User Manual 420A
Contents
1. e 7 Chapter 2 Setting orar 8 2 1 Setting Up The Wire Myograph Sy Ste Mirra AAA Ru E GERBER IUE 8 22 INE TOROS SC Gil UO et ar e AAA a 9 Chapter The Wire Interface 10 3 1 General description on how to Navigate the touch screen doncc A n 10 3 2 POWEr ISA Set eutectic a DOCE tak nebo ek DUI I UK PO EY VR RU ENIM GUN EFI EL Ra NEMA UR KIA RI DEREN N E 10 A A A e O RU O O AE A E E E EE 11 A E A A AT TU O Pen RE A AA 10 Heat MiS ETT Um me M 12 20 nimer and Buzzer IE Tm AAN ADN Rc 12 SMS gt PP Tcr 13 SPORE Calibration MENU aaa A ON Satine 13 POL cogendi Im dM 16 So Select Analog Output Optional rara DANA ARS AAA ANA 17 A PP e E E E E 19 Appendix 1 System specifications acia e 20 NOTO Suicide iii 21 CONTENTS 3 SAFETY The Wire Myograph System has been designed for use only in teaching and research applications It is not intended for clinical or critical life care use and should never be used for t2. TO RECORD A NOISE FREE SIGNAL IT IS IMPORTANT TO CONNECT THE WIRE INTERFACE GROUND CONNECTION TO THE DATA ACQUISITION SYSTEM S GROUND CONNECTION 1 Wire Myograph to Wire Interface connection Connect the Wire Myograph to the Wire Interface using the grey 44 25 pin connector cable The end of the cable with the temperature probe is attached to the Wire Myograph 2 Wire Interface to computer connection Data acquisition is possible either by connecting the Wire Interface directly to a computer or through a data acquisition system able to collect voltage output O 2 5volts such as PowerLab data acquisition system or similar system Direct computer USB connection for Labchart AD Instruments users latest version of Labchart Connect the Wire Interface to the computer with the USB cable from the back panel to the USB port Analog Data Output through a PowerLab or similar data acquistion system optional Connect the Wire Interface to the analog data acquisition system using BNC cables Connect Rec 1 Chamber 1 on the Wire Interface to Input 1 on the analog data acquisition system Rec 2 Chamber 2 to Input 2 on the analog data acqui sition system 2 and so forth Connect the analog data acquisition system to one of the USB ports on the computer 8 WIRE MYOGRAPH SYSTEM 420A USER MANUAL 3 Turn on the Power Turn on the power to the Wire Interface at the power switch and then turn on the computer Start the data acquis
3. repeat the calibration value 4 00 Probe Temp 25 0 NEXT STEP 3 7 3 Select Analog Output optional SELECT ANALOG OUTPUT The Select Analog Output Menu determines what will be sent to the BNC Select output 1 analogue output 1 REC 2 REC 3 REC 4 at the back of the Wire face There are 4 analog outputs and each output is individually program Select output 2 SELECT mable Any change made to the analog output will affect the data sent from the Wire Interface to a data acquisition system such as AD Instruments Select output 3 SELECT PowerLab Therefore remember to check the data acquisition system s set up when a change is made The analog output is working in range of 2 5V Select output 4 SELECT to 2 5V Use SELECT to select the out channel number to be changed Press ENTER to go to the output set up 17 Use SELECT to select the out channel number to be changed Press ENTER to go to the output set up Use the UP and DOWN arrow or DEFAULT to change select a new set up The following parameters in the Wire Interface can be selected as output to the analog channels Force Near Force Far Temperature Probe Chamber Temperature pH1 Optional Digital Output 1 Digital Output 2 Digital Input 1 Digital Input 2 When the chosen parameter is selected press ENTER to save the value in the memory The output range is 2 5V to 2 5V The user can select the parameter v
4. MESSAGE OFF IS DISPLAYED ON THE MAIN PAGE OF THE WIRE INTERFACE EVEN THOUGH THE CHAMBER IS PLUGGED IN AT THE REAR OF THE WIRE INTERFACE THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED IN ADDITION IF THE FORCE READING S APPEAR YELLOW CANNOT BE RESET TO ZERO AND THE TRANSDUCER CANNOT BE RECALIBRATED THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED Step no 5 Turn the heat on Wait until the temperature is stable Temperature setpoint 37 0 C Probe temperature 36 8 HEAT OFF NEXT STEP otep no 1 5 Make sure that the transducer is not subjected to any force When the relative force reading is stable go to next step Force Chamber 3261 NEXT STEP CHAPTER3 15 Step 5 At this step place the 2 gram weight at the pan on the calibration T balance closest to the transducer over the transducer see figure 3 1 to NEAR CALIBRATION simulate the stretch created by the contraction of a mounted ring prepara tion Step no ge 5 Carefully place the 2 g weight on the pan When the relative force reading is stable go to next step Remember a 2 gram weight in a 90 vector is divided and the transducer will only detect 1 gram or 9 81 mN of force The weight placement should cause a positive increase in the four digit number Wait at least 30 to 45 seconds for the applied force to stabilize before pressing NEXT STEP Force Chamber 3346 Step 6 is
5. A Step 1 Involves setting up the chamber for calibration Fill the chamber to a normal level with double distilled water Move the jaws apart Press NEXT STEP IMPORTANT TURN ON THE HEAT AND SET THE TEMPERATURE AT THE LEVEL AT WHICH THE EXPERIMENTS WILL BE PERFORMED Step 2 Involves setting up the Calibration Kit for the actual force calibra tion Make sure that the T balance pin is placed between the wire and the jaw as illustrated in figure 3 2 Carefully move the calibration bridge until the T balance pin is placed freely between the wire and the jaw which means it does not touch the wire or the jaw see figure 3 2 NOTE THE WEIGHT SHOULD NOT BE PLACED ON THE T BALANCE YET Press NEXT STEP when the calibration kit has been properly placed 14 WIRE MYOGRAPH SYSTEM 420A USER MANUAL NEAR CALIBRATION Step 5 C5 Follow the weight calibration procedure in the User Manual Fill the chamber with double distilled water When ready go to next step NEXT STEP otep no DOM 5 5 Place the calibration bridge on the myograph Place the bridge so the pin does not touch the wire Jaw When ready go to next step NEXT STEP Figure 3 2 Illustration of how to fit the T balance between the wire and the gap in the jaw support Step 3 Initiates the heating process for the chamber In order for the cali bration to be accurate the transducers must be heated to the experimental temperature to be us
6. DANISH MYO TECHNOL Ost WIRE MYOGRAPH SYSTEM 420A USER MANUAL User Manual Version 1 0 TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 35 are trademarks of ADInstruments Pty Ltd DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with Wire Myograph system 420A No part of this document may be reproduced by any means without the prior written permission of Danish Myo Technology A S Copyright Danish Myo Technology A S DANISA MeO TECHNOL OGS 2 WIRE MYOGRAPH SYSTEM 420A USER MANUAL CONTENTS e 2 AA nn A EE o E no 4 Declaration of COD FOE MIN srta oi A in 4 Unpacking the Wire Myograph 5 Chapter 1 Wire Myograph System OVO Vi Word DUM RU ON GR RUN ODER M ERN N A 6 Li Wire Ale roD a DEL Cal MIU 6 1 2 Dual Wire Myograph Ona O AN
7. Type login code to get Access o 2 E HY Code init value 51761 OLLU CHAPTER 3 19 APPENDIX 1 SYSTEM SPECIFICATIONS Technical specifications Wire Interface Voltage Current max Dimensions Net weight Environmental humidity Environmental operating temperature Environmental storage temperature USB connector 4 Analog outputs External 100 240VAC to 24VDC ADAPTER 10 3 3Amps at 24VDC 34 x 25 x 15 cm LxDxH 5kg 20 to 80 RH none condensing 15 to 40 C 4 to 70 C For Download of Firmware and connection to PC Software BNC connector at the back used to Connect to ext acquisition system Analog output range Range is 2 5Volt Technical specifications Dual Wire Myograph 420A Vessel size Chamber Chamber material Chamber volume Chamber suction Chamber cover Chamber gassing Force range Force resolution Micrometers Force calibration Heating Temp range Temp resolution Temp probe Output reading Analogue output Serial output Voltage Ambient temp Data on the transducer Max Range Operating temperature Frequency range Optional accessories Enable pH electrode on the Wire Interface range temp correction Electronic valve gt 60 um Dual independent or dependent Acid resistant stainless steel Max 10 ml No With connection for suction and gassing Yes 100 to 200 mN 0 1 mN Manually operated precision micromete
8. Wire Interface e Power supply the shape of the AC plug varies by country be sure that the plug has the right shape for your location Wire Myograph 420A e Wire Myograph connection cable with a temperature probe e Two sets of stainless steel mounting jaws with supports e Chamber cover Accessories e 1x calibration kit including bridge T balance and 2 gram weight e 1x spool of 40 um stainless steel wire e 1xtube of high vacuum grease e 1x tube of grease for linear slides e 5xspare screws for mounting of jaws e 1x bath divider e 3 x Allen keys e 1x small screwdriver e 2x40 mm funnel Optional Accessories e PowerLab e LabChart e DMT Device Enabler e Stimulator pH meter e Vacuum Pump e Electronic vacuum valve e Waste bottle e Gas supply manifold UNPACKING THE WIRE MYOGRAPH SYSTEM 5 CHAPTER 1 WIRE MYOGRAPH SYSTEM OVERVIEW 1 1 Wire Interface front and rear 88 22 mN 46 1 MN n Near chamber force Far chamber force Probe temperature OFF pH1 is not mounted Touch display Figure 1 1 Wire Interface front Analogue recorder output connection to Grounding connect to external data acquisition software optional Data Acquisition e POWER 1 REC 2 ON OFF switch e RECA REC 3 JAI p a pa M y v We Connection port for pH electrode optional 24V DC port e 4 for p
9. alue that gives 2 5V and the value that gives 2 5V In this example 100mN is 2 5V and 100mN is 2 5V Selecting asymmetrical values is also valid They can be 2 5V OMN and 2 5V 100mN NOTE REMEMBER TO PRESS ENTER TO SAVE THE LINE CHANGES IN MEMORY 18 WIRE MYOGRAPH SYSTEM 420A USER MANUAL Output 1 Force SELECT Setting 1 2 5V 100mN SELECT Setting 1 2 5V 100mN SELECT o UIS ATTE ES Output 1 Force SELECT Setting 1 2 5V 100mN SEEGI Setting 1 2 5V 100mN SELECT DEFAULT 3 7 4 Interface Settings The Interface Settings sub menu has an additional three sub menus Temperature Difference pH Set up Factory Diagnostics 3 7 4 1 Temperature Difference offset The temperature difference function allows the user to fine tune the tem perature set point of the system Although the temperature set point for the system can be set in the Heat Menu there may be a small discrepancy between the actual temperature of the system and the defined set point The user can adjust the temperature of each chamber individually to fine tune the temperature setting so the exact temperatures are achieved in each chamber This is referred to as a temperature offset TEMP OFFSET ON CHAMBER The SELECT and ALL functions are the same in this menu as for previously described menus Pressing ENTER will store the numbers in memory for future experiments 3 7 4 2 pH Set up Menu The pH calibration is a 2 poi
10. ed This accommodates heat induced expansion of the electronic parts in the transducer Otherwise inaccurate readings and transducer drift may occur producing errors into the experiment Therefore now turn the heat on by pressing HEAT ON Covering the chamber with the chamber cover will expedite the heating Place the temperature probe into the chamber for the first calibration to determine when the chamber has reached the target temperature Heating will take about 20 to 30 minutes for the chamber and transducers to reach 37 C with the chamber covers in place Once the chamber are heated and have reached the target temperature press NEXT STEP Step 4 is the first step in the actual force calibration process A four digit number will be displayed in blue at the bottom of the screen If nothing has been disturbed during the heating process the zero O gram or 0 0 mN calibration should be stable as indicated by the four digit number Wait at least 30 to 45 seconds until the four digit number is stable before pressing NEXT STEP NOTE NORMAL OPERATING VALUES FOR THE FORCE TRANSDUCER DURING CALIBRATION SHOULD BE BETWEEN 3000 AND 3500 IF THE VALUE IS 0 A SINGLE DIGIT OR A THREE DIGIT NUMBER THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED IF THE VALUE IS LESS THAN 2000 OR GREATER THAN 4500 BUT STILL A FOUR DIGIT NUMBER THE FORCE TRANSDUCER IS BROKEN BUT SHOULD BE ABLE TO BE REPAIRED BY A TEMPERATURE COMPENSATION DONE BY DMT IF THE
11. er and entered manually in the Manual temperature line The calibration procedure is listed in 4 individual steps and needs to be performed one by one to calibrate the pH Step 1 Involves cleaning the pH electrode and the temperature probe with double distilled water When ready press NEXT STEP pH CALIBRATION sen BO OG Follow the pH calibration procedure in the User Manual Clean the pH electrode and the temperature probe When ready go to next step NEXT STEP 16 WIRE MYOGRAPH SYSTEM 420A USER MANUAL Step 2 Place the pH electrode and temperature probe in the high buffer solution here pH 7 and turn on stirring When the relative pH output inthe pH CALIBRATION blue line is stable press NEXT STEP sen ABONO Apply buffer with the High pH value to the pH and temp probe When the pH reading is stable go to next step pH readings 1863 Step 3 Place the pH electrode and temperature probe in the low buffer solution here pH 4 and turn on stirring When the relative pH output in the blue line is stable press NEXT STEP pH CALIBRATION Step no 5 Apply buffer with the Low pH value to the pH and temp probe When the pH reading is stable go to next step pH readings 883 Step 4 The calibration is now finished The readings in the two bottom lines are the actual pH and temperature readings pH CALIBRATION Step no The pH electrode is now calibrated if OK go to next step else
12. hese purposes Nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the apparatus the internal electronics pose a risk of electric shock e Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus e Do not block any ventilation openings Install in accordance with the manufacturer s instructions e Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time The Wire Interface is delivered with an external 100 240VAC to 24VDC adapter Protect the power adapter and cord from being walked on or pinched Particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped EC DECLARATION OF CONFORMITY Danish Myo Technology A S Certify and declare that the following apparatus Wire Myog
13. ition pro gram on the computer and the Wire Myograph System is now ready for use 4 Gas supply Connect the pipes for gas supply on the Wire Myograph chamber cover see figure 2 2 to an adjustable gas supply using thin silicone tubing 5 Suction connection Connect the suction pipes on the Wire Myograph chamber cover see figure 2 2 to a vacuum pump via a Suction bottle and the electronic vacuum valve as illustrated in figure 2 1 6 pH electrode optional Connect the pH meter to the pH port on the rear of the Wire Interface Suction pipes for connection to vacuum pump Access for temperature probe A D Access for reagents and buffer Figure 2 2 Chamber cover for Wire Myograph 420A 2 2 The first force calibration Prior to shipping the Wire Myograph System has gone through two days of continuous testing including final force calibra tions However DMT recommends that a new force calibration is performed before using the Wire Myograph System The force calibration procedure is described in detail in chapter 3 7 1 CHAPTER2 9 CHAPTER 3 THE WIRE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Wire Interface 3 1 General description on how to navigate the touch screen Menus on the Wire Interface are all accessible by a touch screen To access a menu simply touch the
14. n Main Menu the bell icon also turns green SET TIMER Set Timer 00 00 00 3 7 Settings Menu The Setting Menu contains several submenus These submenus include Force calibration pH calibration Record out select Interface settings 3 1 Force Calibration Menu Prior to shipping the Wire Myograph System has gone through two days of continuous testing including final force calibrations However DMT FORCE CALIBRATION recommends that new force calibrations are performed before starting to use the Wire Myograph System for the first time Force calibration near SELECT Begin the calibration procedure by pressing Force Calibration in the Set Force calibration far ET tings Menu The sub menu will list 2 chambers for calibration The calibra tion procedure is listed in 6 individual steps and needs to be performed to calibrate the system Before starting the force calibration the following is performed Place the calibration bridge and T balance on the Wire Myograph see figure 3 1 allowing it to be warmed up together with the Wire Myo graph Turn on the heating in the Heat sub menu to the appropriate temperature used during your experiments e Mount a 40 um stainless steel wire on the jaws connected to the force transducers CHAPTER 3 13 The 2 g weight is placed on this pan Figure 3 1 Force calibration set up showing placement of the calibration bridge and T balance for Dual Wire Myograph 420
15. nt calibration In the pH set up menu the two pH values used for the 2 point pH calibration should be selected The default in the pH Calibration setup is the pH values 4 and 7 If using pH buffer with different pH values than 4 and 7 for the pH calibration enter the appropri ate pH The temperature is an important parameter in the calibration formula and is obtained automatically if the temperature compensation it is set to AUTO as shown If MANUAL is chosen a manually set temperature is used in the pH calibra tion formula and the temperature probe is not used In the MANUAL mode the temperature of the calibration buffer is measured with a thermometer and entered manually in the Manual temp Value line 3 7 4 3 Factory Diagnostics Entering Factory Diagnostics will display the Login code to diagnostics window This window is only for trained technicians and used for diagnos tics and troubleshooting purposes The general user will not have access to this window Entering the proper five digit login code however will allow the trained technician access to diagnostics panels that will provide information during a malfunction or mechanisms to change other settings controlled by the onboard computer INTERFACE SETTINGS TEMPERATURE DIFFERENCE SET UP FACTORY DIAGNOSTICS TEMP OFFSET Chamber 1 2 C Bottle 1 1 C pH SET UP MENU Low buffer pH 4 0 High buffer pH 7 0 Temperature comp AUTO Manual temp Value 20 C
16. on or to change the temperature for the system press HEAT in the Main Temperature setpoint 37 0 C Menu The Heat Menu will be displayed allowing the user to change the system temperature as well as turn the heat on or off Pressing DEFAULT will automatically reset the temperature setpoint to 37 C Manually change the temperature by pressing UP or DOWN arrows Pressing ENTER will save the new temperature setpoint Probe temperature 36 6 C To turn the heat on press ON and it will turn green The system will heat to the desired temperature setpoint In the Main Menu the heat icon turns green when the heat is on Temperature setpoint 37 0 pl Probe temperature 36 6 C ON SET TIMER 3 6 Timer and Buzzer Menu The timer and buzzer are controlled from this menu Use SELECT to program the timer The timer can be programmed in the interval from O to 23 59 59 Set Timer 00 00 00 Hours minutes seconds When Set Timer is selected use the UP and DOWN arrows to program the timer Use the RIGHT arrow to move from hours minutes and seconds Press ENTER to save the programmed time CLR TIMER ENTER 12 WIRE MYOGRAPH SYSTEM 420A USER MANUAL To start the timer press ON and it will turn green SET TIMER When the timer reaches zero it automatically switches to OFF Set Timer 00 00 00 If the buzzer is turned on it will make a tone when the timer reaches zero To activate the buzzer press ON and it will turn green I
17. ower e USB connection port e for computer Connection port for Wire Myograph Figure 1 2 Wire Interface rear WIRE MYOGRAPH SYSTEM 420A USER MANUAL 6 1 2 Dual Wire Myograph 420A Port for connection to Wire Interface Micrometer Chamber separator e Wire jaw connected to micrometer e 1 Allen screws for alignment of the jaws 4 on each side 0 O 53 BANW NM eR OS ee e Window the bottom of the chamber E under the wire jaws MU DET e Wire jaw connected to force transducer SAA Figure 1 3 Dual Wire Myograph 420A with close up of the chamber CHAPTER 1 7 CHAPTER 2 SETTING UP 2 1 Setting up the Wire Myograph System Wire Interface rear panel m E m Power supply Data Acquisition System via BNC cables or USB X Oo a amm JL Electronic vacuum valve e Y Vacuum Pump pH Meter Gas supply manifold Suction Bottle Dual Wire Myograph Figure 2 1 Example of a complete Wire Myograph System 420A Oxygen Carbogen Compressed air Figure 2 1 is a complete set up for the Wire Myograph System The set up includes optional equipment such as pH electrode for measuring pH in the buffer an electronic vacuum valve suction bottle vacuum pump and a computer and Data Acquisition System IMPORTANT
18. raph System DMT420A Restrictive use Only for laboratory use Manufactured by Danish Myo Technology A S Skejbyparken 152 8200 Aarhus N Denmark Conforms with the essential requirements of the EMC Directive 2004 108 EC Based on the following specifications applied by EN 61326 1 2006 EN 61326 2 6 2006 EN 61326 2 6 Corr 2007 And with the LVD Directive 2006 95 EC Based on the following specifications applied by EN 61010 1 2010 EN 61010 2 030 2010 General warnings regarding EMC Do not use this device in close proximity to sources of strong electromagnetic radiation e g unshielded intentional RF sources as these may interfere with the proper operation 4 WIRE MYOGRAPH SYSTEM 420A USER MANUAL UNPACKING THE WIRE MYOGRAPH SYSTEM Please take a few minutes to carefully inspect your new Wire Myograph System for damage which may have occurred during handling and shipping If you Suspect any kind of damage please contact DMT immediately and the matter will be pursued as quickly as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Wire Myograph System Please contact DMT Sales Department for packing instructions if the original packing material is unavailable After unpacking your new Wire Myograph System please use the following list to check that the system is complete
19. rce 20 2 mN Far chamber force 10 2 mN Probe temperature 36 8 C value 6 81 TIMER ZERO HEAT BUZZER SETTING Buzzer icon This icon indicates the status on the Buzzer If the icon is grey the Buzzer is OFF If the icon is green the Buzzer is ON active and will make a sound when the timer reaches zero OFF ON Timer The Timer is a countdown timer that can be set to maximum 24 hours If the 00 00 00 Buzzer is activated it will buzz when the timer reach zero Heat icon This icon indicates the status of the chamber heat 3 If the icon is grey the Heat is OFF If the icon is green the Heat is ON active OFF ON Four sub menus are accessible from the Main Menu screen Zero Menu Heat Menu Timer Alarm Menu Setting Menu CHAPTER 3 11 3 4 Zero Menu SET FORCE TO ZERO This menu is used to zero the output of the transducers When using a data acquisition program like LabChart by AD Instruments this feature will Near chamber force 2 36 mN reset the baseline of the chart traces without affecting the calibrations or physically changing any pre load tensions placed on the mounted vessels The channels can be changed individually by pressing SELECT or all at once by pressing ALL Pressing ENTER will execute the zero function and return the user to the Actual Force display SELECT Far chamber force 3 38 mN SELECT 3 5 Heat Menu The heating temperatures are controlled from this menu To turn the heat
20. rs Manual Built into chamber independent of superfusion Ambient temp 50 C 0 1 C External Force mN Up to four outputs 1 0 V full scale for all acquired signals user defined Serial interface RS232 RS485 100 to 240 VAC auto 50 60 Hz via external power supply 15 30 C 200mN AS OOOO O 20Hz pH O 14 50 100 to 240 VAC auto 50 60 Hz via external power supply 20 WIRE MYOGRAPH SYSTEM 420A USER MANUAL NOTES NOTES 21 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com salesQdmt asia com supportedmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China 8510 215425 1330 Fax 86 21 5877 0063 www dmt asia com sales dmt asia com Support dmt asia com DMT USA Inc 525 Avis Drive Suite 10 Ann Arbor MI 48108 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com supportodmt usa com
21. screen When a setting needs to be changed TEMP OFFSET press SELECT beside the line to be changed Chamber 1 2 C SELECT SELEC Bottle LTG SELECT The line to be modified will turn blue indicating that the Wire Interface is waiting for input When ALL is chosen all lines corresponding to all four channels will turn blue ALL Changing the numeric value for the chosen parameter can be done by touching UP or DOWN arrows LA J LY Once the desired setting has been chosen pressing ENTER will lock the selection and be stored in memory ENTER Pressing the white X in the red box will exit the menu and take you automati cally to the Main Menu MULTI INTERFACE 3 2 Power up screen After turning on the Wire Interface an introduction screen appears It dis Multi Myograph System plays the product system model no software version number date and the Model 420A system ID number Software Revision 02 04 The system is auto initializing while this screen is displayed 10 WIRE MYOGRAPH SYSTEM 420A USER MANUAL 3 3 Main Menu The Main Menu gives a good overall status on how the Wire Interface is working It displays the force pH probe temperature timer and the active status buzzer timer heat Icon for Heater ON OFF K Timer M Icon for Buzzer ON OFF le MAIN MENU A 00 00 00 A If ON the icon is green Near chamber fo
22. to verify that the calibration was performed correctly The Force Chamber reading should be 9 81 0 1 mN If the Force Chamber reading NEAR CALIBRATION is off by more than 0 1 mN then remove the weight press BACK to return to Step 4 and repeat the calibration process If the Force Chamber reading is Step no 5 satisfactory then press NEXT STEP to end the calibration The transducer is now calibrated Force read out should be 9 81 mN After calibrating the force transducer carefully remove weight T balance 0 1 mN If OK go to next step and calibration bridge The Wire Myograph System is now ready for longitu Otherwise repeat the calibration dinal force measurements Force Chamber 9 81 mN BACK NEXT STEP Before the pH calibration is performed be sure to select the way the pH pH SETUP MENU electrode is intended to be used See sub menu pH SETUP under Wire Inter 3 7 2 pH Calibration Procedure face Settings chapter 3 6 5 2 Low bufferpH 4 0 SELECT The temperature is an important parameter in the calibration formula High buffer pH 7 0 SELECT is obtained automatically if the Temperature compensation has AUTO se lected as shown Temperature comp AUTO SELECT If MANUAL is chosen the Manual temperature is used in the pH calibration Manual temp Value 20 C SERE formula and the temperature probe is not used In the MANUAL mode the temperature of the calibration buffers is meas ured with a thermomet
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