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Agilent 1260 Infinity Variable Wavelength Detector

1. Column compartment Autosampler a EN S F G a i I ais Thermostat for the ALS optional Solvent cabinet Degasser optional Pump Figure 10 Recommended Two Stack Configuration for 1260 Front View Agilent 1260 Infinity Variable Wavelength Detector User Manual 53 3 Installing the Detector LAN to control software CAN Bus cable to Instant Pilot Thermo cable optional Remote cable AC Power CAN Bus cable AC Power Figure 11 Recommended Two Stack Configuration for 1260 Rear View 54 Agilent 1260 Infinity Variable Wavelength Detector User Manual Installing the Detector3 Installing the Detector Preparations Locate bench space Provi
2. Error Messages In the event of an electronic mechanical or hydraulic failure the detector generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components Wavelength Verification Recalibration Wavelength recalibration is recommended after repair of internal components and on a regular basis to ensure correct operation of the detector The detector uses the deuterium alpha and beta emission lines for wavelength calibration Diagnostic Signals The detector has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems Agilent 1260 Infinity Variable Wavelength Detector User Manual Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the detector The lower left indicates the power supply status the upper right indicates the detector status Status indicator green yellow red Bac Line power switch with green light Figure 33 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is O
3. SEES l athe Agilent Technologies Notices Agilent Technologies Inc 2006 2010 No part of this manual may be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61314 90011 Edition Edition 06 2010 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or
4. 3 Installing the Detector Flow Connections to the Detector Preparations Detector is installed in the LC system Parts required Other modules Parts from accessory kit see Accessory Kit Contents on page 49 Two wrenches 1 45 16 inch for capillary connections Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice The amount of substances should be reduced to the minimal volume required for the analysis Do not operate the instrument in an explosive atmosphere 58 The flow cell is shipped with a filling of isopropanol also recommended when the instrument and or flow cell is shipped to another location This is to avoid breakage due to subambient conditions Agilent 1260 Infinity Variable Wavelength Detector User Manual Installing the Detector3 1 Press the release buttons and remove the front cover to have access to the flow cell area 2 Remove the metal cover and install the flow cell Tighten the cell screws ey Sinn 3 Assemble the column detector capillary Depending on the flow cell type it may
5. Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Lamp The light source for the UV wavelength range is a deuterium lamp As a result of plasma discharge in a low pressure deuterium gas the lamp emits light over the 190 to 600 nm wavelength range Source Lens Assembly The source lens receives the light from the deuterium lamp and focuses it onto the entrance slit Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a 1 mm slit For replacement and calibration purposes to optimize the alignment a slit with a hole is needed Filter Assembly The filter assembly is electromechanically actuated During wavelength calibrations it moves into the light path The filter assembly has two filters installed and is processor controlled OPEN nothing in light path CUTOFF cut off filter in light path at A gt 370 nm HOLMIUM holmium oxide filter for wavelength check A photo sensor determines the correct position Cutoff filter Holmium oxide filter with cutoff filter Figure 3 Filter Assembly Agilent 1260 Infinity Variable Wavelength Detector User Manual 15 1 16 Introduction to the Variable Wavelength Detector Mirror Assemblies M1 and M2 The instrument contains two spherical mirrors M1 and M2 The beam adjustable is vertically and horizontally Both mirrors are identical Grating Assembly The grating sepa
6. F hour measured over one hour period Our published drift specification refer also to Performance Specifications on page 43 is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Agilent 1260 Infinity Variable Wavelength Detector User Manual Site Requirements and Specifications2 CAUTION Condensation within the module Condensation will damage the system electronics Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation This module is designed to operate in a typical electromagnetic environment i e where RF transmitters such as mobile telephones may not be used in close proximity Agilent 1260 Infinity Variable Wavelength Detector User Manual 41 2 Site Requirements and Specifications Physical Specifications Table 13 Physical Specifications Type Weight Dimensions height x width x depth Specification 11 kg 25 Ibs 140 x 345 x 435 mm 5 5 x 13 5 x 17 inches Comments
7. Warnings and Cautions Module is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the module can lead to personal injuries e g shock hazard when the module cover is opened and the instrument is connected to power Never perform any adjustment maintenance or repair of the module with the top cover removed and with the power cord plugged in The security lever at the power input socket prevents that the module cover is taken off when line power is still connected Never plug the power line back in when cover is removed Sharp metal edges Sharp edged parts of the equipment may cause injuries To prevent personal injury be careful when getting in contact with sharp metal areas Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice The amount of substances should be reduced to the minimal volume required for the analysis Do not operate the instrument in an explosive atmosphere Agilent 1260 Infinity Variable Wavelength Detector User Manual 137 9 Maintenance
8. lt 0 005 gt 0 005 gt 0 01 gt 0 025 gt 0 05 gt 0 10 gt 0 20 gt 0 40 0 12 0 12 0 25 0 5 1 0 2 0 4 0 8 0 13 74 13 74 13 74 13 74 6 87 3 43 1 72 0 86 Values in the User Interface may be rounded Table 21 Peak Width Response Time Data Rate G1314C Peak Width min Response Time sec Data Rate Hz lt 0 00125 gt 0 00125 gt 0 0025 gt 0 005 gt 0 01 gt 0 025 gt 0 05 gt 0 10 gt 0 20 gt 0 40 lt 0 031 0 031 0 062 0 12 0 25 0 5 1 0 2 0 4 0 8 0 55 27 5 13 74 13 74 13 74 13 74 6 87 3 43 1 72 0 86 Values in the User Interface may be rounded Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 Optimizing the Detector Additional theoretical information can be found in chapter How to optimize the detector on page 87 Agilent 1260 Infinity Variable Wavelength Detector User Manual 85 4 Using the Detector 86 Agilent 1260 Infinity Variable Wavelength Detector User Manual 87 Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 5 How to optimize the detector Optimizing the Detector Performance 88 This chapter gives hints on how to select the detector parameters and the flow cell rf Agilent Technologies 5 How to optimize the detector Optimizing the Detector Performance The detector has a variety of parameters that can be used to optimize performance The informati
9. Defective power supply Suggested actions Ensure the lamp is connected Replace the top foam and turn on the lamp Exchange the VWM board Exchange the lamp Exchange the power supply Agilent 1260 Infinity Variable Wavelength Detector User Manual Calibration Failed Error Information 7 The intensity maximum was not found during wavelength calibration Calibration 0 Failed Zero order calibration failed Calibration 1 Failed 656 nm calibration failed During zero order and 656 nm calibration the detector searches for the intensity maximum If the maximum is not detected within the scan range the error message is generated Probable cause 1 2 Agilent 1260 Infinity Variable Wavelength Detector User Manual Lamp is OFF Incorrect flow cell installation Flow cell contamination or air bubbles Intensity too low Current step value too far from maximum Misaligned defective grating assembly ProbableCause Suggested actions Switch on the lamp Ensure the flow cell are installed correctly Clean replace flow cell windows or remove air bubbles Replace lamp Enter a different calibration step value different scan range Repeat the calibration Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit Exchange the VWM board 109 7 110 Error Information Holmium Oxide Test Failed The holmium oxide test in the detec
10. Detector Error Messages 107 Lamp Current Missing 107 Lamp Voltage Missing 107 Agilent 1260 Infinity Variable Wavelength Detector User Manual Lamp Ignition Failed 108 Heater Current Missing 108 Calibration Failed 109 Holmium Oxide Test Failed 110 Grating Filter Motor Test Failed 110 Wavelength Check Failed 111 Filter Check Failed 111 8 Test Functions Available Tests versus Interfaces 114 Intensity Test 115 Checking the Photocurrent 117 Wavelength Verification Calibration 118 ASTM Drift and Noise Test 122 Quick Noise Test 123 Dark Current Test 124 Holmium Oxide Test 126 D A Converter DAC Test 128 Grating and Filter Motor Test 131 Test Chromatogram 133 Procedure Using the Agilent LabAdvisor 133 9 Maintenance and Repair Introduction to Maintenance 136 Warnings and Cautions 137 Agilent 1260 Infinity Variable Wavelength Detector User Manual 10 Maintenance Overview of Maintenance 140 Cleaning the Module 141 ExchangingaLamp 142 Exchanging a Flow Cell 145 Repairing the Flow Cells 148 Using the Cuvette Holder 151 Correcting Leaks 154 Replacing Leak Handling System Parts 155 Replacing the Interface Board 156 Replacing the Detector s Firmware 157 11 Parts and Materials for Maintenance Overview of Maintenance Parts 160 Standard Flow Cell 161 Micro Flow Cell 163 Semi micro Flow Cell 164 High Pressure Flow Cell 166 Cuvette Holder 167 Leak Parts 168 Accessory Kit 169 Agilent 1260 Infinity Variable Wavelength Detector User M
11. Therefore flow cells with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in signal to noise ratio For example in Figure 32 on page 90 the noise increased by less than 10 but a 70 increase in signal intensity was achieved by increasing the path length from 6 mm to 10 mm When increasing the path length the cell volume usually increases in our example from 5 13 uL Typically this causes more peak dispersion As Figure 32 on page 90 demonstrates this did not affect the resolution in the gradient separation in our example As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 Agilent 1260 Infinity Variable Wavelength Detector User Manual 89 5 How to optimize the detector Absorbance Analysis of pesticide standard 6 mm optical path length 10 mm optical path length Time min Figure 32 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the Agilent 1260 Infinity VWD it is necessary to have more precise information on path lengths of the VWD flow cells The correct response is expected respo
12. oe 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin Pin Signal name Active HP 1050 Agilent module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 177 Agilent 1260 Infinity Variable Wavelength Detector User Manual 12 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Pin Pin Signal name Active Universal Agilent module TTL 1 White Digital ground o 2 Brown Prepare run Low A 1 ai 3 Gray Start Low DE KEY __ eon 4 Blue Shut down Low ac one 5 Pink Not connected o0 D5 6 Yellow Power on High oo lof 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 178 Agilent 1260 Infinity Variable Wavelength Detector User Manual BCD Cables Identifying Cables 12 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1260 Infinity modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Signal name BCD Digit Agilent module Green
13. set to either 100 mV or 1 V full scale see also Analog Output Settings on page 81 Lamp Type can be set to either G1314 60100 standard VWD lamp or 2140 0590 DAD lamps see also Exchanging a Lamp on page 142 Automatic Turn On lamps can be programmed detector must be on for this Help online help 77 4 Using the Detector Online Spectra 1 To view the online spectra select Online Spectra NOTE This online spectrum is taken during a stop flow condition only while the peak is kept in the flow cell see Scanning with the VWD on page 79 2 Not Ready Information J j ed J A IE Figure 26 Online Spectra Window 2 Change the absorbance and wavelength range according your needs 78 Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 Scanning with the VWD Access to the scan feature is only possible during run 1 Set up a run Start a run 3 While running on the baseline select from the menu Instrument More VWD Blank Scan see Figure 27 on page 79 A background scan is stored in the memory Op Pump Set up Injector Navigation Bar Set up Column Thermostat Set up WD Signal EK C CHEM3 M ore Puma B AFCDE More Injector b Stop a BATCH More Column Thermostat gt S DEF L gt eae Step 1 Blank Scan scan of the background ii panor solvent is stored in the me
14. Balance would do a balance on the detector Figure 22 Online Plot Window 13 If both baselines are stable set the Y range for the detector signal to 100 mAU If you start with a new UV lamp for the first time the lamp may show initial drift for some NOTE time burn in effect 74 Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 14 Select the menu item RunControl gt Sample Info and enter information about this application Figure 23 on page 75 Press OK to leave this screen A G1245C online Method amp Run Control ae strument Method Sequence View Abort Help eme End Run Control DEF_LC M E Sample Info System 2 x Operator Name Wolfgang m Data File Path JE CHEMSTATION 2 DATAS x Subdirectory JISOTEST Manual Filename fiso_01 D le Prefix Counter rm Sample Parameters Location vial 1 blank run if no entry Sample Name fisocratic test sample Sample Amount 0 Multiplier fi ISTD Amount fo Dilution fi Comment Isocratic test sample 1 ul 30 70 H2O0 Acetonitrile 1 5 mljmin H Run Method OK Cancel Help Figure 23 Sample Information 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray position 1 Agilent 1260 Infinity Variable Wavelength Detector User Manual 75 4 Using the Detector Runnin
15. Detector User Manual 117 8 Test Functions Wavelength Verification Calibration Wavelength calibration of the detector is done using the zero order position and 656 nm emission line position of the deuterium lamp The calibration procedure involves two steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector In addition to the zero order and 656 nm alpha emission line calibration the beta emission line at 486 nm and the three holmium lines are used for the complete wavelength calibration process These holmium lines are at 360 8 nm 418 5 nm and 536 4 nm This function is available in e Agilent LabAdvisior preferred e Agilent ChemStation Instant Pilot G4208A WD Wavelength Calibration System 2 xj Calibration history Deviation Zero order Ref lines Time Date 8 8nm O 8nm 21 55 49 07 02 1970 0 0nm Oinm 11 40 47 04 01 2006 i a Deviation HUUU 12nm Zero order 12nm Max Deviation UUM EEEp e 12nm Reference lines 12nm Zero order deviation is 0 0nm Max deviation reference lines 0 0nm Adjust OK Cancel Calibration settings equal to measured one no calibration necessary Figure 36 Wavelength Verification Calibration Agilent Chem
16. G1314 65055 1 Cell screw 1 2 Ring PEEK i 2 O 3 3 Window quartz IA 4 Gasket KAPTON 5 Cell Cover Es Tt 4 2 3 KD SS la 2 NIE NS G lt N Figure 53 High Pressure Flow Cell Agilent 1260 Infinity Variable Wavelength Detector User Manual 165 11 Parts and Materials for Maintenance Cuvette Holder Table 32 Cuvette Holder Item Description Part Number Cuvette Holder G1314 60200 For information the use of the cuvette holder refer to Using the Cuvette Holder on page 151 Figure 54 Cuvette Holder 166 Agilent 1260 Infinity Variable Wavelength Detector User Manual Parts and Materials for Maintenance 11 Leak Parts Table 33 Leak Parts Item Description Part Number 3 Leak funnel 5041 8388 4 Leak funnel holder 5041 8389 5 Clip 5041 8387 6 7 Corrugated tubing 1200 mm lg re order 5 m 5062 2463 Figure 55 Leak Parts Agilent 1260 Infinity Variable Wavelength Detector User Manual 167 11 Parts and Materials for Maintenance Accessory Kit This kit contains some accessories and tools needed for the installation and repair of the detector Table 34 Accessory Kit Parts Description Part Number Accessory kit G1314 68755 Corrugated tubing to waste re order 5 m 5062 2463 Peek outlet capillary kit i d is 0 25 mm PEEK 5062 8535 Fitting male PEEK 2 pk 0100 1516 168 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infini
17. Line voltage Line frequency Power consumption 100 240 VAC 10 50 or 60 Hz 5 220 VA 85 W 290 BTU Wide ranging capability Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL EN 0 55 C 32 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6500 ft Up to 4600 m 14950 ft Installation Category II Pollution Degree 2 Non condensing For storing the instrument For indoor use only 42 Agilent 1260 Infinity Variable Wavelength Detector User Manual Site Requirements and Specifications2 Performance Specifications Table 14 Performance Specifications Type Specification Comments Detection type Light source Wavelength range Short term noise ASTM Drift Linearity Wavelength accuracy Band width Flow cells Control and data evaluation Double beam photometer Deuterium lamp 190 600 nm lt 0 5 x 10 AU at 254 nm 3 x 104 AU hr at 254 nm gt 2 AU 5 upper limit 1nm 6 5 nm typical Standard 14 uL volume 10 mm cell path length and 40 bar 580 psi pressure maximum High pressure 14 uL volume 10 mm cell path length and 400 bar 5800 psi pressure maximum Micro 1 uL volume 5 mm cell path length and 40 bar 580 psi pressure maximum Semi micro 5 uL volume 6 mm cell path length and 40 bar 580 psi
18. Og gt 2COClg 2ZHCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Agilent 1260 Infinity Variable Wavelength Detector User Manual 193 A Appendix e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF 194 Agilent 1260 Infinity Variable Wavelength Detector User Manual Declaration of Conformity for HOX2 Filter We herewith inform you that the used in Agilents absorbance detectors listed in the table below meets the requirements of National Institute of Declaration of Conformity Holmium Oxide Glass Filter Standards and Technology NIST to be applied as certified wavelength standard According to the publication of NIST in J Res Natl Inst Stand Technol 112 303 306 2007 the holmium oxide glass filters are inherently stable with respect to the wavelength scale and need no recertification The expanded uncertainty of the certified wavelength value
19. RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 7 Communication Settings for RS 232C Communication without on board LAN Mode 1 2 3 4 5 6 7 8 Select RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Table 8 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table9 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 10 Parity Settings without on board LAN Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Agilent 1260 Infinity Variable Wavelength Detector User Manual 33 1 Table 11 Introduction to the Variable Wavelength Detector
20. Running the test with Agilent LabAdvisor 1 Run the D A Converter DAC Test for further information see Online Help of user interface Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Test Name DXA Converter Test Description The test switches a test signal to the analog output that can be measured using an integrator or strip chart recorder Module G1314C JP92110261 Approx Time 1 min Status Running an Test Procedure Vv 1 Check Prerequisites ud 2 Switch on Analog Output Converter Test Exi Continue when you are ready to check the Analog Output D C Test with Agilent LabAdvisor Figure 42 2 Observe the analog output signal ADC1 A ADC CHANNEL A 81314A DACODOOD D 76 683 76 555 76 584 76 582 76 53 Fey 0 2 0 4 06 og 1 12 1 4 16 18 mir Figure 43 D A Converter DAC Test Example of Integrator Plot Agilent 1260 Infinity Variable Wavelength Detector User Manual 129 130 Test Functions Running the Test with Instant Pilot The test can be started via the command line 1 To start the test TEST DAC 1 Reply RA 00000 TEST DAC 1 2 To stop the test TEST DAC 0 Reply RA 00000 TEST DAC 0 Test Failed The noise on the step should be less than 3 uV Probable cause Suggested actions 1 Bad cable or grounding problem between Check or replace the cable detector and external device 2 Defective detector main boar
21. Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Boot Resident Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 Swe TEST BOOT 1 1 1 0 0 0 0 0 TEST BOOT 1 1 0 0 1 0 0 0 34 Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 12 Force
22. Therefore intensity spectra will differ slightly between instruments Figure 34 on page 116 shows a typical intensity test spectrum The test is available in e Agilent LabAdvisior preferred e Agilent ChemStation e Instant Pilot G4208A via More Diagnosis VWD Lamp Intensity Test Intensity Test Evaluation The Agilent Lab Advisor and the Instant Pilot evaluate three values automatically and display the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value Agilent 1260 Infinity Variable Wavelength Detector User Manual 115 8 Test Functions Instensity Test with Agilent LabAdvisor Test Name Intensity Test Description The test scans the Intensity spectrum generated by the UV Module G1314C JP92110261 Ee Status Passed Start Time 679 2010 11 44 10 AM Stop Time 6 9 2010 11 44 49 AM m Test Procedure pa y Name Value W 1 Check Prerequisites ACEEEO EiT 177 67 h Y 2 Perform Intensity Test UY Lamp On Time 134h O e a 1028 Coa FETT Cou Highest Intensity 233 087 Counts Intensity Counts 2 3309E 05 2E 05 1 5E 05 1E 05 50000 1028 4 1 1 500 Wavelength nm Figure 34 Intensity Test with Agilent LabAdvisor Intensity Test Failed Probable cause Suggested actions 1 Empty flow cell Ensure the flow cell is filled with water 2 Flow cell windows dirty Repeat the test with the flow cell removed If the
23. and optimizing e troubleshooting and diagnose e maintenance e parts identification e safety and related information Agilent Technologies 2006 2010 Printed in Germany Edition 06 2010 G1314 90011 Apg Agilent Technologies
24. connected 2 Black Analog 3 Red Analog a Sag Agilent 1260 Infinity Variable Wavelength Detector User Manual 175 12 Identifying Cables Remote Cables One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1260 Infinity modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin Pin Signal name Active 3394 Agilent module TTL 9 1 White Digital ground ey NC 2 Brown Prepare run Low 15 3 3 Gray Start Low NC 4 Blue Shut down Low o NC 5 Pink Not connected T NC 6 Yellow Power on High 0 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent module to 3396 Series II 3395A Integrators Use the cable 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 176 Agilent 1260 Infinity Variable Wavelength Detector User Manual Identifying Cables 12 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin Pin Signal name Active 33XX Agilent module TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low 80 P 3 3 Gray Start Low NC 4 Blue Shut down Low A o NC 5 Pink Not connected i ie NC 6 Yellow Power on High
25. for e preparing the system e to learn the set up of an HPLC analysis and e to use it as an instrument check to demonstrate that all modules of the system are correctly installed and connected It is not a test of the instrument performance e Learn about special settings All descriptions are based on the Agilent ChemStation B 02 01 Newer versions may look different Before Using the System Solvent Information Observe recommendations on the use of solvents in chapter Solvents in the pump s reference manual Priming and Purging the System When the solvents have been exchanged or the pumping system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel between the solvent reservoir vacuum degasser when available in the system and the pump Solvents containing volatile ingredients will slightly lose these Therefore priming of the pumping system is required before starting an application Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 Table 17 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation lsopropanol Best solvent to flush air out of the system When switching between reverse lsopropanol phase and normal phase both times After an installation Ethanol or Methanol To clean the system when using buffers Bidistilled water After a solvent change Bidistilled wat
26. gt See T N a Next steps To check for leaks establish a flow and observe the flow cell outside of the cell compartment and all capillary connections Insert the flow cell e Perform Wavelength Verification Calibration on page 118 to check the correct positioning of the flow cell e Replace the front cover Agilent 1260 Infinity Variable Wavelength Detector User Manual 147 10 Maintenance Repairing the Flow Cells When required If the flow cell needs repair due to leaks or contaminations Tools required Wrench 1 4 inch for capillary connections Hexagonal wrench 4 mm Tooth picks Parts required See Standard Flow Cell on page 161 See Micro Flow Cell on page 162 See Semi micro Flow Cell on page 163 See High Pressure Flow Cell on page 165 Preparations Turn off the flow Remove the front cover Remove the flow cell see Exchanging a Flow Cell on page 145 The shown cell parts will differ depending upon the flow cell type For detailed parts schematics refer to above mentioned pages 148 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 1 Cell Screw 2 Conical Springs 3 Ring 1 PEEK 4 Gasket 1 small hole 5 Window Quartz 6 Gasket 2 large hole 7 Cell cover assembly 8 Ring 2 PEEK Figure 46 Standard Flow Cell Disassembling 1 Unscrew the cell screw using a 4 mm hexagonal wrench the Flow Cell 2 Remove
27. limits are still met within the premises Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 190 Agilent 1260 Infinity Variable Wavelength Detector User Manual Appendix A Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test Agilent 1260 Infinity Variable Wavelength Detector User Manual 191 A Appendix UV Radiation 192 Emissions of ultraviolet radiation 200 315 nm from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 36 UV Radiation Limits Exposure day Effective Irradiance 8 hours 0 1 W cm 10 minutes 5 0 W cm Typically the radiation values are much smaller than these limits Table 37 UV Radiation Typical Values Position Effective Irradiance Lamp installed 50 cm dist
28. of the system 66 overview optical path 13 optical system 13 system overview 13 P parameters detector 71 parts and materials for maintenance 159 parts cable 171 analog 174 APG remote 176 BCD 179 CAN 181 external contact 183 overview 172 parts for maintenance 198 accessory kit 169 cuvette holder 167 high pressure flow cell 166 leak panels 168 micro flow cell 163 overview of maintenance parts 160 semimicro flow cell 164 standard flow cell 161 peakwidth settings 72 83 91 performance ecifications 43 optimization 88 photodiode assemblies 16 boards 16 readings 117 photometric accuracy 90 physical specifications 42 humidity 42 line voltage and frequency 42 operating temperature 42 power consumption 42 safety information 42 weight and dimensions 42 power considerations 38 consumption 42 cords 39 preparing the HPLC system 67 radio interference 190 rear of detector 57 recalibration of wavelength 94 remote cables parts 176 repairs introduction 135 overview of simple repairs 140 simple maintenance 136 simple repairs see maintenance 139 replacing firmware 157 response time 84 91 response time versus time constant 45 running the sample 76 S safety class instrument 186 general information 186 lithium batteries 189 symbols 188 sample info 75 scan range step 82 scanning 79 serial number information 19 setting up an analysis 62 s
29. open the Signal Plot information Select the Pump Pressure and the VWD A Signal 254 as signals Change the Y range for the VWD to 1 mAU and the offset to 20 and the pressure offset to 50 The X axis range should be 15 minutes Press OK to exit this screen Method and Run Control n a s SZ estrone foes ag ese oer EMG Sane SIO amp 2 1 0 bar a 028 Ne 5 T om A A A Edit Signal Plot DEFAULT D i Available Signals Selected Signals a uaternary Pump Flow DAD Signal 254 4 Reference 360 PE uaternary Pump AIE Quaternary Pump Pressure RE uaternary Pump B e ace uaternary Pump C 204 uaternary Pump D E data Ha AD B Signal 254 16 Reference 31 E 1 1 GB free o PAD C Signal 210 8 Reference 36 AD D Signal 230 16 Reference 3 x Window DAD A Signal 254 4 Reference 360 100 x axis 15 4 min Type acquired y axis range 1 mau I draw zero line T auto y adjust Offset 20 ye radion Gollecto __ Method Settings D Show Fraction collection ticks T Use method settings Apply to Method OK Figure 21 Edit Signal Plot Window Cancel Help Agilent 1260 Infinity Variable Wavelength Detector User Manual 73 4 Using the Detector The Online Plot Figure 22 on page 74 shows both the pump pressure and the detector absorbance signals Pressing Adjust the signals can be reset to the offset value and
30. page 118 to check the correct positioning of the flow cell e Replace the front cover Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 Using the Cuvette Holder When required If your own standard should be used to checkout the instrument Tools required None Parts required Cuvette Holder G1314 60200 Cuvette with the standard e g NIST certified holmium oxide sample This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications Figure 47 Cuvette Holder Agilent 1260 Infinity Variable Wavelength Detector User Manual 151 10 Maintenance Preparation for this procedure are Remove the normal flow cell e Have cuvette with standard available 1 Locate the cuvette holder on the desk 2 Unscrew the bracket 3 Insert the cuvette with the sample into the holder The clear side of the cuvette must be visible So Light path Clear side Next steps e Turn the lamp ON e Give the lamp more than 10 minutes to warm up e Perform Wavelength Verification Calibration on page 118 to check the correct positioning of the lamp Reset the lamp counter as described in the User Interface documentation 1
31. pressure maximum Agilent ChemStation for LC See Specification Conditions on page 45 See Specification Conditions on page 45 See Specification Conditions on page 45 Self calibration with deuterium lines verification with holmium oxide filter Can be repaired on component level Agilent 1260 Infinity Variable Wavelength Detector User Manual 43 2 Site Requirements and Specifications Table 14 Performance Specifications continued Type Specification Comments Analog outputs Communications Safety and maintenance GLP features Housing Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional Extensive diagnostics error detection and display through Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic records of maintenance and errors Verification of wavelength accuracy with built in holmium oxide filter All materials recyclable 44 Agilent 1260 Infinity Variable Wavelength Detector User Manual Site Requirements and Specifications2 Specification Conditions ASTM Standa
32. the Agilent ChemStation Zorbax Eclipse XDB C18 4 6 x 150 mm 5 um Part No 993967 902 or Part No 5063 6600 Part No 01080 68704 0 15 wt dimethylphthalate 0 15 wt diethylphthalate 0 01 wt biphenyl 0 03 wt o terphenyl in methanol Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 Conditions A single injection of the isocratic test standard is made under the conditions given in Table 19 Table 19 Conditions Flow 1 5 ml minute Stoptime 8 minutes Solvent 100 30 water 70 Acetonitrile Temperature Ambient Wavelength 254 nm Injection Volume 1uL Agilent 1260 Infinity Variable Wavelength Detector User Manual 65 4 Using the Detector Typical Chromatogram A typical chromatogram for this analysis is shown in Figure 14 The exact profile of the chromatogram will depend on the chromatographic conditions Variations in solvent quality column packing standard concentration and column temperature will all have a potential effect on peak retention and response DAD1 A Sig 254 4 Ref 500 100 041014410 140003 D Figure 14 Typical Chromatogram with UV detector Optimization of the System The settings used for this analysis are specific for this purpose For other applications the system can be optimized in various ways Please refer to the section Optimizing the Detector on page 85 66 Agilent 1260 Infinity Variable Wavelengt
33. try a cold start Agilent 1260 Infinity Variable Wavelength Detector User Manual Troubleshooting and Diagnostics 6 Available Tests vs User Interfaces e Depending on the user interface the available tests and the screens reports may vary see Chapter Test Functions and Calibrations e Preferred tool should be the Agilent Diagnostic Software see Agilent Lab Advisor Software on page 156 e Screenshots used within these procedures are based on the Agilent Lab Advisor Software Agilent 1260 Infinity Variable Wavelength Detector User Manual 97 6 Troubleshooting and Diagnostics Agilent LabAdvisor Software 98 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagn
34. 1 BCD 5 20 Violet 2 BCD7 80 Blue 3 BCD 6 40 Yellow 4 BCD 4 10 ken n e a b oa Black 5 BCD 0 1 Z Sy Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Agilent 1260 Infinity Variable Wavelength Detector User Manual 179 12 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin Pin Signal name BCD Digit 3392 3 Agilent module 1 1 BCD 5 20 O 2 2 BCD7 80 a d 3 3 BCD 6 40 T 4 4 BCD 4 10 o 5 5 BCDO 1 1e 6 6 BCD 3 8 cae 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 180 Agilent 1260 Infinity Variable Wavelength Detector User Manual Identifying Cables 12 CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent 1260 Infinity module s CAN bus connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection Agilent 1260 Infinity Variable Wavelength Detector User Manual 181 12 Identifying Cables RS 232 Cable p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cab
35. 52 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 4 Replace the bracket and fix the cuvette Install the cuvette holder in the instrument Next steps e Perform your verification Agilent 1260 Infinity Variable Wavelength Detector User Manual 153 10 Maintenance Correcting Leaks When required If a leakage has occurred in the flow cell area or at the capillary connections Tools required Tissue Two 1 4 inch wrenches for capillary connections Parts required None 1 Remove the front cover 2 Use tissue to dry the leak sensor area Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Waste outlet Leak sensor assembly Figure 48 Drying the Leak Sensor 154 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 Replacing Leak Handling System Parts When required If the parts are corroded or broken Tools required None Parts required Leak funnel 5061 3356 Leak funnel holder 5041 8389 Leak tubing 120 mm 0890 1711 Remove the front cover to have access to the leak handling system Pull the leak funnel out of the leak funnel holder Pull the leak funnel with the tubing out of its location Replace the leak funnel and or the tubing Insert the leak funnel with the tubing in its position Insert the leak funnel into the leak funnel holder uy oO Oo F
36. 600 nm e optional flow cell cartridges standard 10 mm 14 uL high pressure 10 mm 14 uL micro 5 mm 1 uL semi micro 6 mm 5 uL are available and can be used depending on the application needs e easy front access to lamp and flow cell for fast replacement and e built in holmium oxide filter for fast wavelength accuracy verification For specifications Performance Specifications on page 43 Two versions of the Agilent 1260 Infinity Variable Wavelength Detector are available G1314B Agilent 1260 Infinity Variable Wavelength Detector VL standard version G1314C Agilent 1260 Infinity Variable Wavelength Detector VL high data rates for fast HPLC 12 Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Optical System Overview The optical system of the detector is shown in Figure 1 Its radiation source is a deuterium arc discharge lamp for the ultraviolet UV wavelength range from 190 to 600 nm The light beam from the deuterium lamp passes through a lens a filter assembly in position none cut off or holmium oxide an entrance slit a spherical mirror M1 a grating a second spherical mirror M2 a beam splitter and finally through a flow cell to the sample diode The beam through the flow cell is absorbed depending on the solutions in the cell in which UV absorption takes place and the intensity is converted to an electrical signal by means of the sample phot
37. 67 analog output range 77 analog output settings 81 at power on 77 attenuation 72 autobalance 72 automatic turn on 77 data rate 84 199 detector parameters 71 recalibration 94 drift 69 weight 42 EMF 21 enable analysis when lamp is off 82 Z error method 77 isocratic test sample 76 zero offset 72 lamp type 77 load method 70 negative absorbance margins 82 online plot 74 online spectra 78 peakwidth setting 72 peakwidth settings 83 preparing the HPLC system 67 priming and purging the system 62 requirements and conditions 64 response time 84 running the sample 76 sample info 75 scan range step 82 scanning 79 setting up an analysis 62 setup of detector 71 signal plot 73 signal polarity 82 special setpoints 72 82 special settings 77 stable baseline 69 stop and post time 72 stop flow condition 78 the cuvette holder 151 the detector 61 time table 72 turn on 68 typical chromatogram 66 warm up 69 zero offset 72 UV radiation 192 W warm up 69 warnings and cautions 137 wavelength accuracy 43 calibration 118 range 190 600 nm 43 Agilent 1260 Infinity Variable Wavelength Detector Service Manual Index 200 www agilent com In This Book This manual contains the technical reference information about the Agilent 1260 Infinity Variable Wavelength Detector The manual describes the following e introcduction and specifications e installation e using
38. 7 Ring 2 PEEK kit quantity 2 G1314 65066 1 Cell Screw 1 2 Conical Springs 3 Ring 1 PEEK 4 Gasket 1 small hole 5 Window Quartz 6 Gasket 2 large hole 7 Ring 2 PEEK 7 2 6S 1 Figure 50 Standard Flow Cell Agilent 1260 Infinity Variable Wavelength Detector User Manual 161 11 Parts and Materials for Maintenance Micro Flow Cell 1 Cell Screw 2 Conical Springs 3 Ring SST 4 Gasket 1 5 Window Quartz 6 Gasket 2 162 Table 29 Micro Flow Cell Assembly Item Description Part Number Micro flow cell 5 mm 1 pL 40 bar G1314 60081 Capillary column detector SST 400 mm lg 0 12 i d 5021 1823 1 Cell screw 79853 27200 Cell kit micro comprises G1314 65052 two windows two gaskets 1 and two gaskets 2 2 Conical spring kit quantity 10 79853 29100 3 Ring SST kit quantity 2 79853 22500 4 Window quartz kit quantity 2 79853 68742 5 Gasket 1 PTFE quantity 10 79853 68743 6 Gasket 2 PTFE quantity 10 G1314 65053 a1 2 GG 3 4 5 O 6 og gt bate i S OPR Bo O 4 3 0 w o i a Figure 51 Micro Flow Cell Agilent 1260 Infinity Variable Wavelength Detector User Manual Parts and Materials for Maintenance Semi micro Flow Cell Table 30 Semi micro Flow Cell Assembly Item Description Part Number 11 Semi micro flow cell assembly 6 mm 5 pL 40 bar 1 Cell screw Semi micro cell kit consisting of two w
39. 72 Hz G1314B G1314C 0 4 minutes 8 seconds 0 86 Hz G1314B G1314C Values in the User Interface may be rounded 2 Choose the sample wavelength at a longer wavelength than the cut off wavelength of the mobile phase at a wavelength where the analytes have strong absorptivity if you want to get the lowest possible detection limit at a wavelength with moderate absorptivity if you work with high concentrations and preferably where the spectrum is flat for better linearity 3 Consider to use time programming to further optimization Agilent 1260 Infinity Variable Wavelength Detector User Manual 91 5 How to optimize the detector 92 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual ee 6 Troubleshooting and Diagnostics e j e Overview of the Detector s Indicators and Test Functions 94 Status Indicators 95 Available Tests vs User Interfaces 97 Agilent LabAdvisor Software 98 Overview about the troubleshooting and diagnostic features Agy Agilent Technologies 93 6 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 94 Status Indicators The detector is provided with two status indicators which indicate the operational state prerun run and error states of the detector The status indicators provide a quick visual check of the operation of the detector
40. 9 Radio Interference 190 Sound Emission 191 UV Radiation 192 Solvent Information 193 Declaration of Conformity for HOX2 Filter 195 Agilent Technologies on Internet 196 phe Agilent Technologies 185 A Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards 186 This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruptio
41. AN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m Agilent 1260 Infinity Variable Wavelength Detector User Manual 173 12 Identifying Cables Analog Cables 174 One end of these cables provides a BNC connector to be connected to Agilent 1260 Infinity modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin Pin Signal name 3394 6 Agilent module 1 Not connected 2 Shield Analog 3 Center Analog 1 o Agilent Module to BNC Connector p n 8120 1840 Pin Pin Signal name BNC Agilent module Shield Shield Analog Center Center Analog Agilent 1260 Infinity Variable Wavelength Detector User Manual Identifying Cables 12 Agilent Module to General Purpose p n 01046 60105 Pin Pin Signal name 3394 6 Agilent module 1 Not
42. Abort Help Ippes Drives Method M eck chem32 2 E gt methods DEF LC M DEMOCALT M DEMOCAL2M DGALSTST M DGCALAS M DGCALOG1 M DGCALOO2M zi dy DEF_LCM Sequences Gg ed oer cs Method and Run Control SHJ C CHEM32 2 METHODS QI DEMOCAL2 M QI DGALSTST M QI DGCALAS M QI DecaLoqi m QI DGCALOQ2 M QI DGCALOQ3 M QI DGCALOQ4 M QI DGCALOQS M QI DecaLoge m QI DGCALPS M QI DGCHPTST M QO DGCPTEST M QI DGFLDTST M QI DGLEAKT M QI DGNOISE M QI DGNPTEST M QI DGTHMTST M QI pevwopc m QI Isocra m QA ISOPLOT M 70 DEFAULT D Figure 18 no Details Loading Default LC Method Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 9 Click on the module icons Figure 19 and open the Setup of these modules Figure 20 on page 72 shows the detector settings do not change the detector parameters at this time Method and Run Control E n I 2 foe cin S oer EM swe SOO A D DEFAULT D Ke Set up Pump RT Set up YWD Signal EAM a Control Q Data Curves TAU u Fu Online Spectra od SEE B Not Ready Information B Not Ready Information Help E Adata pek E 1 1 GB free po is a ae Figure 19 Open the module menu 10 Enter the pump parameters mentioned under Conditions on pag
43. Agilent LabAdvisor Holmium Oxide Test Failed Probable Causes Suggested Actions 1 Detector not calibrated Recalibrate the detector 2 Dirty or defective flow cell Repeat the test with the flow cell removed If the test is OK exchange the flow cell components 3 Dirty or defective holmium oxide filter Run the holmium oxide filter test If the test fails exchange the filter assembly 4 Optical misalignment Replace the optical unit Agilent 1260 Infinity Variable Wavelength Detector User Manual 127 Test Functions D A Converter DAC Test 128 The detector provides analog output of chromatographic signals for use with integrators chart recorders or data systems The analog signal is converted from the digital format by the digital analog converter DAC The DAC test is used to verify correct operation of the digital analog converter by applying a digital test signal to the DAC The DAC outputs an analog signal of approximately 50 mV if the zero offset of the analog output is set to the default value of 5 which can be plotted on an integrator A continuous square wave with an amplitude of 10 uV anda frequency of approximately 1 cycle 24 seconds is applied to the signal The amplitude of the square wave and the peak to peak noise are used to evaluate the DAC test The test is available in e Agilent LabAdvisior preferred Instant Pilot G4208A When to do the Test e if the detector signal is noisy or missing
44. E QI method caia By senvence acs EME Swart SO T DEFAULT D no Details z E Adata E E 1 1 GB free Figure 16 Turning on the HPLC Module 68 Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 5 Purge the pump For more information see Priming and Purging the System on page 62 6 Allow the detector to warm up of at least 60 minutes to provide a stable baseline see example in Figure 17 NOTE For reproducible chromatography the detector and lamp should be on for at least one hour Otherwise the detector baseline may still drift depending on the environment VND 1 A Wavelength 254 nm LAMP ONWAN_LAMPON2 D mAU 02 0 4 0 6 08 l fi i i i i 10 20 30 4 50 60 min Figure 17 Stabilization of Baseline Agilent 1260 Infinity Variable Wavelength Detector User Manual 69 4 Using the Detector File RunControl Instrui Gal ee 7 For the isocratic pump fill the solvent bottle with the mixture of HPLC grade bi distilled water 80 and acetonitrile 70 For binary and quaternary pumps you can use separate bottles 8 Click on the Load Method button and select DEF_LC M and press OK Alternative double click on the method in the method window The default LC method parameters are transferred into the modules N Eos Lok Name Folders 0K p E FIG M c chem3242 methods C BATCH M a t Method Sequence View
45. Exchange Optical Unit 3 Defective VWM board Exchange the VWM board Filter Motor Test Failed Probable cause Suggested actions 1 Defective filter motor Exchange Optical Unit 2 Defective filter position sensor Exchange Optical Unit 3 Defective VWM board Exchange the VWM board 132 Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Test Chromatogram This function is available from the Agilent Lab Advisor The built in test chromatogram can be used to check the signal path from the detector to the data system or via the analog output to the integrator or data system An example is shown in the figure below The chromatogram is continuously repeated until a stop is executed either by means of a stop time or manually The peak height is always the same but the area and the retention time depend on the set peakwidth see examples below Procedure Using the Agilent LabAdvisor This procedure works for all Agilent 1200 Infinity detectors DAD MWD VWD FLD and RID The example figure is from the RID detector 1 Assure that the default LC method is loaded via the control software 2 Start the Agilent LabAdvisor software B 01 03 SP4 or later and open the detector s Tools selection 3 Open the test chromatogram screen Tools Test Chromatogram Current Status Disabled Switch Test Chromatogram on Switch Test Chromatogram off 4 Turn the Test Chromatogram on 5 Change to the detector s Mod
46. F WD N Replace the front cover Leak funnel holder Leak funnel ie init j EL Leak tubing Waste outlet Leak sensor assembly Figure 49 Replacing Waste Handling System Parts Agilent 1260 Infinity Variable Wavelength Detector User Manual 155 10 Maintenance Replacing the Interface Board When required When defective or for installation of the board or for all repairs inside the detector Part required Interface board BCD G1351 68701 with external contacts and BCD outputs see chapter Hardware Information in the Service Manual LAN Communication Interface board G1369B or G1369 60002 see chapter Hardware Information in the Service Manual Tools required None 1 Install the ESD strap Move the power lock across the 2 If required unscrew and remove the interface board Place power inlet the board on the ESD kit ESD strap 3 If required insert the interface board and fix the screws Next steps e Remove the ESD strap e Reinstall the module into the stack 156 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 Replacing the Detector s Firmware When required The installation of newer firmware might be necessary e if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or e i
47. Instant Pilot G4208A via More Diagnosis VWD Holmium Spectrum Test The test uses the following holmium maxima e 360 8 nm e 418 5 nm e 536 4 nm See also Declaration of Conformity for HOX2 Filter on page 195 When to do the Test e after recalibration e as part of the Operational Qualification Performance Verification procedure or e after flow cell maintenance or repair Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm of the expected value This indicates the detector is calibrated correctly ChemStation revisions below B 01 xx show a limit of 2 nm It should read 1 nm If the test shows a value greater than 1 nm perform a recalibration Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Running the test with Agilent LabAdvisor Test Name Holmium Oxide Test Description The test measures the Holmium spectrum from the built in Holmium filter The spectrum is evaluated for peaks at different Module G1314C JP92110261 TE Status Passed Start Time 6 2 2010 3 40 32 PM Stop Time 6 2 2010 3 43 14 PM m Test Procedure Result E Na ue V1 Check Peresse 47 2 Perform Holmium Oxide Test UVLampOnTime BAH cers Fomu Devatero 350m oom Holmium Deviation to 418 5 nm O00 Holmium Deviation to 536 4 nm O00nm Absorbance AU 5 3754 0 075038 4 n 500 800 Wavelength nm Figure 41 Holmium Test with
48. N Agilent 1260 Infinity Variable Wavelength Detector User Manual 95 6 96 Troubleshooting and Diagnostics Module Status Indicator The detector status indicator indicates one of four possible detector conditions When the status indicator is OFF and power switch light is on the detector is in a prerun condition and is ready to begin an analysis A green status indicator indicates the detector is performing an analysis run mode A yellow indicator indicates a not ready condition The detector is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the detector has detected an internal problem which affects correct operation of the detector Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking modules with on board LAN or yellow blinking modules without on board LAN indicates that the module is in resident mode e g during update of main firmware A fast red blinking modules with on board LAN or fast yellow blinking modules without on board LAN indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or
49. Restore Defaults Scan Range fig0 to 400 nm Step j2 nm m U Cancel Figure 29 Spectra Window Help Margin for negative Absorbance Use this field to modify the detector s signal handling to increase the margin for negative absorbance Use this option if for example your solvent gradient produces a decreasing baseline absorbance and for GPC analyses Limits 100 to 4000 mAU Signal Polarity can be switched to negative if required Enable analysis when lamp is off if the VWD is not used in a dual detector setup lamp off the not ready condition is not stopping the analysis Scan Range Step Used for stop flow scanning see Scanning with the VWD on page 79 Margin for negative Absorbance The higher the value the greater the baseline noise Set this value only if you expect negative absorbance greater than 100 mAU 82 Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 Peakwidth Settings Do not use peak width shorter than necessary see also Set the Detector Parameters on page 91 1 To change the Peakwidth settings select Setup Detector Signals 2 In the section Peakwidth Responsetime click on the drop down list 3 Change the Peakwidth according to your needs Peakwidth enables you to select the peak width response time of Control for your analysis The peak width is defined as the width of a peak fy Online Spectra in m
50. Station 118 Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 The wavelength verification calibration takes about 2 5 minutes and is disabled within the first 10 minutes after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically see Lamp on Routine on page 120 When to Calibrate the Detector The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate e after maintenance flow cell or lamp e after repair of components in the optical unit e after exchange of the optical unit or VWM board e ata regular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and e when chromatographic results indicate the detector may require recalibration Zero Order Calibration The zero order calibration function is used for the electrical calibration of the zero order beam of the monochromator The grating is driven by the stepper motor while the detector searches for the intensity maximum occurring at the zero order position The zero order calibration values are stored in the instrument The deviation is displayed in nm on the user interface see Figure 36 on page 118 656 nm Wavelength Calibration The wavelength ca
51. Wavelength Detector User Manual 105 7 Error Information Fan Failed The cooling fan in the detector has failed The hall sensor on the fan shaft is used by the VWM board to monitor the fan speed If the fan speed falls below two revolutions second for more than five seconds the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Ensure the fan is connected correctly 2 Defective fan Exchange fan 3 Defective main board Exchange the main board Open Cover The top foam has been removed The sensor on the VWM board detects when the top foam is in place If the foam is removed during operation the lamp and grating drive power is switched off and the error message is generated Probable cause Suggested actions 1 Top foam removed Replace the foam 106 Agilent 1260 Infinity Variable Wavelength Detector User Manual Error Information 7 Detector Error Messages These errors are detector specific Lamp Current Missing The lamp anode current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lower current limit the error message is generated Probable cause Suggested actions 1 Lamp disconnected Ensure the lamp connector is seated firmly 2 Top foam removed while lamp is on Replace the top foam and turn ON the lamp 3 Defective or non Agilent lamp Exchange the lamp 4 Defective VWM board Exchange
52. ailable Tests versus Interfaces Depending on the user interface the available tests vary All test descriptions are based on the Agilent LabAdvisor as user interface Table 24 Available Tests versus Interface Interface LabAdvisor ChemStation Instant Pilot Test G4208A Wavelength Verification Re calibration Calibration Tests Maintenance Lamp Intensity Test Tests Tests Diagnosis ASTM Drift and Noise Test Tests n a n a Quick Noise Test Tests n a n a Holmium Test Tests Tests Diagnosis Cell Test Tests Tests n a D A Converter Test Tests Tests n a Dark Current Test Tests Tests n a Filter Grating Motor Test Tests Tests n a Test Chromatogram Tools from command n a line Spectrum Blank Sample Holmium Tools n a n a interface provides passed fail information or a plot 114 Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Intensity Test The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can be used to determine the performance of the lamp and to check for dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics
53. ance average 0 016 W cm Lamp installed 50 cm distance maximum 0 14 W cm Agilent 1260 Infinity Variable Wavelength Detector User Manual Appendix A Solvent Information Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blockage damage of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3
54. and Repair CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components WARNING Eye damage by detector light Eye damage may result from directly viewing the UV light produced by the lamp of the optical system used in this product Always turn the lamp of the optical system off before removing it CAUTION Safety standards for external equipment If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 138 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 10 Maintenance Overview of Maintenance 140 Cleaning the Module 141 ExchangingaLamp 142 Exchanging a Flow Cell 145 Repairing the Flow Cells 148 Using the Cuvette Holder 151 Correcting Leaks 154 Replacing Leak Handling System Parts 155 Replacing the Interface Board 156 Replacing the Detector s Firmware 157 This chapter describes the maintenance of the detector phe Agilent Technologies 139 10 Maintenance Overview of Maintenance The followi
55. anual 12 Identifying Cables Cable Overview 172 Analog Cables 174 Remote Cables 176 BCD Cables 179 CAN LAN Cables 181 RS 232 Cable 182 External Contact Cable 183 A Appendix 10 General Safety Information 186 Safety Standards 186 Safety Symbols 188 Lithium Batteries Information 189 Radio Interference 190 Sound Emission 191 UV Radiation 192 Solvent Information 193 Declaration of Conformity for HOX2 Filter 195 Agilent Technologies on Internet 196 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 1 Introduction to the Variable Wavelength Detector Introduction to the Detector 12 Optical System Overview 13 Flow Cell 14 Electrical Connections 17 Instrument Layout 20 Early Maintenance Feedback EMF 21 Interfaces 23 Setting the 8 bit Configuration Switch 29 This chapter gives an introduction to the detector instrument overview and internal connectors a Agilent Technologies 11 1 Introduction to the Variable Wavelength Detector Introduction to the Detector The Agilent 1260 Infinity Variable Wavelength Detector is designed for highest optical performance GLP compliance and easy maintenance with e data rate up to 13 Hz for standard HPLC with G1314B VWD VL and 55 Hz for fast HPLC with G1314C VWD VL e deuterium lamp for highest intensity and lowest detection limit over a wavelength range of 190 to
56. at it is always possible to access the power plug Remove the power cable from the instrument before opening the cover Do not connect the power cable to the Instrument while the covers are removed WARNING Incorrect line voltage at the module Shock hazard or damage of your instrument can result if the devices are connected to line voltage higher than specified Connect your module to the specified line voltage 38 Agilent 1260 Infinity Variable Wavelength Detector User Manual CAUTION Site Requirements and Specifications2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time Make sure the power connector of the instrument can be easily reached and unplugged Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of the power cords is identical It plugs into the power input socket at the rear of the module The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit Never operate your instrumentation from a power outlet that has no ground connection Ne
57. bable cause Suggested actions 1 The analysis was completed successfully Restart the analysis where required and the timeout function switched off the pump as requested 2 Anot ready condition was present during a Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition period longer than the timeout threshold Agilent 1260 Infinity Variable Wavelength Detector User Manual 101 7 102 Error Information Shut Down An external instrument has generated a shut down signal on the remote line The detector continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Remote Timeout Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in A not ready condi
58. be a PEEK or SST capillary 4 Connect the newly assembled fitting of the capillary to the inlet connector i TEAL Agilent 1260 Infinity Variable Wavelength Detector User Manual 59 3 Installing the Detector 5 Connect the other end of the capillary to the column 6 Connect the PEEK waste capillary to the outlet connector 7 Establish a flow and observe for leakage 8 Replace the front cover The installation of the detector is now complete The detector should be operated with the front cover in place to protect the flow cell area against strong drafts from the outside 60 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 4 Using the Detector Setting up an Analysis 62 Before Using the System 62 Requirements and Conditions 64 Optimization of the System 66 Preparing the HPLC System 67 Running the Sample and Verifying the Results 76 Special Settings of the Detector 77 Control Settings 77 Online Spectra 78 Scanning with the VWD 79 Analog Output Settings 81 Peakwidth Settings 83 Optimizing the Detector 85 This chapter provides information on how to set up the detector for an analysis and explains the basic settings 61 fhe Agilent Technologies 4 Using the Detector Setting up an Analysis 62 This chapter can be used
59. ccessory Kit 168 This chapter provides information on parts for maintenance phe Agilent Technologies 159 11 Parts and Materials for Maintenance Overview of Maintenance Parts Table 27 Maintenance Parts Item Description Part Number Cable CAN assembly 0 5 m 5181 1516 Cable CAN assembly 1 m 5181 1519 Interface board BCD External Contacts G1351 68701 LAN Communication Interface board G1369B or G1369 60002 Instant Pilot G4208A G4208 67001 Deuterium lamp G1314 60100 Standard flow cell 10 mm 14 uL additional flow cell parts see page 161 G1314 60086 Micro flow cell 5 mm 1 pL additional flow cell parts see page 162 G1314 60081 High pressure flow cell 10 mm 14 pL additional flow cell parts see page 165 G1314 60082 Semi micro flow cell 6 mm 5 pL additional flow cell parts see page 163 G1314 60083 Cuvette Holder 61314 60200 Front cover 5067 4691 Leak handling parts see page 167 160 Agilent 1260 Infinity Variable Wavelength Detector User Manual Parts and Materials for Maintenance 11 Standard Flow Cell Table 28 Standard Flow Cell Assembly Item Description Part Number Standard Flow Cell 10 mm 14 uL 40 bar G1314 60086 1 Cell screw kit quantity 2 G1314 65062 2 Conical spring kit quantity 10 79853 29100 3 Ring 1 PEEK kit quantity 2 G1314 65065 4 Gasket 1 small hole KAPTON quantity 10 G1314 65063 5 Window quartz kit quantity 2 79853 68742 6 Gasket 2 large hole KAPTON quantity 10 G1314 65064
60. ctor User Manual 45 2 Site Requirements and Specifications 46 Agilent 1260 Infinity Variable Wavelength Detector User Manual 47 Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 3 Installing the Detector Unpacking the Detector 48 Optimizing the Stack Configuration 50 Installing the Detector 55 Flow Connections to the Detector 58 This chapter provides information on unpacking checking on completeness stack considerations and installation of the module afte Agilent Technologies 3 Installing the Detector Unpacking the Detector CAUTION Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage An Agilent service representative will inspect the instrument at your site and initiate appropriate actions CAUTION Condensation Condensation within the module Condensation will damage the system electronics Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the mod
61. d Exchange the detector main board Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Grating and Filter Motor Test This test moves the grating motor to the end position and displays the motor position step difference This checks the movement of the grating drive mechanism The test is available in e Agilent LabAdvisior preferred e Agilent ChemStation When to do the Test e if recalibration cannot be done successfully or e if the holmium test fails Filter Grating Motor Test with Agilent LabAdvisor Test Name Filter Grating Motor Test Description The test checks the operation of the filter and grating motor Module G1314C JP92110261 ene Status Passed Start Time 6 2 2010 4 32 20 PM Stop Time 6 2 2010 4 32 27 PM ITT TTI TTI TTT TT Test Procedure 1 Check Prerequisites 2 Perform Filter Motor Test 3 Perform Grating Motor Test ANNS 4 Evaluate Data Figure 44 Grating and Filter Motor Test with Agilent LabAdvisor Agilent 1260 Infinity Variable Wavelength Detector User Manual 131 8 Test Functions Filter Grating Motor Test with Instant Pilot The test can be started via the command line To start the test TEST GRAT or TEST FILT Reply RA 00000 TEST GRAT or RA 00000 TEST FILT e Test succeeded when passed Grating Motor Test Failed Probable cause Suggested actions 1 Defective grating motor Exchange Optical Unit 2 Defective grating position sensor
62. d Cold Start Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 SW8 LAN TEST BOOT 1 1 0 0 0 0 0 1 No LAN TEST BOOT 1 1 0 0 1 0 0 1 Agilent 1260 Infinity Variable Wavelength Detector User Manual 35 1 36 Introduction to the Variable Wavelength Detector Agilent 1260 Infinity Variable Wavelength Detector User Manual J e 37 Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 2 Site Requirements and Specifications Site Requirements 38 Physical Specifications 42 Performance Specifications 43 This chapter gives information on environmental requirements physical and performance specifications ae Agilent Technologies 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the module Power Consideration The module power supply has wide ranging capabilities see Table 13 on page 42 It accepts any line voltage in the above mentioned range Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power Make sure th
63. de power connections Unpack the detector Parts required Detector Power cord ChemStation WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power Make sure that it is always possible to access the power plug Remove the power cable from the instrument before opening the cover Do not connect the power cable to the Instrument while the covers are removed 1 Install the LAN interface board in the detector if required see Replacing the Interface Board on page 156 2 Place the detector in the stack or on the bench in a horizontal position 3 Ensure the line power switch at the front of the detector is OFF Agilent 1260 Infinity Variable Wavelength Detector User Manual 55 3 Installing the Detector Status indicator green yellow red Line power switch with green light Figure 12 Front View of Detector The figure above shows the flow cell already installed The flow cell area is closed with a metal cover The flow cell has to be installed as described in Flow Connections to the Detector on page 58 4 Connect the power cable to the power connector at the rear of the detector 5 Connect the CAN cable to other Agilent 1260 Infinity modules 6 Ifa Agilent ChemStation is the controller connect the LAN connection to t
64. dule communication is done via CAN CAN connectors as interface to other modules LAN connector as interface to the control software RS 232C as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector s for signal output Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C on page 32 There is no configuration
65. e 65 Agilent 1260 Infinity Variable Wavelength Detector User Manual 71 4 Using the Detector x m Signal m Time wavelength Stoptime 254 nm as Pump min no Limit E m Peakwidth Responsetime gt O 1 min 2s Timetable Posttime ort 3 min Line Time Wavelength Balance Scan Table Graphic Cancel Help m Analog Output Zero Offset 5 Attenuation 1000 7 mAU r Store additionally T Signal w o Reference J Reference only m Autobalance IV Prerun Tl Postrun Special Setpoints Setup Figure 20 1 signal with individual wavelength setting stop and post time can be set if required peakwidth depends on the peaks in the chromatogram see page 83 time table for programmable actions during the run Zero Offset Limits 1 to 99 in steps of 1 Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale additional signals can be stored with the normal signal for diagnostics autobalance to zero absorbance on the analog output plus offset at begin and or end of run see Special Setpoints on page 82 Detector Settings default 72 Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 11 Pump the water acetonitrile 30 70 mobile phase through the column for 10 minutes for equilibration 12 Click the button and select Change to
66. embly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Early Maintenance Feedback EMF Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counter The detector module provides a EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded The counter can be reset to zero after the lamp is exchanged The detector provides the following EMF counter
67. er After the installation of normal phase Hexane 5 lsopropanol seals P N 0905 1420 Best solvent to flush air out of the system Alternative to lsopropanol second choice if no lsopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties The pump should never be used for priming empty tubings never let the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 ml min 2 Flush all tubes with at least 30 ml of solvent 3 Set flow to required value of your application and close the purge valve Pump for approximately 10 minutes before starting your application Agilent 1260 Infinity Variable Wavelength Detector User Manual 63 4 64 Using the Detector Requirements and Conditions What You Will Need Table 18 lists the items you need to have for the set up of the analysis Some of these are optional not required for the basic system Table 18 What you will need Agilent 1260 Infinity LC system Column Standard Pump plus degassing Autosampler Detector standard flow cell installed Agilent ChemStationor Instant Pilot G4208A optional for basic operation System should be correctly set up for LAN communication with
68. ettings analog output settings 81 peakwidth 83 setup of detector 71 signal plot 73 polarity 82 signals additional diagnostics 72 diagnostic 94 site requirements 38 bench space 40 environment 40 power considerations 38 power cords 39 solvent information 193 sound emission 191 source lens assembly 15 special setpoints 72 82 special settings 77 specification conditions 45 specifications performance 43 physical 42 spectra online 78 stable baseline 69 stack configuration 50 52 rear view 52 standards 42 status indicators 94 96 Agilent 1260 Infinity Variable Wavelength Detector Service Manual Index stop time post time 72 stop flow condition 78 T test functions 94 113 tests 656 nm calibration 119 DAC digital to analog 128 dark current 124 grating amp filter motor 131 holmium oxide 126 intensity of deuterium lamp 115 photocurrent readings 117 test chromatogram 133 wavelength calibration 118 zero order calibration 119 time constant versus response time 45 time table 72 troubleshooting Agilent LC diagnostic software 98 available tests vs interface 114 diagnostic signals 94 error messages 94 100 overview 94 status indicator 96 status indicators 94 95 test functions 94 113 user interfaces 98 turn on automatic 77 U unpacking 48 user interfaces ChemStation Instant Pilot 98 using additional signals diagnostics 72 Agilent ChemStation
69. f anew module with newer firmware is added to a system or if third part control software requires a special version Tools required LAN RS 232 Firmware Update Tool or Agilent Diagnostic Software Instant Pilot G4208A Parts required Firmware tools and documentation from Agilent web site Preparations Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 To load the firmware into the module follow the instructions in the documentation Agilent 1260 Infinity Variable Wavelength Detector User Manual 157 10 Maintenance Module Specific Information Table 26 Module Specific Information G1314B G1314C Initial firmware A 06 02 A 06 02 Compatibility with 1100 1200 series yes all modules should have the firmware from the modules same set Conversion to emulation of G1314A or possible if required G1314B 158 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 11 Parts and Materials for Maintenance Overview of Maintenance Parts 160 Standard Flow Cell 161 Micro Flow Cell 162 Semi micro Flow Cell 163 High Pressure Flow Cell 165 Cuvette Holder 166 Leak Parts 167 A
70. for LAN e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode Figure 6 Location of Configuration Switch example shows a G4212A DAD To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Agilent 1260 Infinity Variable Wavelength Detector User Manual 29 1 Introduction to the Variable Wavelength Detector Table5 8 bit Configuration Switch with on board LAN LAN o b Auto negotiation 10 MBit half duplex 10 MBit full duplex 100 MBit half duplex 100 MBit full duplex Bootp Bootp amp Store Using Stored Using Default TEST h h Boot Resident System Revert to Default Data Coldstart Function SW 3 SW 4 Link Configuration 0 X 1 0 1 0 1 1 1 1 K K x X K K K X System 1 K X SW 5 SW 6 X K SW 7 Init Mode Selection X K SW 8 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 34 30 Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Setting the 8 bit Configuration Switch w
71. g the Sample and Verifying the Results 1 To start a run select the menu item RunControl gt Run Method 2 This will start the modules and the online plot on the Agilent ChemStation will show the resulting chromatogram DAD1 A Sig 254 4 R ef 500 100 04101410 140003 D mAU 11 7 S E a ES S S A a A D A S E S A S S S S O OA OA O EN S E S SER SA a OA EE E Figure 24 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your ChemStation manual supplied with your system 76 Agilent 1260 Infinity Variable Wavelength Detector User Manual Special Settings of the Detector Control Settings Set up VYWD Signal of Control fy Online Spectra 3 Not Ready Information Help Figure 25 WD Control System 2 xj m Lamp Error Method C ion I Take current method sa Analog Output Range Cov go At Power On m Lamp Type J Tum lamp on 61314 60100 m Automatic Turn On J Turn lamp on at Date 29 12 2005 ddimm ppyy Time E 20 03 Dios Cancel Detector control settings Help Agilent 1260 Infinity Variable Wavelength Detector User Manual Using the Detector4 Lamp turn on and off of UV lamp At Power On automatic lamp on at power on Error Method take error method or current method in case of an error Analog Output Range can be
72. gilent 1260 Infinity LC System in the following configuration See Figure 8 on page 51 and Figure 9 on page 52 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required 50 Agilent 1260 Infinity Variable Wavelength Detector User Manual Solvent cabinet Vacuum degasser Local User Interface Autosampler Column compartment Detector Installing the Detector3 Figure 8 Recommended Stack Configuration for 1260 Front View Agilent 1260 Infinity Variable Wavelength Detector User Manual 3 Installing the Detector Remote cable CAN Bus cable to local user interface CAN Bus cable Analog detector signal 1 or 2 outputs per detector AC power LAN to LC ChemStation location depends on detector Figure 9 Recommended Stack Configurationfor 1260 Rear View 52 Agilent 1260 Infinity Variable Wavelength Detector User Manual Installing the Detector3 Two Stack Configuration Two Stack Configuration for Agilent 1260 Infinity LC To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 10 on page 53 and Figure 11 on page 54 Instant Pilot Detector
73. h Detector User Manual Using the Detector4 Preparing the HPLC System 1 Turn on the Agilent ChemStation PC and the monitor 2 Turn on the Agilent 1260 Infinity HPLC modules 3 Start the Agilent ChemStation If the pump autosampler thermostatted column compartment and detector are found the ChemStation screen should look like shown in Figure 15 The System status is red Not Ready System status Method and Run Control LastRun NI 0 oio erica Ri sequence peFuc s EME ea SEO ry P EE Ma ATA O i A A AR Kile ec o rca DEFAULT D E Adata E 1 1 GB free On line plot window details window Figure 15 Initial ChemStation screen Method and Run Control Agilent 1260 Infinity Variable Wavelength Detector User Manual 67 4 Using the Detector 4 Turn on the detector lamp pump and autosampler by clicking the System On button or the buttons below the module icons on the graphical user interface GUI After some time the pump thermostatted column compartment and detector module will turn to green Method and Run Control o NSO Dee oer tc Gag oer oer ucs EME Sart SiO Ka 2 Egeli O a RAR N Ee 0 yan DEFAULT D oF i no Details x 80 r System 2 y System 2 xj System 2 xj 2 j Turn on all pumps 2 Turn on the thermostat 2 J Turn on the Uv lamp of the vWD Module Method and Run Control Last Run
74. he LAN interface board in the detector If an Agilent 1200 Infinity Series DAD MWD ELD is in the system the LAN should be connected to the DAD MWD EFLD due to higher data load 7 Connect the analog cable optional 8 Connect the APG remote cable optional for non Agilent 1260 Infinity instruments 9 Turn ON power by pushing the button at the lower left hand side of the detector The status LED should be green 56 Agilent 1260 Infinity Variable Wavelength Detector User Manual Installing the Detector3 Security lever Interface board 2 N O Analog signals APG remote SS SS SS aaa SG RS 232C OJ SSS CAN Configuration switch Figure 13 Rear View of Detector NOTE The detector is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF some power even if the power switch at the front panel is turned OFF NOTE To disconnect the detector from line unplug the power cord The power supply still uses NOTE The detector was shipped with default configuration settings To change these settings Setting the 8 bit Configuration Switch on page 29 Agilent 1260 Infinity Variable Wavelength Detector User Manual 57
75. he deuterium emission line 656 nm via the reference diode is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable cause Suggested actions 1 Calibration incorrect Recalibrate the detector Filter Check Failed The automatic filter check after lamp ignition has failed When the lamp is switched on the detector moves the cutoff filter into the light path If the filter is functioning correctly a decrease in lamp intensity is seen If the expected intensity decrease is not detected the error message is generated Probable cause Suggested actions 1 Filter motor defective Run the filter motor test to determine if the motor is defective 2 Defective or missing filter Exchange the filter assembly Agilent 1260 Infinity Variable Wavelength Detector User Manual 111 7 Error Information 112 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 8 Test Functions Available Tests versus Interfaces 114 Intensity Test 115 Checking the Photocurrent 117 Wavelength Verification Calibration 118 ASTM Drift and Noise Test 122 Quick Noise Test 123 Dark Current Test 124 Holmium Oxide Test 126 D A Converter DAC Test 128 Grating and Filter Motor Test 131 Test Chromatogram 133 This chapter describes the detector s built in test functions phe Agilent Technologies 113 8 Test Functions Av
76. ift and Noise Test The ASTM Drift and Noise test determines the detector noise over a period of 20 minutes The test is done with HPLC grade water flowing through the flow cell at 1 mL min On completion of the test the noise result is displayed automatically Test Name ASTM Drift and Noise Test Description The test performs ASTM Drift and Noise evaluation without Module G1314C JP92110261 een Status Passed Start Time 6 9 2010 12 13 54 PM Stop Time 6 9 2010 12 34 04 PM m Test Procedure Result Sai Lacs V2 Measure Noise VY a Erue Daa Absorbance mAU 1 2412 1 245 1 25 1 255 1 2598 4 0 2 4 6 10 Time min Figure 38 ASTM Drift and Noise Test Agilent LabAdvisor 122 Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Quick Noise Test The noise test measures the noise of the detector with HPLC grade water flowing through the flow cell at 1 mL min in one minute intervals over a total of 5 minutes The noise of the detector is calculated by using the maximum amplitude for all random variations of the detector signal of frequencies greater than one cycle per hour The noise is determined for 5 one minute intervals and is based on the accumulated peak to peak noise for the intervals At least seven data points per cycles are used in the calculation The cycles in the noise determination are not overlapping In order to obtain reliable results
77. iled 106 general 101 grating filter motor defective 110 heater current missing 108 holmium oxide test failed 110 ignition without cover 106 lamp current missing 107 lamp ignition failed 108 lamp voltage missing 107 leak 103 leak sensor open 104 leak sensor short 104 remote timeout 102 shutdown 102 synchronization lost 103 timeout 101 wavelength check failed 111 exchange interface board 156 external contact cable parts 183 F features 12 GLP 44 instrument layout 20 safety and maintenace 44 filter motor test 131 firmware updates 157 flow cell 14 choosing 88 correction factors 90 cuvette holder parts 167 high pressure parts 166 micro parts 163 path length 90 semimicro parts 164 standard parts 161 types and data 14 43 front of detector 56 G general error messages 101 GPIB 197 removed in 1260 18 grating assembly 16 motor test 131 holmium oxide declaration of conformity 195 filter 15 test 126 information on cuvette holder 151 on lithium batteries 189 on radio interference 190 on solvents 193 on sound emission 191 on UV radiation 192 installation of flow connections 58 of module 55 instrument layout 20 internet 196 introduction 12 to optical system 13 isocratic test sample 76 L lamp 15 initial drift 74 intensity test 115 turn on routine 120 type 43 77 leak correcting 154 panels parts 168 line power consump
78. indows two 1 standard gaskets one 1 semi micro gasket and one 2 semi micro gasket 2 Conical springs pack of 10 3 Ring SST pack of 2 4 PTFE 1 standard gasket pack of 10 5 Quartz window pack of 2 6 Semi micro 1 gasket PTFE pack of 10 7 Semi micro 2 gasket PTFE pack of 10 Inlet capillary 400 mm long 0 12 mm i d G1314 60083 79853 27200 G1314 65056 79853 29100 79853 22500 79853 68743 79853 68742 G1314 65057 G1314 65058 5021 1823 The semi micro 1 and 2 gaskets items 6 and 7 look very similar Do not mix them up Agilent 1260 Infinity Variable Wavelength Detector User Manual 163 11 Parts and Materials for Maintenance Cell screw 1 Conical springs Ring SST Gasket 1 Quartz window Semi micro gasket 1 Semi micro gasket 2 Noor whnr Figure 52 Semi micro Flow Cell 164 Agilent 1260 Infinity Variable Wavelength Detector User Manual Parts and Materials for Maintenance 11 High Pressure Flow Cell Table 31 High Pressure Flow Cell Assembly Item Description Part Number High pressure flow cell 10 mm 14 pL 400 bar G1314 60082 Capillary column detector SST 380 mm lg 0 17 i d G1315 87311 one side not assembled 1 Cell screw 79853 27200 Cell kit Agilent comprises G1314 65054 two windows two KAPTON gaskets and two PEEK rings 2 Ring PEEK kit quantity 2 79853 68739 3 Window quartz kit quantity 2 79853 68734 4 Gasket kit KAPTON quantity 10
79. instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1260 Infinity Variable Wavelength Detector User Manual 187 A Appendix Safety Symbols Table 35 shows safety symbols used on the instrument and in the manuals Table 35 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect the apparatus against damage gt Indicates dangerous voltages Indicates a protected ground terminal o gt Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product 0 Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product gt A WARNING alerts you to situations that could cause physical injury or death Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situation
80. inutes at half the peak height Set the peak width to the Not Ready Information narrowest expected peak in your chromatogram The peak width Help sets the optimum response time for your detector The peak detector ignores any peaks that are considerably narrower or wider than the peak width setting The response time is the time Signal between 10 and 90 of the output signal in response to an input step function When the All spectrum storage option is selected oe then spectra are acquired continuously depending on the setting nm of the peak width The time specified by the peak width is used as PEREA ANIA A EENE a factor in the acquisition of spectra The acquisition time for one spectrum is slightly less than the peak width divided by 8 see solma Es Table 20 on page 84 lt 0 00125 min lt 0 03s a a page ot gt 0 00125 min 0 03 s Tin gt 0 0025 min 0 06 s Limits When you set the peak width in minutes the gt 0 005 min 0 12 s corresponding response time is set automatically and the gt 0 01 min 0 25 s gt 0 025 min 05s appropriate data rate for signal acquisition is selected as shown in gt 0 05 min 1 Table 20 on page 84 gt O1min 2s Figure 30 Peakwidth Setting Agilent 1260 Infinity Variable Wavelength Detector User Manual 83 4 84 Using the Detector Table 20 Peak Width Response Time Data Rate G1314B Peak Width min Response Time sec Data Rate Hz
81. ithout On Board LAN The 8 bit configuration switch is located at the rear of the module Modules that do not have their own LAN interface e g the TCC can be controlled through the LAN interface of another module and a CAN connection to that module Figure 7 Configuration switch settings depend on configured mode All modules without on board LAN e default is ALL DIPS DOWN best settings Bootp mode for LAN e for boot test modes DIPS 1 2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN The following tables represent the configuration switch settings for the modules without on board LAN only Agilent 1260 Infinity Variable Wavelength Detector User Manual 31 1 32 Introduction to the Variable Wavelength Detector Table 6 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 rsvo sys rsvo Rsvo Fc The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS
82. ity The serial number information on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number Serial Number Information 1200 Series The serial number information on the instrument labels provide the following information ccYwwo00000 Format CC Country of manufacturing DE Germany YWW year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 00000 Serial number Agilent 1260 Infinity Variable Wavelength Detector User Manual 19 1 Introduction to the Variable Wavelength Detector Instrument Layout 20 The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disass
83. le 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 182 Agilent 1260 Infinity Variable Wavelength Detector User Manual Identifying Cables 12 External Contact Cable O o Ta o o o cdo o o ol 9150 o o Off O One end of this cable provides a 15 pin plug to be connected to Agilent 1260 Infinity module s interface board The other end is for general purpose Agilent 1260 Infinity Interface Board to general purposes p n G1103 61611 Color Pin Signal Name Agilent module White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 pop Pa lt Red 8 EXT 4 oe Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected Agilent 1260 Infinity Variable Wavelength Detector User Manual 183 12 Identifying Cables 184 Agilent 1260 Infinity Variable Wavelength Detector User Manual This chapter provides addition information on safety legal and web Agilent 1260 Infinity Series Variable Wavelength Detector User Manual A Appendix General Safety Information 186 Lithium Batteries Information 18
84. libration function is used to calibrate the monochrometer against the 656 nm emission line of the deuterium lamp The detector searches for maximum light intensity in the region of the 656 nm emission line The 656 nm calibration values are stored in the instrument Agilent 1260 Infinity Variable Wavelength Detector User Manual 119 120 Test Functions The deviation is displayed in nm on the user interface see Figure 36 on page 118 Lamp on Routine When the lamp is turned on the following routine is implemented During the ignition phase the grating position is reinitialize by moving the grating position lever into the sensor position After ignition a 1 minute warm up phase should stabilize the lamp Check of wavelength setting at 656 nm emission line must be within 3 nm This measurement is taken at the reference side only to have no influence from flow cell condition If not correct then a message for recalibration is displayed Intensity check at 250 nm without cutoff filter value 1 at 250 nm with cutoff filter value 2 The result should be value 2 lt value 1 16 If not OK the cutoff filter does not work correctly Restore the set wavelength position The detector should now be ready condition Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions Wavelength Verification Calibration with Agilent LabAdvisor Test Name Wavelength Calibration Description This procedu
85. mory ET INSTPE Snapshot B LOADT System On ROBUS System Off Sample Scan Blank Scan Step 2 Sample Scan scan of the peak of interest is taken while the peak stays in the Gh Rouri F TE flow cell stop flow condition a a SEQDC Revisions amp Serial s GL seQsu Columns Online Spectrum Sample Scan minus REL STATIS Blank Scan Configure 1100 1200 Access Figure 27 Taking Online Spectra 4 When the peak of interest enters the flow cell stop the flow set flow rate to zero or open the purge valve and wait a few moments to stabilize the concentration Turning off the pump would stop the run and no access to the sample scan is possible Agilent 1260 Infinity Variable Wavelength Detector User Manual 79 4 Using the Detector 5 Select from the menu Instrument More VWD Sample Scan A sample scan is taken in the range defined under Special Setpoints on page 82 and the Online Spectra window see Online Spectra on page 78 displays the result Sample Scan minus Blank Scan 80 Agilent 1260 Infinity Variable Wavelength Detector User Manual Analog Output Settings Using the Detector4 1 To change the Output Range of the analog outputs select VWD Control 2 To change the offset and the attenuation select VWD Signal More Set up VWD Signal of Control fy Online Spectra B Not Ready Information Help WD Control System 2 x z Lamp E
86. n of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Agilent 1260 Infinity Variable Wavelength Detector User Manual Appendix A Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the
87. nector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Agilent 1260 Infinity Variable Wavelength Detector User Manual 27 1 Introduction to the Variable Wavelength Detector Table4 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Re
88. ng pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Table 25 Simple Repairs Procedures Typical Frequency Notes Deuterium lamp If noise and or drift exceeds your application limits or A VWD test should be performed after exchange lamp does not ignite replacement Flow cell exchange If application requires a different flow cell type A VWD test should be performed after replacement Cleaning flow cell parts If leaking or if intensity drops due to contaminated flow A pressure tightness test should be cleaning or exchange cell windows done after repair Leak sensor drying If leak has occurred Check for leaks Leak handling system If broken or corroded Check for leaks replacement 140 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 Cleaning the Module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth allowing liquid to drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module Do not use an excessively damp cloth during cleaning Drain all solvent lines before opening any fittings Agilent 1260 Infinity Variable Wavelength Detector User Manual 141 10 Main
89. nother module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak A leak was detected in the detector The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the VWM board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Leaking flow cell Exchange flow cell components Agilent 1260 Infinity Variable Wavelength Detector User Manual 103 7 Error Information Leak Sensor Open The leak sensor in the detector has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the VWM Ensure the leak sensor is connected correctly board 2 Defective leak sensor Exchange the leak sensor Leak Sensor Short The leak sensor in the detector has failed short circuit The current through the leak sensor is dependent on temperat
90. nse correction factor Please find below the details of the Agilent 1260 Infinity VWD flow cells Table 22 Correction factors for Agilent 1260 Infinity VWD flow cells Flow cell type Cell Partnumber Path length Path length Correction volume nominal actual factor Standard flow cell 14 uL G1314 60086 10mm 10 15 0 19mm 10 10 15 Semi micro flow cell 5 uL G1314 60083 6mm 6 10 0 19mm_ 6 6 10 Micro flow cell T pl G1314 60081 5mm 480 0 19mm 5 48 High Pressure flow cell 14 uL G1314 60082 10mm 10 00 0 19mm 6 5 75 90 However you have to be aware that there are additional tolerance of gasket thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance Agilent 1260 Infinity Variable Wavelength Detector User Manual How to optimize the detector5 Set the Detector Parameters 1 Set peakwidth as close as possible to the width at half height of a narrow peak of interest Table 23 Peakwidth Settings Peakwidth at half height Rise time 10 90 Data rate Module lt 0 00125 minutes lt 0 031 seconds 54 96 Hz G1314C 0 00125 minutes 0 031 seconds 27 48 Hz G1314C 0 0025 minutes 0 062 seconds 13 74 Hz G1314C 0 005 minutes 0 125 seconds 13 74 Hz G1314B G1314C 0 01 minutes 0 25 seconds 13 74 Hz G1314B G1314C 0 025 minutes 0 50 seconds 13 74 Hz G1314B G1314C 0 05 minutes 1 second 6 87 Hz G1314B G1314C 0 1 minutes 2 seconds 3 43 Hz G1314B G1314C 0 2 minutes 4 seconds 1
91. o not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Agilent 1260 Infinity Variable Wavelength Detector User Manual In This Manual This manual covers the Agilent 1260 Infinity Variable Wavelength Detectors G1314B Agilent 1260 Infinity Variable Wavelength Detector VL G1314C Agilent 1260 Infinity Variable Wavelength Detector VL 1 introduction to the Variable Wavelength Detector This chapter gives an introduction to the detector instrument overview and internal connectors 2 Site Requirements and Specifications This chapter gives information on environmental requirements physical and performance specifications 3 Installing the Detector This chapter provides information on unpacking checking on completeness stack considerations and installation of the module 4 Using the Detector This chapter provides information on how to set up the detector for an analysis and explains the basic settings 5 How to optimize the detector This chapter gives hints on how to select the detector parameters and the flow cell 6 Troubleshooting and Diagnostics This chapter describes the detector s built in diagnostic signals Agilent 1260 Infinity Variable Wavelength Detector User Manual 3 10 11 12 Error Information This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from err
92. o on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 Infinity Variable Wavelength Detector User Manual 17 1 Introduction to the Variable Wavelength Detector Security lever Slot for interface _ board Analog Signals APG Remote Configuration switch Power Figure 4 Rear View of Detector NOTE The GPIB interface has been removed with the introduction of the 1260 Infinity modules 18 Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Serial Number Information Serial Number Information 1260 Infin
93. odiode Part of the light is directed to the reference photodiode by the beam splitter to obtain a reference signal for compensation of intensity fluctuation of the light source A slit in front of the reference photodiode cuts out light of the sample bandwidth Wavelength selection is made by rotating the grating which is driven directly by a stepper motor This configuration allows fast change of the wavelength The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light Deuterium lamp est Entrance slit Lens Sample diode A Flow cell a J Beam splitter Mirror M1 g Mirror M2 Reference diode Figure 1 Optical Path of the Variable Wavelength Detector Agilent 1260 Infinity Variable Wavelength Detector User Manual 13 1 Introduction to the Variable Wavelength Detector Flow Cell A variety of flow cell cartridges can be inserted using the same quick and simple mounting system Figure 2 Table 1 Flow Cell Data Cartridge Type Flow Cell STD Semi micro High Pressure Micro Maximum pressure 40 4 40 4 400 40 40 4 bar MPa Path length 10 conical 6 conical 10 conical 5 mm Volume 14 5 14 1 uL Inlet i d 0 17 0 17 0 17 0 1 mm Inlet length 750 750 750 555 mm Outlet i d 0 25 0 25 0 25 0 25 mm Outlet length 120 120 120 120 mm Materials in contact with SST quartz SST quartz SST quartz SST quartz solvent PTFE PEEK PTFE Kapton PTFE 14
94. on below will guide you on how to get the best detector performance Follow these rules as a start for new applications It gives a rule of thumb for optimizing the detector parameters Match the Flow Cell to the Column Figure 31 recommends the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution Typical peak Recommended flow cell width 0 025 min Semi micro 0 05 min flow cell Standard 0 1 min flow cell 0 2 min Typical flow rate 0 05 0 2 ml min 0 2 0 4 ml min 0 4 0 8 ml min 1 2 ml min Internal column diameter Figure 31 Choosing a Flow Cell 88 Agilent 1260 Infinity Variable Wavelength Detector User Manual How to optimize the detector5 Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance I Absorbance logT g e C d where T is the transmission defined as the quotient of the intensity of the transmitted light divided by the intensity of the incident light Ip is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature and other parameters C mol L is the concentration of the absorbing species and d cm is the path length of the cell used for the measurement
95. onditions 45 3 Installing the Detector Unpacking the Detector 48 Delivery Checklist 49 Detector Accessory Kit Contents 49 Optimizing the Stack Configuration 50 One Stack Configuration 50 Two Stack Configuration 53 Installing the Detector 55 Flow Connections to the Detector 58 4 Using the Detector Setting up an Analysis 62 Before Using the System 62 Requirements and Conditions 64 Optimization of the System 66 Preparing the HPLC System 67 Running the Sample and Verifying the Results 76 Special Settings of the Detector 77 Control Settings 77 Online Spectra 78 Scanning with the VWD 79 Analog Output Settings 81 Special Setpoints 82 Peakwidth Settings 83 Optimizing the Detector 85 Agilent 1260 Infinity Variable Wavelength Detector User Manual 5 How to optimize the detector Optimizing the Detector Performance 88 Match the Flow Cell to the Column 88 Set the Detector Parameters 91 6 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 94 Status Indicators 95 Power Supply Indicator 95 Module Status Indicator 96 Available Tests vs User Interfaces 97 Agilent LabAdvisor Software 98 7 Error Information What Are Error Messages 100 General Error Messages 101 Timeout 101 Shut Down 102 Remote Timeout 102 Synchronization Lost 103 Leak 103 Leak SensorOpen 104 Leak Sensor Short 104 Compensation Sensor Open 105 Compensation Sensor Short 105 Fan Failed 106 Open Cover 106
96. optical unit 2 Defective sample or reference VWA board Exchange optical unit 3 Defective VWM board Exchange the VWM board Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Dark Current Test with Agilent LabAdvisor Test Name Dark Current Test Description The test measures the dark current from the detector optic Module G1314C JP92110261 Status Passed Start Time 6 2 2010 4 03 32 PM Stop Time 6 2 2010 4 07 41 PM Test Procedure Result Nan Valu v 1 Check Prerequisites Maximum Sample Intensity Measured 4 064 Counts W 2 Perform Dark Current Test Maximum Reference Intensity Measured 4 020 Counts Y 3 Switch UV Lamp on again 4 Evaluate Data Intensity Counts 4064 4060 4050 4040 1 1 1 1 1 1 1 1 1 1 0 0 2 0 4 0 6 0 8 1 12 1 4 1 6 1 8 1 9947 Time min Intensity Counts 4020 4010 4000 3996 1 1 1 1 1 1 1 1 1 1 0 0 2 0 4 0 6 0 8 1 1 2 1 4 1 6 1 8 1 9947 Time min Figure 40 Dark Current Test Agilent LabAdvisor Agilent 1260 Infinity Variable Wavelength Detector User Manual 125 Test Functions Holmium Oxide Test 126 This test verifies the calibration of the detector against the three wavelength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima Figure 41 shows a holmium test spectrum The test is available in e Agilent LabAdvisior preferred e
97. or conditions Test Functions This chapter describes the detector s built in test functions Maintenance and Repair This chapter provides general information on maintenance and repair of the detector Maintenance This chapter describes the maintenance of the detector Parts and Materials for Maintenance This chapter provides information on parts for maintenance Identifying Cables This chapter provides information on cables used with the Agilent 1260 Infinity modules Appendix This chapter provides addition information on safety legal and web Agilent 1260 Infinity Variable Wavelength Detector User Manual Contents 1 introduction to the Variable Wavelength Detector Introduction to the Detector 12 Optical System Overview 13 Electrical Connections 17 Serial Number Information 19 Instrument Layout 20 Early Maintenance Feedback EMF 21 EMF Counter 21 Using the EMF Counters 21 Interfaces 23 Overview Interfaces 25 Setting the 8 bit Configuration Switch 29 Setting the 8 bit Configuration Switch On Board LAN 29 Setting the 8 bit Configuration Switch without On Board LAN 31 Communication Settings for RS 232C 32 Special Settings 34 2 Site Requirements and Specifications Site Requirements 38 Power Consideration 38 Power Cords 39 Bench Space 40 Environment 40 Physical Specifications 42 Agilent 1260 Infinity Variable Wavelength Detector User Manual Performance Specifications 43 Specification C
98. ostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual c00 ee Error Information e i e j What Are Error Messages 100 e General Error Messages 101 Detector Error Messages 107 This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions a Agilent Technologies 99 7 Error Information What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument logbook 100 Agilent 1260 Infinity Variable Wavelength Detector User Manual Error Information 7 General Error Messages General error messages are generic to all Agilent 1200 Infinity Series modules Timeout The timeout threshold was exceeded Pro
99. possible on main boards with on board LAN These are pre configured for 19200 baud 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Agilent 1260 Infinity Variable Wavelength Detector User Manual 25 1 26 Introduction to the Variable Wavelength Detector Table3 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC RX 2 2 RX TX 3 _X 3 TX DTR 4 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS E ne RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 5 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D con
100. quest to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 28 Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Setting the 8 bit Configuration Switch Setting the 8 bit Configuration Switch On Board LAN The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e g G1315 65C D G1314D E F G4212A B G4220A e Default is ALL switches DOWN best settings Bootp mode
101. rates the light beam into all its component wavelengths and reflects the light onto mirror 2 Beam Splitter Assembly The beam splitter splits the light beam One part goes directly to the sample diode The other part of the light beam goes to the reference diode Photo Diodes Assemblies Two photo diode assemblies are installed in the optical unit The sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in the front of the optical unit Photo Diode ADC analog to digital converter The photo diode current is directly converted to 20 bit digital data direct photo current digitalization The data is transferred to the detector main board VWM The photo diode ADC boards are located close to the photo diodes Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Electrical Connections e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems e The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and s
102. rd Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Standard flow cell path length 10 mm flow 1 ml min LC grade methanol Noise Noise lt 0 5 x 10 5 AU at 254 nm TC 2 s ASTM RT 2 2 TC Linearity Linearity is measured with caffeine at 265 nm The specifications are based on the standard lamp G1314 60100 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit gt two hours ASTM measurements require that the detector should be turned on at least 24 hours before start of testing Time Constant versus Response Time According to ASTM E1657 98 Standard Practice of Testing Variable Wavelength Photometric Detectors Used in Liquid Chromatography the time constant is converted to response time by multiplying by the factor 2 2 Agilent 1260 Infinity Variable Wavelength Dete
103. re performs a Wavelength Verification and Recalibration Module G1314C JP92110261 Approx Time 3 min Status Running Test Procedure m Result r Name Value YW 1 Check Prerequisites Accumulated UV Lamp Burn Time 3241h Y 2 Wavelength Verification UV Lamp On Time i 3 Calibrate Detector Time to Wait Before Wavelength Calibration 0 00 min dx Wavelength Calibration x bx Do you want to calibrate the detector using the wavelength verification results Wavelength Gap of previous O order Calibra Wavelength Gap of O order Calibration 0 400 nm Homa wesen tas tose aor Yy 2 Wavelength Verification UY Lamp On Time Y 3 Calibrate Detector Test Name Wavelength Calibration Description This procedure performs a Wavelength Verification and Module G1314CJP92110261 Sane Status Passed Start Time 6 2 2010 3 12 43 PM Stop Time 6 2 2010 3 21 33 PM AARMANNITNAANANANMI m Test Procedure m Result Name Value y 1 Check Prerequisites Accumulated UY Lamp Burn Time Time to Wait Before Wavelength Calibration Maximum Wavelength Gap of previous Calib 0 200 nm Wavelength Gap of O order Calibration 0 400 nm Agilent 1260 Infinity Variable Wavelength Detector User Manual Maximum Wavelength Gap of Calibration Calibrate Detector with Wavelength Verificati Figure 37 Wavelength Verification and Calibration Agilent LabAdvisor 121 8 Test Functions ASTM Dr
104. rror Method I Take current method C off T Tum lamp on r Analog Output Range Cow 1V At Power On gt Lamp Type a131 4 60100 m Automatic Turn On I Tum lamp on at Date 29 12 2005 Time 11 20 03 lt q d d MM Yyyy gt lt hhammiss gt OK Cancel Help Set up VWD Signal gt of Control fy Online Spectra D Not Ready Information Help Figure 28 x m Signal Time Analog Output Wavelength Stoptime Zero Offset as Pump 4 mi 254 nm aera min 5 Peakwidth Responsetime Posttime Attenuation gt O1min 2s ad ot gt min Analog Output Range can be set to either 100 mV or 1 V full scale Zero Offset can be set to either 100 mV or 1 V full scale Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale mAU 1000 Analog Output Settings 3 Change the values if required Agilent 1260 Infinity Variable Wavelength Detector User Manual 81 4 Using the Detector Special Setpoints 1 To change the offset and the attenuation select VWD Signal More Special Setpoints at up vWD Signal E gt vWD Special Setpoints System 2 x of Control fu Online Spectra Margin for negative Absorbance piam 8 Mot Ready Information Help Signal Polarity Positive Negative Tl Enable analysis when lamp is off
105. s e Deuterium Lamp On Time Using the EMF Counters The user settable EMF limits for the EMF counter enables the early maintenance feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analysis high or low sensitivity analysis wavelength and so on therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Agilent 1260 Infinity Variable Wavelength Detector User Manual 21 1 22 Introduction to the Variable Wavelength Detector Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary take note of the values displayed by lamp counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1260 Infinity Variable Wavelength Detector User Manual Introduction to the Variable Wavelength Detector 1 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote P
106. s is 0 2 om Agilent Technologies guarantees as required by NIST that the material of the filters is holmium oxide glass representing the inherently existent holmium oxide absorption bands Test wavelengths Product Number 79883A 1090 79854A G1306A G1315A G1365A 1050 1050 1100 G1315B C G1365B C G1600A G7100A 1100 1200 1260 Measured Wavelength Accuracy Wavelength 79853C G1314A B C G1314DEF 1100 1200 1260 G4286 A B C 1120 1220 The variation in Measured Wavelength depends on the different Optical Bandwidth P N 89550 90501 May 19 2010 Revision H Effective by May 19 2010 Agilent 1260 Infinity Variable Wavelength Detector User Manual te Agilent Technologies Appendix A 195 A Appendix Agilent Technologies on Internet 196 For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1260 Infinity modules for download Agilent 1260 Infinity Variable Wavelength Detector User Manual A absorbance Beer Lambert 89 accessory kit parts 169 Agilent ChemStation 67 LC diagnostic software 98 on internet 196 Agilent LabAdivsor Software 98 algea information 193 analog cables parts 174 output range 77 81 output settings 81 ASTM environmen
107. s that could cause loss of data or damage of equipment Do not proceed beyond a caution until you have fully understood and met the indicated conditions 188 Agilent 1260 Infinity Variable Wavelength Detector User Manual Appendix A Lithium Batteries Information Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries Lithium batteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1260 Infinity Variable Wavelength Detector User Manual 189 A Appendix Radio Interference If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference
108. software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend Software and technical data rights granted to federal government customers include only those rights customarily provided to end user Customers of Software Agilent provides this customary commercial license in Software and technical data pursuant to FAR 12 211 Technical Data and FAR 12 212 Computer Software and for Department of Defense purchases DFARS 252 227 7015 Technical Data Commercial Items and DFARS 227 7202 3 Rights in Commercial Computer Software or Computer Software Documentation If a federal government or other public sector Customer has a need for rights not conveyed under these terms it must negotiate with Agilent to establish acceptable terms in a written agreement executed by all relevant parties Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death D
109. tal conditions 40 attenuation 72 autobalance 72 automatic turn on 77 band width 6 5 nm 43 battery safety information 189 BCD cables parts 179 beam splitter 16 Beer Lambert law 89 bench space 40 boards photodiode boards ADC 16 C cable connecting APG remote 52 connecting CAN 52 connecting GPIB 52 connecting LAN 52 connecting the analog 56 connecting the APG remote 56 connecting the CAN 56 connecting the ChemStation 52 connecting the LAN 56 connecting the power 52 56 overview and identification 172 calibration 656 nm 119 zero order 119 CAN cables parts 181 connecting interface 56 cautions and warnings 137 chromatogram 66 configuration stack 50 connector locations at rear 57 correction factors for flow cells 90 cutoff filter 15 cuvette holder 151 D DAC test 128 dark current test 124 data rate 84 91 declaration of conformity 195 delivery checklist 49 detection type 43 detector error messages 107 diagnostic signals 94 test functions 113 dimensions 42 drift 43 69 initial 74 E electrical connections descriptions of 17 EMF early maintenance feedback 21 enable analysis when lamp is off 82 entrance slit assembly 15 environment 40 error messages 94 100 calibration failed 109 compensation sensor open 105 compensation sensor short 105 detector 107 Agilent 1260 Infinity Variable Wavelength Detector Service Manual Index fan fa
110. tenance Exchanging a Lamp When required If noise or drift exceeds application limits or lamp does not ignite Tools required Screwdriver POZI 1 PT3 Parts required Deuterium lamp G1314 60100 If you want to use the Agilent DAD lamp instead of the VWD lamp you have to change the lamp settings in the VWD Configuration to lamp type 2740 0590 This ensures that the DAD lamp s filament heating is operated like in the DAD The instrument specifications are based on the VWD lamp WARNING Injury by touching hot lamp If the detector has been in use the lamp may be hot If so wait for lamp to cool down 142 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 Preparations for this procedure 1 Press the release buttons and remove the front cover to have access to the lamp area Turn the lamp OFF 8 Unscrew the lamp cover and remove it 9 Unscrew disconnect and replace the lamp Insert fix and reconnect the lamp l T g o lt Agilent 1260 Infinity Variable Wavelength Detector User Manual 143 10 Maintenance 10 Replace the lamp cover 11 Replace the front cover Next steps Reset the lamp counter as described in the User Interface documentation e Turn the lamp ON Give
111. test passes exchange the flow cell windows 3 Optics defect Clean replace optical components 4 Defective lamp or optics Exchange the lamp 116 Agilent 1260 Infinity Variable Wavelength Detector User Manual Test Functions 8 Checking the Photocurrent To identify problems with the flow cell contaminated windows air bubbles and so on you can use raw data from the sample and reference photodiode unfiltered and not logarithmic The photocurrent information RAW SAMPLE REFERENCE is available in e Agilent Instant Pilot G4208A via More Diagnosis VWD LampIntensity Test re Diagnosis Intensity VWD SL oN At 254 nm sample cts measured 4018723 JAt 254 nm reference cts measured 3612108 Done Min intens gt 6 400 cts measured 25507 Passed Avrg intens gt 160 000 cts measured 884724 Passed Finished Passed Y Done Max intens gt 320 000 cts measured 6039917 _ _ Passed bd Exit Used to determine the performance of the lamps and optics ky 15 48 Figure 35 Photocurrent readings with Instant Pilot Photocurrent Evaluation With a clean cell the counts for sample and reference are in the same range If like in this example the sample side shows much less the flow cell might have a problem due to e dirty windows e absorbing solvents compounds e not correctly mounted flow cell or e air bubbles Agilent 1260 Infinity Variable Wavelength
112. the SST rings using a pair of tweezers 3 Use adhesive tape to remove the peek ring the window and the gasket Do not use tweezers to remove windows as the surfaces can easily be scratched CAUTION y 4 Repeat step 1 through step 3 for the other window keep the parts separate otherwise they could be mixed Cleaning the Flow 5 Pour isopropanol into the cell hole and wipe clean with a piece of lint free Cell Parts cloth 6 Clean the windows with ethanol or methanol Dry it with a piece of lint free cloth Always use new gaskets Agilent 1260 Infinity Variable Wavelength Detector User Manual 149 10 Maintenance Reassembling the 150 Flow Cell 7 Hold the flow cell cassette horizontally and place gasket in position Ensure both cell holes can be seen through the holes of gasket The semi micro 1 and 2 gaskets items 6 and 7 Semi micro Flow Cell on page 164 look very similar Do not mix them up 8 Place the window on gasket 9 Place the peek ring on the window 10 Insert the conical springs Make sure the conical springs point towards the window Otherwise tightening the cell screw might break the window 11 Screw the cell screw into the flow cell and tighten the screw 12 Repeat the procedure for the other cell side Next steps e Reconnect the capillaries see Exchanging a Flow Cell on page 145 e Perform a leak test If OK insert the flow cell e Perform Wavelength Verification Calibration on
113. the VWM board 5 Defective power supply Exchange the power supply Lamp Voltage Missing The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operation If the anode voltage falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective or non Agilent lamp Exchange the lamp 2 Defective power supply Exchange the power supply 3 Defective VWM board Exchange the VWM board Agilent 1260 Infinity Variable Wavelength Detector User Manual 107 7 Error Information Lamp Ignition Failed The lamp failed to ignite The processor monitors the lamp current during the ignition cycle If the lamp current does not rise above the lower limit within 2 5 seconds the error message is generated Probable cause 1 2 3 Lamp disconnected Defective or non Agilent lamp Defective power supply 4 Defective VWM board Heater Current Missing Suggested actions Ensure the lamp is connected Exchange the lamp Exchange the power supply Exchange the VWM board The lamp heater current in the detector is missing During lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within one second the error message is generated Probable cause 1 2 108 Lamp disconnected Ignition started without the top foam in place Defective VWM board Defective or non Agilent lamp
114. the lamp more than 10 minutes to warm up Perform Wavelength Verification Calibration on page 118 to check the correct positioning of the lamp 144 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 Exchanging a Flow Cell When required If application needs a different type of flow cell or the flow cell needs repair Tools required Two 1 4 inch wrenches for capillary connections Parts required G1314 60086 10 mm 14 uL 40 bar Micro flow cell 5 mm 1 pL 40 bar G1314 60081 Semimicro flow cell 6 mm 5 uL 40 bar G1314 60083 High pressure flow cell 10 mm 14 pL 400 bar G1314 60082 Preparations for this procedure f Press the release buttons and remove the front cover to have access to the flow cell area Turn the lamp OFF Agilent 1260 Infinity Variable Wavelength Detector User Manual 145 10 Maintenance 2 Disconnect the inlet and outlet capillaries 3 Unscrew the thumb screws parallel and remove the flow cell l ci iih Note Replace the flow cell and fix the thumb screws Reconnect ei the inlet and outlet capillaries to the fl Il If you want to maintain flow cell parts see a eee wert energie Repairing the Flow Cells on page 148 or the information provided with your flow cell 146 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance 10 5 Replace the front cover i Wy
115. the lamp should be turned on for at least 10 minutes prior to measurement Test Name Quick Noise Test Description The test performs a quick Noise Evaluation without reference Module G1314C JP92110261 Status Passed Start Time 6 9 2010 12 40 40 PM Stop Time 6 9 2010 12 45 46 PM m Test Procedure Result 2A 1 Check Prerequisites Vv 2 Measure Noise Y 3 Evaluate Data Name Value Absorbance mau 1 2354 1 238 1 24 1 242 1 244 1 2453 r 0 A 25 Time min Figure 39 Quick Noise Test Agilent LabAdvisor Agilent 1260 Infinity Variable Wavelength Detector User Manual 123 Test Functions Dark Current Test 124 The dark current test measures the leakage current from the sample and reference circuits The test is used to check for defective sample or reference diodes or ADC circuits which may cause non linearity or excessive baseline noise During the test the lamp is switched off Next the leakage current from both diodes is measured When to do the Test e if the detector signal is noisy Interpreting the Results The output signal of the ADC noise corresponds to AD counts with 1 V or 0 1 V full scale setting The ADC noise must be taken over a time of 10 minutes for the results to be accepted Average counts should be less than 7900 counts Dark Current Test Failed Probable Causes Suggested Actions 1 Defective sample or reference diode Exchange
116. tion 42 voltage and frequency 42 linearity 43 45 M maintenance 139 correcting leaks 154 exchanging flow cell 145 exchanging interface board 156 exchanging lamps 142 exchanging leak handling system 155 for parts see parts for maintenance 159 introduction 135 overview 140 replacing firmware 157 simple repairs 136 standard flow cell 148 using the cuvette holder 151 message calibration failed 109 calibration lost 111 compensation sensor open 105 compensation sensor short 105 fan failed 106 grating filter motor defective 110 heater current missing 108 holmium oxide test failed 110 ignition without cover 106 lamp current missing 107 lamp ignition failed 108 lamp voltage missing 107 leak 103 leak sensor open 104 leak sensor short 104 meaning of 100 remote timeout 102 shutdown 102 synchronization lost 103 timeout 101 wavelength check failed 111 method error 77 load 70 mirror assemblies 16 Agilent 1260 Infinity Variable Wavelength Detector Service Manual Index N negative absorbance margins 82 noise 45 noise short term 43 0 online plot 74 online spectra 78 operating temperature 42 optical unit beam splitter assembly 16 entrance slit assembly 15 filter 15 filter assembly 15 flow cell 14 grating assembly 16 lamp 15 mirrors 16 photodiode assemblies 16 photodiode boards 16 source lens assembly 15 optimization detector performance 88
117. tion is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation Agilent 1260 Infinity Variable Wavelength Detector User Manual Error Information 7 Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in a
118. tor has failed During the holmium test the detector moves the holmium filter into the light path and compares the measured absorbance maxima of the holmium oxide filter with expected maxima If the measured maxima are outside the limits the error message is generated Probable cause Suggested actions 1 Misaligned defective grating assembly Ensure the flow cell is inserted correctly and is free from contamination cell windows buffers and so on Run the filter motor test to determine if the grating motor assembly is defective Exchange the filter motor assembly Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit Grating Filter Motor Test Failed The motor test has failed Test 0 Failed Filter motor Test 1 Failed Grating motor During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the error message is generated Probable cause Suggested actions 1 Defective motor assembly Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit Agilent 1260 Infinity Variable Wavelength Detector User Manual Error Information 7 Wavelength Check Failed The automatic wavelength check after lamp ignition has failed When the lamp is switched on the detector waits 1 minute to warm up the lamp Then a check of t
119. ty Series Variable Wavelength Detector User Manual 12 Identifying Cables Cable Overview 172 Analog Cables 174 Remote Cables 176 BCD Cables 179 CAN LAN Cables 181 RS 232 Cable 182 External Contact Cable 183 This chapter provides information on cables used with the Agilent 1260 Infinity modules egy Agilent Technologies 171 12 Identifying Cables Cable Overview 172 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Agilent module to 3396 Series Il 3395A Integrators on page 176 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose Agilent 1260 Infinity Variable Wavelength Detector User Manual Identifying Cables 12 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 C
120. ule If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 48 Agilent 1260 Infinity Variable Wavelength Detector User Manual Installing the Detector3 Delivery Checklist Ensure all parts and materials have been delivered with the module The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 15 Variable Wavelength Detector Checklist Description Quantity Variable wavelength detector 1 Power cable 1 Flow cell As ordered User Manual 1 Accessory kit see Table 16 1 Detector Accessory Kit Contents Detector Accessory Kit Contents p n G1314 68755 Table 16 Accessory Kit Contents Description Part Number Quantity CAN cable 0 5 m 5181 1516 1 PEEK outlet capillary kit 5062 8535 1 Fitting male PEEK 2 pk 0100 1516 1 Tubing assembly 1 2 m 5063 6527 1 Agilent 1260 Infinity Variable Wavelength Detector User Manual 49 3 Installing the Detector Optimizing the Stack Configuration If your detector is part of a complete AgilentLC system you can ensure optimum performance by installing the following configurations These configurations optimizes the system flow path ensuring minimum delay volume One Stack Configuration One Stack Configuration for Agilent 1260 Infinity LC Ensure optimum performance by installing the modules of the A
121. ule Service Center and add the detector signal to the Signal Plot window 6 To start a test chromatogram enter in the command line STRT Agilent 1260 Infinity Variable Wavelength Detector User Manual 133 8 Test Functions 35 Send RA 0000 STRT G13624 DE91600336 5 4 2010 3 19 20 PM E heater temperature RA 0000 STOP G13624 DE91600336 5 4 2010 3 24 11 PM stor 7 Send Signal configuration Available signals Signal A m4 G13 7 Add Select signal Signal A PRIU G13 z Remove IY Autoscroll Generic plot E command line Time min Figure 45 Test Chromatogram with Agilent LabAdvisor 7 To stop the test chromatogram enter in the command line STOP NOTE The test chromatogram is switched off automatically at the end of a run 134 Agilent 1260 Infinity Variable Wavelength Detector User Manual Agilent 1260 Infinity Series Variable Wavelength Detector User Manual 9 Maintenance and Repair Introduction to Maintenance 136 Warnings and Cautions 137 This chapter provides general information on maintenance and repair of the detector afte Agilent Technologies 135 9 Maintenance and Repair Introduction to Maintenance The module is designed for easy repair The most frequent repairs such as lamp change and flow cell change can be done from the front with the module in place in the system stack 136 Agilent 1260 Infinity Variable Wavelength Detector User Manual Maintenance and Repair 9
122. umps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G4220A B Bin Pump 2 No Yes Yes No Yes G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves G1377A HiP micro ALS G2258A DL ALS G4226A ALS 2 Yes No Yes No Yes Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes Agilent 1260 Infinity Variable Wavelength Detector User Manual 23 1 Introduction to the Variable Wavelength Detector Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED 24 The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter mo
123. ure A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Exchange the leak sensor 104 Agilent 1260 Infinity Variable Wavelength Detector User Manual Error Information 7 Compensation Sensor Open The ambient compensation sensor NTC on the VWM board in the detector has failed open circuit The resistance across the temperature compensation sensor NTC on the VWM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective VWM board Exchange the main board Compensation Sensor Short The ambient compensation sensor NTC on the VWM board in the detector has failed short circuit The resistance across the temperature compensation sensor NTC on the VWM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective VWM board Exchange the main board Agilent 1260 Infinity Variable
124. ver use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 Infinity Variable Wavelength Detector User Manual 39 2 Site Requirements and Specifications Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 13 on page 42 allow to place the instrument on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the bench should carry an Agilent system make sure that the bench is designed to bear the weight of all modules The detector should be operated in a horizontal position Environment 40 Your module will work within specifications at ambient temperatures and relative humidity as described in Table 13 on page 42 ASTM drift tests require a temperature change below 2 C hour 3 6

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