EdvoCycler™ Quick Guide
Contents
1. 337 952 961 962 PROGRAM 330 Init Denat Denaturation Annealing Extension Final Ext Cycles PROGRAM gt 000 Init Denat Denaturation Annealing Extension Final Ext Cycles 341 701 004 371 764 TEMP 94 45 72 72 01 TEMP 00 00 00 00 00 00 TEMPERATURE HOLD MENU TEMP HOLD ON gt SET HOLD TEMP RETURN EDVOTEK 1 800 EDVOTEK 010 that selection The five options are as follows LOAD SAVED PROGRAM MODIFY SAVED PROGRAM CREATE NEW PROGRAM DELETE SAVED PROGRAM RUN PROGRAM LOAD SAVED PROGRAM Use this function if you are running an EDVOTEK kit The programs for most EDVOTEK PCR kits are pre loaded on the EdvoCycler and ready to run Simply follow EDV OTEK instructions for preparing DNA samples for PCR and place tubes in the EdvoCycler The block holds only 0 2 ml PCR tubes if PCR sample is prepared in a different tube you must transfer sample to a 0 2 ml thin wall PCR tube supplied in all EDVOTEK PCR kits IMPORTANT Always check the PCR cycling parameters in the EDVOTEK manual that accompanies your experiment Compare the program to ensure that it matches the pre programmed cycles If there has been a change it will be necessary to modify the existing program to match the new parameters 1 Scroll to LOAD SAVED PROGRAM and press enter 2 Scroll up or down to the desired program and press enter Check with the kit instructions to make sure2. enter here to go back to main menu NOTE Running the thermal cycler on hold for long periods of time will shorten the life of the unit EDVOTEK 1 800 EDVOTEK www edvotek com info edvotek com 8 EdvoCycler RUN SAVED PROGRAM This option will run the last program entered 1 Scroll to RUN SAVED PROGRAM and press the enter button The last program entered will begin cycling PAUSE or ESCAPE Use this option to pause or end a program 1 While a program is running push PAUSE to temporarily hold a cycle at a certain point USE EXTREME CAUTION WHEN OPENING THE LID AND REMOVING SAMPLES FROM THE BLOCK THE HEATED LID AND BLOCK WILL BE VERY HOT 2 When ready to return to the program replace samples to the block close the lid and push the RUN button 3 To stop the program while it is running press the escape button Press the up or down button to YES and press enter to abort the program If at any point the unit freezes up turn power off at the switch located at the back wait a few minutes to allow the unit to cool and turn it back on EDVOTEK 1 800 EDVOTEK www edvotek com info edvotek com
3. EdvoCcycler Catalog 541 ENXACNMIME Bul Lvl Rule ENGAGE Features 25 x 0 2 ml Tube Block Heated Oil Free Lid with Magnetic Latch Vivid 7 Line LCD Display Pre Programmed PCR Protocols Temperature Range 4 99 C Maximum Ramp Rate 3 C sec Weight amp Dimensions 11 Ibs 16 x 8 5 x 7 2 Year Warranty Made in USA TABLE OF CONTENTS Introduction Warnings Operator Safety Installation After Use User Maintenance Guarantee Contact Information WWWNHNNNN EdvoCycler Quick Guide Getting Started Load Saved Program Modify Saved Program Create New Program Delete Saved Program Temp Hold Params Run Saved Program Pause or Escape WOONNDAUUA EDVOTEK 1 800 EDVOTEK www edvotek com info edvotek com M 2 EdvoCycler Introduction Please read all the information in this booklet before using the unit Warnings HIGH TEMPERATURES ARE DANGEROUS they can cause serious burns to operators and ignite com bustible material EDVOTEK has taken great care in the design of these units to protect operators from hazards but users should pay attention to the following points USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS DO NOT put hot objects on or near combustible objects DO NOT operate the unit close to flammable liquids or gases DO NOT place any liquid directly in your unit At all times USE COMMON SENSE Operator Safety It is important that only sui
4. REAR PANELS EXPOSES POTENTIALLY LETHAL MAINS VOLTAGES THERE ARE NO USER MAINTAINABLE PARTS WITHIN THE EQUIPMENT In the unlikely event that you experience any problems with your unit that cannot easily be remedied you should contact EDVOTEK to explain the problem and obtain a Return Goods Authorization After obtaining the RGA return the unit if necessary and include any details of the fault ob served Remember to return the unit in its original packing EDVOTEK accepts no responsibility for damage to units that are not properly packed for shipping See the Decontamination Certificate supplied with your unit 1 Cleaning Before cleaning your unit ALWAYS disconnect from the power supply and allow to cool below 50 C The unit can be cleaned by wiping with a lightly damp soapy cloth Care should be exercised to prevent water from running inside the unit Do not use abrasive cleaners 2 Fuses The unit is protected by one fuse This fuse should only be changed by suitably qualified person nel If the fuse blows persistently a serious fault is indicated and you may need to return the unit for repair The fuse is located in the back of the unit in the AC adapter Guarantee The unit is guaranteed against any defect in material or workmanship for two years Please fill out the warranty card as soon as possible to ensure proper warranty coverage The warranty period is from the date of purchase and within this period all defective
5. empera ture time and number of cycles scroll to value to be changed press ENTER Using keys 0 9 punch in desired value NOTE need to keep same number of digits so if 45 sec enter 0045 Once finished ESCAPE back to MAIN MENU NOTE There are only 20 program slots if you create a program in addition to 20 the last one will be deleted Use DELETE SAVED PROGRAM to create a space for a new program when all program space is full Press ENTER to select one of 20 saved programs Press ENTER to choose and select YES or NO Once complete you will automatically be placed back into the MAIN MENU Select the hold temperature by pressing ENTER then use the UP or DOWN keys to choose between 10 94 Press ESCAPE when the desired temperature is selected Scroll to RETURN and press ENTER to go back to MAIN MENU NOTE The heated lid shuts off when the unit finishes a program and goes to hold WARNING Running the unit for long periods of time at high or low temperatures will shorten the lifespan of the unit www edvotek com info edvotek com EdvoCycler i GETTING STARTED Quick Guide Screens n Turn on the power switch mark is on O mark is off Use the up or down button on the front panel to select the desired pst function the arrow to the left of the menu will allow you to choose gt LOAD SAVED PROGRAM MODIFY SAVED PROGRAM CREATE NEW PROGRAM DELETE SAVED PROGRAM TEMP HOLD PARAMS LOAD PROGRAM 336
6. ion TEMP and enter the desired temperature Press enter at the Exten sion TIME and enter the desired time 9 Ifa final extension is not desired scroll down to the Cycles default see below for entering the number of cycles 10 If a final extension is desired press the enter button and enter the final ex tension temperature Next press the enter button again and enter the final extension time NOTE Time is entered ds Finally press the enter button at the Cycles default siento For example Enter the number of cycles for the PCR cycling steps 30 sec 0030 The number to the right is the total number of cycles and the number to the left 2 min 0120 will display as the current cycle in progress DELETE SAVED PROGRAM The EdvoCycler can hold 20 saved programs From time to time it may be necessary to delete old programs to make room for new programs Remember once a program is deleted it cannot be retrieved You will need to re enter the program you may want to record the PCR cycling steps for programs that are deleted 1 Scroll to DELETE SAVED PROGRAM and press enter 2 Scroll to the program you wish to delete and press enter When prompted ARE YOU SURE press enter YES to delete the program or scroll up or down to enter NO to keep the program TEMP HOLD PARAMS From the Main Menu TEMP HOLD PARAMS Enter into that menu e Temp Hold scroll for on off e Set Hold Temp 4 C 10 C e Return
7. parameters match with screen display If they do not reference MODIFY SAVED PRO GRAM section and make the necessary changes to the existing program www edvotek com continued info edvotek com 6 EdvoCycler LOAD SAVED PROGRAM continued Warning 3 Load tubes in block If all spaces will be used it may be necessary to nest tubes place tubes aligned with one another and facing the same direc The heated lid tion See Figure 1 Close lid will get very hot use extreme caution to avoid getting burned 4 Press the RUN button The lid starts to preheat or the cycling will begin im mediately 5 When the cycling steps begin you can monitor the progress on the screen display The flashing arrow indicates the current status The bottom row Cycles shows current cycle to the left and the total of cycles to the right ss 6 To end the cycling at any time Press the escape button and scroll with the OO up or down button enter YES to stop the experiment C MODIFY SAVED PROGRAM OC Figure 1 Q R a XY G Use this function to modify or to make an adjustment to an existing program 1 Scroll to MODIFY SAVED PROGRAM and press enter 2 Scroll up or down to the desired program and press enter 3 Scroll to the setting you wish to change and press enter When the item disappears enter the new parameter 4 Scroll to another setting you wish to modify After you are satisfied with the change
8. parts will be replaced free of charge provided that the defect is not the result of misuse accident or negligence Servicing under this guarantee should be obtained from EDVOTEK Notwithstanding the description and specification s of the units contained in the User s Manual EDVOTEK hereby reserves the right to make such changes as it sees fit to the units or to any component of the units This Manual has been prepared solely for the convenience of EDVOTEK customers and nothing in this Instruction Book shall be taken as a warranty condition or representation concerning the description merchantability fitness for purpose or otherwise of the units or components Warning Please note that the keypad on the EdvoCycler is easily damaged by sharp objects such as pens pencils and fingernails Damage of this sort will be considered as misuse and will invalidate the guarantee on this unit Contact Information For technical sales or servicing information contact EDVOTEK Inc PO Box 341232 Bethesda MD 20827 Telephone 1 800 EDVOTEK Fax 301 340 0582 email edvotek aol com Web site www edvotek com EDVOTEK 1 800 EDVOTEK www edvotek com info edvotek com EDVOTEK M EdvoCycler EdvoCycler Quick Guide MAIN MENU Screen A Move cursor UP and DOWN select by pressing ENTER escape by pressing ESCAPE LOAD PROGRAM Screen B Select one of 20 saved programs by using UP or DOWN and pressing ENTER Modify Saved P
9. rogram Select one of 20 saved programs by using UP or DOWN and pressing ENTER Create NEW Program Delete Saved Program TEMP HOLD PARAMS Screen E Use to select whether or not you want the unit to keep the block at a constant tempera ture after an experiment is complete Press ENTER for YES or NO 1 800 EDVOTEK PROGRAM SCREEN Screen C Shows temperatures times and number of cycles Press RUN to start Once running press PAUSE to pause P will flash by the time Press RUN again to continue To abort the program while running press ESCAPE Then choose YES or NO Scroll UP or DOWN to choose NO will return to experiment YES will return to MAIN MENU IMPORTANT Always check the PCR cycling parameter in the EDVOTEK manual that accompanies your experiment Compare the program to ensure that it matches the pre programmed cycles If there has been a change it will be necessary to modify the existing program to match the new parameters To modify the temperature time and number of cycles scroll to value to be changed press ENTER Using keys 0 9 punch in desired value NOTE You will need to keep same number of digits so if 45 sec you will need to enter 0045 Can enter 001 999 for program numbers 1 60 for of cycles 15 99 for tempera ture and O 1800 seconds for time Screen D Once finished ESCAPE back to MAIN MENU Change values by pressing ENTER and keying in numerical values To modify the t
10. s to the program press the escape button and the changes will be saved Time is entered as seconds i e 30 seconds is displayed as 0030 2 minutes is displayed as 0120 5 Torun the modified program scroll to LOAD SAVED PROGRAM and follow the directions running a saved program CREATE NEW PROGRAM Note if an incorrect entry is made at any time during CREATE NEW PROGRAM simply scroll up or down to the wrong entry and press enter When the item disappears enter the correct param eter 1 Scroll to CREATE NEW PROGRAM and press enter 2 Press the enter button and then enter the new program number 3 If an initial denaturation is not desired scroll down to Denaturation see below for entering PCR cycles 4 If an initial denaturation is desired press the enter button and enter the Initial Denaturation temperature Next press the enter button again and enter the Initial Denaturation time i e for 3 min enter the time as 0180 continued EDVOTEK 1 800 EDVOTEK www edvotek com info edvotek com EdvoCycler CREATE NEW PROGRAM continued 5 Enter the three PCR cycling steps 6 Press enter at the Denaturation TEMP and enter the desired temperature Press enter at the Denaturation TIME and enter the desired time i e for 30 seconds enter the time as 0030 7 Press enter at the Annealing TEMP and enter the desired temperature Press enter at the An nealing TIME and enter the desired time 8 Press enter at the Extens
11. tably trained personnel operate this equipment in accordance with the instructions contained in this manual and with general safety standards and procedures If the equipment is used in a manner not specified by EDVOTEK the protection provided by the equip ment to the user may be impaired All EDVOTEK units have been designed to conform to inter national safety requirements and are fitted with an over temperature cutout The cutout is preset to protect the unit If a safety problem should be encountered switch off at the mains socket and remove the plug from the supply Installation 1 All EDVOTEK units are supplied with a power cable 2 Plug the main cable into the socket on the rear of the unit 3 Place the unit on a dry suitable bench or flat work space ensuring that all air inlet vents are free from obstruction and liquid 4 Symbols on or near the power switch of the unit have the following meanings main switch On O main switch Off After Use Warning When you have finished processing samples remember that parts of the unit The heated lid the tubes blocks and associated accessories may be very hot Take pre will get very hot caution to avoid potential burns use extreme caution to avoid getting burned EDVOTEK 1 800 EDVOTEK www edvotek com info edvotek com EdvoCycler 2 User Maintenance NOTE THIS EQUIPMENT SHOULD ONLY BE DISMANTLED BY PROPERLY TRAINED PERSONNEL REMOVING THE SIDE FRONT OR
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