Model Pico Plus Syringe Pump Manual
Contents
1. 13 52 0 2254 0 0135 0 0002 0 0017 26 28 0 4379 0 0262 0 0004 0 0041 66 17 0 0001 1 103 0 0661 0 0011 0 0081 131 7 0 0002 2 196 0 1317 0 0021 0 0162 264 7 0 0003 4 412 0 2647 0 0044 0 0402 656 7 0 0007 10 94 0 6567 0 0109 0 0806 1317 0 0014 21 94 0 0001 1 317 0 0219 0 1612 2637 0 0027 43 94 0 0002 2 637 0 0439 0 4018 6580 0 0067 109 7 0 0005 6 580 0 1097 0 5689 9313 0 0095 155 2 0 0006 9 313 0 1552 0 8056 9999 0 0135 219 6 0 0009 13 16 0 0001 0 2196 1 610 0 0269 439 4 0 0017 26 36 0 0002 0 4394 1 Harvard Apparatus Pico Plus Syringe Pump Appendix D Serial Communication Serial connector RJ 11 style pinning Position Signal 1 GND 2 GND 3 TxD 4 RxD The connector position as viewed looking into the RJ 11 connector reading left to right Baud rate and address The baud rate and address are set in the CONFIG mode options are described as follows a Pump Address To set the pump address at the RATE VOL CONFIG display move the underline cursor using the W or A key to CONFIG Press the SET key to enter the CON FIG mode options Use the W or A to scroll the CONFIG options until you reach the SET ADDRESS Press the SET key again moving the underline cursor to the right Use the W or A to scroll the two digit number fields Use the W or key and the SET key to display a unique pump address from 00 to 99 Press the ENTER key to select the d2. to 99 1 Harvard Apparatus Pico Plus Syringe Pump Appendix E Dual RS 232 RXD 4 TXD GND GND IN Host Computer Connection GND GND TXD RXD OUT Chain Connection to Additional Pump s Harvard Apparatus pumps are distributed on a non exclusive basis by INSTECH Instech Laboratories Inc Headquarters 5209 Militia Hill Road Plymouth Meeting PA 19462 TL 800 443 4227 TL 610 941 0132 FX 610 941 0134 European sales office P O Box 2 9950 AA Winsum The Netherlands TL 0031 0 594 696123 www instechlabs com Pumps syringe
3. Syringe Pump Operation Advanced Features 3 Display Intensity For varying light conditions four levels of intensity can be set on the vacuum fluorescent display To set the desired display intensity at the RATE VOL CONFIG display move the underline cursor using the or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET INTENSITY Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the SET INTENSITY choices 1 2 3 4 4 is highest intensity 1 is the lowest intensity Press the ENTER key to select the desired display intensity Exit the CONFIG mode by pressing the ENTER key again and save the INTENSITY setting in memory Changing Rates If the pump is running at an existing rate it will continue to do so until a new rate is entered Except for volume mode the flow rate can be changed while the pump is running As soon as the ENTER key is pressed the pump will change to the new flow rate To change rates from the keypad while in vol ume mode the pump must be stopped first The pump must be stopped to change the flow rate range units while in either mode Reversing the Flow Direction The pump is equipped with a reversing switch to toggle from infuse to with draw The flow rate will be the same for both infuse and withdraw modes unless changed manually refer to 4 CAUTION Rem
4. are microliters per hour Set flow rate and range units are milliliters per hour Set target infusion or refill volume units are set in the flow rate range choosen Numbers can be between 0 and 1999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded E Harvard Apparatus Pico Plus Syringe Pump Appendix D Serial Communication Queries DIA Returns diameter value units in mm RAT Returns rate value set in current range units VOL Returns current accumulated infused volume units in ml or pl depending on range VER Returns model and version number of firmware TAR Returns target volume units in ml or pl depending on range value format nnnn nnn The returned value is an 8 character string with leading zeros converted to SP characters ACSII 32 decimal The fifth character is a decimal point ASCII 46 decimal Queries with string response RNG Returns range message character string either ML HR ML MN UL HR UL MN NL HR NL MN PL HR and PL MN Error responses CR LF CR LF prompt Unrecognized command CR LF OOR CR LF prompt Entered value in out of range Remote Communication Set up The pico plus is equipped at the factory for remote communications The communication format is Baud Rate User settable 300 1200 2400 9600 Parity None Stop Bits 2 Data Bits 8 Duplex Half Address User settable 00 default
5. pressed the underline cursor below the digit or character on the display moves one step to the right It is used in conjunction with the ascending and descend ing keys When it the display shows the desired the correct value the set key will advance right to the next digit AV The ascending and descending keys are used to change the numbers on the display Up key makes numbers increase V Down Key makes numbers decrease When the underline cursor is placed below the decimal point the AV keys shift the decimal one place up or down Run Indicator When the pump is running the highly visible green LED above the RUN STOP key will illuminate 2 Harvard Apparatus Pico Plus Syringe Pump Operation Getting Started 3 Enter Syringe Size Enter the inside diameter ID of the syringe you wish to use Units are in mil limeters mm If you do not know your syringe diameter refer to appendix B for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use Press SET followed by the DIAM key The previously used diameter will appear on the display The underline cursor will appear under the left most digit or decimal point The and V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right O
6. Harvard Apparatus Pico Plus Syringe Pump General Information Serial Number All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibration All syringe pumps are designed and manufactured to meet their performance spec ifications at all rated voltages and frequencies A calibration certificate is available upon request Contact customer service for details and pricing Warranty Harvard Apparatus warranties this instrument for a period of two year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact your local distributor or Harvard Apparatus Inc 84 October
7. Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 valid only in the U S outside U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair U T l parts When ordering please describe parts as RESEARCH USE ONLY completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service NOT FOR CLINICAL USE ON PATIENTS CAUTION This pump is not registered with the FDA and is not for clinical use on human or veterinary patients It is intended for research use only 5 Harvard Apparatus Pico Plus Syringe Pump General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manua
8. Label Toggle Switch Power Cord Receptacle 2 Harvard Apparatus Pico Plus Syringe Pump Installation Initial Set up Read the manual to become familiar with all features and functions of the pico plus Connect the external DC power supply and line cord to the pump and main supply Turn on main power switch located on the rear panel The display will now illuminate indicating that the power connections are correct The display will indicate POWER FAIL this is normal as the pump indicates on the display if power was disrupted since last use Location Requirements for the Syringe Pump A sturdy level clean and dry surface Minimum of one inch 2cm clearance around the pump Appropriate environmental conditions A well ventilated room Loading the Syringe 1 10 11 Press the bronze halfnut button 1 on the side of the pusher block to release it from the leadscrew While holding the button in slide the pusher block 2 to the left Loosen plunger retaining bracket 3 and syringe retaining bracket 4 by slightly unscrewing the cap screws Loosen the thumbscrew on the syringe clamps 5 and rotate the clamp out of the way Lay the syringe into the V shaped slots on the syringe holder block 6 Swing the syringe clamps 5 over the syringes and tighten the thumbscrews to secure the syringe in place Slide the syringe retaining bracket 4 up to secure the flange on the syr
9. Model Pico Plus Syringe Pump User s Manual 70 2213 2 Harvard Apparatus Pico Plus Syringe Pump Table of Contents SUBJECT PAGE NO Table of G ntents save fedes 1 General Information Warranty Repairs 1 2 2 General Safety Summary ica 3 4 Intro dUCION aser od ee rore retira liana 5 Theory of Operation rere titre cott 5 Featulfes rat 5 Front and Rear Views iei teet naaa 6 Installation diia 7 Initial Setup amp Location Requirments 2 7 Loading the Syringe ie 7 Operation agli 8 Getting Started unica eiae IR NE EENE 8 Turn Pump ON peno pen eee 8 Function Keys and Run Indicator ess 8 Entering Syringe Size iore 9 Entering Flow Rate Range ciare aa 9 Entering Flow inside 9 Press Run Footswitch iii 9 Check Syria 9 Advanced Features reete aste ines 10 Volume Mode int lalla 10 Power Pall titer toed radiali 10 Changing Rates ati or cede pe UO AER 11 h Eiusdem 11 Protecting Small Fragile Syringes ss 11 Appendices Asp SPECIICATIONS use 12 B Table of Popular Syringe Diameters eee 13 C Table of Minimum and Maximum Flow Rates 14 D Serial Communication eese 16 19 E RS232 Connectionsas lalla eco 20
10. arget volume Press the RUN key each time you want to repeat the volume dispense Ifyou press the STOP key during a volume dispense you can restart the pump at the place you stopped by pressing the RUN key again To exit the volume dispense mode set the target volume to 00 00 or turn off and on the pump via the main power switch NOTE In the event of a power failure the actual dispensed volume and the target volume are not retained in memory This means that while in volume dispense mode if a power failure occurred the pump would not resume volume dispense pumping even if the POWER ON mode was set to run Power Failure In the event of a momentary or prolonged power failure the pico plus can be set to either a Resume pumping when power is returned with POWER FAIL on the display b Not start pumping when power is returned with POWER FAIL on the display To set the power fail mode at the RATE VOL CONFIG display move the underline cursor using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET POWER ON display Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the POWER ON choices stop or run Press the ENTER key to select either mode Exit the CONFIG mode by pressing the ENTER key again and save the POWER ON setting in memory 1 Harvard Apparatus Pico Plus
11. e all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION 1 Functional Ground Refer to Manual Terminal Enviromental Conditions Indoor use only Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to 80 RH non condensing Well Ventilated Room Altitude up to 2000 m Mains Voltage Fluctuation not to Exceed 10 of Nominal Transient Over Voltage Category II Pump is Rated Pollution Degree 2 in Accordance with IEC 664 E Harvard Apparatus Pico Plus Syringe Pump Introduction Theory of Operation The pico plus is designed as a low cost infusion pump capable of delivering very low flow rates at low to moderate back pressures The pump can hold two syringes of any make from 0 5 pl to 10ml The diameter of the syringes is entered via the keypad and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow Non volatile memory stores the last syringe diameter and flow rate along with other configuration data The Power Failure Mode can be set to either turn the pump off in the event of power failure or to resume pumping when power resumes Features Bright Display and Easy To Use Interface A new two line 16 character display along with six membrane keys make this a most attractive but powerful easy to use syringe pump Only two entries are required to start pumping syringe Inside Diameter mm and pumping flow rates The Flow rate can be changed w
12. ember to check the switch before pressing run to ensure it is indicating the desired direction Maintenance Keep the pump clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw and guide rods It is important to keep these guide rods clean and lubri cated To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the display window For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alcohol If the pump does not work properly contact Harvard Apparatus for appropri ate instructions Protecting Small Fragile Syringes The pico plus will hold microliter size syringes down to 0 5pl size These small syringes have fine wire plungers that may be damaged if allowed to bot tom out Be sure to set the limit switches properly to prevent damage to small syringes Harvard Apparatus Pico Plus Syringe Pump Appendix A Pico Plus VPF Specifications Type Syringe Size for Pico Plus with Holder For 2 Syringes Flow Rate Range Minimum Maximum Calibration Display Nonvolatile Memory Maximum Force Maximum Pressure Drive Motor Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Range Leakage to Ground Ground Resistance Input Power Input Power Connection Power Suppl
13. esired pump address Exit the CONFIG mode by press ing the ENTER key again and save the ADDRESS setting in memory b Baud Rate To set the pump address at the RATE VOL CONFIG display move the underline cursor using the W or A key to CONFIG Press the SET key to enter the CON FIG mode options Use the W or A key to scroll the CONFIG options until you reach the SET BAUD Press the SET key again moving the underline cursor to the right Use the W or A key to scroll the SET BAUD choices 300 1200 2400 or 9600 Press the ENTER key to select the desired baud rate exit the CONFIG mode by pressing the ENTER key again and save the BAUD setting in memory 4 Harvard Apparatus Pico Plus Syringe Pump Appendix D Serial Communication Operation with Serial control and external Run Stop switch foot switch Mode Function Local Keyboard Run Stop Remote Serial control Run Stop Exit Remote Operation Momentary switch contact If pressed when stopped starts pump motor Run LED lights when running If pressed when running stops pump motor REMOTE mode initiates when pump receives a CR character ASCII 13 REMOTE is displayed on pump display while in remote mode RUN Commands the pump motor to start STP Commands the pump motor to stop KEY Commands the pump to exit REMOTE mode Keyboard Run Stop key while REMOTE mode will stop pump motor if running Will not restart pump motor in REMOTE mode While
14. hile the Pump is running Direct enter flowrates in ml pl nl and pl Two Modes of Operation Constant Flow Rate and Volume Dispense The pico plus will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode All modes can be operated from key pad footswitch included or external controller via serial connection included Withdraw mode makes it easy to load capillaries pipettes and syringes Adjustable end of travel limit stop to protect small syringes Smooth Flow Enhanced micro stepping pump profiles deliver very smooth and consistent flow that is virtually pulse free Nonvolatile Memory The pump remembers its last syringe size flow rate used and configuration settings Power Fail Mode In a power failure the Pump can either RESUME or STOP pumping when power is returned CE Mark Approved UL CSA ETL The pico plus meets all relevant European EMC and Safety requirements for lab oratory equipment 2 Harvard Apparatus Pico Plus Syringe Pump Pico Front and Rear Views Syringe Clamp 5 Syringe Holder 6 Sa Allen Wrench Plunger Retaining Bracket 3 Pusher Block 2 4 2 A Adjustable Stop e EPG b Run LED Collar 8 Syringe Retaining Bracket 4 A UN Adjustable Cover Limit Switch sep Leadscrew CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS Power Switch Foot Pedal Reversing Receptacle Serial
15. in REMOTE mode External Run Stop connection is ignored 2 Harvard Apparatus Pico Plus Syringe Pump Appendix D Serial Communication Commands Queries and Responses After each transmission to the pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR The prompt character indicates the status of the pump as follows prompt V detection LF prompt meaning ASCII code When stopped ASCII 58 decimal When running forward ASCII 62 decimal When stalled ASCII 42 decimal Pump 11 does not include stall Serial commands and their meanings Commands KEY RUN STP CLV CET REV Return to keyboard control Exit remote mode Start infuse forward direction Stop motor Clears volume accumulator to zero Clears target volume to zero dispense disabled Start Reverse Direction Commands with numbers MMD PLM NLM number number number number number number number number number number Set syringe diameter units are mm Rate is set to zero after MMD command Set flow rate and range units are Picoliters per minute Set flow rate and range units are Nanoliters per minute Set flow rate and range units are microliters per minute Set flow rate and range units are milliliters per minute Set flow rate and range units are Picoliters per hour Set flow rate and range units are Nanoliters per hour Set flow rate and range units
16. inge barrel against the syringe holder block Press the halfnut button 1 and hold it in while sliding the pusher block up to the syringe plunger Tighten the thumbscrews on the plunger retaining bracket 3 The infuse limit switch is adjusted by loosening the thumbscrew on the back of the pusher block and sliding the stop pin 7 in or out to the desired loca tion to protect the syringe from bottoming out The withdraw limit switch enclosed in the cover is adjusted by securing the stop collar 8 into the desired position 2 Harvard Apparatus Pico Plus Syringe Pump Operation Getting Started Getting started 1 N Turn Pump ON Turn on power using the switch on rear of the pump the display will light and indicate POWER FAIL this is normal as the pump indicates on the dis play if power was disrupted since last use Function Keys and Run Indicator Refer to the colored keypad at the front of the pump to identify the following functions starting from the right Hees RUN STOP This turns the pump motor on and off also accomplished by pressing the foot switch ENTER This key enters the data that is on the display into the memory of the pump Also used to query the flow rate DIAM Used to enter or query the syringe diameter SET This key is used to select which digit of the display is to be changed to move the decimal point and to move between modes Each time the set key is
17. l If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Power Supply The pump is supplied with an approved power supply and line cord To maintain the safety integrity of the device use only one of the following power supplies Ault Inc Model No PWI118 Output 12 Vdc 1 5A Input 100 250 Vac 50 60 Hz 5A Cui Stack Model No SA06N12 V Ouptut 12 Vdc 2 0 A Input 100 240 Vac 50 60 Hz 8A Globtek Inc Model No GT 4201D 12 Output 12 Vdc 1 66 A Input 100 240 Vac 50 60 Hz 0 6A Use Proper Line Cord Use only the line cord shipped with the product and make sure line cord is certified for country of use Ground the Product This product is grounded through the return path of the DC power supply To avoid electric shock use only approved power supply and line cord with the product Make Proper Connections Make sure all connections are made properly and securely Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections E Harvard Apparatus Pico Plus Syringe Pump General Safety Summary Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Observ
18. nce the desired diameter is displayed press the ENTER key to place this value into memory Enter Flow Rate Range Choose your flow rate units picoliters nanoliters microliters or milliliters per minute or per hour From the initial RATE VOL CONFIG menu using the A or V key move the underline cursor under the CONFIG mode menu prompt Press the SET key to enter the CONFIG mode Press the SET key again to move the under line cursor to the flow rate choices Choose your flow rate units while in the SET UNITS mode by pressing the A or V keys to scroll the four flow rate choices ml min ml hr pl hr Once the desired flow rate units dis played press the ENTER key to return to the main SET CONFIG mode Press SET or ENTER again to put your desired flow rate units into memory and return to the RATE VOL CONFIG menu Enter Flow Rate From the initial RATE VOL CONFIG menu press the SET key to enter the SET RATE mode Each time you change the syringe diameter the previously used flow rate is erased If the syringe diameter is unchanged the previously used flow rate will appear on the display The underline cursor will appear under the left most digit or decimal point The A and V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired rate is displayed press the ENTER key to place this value into memory Press RUN Or Foot Switch Press the RUN STOP ke
19. r Tite All Plastic 10 14 57 6 12 70 Size Diameter 12 15 90 2 5 CC 2 mm Hamilton Microliter a 1 7 Series Gastight Popper amp Sons Inc Size Diameter Perfektum Glass _ ei _ 5 ul 0 103 Size Diameter Unimetrics mm 0 25 3 45 MM Series 4000 amp 5000 1 0 1457 0 5 3 45 Size Diameter 2 0206 1 4 50 5 0 3257 a 25 0 729 25 0 729 da 1358 100 1 460 108 148 250 2 300 250 23 500 3 260 500 3 26 1000 4 610 1 0ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 Harvard Apparatus Pico Plus Syringe Pump Appendix C Flow Rates Syringe 10 mm Min 0 1 77 78 9999 1 296 9999 0 0778 1241 0 0013 20 69 ful 0 15 172 2 2 870 0 1722 2794 0 0029 46 57 2ul 0 21 338 9 5 648 0 3389 5477 0 0056 91 28 Sul 0 33 827 8 13 80 0 8278 9999 0 0138 225 4 10ul 0 46 1606 26 76 1 606 0 0268 437 9 25ul 0 73 4044 67 41 4 044 0 0674 1103 50ul 1 03 8043 134 1 8 043 0 1341 2196 100ul 1 46 269 4 16 16 0 2694 4412 250ul 2 3 668 4 40 11 0 6684 9999 1000ul 3 26 1344 80 61 1 344 im 4 61 2686 161 2 2 686 2 5ml 7 28 6696 401 8 6 697 3ml 8 66 9481 568 9 9 482 5ml 10 3 805 6 13 43 10 14 57 1610 26 83 A table of minimum and maximum diameter values pL hr max diameter 1 14 pL mn max diameter 8 89 mL mn min diameter 0 22 Appendix C Flow Rates ul min ml hr ml min min max min max 0 0001 1 241 0 0011 0 0003 2 794 0 0027 0 0004 5 477 0 0054 0 0009
20. y Size HxWxD Weight Microprocessor dual syringe infuse withdraw Plastic or glass From 0 5 ul to 10 ml 1 3 pl min with 0 5 pl syringe 0 4393 ml min with 10cc syringe Automatic enter syringe size up to 16 mm Inside Diameter 2 line 16 character vacuum fluorescent display and green run led Stores diameter rate and configuration settings 25 Ib 100 p s i with 10cc syringe 175 p s i with 1 ml syringe 1 8 step angle geared 36 1 motor 1 pulse in 27 6 sec 200 steps sec 0 0444 um pusher advance per motor step 0 0388 y m min 0 8333 mm min 1 to 16 384 Typically lt 150 pA Typically lt 0 05 ohms 12 VDC 1 5Amps 2 5mm ID x 5 5mm OD male plug 100 250 VAC 50 60 Hz 18 Watts External Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 11 4 x 22 9 x 11 4 cm 4 1 2 x 9 x 4 1 2 in 2 3 kg 5 Ib Actual force is higher but not recommended for applications requiring more than 25lbs of force Appendix B Syringe Inside Diameter 13 am DS Terumo Stainless Steel SGE Size Diameter Size Diameter Scientific Glass 3cc 8 95 mm 8cc 9 525 mm Engineering 5 13 00 Size Diameter 10 15 80 Becton Dickinson 25 ul 0 73 mm 1 cc 4 78 mm 50 1 03 Plastic Plasticpak 100 1 46 Diameter _ 1 cc 4 78 mm BO ui Sherwood Monoject 3 8 66 Plastic 5 12 06 1 0ml 4 61 mm Size Diameter 10 14 50 2 5 7 28 1 cc 4 65 mm __ 10 30 3 8 94 Ai
21. y or press the foot switch to start pump and begin pumping The Run Indicator Green LED above the RUN STOP key will light when the pump is on and pumping Check Syringe Often The pico plus pump will shut itself off when the syringe is empty via the infuse limit switch If the switch is improperly set or the syringe is overloaded the pump will not shut itself off Although this presents no hazard to the user or the pump it is prudent to check the syringe from time to time 1 Harvard Apparatus Pico Plus Syringe Pump Operation Advanced Features 1 Volume Mode The pico plus can be set to dispense a precise volume and then stop To acti vate the volume dispense mode a target volume must be set To set a target volume at the RATE VOL CONFIG display move the under line cursor using the or V key to VOL Press the SET key to enter the VOL set mode Use the A or V key and the SET key to display a target volume from 00 01 to 99 99 Volume units are pl picoliters nl nanoliters ml mil liliters or pl microliters Target volume units are established in the CONFIG SET UNITS mode example if your pumping units are ml min or ml hr then the volume dispense units will be ml s Press the ENTER key to select the desired target volume Exit the VOL mode by pressing the ENTER key Once you press the RUN key the pump will run until the target volume is delivered The display will show the actual volume dispensed along with the t
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