tacoTM Instrument user manual
Contents
1. e Press Yes to confirm and return to the self testing screen or pressing No to continue the reaction POCKIT 1 0 0101 01 01 01 01 01 2011 14 4 5 Switch Off e After the reaction switch off the main power and unplug the SD card to read the raw data on a PC or Mac POCKIT 1 0 0101 01 01 01 01 01 2011 Reaction Completed 1 2 8 4 5 6 7 8 50m 06006006 0 550m 0 000 Buzzer OFF Main power Caution Ensure the reaction has been completed before shutting down the device 15 Section 5 Cleaning and Maintenance 5 1 General Care Use only clean and wet paper towel or cotton to wipe out stains in the reaction chamber Do not disassemble the POCKIT for cleaning as it will void the warranty Do not use solvent detergent or bleach to clean the POCKIT surface Note Always wear gloves and always switch off and unplug the POCKIT before cleaning 5 2 Dealing with Spills e Wipe out visible spills with disposable paper towel immediately and contact your local distributor or GeneReach directly for further assistance e Do not use UV light to disinfect POCKIT as it will cause device damage 16 Troubleshooting a b c d e f g h 9 Observation or Problems Self test runs more than 5 minutes System crash SD card reading error No display on screen System error Temperature error Camera error Time sequencing error Create data d2. POCKIT 1 0 0101 01 01 01 01 01 2011 Reaction Completed l 2724 86 7 8 s20onm 0 0060000 50m OQ 000 Temperature Warning Buzzer OFF Caution The variation of ambient temperature cannot be more than 5 C during the reaction 12 4 5 Display the Result e If a single detection wavelength either 520 nm or 550 nm is chosen in the beginning the screen will display the result as the examples shown below POCKIT 1 0 0101 01 01 01 01 01 2011 POCKIT 1 0 0101 01 01 01 01 01 2011 Reaction Completed Reaction Completed 123 4 56 7 8 128486 7 86 50mm OQ OQ 0000 som OQ 0007 9 Buzzer OFF Buzzer OFF e If both detection wavelengths 520 nm 550 nm are chosen in the beginning the screen will display the result as the example shown below POCKIT 1 0 0101 01 01 01 01 01 2011 Reaction Completed 1 2 3 4 5 6 7 8 520m Q 7 00972 9 5501mm O Buzzer OFF e Description of the results e nagie NY Undetermined Note Please interpret the results accordine to the user manual of the kits used 13 4 6 Review the Raw Data Insert the SD card on a PC or Mac Find the folder of the reaction and a file named raw csv e Please refer to the kit manual for data interpretation 4 7 Stop during the Reaction e Press Abort to stop the reaction POCKIT 1 0 0101 01 01 01 01 01 2011 Remaining 52 min CH 520 550 nm Folder 201101 20110101 010101 Abort
3. Create Data Directory Error please check SD card and press OK 4 4 Run iiPCR Reaction Place the holder containing R tubes into the reaction well Close the lid and press Run to start the reaction POCKIT 1 0 0101 01 01 01 01 01 2011 System Ready CH 520 550 nm Note Ensure the holder containing R tubes fit the reaction wells completely Caution Do not look into the LED light directly from reaction wells The screen will display the remaining reaction time and the folder name of the reaction POCKIT 1 0 0101 01 01 01 01 01 2011 Remaining 52 min CH 520 550 nm Folder 201101 20110101 010101 Caution Should any of the circumstance below occurs the result will be unreliable e The lid was opened or the SD card was removed during the POCKIT reaction e The ambient temperature of the reaction was not in the range of 15 35 C e The variation of ambient temperature was greater than 5 C during the reaction Il e When the reaction is complete there will be beep sound from POCKIT Press Buzzer OFF to turn off the sound POCKIT 1 0 0101 01 01 01 01 01 2011 Reaction Completed 1 2 8 44 65 6 7 8 s20onm 0608060606 0 50m 00000 d Buzzer OFF i nz Note If there is no beep sound after the completion of the reaction please see Troubleshooting NS e If the reaction temperature is abnormal during the reaction it will show Temperature Warning on the screen
4. Serial number Alternative current Consult instruction before use Risk of personal injury to the operator or a safety Caution hazard to the surrounding area This product is required to comply with the WEEE symbol European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC Section 1 Introduction The POCKIT Nucleic Acid Analyzer POCKIT is designed for performing insulated isothermal polymerase chain reaction 1PCR It provides 11PCR based analysis and is equipped with two optical channels 520 nm 550 nm for multiplex detection The qualitative test results are readily displayed on the LCD monitor and automatically saved in a SD card It allows a single user to load amplify and interpret up to 8 nucleic acid samples within 1 hour 1 1 Intended Use POCKIT is intended to provide qualitative detection of nucleic acid targets using fluorescence based 1PCR reagents It is intended for in vitro use only and for research purposes Section 2 General Information 2 1 2 2 2 3 2 4 Product Components e POCKIT Nucleic Acid Analyzer e Power Cord e 1 User Manual e Warranty Card e 1 Holder Specifications Dimensions W x D x H 280 mm x 250 mm x 85 mm Net Weight 2 1 kg Power Supply 100 120 200 240 V AC 50 60 Hz 2A Operating Temperature 15 35 C Testing Capacity Number of samples per run 1 8 Reaction volume 45 55 ul per reaction Equipments
5. JLIEKI I nace sc anayzer USER MANUAL GeneReach Biotechnology Corp TEL 886 4 24639869 FAX 886 4 24638255 No 19 Keyuan 2nd Road Central Taiwan Science Park Taichung City Taiwan 407 www genereach com 2011 11 CONTENT Sy MDO DES TONA A WARE 1 SEEGON Introductio akad inicio ita Edi 2 BAZA 2 Section Zs GEneraLInioEmal Ona O ROA OOOO 3 Zell Product C OMPONCINS wia WE cook i odd 3 Ded Oe Cl CAMO E R P APANC Seace dani E E O 3 Zo SC AOAC Ny cette EO A acne de T T 3 2 4 Equipments and Materials Required But Not Provided ccccccccccssssssseeceeeeeeeeeeeeseeeeeeeeeeaas 3 2SIEIONENIC M enh haben sata sa ad AE OC da e otoki ada BEE 4 ZORIENTOWANA AA ON E eee ee 5 DECHON O IMDOFCANC NOS a din da NSRS 6 ssb a DOMA 1 a8 8 KOC PN ce OO O AA JE 6 SECUOMA OCP GPA COM A O OWA ORAS 7 BLOWIENOR R nii OG eet raced i od aS cna 4 2 Choose Detection Wavelength zac w co ALE OE ROL a a Ah 7 0SEL1Up Dateand TIMO wacka EEEE E 9 AA TRUM IPC Re IRE ACIO Ms aa sc RO ck GG noc ec 11 0 AIS PAY US RESUME cu tracert O EO OZONE 13 AO Review the Raw Dalia aaa kk ER AEO ORT OOP AE 14 A StOp dunne NO REACION wadia OE ania din dE 14 ROD WICKO AOR EPEE AE 15 Section 5 Cleaning and Mamicha Cee ass GO Gi Od s d Giwi 16 Sy ROM OT A CS GE woda OE Od dok da nen eae 16 522 Dean Wilh Pits ea lidia ci AGE 16 TEOUDICSMOO CMG A W Z Ri O az wa zadac GEE WoW LEI 17 Symbols gt gt o AEG Date of manufacturing Manufacturer Catalogue number
6. and Materials Required But Not Provided e Nucleic acid extraction kit e Nucleic acid amplification reagents and kits e 200 ul and 1000 ul tips and 1 5 ml tubes e R tubes and Caps cubee mini centrifuge e Disposable gloves Note To avoid power failure connecting POCKIT to an uninterrupted power system UPS and or voltage regulator is highly recommended 2 5 Front View n ZAZ RRZZJ ELLE P i ia Isolates the heat source and prevents light 1 interference on the reaction chamber Connects the R tube holder to perform the 2 Reaction well o amplification Cooling vents Releases the heat generated during reaction A touchscreen where users input the operation 4 Control panel commands and view the results A slot where users insert the SD card to operate 5 SD card slot POCKIT and save reaction raw data c6 Holder Loads R tubes into the reaction wells 2 6 Rear View i ml m fl m U m in iu a nin 5 NJ Section 3 Important Notes Check the device upon receiving If any items are missing or harmed please contact your local distributor or GeneReach immediately for replacement Do not use any damage items as they may lead to poor performance of the test or harm the user 3 1 Safety Information DO NOT OPEN LID DURING REACTION e Do not touch any plug and electric switch with wet hands e Make sure to keep the device away from water e Switch off and unplug the device befor
7. e moving it e Pack the device carefully before moving and delivering Vibration or crash may cause damage e Do not look into the LED light directly from reaction wells e Only use consumables and accessories provided from GeneReach e Make sure the ambient temperature during reaction is kept at 15 35 C e SD card should not be removed until the device is switched off e Hold the plug while pulling it out from the electrical outlet Do not pull the power cord directly e Do not place the power cord on any subject with high temperature e User should revalidate the device if it has been moved e Do not disassemble the device if any problem occurs Contact your local distributor or GeneReach directly for troubleshooting Section 4 Operation 4 1 Switch On e Plug in the POCKIT and switch on the main power located in the back The device will complete self testing within 5 minutes POCKIT 1 0 0101 01 01 01 01 01 2011 ZIICKIT soso Note If self testing runs more than 5 minutes please see Troubleshooting 4 2 Choose Detection Wavelength e After the self testing choose the detection wavelength according to the kits or methods used and press OR POCKIT 1 0 0101 01 01 01 01 01 2011 O 520nm O 550nm e 520nm 550 nm SRR Lok D Se System Ready will be shown after choosing the detection wavelength POCKIT 1 0 0101 01 01 01 01 01 2011 System Ready CH 520 550 nm Befor
8. e the reaction starts if the user wants to change the detection wavelength press CH Select and choose again POCKIT 1 0 0101 01 01 01 01 01 2011 System Ready CH 520 550 nm Time seting CF Select 4 3 Set up Date and Time e Press Time Setting POCKIT 1 0 0101 01 01 01 01 01 2011 System Ready CH 3520 550 nm a a Tine Seng e Touch the numbers that needs to be changed on the screen Press Up and Down to select the desired number Press OK to confirm and return to the System Ready screen Press Cancel to quit and leave the Time Setting screen POCKIT 1 0 0101 01 01 01 01 01 2011 2011 Year 01 Month 01 Day 01 Hour 01 Min 01 Sec Note The data derived from each test is stored in the SD card and in a folder named according to the corrected if applicable date and time when the user switches on the analyzer If any Errors listed below are shown please see Troubleshooting POCKIT 1 0 0101 01 01 01 01 01 2011 Temperature Error please check the environmental temperature POCKIT 1 0 0101 01 01 01 01 01 2011 Camera Error please call for service POCKIT 1 0 0101 01 01 01 01 01 2011 Times Sequencing Error please call for service POCKIT 1 0 0101 01 01 01 01 01 2011 SD Card Error please check SD card and press OK POCKIT 1 0 0101 01 01 01 01 01 2011 System Error please call for service POCKIT 1 0 0101 01 01 01 01 01 2011
9. irectory error No beep sound Comments and Suggestions Re start the device Contact your local distributors or GeneReach for assistance Discard current batch of R tube with reagents Re start the device prepare new reaction reagents and R tube and repeat the reaction If any further problems occur please contact your local distributors of GeneReach for assistance Insert SD card turn off POCKIT and restart Contact your local distributors or GeneReach for assistance 1f the problem persists Check the plug to see 1f it has been connected to the power system Check the fuse to see 1f it is overloaded Contact your local distributor or GeneReach for assistance Contact your local distributors or GeneReach for assistance Check the ambient temperature to be kept at 15 35 C Contact your local distributors or GeneReach for assistance Contact your local distributors or GeneReach for assistance Contact your local distributors or GeneReach for assistance Check storage space of SD card Check the file folder number is within 512 in each directory Contact your local distributors or GeneReach for assistance Contact your local distributors or GeneReach for assistance 17
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