Plant Prep Adem-Kit Protocol
Contents
1. 12 tip comb for 96 Deewell plate Cat 21103 6 Start the Plant Prep protocol with the AutoMag Instrument and load the plate and elution strip Switch ON the AutoMag Instrument and make sure that vou are using the 12 pin magnet head and heating block Start the Plant Prep protocol Insert the plate and elution strip into the instrument as indicated on the AutoMag Instrument displav and press OK Make sure that the elution strip is placed in the correct direction into the elution block Ensure that the perforated end is facing towards the user 7 After the run is completed remove the plate and store the purified DNA When the protocol is completed remove the plate and elution strip according to the instructions on the AutoMag Instrument displav and turn off the instrument Version 1 1 10 Plant Prep Adem Kit NOTE Store or analyze the purified DNA accordingly If DNA is not analyzed immediately store it at 4 C for up to 24 hours For longer period consult laboratory guidelines Freezing samples at 20 C has been shown to preserve DNA for longer periods of time Table 1 Summary of plate and elution strip content Plate name Row Row name Content Reagent Sample and tvpe volume per well Lvsate 400ul LB Buffer 250ul Microtiter Isopropanol 250ul deep well 96 plate Silica MasterBeads 15ul Tip 12 tip com Empt Wash 1 Rene 5OOyI Washin Wash 2 rs hu 500ul Wash 3 70 ethanol 500ul Empty Empty Empty Empty Em2. The products are warranted to the original purchaser only to conform to the quality and contents stated on the vial and outer labels for duration of the stated shelf life Ademtech s obligation and the purchasers exclusive remedy under this warranty is limited either to replacement at Ademtech s expense of any products which shall be defective in manufacture and which shall be returned to Ademtech transportation prepaid or at Ademtech s option refund of the purchase price Claims for merchandise damaged in transit must be submitted to the carrier Ordering Information e Ademtech Kits CAT NO PRODUCT PACKAGE SIZE 06220 Plant Prep Adem kit Plant Prep Adem Kit 6x 96 e Instrument and consumables CAT NO PRODUCT PACKAGE SIZE 21101 AutoMag Instrument Each 21102 Microtiter Deepwell 96 plate 50 pcs 21103 12 tip comb for 96 Deepwell plate 50 pcs 21104 Elution strip 40 pcs 21105 Combi pack for 96 Deepwell plate Each Version 1 1 13
3. Plant Prep Adem Kit NOTE 1 Properly stored Kits are guaranteed until the expiry date Note that shipping is realized at room temperature and will not affect stability All components of the kit have been prepared under nucleases free conditions and have been thoroughly tested to ensure optimal performance NOTE 2 Storage conditions Before using the kit it is recommended to take out the reagents in advance and check if there are any precipitates If the Buffers present precipitates place them at room temperature and eventually put them at 37 C IMPORTANT Do not freeze the magnetic particles 3 Equipment and reagents to be supplied by the user When working with chemicals always wear a suitable lab coat disposable gloves and protective goggles To avoid contamination of your sample wear face mask Reagents e Isopropanol e 96 100 ethanol e 70 ethanol Materials e 56 C heat block thermomixer or water bath e Microtubes e AutoMag Instrument Cat 21101 e Microtiter Deep well 96 plate Cat 21102 e 12 tip comb for 96 Deepwell plate Cat 21103 e Eution strip Cat 21104 Version 1 1 5 Plant Prep Adem Kit Plant Prep Adem Kit Protocol The standard protocol is appropriate for all kind of sample types such as leafs seeds flour Plant Prep Adem Kit is developed for the purification of DNA from ground plant material using paramagnetic particles Recommended quantities Whe
4. Plant Prep Adem Kit AutoMag Solution Cat 06220 06221 Instruction manual for automation protocol ADEMTECH SA Parc scientifique Unitec 1 4 all e du Doyen G Brus 33600 PESSAC France Tel 33557020201 Fax 3355702020 Visit our Web site www ademtech com Plant Prep Adem Kit Version 1 1 Plant Prep Adem Kit Table of contents 3 Introduction 4 1 Description 2 Product component and storage conditions 4 3 Equipment and reagents to be supplied 5 Plant Prep Adem Kit Protocol 6 1 Reagents preparation 2 Perform Lysis 3 Instruction for DNA extraction Troubleshooting 12 Warranty 13 3 Ordering Information 1 Version 1 1 3 Plant Prep Adem Kit Introduction 1 Description Plant Prep Adem Kit is designed for rapid automated purification of DNA from plant materials The DNA purified with the Plant Prep Adem Kit is of high quality and free of proteins nucleases and other contaminants or inhibitors It is therefore suitable for direct use in many different downstream applications such as PCR polymerase chain reaction 2 Product Component and storage conditions Kit Content Each Plant Prep Adem Kit contains sufficient reagents to perform 96 samples using the following standard protocol Item Plant Prep Adem Kit Elution Buffer 10mL RNase 400uL Plant Prep Adem Kit 06220 Silica MasterBeads 2 8C Storage conditions The kits are shipped at room temperature Version 1 1 4
5. a microtube 2 Add 300ul of Plant Lysis Buffer to the microtube containing the ground sample AN MMA CHEMICAL HAZARD Plant Lysis Buffer in contact with acids or bleach liberates toxic gazes Harmful if inhaled absorbed through the skin and swallowed Clause eye skin and respiratory tract irritation DO NOT ADD acids or bleach to any liquid wastes containing this product Avoid breathing vapour Do not taste or swallow Use with adequate ventilation Avoid contact with eyes and skin Read the MSDS and follow and handling instructions Wear appropriate protecte eyewear clothing and gloves Version 1 1 7 Plant Prep Adem Kit 3 Add 4uL of RNase 4 Close the tube mix well with a vortex and place it in a thermomixer then incubate at 56 C and 1000rpm for 60minutes IMPORTANT You can use a heat block or a water bath instead of a thermomixer 5 Centrifuge the Ivsat 20 minutes at 5600g 6 Perform DNA extraction with 150ul of supernatant 3 Instruction for AutoMag Instrument with 12 pin magnet head and 96 deep well plates for DNA extraction from forensic samples These instructions are for the DNA extraction from 150ul of lysate using Plant Prep Adem Kit Cat 06220 and the AutoMag Instrument Cat 21101 with a 12 pin magnet head and 96 deep well plates AutoMag Instrument is a low to medium throughput purification system with a magnetic particle processor for DNA purification kits With the Au
6. at 30mg Sunflower 1 2 punches 1 Reagents preparation AN warnine CHEMICAL HAZARD Plant Lysis Buffer in contact with acids or bleach liberates toxic gazes Harmful if inhaled absorbed through the skin and swallowed Clause eye skin and respiratory tract irritation DO NOT ADD acids or bleach to any liquid wastes containing this product Avoid breathing vapour Do not taste or swallow Use with adequate ventilation Avoid contact with eyes and skin Read the MSDS and follow and handling instructions Wear appropriate protecte eyewear clothing and gloves Version 1 1 6 Plant Prep Adem Kit EX wannie CHEMICAL HAZARD Washing Buffer 1 Concentrate causes eye skin and respiratory tract irritation Avoid brething vapour Use with adequate ventilation Avoid contact with eyes and skin Read the MSDS and follow the handling instructions Wear appropriate protective eyewear clothing and gloves e Prepare a 70 ethanol solution e Prepare the Washing Buffers amp Il before first use 1 Add 32mL of 96 100 ethanol to the bottle containing 24mL of Washing Buffer Homogenize the solution 2 Add 39mL of 96 100 ethanol to the bottle containing 17mL of Washing Buffer Il Homogenize the solution NOTE Washing Buffer amp Il are delivered concentrated Before the first use you have to add ethanol in the bottle in the indicated proportion 2 Perform lysis 1 Place the ground sample at the bottom of
7. pty Empty Empty Empty Empty Elution strip Elution Elution Buffer 60uL Version 1 1 11 Plant Prep Adem Kit Troubleshooting Observations Comments and suggestions Error message in Refer to the user manual supplied with your AutoMag instrument display Instrument The tip comb holder Switch the instrument OFF and ON and try again If lifting mechanism is the error appears during initialization or is otherwise out of position repeated contact service The turntable Switch the instrument OFF and ON and try again If rotating mechanism the error appears during initialization or is otherwise is out of position repeated contact service The magnet head Switch the instrument OFF and ON and try again If ER sitet the error appears during initialization or is otherwise Le repeated contact service position The plastic tip comb Check if the tips are presents If it looks all right turn is not attached to the holder ON and OFF and run the check protocol Review protocol steps and Nor or low yield of Biological sample reagents additions DNA contains no or low amount of DNA Extract DNA from a different cutting from the sample Version 1 1 12 Plant Prep Adem Kit This product is only for use in research The purchaser is responsible to validate the performance of this product for any particular use and to use the product in compliance with any applicable regulations
8. toMag Instrument customers can process up to 12 samples per run with a working volume up to 1ml In addition it is possible to run two purification methods sequentially without interruption raising the throughput up to 24 samples AutoMag Instrument Cat 21101 Version 1 1 8 Plant Prep Adem Kit Instructions 1 Prepare the lysate according to the instructions on pages 7 and 8 2 Take one empty Microtiter Deep well 96 plate and one Elution strip Microtiter Deep well 96 plate Elution strip Cat 21102 Cat 21104 3 Prepare the plate Add the following reagents to the rows Note that row B is reserved for the tip comb and should be left empty Note that rows F G and H are left empty Plate name Row Row name Content Reagent Sample and tvpe volume per well Lvsate 150uL Isopropanol 150uL Silica Microtiter MasterBeads 15uL Deep well 96 i 12 tip comb Empty plate Washing Buffer l Washin Buffer Il 70 ethanol 500ul Empty Empty Empt Empt Empt Empt 500uL Version 1 1 9 Plant Prep Adem Kit 4 Fill the Elution strip as follows Make sure that the elution strip is placed in the correct direction into the elution block Ensure that the perforated end is facing towards the user and the Elution Buffer is pipetted into the correct wells Elution strip Content Reagent volume per well Elution strip Elution Buffer 60 100uL 5 Placea 12 tip comb for 96 Deepwell plate into row B of the plate
Download Pdf Manuals
Related Search