Cleaning the Anesthesia Manifolds and Stages llsssus 38 X ray Door and Panel Interloc
27. 000 electron volts The X rays are produced by the collision of high energy electrons with a tungsten metal target in a vacuum tube When high voltage is applied between a heated cathode and the tungsten anode electrons are stripped from the cathode and are accelerated into the anode The collision of those electrons produces X rays There are two atomic processes capable of producing X rays from these collisions One process known as bremsstrahlung from the German braking radiation generates X rays by the rapid deceleration of the high energy electrons as they interact with the repulsive electron field of the tungsten target metal The second X ray generating process results from the high energy free electrons interaction with the atomic orbitals of the target metal The Spectrum CT X ray tube generates X rays using both of these processes The tube generates a spectrum of X rays from approximately 10keV to the maximum of 50keV Two thin filters one made of copper and the other of aluminum can be placed in the X ray beam to reduce unusable low energy radiation A thick filter made from tungsten is placed in front of the beam during source warm up in order limit the animal s X ray exposure IVIS Spectrum CT X ray Source Tube The X ray generating tube is located behind an interlocked panel on the rear of the IVIS Spectrum CT The tube is neither accessible nor serviceable by the user The X ray tube high voltage power supply and other e
28. 0c nhe 66 Chapter 11 Contact and Ordering Information 67 PerkinElmer Contact Information llle 67 Technical Support 4 00 6 rue med rl reb ome aed eo ae 67 C liper Sales d sivas a ees ac a uas et chee ie RR HT a v ie eu 67 Ordering Information sesi eas argitara he 67 US Radiation Authorities llle 69 Canadian Radiation Authorities eee RR RI th 78 1 Welcome About the IVIS Spectrum CT Caliper Technical Support on page 3 This manual explains how to operate and maintain the IVIS Spectrum CT instrument and provides guidelines for obtaining bioluminescent fluorescent or CT images Please read this manual carefully before using the IVIS Spectrum CT to ensure safe optimum performance and a maximum service life from the instrument For instructions on using the system software please see the Living Image Software Manual for the IVIS Spectrum CT part no 134222 If you have questions regarding this manual or the IVIS Spectrum CT please call Caliper technical support see page 3 1 1 About the IVIS Spectrum CT The IVIS Spectrum CT is an in vivo imaging instrument for molecular and anatomical imaging The IVIS Spectrum CT imaging system Figure 1 1 includes a CCD camera mounted on a light tight imaging chamber Camera power supply a Thermoelectric cooling unit Fluorescence light source a X ray tube source and CMOS X ray detector a Windows based comp
29. 3 Tel 867 669 4407 or 1 800 661 0792 Fax 867 873 0262 Federal Health Canada H P Harri Maharaj Physicist amp Head Nonmedical X Rays CCRPB Health Canada 775 Brookfield Rd Postal Locator 6301A Ottawa ON K1A 1C1 Tel 613 954 0318 Fax 613 941 1734 Email H_P_Maharaj hc sc gc ca Department of National Defence Director General Nuclear Safety Rm 1702 Standard Life Building 280 Slater Street Ottawa ON K1A 0K2 Tel 613 995 8253 Fax 613 992 5537
30. IVIS Spectrum CT Computed Tomography with in vivo and in vitro Optical Imaging User Manual October 2012 2012 PerkinElmer Health Sciences All right reserved PN 133577_Rev01 PerkinElmer Health Sciences 68 Elm Street Hopkinton MA 01748 USA Telephone 1 877 522 2447 US or 1 508 435 9500 FAX 1 508 435 3439 E mail tech support caliperls com www caliperls com Trademarks Discovery in the Living Organism IVIS Imaging System and Living Image are either registered trademarks or trademarks of Xenogen Corporation The names of companies and products mentioned herein may be the trademarks of their respective owners Apple Macintosh and QuickTime are registered trademarks of Apple Computer Inc Microsoft PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries Adobe and Illustrator are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and or other countries Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Welcome ee eee eee ee ee EUR a ee a 1 About the IVIS Spectrum CT ee eee eens 1 Optical Irnagingis s uu uu en de tee oe ea eee Wak ee etie donee ONS 2 A ray CT IMAGING dme esas dor noe eden i weld aw ceeded eae 3 3D Tomography cc 3 Caliper Technical Support 0 00 ce ee 3 Important Safety Instructions 4 DefinitlOriS sieur a pA R
31. Living Image software For more details see the Living Image Software Manual for the IVIS Spectrum CT part no 134222 IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components Figure 7 5 Field of View Examples Power 8 8X 5 0X 2 5X 1 5X FOV 22 5 13 cm 6 5 3 9 Position cm C cm cm The automatic shutter and f stop iris is integrated into the lens system A window at the top of the imaging chamber protects the lenses and other optical components in the lens compartment from contamination For more details on the care of this window see Cleaning the Lens Protection Window page 60 CAUTION Do not touch the glass window or permit specimens to come in contact with the window otherwise image quality may be impaired If the window is struck by a hard object it may crack or shatter Emission and Excitation Filters There are two 11 position filter wheels Filter settings are selected within the Living Image software For more details on how to access the filter wheels and change filters see page 60 For fluorescent studies the IVIS Spectrum CT Imaging System can illuminate a specimen from the top of the imaging chamber epi illumination or from the bottom of the stage transillumination A 12 position excitation filter wheel module is located in the back of the instrument not accessible to users If the filters must be changed or the module requires service contact IVIS Imaging Syste
32. Other user supplied chemicals or materials may be required as part of your specific biological testing procedures Do not modify the IVIS Spectrum CT in ANY manner by making any kind of hole or aperture in the instrument or removing any component of the radiation shielding 2 10 Servicing Refer all servicing to Caliper technical support If the instrument is damaged and requires service unplug the instrument from the outlet and contact Caliper technical support Servicing by anyone other than those authorized by Caliper voids any warranty covering the instrument 3 Warnings Electrical Safety X ray Safety on page 10 Mechanical Safety on page 11 Chemical and Biological Safety on page 11 Eye Safety and Burn Hazard on page 12 The IVIS Spectrum CT is equipped with a fluorescence light source module and connecting fiber optic cables that are capable of producing intense light that could be damaging to the eyes The protection means provided by the system prevents access or exposure to the fluorescent light Panels Covers and Modules on page 12 3 1 Electrical Safety A WARNING DO NOT attempt to service the IVIS Spectrum CT yourself Contact Caliper technical support for electrical service needs Although there are no voltages in excess of 45V inside the imaging chamber local line voltages can be present inside the electronics cabinets CAUTION If cleaning is necessary wipe the exterior surfaces of the IVIS Spectrum CT with
33. U doe due us bo Ue EO DO eR a VR E Rc qs Rose hes 4 Instrument Labels 2ncrza RR RR ec x RE du cad eet x Eau EE UR Re 5 IMSEFUCEHONS MONT EET EE EU TELE LLLI TERTII TITLE 5 Read Instructions sese eee hh hr 5 Retain Instructions ccc ee hh rn 6 Follow Instructions leer 6 Heed Warriings 2 360 at pex gus ex eps a Le e RC ee id as 6 X Ray Safety and Radiation Hazards Regulations o ooooooooooo 6 Fluorescence Light Source Safety llli eren 7 Environmental Consideration for System Components Ls 7 Location for the IVIS Spectrum CT llle 7 n n ai sy Gate tess oa bes el Gla a avenues aia es ons D 7 Water and Moisture 0 ccc te eee eens 7 Laboratory Space 26664 ke mm RA RR Eeke ube oe a eae RE 8 Power Considerations 0 0 cece tee hh 8 Cleaning or Moving the System Components 0000 0s ee eeeee 9 Cleaning Liquid Entry como a bee ee ed 9 Moving the IVIS Spectrum CT 0 0000 eee 9 Other Equipment or Chemicals elles 9 A a i aaa E e a a aonn iai aok WE OEA AE a E a A a ee i a er ont al E a ie a a RO EA OE E 9 PER 10 Electrical Safety o o oooooooororr ee eee hh 10 X ray Dalety 5 sae eere eos is e uos E Ge e a we dd Gwe du meshes n 10 Mechanical Safety llle 11 Chemical and Biological Safety 0 000 cee ern 11 Eye Safety and Burn Hazard ccc cc ee net eee 12 Legal Noti
34. WYOMING Office of Healthcare Licensing 6101 Yellowstone Road Suite 400 Cheyenne WY 82002 307 777 7124 http wdh state wy us main divisionsprograms html IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 78 11 4 Canadian Radiation Authorities Table 11 1 Canadian Provincial Territorial Federal Radiation Protection Contacts Non medical X rays Prince Edward Island Environmental Health Dept of Health and Social Services 16 Garfield Street PO Box 2000 Charlottetown PE C1A 2N8 Tel 902 368 4792 Fax 902 368 6468 Newfoundland and Labrador Department of Government Services West Block 4th Fl Confederation Bldg PO Box 8700 St John s NL A1B 4J6 Tel 709 729 0218 Fax 709 729 3445 New Brunswick Health Protection Branch Department of Health PO Box 5100 Fredericton NB E3B 5G8 Tel 506 453 2424 Fax 506 453 8702 Nova Scotia Occupational Health and Safety Division Department of Labour and Workforce Development PO Box 697 Halifax NS B3J 2T8 Tel 902 424 7115 Fax 902 424 5640 Quebec CSST Quebec Direction de la prevention Inspection Commission de la sante et de la securite du travail 524 rue Bourdages local 250 CP 1200 succursale Terminus Quebec OC G1K 7E2 Contact Mrs Candide Fournier T l 418 266 4699 ext 2005 IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 79 Table
35. activated using a special key provided by Caliper CAUTION If the solenoid door lock fails do not use the IVIS Spectrum CT after the solenoid is mechanically deactivated Contact Caliper technical support for a solenoid replacement page 3 Initial Steps If you are unable to open the imaging chamber door first try to disengage the lock by cycling through a few brief imaging sessions while pushing and pulling on the door handle If this fails turn the power on and off several times at the main power switch on the rear of the instrument If these attempts do not disarm the solenoid follow the steps below to mechanically deactivate the solenoid Mechanically Deactivating the Solenoid This procedure requires the Emergency Access Kit part no 133881 which includes a a 5 32 Hex driver part no 133880 Figure 9 4 Solenoid unlocking key part no 133533 54 IVIS Spectrum CT Hardware Manual Chapter 9 Troubleshooting Figure 9 4 Tools required for deactivating the solenoid lock Unlocking key 5 32 Hex driver 1 Save any data within the Living Image software then exit the program 2 Turn off the main power at the rear of the system console 3 Using the 5 32 hex driver remove the eight screws that hold the filter access panel in place on the upper right side of the console Figure 9 5 The panel is held in place by four pins until it is pulled forward Be careful not to damage the l
36. afety and the operation and safety instructions contained in this manual Caliper also recommends that personnel who operate the equipment or are in close proximity to the equipment use a radiation film badge or other type of appropriate personal dosimeter Read Instructions Read and understand all the safety and operating instructions before you install operate or perform maintenance on this product Make sure that you fully understand the following safety instructions warnings and disclaimers before proceeding to the rest of the manual IVIS Spectrum CT Hardware Manual Chapter 2 Important Safety Instructions Retain Instructions Retain the safety and operating instructions for future reference Follow Instructions Follow all operating and handling instructions Failure to follow operating or handling instructions may void any warranty covering this product Heed Warnings Abide by all warnings on the product and in the operating instructions Failure to adhere to warnings or safety precautions may void any warranty covering this product 2 4 X Ray Safety and Radiation Hazards Regulations This equipment produces X rays when energized Before operating the equipment read and understand the specific information in X ray Safety amp Radiation Hazards on page 15 WARNING DO NOT operate the IVIS Spectrum CT unless an X ray safety survey has been performed within the last 12 months For more information please contact
37. ails Power Considerations page 8 6 2 CCD Camera Optical Imaging Table 6 2 CCD camera specifications Item Description Sensor Type Back illuminated CCD Format 2048 x 2048 pixels CCD Size 26 x 26 mm Effective CCD Format 1920 x 1920 pixels Pixel Dimensions 13 5 x 13 5 um Quantum Efficiency 85 400 700nm gt 50 350 900nm Readout Noise lt 5 electrons RMS Dark Current lt 100 electrons s cm 90 C Minimum Detectable Luminance lt 70 photons s sr cm CCD Temperature Nominal 90 C IVIS Spectrum CT Hardware Manual Chapter 6 Specifications 26 6 3 Optics Table 6 3 Optics specifications Item Description Lens f stop f 1 8 Field of view FOV 4 6 5 13 19 5 22 5 cm Resolution gt 60 um FOV 3 9 f 1 6 4 X ray Source The X ray source is an Oxford Instruments Series 5000 tube with a 0 005 inch thick beryllium window and a tungsten target Table 6 4 X ray source specifications Item Description High voltage 50kV Max anode current 1mA Max anode power 50W 6 5 X ray Detector Table 6 5 X ray detector specifications Item Description Sensitive area 229 8 x 64 6 mm Resolution 3072 x 864 Pixel binning modes 1x1 2x2 4x4 pixels Mean dark current high saturation mode lt 17 electrons pixel second at 40 C Mean dark current high sensitivity mode lt 17 electrons pixel second
38. aphy 808 586 4700 http hawaii gov health environmental noise radiationsection radiation html IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information Table 11 2 US Radiation Authorities continued IDAHO Dept of Health amp Welfare Idaho Bureau of Laboratories 2220 Old Penitentiary Rd Boise ID 83712 8299 208 334 2235 ext 245 http healthandwelfare idaho gov Health Labs Certification tabid 186 Default aspx ILLINOIS IL Emergency Management Agency Division of Nuclear Safety 1035 Outer Park Dr Springfield IL 62704 217 785 9868 Registration and Certification Section 217 785 6982 Fax 217 785 9946 http www iema illinois gov iema dns asp INDIANA State Department of Health Epidemiology Resource Center Indoor and Radiological Health 2525 North Shadeland Avenue E3 Indianapolis IN 46219 317 351 7190 Ext 257 http www in gov isdh 23279 htm IOWA Bureau of Radiological Health Lucas State Office Bldg 5th Fl 321 E 12th St Des Moines IA 50309 4611 515 281 3478 http www idph state ia us eh radiological health asp KANSAS Radiation Section 1000 SW Jackson St Suite 310 Topeka KS 66612 1366 785 296 1565 http www kdheks gov radiation indexXray html KENTUCKY Radiation Control Program Cabinet for Health amp Family Services 275 East Main Street HS1C A Frankfort KY 40621 0001 502 564 3700 Ext 3695 http chfs ky gov d
39. asing dose Stochastic Effects Stochastic effects are biological effects that have a statistical probability of occurring based on the radiation dose Unlike deterministic effects stochastic effects have no dose threshold Even for low radiation doses there is a small probability of a biological effect occurring The severity of the stochastic biological effect can be unrelated to the magnitude of the dose but the probability of occurrence increases with increased dose or length of exposure Stochastic effects cannot be linked to a specific radiation exposure with certainty For example stochastic effects such as cancer can also occur in individuals who have not been exposed to radiation above background levels therefore it is not possible to determine that the cancer resulted from any specific exposure X ray Dose Limits The IVIS Spectrum CT has been tested at maximum operating conditions and Caliper has determined that the local X ray dose rate at a distance of 5 cm from the surface of the equipment is less than 1 0 uSv h Caliper declares that the IVIS Spectrum CT system conforms to a 1996 29 Euratom Directive Dose rate of 1 uSv h at 10 cm from any accessible surface under normal operating conditions US CFR21 Part 1020 40 Regulation Dose rate of 0 5mrem h at 5cm outside of the external surface under maximum operating conditions in accordance with the following standard IEC 61010 1 2001 Standard Dose limit of 1 uSv h at
40. at 40 C MTF gt 40 at 3 Lp mm gt 10 at 6 Lp mm System noise 7 5 analog to digital units Linearity high sensitivity mode 4 Linearity high saturation mode 3 IVIS Spectrum CT Hardware Manual Chapter 6 Specifications 27 6 6 Fluorescent Imaging Components Table 6 6 Fluorescent imaging components specifications Item Description Excitation filters 12 positions 25 mm diameter 11 ND2 filters supplied standard Emission filters 22 positions 60 mm diameter 18 filters supplied standard Background autofluorescence and leakage 1 part in 107 typical Lamp 150W quartz halogen 21VDC 3250 Kelvin 6 7 Environmental Temperature and Humidity Table 6 7 Environmental temperature and humidity specifications Item Description Temperature 18 24 C 65 75 F Humidity 0 8096 non condensing Type of use Indoor Pollution Decree 2 Sound level 61 dB 500 Hz Stage temperature Ambient to 40 C Altitude rating 2000 meters 6560 ft 6 8 Weight and Dimensions Table 6 8 Weight and dimension specifications Item Description Weight 334 kg 735 Ibs Depth 77 cm 30 in Width 65 cm 25 5 in Height 206 cm 77 in Imaging chamber internal dimensions 51x 51x 66 cm Dx Wx H 7 ise Spectrum CT Components CCD Camera for Optical Imaging on page 29 X ray Source Detector and Filters on page 30 X ray System Control Panel on pag
41. ble 11 1 Ordering information Item Part No Black Paper Swarthmore Artagain 9x12 24 Sheet pad 117837 Kit Foam Animal Beds Single Mouse 3mm holes set of 10 134371 Kit Foam Animal Beds and Light Baffles Dual Mouse Manifold set of 10 134372 IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 68 Table 11 1 Ordering information continued Item Part No Kit Animal Bed Substrate 2mm holes with Lamination Instructions 134377 Light Baffle Foam Dual Mouse Manifold 134151 Kit Tools for Emergency Access to Spectrum CT Imaging Chamber 133881 Low Fluorescence Imaging Mats 11 6 square set of 10 119000 Mouse Inhalation Nose Cones for 5 Port Manifold set of 10 119001 Light Baffle 0 5 High single 117151 Light Baffle 0 7 High single 117152 XRS 10 Rubber Stoppers for 5 Mouse Manifold set of 10 119006 XNC LP Rat Nose Cones 5 Port Manifold For FOV B C and D only 119025 Optical Lens Cleaner 123495 Wipe Lint Free 50 package 126291 Phantom Mouse XFM 2 CT FLIT 133803 XRM 5 Phantom Mouse XRM 5X Ray simulates fat inner bone cortical bone solid water 133793 Assembly Phantom Mouse XPM 2 CT DLIT 133805 XLS 4 Calibrated Light Source 118897 XRH 1 High Reflectance Hemisphere with Container 118937 XGI 8 Anesthesia System 120Volt 118918 XGI 8 Anesthesia System 230Volt 118919 XGI 8 Anesth
42. c or magnetic fields exist No vibrations are present No corrosive gases are present High amounts of dust are not present No open flame is present There is sufficient space behind the IVIS Spectrum CT equipment A minimum space of six inches 15 cm from the flat surface of the rear panel should be provided behind the IVIS Spectrum CT to provide unobstructed air flow and access to the main power on off switch The floor is level and structurally sound Heat The system should be situated away from heat sources such as open flames radiators heat registers stoves and other heat generating electrical equipment Water and Moisture VOLTAGE Do not use the IVIS Spectrum CT near water for example near a sink or wet room due to the risk of electric shock electrical damage and or system failure IVIS Spectrum CT Hardware Manual Chapter 2 Important Safety Instructions Laboratory Space The IVIS Spectrum CT requires a minimum of 100 square feet 9 square meters of laboratory space for adequate ventilation At least of six inches of space is required behind the rear flat panel to provide unobstructed air flow If you will be using an XGI 8 Gas Anesthesia System or other anesthesia system refer to the anesthesia system manual for additional ventilation requirements to avoid exposure to waste anesthesia gases Allocate an area that is at least 36 inches 91 4 cm wide and 51 inches 129 5 cm deep for the instrumen
43. camera from overexposure to light during optical imaging A redundant switch with a removable copper rod cuts all power to the X ray source should the door be opened and a solenoid switch failure occurs in the electrically ON state There are several other interlocks that protect the user from X ray exposure when one or more of the electronic access panels are removed The filter wheel access panel is interlocked The interlock switch will break the X ray power circuit if the user removes this panel for filter replacement or when IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 39 using the emergency imaging chamber access procedure described in Chapter 9 page 54 Two safety interlock switches protect the electronics bay on the right side of the instrument and the upper rear panel which encloses the X ray source These panels should only be removed by a Caliper service technician 7 11 Base Enclosure IMPORTANT Do not block the ventilation openings on the base of the IVIS Spectrum CT or position the instrument too close to a wall so that the exhaust flow is obstructed The base is equipped with four casters that enable you to move the instrument Figure 7 11 For more details on how to move the IVIS Spectrum CT see page 65 Figure 7 11 Instrument base with casters Locking casters on the instrument right side 7 12 Acquisition Computer The IVIS Spectrum CT Imaging System includes a W
44. cated on the imaging platform where it exits through the port labeled GAS Three mouse anesthesia manifolds are provided with the IVIS Spectrum CT See Table 7 1 on page 34 for more details on these CAUTION Use only the manifolds provided with the IVIS Spectrum CT Using other manifolds may cause damage to the instrument Some manifolds for other Caliper imaging systems are taller and will break the lens window if the imaging platform is moved to Field of View A The manifold port labeled GAS connects to the GAS port on the platform distribution block The anesthesia manifold also has a port labeled EXH that should be connected to the EXH port on the platform distribution block The vacuum or exhaust circuit connects to the GAS OUT port on the rear of the system console No gases are exhausted from the imaging chamber unless gas scavenging equipment such as the Caliper XGI 8 Gas Anesthesia System is used 8 4 Door Operation and Interlocks WARNING Never try to defeat the door interlock or force the door open during image acquisition when the status light is red or X rays are being generated Such action could expose the user to a severe pinch hazard from the rapidly moving imaging platform or dangerous levels of X rays After the system has been initialized the door of the imaging chamber cannot be opened when the system status light is red The door is equipped with a solenoid lock to prevent accidental opening during an imaging
45. ces 202 200 200 ee ee eee eee ERR 13 Limited Warranty 1 1 0 0 0c eee eee eee eas 13 Patents anti ee bitte S3 e ee tate HE EIE UE een B aire Sn a RES ITE tte ea ete 14 Trademarks nic e Roe a ale ve da 14 Chapter 5 Chapter 6 Chapter 7 Contents ii X ray Safety and Radiation Hazards 15 Introduction iius Rem ok dos mado Ro A RR E NUR ACA RR COR RR a eon E 15 Product Documentation lees 15 Radiation Theory and X ray Generation eee eens 16 X rays lonizing Radiation 000 0 ee es 16 X rays Penetrating Radiation llle 16 How X rays are Generated 0 eee eee ee 16 IVIS Spectrum CT X ray Source Tube 0 eee ees 17 Biological Effects of Radiation 0 0 0 ee 17 Deterministic Effects sui cesse uu be ee eee eee 18 Stochastic Effects i eal eteti Cees eae Bea as aS nw a eo A a nc 18 X ray Dose LIMIS 0 ccc eee hs 18 IVIS Spectrum CT Safety Systems 0 ccc eee eas 19 Radiation Shielding 0 0 00 cece ee 19 The IVIS Spectrum CT Control Panel 00 000 eee ee 19 Requirements for Turning on X rays ooccoococoo ee 20 Safety Interlocks 2 0 ccc ee hn 21 Regulatory Compliance and Laboratory X ray Safety Procedures 23 Contact the State or Provincial Radiation Protection Authority 23 Study Documentation 0 0 eee ee eee ees 23 Create a Radiation Safety Plan
46. ch as the creation of free radicals or other ions The deleterious effects of radiation exposure are classified into two categories deterministic effects and stochastic effects IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards 18 Deterministic Effects Deterministic effects are those effects in which a clear causal connection can be made between the exposure to radiation and the effect Deterministic effects are the result of cell killing and tissue damage This effect is dose related a radiation dose greater than a certain threshold must occur which produces enough cell death to result in tissue damage After the dose threshold is exceeded the severity of the effect is increased by the amount of the dose Examples of deterministic effects of radiation due to overexposure of X rays include skin changes reddening pigmentation changes blistering and ulceration cataract formation and fetal abnormalities due to exposure in utero The deterministic effects of radiation can be classified as either acute or delayed An acute effect such as skin reddening occurs soon after overexposure to radiation whereas a delayed affect such as cataracts may take some time even years to develop For deterministic effects there is a clear connection between the individual exposure to radiation and the biological effect The biological effect requires a minimum threshold dose and the severity of the effect increases with incre
47. ctrum CT is equipped with a fluorescence light source module and connecting fiber optic cables that are capable of producing intense light that could be damaging to the eyes The protection means provided by the system prevents access or exposure to the fluorescent light Panels Covers and Modules Aside from the imaging chamber door the filter wheel access panel and the lower rear cart panel are the only user removable panels The lower rear cart panel allows access to the chiller for coolant filling There are no user serviceable components in the electronics cabinets of the IVIS Spectrum CT Do not remove the electronics cabinet covers from the instrument unless instructed by and under the supervision of Caliper technical support Do not modify the IVIS Spectrum CT in ANY manner by making any kind of hole or aperture in the instrument Do not remove any component that is part of the radiation shielding In certain situations the Emission Filter Wheel Access Panel may be removed see page 60 4 Legal Notices Limited Warranty Patents on page 14 Trademarks on page 14 4 1 Limited Warranty Caliper Life Sciences Inc Caliper provides the following limited warranty for each new IVIS Spectrum CT System as follows Limited Warranty This Limited Warranty for the System extends to the original purchaser Customer for a period of one 1 year following installation of the System and is not assignable or transferable to any s
48. dering Information 73 Table 11 2 US Radiation Authorities continued MISSISSIPPI Division of Radiological Health State Department of Health 3150 Lawson Street Jackson MS 39215 1700 601 987 6893 http www msdh state ms us msdhsite _static 30 0 102 html MISSOURI Medical Radiation Control Program Health Services Regulation Division of Regulation and Licensure PO Box 570 Jefferson City MO 65102 0570 573 751 6083 http www dhss mo gov RadProtection MONTANA Radiological Health Program MT Dept of Public Health and Human Services Licensure Bureau P O Box 202953 Helena MT 59620 2953 406 444 2868 https app mt gov radio NEBRASKA Office of Radiological Health Dept of Health amp Human Services P O Box 95026 Lincoln NE 68509 5026 402 471 0528 www dhhs ne gov rad NEVADA Radiological Health Section Bureau of Health Protection Services Nevada State Health Division 4510 Technology Way Suite 300 Carson City NV 89706 775 687 7540 http health nv gov HCOC Radiological htm NEW HAMPSHIRE Radiological Health Section Division of Public Health Services Dept of Health and Human Services 29 Hazen Drive Concord NH 03301 6504 603 271 4585 http www dhhs nh gov DHHS RADHEALTH default htm IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information Table 11 2 US Radiation Authorities continued NEW JERSEY Radiatio
49. duced by radioactive materials However since the IVIS Spectrum CT contains no radioactive materials there is no possibility of transferring a radioactive contaminate from the instrument to the laboratory during a procedure such as cleaning the imaging chamber X rays Penetrating Radiation The ability of X rays to penetrate matter makes them useful in applications such as medical imaging and industrial inspection The IVIS Spectrum CT uses an X ray tube source with the minimum energy required to generate X rays that penetrate and image mice Even though X rays are able to penetrate matter such as tissue or plastic they are not able to make matter radioactive How X rays are Generated The IVIS Spectrum CT is a computed tomography CT imaging system that consists of a fixed X ray source which produces a cone beam of X rays The shelf consists of the main platform and a rotating animal stage For CT imaging the platform moves down into the bottom of the instrument During CT Imaging the animal stage rotates about the vertical axis while the fixed horizontal beam IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards 17 p of X rays penetrates the animal The X ray source is in the behind the rear of the imaging chamber and the X ray detector is in the front The X ray source is an X ray tube see page 17 for more details on the tube characteristics its basic function is to generate X rays of a maximum energy of 50
50. e 30 Key Selector Switch and Lost Keys on page 31 Thermoelectric Chiller on page 31 Imaging Chamber on page 32 Optical Components on page 32 Specimen Warming System on page 34 Rotating Stage and Anesthesia Connections on page 34 X ray Door and Panel Interlocks on page 38 Base Enclosure on page 39 Acquisition Computer on page 39 High Reflectance Hemisphere on page 39 The IVIS Spectrum CT includes the following components a Scientific imaging charged coupled device CCD camera Imaging chamber with specimen warming system Thermoelectric camera cooling system A pattern illuminator for animal positioning a System of lenses and filters for bioluminescent image acquisition Photographic illumination system a Integrated fluorescence system for fluorescent image acquisition An X ray generating source X ray detector and rotating animal stage for CT imaging These components are integrated into one freestanding instrument that is equipped with casters Figure 7 1 IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 29 Figure 7 1 IVIS Spectrum CT Imaging System A pre configured Windows based computer enables you to control the system as well as acquire and analyze images IMPORTANT If you modify the IVIS Imaging System in any way without prior approval from Caliper all warranties that cover this product are void In addition the computer included with the IVIS Spectrum CT
51. ection in the United States and Canada IVIS Spectrum CT Hardware Manual Provides information about safe operation of the IVIS 133577 Spectrum CT system components care and maintenance of the system and spare parts Living Image Software Manual forthe Provides instructions for image acquisition on the IVIS 134222 IVIS Spectrum CT Spectrum CT and image analysis using the Living Image software IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards 16 5 2 Radiation Theory and X ray Generation Radiation is everywhere Our bodies are continuously bathed in radiation in the form of sunlight radio and television waves as well as radiation produced by the earth s natural background radiation that is produced by radioactivity and cosmic rays Radiation is distinguished by its ability to ionize chemical bonds and is characterized as either ionizing or non ionizing Ionizing radiation has the ability to affect biological organisms including human beings by interacting with cellular chemistry Radiation such as sunlight is usually considered non ionizing radiation although there is some overlap when discussing ultra violet radiation X rays are a form of electromagnetic radiation similar to light however X rays have much shorter wavelengths X rays have wavelengths from 10 to 0 01 nanometers whereas visible light ranges from 700 to 400 nanometers Non visible ultraviolet light fills the gap between vi
52. ept of Health Services PO Box 14937 Austin TX 78714 9347 512 834 6679 http www dshs state tx us radiation UTAH Division of Radiation Control 168 North 1950 West PO Box 144850 Salt Lake City UT 84114 4850 801 536 4257 http www radiationcontrol utah gov XRAY reginspc htm VERMONT Office of Radiologic Health Department of Health 108 Cherry Street PO Box 70 Burlington VT 05402 802 865 7730 http healthvermont gov enviro rad rad_health aspx VIRGINIA Division of Radiological Health Department of Health James Madison Bldg 109 Governor Street Room 730 Richmond VA 23219 804 864 8170 http www vdh state va us epidemiology RadiologicalHealth WASHINGTON Office of Radiation Protection Department of Health PO Box 47827 Olympia WA 98504 7827 360 236 3210 http www doh wa gov ehp rp xray reg htm IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 77 Table 11 2 US Radiation Authorities continued WEST VIRGINIA Radiological Health Program Office of Environmental Health Services DHHR Bureau for Public Health 1 Davis Square Suite 200 Charleston WV 25301 1798 304 558 6721 http www wvdhhr org rtia radiological health asp WISCONSIN Radiation Protection Section Dept of Health and Family Services PO Box 2659 Madison WI 53701 2659 608 267 4792 dhfs wisconsin gov dph beh RadiatioP Index htm
53. er rod is permanently mounted on the door When the door is opened part of the electrical circuit is removed from the X ray generating circuit and contact is lost between the two flexible contacts in the switch body This switch is sometimes referred to as a CDRH switch and meets US FDA requirements as well as some other international regulations Figure 5 4 Second redundant safety interlock switch Copper rod engaged Copper rod disengaged 22 IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards 23 p 5 5 Regulatory Compliance and Laboratory X ray Safety Procedures Aside from what Caliper has done to make a safe system there are some things that are required of the user to ensure that the instrument operates safely and within legal authority Contact the State or Provincial Radiation Protection Authority You must contact your state or provincial radiation protection authority before operating the IVIS Spectrum CT Contact information is provided in Safe Operating and Emergency Procedures for the operation of the Spectrum CT Cabinet X ray System PN 133919 This document was provided at the time of ordering it is also included on the IVIS Spectrum CT Hardware Manual CD as well as the CD governing international documentation Chapter 11 also provides contact information for United States radiation authorities page 69 and Canadian Provincial radiation authorities page 78 In most cases
54. esia System 100Volt 118957 XPM 2 Bioluminescent Phantom Mouse 118993 XWS 248 Workbench 48 wide 119208 XWS 260 Workbench 60 wide 119207 XWS 272 Workbench 72 wide 119212 Kit Mouse Imaging Shuttle Spectrum CT 134366 Kit Animal Bed Lamination Flxture and Instructions 134375 Kit Rotation Stage Alignment measures shelf pitch and yaw alignment and Instructions CLS135992 IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 69 11 3 US Radiation Authorities Table 11 2 US Radiation Authorities ALABAMA Office of Radiation Control State Dept of Public Health 201 Monroe St PO Box 303017 Montgomery AL 36130 3017 334 206 5391 http www adph org radiation ALASKA Radiologic Health Program 4500 Boniface Parkway Anchorage AK 99507 1270 907 334 2107 http www hss state ak us dph labs radiological ARIZONA Arizona Radiation Regulatory Agency 4814 South 40th St Phoenix AZ 85040 2940 602 255 4845 Ext 222 http www arra state az us ARKANSAS Arkansas Department of Health Radiation Control Section 4815 W Markham St Slot H 30 Little Rock AR 72205 3867 Phone 501 661 2301 www healthyarkansas com rtl CALIFORNIA Radiologic Health Branch Division of Food amp Radiation Safety PO Box 997414 MS 7610 Sacramento CA 95899 7414 916 440 7899 http www cdph ca gov programs Pages RadiologicHealthBranch aspx COLORADO X Ray amp Mammo
55. f incidental or consequential damages for certain products or the limitation of liability for personal injury so the above limitations and exclusions may be limited in their application to you When any implied warranties are not allowed to be excluded in their entirety they will be limited to the duration of the applicable written warranty This Limited Warranty gives you specific legal rights which may vary depending on local law vii This Limited Warranty shall be governed by the laws of the State of California U S A excluding its conflicts of laws principles and excluding the United Nations Convention on Contracts for the International Sale of Goods 4 2 Patents The detection and imaging of light originating within mammals is the subject of several issued patents and pending patent applications in the United States and around the world including U S Patent Numbers 5 6200 135 6 217 847 6 649 143 6 890 515 and European Patent Commission Number EP0861093 for which Xenogen Corporation is the exclusive licensor The use of an IVIS Imaging System for such applications requires a sublicense from Xenogen Corporation In addition many of the hardware and software components of the Imaging System are the subject of various issued patents and pending patent applications owned by Xenogen including United States Patent Number 6 614 452 Graphical User Interface for In Vivo Imaging and 6 775 567 Improved Imaging Apparatus and United State
56. graphy Compliance Registration amp Certification 4300 Cherry Creek Drive South Denver CO 80246 1530 303 692 3446 http www cdphe state co us hm rad xray IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 70 Table 11 2 US Radiation Authorities continued CONNECTICUT Dept of Public Health Division of Radiation 79 Elm Street Hartford CT 06106 5127 860 424 3029 http www dep state ct us DELAWARE Office of Radiation Control Division of Public Health 417 Federal Street Dover DE 19903 302 744 4546 http www dhss delaware gov dph hsp orc html DISTRICT OF COLUMBIA Department of Health HRLA Radiation Protection Div 717 14th Street NW Room 639 Washington DC 20005 202 724 8800 http hrla doh dc gov hrla site default asp FLORIDA Dept of Health Bureau of Radiation Control 4052 Bald Cypress Way Bin C21 Tallahassee FL 32399 1741 850 245 4266 Radiologic Technology Program 4052 Bald Cypress Way Tallahassee FL 32399 1741 850 245 4540 www doh state fl us environment radiation GEORGIA Department of Human Resources 2 Peachtree Street NW 33rd Floor Atlanta GA 30303 3142 404 657 5400 http ors dhr georgia gov portal site DHR ORS HAWAII Dept of Health Noise amp Radiation Branch 591 Ala Moana Blvd Honolulu HI 96813 4921 808 586 4700 Radiation Section Radiological Response Radiologic Technology Mammogr
57. ight sealing gasket when removing the panel Figure 9 5 Filter wheel access panel Machined recess The filter access panel has a machined recess on the inner left hand side towards the front of the console Noting this orientation is helpful when you reinstall the panel Figure 9 5 4 Disconnect the electrical connector towards the front of the IVIS Spectrum CT by rotating the knurled ring counter clockwise Figure 9 6 5 After the electrical connector is removed locate the triangle shaped rotating key receptacle One vertex of the triangle has a small dot that points to LOCK Figure 9 7 55 IVIS Spectrum CT Hardware Manual Chapter 9 Troubleshooting Figure 9 6 Disconnecting the electrical connector and inserting the unlocking key into the key receptacle Disconnect the electrical connector to access the Insert the unlocking key through the aperture in key receptacle the sheet metal Figure 9 7 Key receptacle and solenoid unlocking key Dot on the key Key receptacle in Unlocking key inserted into the key receptacle receptacle the LOCK position 8 9 Insert the unlocking key into the triangular receptacle and turn the key counter clockwise to so that the dot on the key receptacle points to the UNLOCK position Figure 9 7 The solenoid will mechanically disengage Open the door and remove the subject s from the imaging chamber Using the
58. in the closed position turn the carriage locking clamp in the clock wise direction Be careful not to dislodge any drive belts off of their pulleys when locking the clamp 12 Replace the access panel by positioning it onto the four pins The inner machined out recess should be on your left side 13 Loosely install the eight screws then lightly tighten the four central screws and in a crisscross pattern Lightly tighten the four outer screws in a crisscross pattern D NOTE Do not over tighten the screws The panel is designed to seal out light without large clamping force 14 Restart the system following the procedure on on page 43 10 5 Servicing the Chiller Unit The coolant level in the chiller unit may diminish over time and require replenishing It is recommended that you check the coolant level in the chiller unit every six months This section explains how to prepare and add coolant to the chiller unit Required Items The following tools and items are required but not provided Allen Wrench hex head wrench 3 32 measured across the flats AF A disinfecting coolant mixture of 25 pure ethanol and 75 distilled water To prepare 750 ml Mix 200 ml of 190 proof ethanol and 550 ml of distilled water If pure ethanol is not available a suitable substitute can be made from ordinary 80 proof vodka and distilled water To prepare 750 ml of disinfecting coolant Mix 470 ml of 80 proof vodka 40 ethanol and 280 ml
59. indows based computer The Living Image software and Microsoft Office software are installed on the acquisition computer The Living Image software controls the IVIS Spectrum CT and displays and analyzes the image data A 20 inch monitor and a 10 100 Ethernet network adapter card are also included with the imaging system A printer may be attached to the computer 7 13 High Reflectance Hemisphere The optional High Reflectance Hemisphere is used to check for light contamination in the imaging chamber Figure 7 12 The hemisphere does not emit any photons when it is imaged in a light tight and contaminant free imaging chamber As a result it is not visible in a luminescent image If a light leak or an internal phosphorescent contaminant is present in the imaging chamber the hemisphere reflects that light and is visible in a luminescent image If you suspect light contamination in the imaging chamber see Internal light contamination in Chapter 9 page 51 A light leak test is also a useful check for radiation leakage If light can get into the imaging chamber it is possible that X rays could escape IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 40 Figure 7 12 High Reflectance Hemisphere 8 Operating the IVIS Spectrum CT Starting the IVIS Spectrum CT Restarting the System After a Power Outage on page 43 Gas Plumbing on page 43 Door Operation and Interlocks on page 44 Centering Subject
60. ion and allow the knob to pop out Restart the IVIS Spectrum CT following the procedure in Restarting the System After a Power Outage on page 43 48 9 Troubleshooting Measured Temperature is not Equal to the Demand Temperature Photographic Image is Unacceptable Luminescent Image is Unacceptable on page 51 No Optical Image is Produced on page 51 No CT Image is Produced on page 52 Hardware Problems on page 52 Accessing the Area Under the Imaging Platform on page 53 Imaging Chamber Emergency Access on page 54 9 1 Measured Temperature is not Equal to the Demand Temperature At start up the Living Image software programs the CCD camera to maintain the CCD temperature at 90 C To check the temperature of the CCD click the Temperature square red or green in the Camera Control Panel of the Living Image software Figure 9 1 Figure 9 1 IVIS Acquisition Control Panel Subject height 1 50 Click the temperature box to view the demand and measured temperatures of the CCD camera and stage Temperature box color indicates System not initialized System is initialized and CCD camera is at or within acceptable range of the demand temperature and locked The system is ready for imaging System Status X Rays will be produced when energized System is initialized but CCD camera Acquire temperature is out of range and not Imaging Wizard ready for imaging 3 9 2 Ph
61. ipe away any liquid from the top or walls of the chiller c Close the chiller cap 64 IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance 65 Figure 10 6 Filling the chiller with coolant 6 Return the chiller to the tray and slide the tray back into the console while holding hoses and cables out of the way to prevent snagging 7 After the chiller is mounted in position confirm that The chiller power switch is on and no tools or other hardware remain inside the instrument The cap is secure a No fluids have spilled onto the chiller or the Spectrum CT pull out shelf 8 If any fluid is present wipe it off before proceeding to the next step Replace the lower rear panel using a 3 32 hex head wrench to tighten the screws Figure 10 4 on page 63 9 Restart the IVIS Spectrum CT Confirm that the instrument rear panel power switch is OFF Reconnect the power cord first to the console and then to the wall socket Confirm that all other cables are properly connected Turn on the computer Turn the rear panel power switch ON moan ol TJ os Start the Living Image software and initialize the system The status light is red and turns green when the system is initialized Wait until the camera has cooled to the demand temperature before you begin an imaging session approximately 10 20 minutes 10 6 Moving the System Removing the Dome It may be necessary to remove the plas
62. is specifically configured to run all system related applications Any modification of existing software or hardware voids all warranties If you have any questions please contact Caliper technical support 7 1 CCD Camera for Optical Imaging The camera is a scientific grade thermoelectrically cooled back thinned back illuminated large format CCD manufactured for Caliper The camera uses a thermoelectric water chiller Two flexible plastic water lines connect the CCD camera to the thermoelectric chiller unit The CCD camera has a heated vacuum sealing window to prevent condensation that can obscure the image or damage other components The camera power supply is housed in the base of the instrument and maintains the camera operating temperature when the computer is turned off It has a 16 bit digitized low noise electronic readout for extremely low background images IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 30 7 2 X ray Source Detector and Filters During CT Imaging the animal stage moves to the lower compartment of the imaging chamber The animal stage rotates while X rays are generated from the X ray source located at the rear of the IVIS Spectrum CT Figure 7 2 A series of images are collected by the detector located at the front ofthe instrument During the brief period when the X ray tube is warming up to its maximum voltage and current a blocking filter is positioned so that the animal
63. isoflurane with the IVIS Spectrum CT DO NOT USE FLAMMABLE ANESTHESIA GAS CAUTION Caliper recommends using the XGI 8 Gas Anesthesia System when imaging small animals Figure 1 1 on page 2 This system supplies a controlled amount of isoflurane to the imaging chamber and continuously reduces the build up of isoflurane in the chamber If you plan to use a gas other than the recommended isoflurane oxygen gas mixture or pure air contact IVIS technical support Be careful to use only tubing and other plumbing fixtures that do not fluoresce or phosphoresce glow in the imaging chamber Please contact IVIS technology support for a list of acceptable materials The IVIS Spectrum CT is equipped with plumbing connections for supplying isoflurane anesthetic gas to the imaging chamber and exhausting gases out The flow of gas into the imaging chamber is controlled by the gas valve on the front panel of the XGI 8 The imaging chamber does not include a gas scavenging system This is provided by suitable auxiliary equipment such as the XGI 8 Gas Anesthesia System Gas ports are located on the rear of the system console and are labeled GAS IN and GAS OUT IVIS Spectrum CT Hardware Manual Chapter 8 Operating the IVIS Spectrum CT 44 a GAS IN white The direction of flow is into this port a GAS OUT yellow The flow is exhausted out of this port The flow of gas from the rear panel GAS IN port is directed to a distribution block lo
64. key return the solenoid to the LOCK position by fully turning the key in the clockwise direction Reinstall the electrical connector Make sure that it is fully engaged 10 Replace the filter wheel access panel CAUTION Do not use the IVIS Spectrum CT after mechanically deactivating the solenoid lock Contact Caliper technical support for a solenoid replacement page 3 56 1 0 Care and Maintenance Surveying the IVIS Spectrum CT for Radiation Leakage Maintenance and Safety Checks Cleaning the IVIS Spectrum CT on page 58 Changing the Emission Filters for Fluorescence Imaging on page 60 Servicing the Chiller Unit on page 62 Moving the System on page 65 10 1 Surveying the IVIS Spectrum CT for Radiation Leakage Caliper recommends and some local government agencies may require that you perform an X ray leakage safety test under the following conditions Every 12 months When the system is installed at a new site requiring truck transport Moving the IVIS Spectrum CT on its casters should not require a re survey a After Caliper performs maintenance or service a After any abnormal condition that could impair any of the safety systems For example if the imaging chamber door becomes difficult to open or close Conducting the X Ray Radiation Survey A radiation leakage test is a complicated matter requiring sensitive and expensive equipment Some states or localities may require special training and certification
65. ks lees 38 Base Enclosure i053 cei dated bet a xc oh E back RUE Quse aod Rcs Rc a de e ies 39 Acquisition Computer seris panagan RR ERR sede de eee dae AER UR RR RAE 39 High Reflectance Hemisphere llle eene 39 Operating the IVIS Spectrum CT 41 Starting the IVIS Spectrum CT 0 0 00 000 es 41 Restarting the System After a Power Outage 00000 2c eee enn 43 Gas PIUMDING sese mane PRrtstededededadvaug ake e a ae ge msde aed 43 Door Operation and Interlocks 0 0c cece ee eee ee eas 44 Centering Subjects in the Field of View llle 44 Optical Imaging Basics osseuse naani wa aa ua OR E A E E A a 45 Black Paper s 2 22922 92999 ee ee oa E yer A oe en nee 46 Low Fluorescence Mat 00 eee eee 46 Glowing Materials warina pitaa eae he dene Sees Eade eee EORR eed 47 CT Imaging Basics 0 eee ee 47 Normal System Shut Down Procedure eee ees 48 Emergency System Shutdown Procedure 00 cece eee eee eens 48 Troubleshooting 2 2226 0888ce eee e ee eee eee ee ee sa 49 Measured Temperature is not Equal to the Demand Temperature 49 Photographic Image is Unacceptable 00 cc cece eee ees 49 Luminescent Image is Unacceptable ococccccccccc ee 51 No Optical Image is Produced 000000 e ee ee 51 No CT Image is Produced 000 c cee eee 52 Hardware Problems cse hh
66. ld in the photograph An excessively moist environment in the imaging chamber can result in condensation on the CCD window Figure 9 2 A white spot appears in the center of the field of view Remove excess moisture in the imaging chamber and allow it to thoroughly dry Place a desiccant in the imaging chamber to decrease the drying time Do not touch or let specimens touch the window at the top of the imaging chamber For more details on the care of this window see page 60 If the problem persists contact Caliper technical support for assistance Figure 9 2 Example photograph showing the result of condensation on the CCD window 50 IVIS Spectrum CT Hardware Manual Chapter 9 Troubleshooting 9 3 Luminescent Image is Unacceptable Binning f stop and exposure time affect the appearance of a luminescent image Please see the Living Image Software Manual for instructions on setting binning exposure time and f stop values In order to function properly and reduce camera noise the CCD camera must be cooled to the demand temperature before acquiring an image If the camera is not cooled to the demand temperature imaging may result in false positive signals Table 9 2 Troubleshooting luminescent images Problem Corrective Action Camera noise Verify that the camera is cooled to the demand temperature 1 Check the measured temperature in the Camera Control panel to ensure that it is
67. lectronic circuits are located within the interlocked shielded cabinet Only Caliper Field Service personnel are allowed to remove the access panel as it requires the use of a special tool to remove the tamper proof screws D NOTE The only user accessible door and panels are the imaging chamber door the filter access panel see page 60 and the lower rear cart panel that provides access to the thermoelectric chiller see page 62 There are no other panels that can be removed or opened by the user The tube is rated at SOKV high voltage potential with a maximum beam current of I milliAmp Total power is 50 0 watts The X ray window is 0 13 mm 0 005 inches thick beryllium and the X ray target is tungsten The X ray tube controls including ON OFF and beam power settings are carried out by Spectrum CT software commands which limit the X ray tube voltage to 50kV maximum 5 3 Biological Effects of Radiation X rays are a form of electromagnetic radiation that has enough penetrating energy to ionize atoms within a cell Ionization occurs when an X ray photon interacts with an orbital electron and transfers energy to it causing the electron to be ejected from the atom Such ionizations may disrupt molecules such as DNA The DNA molecule can be broken by the radiation and the cell can be severely damaged resulting in cell death With enough cell death tissue and organs can be damaged Injury to a living organism can also occur in indirect ways su
68. lized to density p and then represented as the mass attenuation coefficient u p Therefore materials which are large and dense can result in artifacts Furthermore materials with a high atomic number and or high density for example stainless steel can also create artifacts To minimize all of these potential pitfalls a Stabilize the animal as much as possible Image only biological tissues a Avoid metal including ear tags Select CT mode in the IVIS Acquisition Control Panel to perform CT imaging or include CT imaging with luminescent or fluorescent imaging Figure 8 4 Figure 8 5 Control panel in the Living Image software Field of View X Rays will be produced when energized l Service 13 5 cm Wizard Subject height 1 50 on Sequence Setup Focus use subjectheight y Temperature E Locked Field of View System Status X Rays willbe produced COCA i energiz e when ene a Subject height 150 om it Sequence Setup Focus use subjectheight y Temperature AE Locked CT imaging mode Luminescent and CT imaging mode IVIS Spectrum CT Hardware Manual Chapter 8 Operating the IVIS Spectrum CT 8 8 Normal System Shut Down Procedure Caliper does not recommend power cycling the IVIS Spectrum CT turning the system components off and on on a daily basis Leaving the system on keeps the camera cold and the electronics systems stable The software is preconfigured to ru
69. locked If the camera temperature is locked the camera temperature box is green 2 If the camera temperature box is red click the red box to display the actual temperature See Weasured Temperature is not Equal to the Demand Temperature page 49 Internal light Check to see that there are no extraneous light sources inside the imaging chamber contamination Many substances phosphoresce when exposed to light Be especially cautious of plastics and substances that contain pigment Be sure to prescreen any substance or material before performing actual experiments External light A 2 diameter High Reflectance Hemisphere Figure 7 11 on page 39 is available contamination from Caliper to help check for light leaks XRH 1 Caliper part no 118937 To check for light leaks 3 Place the high reflectance hemisphere in the imaging chamber on the stage using a subject height of 3 5 cm at field of view D 4 Take a luminescent image of the hemisphere using the luminescent settings f stop 1 Binning Large high sensitivity and exposure time 5 minutes If the hemisphere can be easily seen there is a light leak Contact Caliper technical support for assistance A WARNING If there is a light leak in the imaging chamber based on a test using the High Reflectance Hemisphere there is also a strong possibility that X rays may be leaking from the chamber For this reason do not use the IVIS Spectrum CT in CT mode until a radia
70. m CT Components Cleaning the Anesthesia Manifolds and Stages NOTE Do not allow fluids to get into the gas port and especially the exhaust port of the stage Do not allow fluids to get into the interior of the manifold It is recommended that you cover the ports with masking tape before cleaning or disinfecting the components If fluid does enter the passages try blowing it out ei Figure 7 10 Dual Mouse Manifold Gas and Exhaust Ports There are two gas ports and four exhaust ports Gas port Gas port Exhaust ports To clean the anesthesia manifold and stage 1 Wipe the components with a cloth dampened with warm detergent 2 Wipe the components with a cloth dampened with water 3 To disinfect the manifold spray with a 5 solution of bleach and distilled water Do not allow fluids to enter the interior manifold passages y NOTE Beds and light baffles are not considered cleanable and should be replaced periodically 7 10 X ray Door and Panel Interlocks A safety interlock prevents unintentional opening of the imaging chamber door for more details see Chapter 5 page 21 A solenoid interlock serves two functions it 1 acts as a switch which completes the electronic logic so that image acquisition can begin and 2 mechanically locks the door during imaging so that it cannot be accidently opened These safety interlocks protect the user from X ray exposure during CT imaging and protect the
71. ms Technology Support For more details on fluorescent imaging see page 45 Figure 7 6 Filter Wheel 33 IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 7 8 Specimen Warming System The imaging platform is temperature controlled to keep subjects warm during imaging The temperature control is enabled after the instrument is powered on and initialized from the Living Image software The default temperature is 37 C and is self monitoring after the system is initialized The actual surface temperature of the padded rotating animal stage may be lower than that of the imaging platform The imaging platform does not have active cooling The platform may require up to 20 minutes to passively cool from 37 C to ambient temperature 7 9 Rotating Stage and Anesthesia Connections Table 7 1 lists the types of anesthesia connections manifolds that are available Each manifold is equipped with its own gas and exhaust tubes The ends of the tubes have gendered fittings so that the gas and exhaust tubes cannot be connected incorrectly IMPORTANT Disconnect the manifolds at the gas block so that the gas tube is not inadvertently connected to the exhaust port Animal death is the likely outcome if the mouse nose cone is connected to the vacuum exhaust Table 7 1 Anesthesia Manifolds Type Description For Use With These Imaging Modalities Single Mouse CT Manifold One anesthe
72. n Protection Programs amp Release Prevention Element Dept of Environmental Protection P O Box 415 Trenton NJ 08625 0415 609 984 5636 http www state nj us dep rpp index htm NEW MEXICO Radiologic Technologist Cert Program New Mexico Environment Department 1190 St Francis Drive Santa Fe NM 87502 0110 505 476 3264 http www nmenv state nm us nmrcb radserv html NEW YORK Office of Radiologic Health 2 Lafayette Street 11th Floor New York NY 10007 212 676 1550 http www health state ny us environmental radiological radon registration htm NORTH CAROLINA North Carolina Radiation Protection Section 3825 Barrett Drive Raleigh NC 27609 7221 919 571 4141 Ext 232 http www ncradiation net NORTH DAKOTA Division of Air Quality North Dakota Dept of Health 918 E Divide Avenue Bismarck ND 58501 1947 701 328 5188 www ndhealth gov ag rad OHIO Bureau of Radiation Protection Ohio Dept of Health 246 North High Street Columbus OH 43215 614 644 2727 http www odh ohio gov odhPrograms rp radprot radprot1 aspx OKLAHOMA Consumer Protection Services State Department of Health 1000 Northeast Tenth Street Oklahoma City OK 73117 1299 405 271 5243 74 IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 75 Table 11 2 US Radiation Authorities continued OREGON Radiation Protection Services Oregon Health Services
73. n automatic background accumulation and self diagnostics overnight The platform temperature and the computer monitor may be turned off If it is necessary to shut down the system for any reason such as long term storage more than 30 days it is important to follow the procedure below 1 2 3 Close the Living Image software and save any information of interest at the prompt Turn off the computer Turn off any gas to the imaging chamber and disconnect the gas supply if necessary If you are using the XGI 8 Gas Anesthesia system follow the shutdown procedure in the XG 8 Anesthesia System User Manual Turn off the main circuit breaker switch located on the rear of the IVIS Spectrum CT and disconnect the power cord from the wall socket Cover the gas inlet and outlet ports with masking tape to prevent entry of dust 6 Remove any loose objects from the imaging chamber and close the door If you plan to move the system see important information on page 65 If you have any problems during the shutdown procedure please contact IVIS technical support 8 9 Emergency System Shutdown Procedure In the event of an emergency where the IVIS Spectrum CT must be shut off immediately push in the red mushroom shaped Emergency Stop switch located on the main console control panel Figure 8 6 Figure 8 6 Emergency Stop button EMERGENCY STOP To enable the system for restarting turn the switch in a clockwise direct
74. ncluding the camera and motor driven components Push in the X RAY ARMED button When the imaging chamber door is shut X rays can be generated from the IVIS Acquisition Control Panel within the Living Image software on the acquisition computer D NOTE For bioluminescent or fluorescent imaging only the Emergency Stop switch must be in the READY out position 42 IVIS Spectrum CT Hardware Manual Chapter 8 Operating the IVIS Spectrum CT 43 8 2 Restarting the System After a Power Outage The same procedure is used to restart the system after an intentional shutdown or shutdown due to a power outage 1 Confirm that the rear panel switch is off and the power cord is plugged into the console and the wall socket 2 Turn on the main power switch on the rear panel 3 Turn on the computer and monitor Start the Living Image software In the Living Image software click Initialize TVIS System in the control panel Figure 8 2 The status light is red Figure 8 2 Control panel in the Living Image software Field of View cm Subject height 1 50 Focus use subject height v Temperature MS 5 Wait until the camera has cooled to operating temperature requires about 10 minutes You can monitor the system temperature in the Living Image software The status light is green when the system is ready for operation 8 3 Gas Plumbing A WARNING Use only
75. nd start the Living Image software 7 Initialize the system click Initialize in the control panel within the Living Image software IVIS Spectrum CT Hardware Manual Chapter 9 Troubleshooting 53 The status light is red and turns green when initialization is complete Wait until the camera has cooled to the demand temperature before acquiring images Pattern Illuminator The pattern illuminator is not user serviceable If you notice any changes to the alignment grid for example change in the brightness or position of the grid contact Caliper technical support Lamp Replacement The quartz halogen lamp that provides the fluorescent light source typically operates for thousands of hours depending on the lamp level low or high This light source is housed in a compartment that contains hazardous voltages and is not user accessible or serviceable ZA VOLTAGE DO NOT attempt to service the quartz halogen lamp If the lamp brightness is decreased or if lamp replacement is required contact Caliper technical support 9 7 Accessing the Area Under the Imaging Platform During normal operation the imaging platform always returns to the loading position before the door can be opened This helps ensure user safety and convenience when loading subjects Occasionally you may need access to the area beneath the platform for cleaning or to retrieve a subject Since the platform moves rapidly and produces strong forces the area beneath
76. ning and safety devices incorporated in the IVIS Spectrum CT Point out the importance of having them in working condition Proper operating procedures for the equipment a If the trainee will be conducting radiation surveys discuss the operation calibration and limitations of radiation survey instruments a Proper survey techniques if surveys are conducted by non Caliper personnel Methods of controlling radiation dose such as time distance and shielding Principles and practice of maintaining X ray exposure to as Low As Reasonably Achievable ALARA Personal monitoring Symptoms of acute localized exposure and proper reporting procedure for an actual or suspected exposure a Applicable state provincial local and institutional regulation or policies 6 Specifications Electrical Power Requirements CCD Camera Optical Imaging Optics on page 26 X ray Source on page 26 X ray Detector on page 26 Fluorescent Imaging Components on page 27 Environmental Temperature and Humidity on page 27 Weight and Dimensions on page 27 6 1 Electrical Power Requirements Table 6 1 Electrical power requirements Requirement Specification Nominal voltage required main console 100 120VAC 50 60Hz 12A or 220 240VAC 50 60Hz 6A Overvoltage Category Il for 2500V max transient Maximum operation voltages 90V to 264V 10 Recommended dedicated circuit 20 A for 120VAC or 10 Amps minimum for 230VAC For more det
77. nts or replacing the optical filters A clear window at the top of the imaging chamber protects the lenses and other optical components from contamination Do not touch or allow subjects to touch this window otherwise image quality may be impaired Further the glass window could be cracked or broken if struck by a hard object The lens protection window should be cleaned periodically To clean the surface of the lens protection window 1 Blow off any accumulated dust using a Dust Off compressed gas duster 2 Dampen a lint free cloth with isopropyl alcohol and gently swab the window surface to remove any smudges or streaks 3 Repeat Step 2 using a clean area of the cloth or a new cloth until the window is clean 4 Take an image to confirm that the image quality is restored 10 4 Changing the Emission Filters for Fluorescence Imaging This section explains how to change one or more of the optional emission filters used for fluorescence imaging in the IVIS Spectrum CT D NOTE After changing a filter or a filter position you must also change the corresponding filter label in the Living Image software For more details see the Living Image Software Manual for the IVIS Spectrum CT part no 134222 1 Before you begin gather the following tools a 5 32 hex key 2 Phillips head screw driver One pair of lint free powderless gloves Figure 10 1 Tools required for deactivating the solenoid lock 5 32 Hex driver
78. of distilled water WARNING When preparing the ethanol solution adhere to the 25 by volume pure ethanol concentration A higher alcohol concentration may be flammable and a lower concentration may not suppress organic growth in the cooling system or camera Paper towels or cloth for clean up a The coolant fill bottle supplied with the IVIS Spectrum CT Coolant Replenishment Procedure VOLTAGE Do not remove any panels from the console until you turn off the IVIS Imaging System and disconnect the power cord Only trained personnel should perform the following procedures Serious risk of electric shock is possible if instructions are not followed completely 1 Turn off electric power to the main console a Turn off the main power switch on the rear panel and disconnect the console power cord from both the wall socket and the imaging console b Move any cables away from the lowest rear panel of the console so that the panel can be removed Figure 10 3 No other cables need to be removed IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance 63 Figure 10 3 Lower rear panel power cord removed 2 Remove the console rear panel a Use a 3 32 hex head wrench to remove the screws that secure the bottom rear fan panel Figure 10 2 b Carefully remove the panel and lean it against the side of the console CAUTION Be careful not to pull too hard on the rear fan panel
79. otographic Image is Unacceptable Default camera controls are programmed during the initialization of the Living Image software Changes to these settings can greatly affect the photographic image Confirm that the f stop and binning levels are set to the default conditions If you have questions about the photographic settings please contact Caliper technical support Refer to the Living Image Software Manual for the IVIS Spectrum CT part no 134222 for further details on acquiring images IVIS Spectrum CT Hardware Manual Table 9 1 Troubleshooting photographic images Chapter 9 Troubleshooting Problem Possible Cause Corrective Action Image is Subject moved during Check to see if the subject may have moved If the subject is streaked the exposure not on the sample stage it is probably on the floor of the imaging chamber If the sample has moved locate and re anesthetize it If gas anesthesia is being used confirm that the anesthesia is turned on and the flow rate is appropriate Image is blurry Subject height is not correctly entered in the Living Image software If you are using the Sample Height setting to determine the focus confirm that the correct height of the sample is entered If you are using the Manual setting to determine the focus confirm that the focus has been manually adjusted The f stop for photographs should be set to f 8 An f stop smaller than 8 reduces the depth of fie
80. ph radiation htm 71 IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Ordering Information 72 Table 11 2 US Radiation Authorities continued LOUISIANA Emergency amp Radiologic Services Division PO Box 4312 Baton Rouge LA 70821 225 219 3041 www deq louisiana gov portal tabid 2283 Default aspx MAINE Division of Environmental Health Radiation Control Program 286 Water Street 4th Floor Augusta ME 04333 Telephone 207 287 5677 http www maine gov dhhs eng rad MARYLAND Radiologic Health Program Maryland Dept of the Environment 1800 Washington Blvd Suite 750 Baltimore MD 21230 1724 410 537 3300 http www mde maryland gov Programs AirPrograms Radiological Health MASSACHUSETTS Radiation Control Program Department of Public Health Schrafft Center Suite 1M2A 529 Main Street Charlestown MA 02129 617 242 3035 www mass gov dph rcp MICHIGAN Radiation Safety Section Div of Health Facilities 8 Services Bureau of Health Systems MI Dept of Community Health PO Box 30664 Lansing MI 48909 517 241 1993 www michigan gov rss MINNESOTA Section of Indoor Environments amp Radiation Division of Environmental Health Department of Health 625 Robert Street N P O Box 64975 St Paul MN 55164 0975 651 201 4602 http www health state mn us divs eh radiation xray IVIS Spectrum CT Hardware Manual Chapter 11 Contact and Or
81. produce X rays when the CT function has been armed and energized The instrument may also be operated in standard bioluminescent or fluorescent mode without X ray generation The IVIS Spectrum CT is defined by most regulatory agencies as a Cabinet X Ray System A cabinet system is one that produces little or no X ray exposure to the user and is safe to operate with the user in close proximity Caliper certifies that the IVIS Spectrum CT produces not more than 0 5 millirem per hour at a distance of 5 cm from the instrument surface The instrument is also certified to meet all international exposure requirements typically 0 1 millirem per hour and other regulations that apply to the user s location The IVIS Spectrum CT meets all US FDA regulations regarding a cabinet X ray system For information on international limits for X ray doses see page 18 Product Documentation Users will receive pre installation information to help prepare the laboratory for installation of the IVIS Spectrum CT and any accessories purchased with it Table 5 1 lists the documents provided in PDF format on CD with the instrument Table 5 1 Documents provided with the IVIS Spectrum CT Imaging System Document Name Description Part No Safe Operating and Emergency Explains safe operating and emergency procedures for 133919 Procedures for the Operation of the the IVIS Spectrum CT Provides a list of contacts for Spectrum CT Cabinet X ray System radiation prot
82. quisition Control Panel and select an appropriate excitation and emission filter Figure 8 4 Figure 8 4 Control panel in the Living Image software Field of View Service 13 5 cm Subject height 1 50 an Focus use subject height y Temperature Locked Luminescent imaging mode Fluorescent imaging mode Fluorescent Imaging A 150 Watt quartz tungsten halogen lamp with a dichroic reflector provides the fluorescence excitation light The Living Image software controls the lamp intensity level The fluorescence light source is a module located behind the rear panel and is not user serviceable The lamp output is delivered to the excitation filter wheel assembly located in the rear compartment where it is collimated and transmitted through a fluorescence excitation filter The 12 excitation filter wheel locations provide 10 fluorescence filters One filter slot contains a light block that is used IVIS Spectrum CT Hardware Manual Chapter 8 Operating the IVIS Spectrum CT 46 during bioluminescent imaging to prevent external light from entering the imaging chamber The 12 position excitation filter wheel is motorized and controlled through the Living Image software Upon leaving the excitation filter the light enters an optical switch that directs the light to either the top of the imaging chamber epi illumination or to the bottom of the stage transillumination When epi illumination is selected
83. s Patent Applications 09 905668 Multi view Imaging Systems 10 606976 Method and Apparatus for 3 D Reconstruction of Light Emitting Sources 10 151463 Method and Apparatus for Determining Target Depth Brightness and Size Within a Body Region 10 189886 Fluorescence illumination assembly for an imaging apparatus and 10 068573 Light calibration device for use in low level light imaging systems 4 3 Trademarks IVIS and Living Image are registered trademarks or trademarks of Caliper Life Sciences Inc The names of companies and products mentioned herein may be the trademarks of their respective owners Apple Macintosh and QuickTime are registered trademarks of Apple Computer Inc Microsoft PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries Adobe and Illustrator are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and or other countries Intel is a registered trademark of Intel Corporation 5 X ray Safety and Radiation Hazards Introduction Radiation Theory and X ray Generation on page 16 Biological Effects of Radiation on page 17 IVIS Spectrum CT Safety Systems on page 19 Regulatory Compliance and Laboratory X ray Safety Procedures on page 23 5 1 Introduction The IVIS Spectrum CT produces X ray radiation This radiation is confined to the interior of the imaging chamber Spectrum CT will only
84. s in the Field of View on page 44 Optical Imaging Basics on page 45 CT Imaging Basics on page 47 Normal System Shut Down Procedure on page 48 Emergency System Shutdown Procedure on page 48 The IVIS Spectrum CT is intended for use in bioluminescent fluorescent and CT imaging procedures The system is designed to detect extremely low level light emissions that are orders of magnitude dimmer than can be detected by the naked eye The IVIS Spectrum CT allows you to monitor and record cellular and genetic activity within a living organism in real time The imaging system captures quantifies and images the light emitted by a sample Additionally the computed tomography CT modality allows structural imaging of the subject 8 1 Starting the IVIS Spectrum CT All components of the IVIS Spectrum CT should be left on unless the system will not be used for more than 30 days It is also important to leave the system on to enable automatic overnight electronic background measurements Periodically rebooting the computer is permissible and does not affect camera operation Figure 8 1and Table 8 1 show the main console controls and indicators Figure 8 1 Main console controls and indicators Status light Key switch for the X ray generator in OFF position Primary X ray indicator X ray armed switch and indicator Emergency OFF switch IVIS Spectrum CT Hardware Manual Table 8 1 Status lights and indica
85. s the door is completely closed An actuator key is inserted into the solenoid activated ON OFF switch when the instrument door is closed This completes the electrical circuit for the X ray tube After the X ray circuit is turned ON the actuator key 1s captured by a solenoid lock which prevents the door from opening while X rays are being generated A secondary redundant door interlock prevents X ray generation when the instrument door is opened Figure 5 2 Primary and secondary safety interlock switches on the imaging chamber door Secondary interlock Primary interlock switch and solenoid activated lock 21 IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards Figure 5 3 shows the primary solenoid lock with its activation key engaged and disengaged to demonstrate its principal of operation When the solenoid is activated by electrical power the key is retained by a mechanical lock The solenoid lock also acts as the primary switch which completes the circuit that allows imaging to begin Figure 5 3 Primary door interlock and activation key EG Solenoid lock Activation key Activation key engaged in the solenoid lock A second redundant door interlock prevents X ray generation by physically removing part of the X ray electrical circuit when the imaging chamber door is opened Figure 5 4 demonstrates the principal of operation for the redundant interlock A copp
86. sia nose cone is built Luminescent into a stage which is placed on the imaging platform Fluorescent with epi illumination Stage Fluorescent with Bed reduces X ray transillumination scattering Dual Mouse CT Manifold Luminescent Fluorescent with epi illumination Two anesthesia nose cones are Bed reduces X Light baffle prevents ouilt into a stage which is placed ray scattering light from one mouse n the imaging platform shining on the other 34 IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components Table 7 1 Anesthesia Manifolds continued Type Description For Use With These Imaging Modalities Five Mouse Luminescent Manifold Fluorescent with epi illumination Animals are placed directly onto the imaging platform when using the Five Mouse Manifold The Spectrum CT also accommodates the Mouse Imaging Shuttle MIS Caliper part no 134366 The MIS can be used in CT imaging as well as luminescent or fluorescent epi illumination and transillumination imaging However degradation of CT images can be expected due to the plastic walls of the MIS CAUTION Do not use the Mouse Imaging Shuttle with FOV A The height of the shuttle could break the glass window at the top of the imaging chamber Single Mouse Manifold The Single Mouse Manifold can be used with all imaging modalities The manifold is integrated with a stage containing a low density compo
87. sible light and X rays with wavelengths ranging from 400 to 10 nanometers Non visible ultraviolet light is also considered an ionizing radiation but it does not possess the penetrating capability of X rays In summary X ray radiation is an ionizing form of electromagnetic radiation that has sufficient energy to break chemical bonds The IVIS Spectrum CT uses an X ray generating tube to produce ionizing radiation which if left un shielded could be harmful to people or animals The IVIS Spectrum CT instrument includes shields that confine the X rays within the imaging chamber Consequently operation of the IVIS Spectrum CT does not expose the operator to unsafe X ray exposure At least two complete radiation surveys are conducted to verify the instrument design ensure proper manufacturing and installation and make certain that there is no leakage from the instrument that exceeds the US and international regulations X rays lonizing Radiation As noted above X rays are classified as ionizing radiation that is capable of removing or rearranging the electronic bonds of chemical compounds For this reason they are considered potentially hazardous to living organisms X rays can also interact with matter by scattering off of atoms in new directions Therefore the IVIS Spectrum CT incorporates shielding that completely blocks scattered radiation in all possible directions including the primary beam direction Ionizing radiation can also be pro
88. site foam and carbon fiber bed that reduces X ray scatter during CT imaging The bed has 171 holes which enable trans illuminated fluorescence imaging The bed is a delicate part and should be handled carefully Figure 7 7 shows how to install the bed into the animal stage Figure 7 7 Installing the Bed in the Single Mouse Manifold Stage Leaf Stage Gently push the left edge of the bed against the edge of the stage cutout then snap the bed down into place The plastic leaf springs on the left side of the cutout will secure the bed 35 IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 36 Figure 7 8 Removing the Bed from the Single Mouse Manifold Stage m Gently lift up at the corners to remove the bed Gas Connections The distal ends of the gas tubes from the animal stage have gendered Luer fittings which prevent incorrectly connecting the tubes to the GAS and EXHAUST connections on the imaging platform Figure 7 9 IMPORTANT Disconnect the GAS and EXHAUST connections at the outlet port not at the animal bed IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectrum CT Components 37 Figure 7 9 Gas and Exhaust Luer Fittings Do not disconnect the tubes here Connect the distal ends of the tubing to the mated GAS and EXHAUST fittings on the imaging platform IVIS Spectrum CT Hardware Manual Chapter 7 IVIS Spectru
89. t A VOLTAGE A voltage warning indicates high voltage or risk of electric shock Observance of safety precautions will help avoid actions that could damage or adversely affect the performance of the IVIS Spectrum CT If the equipment is used in a manner not specified in this manual the protection provided by the equipment may be impaired IVIS Spectrum CT Hardware Manual Chapter 2 Important Safety Instructions 2 2 Instrument Labels Pay careful attention to the labels on the instrument Table 2 1 shows the safety symbols that are found on the IVIS Spectrum CT or in this manual Table 2 1 Instrument labels Label Description Where located on the IVIS Spectrum CT the pinch hazard label indicates the location of a potential pinch point Pinch hazard Where located on the IVIS Spectrum CT indicates a high voltage hazard High voltage Where located on the IVIS Spectrum CT indicates an X ray radiation hazard P X ray radiation hazard Where located on an IVIS Spectrum CT operating in Canada indicates an X ray radiation hazard lt X ray radiation hazard Canada Where located on the IVIS Spectrum CT indicates that important information about the labeled area can be found in the IVIS Spectrum CT Hardware Manual Read manual 2 3 Instructions A WARNING The IVIS Spectrum CT should only be operated by personnel who have been trained in radiation s
90. t so that you do not damage the connectors or fan grills After the system is positioned at the new location lock the casters Reconnect the cables and power cord If necessary reinstall the dome Restart the system Confirm that the rear panel power switch is off and all cables are reconnected Confirm that the power cord is plugged into the instrument and the wall socket Turn on the rear panel main power switch Turn on the computer and start the Living Image software o 29 pp Initialize the system from within the Living Image software The status light is red The status light turns green when the system is initialized Wait until the camera has cooled to the demand temperature before you begin an imaging session about 10 minutes 11 Contact and Ordering Information PerkinElmer Contact Information Ordering Information US Radiation Authorities on page 69 Canadian Radiation Authorities on page 78 11 1 PerkinElmer Contact Information Technical Support Telephone 1 877 522 2447 US 1 508 435 9500 E mail tech support caliperls com Fax 1 508 435 3439 Mail Caliper Life Sciences US Corporate Headquarters 68 Elm Street Hopkinton MA 01748 Caliper Sales To order replacement parts contact Caliper at Telephone 1 508 497 3302 E mail order entry caliperls com Fax 1 508 435 0967 Mail Sales Order Management Caliper Life Sciences 68 Elm Street Hopkinton MA 01748 USA 11 2 Ordering Information Ta
91. t to allow the door to be fully opened and provide the recommended air space behind the instrument An additional area that is 48 inches 121 9 cm wide should be available preferably to the right of the instrument to accommodate a table or cart to hold the computer and provide a work surface 2 7 Power Considerations Power Sources The IVIS Spectrum CT is configured for the voltage requirements of the installation locality that was specified at the time of order The set voltage is marked on the outside of the product itself and should only be powered by that voltage If the system is moved to another area check to make sure that the same voltage requirements exist at the new location An IVIS Spectrum CT that operates on 120VAC requires a dedicated 20 Amp circuit that is not shared with other loads except the computer and the optional XGI 8 Gas Anesthesia System Ensure that all equipment is plugged into a properly grounded AC supply The computer should share the same circuit to avoid ground loops Never use auxiliary power outlets on other equipment to supply the IVIS Spectrum CT Since the system contains internal surge protection it must be powered directly from the main electrical supply wall socket Use the surge protector provided by Caliper for the computer and other accessories An IVIS Spectrum CT configured for 230 VAC requires a dedicated minimum 10 Amp circuit subject to the same conditions described above For more details
92. ter gene such as luciferase It allows noninvasive visualization and monitoring of cellular and genetic activity within a living organism in real time For fluorescence imaging the instrument can operate in reflectance or transillumination mode Filtered light from a broad band lamp provides the excitation source in both modes In the reflectance mode light is delivered to four reflectors that are located on the ceiling of the imaging chamber In IVIS Spectrum CT Hardware Manual Chapter 1 Welcome the transillumination mode the excitation light is delivered to an x y translation assembly under the stage and focused to a 2 mm diameter beam that can be directed to a particular location on the underside of the animal subject The system includes 10 excitation and 18 emission filters that enable spectral scanning of reporters over the range from 480 850 nm X ray CT Imaging The IVIS Spectrum CT includes a high speed micro CT system for small animal X ray imaging It uses an X ray source and a flat panel X ray detector to produce high resolution 3D images of mouse skeletal structure and surrounding soft tissue The acquisition computer incorporates a fast reconstruction method for high throughput workflows 3D Tomography The IVIS Spectrum CT imaging system uses CT images to reconstruct the surface topography The concentration and 3D anatomical location of fluorescent sources can be computed from CT images and transillumination fluorescent
93. tic camera dome when you move the IVIS Spectrum CT though a doorway or other low overhang To remove the dome wrap a flat blade screw driver with a thin cloth or paper towel and use the screw driver to gently pry the dome off at the rear of the instrument IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance 66 Moving the System If the instrument is only repositioned a short distance within the range of the power cord and cables the power need not be turned off However be careful not to accidentally turn off the power when gripping the system at the right rear When possible two people should move the IVIS Spectrum CT The instrument is tall and it is difficult to see potential obstacles when pushing the instrument from behind Make sure that the path is clear of items that could jolt the casters and cause the instrument to tip Move the instrument slowly so that you are always in control of its motion To move the system 1 mom Turn off the main power switch on the rear panel of the instrument and disconnect the power cord from the wall socket and the instrument Remove all cables from the rear panel of the instrument CAUTION Any cables that dangle from the instrument could cause the system to topple if caught by a caster Unlock the four casters Grip the instrument at a convenient location but do not use the door handle as a grip Be careful when gripping the right rear of the instrumen
94. tics almost all tape plants paint rodent food mostly plants mouse urine and animal bedding have been found to glow Use caution when introducing materials into the IVIS Spectrum CT It is advisable to prescreen all items by imaging them alone before imaging them with samples under study Caliper recommends using non powdered gloves when working with IVIS Spectrum CT equipment 8 7 CT Imaging Basics Computed Tomography imaging is an X ray based imaging modality that is used to generate a 3D process During image acquisition projection images planar X ray images are acquired from a series of perspectives For the Spectrum CT the orientation for each projection represents the angle of the animal with respect to the center line from the X ray source at the back of instrument to the X ray detector at the front of the instrument This stack of project images is then reconstructed to generate a 3D image of the physical object The image reconstruction process assumes that the animal does not move during acquisition As a result the motion caused by breathing and heartbeat causes inherent degradation of the image resolution which is seen as blur The X ray based imaging process can also cause artifacts X rays also attenuate in a variety of materials in proportion to the linear attenuation coefficient and the amount of material in the X ray path The linear attenuation coefficient u is related to the atomic number Z and is often norma
95. tion survey has been completed 9 4 No Optical Image is Produced If no optical image is produced there may be an error in the Living Image software a problem with the physical connections to the camera or a hardware failure To troubleshoot the problem 1 Close the Living Image software and restart the computer 2 Restart the Living Image software and try to acquire an image If no image was produced after restarting the computer contact Caliper technical support IVIS Spectrum CT Hardware Manual Chapter 9 Troubleshooting 52 9 5 No CT Image is Produced Verify that the X rays were armed Verify that nothing has been left on the shelf that could block X rays for example the 5 port manifold then perform the steps listed above in No Optical Image is Produced 9 6 Hardware Problems Most of the components in the IVIS Spectrum CT are not user serviceable due to the integrated design of the instrument VOLTAGE Do not remove any panel from the IVIS Spectrum CT except for the filter wheel access panel or the lower rear cart panel Removing a panel could expose the user to hazardous voltages The procedure for removing the filter wheel access panel is described on page 60 Removing the lower rear cart panel for access to the chiller is described on page 62 If you suspect a problem with the instrument hardware contact Caliper technical support Circuit Breaker Power Entry Module Under normal operation
96. to perform the test Contact Caliper technical support for information regarding these tests or for scheduling a Caliper trained technician to conduct the survey as part of an overall safety check 10 2 Maintenance and Safety Checks Daily Safety Checks The following safety checks should be performed on a daily basis 1 Verify that the door interlocks discussed in Safety Interlocks on page 21 are in good repair 2 Verify that the key switch functions properly 3 Verify that the following X ray ON indicators are functioning properly when the X ray modality is used These include the Red light on the front control panel Red light under the translucent dome at the top of the instrument a X ray ON indicator in the IVIS Acquisition Control Panel in the Living Image software 4 Verify that the amber X ray armed indicator light is working 5 Light leak check see page External light contamination page 51 IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance 58 Weekly Safety Checks The following safety checks should be performed on a weekly basis 1 All of the daily safety checks 2 Inspect the solenoid lock and the redundant interlock assembly and make sure that it has not loosened Inspect the screws holding the two mating keys 3 Inspect the metal knife edges on the door and the light box for damage such as bending The knife edges keep X rays inside the light box and prevent light from entering
97. tors Chapter 8 Operating the IVIS Spectrum CT Item Location Description Front panel of the console Indicates the general status of the IVIS Spectrum CT Green The imaging system is ready Red The imaging system is not ready Front panel of the console X RAY key switch A key actuated control which ensures that X rays can only be generated when the key is in the ON I position Front panel of the X RAY ON console Primary X ray indicator A red warning light that is illuminated when X rays are being generated Front panel of the console X RAY ARMED button This button must be pushed in to enable X ray generation Under the top dome of the console X ray ON indicator light A red light which illuminates when X rays are being generated and is visible from 360 degrees IVIS Acquisition Control Panel in the Living Image software This icon appears in the IVIS Acquisition Control Panel when X rays are being generated To start the IVIS Spectrum CT 1 2 3 Turn on the instrument main power switch on the rear of the console Turn the key switch on the front console to the ON position Confirm that the EMERGENCY STOP switch is in the READY out position If necessary turn the knob clockwise to reset it to the READY out position D NOTE Pushing in the Emergency Stop switch cuts off power to the entire instrument so all functions stop i
98. tube described on page 17 Some cabinet parts incorporate additional shielding to prevent leakage There are no ports apertures or other openings through which any part of the human body can be placed when X rays are being generated A WARNING Do not modify or remove any of the steel shielding in the IVIS Spectrum CT If the instrument door does not completely close do not operate the IVIS Spectrum CT and contact Caliper technical support The IVIS Spectrum CT Control Panel The IVIS Spectrum CT is controlled primarily by software through a proprietary Caliper program Some control functions are located on the main console electronics panel in compliance with requirements for cabinet X ray systems However the controls and indicators on the main module are enablers only The initiation and termination of an X ray imaging session is controlled from the computer through the Spectrum CT software Figure 1 1 shows the IVIS Spectrum CT and its main console electronics panel X rays can only be generated when the main console is armed for X ray mode This means that the Emergency Stop switch is in the OUT position the key switch is in the ON position and all safety interlocks are working X rays can be terminated from the computer as the result of ordinary operation at the programmed end of image acquisition or from the computer control panel X ray generation can be aborted by pushing the Emergency Stop button IN or by turning OFF the ke
99. uccessor During the Limited Warranty Caliper will repair or replace at Caliper s sole option any defective parts if such repair or replacement is needed because of System malfunction or failure to conform to published specifications during normal usage in accordance with the instructions in this manual Repairs and replacements under the Limited Warranty will be made at Caliper s expense Caliper s limit of liability under the Limited Warranty shall be the purchase price of the Imaging System Caliper shall not be liable for any other losses or damages These remedies are the Customer s exclusive remedies for breach of this Limited Warranty No coverage or benefits shall be provided under this Limited Warranty if any of the following conditions apply a The System has been subjected to unauthorized modifications e g unauthorized installation of hardware or software unauthorized repair or servicing misuse neglect abuse accident alteration any use inconsistent with or in contradiction to the instructions in this manual or other acts which are not the fault of Caliper b Caliper was not advised in writing by the Customer of the alleged defect or malfunction of the System within the earlier to occur of ten 10 days after the expiration of the Limited Warranty period or 15 days after becoming aware of the defect or malfunction c If Customer moves the System from its installed location to another location without Caliper
100. uter system for data acquisition and analysis Living Image system software IVIS Spectrum CT Hardware Manual Chapter 1 Welcome Figure 1 1 IVIS Spectrum CT Imaging System and optional accessories CCD eds XGI 8 Gas Anesthesia System optional High resolution monitor Acquisition Compartment with computer the thermoelectric chiller unit and camera power supply XWS 260 Workstation optional The sound limiting compartment beneath the imaging chamber contains the thermoelectric cooling unit camera power supply and other imaging chamber functions All components except the computer are integrated into a single moveable chassis The IVIS Spectrum CT imaging capabilities include Bioluminescent imaging a Fluorescent imaging reflectance and transmission modes a Low dose ultra fast micro CT for small animals Together the IVIS Spectrum CT and Living Image system software enable 3D optical tomography for fluorescence and bioluminescence 3D volumetric tomography of CT images Automatic co registration of 3D reconstructions of luminescent or fluorescent sources optical image data with CT images 3D volumetric data Multi spectral fluorescence and spectral unmixing a Cerenkov imaging for optical radiotracer imaging Optical Imaging The ultra sensitive CCD camera of the IVIS Spectrum CT enables bioluminescent imaging which detects the light emitted by living cells expressing a repor
101. ved or replaced with filters with other spectral characteristics For information on how to replace a filter see Changing the Emission Filters for Fluorescence Imaging page 60 The excitation filters are housed in a filter wheel module that is not accessible to the user Unlike the emission filters the user cannot replace these filters Contact Caliper technical support if an excitation filter must be replaced Black Paper Although the platform is black anodized it is recommended that you image items on a high quality black paper especially biological specimens Caliper has surveyed many types of paper and recommends Swarthmore Artagain Black part no 445 109 size 8 5 inch x 11 inch Caliper part no 117837 This paper prevents illumination reflections and helps keep the stage clean It can be cut with scissors to make non luminescent background of custom shapes Low Fluorescence Mat When operating the system in the fluorescent mode use the Low Fluorescence Imaging Mat part no 119000 set of 10 to reduce background fluorescence The mat is made from a low reflectance textured plastic that is easily cleaned The plastic mat can be cut with scissors to make non fluorescing background of custom shapes IVIS Spectrum CT Hardware Manual Chapter 8 Operating the IVIS Spectrum CT 47 Glowing Materials Always keep in mind that nearly EVERYTHING glows that is has the potential to phosphoresce and contaminate the image Most plas
102. y of the compound for the organism of interest Consider dedicating an imaging system for immunodeficient animals This will reduce the risk of cross contamination ll Cleaning the Imaging Platform It is recommended that you use a lint free wipe such as Scott Pure wipe or a Kaydry EX L wipe to minimize the presence of particulate matter in the imaging chamber After saturating a lint free wipe clean the internal surfaces using a gentle circular motion Do not pour or spray the solution directly onto internal surfaces Rinse surfaces using a wipe saturated with sterile deionized water Do not allow puddles of water to remain on the surfaces Avoid cleaning the ceiling ofthe image chamber except when necessary When cleaning the chamber ceiling use extreme care to avoid damaging the lens protection window and the light diffusers in this area To avoid any phosphorescence from the cleaner make sure that the surfaces are dry before using the imaging chamber Cleaning the Rotating Stage Itis particularly important not to allow any liquids to drip into the rotating stage assembly The stage is mounted on a precision bearing that can become corroded over time if it is exposed to water or other cleaning liquids IVIS Spectrum CT Hardware Manual Chapter 10 Care and Maintenance 60 Cleaning the Lens Protection Window Please contact Caliper technical support for information about cleaning or sterilizing any of the optical compone
103. y switch y NOTE The Emergency Stop button will turn off power to the entire system A redundant interlock as prescribed by the United States FDA completely disconnects the power to the X ray source if the door is opened to a gap of 5 mm 0 2 inches The X ray leakage seal of the door incorporates an overlap edge design which prevents radiation escape through this gap Also during X ray operation a solenoid operated door lock prevents opening of the door This door lock remains engaged for four seconds after X ray power has been turned off This ensures that the door IVIS Spectrum CT Hardware Manual Chapter 5 X ray Safety and Radiation Hazards 20 can only open after the X ray source voltage is turned off and the radiation field has completely collapsed Requirements for Turning on X rays X rays can be generated when the following conditions are met The Emergency Stop button is in the ON out position Figure 5 1 The keyed on off switch is in the ON position This keyed switch is the main ON OFF enabling switch on the IVIS Spectrum CT console control panel B The X ray Armed push button is on and lighted The instrument door is closed and locked engages the primary and redundant safety interlocks The filter wheel access panel is attached oa pw X rays are activated from the Living Image software Figure 5 1 IVIS Spectrum CT console control panel Status light Key switch for the X
104. you will be required to register the IVIS Spectrum CT with the state or provincial radiation protection authority This registration may require periodic renewal Study Documentation Before operating the IVIS Spectrum CT you should read and understand this manual as well as the software manuals provided with the instrument Pay particular attention to the safety procedures described in Safe Operating and Emergency Procedures for the operation of the Spectrum CT Cabinet X ray System PN 133919 This document can help you prepare a radiation safety plan which is described in the next section Create a Radiation Safety Plan Y our institution may require that you have a written radiation safety plan Such plan may already exist at your institution and may also be a requirement of your registration with the state or provincial radiation protection authority Here are some key points of a radiation safety plan Persons using the IVIS Spectrum CT must read all documentation supplied with the instrument Permit only trained and authorized individuals to operate the Spectrum CT Wear personal radiation monitors if required Caliper recommends wearing them even if they are not required Designate a master key person who controls access to the Spectrum CT If required install the instrument in a controlled access or restricted access room Post any required Caution X ray signs required by your regulating authority Designate a person responsible for
105. ystem see Restarting the System After a Power Outage page 43 Overloading WARNING Do not overload wall outlets extension cords or integral convenience receptacles as this can result in a risk of fire or electric shock For electrical power requirements see page 25 2 8 Cleaning or Moving the System Components Cleaning Liquid Entry VOLTAGE Do not use liquid or aerosol cleaners and never spill liquid of any kind on any of the IVIS Spectrum CT components Sprays and liquids that come into contact with the IVIS Spectrum CT may result in damage to the system or electrocution For more details on proper care of the system see Cleaning the IV S Spectrum CT page 58 Moving the IVIS Spectrum CT The IVIS Spectrum CT is a sensitive scientific instrument and should be moved with care Pay particular attention when rolling the instrument on its casters to avoid toppling the equipment The total height of the instrument is 77 inches 195 6 cm If necessary the plastic dome can be removed for easier passage through doorways For more details on removing the dome see page 65 If you have any questions about moving the instrument contact Caliper technical support 2 9 Other Equipment or Chemicals The use of any equipment other than that recommended by this manual has not been evaluated for safety and therefore is the sole responsibility of the user No chemicals are required for the operation of the IVIS Spectrum CT

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