Home

CryoMed Controlled-Rate Freezers - User Manual

1. BILL DF MATERIALS ITEM PART ND PART DESCRIPTIDN QTY l 20017 1 4 20 X 3 4 ZP HH CAP SCREW 2 2 22052 8 32 X 3 8 SS PHP SCREW 8 3 23001 46 32 ZP LKWASH HEX NUT 4 4 111035 FLAT SPRING CLAMP 4 5 190611 STAINLESS STEEL HINGE PIN 2 6 6850012 250 DIA EXTERNAL RETAINING RING 2 7 730038 247 ID TEFLON FLAT WASHER 4 8 730063 FLAT WASHER SHIM 2 g 890005 INTERIOR DUCT WRAP O 1890005 INTERIOR LEFT SIDE GRILLE I 890007 INTERIOR RIGHT SIDE GRILLE 2 1890016 SIDE ACCESS PANEL SUB ASSEMBLY 3 1890053 SHIPPING BRACKET 4 DOOR ASSEMBLY 5 1890035 0 6CF INTERIOR DUCT WRAP 6 1890044 1 7CF INTERIOR DUCT WRAP NOT SHOWN 130059 STANDARD AMPULE STOPPER 2 130060 STRAW STOPPER 2 430326 CUROSET 13A 125V 16 3 USA DOMESTIC MODELS 430108 CURUSET 10A 250V EUROPEAN EXPORT MODELS 4000385 2ML SAMPLE THERMOCOUPLE SENSOR 4000401 B FT TRANSFER HOSE 4000566 THERMAL PRINTER PAPER 5 ROLLS 7007452 CONTROLLED RATE FREEZER MANUAL THERMAL PRINTER MANUAL CABINET ASSEMBLY 7452 201 4 REV PAGE 2 OF 2 7 12 7007452 CryoMed Freezer Thermo Scientific Section 7 Parts List MOUNTS TD UPPER LEFT CORNER DN CEILING OF INTERIUR CHAMBER
2. 43 I D IA a El NCE CHARI 45 45 WIRE GAUGE COLOR 47 I 16 BROW 2 16 BLUE 48 j 16 GRN YEL AL 4 16 BROW 5 6 BLUE 50 D 24 WHITE 7 24 WHITE 7 B 22 RED 52 7 22 BLAC ll 16 BLAL E IX o n 23 12 16 BLA 54 13 16 W IT 14 16 BLAC 55 15 16 BLUE 16 16 GRN YEL 26 17 18 RED 57 18 18 ED i 19 18 GRN YE 98 20 18 RED 59 2 i E e 22 18 GRN YEL 50 61 62 63 64 65 66 EXTERNAL CONNECTIONS eh D 68 EXTERNAL P C CONNECTION co FEMALE SUBMINTATURE D CONNECTOR o PIN 2 TRANSMIT DATA 10 PIN 3 RECEIVE DATA PIN 5 SIGNAL GROUND 5i t 72 ANALOG RECORDER OUPUT O0 1OVDC NONLINEAR 74 FEMALE SUBMINIATURE D CONNECTOR E PIN 2 PLUS 74 PIN 4 MINUS 75 76 5 RTT POK AKS ADD TVE PROBE CONNECTIONS f I 4 RIT POK LON ADDED MOTOR FUSE TU DRAWING Electrical Schematic 3 s 18 01 RIT RTT Be CHANGED WATTAGE FROM 1000 2 Fr um 5 9 01 RIT RT BH ADO MOTOR 156123 REV 5 amp J10 PLACE Yodel 1 FS 14913 1970 RIT RTT RU REV PUER CONECTION P S SIK 7450 7452 7454 REV JECN Nd ATE BY CAD APPD DESCRIPTION OF REVISION V m dE vt numm R R qug mener weew we sez TE 7456 and 7458 MA x aqu SO EE GE WTI Ge mE E ELECTRICAL SELLE Con trol Ya le Freezer LEN OBSERVE PRECALITIONS WRITTEN PERMISSION FROM THERMO FORMA DWN RIT CAD R APPD BH UATE 10 6 00 state NONE SE Pir ELECTROSTATIC MATERIAL LIE SENSITIVE DEVICES PAINT COLOR 7450 70 1 D REV 5 DRAWING NLIMBER SIZE E r ThermoForma 7450 70
3. Thermo Scientific 7007452 CryoMed Freezer 7 13 Section 7 Parts List BILL DE MATERIALS ITEM PART ND PART DESCRIPTIUN HTY l 22052 8 32 X 3 8 SS PHP SCREW 4 2 23010 48 32 SS HEX NUT 2 3 23021 8 SS FLAT WASHER 2 4 23080 8 SS SPRING LOCKWASHER 2 5 180250 HOLDER SLEEVE 18 6 180251 SLEEVE RETAINING NUT 18 NOTE QUANTITY IS 180255 sLEEVE PLUG 18 30 FOR 1 2CF UNIT 8 890006 TERIUR LEFT SIDE GRILLE g 890007 TERIOR RIGHT SIDE GRILLE 0 890055 TERIOR CHAMBER SUB ASSEMBLY 890056 0 8CF IVF EXTERIOR CABINET 2 890057 TERIUR DUCT WRAP 3 890058 INTERIOR CHAMBER SUPPORT 4 900179 ALUMINUM HINGE BLOCK 5 DOOR ASSEMBLY 6 1880004 BENT LIPPER RIGHT CHAMBER SUPPORT 5 890059 1 2CF INTERIOR CHAMBER SUB ASSEMBLY 6 890060 1 2CF IVF EXTERIOR CABINET 7 890061 1 2CF INTERIOR DUCT WRAP IVF CABINET ASSEMBLY 7 456 200 1 6 REV 1 AQ 2 F 2 7 14 7007452 CryoMed Freezer Thermo Scientific c 22049 6 32 X3 0 SCREW 4 REN Section 7 Parts List 40205 CONTROL PANEL 101648 LE DISPLAY EDO 0219 I 0 POS FLAT CABLE ASSY l
4. If exposed to liquid or cold gas restore tissue to normal body temperature 98 6 F as rapidly as possible followed by protection of the injured tissue from further damage and infection Remove or loosen clothing that may constrict blood circulation to the frozen area Call a physician Rapid warming of the affected part is best achieved by using water at 108 E Under no circumstance should the water be over 112 F nor should the frozen part be rubbed either before or after rewarming The patient should neither smoke nor drink alcohol Thermo Scientific Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Marietta Ohio 45750 United States www thermo com
5. 12300 FUGE PROTECTOR 6 LING lt ll 9 MAIN BR PRIMARY CONNECTION N w2 CS li A AER SUPPLY SWITCHER W Ba l zu L n f LZ oW 370524 8 PIN PLUGGAR AA REFER TO DETAIL 3 Le lt s YSN Wie A EA EM W 1890017 CONIRUL BURD W lt KW A t cm W 20052 8 32 X VB MAL gt lt o 23059 4 8 LOCKWASHER e uiui 195718 HAIN HARNESS nep SUPPLY 240002 PUSHIN TIE o E SONDA CIMECTIIN 730063 NASHE 2 RED DETAIL 3 TERMINAL BLOCK WIRING DETAIL 1890053 SHIPPING BRACKET 7 Ba SES aac PET E noeooooo92o 20017 1 4 20 Y 3 4 HEX BLT 1 I WI um e a d Te T Ee SC RE WITH IN 1 2 OF TERMINAL BLOCK ed EN WSC Wiring Diagran lt NS NA Em e n I SS TE WIRE FROM THERMOSTAT Mode We INS LA MIRE FRIM MOTOR 71450 100 120V I N El rc B uem EE Controlled Rote S SSS SSD WIES FROM CL SENCO I gt S RED WIRES FROM COOL PLUS SOLENOID Freezer GREEN WIRES FROM SILENDID VALVES 7450 71 1 D REV 2 Page 1 of 2 Thermo Scientific 7007452 CryoMed Freezer 7 15 Section 7 Parts List DETAIL 4 NERTEMP amp P S OUTLET WIRING DETAIL a BLACK ENT Es LIRE REAR AND TOP VIEW RIMP FAN WIRES TO RED AND BLACK GA WIRES u 2001 6 KEPS NUT Eu 200134 FAN 4 492011 Y ES OS SINGLE RNY St d STE L ENTRY WE L m k in ay Sa 32 X 1 1 2 SCREW u x 77 KE d 2303
6. aqoid jdweg 10 1quireq T Ke dsrp omodu uo TTI OpOW UR AA 216 HUT 3 UOA p1eoq 1o o guoo ooe dow p IO 9poul We A UI DST S1OOUSI AO Io o3juoo J t poq 1o o3uo p p1eoq 1o o juoo oq ose Aa HUEN Ap p1eoq ejdsrp sovjday e Sutssoid Aq DSZ Je soje n8o1 19 oyuoo wngu peog Aejdsiq p1eoq 1o o1juoo oq osje Kul Ajddns 1940d ae day z SoA EA prouo os ooejdos eT JOU J 10109002 Jomod piscoq 19 01UO9 18 OqAZI ZI GA c K ddns 140g z 100 JT ZN IJO mop Sutddojs S A 8A prouo os ULIJUOS Sur ooo p oo pys joquieqo mq opoui Ten eu ur jjo uo Daum o8e3 oA prouo os JI eq JOU J apo enu ui ur J9 onuoo Aq jjo uo poum oq UB9 OVAOEC OcT sprouo os IO 19jeoq uo oSe3 oA 9 3 7007452 CryoMed Freezer ulljuo somjzerodursy Teo puo oq aq o sieadde omje1oduio Teuuou s amp e dsirp p1eoq 1o o 3uoo ooe dow 1 JOQUIBYO pue 10 SuImoys e dsiGq jui I2A0 1opun 1 oqureuoO LT 19010 Ae dsrp ounje1oduro oN uonnjog UOVA osne o qIssoq mode opm sunoougso qno Y Thermo Scientific Jopiooai ooe dojgedow Lr TAD ptsur ssoureu 1op1ooo1 IO o qeo 1opioooi1oov dow GEI Jo o dureg 19quieu PAS 5 93IAS Iamod p1oo Jomod Ajddns jy 31091107 T Jop1ooo UE uorg1qi e uoreansuo up e poq 1o o3uo s 3uoso1d J Jop1ooo1 Jo opis uo sjsod A 6 xoidde ounseoaui pue O0 S 10 Iop10201 jos MUI Jop1ooos UE uo
7. Declaration of Conformity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 USA Product Description Controlled Rate Freezer Product Designations 7455 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC LVD 73 23 EEC This product conforms to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Declaration of Contormity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Milicreek Road Marietta Ohio 45750 U S A Product Description IVF Controlled Rate Freezer Product Designations 7457 Year of Initial Marking CE 2002 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC MDD 93 42 EEC This product conforms to the following Harmonized International and National Standards EMC MDD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 Richard L Miller COE Regulatory Compliance Man
8. Information Fields Data Tag Shows mandatory information entered by the user for the open Run file Date Time Shows the start time date of the open Run file Operator Shows user name or Anonymous if no users are set up in Options screen Review window status bar Shows the name and path of open Run file Review step select pull down menu Displays the steps of the profile in the open Review Run file Compare step select pull down menu Displays the steps of the profile in the open Compare Run file Thermo Scientific 7007452 CryoMed Freezer 10 19 Section 10 PC Interface Software R e VI ew S C r e e n Data Tag preset Date Time 12 20 2001 1 33 PM Operator Anonymous Sample LD sam122001 Cryoprotectant DMSO of samples 17 C 0 nt I n u e d Media Cel Culture Media Sample Size 2ml Time Division S minutes v t jii Chamber 25 9 Sample 22 1 Export review chamber Pa review sample User Controls Review Button This button opens an existing Run profile for on screen review and print out The Chamber and Sample temperatures can be examined over the time or the actual Chamber temperature may be compared with the programmed temperature Click the Review button again to open a different file Compare Button This button opens a second file for comparison Both files are plotted on the graph Click the Compare button again to remove the comparison data e Pr
9. Tp ae AN GRILL 370142 D SUB SCREW LUCK 1 SET RED e e 0527 WERT H SR BLOCK 1 SET TIE WRAP KY TIE ANCHUR AMP 5X20 MM FUSE Wiring Dio 2 Fs i582 03 08 02 RTT TT ARS ADU EXTERNAL IVE PROBE CONNECTION M in I F8 1592 02 21 02 RTT PDK LON TET MOTOR FUSE TU DRAWING 0 0 MA gio Ier TT RELEASED FOR PRODUCTION AS Y REV ECN NO DATE BY CAD Jarro DESCRIPTION OF REVISION 7450 100 1201 ER WODEL PART NAME CUNTRULLED RATE FREEZER nt 2 AND SUCH INFORMATION IS NOT TD Controlled Rote R OTHERS FOR ANY PURPOSE NX DIG TITLE WIRING DIAGRAM 100 AND 120 VOLT UNITS Freeze n MANLFACTLRING PURPOSE WITHOUT reezer WRITTEN PERMISSION FROM THERMO FORMA DWN PTT CAD RTT APPO LON JOATE 08 09 01 SCALE 0 167 MATERIAL 5 JP Th PAINT N A 7450 71 1 D REV 2 ermororma TOLERANCE UNLESS OTHERWISE SPECIFIED DRAWING NUMBER SIZE 4 5 Box 549 Marietta Ohio 45750 HEES DECIMAL X 7450 71 1 D age 2 z 5 e S75 Prs 7 l 7 16 7007452 CryoMed Freezer Thermo Scientific Section 7 Parts List G8 EXTERNAL SIMPLE PROBE CONECTOR IVE MODELS ONLY PART OF CABLE ASSEVELY 290179 20049 B 32 X3 8 SCREW 4 REN CONTROL PANEL 140296 191648 LED DISPLAY BOARD FRONT AND BOTTOM VIEW DETAIL 1 DOOR SWITCH CONNECTOR WIRING DOOR SWITCH CONNECTOR d FOR SERVICE REPLACEMENT DF CONNECT DIR S
10. 43 INA FRONT AN BOTTOM VIEW FIR SERVI SeA SWITCH N MAGNE D A x LR _ Ag 390027 IES 6 32 X 1 4 SEREN ARED 2 B J 23058 45 STAR LUCKWASHER i d CNET USE REFER TE DETAIL 1 DOUR SWLICH SERY N 320398 4 X 20 LOD DISPLAY FOR SERVICE ISPLAY I USE SIKE 400 59003 2 36 X 1 4 SCREW 4 REU LCD DISPLAY CABLE SOLDER CABLE TO LCD W CONNECTER POSITIONED AS SHOWN PRINTER CONNECTOR PART DF 185712 RS 232 CONNECTOR PART OF 183712 195 22 MAIN BOARD DC k 20006 HN WIRING 310558 I 5 EXTERNAL SAMPLE PROBE CONNECTOR IVF MODELS ONLY PART DF CABLE ASSEMBLY 290179 DETAIL 1 DOOR SWITCH CONNECTOR WIRING CUNNELTUR EMENT F L COMECT 0008 SWITCH V 370445 2 POS CONNECT A AND ALL 370351 STRAIN RELIEF DETAIL 2 120 VOLT MOTOR WIRING DETAIL 330003 3 8 SPLIT SNAP BLSHING DROWN WIRE 100 1204 PERATIEN tREENAREL WIRE WHITE WIRE 100 1204 PERATIEN 59007 4 40 X 3 8 SRE Da Wi n UW IA H Py 2 pF 1 RO EACH EW YS I m 2 QUAL INPUT SAMPLE PROBE SSS E Z C Wl TR iF MEELS INLY Cras d Y ho n 370249 SABLE THERMILILPLE PLUG d EN A l CHAMBER THERYOCOLPLE CONECTOR
11. 7007452 CryoMed Freezer 2 1 Section 2 Set Up and Operation Recommended Clearance all Models A 4 to 6 10 2 cm to 15 2 cm B 6 to 8 15 2 cm to 20 3 cm p 2 5 MINIMUM A gt 6 4 cm 000000 000000 000000 000000 000000 X 7456 43 5 110 5 cm 7457 43 5 110 5 cm X 49 5 125 7 cm 7458 49 5 125 7 cm 43 5 110 5 cm 7459 49 5 125 7 cm 43 5 110 5 cm 7475 43 5 110 5 cm 49 5 125 7 cm 49 5 125 7 cm 55 5 141 0 cm 55 5 141 0 cm 43 5 110 5 cm 49 5 125 7 cm 55 5 141 0 cm TOP VIEW Printer Cover d 25 63 5 cm Recommended Sample Holder Clearance d XX XXX 53 3 cm E e P y 23 2 F B w handle amp fittings 58 9 cm 21 SIDE VIEW NON IVF ONLY pou L TI ZEN VN Y 20 5 N 52 1 cm E Figure 2 2 Clearance for All Units SIDE VIEW IVF ONLY 2 2 7007452 CryoMed Freezer Thermo Scientific Thermo Scientific Supplies Electrical Connections Environmental Conditions Section 2 Set Up and Operation The Controlled Rate Freezer requires a clean low pressure 22 PSIG 30 LIQUID nitrogen supply Do not use the gas fitting on the supply tank A dedicated supply tank source is recommended for optimum operation The tank hose connection 1 2 flare fitting is located on the back of the unit in the bottom left corne
12. N T MO 00 2unssoud Apddns NT Z Ada guer A ddns NTT 1 wey ddnS NT 19940 uopnjos UOTJE2IJLI9 A 9sne o qissoq mode IPMA gunooqgso qno Y Thermo Scientific 7007452 CryoMed Freezer 9 2 p1eoq 1o o guoo ooe dow Lr jouSeui Jo pue yoyms 100p ooe dow 100P qojep eso 17 aqoid o dures ppe 10 o roud woy omyezxodura o dures o ourow eq sunoogse qnon IL 9S 1 pojuosoud som rej JI NUU oorAIoS uoneiqgi e uon en Suo UI S S9 um sou DIEMIJOS pue jsop uns p pueoq 1o o33u02 p J0U J sunqo 01 Ugy ss SI posojo JOOP UYA YIJMS JOOP Jo ooutjsiso1 uurguo youms Joog ugy ou mq 10 uny pouojej peso o AIT sr 100p wnguop c Jefesuado 100q Z omje1oduro o dureg osn jou soop o goud wayuod eq omje1oduio o dureg Jo 19quIego uo TTI I eqo1d o dureg 1equireq T aOd pojoo os UNI Jou ITAA p1eoq 1o o guoo ooe dow K ddns 1amod ooe dow z juoso1d jns Ke dstp T TL JI dijo 1aded 3uoq 10 our Jodum qj p1eoq 19 0 uo uo sindur o dnooouuq oys poq 1o o3uo JOU J rojo uuoo Jomod p1e0q 1e o3juoo Je OqAZI Z1 S A c K ddns 1am0g z 10 99uuoo sumo JO ssourey oqoid ooe do1 moj e uey o1ourj ordures JO JOQUIRBYO 10j 1eooprs ur 101090002 MATIS 10199003 SMOJOS 1O 99Uuuoo UJYYJIL ssourey oqoJd uo surd u 9A4 q ooutjsrso1 o1nseo A T
13. Single clicks scroll slowly or press and hold to scroll more quickly Press Enter to go to the next line in the profile 10 After all function commands are chosen press Back until the main menu appears Note After exiting Edit Mode the edited profile is printed on the thermal printer if printer is On and the selected output device See Section 3 A Note To edit a single line of a profile enter View Mode scroll to the desired profile step and go to Edit Mode by pressing Enter A Pre set and created user profiles can be copied then pasted into one of the ten User Profiles as templates to create a new User Profile To erase a User Profile already in use a blank User Profile may be copied then pasted over it Use the steps below to copy and paste 1 In the Main Menu select the Pre set or User Profile to be copied by using the up or down arrow keys 2 Press and hold approximately 3 seconds the Back key until the message Profile copied appears on the second line of the display 3 When the Back key is released the message Select dest with w appears on the third line of the display Select the destination for the copied profile using the up or down arrow keys When the desired User Profile is showing on the first line of the display press the Enter key to complete the paste operation or press Back to cancel Thermo Scientific Section 2 Set Up and Operation Recorder The recorder output connector on the back of
14. resolved Thermo Scientific 7007452 CryoMed Freezer 2 13 Section 2 Set Up and Operation 2 14 Sample Thermocouple Open continued Heater Malfunction 7007452 CryoMed Freezer If the Sample probe fails during Run Mode and the current profile does not use the Sample temperature as a trip point Alarm LED lights Audible alarm sounds continuously LCD Display shows S sensor fail on the fourth line Sample numeric display shows ILL Continue run normally Depressing the Silence key will silence the audible alarm and clear the Alarm LED and display messages The numeric display ILL will remain until the probe condition is resolved If the heater is energized continuously no active temperature control for 60 minutes the controller will sound a heater malfunction alarm light the Alarm LED sound the audible alarm continuously show the message Heater error on the fourth line of the display and immediately switch to Idle Mode and disable all functions The audible alarm is silenced by pressing the Silence key Since this alarm indicates a hard failure the Alarm LED and fourth line message can only be cleared by turning the unit power off then on again Thermo Scientific Chamber Sample Over Under Limit Temp Thermo Scientific Section 2 Set Up and Operation Caution Do not allow the sample probe to come into contact with the chamber interior during operation This could cause a d
15. 1 SET d LA us 31 1 39037 SMALL TIE WRAP Lecce c3 30081 STICKY TIE ANTS ARR TLE WIRING 2015 10 AMP 5X20 MM FUSE Tj SL VALVES 250004 L E SERVICE REPLACEMENT F VALVES USE STK 4000618 Wiring Diagram 2 FR 1582 03 08 02 RTT eT acs ADO EXTERNAL IVE CONNECTION 1 FR 1592 02 21 02 RTT POK LON ADDED MOTOR F DRAWING Model o NA azis ert ert BF RELEASED FOR PRODUCTION 7451 230V REV ECN NJ DATE BY can aepo DESCRIPTION OF REVISION THIS DOCUMENT CONTAINS PROPRIETARY MODEL PART NAME CONTROLLED RATE FREEZER EP e late INFORMATION AND SUCH INFORMATION IS NOT TI Control led Rave BE DISCLOSED TU OTHERS FUR ANY PURPOSE NOR DWG TITLE WIRING DIAGRAM 230 VOLT EXPORT UNIT USED FOR MANUFACTURING PURPOSE WITHOUT rreezer WRITTEN PERMISSION FROM THERMO FORMA OWN RIT CAD RIT APPO BH DATE 03 09 01 ALE 0 167 MATERIAL I Pur wa 1451 71 1 D REV 2 Thermo Forma TOLERANCE UNLESS OTHERWISE SPECIFIED DRAWING NUMBER SIZE Dann D 5 ANGLES DECIMAL XX oce 2 of 2 Box 549 Mariette Ohio 45750 Act 451 71 1 D 7 18 7007452 CryoMed Freezer Thermo Scientific POWER CONNECTION bk J PA f 5 120 VAC 60 HZ 12 i 2 450180 1 o o POWER ENTRY CONNECTOR AND EMI FILTER
16. 70 solution is volatile and flammable Use it only in a well ventilated area that is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard Allow the alcohol to fully dry before turning power on A Use an appropriate disinfectant All articles and surfaces to be disinfected must be thoroughly cleaned rinsed and roughdried 1 Turn the freezer off and disconnect the plug from the power source 2 Unplug and remove the Sample probe from the chamber clean with alcohol and allow to dry 3 Wash the cabinet interior with disinfectant starting at the top and working down The cabinet and door must be rinsed with sterile water until the disinfectant has been removed After the cabinet has been rinsed spray with 7096 alcohol 4 Allow the cabinet door to remain open for the alcohol to evaporate 5 Plug the freezer in and turn the power switch on 7007452 CryoMed Freezer 4 1 Section 4 Maintenance Clean a Major Spill in the 1 Turn the unit off and disconnect it from the power source Chamber Warning Be sure to disconnect the power source as the interior fan may start unexpectedly under certain conditions A 2 Disconnect the probe from the connector if applicable Remove the sample holders IVF only 3 Remove the two screws at the top and bottom of each side securing the screened side ducts to the chamber Set the ducts aside 4 Press the top
17. 84 40 SS HEX NUT l D 23021 8 SS FLAT WASHER 4 7 23058 8 SS EXT TOOTH LOCKWASHER 2 8 23082 10 SS SPRING LOCKWASHER 4 9 23084 4 SS SPRING LOCKWASHER 4 0 30092 3 16 SHORTY SNAP BUSHING l 00115 O CW FAN BLADE 40 PITCH 2 00117 MOTOR FACE MOUNTING PLATE GASKET 3 11002 3 8 PROBE CLIP 4 11022 74 DIA BRASS FUSE CLIP 2 5 27075 48 32 X 813 LONG M F STANDOFF 6 37017 9845 WATT 115V TUBULAR HEATER 7 56123 74 HP BLOWER MOTOR 1725 RPM 8 80217 NJECT LINE CDLLAR g 95715 FAN BLADE MOTOR HUB 20 290170 CRF T TYPE CHAMBER PRDBE 21 290171 CRF T TYPE EXTENSIUN CABLE 22 370248 THERMOCOUPLE PLUG TYPE T 23 430240 FLAT BRAID GROUND STRAP 24 510033 810 32 X 3 8 SS HH MACH SCREW 4 25 515084 1 4 DIA X 3 8L SS SPACER 4 26 700036 1 4 28 X 3 8 LG S S HEX SOCKET SET SCREW 2 27 730058 11 GA SS 2B FLAT WASHER 4 28 73007 450 ID TEFLON FLAT WASHER 28 1880008 SAMPLE PROBE CONNECTOR MOUNT 30 1890009 SHAFT SEAL PLATE 3l 1890012 MOTOR MOUNTING PLATE 32 1890040 LN2 INJECT COIL SUB ASSEMBLY 34 137018 945 WATT 230V TUBULAR HEATER EXPORT ONLY 7 8 7007452 CryoMed Freezer CABINET ASSEMBLY 7452 201 2 B REV 2 PAGE 2 DF 2 Thermo Scientific Section 7 Parts List THESE PARTS USED FOR EXPORT MODELS ONLY PART DF ITEM 18 mz g Pe PART DF ITEM 428 DC POWER CORD INCLUDED WITH POWER SUPPLY BUT NOT SHOWN SEE DRAWING 7450 71 1 FOR ADDITIONAL COMPONENT AND WIRING DETAI
18. Calibrate A Change Access Code Change the four digit security access code by selecting each digit using the Enter and Back keys and changing the digit using the up down arrow keys The default Access Code security disabled is 0 0 0 0 Each digit may be set over a range of 0 to 9 Exit this menu by using the Back key 7007452 CryoMed Freezer 3 1 Section 3 Configuration Calibration 3 2 Menu Entry Definitions cont 7007452 CryoMed Freezer B Chart Recorder Selects the chart recorder operating parameters and checks calibration Exit this menu by using the Back key a Select Chart Recorder Output Select chamber only sample only chamber sample scan 2 sec chamber 10 sec sample or no chart recorder function Off The default setting is Off b Calibration Linearity Verification Verify chart recorder calibration and linearity by setting the chart recorder function over a range of 180 C OVDC to 50 C approximately 10VDC in 10 steps Note The chart recorder function matches the non linear scale on the chart paper P N 197044 A Optional Flatbed Recorder Calibration Procedure l Press the power switch on the flatbed recorder and allow the system to warm up for 20 minutes Uncap and lower the pen to the graph paper From the freezer Configure calibrate menu select Chart Recorder Turn the chart recorder on to Scan mode and press the Enter key Set the temperature to 0 C zero IMPORTAN
19. Chamber 90 0 C Figure 10 2 Freezing Profile On the Edit screen a freezing profile can be created and saved by the operator An existing profile may be edited if required A profile listing can be printed An approximate representation of the freezing profile will be displayed in the Edit window graph These are the profile steps available in the pull down menu with definitions following Step Name with Menu Format Step Parameters with Definitions Wait Wait At X X C where X X is the chamber set temperature Maintains specified chamber temperature indefinitely until the user clicks the Program waiting for user Click here to continue button on the Run screen The temperature can be set over a range of 180 C to 50 C with a setting resolution of 0 1 C Thermo Scientific 7007452 CryoMed Freezer 10 3 Section 10 PC Interface Software Edit Screen Fa conti n ued Command Wait for Temperature m Chamber iE Rep Sample 5 0 KE Figure 10 3 Wait for Temp Step Name with Menu Format Step Parameters with Definitions Wait for temperature WAIT AT CHAMBER X X C UNTIL SAMPLE Y Y C where X X is Chamber set temp and Y Y is the Sample trip temp The default Sample trip temp is1 C above Chamber temp Step maintains specified Chamber temp and moves to next step in profile when Sample temp cools to the specified temp This step can only be used when the starting Sample temp is greater than the sp
20. Chamber Sensor Failure Sample Sensor Failure Chamber Limit Temperature Sample Limit Temperature Heater Malfunction LN Supply Run Complete Warm Complete CREF Printer Buffer Full When an alarm condition occurs sudible and visual alarms are presented at the freezer and an alarm window appears on the PC screen The window contains a list of up to three of the most recent alarms and the message Click Here To Silence Click anywhere on the alarm window to silence the audible alarm at the freezer After the audible alarm is silenced another alarm window appears showing the list of up to three most recent alarm and the message Click Here To Clear Click anywhere on the alarm window to clear all alarms whose conditions have been resolved The alarm window reappears if any alarms are still active Correct these conditions and click anywhere on the alarm window to clear these alarms If the message Profile Stopped At Freezer displays the run has been stopped by a component failure in the freezer or by the user pressing the Back button at the freezer while the profile is in the End step Click on the message to acknowledge that the run was stopped This action has the same effect as clicking the Stop button Thermo Scientific Thermo Scientific Typical Run Procedure Section 10 PC Interface Software Click the Run button on the Main screen Select the PC commmunication port Avail
21. Repl Tire 5 minutes Figure 10 5 Hold at Temperature Step Name with Menu Format Step Parameters with Definitions Hold HOLD X X C FOR Y Y MINUTES where X X is the chamber set temperature and Y Y is the minutes Maintains a specified Chamber temperature for the specified hold duration before proceeding to the next step The time can be set over a range of 1 to 99 minutes and the temperature can be set over a range of 180 C to 50 C with a setting resolution of 0 1 C Command Jump Y Step C Repl Figure 10 6 Jump to Specified Step Jump JUMP TO STEP X where X is the step number Automatic advance to a subsequent step or return to a previous step in a freezing profile The Jump function can be programmed over a range of Step 1 to Step 20 Thermo Scientific Edit Screen continued Create a New Freezer Thermo Scientific Profile Section 10 PC Interface Software GE Command Loop M Step Rep Loops 1 Figure 10 7 Repetitions From Specified Step Step Name with Menu Format Step Parameters with Definitions Loop LOOP X TIMES STARTING AT STEP Y where X is number of repetitions and Y is the step number to begin Automatic advance to a subsequent step or return to a previous step in a freezing profile for a programmable number of repetitions The Loop function can be programmed over a range of Step 1 to Step 20 and over a range of repetitions from 1 to 99 End
22. SCREW 4 J 23080 8 SS SPRING LDCKWASHER 4 6 103113 SILICONE D GASKET 7 117018 7716 DIA BLACK HOLE PLUG 2 8 121065 VICE ACTION T HANDLE LATCH H 180223 1 2CF CRF DOOR HEAT BREAK FRAME 0 190612 ALUMINUM HINGE BLOCK 2 l 360235 SURFACE MOUNT MAGNETIC SWITCH MAGNET 2 515084 1 4 DIA X 3 8L_SS SPACER 2 3 670015 YLUN PUSH IN RIVET 29 4 890002 DDDR ASSEMBLY S 880018 DOOR INNER PAN SUB ASSEMBLY 6 890020 SECONDARY SEAL RETAINER FRAME 7 180222 O 6CF CRF DOOR HEAT BREAK FRAME 8 880034 0 PE DOOR ASSEMBLY 9 880036 0 6CF DOOR INNER PAN SUB ASSEMBLY 20 880038 10 6CF SECONDARY SEAL RETAINER FRAME 2l 180224 1 7CF CRF DOOR HEAT BREAK FRAME 22 880043 1 7CF DOOR ASSEMBLY 23 890045 1 7CF DOOR INNER PAN SUB ASSEMBLY 24 880047 1 7CF SECONDARY SEAL RETAINER FRAME n 7007452 CryoMed Freezer R ASSEMBLY 7452 200 1 B REV PAGE 2 UF 2 ur SUME FASTENER QUANTITIES WILL VARY BASED ON DIFFERENT SIZE UNITS l Thermo Scientific MOUNTS TO CEILING DF INTERIOR CHAMBER MOUNTS TD REAR WALL SE DF INTERIOR CHAMBER m SN gt MOUNTS TO CEILING MOUNTS TO FLOOR OF OF INTERIOR CHAMBER MOUNTS TO FLOOR OF INTERIOR CHAMBER INTERIOR CHAMBER Section 7 Parts List CA BIN BLY 7452 201 EV 3 PAG N As Thermo Scientific 7007452 CryoMed Freezer 7 5 Section 7 Parts
23. a warm tube is inserted into liquid nitrogen liquid will spout from the top of the tube due to gasification and rapid expansion of liquid inside the tube Warning Nitrogen gas can cause suffocation without warning A Store and use liquid nitrogen only in a well ventilated place As the liquid evaporates the resulting gas tends to displace the normal air from the area In closed areas excessive amounts of nitrogen gas reduce the concentration of oxygen and can result in asphyxiation Because nitrogen gas is colorless odorless and tasteless it cannot be detected by the human senses and will be breathed as if it were air Breathing an atmosphere that contains less than 1896 oxygen can cause dizziness and quickly result in unconsciousness and death Note The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture not the gas itself The issuing gas is invisible A Never dispose of liquid nitrogen in confined areas or places where others may enter Disposal of liquid nitrogen should be done outdoors in a safe place Pour the liquid slowly on gravel or bare earth where it can evaporate without causing damage Do not pour the liquid on pavement If a person seems to become dizzy or loses consciousness while working with liquid nitrogen move to a well ventilated area immediately If breathing has stopped apply artificial respiration If breathing is difficult give oxygen Call a physician Keep warm and at rest
24. adding or editing a profile Note If Access Code protection has been enabled the unit will prompt for the code before entering Edit Mode To enable an access code see Access Code Security in this section A If the profile list is not displayed press the Back key as many times as is necessary to view them 1 Locate the arrow to the left of the option that is on the top line of the display Move the profile USER 1 through USER 10 that you wish to view or edit to the top line by pressing the 5or 6 keys Note Pre set Profiles may not be edited A 2 To enter View Mode press the Enter key 7007452 CryoMed Freezer 2 7 Section 2 Set Up and Operation Program User Specific Profiles cont 2 8 7007452 CryoMed Freezer 2 4 Press the up arrow key to view Step 0 This is the Name Title step Press Enter to go into Edit Mode for Step 0 and the cursor will be blinking over the leftmost character position To change this character scroll through the available characters by pressing the up or down arrow keys Press Enter when the chosen character is visible The cursor will blink over the next character in the name or title The available characters and their relative sequence is as follows H 8 _ Othru9 lt gt 0 AthruZ _ athruz gt Sp To delete a character place the cursor over the chosen character and D press Silence Note When starting with a space Sp pressing the up arrow key gives y
25. and bottom edges of the center duct toward each other and draw the duct outward to remove 5 The chamber is now fully accessible and can be cleaned 6 Clean the interior of the unit with an appropriate disinfectant Clean rinse and wipe dry as needed Clean the screened side ducts and center ducts before reinstalling 7 Press the top and bottom edges of the center duct toward each other and insert the duct into the center of the chamber The edges fit inside the door gasket 8 Install each screened side duct Locate the tabs on the back of each duct into the proper holes at the back of the chamber Align the two screw holes and secure at the top and bottom of each side Caution Be sure all chamber components are re installed in the proper locations to avoid compromising the chamber airflow and temperature uniformity A Caution Avoid damaging the chamber opening gasket A 9 Connect the Sample probe to the connector if applicable 10 All chamber parts must be re installed for consistent uniformity and airflow Connect the unit to the power source and turn it on 11 The freezer is ready to be returned to service 42 7007452 CryoMed Freezer Thermo Scientific Clean the Cabinet Exterior Clean Gasket Heat Thermo Scientific Break Section 4 Maintenance Clean the freezer exterior with a damp sponge or soft well wrung cloth and mild detergent dissolved in water Dry with a soft cloth Note On IVF models be sure t
26. assurance that the PC user will be aware of the alarm condition The other freezer keypad keys are disabled to prevent persons near the freezer from taking control of a freezing run without the knowledge of the user initiator of the run All freezer status and temperature displays operate normally The message Under Remote Control displays on the freezer as well as the current profile step being executed Alarm messages alternate with the current step information if an alarm condition occurs Thermo Scientific Section 10 PC Interface Software Stand Alone Mode The PC can only capture time temperature and alarm data in this mode No action commands are permitted Current operating status and chamber sample temperatures will be updated and displayed normally The freezer keypad is still disabled in this mode except for the Silence key However a run may be aborted by pressing and holding the Back key on the freezer for 3 seconds All freezer status and temperature displays operate normally The message Running Remote Profile is displayed at the freezer as well as the current step information alternated with any alarm messages Running a Local Profile An attempt to open a Run screen for a freezer already running a local profile will result in an error message Freezer Already In Use The Run screen cannot be opened Review Screen Refer to the details below concerning the information fields and user controls for the review screen
27. box corresponding to the desired plot item Move the cursor over the desired color observe the changes in the sample graph Click the palette button to create custom colors When the cursor is over the desired color click the left mouse button Click Save to keep changes Click Restore to return to previously saved colors Click Exit to exit Options menu TFS Configuration Options Plot Colors Information Fields Default Directories Users Select the colors to be used for the graph displays The graph displays in the Edit Run and Review modules V gt Restore all use these colors Be aware that for color printouts of any graph the background is always set to white Therefore any light colors used may be harder to see on color printouts Exit Chamber plot CO Sample plot E Profile plot El Difference plot WI Alarm markers s Step markers E Background E LI Grid Thermo Scientific 7007452 CryoMed Freezer 10 23 Section 10 PC Interface Software Information Field Set Up 10 24 Default Directories 7007452 CryoMed Freezer Select the number of information fields to be displayed on the Run screen Information field names and contents are saved with the Run data file for each enabled field Be sure to save choices before leaving the Information Fields screen Click the check box next to a field to enable it Enter a name for each enabled information field Click Save to keep the changes Click Restore to r
28. cycle the controller will sound a Warm Complete alarm Flash the Alarm LED and sound an audible tone at a 2596 On duty cycle 2 second period Show Warm Complete on the fourth line of the display Chamber temperature is maintained at 25 C for a period of 30 minutes then switched to Idle Mode and all functions are disabled When the Hold time period has elapsed two audible tones sound to notify the user of the completion of the Hold step and the continuation of the profile run This can be used to alert the user that a particular point has been reached in the profile When seeding be sure to use the top access ports rather than the door This alarm message appears when the printer storage buffer is full of Run data approximately 2 hours and is no longer able to store data for later printing Press Silence to mute the alarm However the LED will continue to flash until the Run is stopped Press Silence to clear the alarm message Between the time the Printer Buffer Full messsage displays and the user stops the Run no data is captured The printer buffer will store the previously captured data until the next Run is started The buffer is cleared at the start of each new Run Note The printer continues to print current Run data even though the Printer Buffer Full alarm has occurred Included with the IVF Controlled Rate Freezer are a straw holder vial holder cane holder and access port plugs The
29. itself The issuing gas is invisible In closed areas excessive amounts of nitrogen gas reduces the concentration of oxygen and can result in asphyxiation Because nitrogen gas is colorless odorless and tasteless it cannot be detected by the human senses Breathing an atmosphere that contains less than 19 5 oxygen can cause dizziness and quickly result in unconsciousness and death Therefore the use of oxygen monitoring equipment is strongly recommended A Warning Contact of liquid nitrogen with the skin or eyes may cause serious freezing injury A This freezer should be located in a well ventilated area of the laboratory on a table bench capable of supporting the unit s weight see Specifications section with adequate workspace available Allow clearance for the open door on the front of the unit for the hose and power connections on the back and the exhaust vent on the left side Do NOT attach anything to or tamper with the exhaust vent Locate the unit in close proximity to the LN source Additional space for a On Off Switch temperature recording device mains may also be desired The disconnect RS232 Interface connector for a recorder is Power Strip Chart located on the back of the unit Inlet Recorder Interface next to the power connection Tank Hose When closing the freezer door Connection be sure to turn the handle 180 in rotation to fully seal the door Figure 2 1 Connections on Unit Back
30. j GRN YEL 2 gt O 3 u _ SNITCH CIRCUIT BREAKER 230184 15 AM 2 PRINTER POWER OUTLET 4 xe 5 460024 e M PRINIER PUWER SUPPLIES gt 201185 Q ry PRINTER G 201183 2 CONTROL PANEL 4 DISPLAY BOARD 191648 D LCD G lt LED JI LCD J3 2 e 8 PC PRINTER EXTERNAL PE CUNNECTIDN 9 m MAGNETIC DOOR SWITCH J gt e 360235 ES oL RS s 1 A 20 37 36 a SHOWN DOOR OPEN Le 8 gt Uer en ra K 0 33 2VOC COOLING FAN 22 900134 8 VDC IN 23 31 MAIN BOARD 1916 ANALOG y 24 RECORDER S OUTPUT a E al ANALOG OUT Egal J10 200178 IVF EXTERNAL SAMPLE PROBE CONNECTOR ASSY TVE SAMPLE T COUPLE 21000402 R 4000403 1 Le WE MODELS IN Y lt 290171 EXTENSION _ SAMPLE T COUPLE 294003855 lt CHAMBER T COLPLE soio 205 LE 230195 30200170 T 10A MOTOR FUSE ESCH ABI rires pi J12 j2 COULE COOL MOTOR HEAT el QS SOS laa sy r d l E i 33 ite a 7 Thermo Scientific Y RED 17 RED 18 RED 20 REO 21 EATER 1028 WATTS AT 12 37017 OVERTEMP T STAT 400148 INTERNAL CIRCULATION FAN 56116 OR 156123 WIRED FOR 120 VOLTS COOL VALVE 250093 COOL PLUS VALVE 250093 Electrical Schenotic Model 0 7452 7454 7456 and 7458 Control Rate Freezer 7450 70 1 D REV 5 Page of 2 7007452 CryoMed Freezer 8 1
31. qualified service personnel A Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual 2001 Thermo Scientific All rights reserved 7007452 CryoMed Freezer Thermo Scientific Preface Important operating and or maintenance instructions Read the accompanying text carefully Potential electrical hazards Only qualified persons should perform procedures associated with this symbol Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed
32. registered an alert screen will display on start up The registration passcode is T2YRPW9PWEXD28GJ When installation is complete click the Option button to set default directories information fields and add user names and passwords if password protection of all Main Menu features is desired System software Lab View Run Time Engine is installed along with the PC interface software Note This system software is required for proper operation of the PC interface with the freezer A Main Menu Ax the PC interface program be ins a splash screen Figure 10 1 appears Pros 8 P 8 PP just before the registration screen The splash screen contains the company logo contact information and a picture of the Controlled Rate Freezer The Main Menu buttons are displayed Edit Run Review Options and About Selected button turns dark when activated e The About screen displays the Thermo family of laboratory equipment plus the company Mission statement Click anywhere on this screen to close it The Exit button closes the PC interface application software All other windows must be closed before exiting to avoid error messages 10 2 7007452 CryoMed Freezer Thermo Scientific Section 10 PC Interface Software Edit Screen 2 Ramp 1 0 C min until Sample 5 0 C 3 Ramp 20 0 C min until Chamber 40 0 C 4 Ramp 10 0 C min until Chamber 10 0 C 5 Ramp 1 0 C min until Chamber 35 0 C 6 Ramp 10 0 C min until
33. skin packets resulting in a 1 C rate from nucleation to 35 C and a 10 C per minute cooling rate to a 90 C end temperature Step 1 exo diated ayes D e ud ep diis Wait EE 1 0 C m S EDO A ras 20 0 C m C EE A M t AAA i 10 0 C m C Step usa cis es MEN a 1 0 C m C Step O iuvare kae dr xad eS 10 0 C m C EA ore uetus eae vs by de vm eate End at 4 0C to 5 0C to 40C to 10 0C to 35C to 90C 7007452 CryoMed Freezer 1 5 Section 1 Introduction 1 6 Pre set Profiles continued 7007452 CryoMed Freezer Pre Set Profile 46 Commonly used for hemopoietic stem cells that are 65 100ml in size resulting in a 0 5 C rate from nucleation to 20 C and a 1 C per minute cooling rate to a 80 C end temperature A dni oe rt eti etas Wait Rep 2 1 0 C m S SED OM etie a wu e due 20 0 C m C ele 10 0 C m C Step D ga ad eqq quien waa 0 5 C m C SEDO qi doe EX tae eee does 1 0 C m S EE End S Sample Temperature C lt Chamber Temperature C m degrees Centigrade per Minute at 4 0C to 0C to 45C to 10 0C to 20C to 80C Thermo Scientific Thermo Scientific Section2 Set Up and Operation Warning Nitrogen gas can cause suffocation without warning Store and use liquid nitrogen only in a well ventilated place As the liquid evaporates the resulting gas displaces the normal air in the area The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture not the gas
34. supply 2 Pollution Degree describes the amount of conductive pollution present in the operating environment Pollution Degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation 7007452 CryoMed Freezer 2 3 Section 2 Set Up and Operation Sample Thermocouple Connection Power Up 2 4 7007452 CryoMed Freezer Non IVF models Open the unit door 2 Locate the thermocouple connector in the front upper right corner of the chamber 3 Install the supplied sample thermocouple connector in the correct orientation 4 Close the unit door IVF models 1 Locate the thermocouple connection on the top of the unit to the left of the printer cover refer to Figure 1 1 2 Install the appropriate supplied sample thermocouple in the correct orientation Note The freezer has been calibrated with the internal thermocouple connector Refer to Section 3 for probe calibration A Turn the freezer power switch on the back of the unit to On On the control panel the Alarm indicator lights briefly and the LCD display reads Controlled Rate Freezer XX XX software version The Idle indicator lights then the LCD display shows the profile list To choose a profile scroll through the selections using the up down arrow keys The profiles are displayed in a loop Pressing either of the arrow keys consecutively brings the list back to the s
35. the Controlled Rate Freezer provides an analog signal representing process temperature s that can be plotted on an optional strip chart recorder The controller can be set to output Chamber or Sample temperature a scanned combination of both or Off Refer to Section 3 Note The chart recorder function is calibrated to match the non linear scale on the chart recorder paper P N 197044 A Printer The printer on the top of the Controlled Rate Freezer provides a visual record of a profile run including any errors which might have occurred Run data is captured in a printer buffer for later reprinting even if the printer is not enabled during the Run The printer buffer is cleared at the start of each new run It is recommended that a supply of printer paper be kept on hand If paper should run out during a freezing run first install the paper Then when the Run is complete the print out may be re started Useri 1 Wait at BD 4 7 2 End Typical profile listing after an edit DATE Dec 10 2001 TIME 2 46 17 pm lt Run start time and date Use 1 m Profile name SAMPLE 1 D 1234 MEDIA cell culture media wasa a 2 DMS0 Tat formation AMOUNT 2mL NUMBER OF SAMPLES 17 Time Temp scale Chamber temp NOTE Each horizontal line spacing equals one minute Sample temp 1000 80 60 Kid 20 po m 4 Profile steps listed as they occur Temp tracking alarm w C Prfile line F
36. the entrance opening of any liquid nitrogen refrigerator or dewar Do not use any stopper or other device that would interfere with venting of gas These cryogenic liquid containers are generally designed to operate with little or no internal pressure Inadequate venting can result in excessive gas pressure which could damage or burst the container Use only the loose fitting necktube core supplied or one of the approved accessories for closing the necktube Check the unit periodically to be sure that venting is not restricted by accumulated ice or frost Use proper transfer equipment Use a phase separator or special filling funnel to prevent splashing and spilling when transferring liquid nitrogen into or from a dewar or refrigerator The top of the funnel should be partly covered to reduce splashing Use only small easily handled dewars for pouring liquid For the larger heavier containers use a cryogenic liquid withdrawal device to transfer liquid from one container to another Be sure to follow instructions supplied with the withdrawal device When liquid cylinders or other large storage con tainers are used for filling follow the instructions supplied with those units and their accessories Do not overfill containers Filling above the bottom of the necktube or specified maximum level can result in overflow and spillage of liquid when the necktube core or cover is placed in the open ing Never use hollow rods or tubes as dipsticks When
37. 0 minutes after the Warm Complete alarm occurs If the Controlled Rate Freezer experiences a power failure and the PC continues to operate When power is restored the freezer will continue the Run at the point of failure However the freezer runs in Stand alone mode All commands from the PC are ignored except for profile status requests The temperature data is continually captured and plotted The PC cannot clear alarms or stop the Run The freezer s keypad remains disabled except for the Silence key which may be used to mute or clear any alarms The Run can be aborted at the freezer by pressing and holding the Back key for 3 seconds The PC will then resume control of the freezer Pressing the Stop button on the Run screen ends data capture only The PC will report a Power Fail alarm including the failure duration if the failure duration was longer than 10 seconds The graph on the Run screen will show a temperature occurrence of zero to indicate a freezer power failure rather than a PC only power failure A communication error message will display on the Run screen during the freezer power failure Thermo Scientific Power Failure Recovery continued Error Messages Thermo Scientific Section 10 PC Interface Software If the PC experiences a power failure and the Controlled Rate Freezer continues to operate The freezer switches to Stand alone mode if there is no communication from the PC for more than 20 second
38. 0170 J8 IE g TBI 2 32 mi COOL MOTOR HEAT e 00000000 joo 34 WA cf E eh Lo PE 1 EAT Electrical Schenotic 33 HEATERS ats 3 Soe 2 OVERTEMP T STAT Model m P Za n 400148 Gr GC TOME f ei 3 VE INTERNAL CIRCULATION FAN 1472 7473 7474 1561 7475 ond 7476 P zi WIRED FOR 100 VOLTS 143 0NQ 4 0 GH 3 Ur CO I IVP CR Freezer 3g RED n Sa olf COOL VALVE e RED B V 250093 AR I VENE RED 20 COOL PLUS VALVE 472 70 1 D REV 2 250093 BE Suu a Page of 2 Thermo Scientific 7007452 CryoMed Freezer 8 5 8 6 I ud ow WwW Cc WIRE 4 CO 1 CD CHI P Lil ST J EE ly AS OU SES RO NJ RO RO EXTE EXTE FEMA PIN IN PIN ANAL FEM PIN PIN SNAL RNAL LE S 2 TR 3 RE 5 SI UG R b AUGE RIRI Em E LE ELE NJ Pe Be mm E c 0 NN Mm oO fi CX3 EI OY Gi Ga 00 Ga OB EC Dr BM NS EI lA S LS NECTION INIATU MIT DA VE DA L I zu GROUND P CORDER UUPU KE wu EN CT CD UJ ETT Lob wu ET D Z HS TT Y ES did T ZO T EL ELL T wi LE E et Zu vtr Tl E NA E e Mam T NAY rm T CONNECT ION CONNECTOR 0 10VDC NONLINEAR BMINTATURE D CONNECTOR AED SN ATTENTION OBSERVE PRECAUTIONS ELECTRUSTATIC SENSITIVE DEVICES 2 FR 1582 03 08
39. 02 RTT POK AKS ADD IVF PROBE CONNECTIONS SI B280 6 19 01 HIT RIT 8H CHANGED WATTAGE FROM 1000 N A 15 29 01 RTT RTT BH RELEASED FOR PRODUCTION REV ECN NO DATE BY CAD APPD DESCRIPTION OF REVISION HIS DOCUMENT CONTAINS PROPRIETARY WUUELAARI NAME CENT RATE FREEZER 7472 73 74 75 k 76 INFORMATION ANO SUCH INFORMATION IS NOT TD BE DISCLOSEO TO OTHERS FOR ANY PURPOSE NOR ISEO FOR MANUFACTURING PURPOSES WITHOLI WRITTEN PERMISSION FROM THERMO FORMA Thermo Forma BOX 549 MARIETTA OHIO 45750 DWG TITLE ELECTRICAL SCHEMATIC Dm RIT CAD RIT ween BH DATE 5 29 0 SCALE NONE MATERIAL PAINT COLOR DRAWING NUMSER SIZE 7472 70 1 D Electrical Schenotic Model 7472 7473 7474 475 and 7476 IVF CR Freezer 7472 70 1 D REV 2 Page 2 of 2 7007452 CryoMed Freezer Thermo Scientific Joyeay ooe dow e pieoq Jo o guoo ooe dos qI jnojno Team ooe dow eq jnojno peuo Joso T 9SLI omodu Joquieqo ou pue uo 1923y JIM opour Ten ur 191690 SSOINV QV ALOZ 06 Vey o1our ounseojq c Ho ou mq UO JI opouir enue ur JJO UO 22610 191820 oumseoui qu s id sSo oun rej 1osuos JO yW 1940 JT QT ouou g suie e 9M IB JOSUOS 1o pue o1je1oduioj WU JOAO JOQUIBYO 107 s307 Jol ouruex4 eq momo SUSU yooyD 1 lopoH Z preoq 1o oyuo qq momy pud 19H 1 wey JOLY 19 eoH poq 1o o guoo ooe dow p p1eoq 1o o guoo IQ os e Aegu Ke dstp 197 P
40. 1 D Page 2 of 2 8 2 7007452 CryoMed Freezer Thermo Scientific Thermo Scientific POWER CHNNECTTEN 8 AA 4 0 90 250VAC INPUT PRINTER l 5 12 12VOC OUTPUT 201183 SWITCHING PONER SUPPLY 400119 CONTROL PANEL DISPLAY BDARO 91648 L Eech 18 LS un rg EXTERNAL PC ES 19 ONNECTION yia di B s DOR sw Sa 20 J J D 7 ki CH ys 2 LE Dar a G N 4 8 n 12400 COOLING FAN 22 900134 23 e MAIN BOARD 91649 I ANALOG e 24 __ RECORDER OUTPUT can ANA U P 25 J10 26290179 VF EXTERNAL SAMPLE V PROBE CONNECTOR It SAMPLE T CDUP 2 4000402 OR 4000403 lt IVF MODELS ONLY 28 290171 EXTENSION OO SAMPLE T COUPLE_ 4000385 m H 230185 ER COUPLE 10A MOTOR FUSE 2 y 280170 lt B Iu j 1 32 DU CDUL TR HEAT Ia H OMN AO vec 39 HEATER 1 So 12 3 r ge EET 36 8 eli 37 D ecd 5 38 m 1 Aye 8D n Neg 2 22 RED 2 oo RD 21 230 VAL 50 60 HZ 5 5 AMPS n o 2 VU 3 ave CN e 2 de OF 4 5 4 j 450180 POWER ENTRY CONNECTOR ANU EMI FILTER SWITCH CIRCUIT BREAKER 230178 8 AMP PRINTER POWER OUTLET 460105 IC DOOR SWITCH SHOWN DOOR OPEN HEATER 945 WATTS AT 230 Y 37018 DVERTEMP T STA 400148 INTERNAL CIRCULATION FAN 56123 WIRED FO
41. 1901 o Bunejas sobewep uoneyu oyjm Bulpnjour saBewep jenuenbesuoo Jo joaJpul Aue 104 ajqel aq jou eys oway AlddV TIVHS 3SOdYNd dg ITU gd V YO SSANLIA YO ALMIGVLNVHOYAW JO S3ILNVSHSHVM ON O211Idu YO 1V3O NILLIVM Y3HLIHM SSILNVYYVM Y3HLO TIV JO nain NI ANY 3AISNT19X3 SI ALNVYYVM SIHL uoneunsep GO 4 peddius se sued jueujeoe daJ pue pied aBejsod OUJJ9U o peuunjaJ aq 1snuJ syed An5 J p je uondo s ouueu jw 3ueuudinbe Jo sjueuoduioo Aug jo Una 104 enodde Joud aD snui jueuniedeq seorues eoiuuo9 SUL poned jueJem euiBiuo ayy puo Keq ped 1ueuoduioo y oj Jo juaudinba au Jayya o uenem y puajxe jou eys AjueueM siu Jepun jueuudinbe Jo sued sjuauodulo9 jo ed Jo jueujeoej des QUE IPA SIU wo pepnjoxe eJe sjejuud jafyul pue siolluouu sjejnduuoo euosi d sjeyseb siay sse B sway ejqepuedx3 sited 1 Aue jo eoueuuojed oj soud uonoaeup pue uoneuiuJejep AUBeJJe JO pejoejuoo eq 1snuJ jueunjedeq seal es jediuyos aU lL juewealbe jueJem siy Aq paJaAoo jou ae uoneJqieo pue uonejejsu pepnjoul Joqe esuedxe s ouuau 1e peoejdaiJ Jo peJiedaJ aq Wu diysuewyJom JO s euiejeuu ui Buruuojuoo uou eq 0 UBAOJd sued jueuoduuoo 169A jem ayy Buung poued AjueueM Jes 3814 ayy Buunp aumo juenbesqns Aue o spuajxa uonoejoud jueJem SU peJeAjep s jueuudinbe noA euin sues y jejeuixoldde je 15 JJ ou oB Wu jueJem ay os au Buiddius 104 Swoj e siu A roej ino wouy peddius si jueuudinboe JNOA ejep y uo Saa OM SUE
42. 2 01 SCALE NONE ontr ol Ro ve rreezer VEN ELECTROSTATIC MATERIAL T o o 4 ATI a 4 B ZZ SENSITIVE DEVICES ETT 7451 70 1 D REV 2 Thermo Forma DRAWING NUMBER SIZE es A G BOX 649 MARIETTA OHIO 45750 7451 70 1 D Q Q edo f 8 4 7007452 CryoMed Freezer Thermo Scientific POWER CONNECTION Ca Iii AA MY 100 VAC 50 60 HZ 10 5 AMPS 7 460180 1 POWER ENTRY CONNECTOR AND EMI FILTER 3 GRN YE 2 m 3 SWITCH CIRCUIT BREAKER 230184 15 AMP c n l l 9 Tul qul PRINTER POWER OUTLET 4 cae 5 460024 5 PRINTER POWER SUPPLIES 7 i 201186 8 i 5 12 12V0C OUTPUT m SWITCHING PUWER SUPPLY y PRINTER U 400152 SS 2 CONTROL PANEL 4 c DISPLAY BOARD 3 191648 D 7 I 7 KEYPAD f LED Jl LCD J3 p EXTERNAL PC CONNECTION g TA MAGNETIC DOOR SWITCH Lal 11 pea 360235 20 7 B JUR GE SHOWN DOOR OPEN en 9 N CHV 5 2 4 b e gege E 12VOC COOLING FAN DN 22 900134 S i ACND VOC IN 23 ES F H MAIN BOARD 191649 ANALOG 24 __ RECORDER E OUTPUT ANALOG DI PAS d 310 7280178 IVF EXTERNAL SAMPLE CO PROBE CONNECTOR ASSY Es TVE SAMPLE T COUPLE 2 4000402 DR 4000403 x IVP MODELS ONLY 290171 EXTENSION QO SAMPLE T COUP 4000385 lt 30 230195 V CHAMBER T COU 10A MOTOR FUSE 29
43. 36 EEC 93 42 EEC This product conforms to the following Harmonized International and National Stendards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 MDD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 EE Richard L Miller COE Regulatory Compliance Manager Z o zz Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Meclarai na nf Contormito Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Atfected Units Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 U S A IVF Controlled Rate Freezer 7476 2001 Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC MDD 89 336 EEC 93 42 EEC This product conforms to the following Harmonized International and National Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 MOD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 DATE db Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 AA 4 7007452 CryoMed Freezer ThermoFisher SCIENTIFIC 18 June 2008 Thermo Scientific Thermo Scientific Handling Liquid Nitrogen Introduction Warning Contact of liquid nitrogen or cold gas with the skin or eyes may cause serious freezing frostbit
44. 5 minutes The probes must be in the center of the container well away from the container walls Observe the Chamber and Sample temperature displays Adjust the Chamber and Sample Scaler parameters so that the displays each read 196 C If several scaler values result in the desired reading select a scaler value in the middle of the range Repeat Steps 3 through 6 as necessary until both temperature displays read 0 0 C and 196 C There may be some interaction between the Offset and the Scaler adjustments Thermo Scientific Thermo Scientific Menu Entry Definitions cont Section 3 Configuration Calibration 3 Service This menu is for use by qualified service personnel Entry into this menu has a required service access code Warning This area may be accessed by qualified service personnel only A A Manual Control Individually or in any combination turns on fan heater Cool solenoid and Cool solenoid for diagnostic or troubleshooting purposes and displays the status of each component Upon entering the menu the Enter and Back keys position a cursor over the item to control The up arrow turns On the selected item and the down arrow turns it Off When the menu is exited Back key any function that has been turned On will be turned Off and the unit reverts to Idle Mode Note Fan will run whenever the heater is energized The solenoid closest to the front of the unit will be energized when Cool i
45. 50081 1 92 EN 50082 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 _ Z Z z Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Thermo Scientific AHeeluritim of Contormity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description IVF Controlled Rate Freezer Product Designations 7459 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC MDD 93 42 EEC This product conforms to the following Harmonized International and National Standards EMC MDD EN 61328 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 Richard L Miller COE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 15 November 2007 Declaration nf Conformity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Milicreek Road Marietta Ohio 45750 U S A Product Description Controlled Rate Freezer Product Designations 7473 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC LVD 73 23 EEC This prod
46. EE EISE 40 8 Z 94 1006 OSI uoneuuoJur AJU amp JJeM JO Jo1nquisip e90 Jno D IUO YSN au episino uoneorjdde eroeds pue sonas eoueuejureui uonejado AueJe juaudinba uo suonsenb noA Jamsue o Apea 9J 9M 9 t5 7 0v4 L Jo epeue pue VSN 06 1 1Z 888 1 1e jueuniedeq sanas eoiuuoe Ino jeo aseajd peJinbeJ s aomies jueuudinbo y SjonpoJd JO SSO JO s iJoid ISO 0 Bunga sabewep uoneyu1 joyym Buipniou sebewep jenuenbesuoo Jo Daul Aue 104 a qe1 aq zou jeus oway 82UEUO UIEUJ SAI u A 1d pue uonejedo uonej ejsur jueuudinbe rejep AjjnJoseo sjenueuu uononiulsui paud 064 1 1Z7 888 1 eoejajul Od pue J9Z99 4 9 8y pojo41uo2 ay uo suonsenb euoneJjedo pue dn jes JO pojoejuoo aq Aew juauuedag seoliues eoluyos oul pepeeu se WOU j9e uos esee d J9JnjoejnueuJ jueuoduiJoo y 0 paulajal eq jsnuJ sjueuoduuoo JejnduJoo y JO Aue uo uenem aul ALNVAYVM V 3Hdl l3d GNV d OIdILN3I9S H3HSI3 OIWNSHSHL 11 1 7007452 CryoMed Freezer Thermo Scientific LUIS c 1929 ELENODEL 1006 OSI uoneu0Jul JUeJem JO Jojnqujsip e90 Jno joejuos ysn 24 episjino uoneoirdde eio eds pue a91nas eoueuejureui uonejado AjueJem jueuudinbe uo suonsenb jno Jamsue 0 peaJ 949M 9 t 0tv4 L Jo epeueo JO veni OBZL ELZ 8g8g l 1e jueuniedeq saseg oway JNOA peo eseejd peuinbaJ s asnos jueuudinbe eoueuojuleuJ AlJUu A 1d pue uonejedo uonejeijsur jueuudinbe ie1 p A
47. IS POW AUueJIeM Su L ALNVSHSHVM 4323343 31V8 GATIONLNOO OIJILN3I2S H3HSIJ4 ONSHSHL 11 3 7007452 CryoMed Freezer Thermo Scientific bERZT e 10 Z Z Au 1006 OSI UoneuJJoJur JUe Jem JO JOJNQIISIP 890 Jno DEIUO ven 24 episjino uoneordde eio eds pue sones aeoueuajulew uonelado AUS UE juaudinba uo suonsenb noA JaMSUB o Apea 24 2M 9 t 0p L Jo epeueo JO YSN 064 1 1Z7 888 1 1e juauyuedag samas jedIUYDE Jno jes eseejd p nb s aces u uudinb y eoueuejureuJ AlJU A 1d pue uoneJedo uonejejsur 3ueuudinboe reyep Anae sjenueu uononJsut pejulud SeALJe jueuudinbe Ino aJojeq uoneuuojJur uonejedeJd ous eArisueueJdujoo YM dieu oj pea si au sejes ouueu e20 INOA SjonpoJd JO sso Jo SHJOIA soo DunejoJ sobewep uonejyuui joyym Buipnjour seBewep jenuenbesuoo Jo yapu Aue 104 a1qe1 eq jou UEUS ELO oway XlddV TIVHS ISOdY4NdA YVINDILYVd V 902 SSANLIS YO ALI TISVLNVHOSIN dO SILLNVIYVM ON CSI Idi MO IVYO N3lLISM Y3HLIHM SSILNVYYVM YSHLO TTV dO NAN NI ANY 3AISNTIX3 SI ALNVYYVM SIHL uoneunsep GO 4 peddius se sued jueujeoe daJ pue pied eBejsod OUJJ9U 0 Dome aq jsnuJ sued eAnoojep e uondo s ouu u yy 1ueuudinbe Jo sjueuodujJoo Aug jo Una 104 enoJdde Joud auf ysnw juauuedag s oIAJ8S eoiuuo9 au poned uenem eurBuo eu puo eq yed 3jueuoduioo y o JO jueuudinbe y Jayya o jueJem sy puojxe JOU eys AJUeWeEM siy Jepun 1u uudinb Jo sped sjueuoduioo Jo Jeda JO juauaoe d
48. LS CONTROL HOUSING ASSEMBLY 7452 201 3 B REV PAGE 1 OF 2 Thermo Scientific 7007452 CryoMed Freezer 7 9 Section 7 Parts List 7 10 7007452 CryoMed Freezer BILL BF MATERIALS ITEM PART NU PART DESCRIPTION DIY l 22052 48 32 X 3 8 SS PHP SCREW 16 2 23002 48 32 ZP LKWASH HEX NUT 8 3 23010 8 32 SS HEX NUT 2 4 23021 8 SS FLAT WASHER 4 7 30004 3 8 X 1 4 ID GRUMMET 6 35021 378 ID X 378 WALL ARMAFLEX TUBE 7 73135 3 4 SS INT TOOTH LOCK WASHER 8 107406 1 4 X 7 8 WHITE PORON TAPE GASKET 9 117018 7 16 DIA BLACK HOLE PLUG 2 123020 NYLON EDGE PROTECTOR l 180216 PRINTER WINDOW PAPER CUTTER 2 180230 BULKHEAD HEATBREA 3 201183 THERMAL DATA PRINTER 4 201185 POWER ADAPTER 120VAC BLUZ 7 201187 230V SOHZ PRINTER POWER SUPPLY D 251008 1 4 MPT 60 PSI RELIEF VALVE 7 380181 1 4 X 80 BRASS SIREET ELBUW B 380260 1 2 FLARE X 1 4 MPT CONNECTOR 9 400148 200F MANUAL RESET T STAT 20 440022 PUSH MOUNT TIE AND ANCHOR 2l 460024 SNAP IN OUTLET WHITE 22 460128 220V EUROPEAN RECEPT W COVER 23 590020 48 32 X 3 8 SS PHP EXT SEMS SCREW 10 24 590027 6 32 X 1 4
49. Last step in profile Maintains the last Chamber temperature from the previous step indefinitely until the user terminates the Run with the Stop button or from the freezer keypad Follow the procedure below to create a new freezing profile jm Select the desired first step type from the Step pull down menu WAIT or WAIT FOR TEMPERATURE is the mandatory first step It is required to purge the warm LN2 plumbing prior to initiating controlled rate freezing This step cannot be deleted or changed to a different step type Select subsequent steps Use Insert to add a step The new step is added on the line that is highlighted Use Replace to modify an existing step s parameters or change to a different step type END is the mandatory last step It alerts the freezer that the profile steps are completed This step is automatically added during the profile creation It cannot be deleted or replaced 7007452 CryoMed Freezer 10 7 Section 10 PC Interface Software 10 8 Create a New Freezer Profile continued Edit an Existing Profile Print a Profile Listing 7007452 CryoMed Freezer Save the profile by selecting the Save button Enter a profile name and Save A comment may be saved with the profile Comments are printed with the Run data Saved profiles are given the extension prg Select the Delete All button to clear the program listing window There can be a maximum of 20 steps including the End step A saved or al
50. List BILL Dr MATERIALS ITEM PART NU PART DESCRIPTION QTY l 20017 1 4 20 X 3 4 ZP HH CAP SCREW 2 2 22015 58 32 X 3 4 SS PHP SCREW 4 3 22052 58 32 X 3 8 SS PHP SCREW 7 4 23001 86 32 ZP LKWASH HEX NUT 2 9 23010 8 32 SS HEX NUT l4 6 23021 8 SS FLAT WASHE 14 7 23080 8 SS SPRING LDCKWASHER 18 8 58000 174 20 X 1 2 ZP PHP SCREW 4 9 103112 REMOVABLE BULB GASKET l 0 115027 5 16 DIA S S HANDLE l l 180226 1 2CF CRF CABINET HEAT BREAK FRAME l 2 180231 CONTROLLED RATE FREEZER EXHAUST VENT l 3 190612 ALUMINUM HINGE BLOCK l 4 360235 SURFACE MOUNT MAGNETIC SWITCH l 9 505180 2 DIA BLACK RUBBER FDDT 4 6 580020 58 32 X 3 8 SS PHP EXT SEMS SCREW 2 7 610073 3 8 18 NPT NYLUN JAM NUT 2 8 860032 3 8 MPT CPVC X 3 LUNG NIPPLE 9 880000 INTERIUR CHAMBER SUB ASSEMBLY 20 890001 1 2CF EXTERIOR CABINET l 21 890003 INTERIUR CHAMBER SUPPORT 3 22 890004 _ UPPEK RIGHT CHAMBER SUPPORT 23 890010 CABINET HEAT BREAK END RETAINER 24 880018 RETURN AIR BLOCK 3 25 890021 DOOR SWITCH MOUNTING PLATE SUB ASSEMBLY l 25 890023 TDUP AND BOTTOM HEAT BREAK RETAINER 2 27 880030 SIDE HEAT BREAK RETAINER 28 880052 HINGE SH 2 29 890053 SHIPPING BRACKET 30 900179 ALUMINUM HINGE BL
51. Model 7452 Series CryoMed Controlled Rate Freezer Operating and Maintenance Manual 7007452 Rev 11 S Preface Non IVF Units IVF Units Software Addendum Model Voltage Model Voltage 1 Installation of the National Instruments LabView Run Time Engine 7450 120 7456 120 is required for proper operation of the PC Interface Software 7451 230 7457 230 2 A PDF version of the Windows Help file for the PC Interface 7452 120 NES d Sola is located on Ge CD ROM The file name is Help PC Interface pdf 7453 230 7459 230 3 A Read Me file containing the known issues with Version 1 0 of the 7494 120 7419 100 PC Interface Software is located on the CD ROM The file name is 7455 230 7476 100 ReadMe txt 7472 100 4 The Windows Help file can be accessed in the PC Interface Software 2473 100 Edit Run Review or Options screens by depressing the F1 key on the PC keyboard 7474 100 5 If password protection is enabled note that user names and passwords are case sensitive MANUAL NUMBER 7007452 11 25334 2 24 09 Removed LN2 Supply pg 2 14 CCS 10 24349 FR 1980 3 26 08 New power supply with power cord 1950135 CCS 9 24391 1 21 08 Added PC operating system specs to Section 10 CCS 8 24326 S1 9956 12 19 07 Removed all 10 00 kits obsolete CCS 7 23738 FR 1928 10 03 06 Corrected the calibration procedure for a recorder Pg 3 1 CCS 6 22854 FR 1860 5 11 05 Removed registration she
52. NIAE CU we dep as ARENA at Ye te d wa ad Nod dye DOS d res 1 3 Output voee Ea TET prc Wes A AS H RAN T ede 1 3 EE 1 4 Set Up and Operation 2 1 Supplies io eee euo tenes A eee dH ee eed 2 3 Electrical Connections oed oso seite Ese d set aet tet Ee 2 3 Environmental Conditions 2 3 Sample Thermocouple Connection tt eee oe Kei ase 2 4 Power U us aaa yqa deba aides aaa d ua 2 4 Access Code ett ee ed RO LUE E BAE E E E SAU 2 5 Initiate a Profiles a ett dE EE e dE E ond es 2 5 Stop a Profile Ruan ti Pe ER PS EROR dus 2 6 Manual Override qayaspa esi E L Gu eec Le 2 7 Program User Specific Profiles AB o as cp D UO uer 2 7 Copy and Paste Pre set and User Profiles 2 10 IT ulises Se Lon aret Ea da hioa 2 11 Printet citar A deua n tt 2 11 EE 2 12 Chamber Thermocouple Open 54 ac ess eher prive ue T Yu 2 12 Sample Thermocouple Open 2 13 Heater Malfunction caos suy h HM urn e Lc Ua des 2 14 Chamber Sample Over Under Limit Temp 2 15 Power EE dard aep acp i epa 2 17 Run Complete core Bee grd pte Eet See keke ids 2 17 Warm Completa Costco OE Su Su R TR R M EAE f 2 18 Seeding Hold aca edes PPS d NES brasa 2 18 Printer Buffer Eull roo desk Re ars ed iis 2 18 IVE Sample Holdes ul tpe eee eq o tees 2 18 Using a Vial Holder utentes a da be cm e Se RR dues 2 19 Using the Straw e Abee eal A bebe aia 2 19 Using the Cane Ho
53. OCK 30 880032 0 86CF INTERIDR CHAMBER SUB ASSEMBL Y 3l 880033 10 DE EXTERIOR CABINET l 32 890037 0 BCF RETURN AIR BLOCK 2 33 880038 10 BLE TOP AND BOTTOM HEAT BREAK RETAINER 2 34 180225 O 6CF CRF CABINET HEAT BREAK FRAME l 35 880041 1 7CF INTERIOR CHAMBER SUB ASSEMBL Y l 36 890042 1 7CF EXTERIOR CABINET l 37 890046 1 7CF RETURN AIR BLOCK 2 38 890048 1 7CF TOP AND BOTTOM HEAT BREAK RETAINER 2 39 180227 1 7CF CRF CABINET HEAT BREAK FRAME l CABINET ASSEMBLY 7452 201 1 B REV 3 PAGE 2 2 7 6 7007452 CryoMed Freezer Thermo Scientific Section 7 Parts List PART DF ITEM 22 CONNECTS INTERIOR CHAMBER 7 TO EXTERIOR CABINET a MOUNTS TO RIGHT WALL OF INTERIOR CHAMBER ABOVE COIL FUR T STAT BULB RIGHT END VIEW n E CABINET ASSEMBLY 7452 201 2 B REV 2 PAGE 1 2 Thermo Scientific 7007452 CryoMed Freezer 1 7 Section 7 Parts List BILL OF MATERIALS ITEM PART ND PART DESCRIPTIUN QTY l 20004 1 4 20 X 1 SS HH CAP SCREW 4 2 22002 4 40 X 1 2 SS PHP SCREW l 3 22052 8 32 X 3 8 SS PHP SCREW 8 4 23002 8 32 ZP LKWASH HEX NUT 4 5 23005
54. PLY SWITCHER L AR MN 230185 104 MOTO 370524 B PUS PLLOLARLE AE 7 REFER TD DETAIL 3 FIR WIRING LZ A 1830017 _ EN CONTROL BOARD MINI i lt 202 M LOCKWASHER P 205 8 32 X YR SX f E zm seid ye 2358 8 LOCKWAS ER N HET MAT e ckt T A 195718 WAIN HARNESS i S 440022 PUSH IN TIE WAN dimi d SECENDAY CONNECTION 2 PEN FETAL 3 TERMINAL BLOCK WIRING DETAIL f m Ee 1890053 SHIPPING ait d A I JU dall ffo oooooc D b aD H AA 20017 1 4 20 X 3 4 Eed L le 00y TIE WRAPS Ji lt H AN al 4 REQ WITH IN 1 2 OF TERMINAL BLOCK Wiring Diagran On Model SOS ERR 0 miel es x s ce WHITE WIRE FROM THERMOSTAT 7451 230N inm Controlled Rate reezer BLACK WIRE RO SALLE WIRE ERIN NOTER _ N S Ve E Gi DEI NOLO WIRES ERI JUS S ID 33 gt E ARE RES FROM S PSI YW V 145 71 1 D REV 2 Page of 2 7007452 CryoMed Freezer 7 17 Thermo Scientific Section 7 Parts List S WIRING OETATL PRINTER AN Berdorf S EN W 15 DETAIL 4 THERMOSTAT WIRING DETAIL DETAIL 3 PRINTER CUTLET E wea 9 U inis I a WW ROIN 30037 TIE RIP 30001 TIE ANCHOR V 8 2 KEPS NIT 4 RED WIRES TO RED AND BLACK U REAR AND HP VI WIRES 6 KEPS NUT 300132 FAN 432011 FAN GRILL 22010 6 32 X 1 1 2 SCREW 4 REN PAR VEL RV EL 22043 6 3 X 3 8 LR 2 REN Pres e e cx 2 SB SCREW LICK SE RE w
55. R 230 V COOL VALVE 250084 COOL PLUS VALV 250084 Electrical Schenotic Mode 7451 7453 7455 and 7457 Control Rate Freezer 7451 70 1 0 REV 2 Page 1 of 2 7007452 CryoMed Freezer 8 3 M D iy LO CO 24 24 22 x EI ED Es Ludo m3 mx gl Ee eg CH P A EP Ss Lu P CI CIC dl Ul co Cn A 1 O J C B D G YE CH sd 110 co co 1 ZO E E H NE 8 2 18 RED 59 22 18 CRN YEL 50 6 EXTERNAL CONNECT TONS C CONN SUBM PIN 2 TRANS ECEIV PIN 5 SIGNA CONNECTOR ANALUG RECORDER DUPLT O 10VOC NONLINEAR LE SUBMINTATURE D CONNECTOR 2 PLUS 4 MI ec T S o 2 FR 1582 03 08 02 RTT PDK AKS ADD IVF PROBE CONNECTIONS E ctr Ico Schema IC 1 SL B200 6 18 01 RTI RTT BH CHANGED WATTAGE FROM 1000 Model 0 N A 5 29 01 RTT STT BH RELEASED FOR PRODUCTION 5 EUN E REV ECN NC D BY LU leen DESCRIPTION UF REVISION 7451 j 1453 i455 THIS DOCUMENT CONTAINS PROPRIETARY ART NAME A EZER 7451 53 55 57 amp 59 TE INFORMATION AND SUCH INFORMATION IS NOT TO Ec TET NAME CUNT RATE FREEZER 7451 53 55 57 amp 59 and 7457 AN ATTENTION HE DISCLUSED TD DIHERS FUR ANY PURPUSE NUR DWG TITLE ELECTRICAL SCHEMATIC E tn POSES WIT to E Y A OBSERVE PRECAUTIONS L FERME ENT SEM EMIT OWN RTT CAD RTT APPD BH DATE O1 0
56. SS PHP EXT SEMS SCREW 8 25 640037 DUAL LOCK FASTENER TYPE 400 2 26 640038 DUAL LOCK FASTENER TYPE 170 2 27 790008 3 8 COMP X 1 4 MPT BRASS CONNECTOR 4 28 800031 1 4 FPT ANCHOR COUPLING 29 800032 1 4 PIPE MALE BRANCH TEE 30 890014 PRINTER CLAMP 2 3l 880015 PRINTER COVER SUB ASSEMBLY 32 890022 VALVE BUX BRACKET 33 890023 DRIP PAN SUB ASSEMBLY 34 89002 COMPUNENT MOUNTING BRACKET SUB ASSEMBLY 2 35 890026 EXPORT POWER SUPPLY HDLD DDW 36 890031 PLUMBING INSULATION SUB ASSEMBL Y 37 890049 LN2 INLET PLUMBING SUB ASSEMBL Y 38 890050 LN2 OUTLET PLUMBING SUB ASSEMBL Y 38 890051 PAINTED CONTROL PANEL BEZEL 40 890054 BULKHEAD RETAINING PLATE 4 850135 BRACKET FDR 201185 PRINTER PDWER SUPPLY 2 000617 120V BRASS SOLENOID VALVE 2 43 CONTROL HOUSING ASSEMBLY 44 201186 100V 50 60HZ PRINTER POWER SUPPLY JAPAN ONLY 3 000618 220V BRASS SDLENDID VALVE EXPORT ONLY 2 CONTROL HOUSING ASSEMBLY 7452 201 3 8 REV PAGE 2 OF 2 Thermo Scientific Section 7 Parts List MBL Y PIN ASS 0 250 HING SCAL ASSEMBLY l EV R CABINEI 7452 201 4 PAGE 7 11 7007452 CryoMed Freezer Thermo Scientific Section 7 Parts List
57. Suiddeus 1030u1 3291107 ez uio qoud Apddns jy 190110 z 10jour ooe dow pI 3uiddeys OVAOEC OCI 1991107 91 101001 ooe dow 01 uon onnsqo aruru o jsn pe aAO A eq esnyooe dow 1 JOU JI UO IOJOW A opour jenueur UI 10100 S590108 OVA 02 06 DEU o1our amseo K ddns jy SA 1ojour ut Surddens jyvAOEC OCI ez UO UJ tA Opo enueui ur 1070W SOJ98 DVALOT 06 ue sso Jt MOO c manos Sutddens DY AOECC OCI pu juo1mo 10100 oAIssooXo 101 YOMO PT JOU JI 10910901 Suiddens jvAOtCC OCI ed OUIVU SA JUANI 10 OUI DAISSIIXI 101 oou OT YeYS JOJOW JO UONRIOI 99 101 JOMO qq UR Jo uorejo1 pojonnsqoun 101 JO eq en eA osng yooyD I preoq 19ITOH00 SuneauoAo 1030U1 JOJOU ur jno jno JeW c pieoq 1o o nuoo uo asny 1 uni JOU IM UB Y p1eoq 1o o guoo ooe dow e sprouo os ooe dow y Suiquin d esoq opeisdnyooe dow loje ngoj xuej ooe dow z yue qg eoepdosp T oyenbope st jddns OV pue prouo os ssoJoe IV ALOZ 06 uet Ss JI s dom ounje1oduro 10 punos ou pue sprouo os ssoJoe V A 0c 06 UY o1our JI dom ounje1eduro 1equieqo pue opour enueur ut LWO se3 29 punos uorezado prouo os Joo p 3urquin d poje nsur ummnotA JO yue 0j OUT XY 9 p Teururou 77 10ye n391 ounssoud yur AJA C oyenbope sjuojuoo yur A ddns AUSA T peq preoq s llonuoO peq sprouajos NT y Suo oo asoy jddns
58. TE If the temperature is already set to 0 C move it up or down 10 C then back to 0 C The recorder pen should move to 0 C If it does not adjust it using the zero knob on the recorder until the pen reaches 0 C Set the temperature to 180 C to simulate the low end of the scale The recorder pen should move to 180 C If it is out of calibration adjust it using the small screw accessible through the hole adjacent to the zero knob until the pen reaches 180 C Recheck zero calibration as directed in Step 4 Repeat Steps 4 and 5 if necessary C Printer Menu Selects the thermal printer operating mode printer scale or initiates printing of the previous run chart Select the desired item using the Enter and Back keys and change the selected parameter using the 5 6 keys Exit the menu using the Back key Thermo Scientific Thermo Scientific Menu Entry Definitions cont Section 3 Configuration Calibration a Select Printer Output Select Chamber Only Sample Only Chamber Sample Scan or No Printer Off The default is Scan b Enable Disable Profile Set Temperature Line Turn On or Off a line on the thermal print out that shows the set temperatures and ramps as the freezing profile is run and printed The default is Off c Previous Run A duplicate of the print out made during the Run The print out reflects the actual time and date of the run rather than the current time and date Note The unit wi
59. Tag This product conforms to the following European Union Directive s EMC LVD 89 336 EEC 73 23 EEC This product conforms to the following Harmonized Internationa and National Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 UL 61010A 1 DOLO Z DIL Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Beclaration of Cantoria Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Units Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 USA Controlled Rate Freezer 7452 2001 Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC LVD 89 336 EEC 73 23 EEC This product conforms to the following Harmonized International and National Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 UL 61010A 1 EN Richard L Miller CQE lt Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Thermo Scientific Declarat na of Confarmitn Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description C
60. WITCH WIRES TO LUNN SWLICAH WIRE i Ea L 23058 4 STAR LE E Je HE SWITCH CR MAGNET USE GL Up I mul d EZ z ou TAS 2 PUS CONECTOR ANO LN REFER TO DETAIL FOR WIRING WILL 370351 STRAIN RELIEF 5 2 Y A SEI 01 EN Ce oen mm e 4 X 2010 DISPLAY FCRI wn I SERVICE WG UE IT YUWE POI J USE Q 4000619 Le C vm E AlL 2 V EL ARA WIRING DEAL de 330001 50003 2 8 SPLIT SNAP BUSHING 2 56 X 1 4 SR 4 RED end ERO WIRE LCD DISPLAY CABLE PODER SOLDER CABLE TD LCD WV CONNECTOR POSITIONED AS SHOWN i PRINTER CONNECTOR PART OF 195712 85 232 CONNECTOR PART F 195712 I H C 9 m 390020 8 32 X 270 SEM SCREW l N I X X 38H N BLUE MUTE WIRE a 23 TE GREEN YEL WIRE DC FAN CONNE 195722 MAIN HUT DC PONER 59007 4 40 X 3 8 SCRE 23022 4 LOCKWASHER JN DUAL INPUT SAMPLE PROB CONNECTOR IVF MODELS BNL E g 370249 SAMPLE THERMICOLPLE PLUG Wi ji i Y m j 123020 EDGE PROTECTOR 6 LONC SUPPLY INSTALL 12 DF 3 4 SHRINK 246045 ON VALVE WIRE SET SHRINK IN PLACE AND INSTALL A SMALL N TIE WRAP 30037 1 FROM EACH END INS CHANGER THERMIECIPLE UU 191648 M 20037 TIE WRAP a r HI p 30001 TIE WS 00132 23002 KEPS NUT NOMER SUP
61. Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv 7007452 CryoMed Freezer Thermo Scientific Thermo Scientific Section 1 Section 2 Table of Contents I tr d ctioi uice c enevk PIN RIS Ri waa ERI DIEA TRIS RES 1 1 Standard Features aa K duce dt it EN 1 2 Door latch it A tenuto pip ae a Sk Ead 1 2 Sample Seeding Access Ports IVF 1 2 A TT 1 2 indicators ia EE 1 3 Keys estas bie ub peni eade tub ebbe tud 1 3
62. able ports are shown on a 10 pull down menu If a port is in use by another freezer the port will not appear on the menu Click Continue or Cancel to abort start Select a freezing profile The File Select window opens at the default directory chosen in the Options screen Choose a file or change directories then choose a file Click Open to continue or Cancel to abort the start of the Run As the Controlled Rate Freezer goes into Remote Mode and profile is downloaded and validated then the freezer keypad is disabled except for the Silence key Enter the mandatory Data Tag Select a word or abbreviation to identify the Run The Data Tag is used as Run data file name prefix followed by a sequential number generated by the application The Data Tag information must be entered before the Run can be started Note If illegal characters U V lt gt are used in the data tag the program runs normally but no data file is created Enter any optional data in previously enabled Information Fields Up to 12 fields can be enabled and named in the Options screen The information entered is captured in the Run file and displayed on the Review screen and information page print outs Select the starting step of the profile Default is the first step in the profile Any step can be chosen to begin the profile Initiate the profile Run by clicking the Start button Enter optional comments The text entry
63. ager ThermoFisher SCIENTIFIC 18 June 2008 Thermo Scientific Declaration nt Contormita Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Units Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 U S A IVF Controlled Rate Freezer 7458 2001 Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC MDD 89 336 EEC 93 42 EEC This product conforms to the following Harmonized International and National Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 MDD EN 61010 1 1993 Amendments 1 and 2 CSA C22 2 No 1010 1 UL 61010A 1 Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Mectorntion nf Contormity Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Units Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 U S A Controlled Rate Freezer 7472 200 Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC LVD 89 336 EEC 73 23 EEC This product conforms to the following Harmonized International and National Standards EMC EN 61326 1 1997 EN
64. an audible tone See Alarms Section 2 Graphs and cycle data as well as additional information are printed out at the top of the unit on a thermal printer in real time mode A connector is also available on the back of the unit for an optional strip chart temperature recorder 7007452 CryoMed Freezer 1 3 Section 1 Introduction Pre set Profiles The Controlled Rate Freezer has six pre set freezing profiles They are labeled Pre Set Profile 1 through 6 These profiles cannot be edited To view the profile timing and temperature 1 Scroll using the up or down arrow keys to the desired profile 2 Press the Enter key to go into View Mode Scroll using the up or down keys through steps as needed 3 Press the Back key to return to the main menu The pre set profiles are listed below In addition a Configure Calibrate menu is available For further information on this menu see Section 3 User specific profiles can also be entered and saved See Section 2 Pre Set Profile 1 Commonly used for 2 0ml sample size resulting in a 1 C rate from nucleation to 40 C and a 10 C per minute cooling rate to a 90 C end temperature Sich Lecce abe ater Rd e rr S bea wees ette Wait at 4 0C KI out ene Antia doo dO s P wet 1 0 C m S to 4 0C BICI RAT ot bona a opa ts 25 0 C m C to 40C WO NOTER e 10 0 C m C to 12 0C Step D centu In EO REPE bea es 1 0 C m C to 40C SEP curae ete detur eruat dinde 10 0 C m C to 90C EE End Pre Set Prof
65. ay AE UE SIU wo pepnjoxe aJe sjejuud eut 9 SJOWUOW sjeinduuoo euosiad sjeyxseb say sseJ 6 sway ejqepuedx3 Spa Aue jo eoueuuoged oj soud uonoeJuip pue uoneuruJojop jueJem JO poejoejuoo eq 3snu juaeunedag Soul jediuyoe eu 3ueuie9J6e jueJem siy Ag pajaros JOU Se uoneuaqijeo pue uonejejsu papn 9xa Joqe esuedxe s ouuau ye peoe dau Jo paJredaiu eq Iw diysueuyJom JO sjenajeu ui Buruuojuoo uou aq oj UBAOJd sued jueuoduioo jee 38111 ayy Buunq jueunjedeq seoiues JEDIUYD9 sy 0 peuunjeJ pue poeje duJoo si DIE jueJem sy pepri oJd uonejjejsur pue lanIjap 104 SYJUOW xis euonippe ue pamo e ase juaudinba Jno 390 s oym SJe eeq poled Ajue amp uew Jes 1sJiy eu Buunp saumo juenbesqns Aue o spuejxe uon 998 01d JUeJem SUL peJeAr ep SI jueuudinbe JNOA aun wes y jejeuwixoldde je joaya our oB jm uenem ay os aun Buiddiys 104 smoyje et Aye ino wouy peddius si juaudinba noA app ay wo suluouu OM SUEIS pode AIUEUEM Su L ALNVJAYVM 43 1V3G 43Z233Y3 299 Q3TIOHINOO TIWNOILVNYSALNI OIdILN3I9S Y43HSIA OWYSHL Thermo Scientific 7007452 CryoMed Freezer 11 4 Declaration nf Contormity Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Units Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 U S A Controlled Rate Freezer 7450 2001 Release 2 Release Level REL shown on Serial
66. common sense procedures based on that knowledge There are two important properties of liquid nitrogen that present potential hazards 1 ltis extremely cold At atmospheric pressure liquid nitrogen boils at 320 F 196 C 2 Very small amounts of liquid vaporize into large amounts of gas One liter of liquid nitrogen becomes 24 6 cu ft 0 7 m3 of gas The safety precautions in this manual must be followed to avoid potential injury or damage which could result from these two characteristics Do not attempt to handle liquid nitrogen until you read and fully understand the potential hazards their consequences and the related safety precautions Keep this information handy for ready reference and review Note Because argon is an inert gas whose physical properties are very similar to those of nitrogen the precautions and safe practices for handling and use of liquid argon are the same as those for liquid nitrogen A 7007452 CryoMed Freezer AA 5 AA 6 7007452 CryoMed Freezer First Aid Use containers designed for low temperature liquids Cryogenic containers are specifically designed and made of materials that can withstand the rapid changes and extreme temperature differences encountered in working with liquid nitrogen Even these special containers should be filled SLOWLY to minimize the internal stresses that occur when any material is cooled Excessive internal stresses can damage the container Do not cover or plug
67. d in the print out selection window Vertical scale may be automatically selected for best presentation 1 Compare file Profile name and path 2 Compare Run data file and path 3 User name 4 Date time 5 User Information Field name contents 6 Run start stop comments 7 Profile listing 8 Alarm listing with time of occurrence 7007452 CryoMed Freezer 10 21 Section 10 PC Interface Software User Controls continued 10 22 7007452 CryoMed Freezer Exit Button This button may be used to exit the Review screen It is not necessary to close any files before exiting Export Button This button may be used to export the open Run profile data into a comma separated variable format The data can then be imported into standard spreadsheet or database programs The data includes the data tag date time user name information field name and contents source data file name profile steps elapsed time profile temp Chamber Temp Sample temp run data and any alarm with occurance time Scale Select Pull down Menus Temperature division Click down arrow to select 20 10 5 2 19C per division Time division Click down arrow to select 20 10 5 2 1 5 1 minutes per division Graph cursor and Chamber Sample temperature windows used to indicate the Chamber Sample temperature at a selected point on the graph The default cursor position is the left side of the on screen graph yellow vertical line Point at cu
68. e Remote Run the control panel keypad is disabled with the exception of the Silence key The PC software communicates with the CRF through the RS 232 serial connection included with the freezer is a 10 male female DB9 cable and a DB25F to DB9M adapter Data information is routed with a check byte for transmission integrity The software stays in continual touch with the freezer to detect any communication breakdown A single PC can communicate with up to four Controlled Rate Freezers using separate Run windows To connect a PC to more than one CRE install a multiple port serial interface card in the PC Interface cards should meet the following specifications 9 pin RS232 ports 1 communication port for each freezer Support baud rates up to 9600K baud minimum 16550 UARIS suggested 128 byte transmit and receive buffers FIFOs suggested Automatic assignment of ports suggested 7007452 CryoMed Freezer 10 1 Section 10 PC Interface Software Installation amp Set Up Make sure the cable between the Controlled Rate Freezer and the PC is connected properly To install and set up the PC software locate and execute the Set Up exe file on the software CD by selecting Start then Run The software is installed in the default directory Additional directories are created for the freezer profiles Programs and run data Run Files The PC software must be registered If after 30 days the software has not been
69. e injury A Handle liquid nitrogen carefully The extremely low temperature can freeze human flesh very rapidly When spilled on a surface the liquid tends to cover it completely and intimately cooling a large area The gas issuing from the liquid is also extremely cold Delicate tissue such as that of the eyes can be damaged by an exposure to the cold gas which would be too brief to affect the skin of the hands or face Never allow any unprotected part of your body to touch objects cooled by liquid nitrogen Such objects may stick fast to the skin and tear the flesh when you attempt to free yourself Use tongs to withdraw objects immersed in the liquid and handle the object carefully Wear protective clothing Protect your eyes with a face shield or safety goggles safety glasses without side shields do not give adequate protection Always wear gloves when handling anything that is or may have been in immediate contact with liquid nitrogen Insulated gloves are rec ommended but heavy leather gloves may also be used The gloves should fit loosely so that they can be thrown off quickly if liquid should splash into them When handling liquid in open containers it is advisable to wear high top shoes Trousers which should be cuffless if possible should be worn outside the shoes The safe handling and use of liquid nitrogen in cryogenic refrigerators and dewar flasks is largely a matter of knowing the potential hazards and using
70. e 1 2 IVF Controlled Rate Freezer FDA clearance for human research 7007452 CryoMed Freezer 1 1 Section 1 Introduction Standard Features Door Latch Sample Seeding Access Ports IVF Displays 7007452 CryoMed Freezer Active solenoids Alarm indicator CT teat KR Active relay Operating mode indicators Alarm silence key Chamber BK Sample Temperature Display LCD Up roi oom Back Enter 4 D Warm pon Figure 2 3 Control Panel Displays Indicators and Keys The door of the Controlled Rate Freezer is secured in the closed position with a vise action T handle latch The latch maintains positive compression of the door gasket against the Teflon heatbreak to prevent leakage and ice build up during normal operation The latch is closed by turning the handle 180 degrees clockwise Note Make sure the door is properly latched during operation to prevent ice build up A The IVF model of the Controlled Rate Freezer has top mounted sample access ports used to seed the samples during the freezing process Seeding should always be performed through the access ports rather than the door to reduce sample temperature fluctuations during the seeding process There are three displays on the Controlled Rate Freezer control panel Chamber and Sample temperature ranging from 454 to 199 C the LCD Disp
71. ecified Sample temp the Sample is cooling Note The sample temp must be greater than the chamber temp A 10 4 7007452 CryoMed Freezer Thermo Scientific Section 10 PC Interface Software Edit Screen FEET continued Command Ramp e Rate 3 Ome Repl Temperature ee Zu J 3 Sensor Chamber Delet Figure 10 4 Ramp Rate Step Name with Menu Format Step Parameters with Definitions Ramp RAMP X X C MIN UNTIL CHAMBER Y Y C or RAMP X X C MIN UNTIL SAMPLE Y Y C where X X is the ramp rate and Y Y is the chamber or sample endpoint temperature Changes Chamber temp from its current value to specified temp Chamber sensor trip point at the selected rate C minute The rate can be set over a range of 0 1 C minute to 99 9 C minute The temp can be set over a range of 180 C to 50 C 0 1 C When the Chamber reaches specified temp the control moves to the next step in the profile If Sample sensor is selected Chamber temp changes at a specified rate until Sample temp reaches specified temperature Note The slope of the temperature ramp is determined automatically based on the temperature entered and the chamber temperature at the beginning of the step Entry of a sign for the rate term is not required allowed A Thermo Scientific 7007452 CryoMed Freezer 10 5 Section 10 PC Interface Software Edit Screen continued 10 6 7007452 CryoMed Freezer Command Hold M Chamber 40 HE
72. ed over a range of 0 5000 to 1 9995 in 0 0005 increments Adjust the probe scaler for a 196 C temperature display reading with the probe in liquid nitrogen Warning Contact of liquid nitrogen with the skin or eyes may cause serious freezing frostbite injury A Thermocouple Probe Calibration Procedure Note A container of pooled liquid nitrogen and a container of crushed ice and water crushed ice just covered with water are required for this procedure Prepare both containers before starting the procedure A 1 Turn the unit on and enter the Configuration Calibration menu Allow the unit to warm up for 10 minutes Remove the end of the chamber thermocouple probe from the mounting tube near sample thermocouple connector inside the freezing chamber Dip the Chamber and Sample probes in the water crushed ice container and allow to stabilize for 5 minutes The probes must be in the center of the container well away from the container walls Observe the Chamber and Sample temperature displays Adjust the Chamber and Sample Offset parameters so that the displays each read 0 0 C Move the cursor to the proper parameter by using the Enter Back keys and change the parameter using the up down arrow keys Each degree or tenth of a degree of Offset will result in a respective change in the corresponding temperature display Dip the Chamber and Sample probes in the liquid nitrogen container and allow to stabilize for
73. edog e qonoj oq 03 JOY tas sjuouoduioo Jt p1eoq 1o 0 guoo oq Aeut rddns 1940d ooe dow z ISe guo umumndo 107 gy isnfpy 1 SAB dSTp omodu ur s1oquinu ostur Surdueqoun 10 pue we Aun oppT H SAAT ATN Ir possoud st Aoy UNY I uouA SIRIS Z o rjod 3os o1q pue eurrou 1eodde Ae dsrp amg1adua pue sc P180Q 1o o guoo 0 pojoouuoo SSQUIBY Jomod usyM A SLY UY sso OG AS H C j1senuoo GOT 831 JO 1ueunsnfpe AJIA ewou 1eodde S 47 pue s amp e dsrp omyexaduray JI T poq 1o oyuo p Aptdem GOT K ddns 140g z 1senuoo Kejdsip T I uoo1os use dgs dn 1940q ON A ddns 1amod q os e AG pieoq 1o o guoo ooe dow e K ddns 1amod ooe dow p Joxeo1q noro ae day p109 1omod ooe dow z uio qo1d poop 1 pieoq 19 o3juoo o pojoouuoo ssoumeu 1o od u ym juese1d Jou OqAZI Z1 S sindino Od s P180Q 1o o juoo WO p o uuoosrp ss u eu omod u uA J u s 1d jou DqACI cT S sindino DA y yndur A ddns Jamod 32 DVALOZ 06 Yey sse esnseojA JUL OV 1 TY ALOC 06 uet ss MSL c opno 1 2VALOC 06 uu Sse omseojq 1 peog onuo s K ddns 19mog Lr Jayeolg WMD p109 19 0q z jomo OV w 140g 1 Dom you ske dsIp pue s qHT uopnjos Uovo A Snep lqissoq uroyduri amp g opm Sunoougso qno Y 9 1 7007452 CryoMed Freezer Thermo Scientific pIpO0 1o o guoo ooe dow e
74. enu Entry D Time and Date Definitions co nt Select the parameter to change using the Back and Enter keys and change the selected parameter using the up down keys Exit this menu using the Back key The following parameters are set Day of the week Sun Mon Tue Wed Thu Fri Sat Month Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Date 01 31 Year 2000 2098 Hour 12 hour format 1 12 Minute 00 59 Second 00 59 e AM PM E Tracking Alarms Adjust the High and Low temperature tracking alarms for duration of run Time 1 minute increments and temperature difference from setpoint Delta 1 C increments The Time parameter can be adjusted over a range from a 1 to 99 minute range Exit the menu using the Back key 3 8 7007452 CryoMed Freezer Thermo Scientific Disinfect Controlled Rate Freezer Interior Thermo Scientific Section 4 Maintenance Warning Be sure to disconnect the unit from the power source before beginning any service work A Caution Before using any cleaning or decontamination method except those recommended by the manufacturer users must check with the manufacturer that the proposed method will not damage the equipment A Caution Do not use strong alkaline or caustic agents Stainless steel is corrosion resistant not corrosion proof Do not use a solution of sodium hypochlorite bleach as it can cause pitting and rusting A Warning Alcohol even a
75. er operating temperature range Note Rates vary based on load LN pressure unit size and voltage A Chamber Warming Rate Minimum of 0 1 C min no load couple in free air center of chamber Maximum of 12 0 C min same conditions as above Average of 9 0 C min same conditions as above range 40 C to 12 C Note Rates vary based on load LN pressure unit size and voltage A 6 2 7007452 CryoMed Freezer Thermo Scientific Thermo Scientific Section 6 Specifications LN Injection Patented liquid refrigerant injection ring optimizes uniformity and air flow Patent 6 044 648 Operating Environment Ambient Temperature 15 C to 35 C Relative Humidity 20 to 85 non condensing Insulation Foamed in place polyurethane Air Flow Horizontal from right to left as viewed from the front Sound Level Unit in operation does not exceed 65 dBA Operational Features Door interlock switch turns off LN2 heater and fan motor except during the warm up cycle During the warm up cycle the door can be opened with heater and fan running up to 0 C to accelerate warming LN pressure relief valve located adjacent to LN connection Chamber pressure vent port located on left side wall Heater overtemperature protection At a temperature level of 93 C a manual reset device interrupts power to the heating element External Connections Power entry Strip chart temperature recorder PC interface port LN2 co
76. er s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description Controlled Rate Freezer Product Designations 7454 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level IREL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC LVD 7323 EEC This product conforms to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 UL 61010A 1 Richard L Miller COE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Meclaratioa af Contormity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description IVF Controlled Rate Freezer Product Designations 7456 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC MDD 93 42 EEC This product conforms to the following Harmonized International and Nationai Standards EMC MDD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C222 No 1010 1 UL 61010A 1 Richard L Miller COE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 AA 2 7007452 CryoMed Freezer
77. er to the desired point on the graph Graph legend selection box includes the legend which shows the colors plot styles of the on screen plot lines A variety of colors styles may be selected for the current window only The default colors are listed in the Options Plot Colors window Any changes made are in effect for the current window only To change the color pattern click the plot line to be changed within the graph legend box Select the Color Line Style or Line Width from the pull down menu An on screen plot line may be rendered invisible by selecting a color that matches the on screen background color A white color will make the plot line invisible on color print out Scroll the Graph from side to side using the right left arrow buttons Use the up down arrow buttons to scroll the graph top to bottom Turn Auto Scroll off to view a portion of the graph off screen from the current plot position Thermo Scientific Status Indicators Operating Indicators Thermo Scientific Section 10 PC Interface Software The Controlled Rate Freezer remote status indicator messages are listed and defined below The indicator status is valid 1 5 seconds after the Run screen opens CRF Idle Freezer is in the Idle state CRF Warming Freezer is in the Warm mode CRF Busy Run screen was opened for a freezer currently running a stand alone profile Remote Mode Freezer is running under control of the PC CRF Running Stand alo
78. et added password and data tag info CCS 21878 FR 1735 9 16 03 Replaced P N 103113 with 195986 195987 195988 SRO CCS 5 21196 FR 1670 1 20 03 Add bend to bottom of probe guide tube CCS 21141 FR 1670 1 20 03 Removed 4000714 kit added 180254 cane holder and 4000211 cane CCS 21280 FR 1670 1 20 03 Changed bottom hinge block to 190612 for baseline alignment CCS 4 21059 FR 1642 10 9 02 Changed hinge block to 1900179 for better door alignment CCS 6 14 02 Added power disconnect warning section 4 1 per S Neill aks 3 5 15 02 Received FDA clearance for use in human research CCS 4 12 02 Added weight chart and new declarations of conformity for IVF units CCS 20558 FR 1582 3 18 02 Updated schematics and wiring diagrams for external IVF connector probe CCS 2 20604 FR 1592 3 12 02 Updated schematics and wiring diagrams aks 12 21 01 Added IVF models CCS 1 11 12 01 Updated profiles CCS 20382 FR1555 11 13 01 Change hinge mounting screws for freezer door aks 0 200164 S1 8280 8 23 01 Original manual CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific 7007452 CryoMed Freezer Preface CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance A Caution All internal adjustments and maintenance must be performed by
79. eturn to previously saved changes Click Exit to exit the Options screen Plot Colors Information Fields Default Directories Save Restore Select which of the Ileen available mlcemation helds displapable by the Run and Review modules are to used by clicking the check Box next to the field Then enter the label to be used for the field ii Fildt SswplelD Y Field 5 Meda ER Field 9 Ciyopvotectont ER Field 2 Sample Size E Field 6 it of Samples F Faek 10 D Feld 3 EI Field 7 P 411 Field 4 E Feld 8 Ti eld Ti Directories may be set up to store program profiles and run files Save before leaving the Default Directories screen Enter the desired path in the window for the default program directory and run data directory A Windows directory browse button may also be selected Click Save to keep changes Click Restore to return to previously saved changes Click Exit to exit Options screen CHF Configuration Options Plot Colors Information Fields Default Directories Users Save I L f Restore Select the default directories for locating freezer programs and run data Type the path in the Restore boxes below or click the browse button at the end of the bos ta select from a directory tree Exi Default Program Dieren C AThermoFormaVPrograms Default Run Director 4 C ThermoForma Run Files im Thermo Scientific Section 10 PC Interface Software User Name and Password User names and pas
80. g 7007452 CryoMed Freezer Alarm During a Run when the chamber temperature is higher or lower than the programmed temperature by a pre determined amount for a pre determined time period as set in Section 3 the controller will Sound a tracking alarm Light the Alarm LED Sound the audible alarm at a 7596 On duty cycle 2 second period Show Temperature is High or Temperature is Low depending on conditions compared to the programmed temperature on the fourth line of the display alternated with the normal fourth line display contents at a 2 second rate Mark the strip chart recorder and thermal printer at 50 C to document on paper where the alarm occurred Continue the profile run without interruption This is a self clearing alarm When in the alarm condition the audible alarm can be silenced by one depression of the Silence key but the Alarm LED will continue to flash and the alarm message be displayed while the alarm condition is present When the alarm condition is resolved the Alarm LED will stop flashing and the audible alarm will silence if the Silence key has not already been pressed However the alarm message will remain on the fourth display line until the Silence key is pressed a second time Thermo Scientific Section 2 Set Up and Operation Power Failure The controller will detect a power failure and alert the operator under the following conditions 1 Power failure less than 10 seconds i
81. hen the unit powers back up sim will be appended to the software version number on the initial power on display of the 4 X 20 LCD The simulation mode is disabled by changing the sim parameter from 1 to 0 exiting the Simulation menu and cycling the AC power Off On sim will no longer be appended to the software version number on the initial power on display Software Checksum This menu item initiates a checksum calculation of the software in controller memory and compares it to the reference value loaded in the controller when the software was installed In addition the checksum value is shown in the lower right corner of the display Test RAM This menu item initiates a non destructive test of all RAM on the controller board S RAM BAT RAM Any errors found would be reported on the LCD display i e Memory failure check UX Thermal Controller Adjust the cycle period of the thermal controller and integral proportional differential gain of the PI D temperature controller the minimum solenoid on time the temperature difference from the setpoint that the Cool solenoid is actuated and the difference in PI D parameters for the heater vs LN2 injection cooling Exit this menu using the Back key There is a separate set of parameters for each size of chamber available L 1 7 cu ft M 1 2 cu ft and S 0 6 cu ft Thermo Scientific Section 3 Configuration Calibration Menu Entry The following is a li
82. if password security is not enabled Run window status bar prior to the start of a run shows the communication port the profile file name and the path of the profile selected After the start of a run the bar shows the communication port the profile name and the run data storage file name The Start button initiates a Run This button changes to Move to during a Run when a different step is selected in the Step pull down menu The Print button initiates a print out during a Run or after a Run is complete using the Windows default printer Color or monochrome output may be selected a If default printer is monochrome select Monochrome b If the default is color select Monochrome or Color Choosing Monochrome on a color printer uses marker line patterns to identify different lines on the graph i e Sample temperature Chamber temperature Profile etc If Color is selected with a monochrome printer colors are represented by shades of gray so some colors may not be clearly visible c Selecting Single page report prints the current Run screen Thermo Scientific User Control Definitions Thermo Scientific continued Section 10 PC Interface Software d Selecting Two page report prints an information sheet first which includes the following Profile name and path Run data file and path User name Date time User Information Field name contents Run start stop comments Profile li
83. igure 2 4 Print outs Thermo Scientific 7007452 CryoMed Freezer 2 11 Section 2 Set Up and Operation Alarms Alarm messages are shown on the fourth line of the LCD display Alarm messages alternate if multiple alarms are present at one time Alarms are cleared with the Silence key When an alarm message is shown the unit will alternate between the alarm message s and the normal fourth line display at a rate of 2 seconds per message Silence key First depression silences the audible alarm The second depression turns off the Alarm light and clears the message on the display if the alarm condition is resolved Chamber Thermocouple If a chamber thermocouple cannot be detected Open Audible alarm sounds continuously Alarm indicator lights Message C sensor fail shows on the LCD display ILL shows on the numeric display chamber Freezer switches to Idle Mode and operating functions are disabled The audible alarm is silenced by the first depression of the Silence key When the alarm condition has cleared another depression of the Silence key is necessary to turn off the Alarm LED and clear the alarm message from the fourth line of the display The numeric display message will revert to a normal temperature display when the alarm condition clears 2 12 7007452 CryoMed Freezer Thermo Scientific Section 2 Set Up and Operation Sample Thermocouple The freezer responds to an open Sample thermocouple in different wa
84. ile 2 Commonly used for very small 96 well sample size resulting in a 1 C rate from nucleation to 40 C and a 10 C per minute cooling rate to a 90 C end temperature Step de sa tasata au eode da ate a devin Petia Wait at 4 0C SEP 2e n eS E quero iinet hese ede 1 0 C m S to 40C KEE 10 0 C m C to 90C SEPA nini a EER O emus End 1 4 7007452 CryoMed Freezer Thermo Scientific Pre set Profiles continued Section 1 Introduction Pre Set Profile 43 Commonly used for straw size samples resulting in a slow rate to 7 C a 5 minute hold for manual cell nucleation a 0 3 C rate to 35 C and then a 0 1 C per minute cooling rate to a 37 C end temperature Step BEES Wait E o i 1 0 C m C MEDIA A pH d 0 5 C m C Step ap vues Pu eq EAE Hold for 5m GER ac A ISA P du d RA 0 3 C m C Step vaga iss yn ERA UR adhe sa ias 0 1 C m C SUED re EE ALIAE Ae Vd S End at 20C to 4 0C to 7 0C at 7 0C to 35C to 37C Pre Set Profile 44 Commonly used for hemopoietic stem cells that are 65 100ml in size resulting in a 1 C rate from nucleation to 45 C and a 10 C per minute cooling rate to a 90 C end temperature SU NEE do ite cott a itd stan calcium os As Wait KEE 1 0 C m S K s ap vip Us qu Ee EN ES 25 0 C m C DEED Ee Are 10 0 C m C Step EE 1 0 C m C Step i pie E o Lata Ld M Lut 10 0 C m C Step 75 ses ve AA RN URNA End at 20 0C to 6 0C to 50C to 14 0C to 45C to 90C Pre Set Profile 45 Commonly used for
85. int Button This button is used to initiate a print out of the Review and Compare data using the Windows default printer Select color or monochrome black amp white output If the printer is monochrome select gt monochrome If color select either Monochrome uses marker line patterns to identify specific lines on the graph If Color is selected with a monochrome printer colors are represented by shades of grey some colors may not be clearly visible 10 20 7007452 CryoMed Freezer Thermo Scientific User Controls continued Thermo Scientific Section 10 PC Interface Software Single Page Report may be selected to print the current Review screen Iwo Page Report may chosen to print in addition to the current view of the graph one or two information sheets The first sheet printed on a two page report is the open Review file information 1 2 3 4 5 6 7 8 Review file profile name and path Review Run data file and path User name Date time User Information Field name contents Run start stop comments Profile listing Alarm listing with time of occurrence If a Compare file is open a second information sheet is printed Multi page Report prints information pages on the Review profile and the Compare profile if open Subsequent pages are the entire Run graph Horizontal scale is as selected on the Review screen This also determines the number of pages as indicate
86. isplayed Sample probe temperature shift A If the temperature of the Chamber or Sample probe reaches the chamber design high or low temperature limit design temperature limits are factory pre set at 55 C and 185 C the controller will 1 Sound a Temperature Limit alarm 2 Light the Alarm LED 3 Sound the audible alarm at a 75 on duty cycle 2 second period 4 Show C temp limit or S temp limit on the fourth line of the display depending on which probe is at or exceeding the design temperature limit alternated with normal fourth line display contents at a 2 second rate 5 Continue the profile run without interruption This is a self clearing alarm While in the alarm condition the audible alarm can be silenced by one depression of the Silence key but the Alarm LED continues to flash and the alarm message displays as long as the alarm condition is present When the chamber temperature returns to a value within the pre set limits the Alarm LED stops flashing and the audible alarm mutes if not already muted by pressing the Silence key However the alarm message remains on the fourth display line until the Silence key is depressed If the temperature of either probe continuously exceeds the pre set limit temperatures for more than 10 minutes the unit will immediately switch to Idle Mode and disable all functions 7007452 CryoMed Freezer 2 15 Section 2 Set Up and Operation 2 16 Temperature Trackin
87. lay for profile status messages and freezing profile editing and viewing Thermo Scientific Thermo Scientific Indicators Keys Alarm Output Section 1 Introduction At the top of the control panel are two columns of LED Operating Mode indicators The left indicators are e EE No profile is running EEN A profile is in progress Wait Profile in progress pauses until the Run key is pressed End AA hd Geeta arpa ona Profile is complete ic EE Chamber is warming to 25 C The right column of indicators are Cool isc alin eg U Single LN solenoid is open T i1 162i Both LN solenoids are open Heated nite ein The heater is energized There are seven input keys on the control panel SMOD ates eeu ds ede Starts the warming cycle BUN Gad te See ARRAS Starts chosen profile or resumes after Wait Silence rz Honesto Turns off the audible alarm and clears the alarm messages from the display A Back suasana ER Terminates a profile in progress returns to Program menu or moves the cursor to the left b Enteri emi ha l saya Starts a new line enters a sub menu moves the cursor to the right or enters View or Edit Mode BAUD TT Scrolls upward through display lines or changes profile step and set up parameters w Down Scrolls downward through display lines or changes profile step and set up parameters Above the Silence key is the alarm indicator In an alarm condition the LED flashes in addition to
88. lder coo Uo ota oco io oL 2 20 Holder Adj stment sspe Eet oe ELSE Mac E 2 21 7007452 CryoMed Freezer V Table of Contents Section3 Configuration Calibration 3 1 Ment Structure e ad cR te TT 3 1 Menu Entry Definitions 3 1 Section4 Maintenance 4 1 Disinfect Controlled Rate Freezer Interior o oooooo 4 1 Clean a Major Spill in the Chamber 4 2 Clean the Cabinet Exterior 4 3 Clean Gasket Heat Break 4 3 Section5 Printer Set Up 5 1 Change the Papers raar E a risa da Vitas aqu R sa 5 1 Section6 Specifications 6 1 Section 7 Paris Lisa ia eret aie tah tax gras EE E states he 7 1 Exploded Parts Drawings o i aswell ue udi seu ety ee ay 7 3 Witing Diagrams Z paus eed tr M a MEA pA Ss 7 15 Section8 Electrical Schematics 8 1 Section9 Troubleshooting 9 1 Section 10 PC Interface Software 10 1 Installation and Set Up 10 2 Man Menuires anta eA kuy Eh Laos ayy d 10 2 EEN 10 3 Create a New Freezer Profile 00 c cece eee 10 7 Edit an Existing Profile Sek E Zeg esd A Eege es 10 8 Print Profile Li
89. lder warmer than the sample itself A Thermal controllers are limited to 55 C and 185 C during steep Sample ramps Note For chamber ramp steps the Internal ramp interior set temperature will ramp T factory set at 1 C beyond the programmed 1 C over chamber setpoint Chamber temperature setpoint temperature to assure that the ramp end point condition will be reached under all operating conditions For example if the chamber set point is 30 C the controller will ramp to 31 C until the setpoint is reached Graph print outs show this ramp setting A Figure 2 4 Ramp Settings Thermo Scientific 7007452 CryoMed Freezer 2 9 Section 2 Set Up and Operation Program User Specific Profiles cont Copy and Paste Pre set and User Profiles 2 10 7007452 CryoMed Freezer Wait at 0 0C Go to specified chamber temperature and wait until the Run key is pressed Hold for 1m ar 0 0C Hold for specified time at noted chamber tem perature then continue A double beep will sound at the end of the hold time Precool Cool chamber w target of 4 C and continue to next step when a sample temperature of 5 C is reached Jump to 1 Jump to a specified step Loop to 1 for 1 Loop to step and repeat specified number of times End End of profile 9 After deciding on a function command press Enter to move to the temperature time or step number to be set Scroll using the 5 6 keys to choose a number
90. lete button e Exit Current Users gt User Name Add Password Delete Confirm Password Thermo Scientific 7007452 CryoMed Freezer 10 25 Section 10 PC Interface Software 10 26 Saving Changes Sharing Host PC Resources 7007452 CryoMed Freezer Each of the four screens described above must be saved individually The application will prompt to Save if unsaved changes are present when the Exit button is clicked When using the same PC for several freezers potential problems could occur during printing There could be a loss of Run data points Chart data is updated every 6 seconds Run data is captured every 6 seconds with updates to hard disk every minute Serial port communication may stop for a short time during printing The loss of a small number of data points during slow ramps or holds will not cause a problem chart lines are interpolated across the gaps Loss of data during fast ramps may be more critical Experiment with test runs before trying to print during a critical part of a critical freeze If in doubt don t print during a critical run If because of printing the freezer loses communication for more than 20 seconds the freezer will go in Stand Alone mode A very slow PC lt 166MHz with limited resources lt 32MB RAM may stop serial communication for more than 20 sec during printing In Stand Alone mode PC control is disabled and there may be loss of run data for the dura
91. ll store at least 2 hours of data A d Select Printer Temperature Scale Select one of two printer scales 180C to 50C default or 100C to 50C e Select Printer Time Scale Select 1 2 or 5 minutes per division 2 Probe Calibration Allows operator to calibrate the Chamber or Sample probes by entering an offset and scale factor scaler Exit this menu by using the Back key Use an appropriate reference thermometer for comparison near 0 C offset and 196 C scaler Note The chamber sample limit temperature alarm will sound during the scale factor adjustment at 196 C Silence the alarm during the calibration procedure by pressing the Silence key Clear the alarm message after the procedure is complete by pressing the Silence key one more time A Important After calibrating the temperature probe reinstall the probe in the previous position Make sure the tape on the probe wire is flush with the frame for consistent probe results A Figure 3 1 Install Probe 1 Probe Offset The Chamber and Sample probe offset can be adjusted over a range of 9 9 C to 9 9 C in 0 1 C increments Adjust the probe offset for a 0 0 C temperature display reading with the probe in a standard crushed ice bath 7007452 CryoMed Freezer 3 3 Section 3 Configuration Calibration 34 Menu Entry Definitions cont 7007452 CryoMed Freezer 2 Probe Scaler The Chamber and Sample probe scale factor scaler can be adjust
92. mo Scientific 7007452 CryoMed Freezer 94 K1essooou s soqo1d ojeqi eo 31 JO S3 ngJop K10329 01 19521 0 Jord songea uonelqi eo oqoud JO ou IJEN nuoui 2IA19S ou ur si nejJ p 1039e 01 JOSOI JO son eA JId 199009 199 uonisod peoy o dnooouuoq 3snfpy Z Joje ngo1 yue ooejdow I uonisod Ajr19A pu JOYS 103 389 L LE nuou Io O 3u0 Ieuu u IMAS ur S n 8A CL 3994100 AJA your f 9qm Sununoui jo mo peou o dnooouuog AJA 17 Teururou 77 10ye n391 ounssoud yue Ajo A T peuonisod Kpooxioour 10 p lious D L p SJojoure1ed Gd poisnfpe 1ou1ojsn Sununour e dnooounog Joquieyy 7 omssoid NT yY31H T aeo onje1oduro quey opm Sunooqgso qnoa Y 9 5 7007452 CryoMed Freezer Thermo Scientific Thermo Scientific Section 10 PC Interface Software Section 10 PC Interface Software la Cnr PC Interface Controlled Rate Freezing P Interface for GryoMed Freezers Figure 10 1 Opening Screen The CryoMed Controlled Rate Freezer CRF includes the option for PC interface The interface requires one of the following operating systems MS Windows 95 98 ME NT XP or 2000 The PC software can create and download freezer profiles It can initiate a profile run monitor its progress then create a printed report summary of the run data During the time that the freezer is running a PC initiated profil
93. n duration Continue profile run without interruption or alarm after power is restored 2 Power failure more than 10 seconds in duration Flash Alarm LED and sound audible alarm at 2596 On duty cycle with 2 second period Show duration of failure and Power Failure Alarm on second third and fourth line display display line alternated with normal fourth line display Mark strip chart recorder and thermal printer re print temperature scale on thermal printer Continue profile run without interruption A single depression of the Silence key will clear the audible alarm A second depression of the Silence key will clear the Alarm LED and the messages on the second third and fourth display lines Run Complete Five minutes after the End profile step is reached the controller will sound the Run Complete alarm Flash Alarm LED and audible tone at a 2596 On duty cycle 2 second period Show End of run on the fourth line of the display The controller will maintain the temperature of the final step in the profile run until the run is stopped by the operator pressing the Back key Caution If performing successive runs dry the gaskets and heat breaks between each run A Thermo Scientific 7007452 CryoMed Freezer 2 17 Section 2 Set Up and Operation Warm Complete Seeding Hold Printer Buffer Full IVF Sample Holders 2 18 7007452 CryoMed Freezer Five minutes after the chamber reaches 25 C during a Warm
94. ne Freezer started a Remote Run under PC control but is now running the remote profile off line This is caused by a PC to CRF communication interruption of more than 20 seconds The current Chamber Sample temperature indicators show the present temperatures at the freezer ILL Indicates an open Sample or Chamber thermocouple probe The Operating Status indicators are as follows e Idle CRF is in idle mode fan solenoids and heater turned off Run Running a profile Wait Running a profile but waiting for user intervention End Running a profile but at the End last step in the profile Warm Warming the chamber to 25C Pop up status windows display a message requiring user intervention During a profile Wait step a message will appear Program waiting for user Click here to continue Click on the message box to continue to the next step in the profile Alarm messages may also appear before or during a run These messages alert the user to the alarm status of the freezer The user must clear the message on the screen even though the alarm condition may have already corrected itself 7007452 CryoMed Freezer 10 13 Section 10 PC Interface Software Operating Indicators continued 10 14 7007452 CryoMed Freezer For more information on the alarms listed below see the Controlled Rate Freezer manual Temperature High tracking Temperature Low tracking Power Fail and failure duration
95. nnection 1 2 flare External thermocouple jack IVF models only Sample access IVF models only 7007452 CryoMed Freezer 6 3 Section7 Parts List 201183 201185 370248 20V Printer Power S 00V Printer Power S 230V Printer Power S 20V Printer Power S 5A DPDT Circuit Bre 8A DPDT Circuit Brea CIC CES upply upply upply upply Cord aker Switch er Switch 4 MPT Relief Valve 60 PSIG T Type Chamber Probe T C Plug Type T 370249 T C Socket Type T 4000385 Internal Sample Probe 1 2 ml 4000386 Internal Sample Probe 4 5 ml Thermo Scientific Cordset 13A 125V 16 3 USA 301 601 13 4 52 8 6 7007452 CryoMed Freezer 74 Section 7 Parts List 7 2 7007452 CryoMed Freezer IVF Models 0nly L Thermo Scientific Section 7 Parts List e PART s d DELE DOOR ASSEMBLY 7452 200 1 B REV PAGE 1 DF 2 Thermo Scientific 7007452 CryoMed Freezer 7 3 Section 7 Parts List BILL OF MATERIALS ITEM PART ND PART DESCRIPTIUN QTY 22008 486 32 X 3 4 SS PHP SCREW 2 22051 488 32 X 1 4 SS PHP SCREW 2 74 N N gt p S OOO LH N l 2 3 8 32 X 3 8 SS PHP SCREW 24 4 22053 88 32 X 1 2 SS PHP
96. o clean between the sample access openings on the top of the unit A Dry any moisture from the door gasket and heat breaks between each run using a soft cloth to prevent ice build up which could reduce the effectiveness of the door seal 7007452 CryoMed Freezer 4 3 Section 5 Printer Set Up The built in thermal printer has internal software switch settings that control how the printer interfaces with the Controlled Rate Freezer These internal settings are pre set at the factory for proper operation with the freezer However if the printer has to be replaced in the field the settings must be changed to interface correctly with the freezer The following chart shows the parameters which may be set the printer manufacturer default settings and the settings required by the Controlled Rate Freezer See the included printer manual for the software switch setting procedure Change the Paper 1 Thermo Scientific Turn the unit on Trim the paper as needed to make edge straight paper Open the printer cover Feed the paper into the inlet at the bottom of the paper holder until the auto loader begins to pull the paper in The auto loader will feed about 4 inches 10cm through the paper cutter Press the paper feed switch until the paper serion feeds in straight and smoothly Slot The printing surface is the outside of the thermal paper roll 7007452 CryoMed Freezer 5 1 Section 5 Printer Set Up 5 2 7007452 CryoMed F
97. of or recycled through them Further information on Thermos compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermo com Y Always use the proper protective equipment clothing gloves goggles etc Y Always dissipate extreme cold or heat and wear protective clothing v Always follow good hygiene practices v Each individual is responsible for his or her own safety Thermo Scientific 7007452 CryoMed Freezer iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 740 373 4189 FAX http www thermo com Internet Worldwide Web Home Page service led marietta thermofisher com Service E Mail Address Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended
98. ol solenoids on 7007452 CryoMed Freezer 10 11 Section 10 PC Interface Software User Control Definitions 10 12 continued 7007452 CryoMed Freezer Time Temperature Division pull down menus are as follows Temperature divisions may be chosen by clicking the down arrow to select 20 10 5 2 1 C per division b Time divisions may be chosen by clicking the down arrow to select 20 10 5 2 1 0 5 0 1 minutes per division The Program Listing pull down menu shows all the steps in the current profile A check mark indicates the step that is currently running This menu can be used to manually move the freezer to a different step forward or backward Select the step destination It turns yellow to indicate the change is not yet complete When a different step is selected the Start button changes to Move to Select it to execute the move The selected step turns white to show the move is complete and the new step is running To start a Run at any step after the first step choose the step to begin Then select the Start button to begin the run The step turns white to show the change is complete The Run starts at the chosen step Chamber Sample temperature window indicates these temperatures at a chosen point on the graph To choose a point on the graph first place the mouse pointer on the default cursor position left side of the on screen graph yellow vertical line Press and hold the left mouse button and drag the curs
99. ontrolled Rate Freezer Product Designations 7451 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC LVD 73 23 EEC This product conforms to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 Decloraiinn uf Contormity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufacturer s Address 401 Millcreek Road Marietta Ohio 45750 U S A Product Description Controlled Rate Freezer Product Designations 7453 Year of Initial Marking CE 2001 Affected Units Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC 89 336 EEC LVD 73 23 EEC This product conforms to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 Richard L Miller CQE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 7007452 CryoMed Freezer AA 1 Declaration of Contormity Manufacturer s Name Thermo Fisher Scientific Asheville LLC Manufactur
100. oss a matrix of 10 vials 1 2 2 0ml size containing 1 0ml 2 each of distilled H20 The temperature is to be monitored by type I thermocouples placed in the center of the liquid in each vial with the vials distributed in standard racks and holders as shown below separately for each cabinet size Please note that there are 5 each vials in the second rack from the top as well as the second rack up from the bottom with the Sample vial placed in the center of the top rack Also note that the large cabinet uses a combination of the small and mid size rack and holder sets Finally a specific program sequence listed below must be run in order to collect the uniformity data Rack and Holder Vial Placement for each size chamber Models Models 7450 7451 7452 7453 7454 7455 7456 7457 7458 7459 7474 7472 7475 7473 7476 7007452 CryoMed Freezer 6 1 Section 6 Specifications Uniformity Profile 1 Hold 10 0min 225C 2 2 0C min C to 0 0C 7450 140 63 5 3 0 0C min S to 3 0C 7451 140 63 5 4 Hold 10 0min 1 0C 7452 160 72 6 5 1 0C min C to 5 0C 7453 160 72 6 7454 7455 180 81 6 180 81 6 7456 140 63 5 7457 140 63 5 9 Hold 12 0 min 2 0C 10 1 0C min C to 20C 1 END Chamber Temperature Range 50 C to 180 C 6 99C min C to 50C 7 99C min C to 10 0C 8 1 0C min C to 2 0C Chamber Freezing Rate Minimum 0 1 C min Maximum 99 9 C min ov
101. ou a T while pressing the down arrow key gives you a gt A At this time enter a unique name or title for the profile When Step 0 the name or title is complete move the cursor back to the leftmost position This is View Mode Scroll using the down arrow key to Step 1 Press Enter to go into Edit Mode Step 1 contains a command with a cursor blinking just to the right of a left pointing arrow JL With the cursor in this position use the up or down arrow keys to scroll through the available commands Thermo Scientific Section 2 Set Up and Operation Program User Specific Listed below are the available step types Profiles cont 0 0 C m C to 0 0C Ramp chamber temperature at the specified rate until the chamber reaches the specified temperature 0 0 C mS to 0 0C Ramp chamber temperature at the specified rate until the sample reaches the specified temperature For this particular function when the cursor is in the tens position pressing the up or down arrow Ramp Function keys changes the available function The cursor Number Position must be in the units position to change the rate value See Figure 2 3 for position location E The monitored trip temperature either S for 00 0 Sample or C for chamber can be changed by plac Figure 2 3 Cursor ing the cursor on the S or C and using the up or Positions down arrow keys Caution Steep ramp rates may cause chamber to be much co
102. pnjejeo sjenueuJ uononjjsur paud SeAuJe jueuudinbe jno eJojeq uoneuuojJur uoneJedoeJd ays aasuasyalduos ym diau oj pea si SOO sejes ouu u 187201 INOA Ssjonpoud JO ssoj Jo SHJOAIA 1901 0 Bunejas soBewep uoneyu JjnouliA Bulpnjoul sabewep jenuenbesuoo Jo yapu Aue 10J ejqei eq jou UEUS oway 82UEUOS UIEUJ 9AI USA 1d pue uoneJedo uone ejsur 1ueuudinbe jleyep Ajjnjoseo sjenueuu uononujsui paud 061 LC LZ 999 L Peau Og pue J9Z99J4 91 p ljojuooS eui uo suonsenb jeuoneJjedo pue dn es JO pejoejuoo eq Aew juauyiedag sexinas esluyos eu pepeeu se way 19ejuoo esee d JOINJOPJNUCLW jueuoduJoo ay o paJuaje eq 1snuJ sjueuoduJoo JejnduJoo y jo Aue uo AjueueM SU L ALNVAYVM STVWY43Hdl43d ANV d aJ 1Va3a TVNOILLVNY43 _L1NI OIdILN3I9S H3HSI3 ONY3H_L Thermo Scientific 7007452 CryoMed Freezer 11 2 re EIE DEHI 1006 OSI 0 2 2 NY uoneuuJoJur AUS UE JO JOJNQUISIP e20 Jno j9ejuos wen 34 apisimo uoneordde jeloads pue s s eoueuojureui uoneJedo AE UE 1ueuudinbe uo suonsanb noA Jamsue o peaJ 949M 9 t5 0v4 L Jo epeue pue YSN 064 1 1Z 888 1 1e juauyuedag samas eoiuuo9e JINOA jeo aseajd peuinbaeJ s sones 1ueuudinbe J eoueuojuleuJ 9AnueAaeJd pue uoneJedo uonej ejsur 3ueuudinbeo reyep Ajjnjoseo sjenuew BuneJedo pejuug seAuue jueuudinbe noA aJojaeq uomneuuojur uonejedaJd ous eAisueueJduioo YM diau oj peai si BdIYO sejes ouueu 18701 INOA SjonpouJd JO sso JO SHJOJA
103. r Do not over tighten when connecting Internal plumbing could be damaged Teflon tape is NOT recommended when installing LN supply The tape cannot withstand the low temperatures of this application See Figure 2 1 for the connection location Refer to the electrical schematics or serial data tag on the unit for electrical specifications for the Controlled Rate Freezer 1 Verify the power switch on the back of the unit is off 2 Attach the power cord to the inlet on the back of the freezer Connect the power cord to the appropriate power outlet The Controlled Rate Freezer is designed to be electrically safe in the fol lowing environmental conditions Indoors Altitude Up to 2 000 meters Temperature 5 C to 40 C Humidity 80 RH at or below 31 C decreasing linearly to 50 RH at 40 C Mains Supply Fluctuations 10 of nominal Installation Category II Pollution Degree 2 Class of Equipment I 1 Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V
104. re1qi e uorjenaguo up y Jopioosw p IJopJ0oo1 Je juosoJd 100 JJ p1eoq J9 OJJUO9 uo 101990003 19p10991 JIBUYO W ALG xoidde ounseaui pue 2 c 10 Iop10201 jos NUS Jop1ooo UE uore1qi e uoreansguo up e uoroeuuoo o qeo 1op1ooow I JJO 19 199 NUDUI Top1o2o3T Heyo uone1qme uonemsuo yoy c dnjos nuour 1 p1099 17 post 1431 19MOd 19p10991 uu1guo LT Jomod 1opioooq T jndjno J9p10991 ON SOYSUMS qq Wud yasoy p A ddns Jomod 1ojurid ooe dos eg ourgoeur Jo 16291 pieAo uo yams Jomod um EI Jo o dureg r qureuO PAS 5 Iojurid oove do1 10 wef mear qq Joded Sua ooejdo eq Jojurid uo uoynq ouuo SSA T preoq 1o o3uo 9 ssoureq Jam e uo3nq ouiqu Surp ouy oym dn 1940d PAOI pu Z I MS dIG 1o1uuid uunguo y dnjos 1ojurid yeuroyuy p 5dAS 9 1032ouuoo ISA 0d 1ojuud 3e ISPIOA JIN BE UO SI 19 ULIO JO opis Yo uo YIJIMS TIMO uuguo guud Jo zuo uo TT 1940d oou E Jomod ruud JFO OF 198 nuou 19JULIJ uoneqi e uonens3rguo JIN c das nuour symud c ong pooj Surplou o rqa dn Jomod yud 3593 oq wel 1oded 101 yooyD jud uo PAYS SYST eurggo pue ouuo qT roded 19949 Suryseyy 1431 SUBO Ier Jojurid uo paJySt 1931 ouuo uoo18 uuuo LT 9UI Uo JOU 19104 T jndjno Joyurid ON uonnjos UODE2ULIS A ISNE o q1ss0q mode opm gunooqso qno Y Ther
105. ready existing profile may be changed as needed 1 Open a freezing profile by selecting the Open button The steps appear in the program listing window Select the step to edit Change the step type or modify the step parameters and click Replace Choose the point at which to insert a new step New steps are inserted at the position highlighted in the program listing window Subsequent steps move down one position as new ones are added Select the step type and enter the desired parameters Press the Insert button to insert the new step Use the Replace button to modify or edit a step Use the Delete button to delete a step or the Delete All button to delete all the steps in the profile Save the edited profile The same file name may be used or a new profile name can be entered An optional comment may be saved with the profile Comments are printed with the Run data A listing of the open freezing profiles may be printed at any time by selecting the Print button Print requests are sent to the Windows default printer To choose a different default printer first exit the interface software Choose the printer then restart the PC interface software The printer selection will not be changed if the software application is still open at the time of the change Thermo Scientific Section 10 PC Interface Software Display Profile Temp A representation of the freezing profile is displayed on the Edit window Graph graph Refer to
106. reezer Table 5 1 Internal Settings Parameter DIP SW 1 1 Input 2 Printing Speed 3 Auto paper loading 4 Auto line feed 5 DIP switch setting 6 Print density a 7 Print density b 8 Print density c DIP SW 2 1 Print mode 2 User font back up 3 Character type 4 Zero style 5 Character set a 6 Character set b 7 Character set c 8 Character set d DIP SW 3 1 Data length 2 Parity with without 3 Parity odd even 4 Busy control 5 Baud rate a 6 Baud rate b 7 Baud rate c 8 Baud rate d Default from manufacturer Required for CRF switch setting Parallel ON High ON Enabled ON Disabled OFF Enabled ON 10096 OFF ON ON switch setting 40 columns ON Enabled ON Normal ON Normal ON Japanese ON ON ON ON switch setting 8 Bits ON Without ON Odd ON Hardware ON 75bps ON ON ON ON switch setting Serial OFF High ON Enabled ON Disabled OFF Enabled ON 100 OFF ON ON switch setting 40 columns ON Enabled ON Normal ON Normal ON USA ON ON ON OFF switch setting 8 Bits ON Without ON Odd ON XON XOFF OFF 9600bps OFF ON ON ON Thermo Scientific Thermo Scientific Section 6 Specifications Temperature Uniformity Temperature uniformity is specified to be less than a standard deviation of 2 C up to the freeze of the first vial acr
107. rmo Scientific Using a Vial Holder Using the Straw Holder Thermo Scientific Section 2 Set Up and Operation The vial sample holder secures a vial for insertion through the access port on the top of the IVF freezer This holder may also be used to hold the external sample probe See Figure 2 5 Press 1 To secure the vial first make sure the button holder is in an upright vertical Press a position 2 Press the button on the top of the holder This temporarily widens the Side View opening at the bottom of the holder to install the vial 3 Carefully let the button up on the top of the holder while making sure the vial fits into the indentation of the holder The vial should be secure in the holder when the button is released Moves with button Fit vial into 4 If the holder is securing the sample asrbe probe vial hole in vial cap slide the below probe down through the holder until it feeds through the vial cap Figure 2 5 Vial Holder 5 Install the other end of the probe to the external connection on the top of the unit See Figure 1 2 The straw sample holder secures up to 4 straws for insertion through the access port on the top of the IVF freezer The straw holder may also be used to hold the external sample probe See Figure 2 6 1 To secure the straws first make sure the holder is in an upright vertical position 2 Press the button on the top of the holder This temporarily widen
108. rsor press and hold the left mouse button and drag the cursor to the desired graph position Graph color selection box This box includes a graph legend showing the colors plot styles of on screen plot lines Select from a variety of colors styles for the current window only Default colors are as specified in the Options Plot Colors window Any color pattern changes are in effect for the current window To choose colors patterns left click the plot line to be changed within the legend box Select the color line style or line width from the pull down menu Selection of an on screen background color can make the plot line invisible on the graph White can be selected to make the plot line invisible on print out color print out Graph Scroll Buttons The left right scroll arrow buttons move the graph right or left in the display window The up down scroll arrow buttons move the graph up or down in the display window Thermo Scientific Section 10 PC Interface Software Options Several options are available See the tabs in the illustration below Plot Color Selections The plot colors must be chosen so as to be distinguishable from each other on the screen and in the print outs Make sure there is a noticeable difference between the Chamber and Sample colors Choose plot colors for the Chamber temp Sample temp Profile Alarm steps background and grid Save before leaving the Options screen To choose colors click on the color
109. run However Access Code protection is not required on an End step If a power outage interrupts a run the profile will restart from the point of interruption when power is restored See Alarms in this section Thermo Scientific Manual Override Program User Specific Profiles Thermo Scientific Section 2 Set Up and Operation The user may override a running freezing profile by manually injecting LN or operating the heater to more rapidly change the chamber temperature 1 During a running profile press the Enter key to access Manual Override Mode If Access Code protection has been enabled the unit will prompt for the access code before entering Manual Override Mode 2 Select the desired function to override Heat Cool or Cool by pressing the Enter and Back keys Heat controls the heater Cool energizes the solenoid Cool energizes both solenoids 3 Press and hold the up arrow key to turn On the selected function or press and hold the down arrow key to turn it Off The function will revert to normal profile control when the key is released Note The function not under manual control will still be under profile control For example if the Cool function is activated under manual override and the chamber drops below the programmed setpoint the controller will activate the Heat function to compensate A To program user specific profiles enter Edit Mode The following describes the actions and options necessary for
110. s When power is restored to the PC and Windows reboots restart the freezer application Select Run from the Main Menu Choose the COM port for the freezer that is running The PC will recover the Run data captured prior to its power failure then continue the Run in Stand alone mode The graph on the Run screen will show the gap when the PC was not communicating Data not saved prior to the power failure cannot be recovered It could be as much as 1 minute of Run data as the application saves data to disk approximately once per minute Up to four Run screens can be restored for freezers that were running during the PC power failure As the freezer did not experience the power failure no alarm will be recorded If both the freezer and the PC have power failures see the previous paragraph The behavior and recovery are the same as above COMMUNICATION FAILURE EM FREEZER NOT RESPONDING Communication Failure CRF Not Responding This message indicates that the freezer is not sending status updates to the PC when requested Verify that the communication cable is connected and working correctly the freezer has power and is turned on and that there has not hardware failure in the PC or freezer If communication is interrupted for more than 20 seconds the freezer will go into Offline mode Freezer Already In Use Freezer Already In Use This message appears when a AE en i is selected for a freezer tha
111. s activated A B Print Error Logs This menu item prints and resets the diagnostic error logs that are stored in battery RAM Each error log consists of the alarm message and the time date of occurrence A maximum of 20 alarms for each alarm type are stored in the log Older entries are discarded when more than 20 alarms are accumulated The following is a list of alarm types stored Chamber Sample probe failure during a run Chamber Sample over under temperature limit Power failure also stores power failure duration Heater error Check LN2 supply RS 232 communication error Reset Error Logs This menu item is used to clear the Error Log entries When the log is empty this item reads Error Log Empty 7007452 CryoMed Freezer 3 5 Section 3 Configuration Calibration 3 6 Menu Entry Definitions cont 7007452 CryoMed Freezer D Simulation This menu item enables a thermal simulator that allows the unit to run freezing profiles in a realistic manner without actually using the chamber heater or cooling solenoids The simulator uses separate parameters for heating and cooling effects that approximate the performance of the real chamber The delays supported are Turn On delay Del Turn Off delay Eff Rate of temperature change per minute C M Simulation mode is enabled by changing the sim parameter from 0 to 1 exiting the Simulation menu and cycling the AC power Off On W
112. s the opening at the bottom of the holder to install the straws 3 Carefully let the button up on the top of the holder while making sure each straw fits into an indentation of the holder The straws should be secure in the holder when the button is released 4 To install the straw probe insert the probe down the center of the button indicated below Slide the probe down through the holder until it feeds through the center straw 7007452 CryoMed Freezer 2 19 Section 2 Set Up and Operation Using the Straw Holder 5 Install the other end of the probe to the external connection on the top continued of the unit See Figure 1 2 Press button Using the Cane Holder Side View Slide into slot to Ae Moves with button Fit straws into indentations up to 4 the center position is used for external sample probe Figure 2 6 Straw Holder Figure 2 7 Cane Holder The cane sample holder secures a cane for insertion through the access port on the top of the IVF freezer See Figure 2 7 To use the cane holder first assemble the aluminum holder to the plastic handle Insert the cane holder assembly through the access port on the top of the unit See Figure 1 2 If the cane holder is used with a sample probe use the standard 1 2ml vial probe Connect the probe to the internal sample probe connection Caution Do not connect two sample probes at the same time A 2 20 7007452 CryoMed Free
113. sting Alarm listing with time of occurrence The second page printed is the current screen graph display e Selecting Multi page report prints the information sheet described above as the first page The pages following are the entire Run graph printed vertically for best presentation Horizontal scale may be selected on the Run screen The number of pages to be printed in horizontal scale is indicated on the print out selection window Stop Exit button stops a Run in progress or exits the Run screen after a Run has ended The Run screen may be exited during Warm However the Controlled Rate Freezer will maintain Warm Mode until terminated by the user at the keypad Auto Scroll button is an On Off button with the default being On This button has an automatic adjustment of the graph scale so that it is always visible in the window In addition the most recent data is automatically displayed The Warm button appears when a Run is terminated It allows the user to initiate a Chamber Warm from the PC This button has an On or Off option Click once to turn On click again to turn Off Manual override buttons are activated by pressing and holding the mouse button When the mouse button is released the function is deactivated This button is only available during a profile Run a The override Heat button turns the heater on b The override Cool button turns the Cool solenoid on c The override Cool button turns the Cool and Co
114. sting asis iiu SOR as EE 10 8 Display Profile Temp Graphique tede Debe ri xS 10 9 Error Messages 025 ves vat ea de A i d 10 9 Run Screens e Mu xit eB estes eo AO R dele udine cafa O 10 10 User Control Definitions 10 10 Status T E Y pes ier aba EEN 10 13 Operating Indicators 10 13 Typical Run Procedure sarita roo rad upra 10 15 Power Failure Recovery 10 16 Error Messages Alas 10 17 Display Control Summary 10 18 Remote Modea r ee e e de e v ERE CE SANA EU d 10 18 Stand Alone Mode 10 19 Running a Local Profile 10 19 Review Screen sov vs eek korr B VERE CLE UE 10 19 Information Fields 10 19 User Controls ibas MuR DA t ares DM apasaq 10 20 EE 10 23 Plot Color Selections 10 23 Information Field Set Up EE 10 24 Default Directories 10 24 User Name and Password Maintenance 10 25 A on diese ge Hl eee elaine 4 10 26 Sharing Host PC Resources 10 26 vi 7007452 CryoMed Freezer Thermo Scientific Thermo Scientific Section 1 Introduction Thermal printer Control panel Figure 1 1 Non IVF Controlled Rate Freezer hermocouple connection NN Figur
115. sting of parameters with their defaults Definitions cont Size L M S default is M Cycle Time adjusted over a range of 500 to 2000mS default is 1000mS P Proportional gain adjusted over a range of 0 0000 to 0 9999 default 0 25 I Integral gain adjusted over a range of 0 000 to 0 999 default 0 075 D Differential gain adjusted over a range of 0 00 to 9 99 default 0 20 D C Discontinuous or Continuous operation of the controller when the probe temperature is the same as the set temperature default C PR Cool Cool solenoid precision minimum on time adjusted over a range of 0 01 to 0 99 default 0 01 or 50mS minimum on time CP Cool differential temperature adjusted over a range of 1 C to 99 C default 4 C CM Cool multiplier determines the cooling proportional and differential gain constants relative to the constants entered for P and D i e P cool P CM with a range of CM from 0 1 to 9 9 default 1 0 Use Factory Defaults This menu item restores the factory set parameters for the following items Access Code 0 0 0 0 disabled Probe Calibration offset and scaler 0 Simulation Off Cool 1S 10S 90C m Heat 1S 45S 9C m Thermal Controller Size M 1000mS P 0 25 I 0 075 D 0 2 PR 0 01 CP 4 CM 1 0 C Tracking Alarms 5C 1m Note User Profiles are not affected A Thermo Scientific 7007452 CryoMed Freezer 3 7 Section 3 Configuration Calibration M
116. swords may be set up to protect all entries into the P y P to p Maintenance Edit Run Review and Options menus Save before leaving the Users screen 1 Typea name into the User Name window 2 Type the desired password in the Password window 3 Confirm the password in the Confirm Password window 4 Click Add button to add the user name and password to the list 5 Repeat for any additional user names 6 To change a password select the user name from the pull down list or type the name in User Name window enter the new password and click the Modify button 7 To delete a user name select the user name form the pull down list or type name in User Name window and click the Delete button 8 Click Save to keep changes 9 Click Restore to return to previously saved changes 10 Click Exit to exit Options screen If no user names are defined all menus can be accessed without password protection Anonymous is listed as the user name if no users are set up If password protection is no longer desired simply delete all user names CHE Configuration Options ES Plot Colors Information Fields Default Directories Users Save Add a new user by entering the user name and passwords in the boxes below then click the Add button Restore Madify the password for an existing user by entering the user name and the new password in the boxes below then click the Add button Delete a user by entering the user name below then click the De
117. t be on the same line as the selector Press the Run key If the chosen profile begins with a Wait the Run key must be pressed again to continue after the appropriate temperature is reached The mode indicator is lit showing the status of the profile 7007452 CryoMed Freezer 2 5 Section 2 Set Up and Operation 2 6 Initiate a Profile continued Stop a Profile Run 7007452 CryoMed Freezer Note Any profile can be started at the second or any subsequent step by entering View Mode press Enter scrolling pressing the 5 6 keys to the desired profile step and pressing the Run key If Access Code protection has been enabled the unit will prompt for the access code before entering Run Mode at the selected step A Note The user can manually move to a different step in a running profile by scrolling 5 6 to the desired profile step and pressing the Run key the move occurs when the Run key is released If the user does not press Run within 30 seconds or Back to abort the move the control will automatically exit the the move mode and continue profile execution without interruption A If Access Code protection has been enabled see Configuration Calibration section the unit will prompt for the access code before moving to the selected profile step A profile run can be discontinued by pressing the Back key If Access Code protection has been enabled the unit will prompt for the access code before terminating the profile
118. t is running a profile initiated from the freezer keypad In this case the PC cannot take control Select the Terminate Session button to close the Run window 7007452 CryoMed Freezer 10 17 Section 10 PC Interface Software 10 18 Error Messages continued Display Control Summary Remote Mode 7007452 CryoMed Freezer Cannot stop freezer in Stand Slone mode Stop capturing data Continue capturing data k Cannot Stop Freezer in Stand alone Mode This message displays if the Stop button is clicked while the freezer is running a Remote profile in Stand alone mode Clicking the Stop button at this time stops only the data capture Choose Continue capturing data or Stop capturing data No other commands can be sent from the PC Three modes are available when using a Controlled Rate Freezer and PC configuration Remote Mode Stand Alone Mode and Running a Local Profile The PC can initiate all actions at the freezer such as Run Stop Warm Manual Over ride Alarm Silence and Alarm Clear The PC displays the current freezer operation status Idle Run Wait End Warm and the chamber and sample temperatures Time and temperature details are captured every 6 seconds The CRF keypad is disabled except for the Silence key which can be used to silence the audible alarm and clear alarm messages at the freezer Alarm messages on the PC must be cleared at the PC as an added
119. tarting point Thermo Scientific Access Code Security Initiate a Profile Thermo Scientific Section 2 Set Up and Operation The controller will provide security for the following menus and functions if the Access Code is enabled by prompting for a 4 digit numeric access code Configuration Calibration Menu Profile Edit Menu Manual Override during a run Start of a run on second or subsequent step in a profile Manual change in Run profile execution to a prior or following step Premature termination of a profile run If access code protection is enabled see the Configuration Calibration section access to the above functions is gained by entering the access code as follows Use the Enter and the Back key to select the desired digit Use the up or down arrow keys to change the digit When finished use Enter to confirm entry of the code and to move into the selected protected function or menu The access code when protection is disabled is 0 0 0 0 The access code can be enabled changed or disabled by using Change Access Code see Configuration Calibration section Load the product into the freezing chamber On IVF models make sure all unused sample access ports are capped If the profile list is not on the display press Back until the list appears Locate the item selector on the left of the top line of the menu Move the required profile to this line by using the 5 6 keys The chosen profile mus
120. the illustration following The starting point of each Step is indicated on the graph line by a small square The graph can be moved horizontally and vertically by using the scroll buttons Scale adjustments can be made using the time and temperature pull down menus i il n ee Temperature Division derece Chamber 2 7 ee aeu COOL BHEB casar i 20 U U 1 1 6 8 10 1 minutes D D T EZ 12 43 34 15 16 1 19 19 Figure 10 8 Edit Window Graph Error Messages If selected step parameters are out of range an error message will appear when the Enter or Replace button is pressed Press OK and correct the parameters When saving a profile which has the same name as one already existing a file overwrite error message will appear Choose YES to overwrite replace or NO to enter a new file name Thermo Scientific 7007452 CryoMed Freezer 10 9 Section 10 PC Interface Software Run Screen User Control Definitions 10 10 7007452 CryoMed Freezer Data Tag is mandatory information entered by the user which is used as a prefix for the file name in which the run data is stored Note If illegal characters U V lt gt all lower case word run are used in the data tag the program runs normally but no data file is created Date Time is information taken from the PC system clock and stored with the run data User Name is supplied by the security module of the software Anonymous is used
121. tion of the serial communication interruption Experiment with test runs or ifin doubt don t print during a critical run Using other applications on the same PC with a Run in progress may cause the loss of Run data points Serial port communication may stop for a short time if PC resources are consumed by other applications Chart lines continue across the communication gaps Slow ramps or holds will lose very little data However steep ramps may be more critical Experiment with test runs or ifin doubt don t run other applications during a critical profile run If there is a loss of communication between the PC and the freezer for more than 20 seconds the freezer may go into Stand Alone mode A slow PC lt 166MHz with limited resources lt 32MB RAM may stop serial communication for more than 20 sec if multi tasking PC control would be disabled for the duration of the current Run Run data is lost during the time of the communication interruption Experiment with test runs or ifin doubt don t run other applications during a critical profile run A Windows crash may stop serial communication for more than 20 seconds The freezer will go into Stand Alone mode and PC control will be disabled for the duration of the current Run Run data will be lost during the time of the communication interruption Experiment with test runs or ifin doubt don t run other applications during a critical profile run Thermo Scientific W
122. uct conforms to the following Harmonized International and National Standards EMC LVD EN 61326 1 1997 EN 61010 1 1993 EN 50081 1 92 Amendments 1 and 2 EN 50082 1 97 CSA C22 2 No 1010 1 Richard L Miller COE Regulatory Compliance Manager ThermoFisher SCIENTIFIC 18 June 2008 7007452 CryoMed Freezer AA 3 Declaration of onturmito Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Units Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 U S A Controlled Rate Freezer 7474 2001 Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC LVD 89 336 EEC 73 23 EEC This product conforms to the following Harmonized international and National Standards EMC EN 61326 1 1997 EN 50081 1 92 EN 50082 1 97 LVD EN 61010 1 1993 Amendments 1 and 2 CSA C222 No 1010 1 Declaration of Conformity Manufacturer s Name Manufacturer s Address Product Description Product Designations Year of Initial Marking CE Affected Units Thermo Fisher Scientific Asheville LLC 401 Millcreex Road Marietta Ohio 45750 U S A IVF Controlled Rate Freezer 7475 2001 Release 2 Release Level REL shown on Serial Tag This product conforms to the following European Union Directive s EMC MDD 89 3
123. window opens for the option of adding for example notes pertaining to the Run or samples Click Continue to begin capturing temperature data from the Controlled Rate Freezer Run data will not be captured until the comment window is closed Stop the freezer after the completion of the run and removal of the samples The freezer holds the last chamber temperature indefinitely at the End step The Run may be stopped by clicking on the Stop button or pressing the Back key on the freezer keypad Enter any additional comments when the text entry window opens again This window appears regardless of the Run stop method 7007452 CryoMed Freezer 10 15 Section 10 PC Interface Software Typical Run Procedure cont Power Failure Recovery 10 16 7007452 CryoMed Freezer 11 12 Initiate Warm Mode The Warm button appears after the Run stops and the comment window is closed Click the Warm button or press the Warm key on the freezer keypad The PC monitors the freezer s warming status Terminate Warm Mode and close the Run screen with Exit button Warm Mode can be terminated by clicking the Warm button or by pressing the Back key on the freezer keypad The PC monitors the Warm status regardless of the method of termination The Run screen may be closed without terminating the Warm mode by clicking the Exit button The freezer continues to warm until it is either stopped by the user at the freezer keypad or automatically 3
124. ys Op en depending on what the unit is doing at the time or which freezing profile has been selected 1 Idle Mode If the probe fails or is removed by the operator during sample loading while unit is in Idle Mode Alarm LED lights no audible alarm LCD Display shows S sensor fail on the fourth line Sample numeric display shows ILL The operator may start a run that does not reference the Sample temperature if the Sample probe is removed or open and the Alarm LED and the fourth line LCD message are cleared using the Silence key The run will proceed normally If the operator attempts to start a run using the Sample temperature for a trip point Audible alarm sounds Run start prevented LCD Display shows S sensor fail on the fourth line alternated with S sensor req The Alarm LED and display messages remain active until the probe condition is resolved 2 Run Mode If the Sample probe fails during Run Mode and the current profile uses the Sample temperature as a trip point Alarm LED lights Audible alarm sounds continuously LCD Display shows S sensor fail on the fourth line alternated with S sensor req Sample numeric display shows ILL Immediately switches to Idle Mode Disables all control functions Pressing the Silence key will mute the audible alarm however the Alarm LED and display message will remain active until the probe condition is
125. zer Thermo Scientific Section 2 Set Up and Operation Holder Adjustment The vial and straw holders may be adjusted to the size needed Refer to the illustrations following 1 Loosen the small hex socket set screws in the top of each holder 2 Rotate each of the long adjustment rods equally to the right to make them longer widen the opening or to the left to make them shorter narrow the opening Note the threads at the bottom of the adjustment rods 3 Tighten the small hex socket set screws when the adjustment is complete just until the screws lightly touch the rods to avoid damaging the rod threads Hex socket Hex socket set screws set screws Adjustment rods Figure 2 7 Vial Holder Adjustment Adjustment rods Figure 2 8 Straw Holder Thermo Scientific 7007452 CryoMed Freezer 2 21 Menu Structure Menu Entry Definitions Thermo Scientific Section 3 Configuration Calibration The Configuration Calibration Menu is selected by pressing the Enter key with the Configure Calibrate line selected The software will prompt for the Access Code if enabled prior to entering the Configuration Calibration Menu The following is the menu structure Configure Calibrate Change Access Code Chart Recorder Printer Probe Calibration Service Manual Control Print Error Logs Reset Error Logs Simulation Software Checksum Test Ram Thermal Controller Use Factory Defaults Time and Date Tracking Alarms 1 Configure

CryoMed Controlled-Rate Freezers - User Manual

Contents

Download Pdf Manuals

Related Search

Related Contents