Model 11 VPF Syringe Pump User`s Manual
Contents
1. is used in conjunction with the ascending and descend ing keys When it the display shows the desired the correct value the set key will advance right to the next digit AV The ascending and descending keys are used to change the numbers on the display A Up key makes numbers increase V Down Key makes numbers decrease When the underline cursor is placed below the decimal point the AV keys shift the decimal one place up or down Run Indicator When the pump is running the highly visible green LED above the RUN STOP key will illuminate Harvard Apparatus Pump 11 VPF Syringe Pump Operation Getting Started 3 5 Enter Syringe Size Enter the inside diameter ID of the syringe you wish to use Units are in mil limeters mm If you do not know your syringe diameter refer to appendix B for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use Press SET followed by the DIAM key The previously used diameter will appear on the display The underline cursor will appear under the left most digit or decimal point The A and V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired diameter is displayed press the ENTER key to place this value into memory Enter F2. 0 or turn off and on the pump via the main power switch NOTE In the event of a power failure the actual dispensed volume and the target volume are not retained in memory This means that while in volume dispense mode if a power failure occurred the pump would not resume volume dispense pumping even if the POWER ON mode was set to run Power Failure In the event of a momentary or prolonged power failure the Pump 11 VPF can be set to either a Resume pumping when power is returned with POWER FAIL on the display b Not start pumping when power is returned with POWER FAIL on the display To set the power fail mode at the RATE VOL CONFIG display move the underline cursor using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET POWER ON display Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the POWER ON choices stop or run Press the ENTER key to select either mode Exit the CONFIG mode by pressing the ENTER key again and save the POWER ON setting in memory J Harvard Apparatus Pump 11 VPF Syringe Pump Operation Advanced Features 3 Display Intensity For varying light conditions four levels of intensity can be set on the vacuum fluorescent display To set the desired display intensity at the RATE VOL CONFIG display move the underline cursor
3. 3 mm min 1 to 16 384 Typically lt 150 pA Typically lt 0 05 ohms 12 VDC 1 5Amps 2 5mm ID x 5 5mm OD male plug 100 250 VAC 50 60 Hz 18 Watts Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 11 4 x 22 9 x 11 4 cm 4 1 2 x 9 x 4 1 2 in 2 3 kg 5 Ib Actual force is higher but not recommended for applications requiring more than 25lbs of force Harvard Apparatus Pump 11 VPF Syringe Pump Appendix B Syringe Inside Diameter Terumo Size Diameter 3 cc 8 95 mm 5 13 00 10 15 80 1 ec 4 78 mm Sherwood Monoject Plastic Size Diameter 1 cc 4 65 mm 3 8 94 6 12 70 12 15 90 Popper amp Sons Inc Perfektum Glass Size Diameter 0 25 cc 3 45 mm 0 5 3 45 1 4 50 2 8 92 3 8 99 5 11 70 10 14 70 Stainless Steel Diameter 9 525 mm Size 8 cc Becton Dickinson Plastic Plasticpak Size Diameter 1 cc 4 78 mm 3 8 66 5 12 06 10 14 50 Air Tite All Plastic Size Diameter 2 5cc 9 60 mm 5 0 12 45 10 15 90 Unimetrics Series 4000 amp 5000 Size Diameter 10 ul 0 460 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size Diameter 5 pl 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1 0
4. Model 11 VPF Syringe Pump User s Manual Model 11 Virtualy Pulse Free Dual Syringe Series MAI 55 2111 HARVARD APPARATUS WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply wit
5. Syringe Often The Pump 11 VPF will not shut itself off when the syringe is empty or is oth erwise overloaded Although this presents no hazard to the user or the pump it is prudent to check the syringe from time to time 5 Harvard Apparatus Pump 11 VPF Syringe Pump Operation Advanced Features L 2 Volume Mode The Pump 11 VPF can be set to dispense a precise volume and then stop To activate the volume dispense mode a target volume must be set To set a target volume at the RATE VOL CONFIG display move the under line cursor using the A or V key to VOL Press the SET key to enter the VOL set mode Use the A or V key and the SET key to display a target vol ume from 00 01 to 99 99 Volume units are either ml milliliters or ul microliters Target volume units are established in the CONFIG SET UNITS mode example if your pumping units are ml min or ml hr then the volume dispense units will be ml s Press the ENTER key to select the desired target volume Exit the VOL mode by pressing the ENTER key Once you press the RUN key the pump will run until the target volume is delivered The display will show the actual volume dispensed along with the target volume Press the RUN key each time you want to repeat the volume dispense If you press the STOP key during a volume dispense you can restart the pump at the place you stopped by pressing the RUN key again To exit the volume dispense mode set the target volume to 00 0
6. equencies A calibration certificate is available upon request Contact customer service for details and pricing Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact your local distributor or Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 valid only in the U S outside U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please all
7. g the bronze button on the side of the pusher While holding the bronze button in slide the pusher to the left Raise the spring loaded syringe retainer and swing it out of the way Lay the loaded syringe in the V shaped holder Swing the retainer so it holds the syringe in place Move the pusher so it makes contact with the syringe plunger 5 Harvard Apparatus Pump 11 VPF Syringe Pump Operation Getting Started Getting started 1 Turn Pump ON Turn on power using the switch on rear of the pump the display will light and indicate POWER FAIL this is normal as the pump indicates on the dis play if power was disrupted since last use 2 Function Keys and Run Indicator Refer to the colored keypad at the front of the pump to identify the following functions starting from the right ra mm hr ul hr De commdia W A set diam Jenter stop run RUN STOP This turns the pump motor on and off ENTER This key enters the data that is on the display into the memory of the pump Also used to query the flow rate DIAM Used to enter or query the syringe diameter SET This key is used to select which digit of the display is to be changed to move the decimal point and to move between modes Each time the set key is pressed the underline cursor below the digit or character on the display moves one step to the right It
8. h any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 5 Harvard Apparatus Pump 11 VPF Syringe Pump Table of Contents SUBJECT PAGE NO General Information rrrrrrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnveveer 2 General Safety Summary annen 3 4 Introduction Theory of Operation annen eneen 5 FAUSA EE 5 Pump 11 VPF Front and Rear Views 6 Installation Initial Setup amp Location Requirments 7 Loading the S ide tn den de 7 Operation Getting Started Turn DONE soap annetd 8 Function Keys and Run Indicator 8 Entering Syringe Size a mrinensennninnseresnmwerntdee 9 Entering Flow Rate Rang nnen 9 Entering Flow Rate sje 9 Press PR M herean Aa bena 9 Check SING Aes S ren 9 Advanced Features Volume Mode sansen eaten 10 Power Failure ape setene ola 10 Changing Rates sne date wenn 17 M nt n o ved 11 Protecting Small Fragile Syringes 11 Appendices A Specifications add 12 B Table of Popular Syringe Diameters 13 C Flow Rates nnnn eneen eneen 14 Harvard Apparatus Pump 11 VPF Syringe Pump General Information Serial Number All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibration All syringe pumps are designed and manufactured to meet their performance spec ifications at all rated voltages and fr
9. low Rate Range Choose your flow rate units either microliters or milliliters per minute or per hour From the initial RATE VOL CONFIG menu using the A or V key move the underline cursor under the CONFIG mode menu prompt Press the SET key to enter the CONFIG mode Press the SET key again to move the under line cursor to the flow rate choices Choose your flow rate units while in the SET UNITS mode by pressing the A or V keys to scroll the four flow rate choices ml min l min ml hr pl hr Once the desired flow rate units are dis played press the ENTER key to return to the main SET CONFIG mode Press SET or ENTER again to put your desired flow rate units into memory and return to the RATE VOL CONFIG menu Enter Flow Rate From the initial RATE VOL CONFIG menu press the SET key to enter the SET RATE mode Each time you change the syringe diameter the previously used flow rate is erased If the syringe diameter is unchanged the previously used flow rate will appear on the display The underline cursor will appear under the left most digit or decimal point The A and V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired rate is displayed press the ENTER key to place this value into memory Press RUN Press the RUN STOP key to start pump and begin pumping The Run Indicator Green LED above the RUN STOP key will light when the pump is on and pumping Check
10. ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 Harvard Apparatus Pump 11 VPF Syringe Pump Appendix C Flow Rates nominal ml min diameter mm min max f min max min max min max Min 0 10 0 0001 1 241 0 0001 0 0207 0 0001 0 0012 1 pl 0 15 0 0002 2 793 0 0001 0 0465 0 0001 0 0027 2 ul 0 21 0 0004 15 476 10 0001 0 0912 0 0001 0 0054 5 ul 0 33 0 0009 13 52 0 0001 0 2253 0 0001 0 0135 0 0001 0 0002 10 ul 0 46 0 0016 26 27 0 0001 0 4379 0 0001 0 0262 0 0001 0 0004 25 ul 0 73 0 0040 66 16 0 0001 1 102 0 0001 0 0661 0 0001 0 0011 50 pl 1 03 0 0080 131 7 0 0002 2 195 0 0001 0 1317 0 0001 0 0021 100 ul 1 46 0 0161 264 7 0 0003 4 411 0 0001 0 2647 0 0001 0 0044 250 ul 2 30 0 0401 656 6 0 0007 10 94 0 0001 0 6566 0 0001 0 0109 1 000 ul 3 260 0 0806 1319 0 0 0014 21 98 0 0001 1 319 0 0001 0 0219 1ml 4 61 0 1611 2 638 0 0027 43 98 0 0001 2 638 0 0001 0 0440 2 5ml 7 28 0 4017 6 577 0 0067 109 6 0 0004 6 577 0 0001 0 1096 3 ml 8 66 0 5688 9 311 0 0094 155 2 0 0006 9 311 0 0001 0 1552 5 ml 10 30 0 8055 9 999 0 0134 219 5 0 0008 13 17 0 0001 0 2195 10 ml 14 57 1 610 9 999 0 0268 4393 0 0016 26 36 0 0001 0 4393 Max 35 00 9 289 9 999 0 1547 2 535 0 0 0092 152 1 0 0002 2 535
11. ow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts C A U T I O N Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as FOR RESEARCH USE ONLY completely as possible preferably using our part NOT FOR CLINICAL USE numbers If practical enclose a sample or drawing ON HUMAN OR We offer a complete reconditioning service VETERINARY PATIENTS CAUTION This pump is not registered with the FDA and is not for clinical use on human or veterinary patients It is intended for research use only Harvard Apparatus Pump 11 VPF Syringe Pump General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Power Supply The pump is supplied with an approved power supply and line cord To maintain the safety integrity of the device use only one of the following po
12. r appropri ate instructions Protecting Small Fragile Syringes The Pump 11 VPF will hold microliter size syringes down to 0 5pl size These small syringes have fine wire plungers that may be damaged if allowed to bot tom out The pump is equipped with an adjustable stop collar on one of the guide rods Use the provided hex wrench to position and lock the stop collar to prevent damage to small syringes 5 Harvard Apparatus Pump 11 VPF Syringe Pump Appendix A Pump 11 VPF Specifications Type Microprocessor single syringe infusion only Syringe Size for Pump 11 VPF with Holder For 2 Syringe Flow Rate Range Minimum Maximum Calibration Display Nonvolatile Memory Maximum Force Maximum Pressure Drive Motor Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Range Leakage to Ground Ground Resistance Input Power Input Power Connection Power Supply Size HxWxD Weight Plastic or glass From 0 5 ul to 10 ml 0 077 nl hr with 0 5 ul syringe 0 4393 ml min with 10cc syringe Automatic enter syringe size up to 35 mm Inside Diameter 2 line 16 character vacuum fluorescent display and green run led Stores diameter rate and configuration settings 25 Ib 100 p s i with 10cc syringe 175 p s i with 1 ml syringe 1 8 step angle geared 36 1 motor 1 pulse in 27 6 sec 200 steps sec 0 0444 um pusher advance per motor step 0 0388 u m min 0 833
13. ted Room Altitude up to 2000 m Mains Voltage Fluctuation not to Exceed 10 of Nominal Pump is Rated Pollution Degree 1 in Accordance with IEC 664 s Harvard Apparatus Pump 11 VPF Syringe Pump Introduction Theory of Operation The Pump 11 VPF is designed as a low cost infusion pump capable of low to mod erate back pressures The pump can hold two syringes of any make from 0 5 pl to 10ml The diameter of the syringes is entered via the keypad and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow Non volatile memory stores the last syringe diameter and flow rate along with other configuration data The Power Failure Mode can be set to either turn the pump off in the event of power failure or to resume pumping when power resumes Features Bright Display and Easy To Use Interface A new two line 16 character vacuum fluoressent display along with six membrane keys make this a most attractive but powerful easy to use syringe pump Only two entries required to start pumping syringe Inside Diameter mm and pumping flow rates The Flow rate can be changed while the Pump is running Two Modes of Operation Constant Flow Rate and Volume Dispense The Pump 11 VPF will operate continuously in RATE mode or accurately dis pense a specific amount of fluid in VOLUME mode Smooth Flow Enhanced micro stepping pump profiles deliver very smooth and consistent flow that is vir
14. tually pulse free Nonvolatile Memory The pump remembers its last syringe size flow rate used and configuration settings Power Fail Mode In a power failure the Pump can either RESUME or STOP pumping when power is returned CE Mark Approved The Pump 11 VPF meets all relevant European EMC and Safety requirements for laboratory equipment 4 Harvard Apparatus Pump 11 VPF Syringe Pump Pump 11 VPF Front and Rear Views PUSHER ALEN BLOCK HALF NUT WRENCH KEY PAD SYRINGE CLAMP SYRINGE HOLDER ADJUSTABLE STOP sf k srao CE 006313 REV 2 so WADE R us POWER CORD SERIAL SWITCH RECEPTICAL LABEL Harvard Apparatus Pump 11 VPF Syringe Pump Installation Initial Set up l Read the manual to become familiar with all features and functions of the Pump 11 VPE Connect the external DC power supply and line cord to the pump and main supply Turn on main power switch located on the rear panel The display will now illuminate indicating that the power connections are correct The display will indicate POWER FAIL this is normal as the pump indicates on the display if power was disrupted since last use Location Requirements for the Syringe Pump A sturdy level clean and dry surface Minimum of one inch 2cm clearance around the pump e Appropriate environmental conditions well ventilated room Loading the Syringe l A ae BS e DD Release the syringe pusher by pressin
15. using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET INTENSITY Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the SET INTENSITY choices 1 2 3 4 4 is highest intensity 1 is the lowest intensity Press the ENTER key to select the desired display intensity Exit the CONFIG mode by pressing the ENTER key again and save the INTENSITY setting in memory Changing Rates If the pump is running at an existing rate it will continue to do so until a new rate is entered Except for volume mode the flow rate can be changed while the pump is running s soon as the ENTER key is pressed the pump will change to the new flow rate To change rates from the keypad while in vol ume mode the pump must be stopped first Maintenance Keep the pump clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw and guide rods It is important to keep these guide rods clean and lubri cated To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the display window For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alco hol If the pump does not work properly contact Harvard Apparatus fo
16. wer supplies Ault Inc Part No PW118KA1203 Output 12 Vdc 1 5 A 18 W Input 100 250 Vac 47 63 Hz 5A 90 Vac Max Cui Stack Part No DTS120200U AC1 P6 Ouptut 12 Vdc 2 0 A Input 90 264 Vac 47 63 Hz 8A Max Input Globtek Inc Part No TR9CE1666LCP N Model GT 4201D 12 Output 12 Vdc 1 66 A 20 W Input 90 264 Vac 47 63 Hz 1A Max Use Proper Line Cord Use only the line cord shipped with the product and make sure line cord is certified for country of use Ground the Product This product is grounded through the return path of the DC power supply To avoid electric shock use only approved power supply and line cord with the product Make Proper Connections Make sure all connections are made properly and securely Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections 5 Harvard Apparatus Pump 11 VPF Syringe Pump General Safety Summary Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Functional Ground Refer to Manual Terminal Enviromental Conditions Indoor use only Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to 80 RH Well Ventila
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