MOOR INSTRUMENTS LIMITED Moorsoft for Windows for moorLAB
Contents
1. In the above window the serial port scroll slide display mode and monitoring schedule can be selected Note that for some computers a delay in the graphic display can be observed in scroll mode If this occurs slide mode should be used Note that in this version of moorLAB software only the free run and preset time modes are implemented others are greyed out 10 4 1 3 Set Up Display Configuration Once a monitoring window is open the display configuration can be defined by selecting Configuration Display Set up or clicking the icon The following window will be available Total Graphs 1 4 Active Graph 1 Unit Graph Label Graph d lid Graph 2 Concs Graph 3 needs Seas Gra i i apa Sape die Graphs in which the graph link status active graph graph labels graph units time scale and time offset can be changed The parameter traces which will be displayed in each graph can be defined by ticking the corresponding check box button Upto 4 parameters can be assigned to each graph If more than 4 parameters are chosen the first 4 will be used The time constants Y offsets and Y scales can be configured by clicking the Y and TC button in the above window The following dialogue box will be shown to allow user to change the settings Settings scales and ___2. Do not change this number If it is accidentally changed you will need to re enter it Contact Moor Instruments if help is needed Note if you are using a demonstration version this is fully operational but is a time limited version usually 3 months A message will be displayed on expiry Cannot Monitor Program has passed its expiry date use Configuration Registration Number to enter new registration number Contact Moor Instruments if you wish to purchase the software Moor Instruments will supply a registration number on receipt of your order N 4 STEP BY STEP GUIDE 4 1 Monitoring Window 4 1 1 Open a Monitor Window To open a Monitoring window select File New or click the icon E A monitoring window illustrated below will be shown u Laser Doppler Perfusion MOTO 1 0 Monitor 1 M 3 Speeds Time 00 00 00 00 00 05 00 00 10 00 00 15 00 00 20 00 00 25 4 1 2 Set Up Monitor Configuration To set up monitor configuration select Configuration Monitor Set up or click the icon E The following window will appear Settings for inonitor Serial Pot Display Mode COMM Port 1 Scroll Mode 7 COMM Port 2 ei j gt COMM Port 3 x Slide Mode gt Monitor Blood Concs t Monitor Duration Pause Duration Repeat indetinite gt Preset Mo Es E Free Run Indetinile gt Preset time Preset time
3. Graph YScale _Y Offset TC 50 Graph 2 50 or Graph 3 so vj a ort Graph 6 9 ie Graph 50 gt ie Graph 8 4 1 4 Configuration File The configurations described in sections 3 1 2 and 3 1 3 can be saved into a file Configuration gt Config File Save so that user can use the same configuration next time by loading the config file Configuration Config File Load 4 1 5 Start a Measurement Once a monitoring window is open and the moorLAB is in the RUN mode a measurement can be started by selecting Monitor Start or clicking the icon L A measurement can be Paused Continued by toggling the icon At the end ofa measurement selecting Monitor Abort or clicking the icon 22 will stop the measurement The recorded data can be saved by selecting File SaveAs or clicking the icon E There are two file formats available moorLAB format and text format 4 1 6 Icon Functions 4 8 6 9 10 1112 134415 16 17 18 19 The operations of individual elements of the icon tool bar are described below eal l ib open an existing mlb data file using standard windows Open Window 2 open a new graph window see Section 4 1 1 zi save data to disc There are two main options to save in MLB format Moor Instruments file or to save to standard TXT format file New data should always be saved into MLB format it c
4. menu i 3 INTRODUCTION 3 1 Laser Doppler Blood Flow Measurement Laser Doppler flowmetry provides easy to use non invasive real time measurements of local tissue blood flow Laser Doppler blood flow measurement uses the Doppler frequency broadening of laser light when the light is reflected off a moving object The degree of broadening is dependent on the speed of the moving object the wavelength of the laser light and the angle of the scatter For example laser light wavelength 780nm back scattered off a particle moving in a watery medium with a speed of 1mm sec has a frequency shift of approximately 3 3K Hz Laser light is used to illuminate the skin tissue resulting in some of the light being reflected straight back by the static tissue and some being reflected by moving blood The reflected light from the static tissue and the frequency broadened light from moving blood is collected and mixed on a photodetector The photocurrent can then be processed to produce indications of the flux concentration and speed of the moving blood The moorLAB floLAB uses solid state laser diodes as the laser light sources at about 780nm Two glass fibres are used mounted in a probe head one to transmit the laser light to illuminate the tissue and the other fibre to collect the reflected light This is then photodetected The signal from the photodetector is amplified and processed by an analogue processor The signal is then sampled and further p
5. tenente nhe 10 4 1 3 Set Up Display Configuration 11 4 1 4 Configuration File i d ea ei a aA anaes 12 4 1 5 Measurement uice eo eee thas puerile lodi ee por We pea sce loce 12 4 1 6 Icon Functions iaa re e et er ede Arten e ue a Ee deno 13 4 1 7 Monitor Window Functions 18 4 2 REVIEW WINDOW PE 19 4 2 1 Open a Review Window Snnt ett BE eer el tee eed 19 4 2 2 Icon FUnctions xi ni eni a 19 2 wu Moor Instruments Limited assumes no responsibility for the operator s failure to comply with instructions given in this manual 1 USING THE moorLAB USER MANUAL This manual is for use with Moor Instruments Moorsoft for Windows for moorLAB floLAB software run on an IBM 486 PC Pentium or compatible in conjunction with the moorLAB floLAB blood flow monitor Users unfamiliar with laser blood flow monitoring and analysis should read the Introduction of this manual Otherwise users need only read the section Step by Step Guide 2 NEW FEATURES COMPARED WITH V1 0 This version a Does not require a dongle key to operate see Section 3 3 3 Registration Number b Can display graphically either conc or dc values and stored values of either conc or dc Version 1 0 did not allow display and reading of dc Has analogue outputs of flux to 4 and either conc 1 to 4 or dc to 4 selectable from the moorLAB LCD displayed
6. MOOR INSTRUMENTS LIMITED Moorsoft for Windows for moorLAB V1 3 USER MANUAL Issue 2 A data handling program for use with moorLAB Issued 12th November 2001 Moor Instruments Limited Millwey Rise AXMINSTER Devon EX13 5HU England Tel 44 0 1297 35715 Fax 44 0 1297 35716 Website www moor co uk Email marketing moor co uk 1 USING THE MOORLAB USER MANUAL sne soto sens 3 2 NEW FEATURES COMPARED WITH V1 0 cssescssscescercescesversrscesessvesseasescsssssosnsssnsessesosenesaes 4 3 INTRODUCTION P 5 3 1 LASER DOPPLER BLOOD FLOW 5 3 2 OVERVIEWOEMOORLA B rr PE HOS ee pe Rie ea ee SERRE DEN 7 3 3 THE COMPUTER AND SOFTWARE INSTALLATION nennen nennen rnnt nennen 7 3 3 1 Computer Specificallons ec rre i na evel 7 3 3 2 Sofrware noe tb ce i tete y EE b ge 7 3 3 3 Registration sekro teta t tete renidet re tte pete eee abre EARE o 8 4 STEP BY STEP GUIDE P 9 4 1 MONITORING WINDOW 532221 22 rentre eU Ede Feeds teo Pepe eb arre e Dee eria do ede paganis 9 4 1 1 Open a Monitor Window arne eaa a E E N E N R a 9 4 1 2 Set Up Monitor Configuration eese
7. an only be reviewed by Moorsoft for Windows moorL AB if in this format TXT format is to enable moorLAB data to be viewed by spreadsheet and other data processing software The options are available from the following window Content of Save FILE TYPE _ FORMAT 5 MLB AB Format saving all data e ASCII Format specifically saving parameters TXT yi i FY DG F2 DC2 F3 DC3 F4 DC4 n 3145 3 J i during 4 the displayed page only all of the trace Buc N B This will build a table of 77 rows The ASCII file size will be up to 5 3 KBytes TXT Option This enables the user to choose which parameters are saved from F2 DC2 F3 DC3 F4 DC4 or CONC values instead of DC values depending on what has been selected on the moorL AB at run time The data segment saved can be the displayed page only or all of the trace When displayed page only is selected it is saved at the data rate being currently displayed on the PC screen When all of the trace is selected there is a choice of sampling rates 20 30 40 per hour 1 2 4 8 10 15 20 30 40 per minute and 1 2 4 8 10 20 40 per second The number of data rows and an estimate of the ASCII file size is also given 14 N B Values saved in the text file are averages of data in the period following the nominal time point e g for data stored at 5Hz the
8. ctangle will toggle between link un link of the graphs If L is displayed in the rectangle all graphs are linked i e changing Y scale Y offset and time constant will affect all graphs If U is displayed in the rectangle all graphs are not linked i e changing Y scale Y offset and time constant will only affect the active graph 00 00 00 00 00 05 00 00 10 00 00 15 00 00 20 00 00 25 X scroll bar Y scroll bar Active graph clicking on the numbered rectangle or on the graph rectangle will make that graph as the active graph make the previous mark left to the current active mark as the active mark gt gt make the next mark right to the current active mark as the active mark X scroll bar it can be used to change time offset Any X scroll bar action will affect all graphs Y scroll bar it can be used to change Y offset If all graphs are linked any Y scroll bar action will affect all graphs otherwise it will only affect the active graph 18 4 2 Review Window 4 2 1 Open a Review Window Data stored in the moorLAB format can be loaded me S review window To open a review window select File Open or click the icon e If the file is successfully opened the parameter traces will be displayed in the same configuration as when they were saved An example is illustrated below 1 Channel 1 2 Channel 2 3 Channel 3 4 Channel 4 Time 00 00 00 00 00 02 00 00 04 00 00 06 00 00 08 00 00 10 Pl
9. ctive graph Fi 250 2030 f se 70 4 pq eue spe si Total Point 86 Start point 24sec Endpoint 4 5 sec Active Graph Number 1 TC off In both situations a File menu will be available to allow user to save or print the results 2 start a measurement an stop a measurement toggle pause continue a measurement expand time scale compress time scale t expand Y scale compress Y scale TC increase time constant smooth TC decrease time constant I insert a mark In a real time monitoring mode when the mark icon is clicked a mark will be added in real time The added mark will be defined as the active mark which is displayed by a black rectangle in the mark bar The mark label can be entered in the mark label Edit refer to the diagram on next page If real time monitoring is aborted or paused clicking the insert mark icon will add a mark in the cursor position and open an edit mark notes dialogue box so that user can enter mark label and comment as illustrated below Mark Notes Information pite Comment delete the current active mark e print the parameter traces displayed in the current window after a measurement q open the help menu The commands help gives explanations for all menu items The Toolbar help explains all icon functions Monitor Window Functions Link status clicking the link status re
10. ease refer to section 3 1 3 and section 3 1 4 for configuration set up 4 2 2 Icon Functions The icon functions in a review window are the same as in a monitor window refer to section 3 1 6 except that the icons gt S and not available in a review window
11. result saved at the 5 second time point is the average of 40Hz values between 5 and 5 2 seconds set up monitor configuration see Section 4 1 2 E 3 set up display configuration see Section 4 1 3 calculate statistics within a selected region To define a statistics region press down the left mouse button at the beginning of the region followed by dragging the mouse with the left button still pressed to reach the end of the region and releasing the mouse button The selected region will be shown in the inverted colours as illustrated below 1 Channel 1 4 Channel 3 Channel 3 4 Channel 4 Time 00 00 00 000002 00 00 04 00 00 06 00 00 08 00 00 10 Once the region has been defined clicking the statistics icon will bring up an option dialogue box so that user can choose between calculating the statistics for all selected parameters raw data only and calculating statistics for parameters in the active graph only time constant considered If the first option is chosen the statistics will be shown as follows 15 Statistics example mib ci j 230 36 194 203 598 70 asa jan e 1521 187 115 19 15 2 125 38 105 10 m2 2 h 69 0 53 80 A 95 102 7 17 9 27 66 Total Point 86 Stat pomt 2 4 End pomt TC OFF If the second option is selected the following table will be given as graph is the selected a
12. rocessed by a digital processor The digital processor also performs all the user interface and display functions The mean blood cell flux flux number concentration of moving blood cells conc mean speed of the blood speed of each moorLAB floLAB channel are output to a PC in real time via an RS232 serial link If moorLAB floLAB is running on a PC this information may be collected displayed stored analysed and converted into moorLAB format or text format The algorithms used to compute flux conc and speed are as follows kif P o do dark short noise s dc i2 dark short noise dc conc speed k3xflux conc w is the frequency of Doppler shift 2 frequency in Hz P w is the power of signal at frequency w dc is the intensity of all detected light is the low cut off frequency 2nfj frequency in Hz w7 is the high cut off frequency 05 2nf2 f2 frequency in Hz kj and are scaling constants The optical units are arbitrary The values assigned are determined by standardising the moorLAB floLAB parameter of flux blood flow etc using a physical standard e g the thermal Brownian motion of polystyrene microspheres sub micron diameter sphere in water The laser Doppler monitor user guide should be consulted for information on calibration 3 2 Overview of moorLAB The moorLAB program receives the moorLAB floLAB real time flux and conc data
13. via the RS232 serial link and converts the information into a graphical format The data will be displayed in real time and stored The moorLAB software consists of two different windows they are Monitoring window receive and display real time parameter signals from moorLAB floLAB Review window load and analyse saved parameter data from a moorLAB format file 3 3 The Computer and Software Installation 3 3 1 Computer Specifications Only computers complying with EC 950 should be used The minimum requirements for running the moorLAB software is Processor 486 dx PC Pentium is recommended Memory 4Mb RAM SOMb space on hard disk drive depending on monitoring duration minimum recommended Operating system Windows 3 11 or higher e g Windows 95 3 3 2 Software Installation A working knowledge of Windows is assumed for installation and operation of moorLAB software To install place the disk in the drive A From Program Manager select File then Run Type A INSTALL Then follow the instructions 3 3 3 Registration Number A registration number has been assigned to the copy of moorLAB that has been supplied to you This number is automatically recorded at installation and can be displayed during RUN Click File New Configuration Lasei Doppler Peifusion Mo xal File Edt Monitor Analys Config File Monitor Setup Display Setup Biegistation Number
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