Laboratory Sterilization System INTRODUCTION
Contents
1. Use an air conditioner or dehumidifier to lower humidity levels before using the sterilizer g Do not attempt to operate the Ozilla Sterilizer in an enclosed space when people or animals are present ozone gas may cause respiratory irritation at higher concentrations make sure the Ozilla device finishes its sterilization and de ozonation scrub cycles in full h Do not operate this appliance if it has a damaged cord or plug if it is not working properly or if it has been damaged or dropped _ Oo c x x i Do not immerse cord or plug in water keep cord away from heated surfaces j Do not attempt to repair or open the unit unless you are a qualified repair technician SPECIFICATION 1 year parts and labor 1 year parts and labor WARNING Not all materials should be sterilized with Ozilla property damage can result Ozilla is intended for use in laboratories and on some laboratory equipment to eliminate airborne and surface contaminants and germs Different materials respond differently to ozone gas and some common laboratory materials may be damaged by ozone exposure To minimize property damage please carefully read and adhere to the guidelines below Genlantis is not responsible for damage arising from failure to follow instructions relating to Ozilla s use Guidelines SE Strong effect Materials are most susceptible to ozone gas degradation these materials should be removed from areas to be ste2. Ozilla Sterilizer as close as possible to the contaminated area the ozone concentration is highest as it exits the unit e Place the Ozilla Sterilizer at the highest possible point in a room or cabinet ozone is heavier than air and will drift down and neutralize contaminants in its path e Use existing air currents or circulating fans whenever possible or available to help disperse the ozone gas as much as possible Preparing the Area To Be Sterilized 1 Clean area or cabinet that needs sterilization from heavy or excess contaminants using appropriate cleaning solutions like soap ethanol bleach etc NOTE ozone gas is able to safely and effectively sterilize airborne or surfaces contaminants however the gas will not penetrate and clean liquids and solutions or contaminants hidden from the gas Water inhibits the high level generation of Ozone gas 2 Remove any liquid from the area to be cleaned by the Ozilla Ozone Sterilizer Place the ozone sterilizer in a central and high location in the space that needs sterilization Seal off any gaps in the unit or area to be sterilized This is critical to get a high level of ozone gas buildup to sterilize the area that is exposed to the ozone gas 3 If sterilizing a room large openings or holes to create an air tight space and prevent ozone gas from escaping It is necessary to seal openings in intended sterilization space with plastic sheeting and or tape 4 Donotuse the Ozilla Sterilizer in area
3. Ozilla Ozone Sterilizer Scentantis Laboratory Sterilization System A division of Gene Therapy Systems Inc Catalog Description Content E400110 Ozilla Ozone Sterilizer 110V 1 Ozilla Device 110V 1 Ozilla Scrub Filter 1 Power Cord 1 Spare Fuse E400220 Ozilla Ozone Sterilizer 220V 1 Ozilla Device 220V 1 Ozilla Scrub Filter 1 Power Cord 1 Spare Fuse Shipping and Ozilla is shipped at room temperature Store on a lab Storage bench away from water and heat sources INTRODUCTION The Ozilla amp Sterilizer is a unique and innovative laboratory sterilization system This portable bench top sterilizer from Genlantis uses high levels of ozone gas to clean and sterilize laboratory work cabinets hoods incubators etc and various tools especially ones involved in sensitive experiments or assays and that cannot be sterilized with standard heat autoclaves The Ozilla Sterilizer will eliminate T surface or airborne bacteria mold fungi spores and viruses and eliminate odors when used correctly The device is small enough to put directly inside cell culture incubators tissue culture hoods or any other sealed enclosures that need to be Sterilized or kept sterile When used with the Ozilla Ozone Sterilizer Nest available separately under catalog E40010B the Ozilla Sterilizer can be used to clean a variety of equipment and tools especially ones that will be damaged or destroyed in a standard autoclave such as pipettes pipe
4. VC Cross linked polyethelene PEX ehtylene propylene Ethylene acrylic elastomer Vamac Ethylene propylene rubber EPR Fluorosilicone Glass High density polyethylene HDPE Perfluoroelastomer Kalrez Chemraz Viton Polycarbonate Polychlorotrifluoroethene PCTFE Polyether ether ketone PEEK Polytetrafluoroethylene PTFE Polyurethane PVDF Silicone Stainless Steel Superalloys Hastelloy Inconel Thermoplastic elastomer TPE Titanium lt x X X X xX x X KKK KK KK KKK KK XX X KKK KKK KKK KKK KK KKK X X XK Hastelloy C is a trademark of Haynes International Chemraz is a registered trademark of Green Tweed and Co Tygon is a registered trademark of Saint Gobain Corporation Monel is a registered trademark of Special Metals Corporation Delrin Hypalon Hytrel Hypalon Polychloroprene Vamac Viton and Kalrez are trademarks of DuPont VKM140808 Genlantis Page 3 of 7 11011 Torreyana Road San Diego CA 92121 Toll Free 888 428 0558 U S amp Canada 858 457 1919 www genlantis com 2014 Genlantis and Gene Therapy Systems Inc Ozilla Ozone Sterilizer Manual OPERATION Positioning the Unit The Ozilla amp Ozone Sterilizer is portable and ready to plug in and use directly out of the box Its effectiveness depends on the amount of ozone gas that is generated and how it dispersed through ambient air For optimal sterilization follow these simple recommendations e Place the
5. apy water ddis plates araa Make sure it is completely dry before reinstalling it TELELLLLLELLEELLLLLLLL LIE NOTE As a safety mechanism the Ozilla Sterilizer e Front will not operate if the small grill is missing or the thumb screw is not reinserted fully into the unit Figure 2 Top view of Ozilla Ozone b Scrub Filter Replacement Replace the scrub filter once every 3 000 hours of operating the unit or 9 months whichever comes first by removing the large grill on the top of the unit NOTE As a safety mechanism the Ozilla Sterilizer will not operate if the scrub filter is absent or the thumb screw is not reinserted fully into the unit c Ozone Plates Cleaning Clean the ozone plates at the beginning of each new season or when you suspect that ozone production has decreased To clean the ozone plates remove the small grill on top of the unit and pull out the two white ceramic generator plates i wash the plates in warm soapy water and air dry or use a hair dryer ii clean the contact area of the flat stainless steel springs with a Q tip moistened with alcohol and dry with tissue or let air dry iii to avoid arcing or damaging the Ozilla Sterilizer make sure plates are completely dry before re inserting into the unit NOTE As a safety mechanism the Ozilla Sterilizer will not operate if the small grill is missing or the thumb screw is not reinserted fully into the unit d Ozone Plates Replacement Replace every yea
6. attached on the back of the unit SCRUB TIME buttons are pressed e Check that both thumb screws that secure the large and small grill plates on top of the unit are fully screwed in e Sometimes during shipping the ozone ceramic plates may get dislodged Check the plates and seat them properly by removing them and reinserting them back tightly in their respective slots Ozone smell is not as noticeable as usual Clean the ceramic ozone plates as described in the MAINTENTANCE section If plates are older or there is no detectable ozone smell than one year replace with new ones Cat E40010P Check fuse on the back side of the unit and if blown replace with type 239 250V 3 Amp fuse VKM140808 Genlantis Page 5 of 7 11011 Torreyana Road San Diego CA 92121 Toll Free 888 428 0558 U S amp Canada 858 457 1919 www genlantis com 2014 Genlantis and Gene Therapy Systems Inc Ozilla Ozone Sterilizer Manual MAINTENANCE B ack Hill Hn i Scrub filter nut el TELEELLELLELLELLELLLELLLLL LIT WARNING The O2zilla Ozone Sterilizer generates high voltage current inside the machine MAKE SURE TO UNPLUG UNIT from power outlet before performing any parts cleaning or replacements a White Filter Cleaning periodically clean the white dust filter located under the small grill on the top of ete tete the unit Remove the thumb screw lift the plate and White filter and peel off the filter Wash it in warm so
7. b cycle the digital display will go blank before entering space or opening cabinet that is being sterilized Name Date Time Sterilization Cycle Started Expected Cycle Completion Photocopy this page of the manual and post on the door of an area or cabinet during every ozone sterilization cycle
8. closure door or window to seal the cabinet that is being sterilized or if sterilizing a room exit and close the door behind Upon sterilization and scrub cycles completion the Ozilla Sterilizer will automatically turn off digital display will be blank users can enter the room or open the cabinet that is being sterilized If needed the Ozilla Sterilizer can be run again with current or readjusted settings for further cleaning cycles For units with the optional remote control E4001RC The Ozilla Remote Control allows users to switch the Ozilla Sterilizer RUN cycle from Ozone to Scrub This allows users to immediately terminate the sterilization cycle short of the programmed time by turning the Scrub cycle on By doing that the Ozilla Sterilizer will start to remove the ozone gas that has been generated by the unit and will run the Scrub cycle for the amount of time that has been programmed by the user Table 1 Recommended Sterilization and Scrubbing Times Based on Size of Cabinet or Area Incubator Tissue Culture Hood 3 feet wide 24x 283 x 2 13 5 ft Tissue Culture Hood 4feetwide 24x 39 x2 18 7 ff Tissue Culture Hood 5 6 feet wide 24x4 9 x 2 23 5 ft 8 x 12 x 10 960 ft Ozilla Ozone Sterilizer Nest NOTE Minimum Scrub Times recommended can be extended by running additional scrub cycles if needed or desired TROUBLESHOOTING Unit does not start when OZONE TIME or e Check that power cord is
9. inants especially bacteria and fungus that exist under normal use in laboratory areas Heavier concentrations of contaminants should be removed or wiped clean first before using the Ozilla sterilizer CAREFULLY READ AND FOLLOW ALL INSTRUCTIONS FOR OPERATION OF THE OZILLA STERILIZER AVOID walking into any room or enclosure while the Ozilla Sterilizer is still running AND before it completes its full sterilization and de ozonation cycles TGenlantis makes no claims and offers no guarantees of any kind that the Ozilla Sterilizer will eliminate 100 of all contaminants under all circumstances Whenever and wherever possible users should test an Ozilla sterilized area for presence of trace contaminants if absolute cleanliness is desired or required PRECAUTIONS AND WARNINGS a DEVICE IS INTENDED FOR CHEMICAL OXIDATION AND DISINFECTION IN LABORATORIES IN THE BIOTECHNOLOGY PHARMACEUTICAL CHEMICAL AND ELECTRONICS INDUSTRIES b DEVICE IS NOT INTENDED FOR USE IN OCCUPIED SPACES c DEVICE NOT INTENDED FOR CLEANING AIR NEAR A PERSON IN RESIDENTIAL OR COMMERCIAL SPACES MOTOR VEHICLES OR OCCUPIED SPACES Ozilla Ozone Sterilizer Manual Read all instructions before using the appliance This device must be used ONLY as intended and described in this manual Do NOT operate the Ozilla device without the filter grills inserted and fastened on top of the Ozilla device f DO NOT Immerse the Ozilla Sterilizer in water or operate at humidity levels above 60
10. ll blink c Press SET TIME up or down arrow button repeatedly until desired time is selected d Press TIME SELECT button to move to next digit repeat Step c then press SET TIME to move to the next digit e Repeat Step d one last time to set the last digit the display now should read the ozone time with no blinking digits VKM140808 Genlantis Page 4 of 7 11011 Torreyana Road San Diego CA 92121 Toll Free 888 428 0558 U S amp Canada 858 457 1919 www genlantis com 2014 Genlantis and Gene Therapy Systems Inc Ozilla Ozone Sterilizer Manual To set the run time for the scrub cycle For recommended scrubbing times based on size of area see Table 1 below Press the SCRUB TIME button the digital display will read either OOOO or a previously set time Press TIME SELECT button the left most digit will blink Press SET TIME up or down arrow button repeatedly until desired time is selected Press TIME SELECT button to move to next digit repeat Step c then press SET TIME to move to the next digit Repeat Step d one last time to set the last digit the display now should read the ozone time with no blinking digits m 3J Co Fr k Once the ozone and scrub cycles have been set press the RUN button the green light will turn on and you will hear the machine begin its cycle run If needed or desired set a lab timer with the total sterilization and scrubbing cycle times as a reminder Close the en
11. r or two especially when you notice a decrease in cleaning efficiency or a decrease in ozone gas smell Follow the procedure described in c to remove and replace the ceramic ozone plates e General cleaning wipe the Ozilla Sterilizer with a clean soft cloth tissue that s lightly moistened with water of mild cleaning solution DO NOT SPRAY the unit directly with any liquid cleaner or immerse in water or solution of any kind FIRST AID if users experience eye or breathing irritations immediately take the following steps i Turn the sterilization cycle off by pressing RUN button l ii Remove affected person from the area and administer first aid as necessary and described in the Material Safety Data Sheet 1 iii Run a scrub cycle as indicated in this Owner s Manual Section III Operating the Unit on Page 3 alternatively turn the Ozilla I I l device off and air the affected area until the odor of ozone is no longer detectable or noticeable VKM140808 Genlantis Page 6 of 7 11011 Torreyana Road San Diego CA 92121 Toll Free 888 428 0558 U S amp Canada 858 457 1919 www genlantis com 2014 Genlantis and Gene Therapy Systems Inc A N CAUTION Ozone sterilization In progress Do NOT open door until this sign is removed For users with remote control press the remote button to immediately cancel the Ozone cycle and enter the unit into the Scrub cycle Allow the Ozilla device to fully run the Scru
12. rilized with Ozilla MEH Moderate Effect High Materials are noticeably affected by ozone gas and exposure should be limited to short periods of time only Frequent exposure of these materials to ozone gas will require they be discarded or replaced MEL Moderate Effect Low Materials are not affected by infrequent brief exposure but ozone gas can deteriorate materials found under MEL Prolonged exposure to high levels of ozone gas will require they be discarded or replaced ZE Zero Effect materials are not affected by ozone gas sterilize as often as needed Unlisted Materials if a laboratory material is not listed in the table below err on the side of caution and remove these unlisted materials from areas to be sterilized with Ozilla Material Material VKM140808 Genlantis Page 2 of 7 11011 Torreyana Road San Diego CA 92121 Toll Free 888 428 0558 U S amp Canada 858 457 1919 www genlantis com 2014 Genlantis and Gene Therapy Systems Inc Ozilla Ozone Sterilizer Manual Chlorosulfonated polyethylene CSPE Hypalon X Thermoplastic elastomer Hytrel9 Monel Neoprene Polychloroprene Polyamide Polypropylene Glass filled polypropylene GFPP ABS Plastic Acrylic Aluminum Brass Bronze Copper Ethylene propylene diene monomer EPDM Flexelene tubing Low density polyethylene LDPE Poly vinyl chloride PVC Polyacrylate Polyethylene Polysulfide Tygon Butyl Chlorinated polyvinyl chloride CP
13. s in which ozone will contact oxidizable substances See Material Safety Data Sheet for further information 5 IMPORTANT photocopy the CAUTION page in this manual Page 5 or make your own CAUTION note and prepare to affix the note on the cabinet or room door indicating that an ozone sterilization cycle is in progress to avoid disrupting a cleaning cycle and to minimize others exposure to ozone gas Operating the Unit HOURS MINUTES SET TIME In General the TIME SELECT button will cycle through the time digits from left to right two lef amp ost digits are for hours and two righ ost digits are for minutes the SET TIME buttons up and down arrows will increase or decrease the selected blinking digit The Ozilla Sterilizer can be set to operate from a minimum of 1 minute to a maximum 99 hours and 99 minutes for each ozone or de ozonation scrub cycles set to run simultaneously We highly recommend that users keep a log sheet to track users and hours of Ozilla usage this log sheet will also be very useful to guide users in replacing the Scrub Filter it in a timely manner every 3 000 hours or 9 months whichever comes first To set the run time for the ozone cycle For recommended sterilization times based on size of area see Table 1 below a Press the OZONE TIME button the digital display will light up and read either 0000 or a previously set time b Press TIME SELECT button the left most digit wi
14. tte tips gloves safety eyewear lab coats plates small instruments cell culture plasticware and much more The Ozilla Sterilizer generates and releases triatomic oxygen O3 gas which readily gives up a single oxygen atom This single oxygen is able to combine with other molecules odors toxic gases bacteria spores etc and neutralize them via the powerful and natural oxidative process Best of all the Ozilla Sterilizer contains a de ozonation aka scrubbing feature which converts ozone gas back to oxygen at the end of a sterilization cycle hence removing ozone gas from the environment The Ozilla Sterilizer offers the following features and benefits Benefits Sterilizes surfaces and air spaces in multiple areas and locations Eliminates need for harmful liquids chemicals UV rays or damaging heat Powerful reduction or elimination of microscopic organisms Sturdy stainless steel construction Programmable timed operation Simple and easy maintenance Powerful 2000 mgph ozone output De ozonation at 240 cfm Compact portable and lightweight Keeps equipment tools and work areas clean without chemical residue or damage Highly cost effective Safe and efficient Disclaimer The Genlantis Ozilla Ozone Sterilizer is not a replacement or substitute for autoclaves and should not be used to sterilize equipment for use in medical or surgical applications of any kind The Ozilla sterilizing cycles can eliminate most surface contam
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