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BioPAT ® ViaMass

1. Facer CH 18 12 2005 113532 TTT debe AES 13 The Probe Status tab is very similar to the Futura Status but looks at information taken from the probe instead The only extra information shown is the number of probe leak detections and calibration details 38 Using Futura Tool Each probe has an internal moisture sensor If this senses any moisture inside the probe casing the Number of Probe Leakage Detections value will increment If it shows any value other than 0 the probe s integrity may be compromised and should be checked Contact your distributor or Sartorius Stedim Biotech for advice 9 3 Operating Modes Tab The Operating Modes Tab is the main area for configuring your BioPAT ViaMass It is split into four sections Hardware Settings Measurement Settings Clean and Zero Functions Futura Connect Hardware Mode only and Live Readings Talasa LI KI I pr Lija Ji De pura en arpata Dic MASOGOE ergo Parthus Paie Haha Opee ede Gik aie Adare Degrees Phan pein Se Hatderare Here Futura Connect dde T anjar Bete bert rar Monte sete ieties isai Hoe Pan fumas 9 3 1 Hardware Settings The Hardware Settings area displays the current hardware mode of the connected BioPAT ViaMass and any associated settings Hardware modes available are USB Only Mode Used when connected to a BioPAT ViaMass Hub when not using the Modbus interface or Current Loop outputs
2. 10 Troubleshooting This chapter covers some technical difficulties that you may encounter while using your BioPAT ViaMass system 10 1 Noisy or Unexpected Readings If you are experiencing unusual readings you should try the following Check probe placement Readings can be affected by obstructions in the probe s field of view Check the Measurement Options One of several Measurement Modes may be selected depending on the application and cell type being measured Ensure you use the correct Measurement Mode or settings if using Custom Mode for your application See Chapter 8 Measurement Options page 28 for further details Check that the BioPAT ViaMass is correctly earthed See section 7 5 Earthing the BioPAT ViaMass page 26 for further details Check probe calibration settings It is possible to create custom probe calibrations to cancel the effect of obstructions in the probe s field of view Check that the correct calibration is being applied for the equipment being used 10 2 Noisy Capacitance Readings A high level of noise on the Capacitance measurement in particular is typical of a poorly earthed BioPAT ViaMass Check that the BioPAT ViaMass casing is properly and securely earthed using the supplied earth strap see section 7 5 Earthing the BioPAT ViaMass page 26 and that the BioPAT ViaMass electronics and probe are located as far as possible from any large electrical machinery
3. Note that in measuring or calculating the volume fraction the relevant parameter is that enclosed by the cytoplasmic membrane The radius of this is typically 1 1 to 1 5 times less than the microscopically observed cell radius The BioPAT ViaMass therefore provides the means to Measure C at chosen frequencies in the range 0 05 20 MHz Correct these for the background prior to inoculation or during the fermentation Output the data in a user defined unit like mg ml after calibration against the method chosen by the user e g cell dry weight Detailed Theory of Operation 51 57 Detailed Theory of Operation 11 3 Conductivity The energy in the exciting electrical field can only be stored as C or dissipated as G For fundamental reasons therefore any increase in C must be accompanied by a decrease in G The measuring principle exploited in the BioPAT ViaMass also provides the user with a measurement of the electrical conductance of the cell suspension and will be frequency dependent increasing with frequency Because the cells take up a certain volume fraction the low frequency conductivity o L of the suspension will be lower than that of the suspending medium o by an amount that depends upon the cellular volume fraction P according to f jj 1P 8 o L O o 1 P 2 The conductivity of a fermenter medium may not be constant as it depends upon the uptake production and excretion of charged metabol
4. 4 2 3 Connecting Probes and LADIES Asian kana mter E nth 13 4 2 4 Connecting the Single Use Sensor Patch lt lt 13 4 2 5 Power BioPAT ViaMass Connect Hub 1 08 14 4 2 6 Probe BioPAT ViaMass Electronics BioPAT ViaMass once La ir E T 14 4 2 7 Connecting Your BioPAT ViaMass to a PC L 14 4 2 8 Assembly Connection of BioPAT ViaMass Elektronics to a BIOSTAT RV tc ordeo ais Uns edad Gs adit oda aru erae a d 15 4 2 9 First start up with control unit 16 B SSVSIEIID E DOOR sirenai di d ENA dam Cra ada i i dim bei ERE 17 5 1 Using PUUUIA OO osaiecssenr ana aa ei god phar EUER PRU RE i ERE dau ed 17 5 2 System Check with DCU Integration eater cecus tira ee i Dona 18 5 3 Displaying System Information on the BioPAT ViaMass 20 6 Software Installation aaa akiai kaka aaa 21 6 1 Futura fool a kekes ss ais i i d us p a i a i ki 21 6 2 Minimum System Requirements tas sios i Re i ERI Pim 21 63 clit ias S ii ar e a ri a i a a e a 21 6 3 1 Microsoft Net Framework 4 0 aaa 22 63 2 FIDI Drivers 0 0 ccc enn nnn ence eens 27 Contents 3 Contents 7 BioPAT ViaMass System Components aaa aaa 24 7 1 BioPAT ViaMass Care and Maintenance aseo pai et ond eet a is 24 L2 How to Store Your BIoPAT ViaMass acd ba et oi E ei a ii 25 7 3 How to Clean Your BioPAT ViaMass J aaa aaa 25 TA PIMA DOLUS sias Ber vases eg eai x Duas aeq use RO vus Uds i
5. 10 3 Lid Lock Warning E10 Error The BioPAT ViaMass features an anti tamper feature that will disable the BioPAT ViaMass if the lid is removed Do not open or disassemble the BioPAT ViaMass Doing so will invalidate the warranty There is a risk of electric shock and there are no user serviceable parts inside If this error is observed under normal conditions please upgrade to the latest BioPAT ViaMass firmware see section 7 4 Firmware Upgrades page 25 If the problem persists please contact Sartorius Stedim Biotech or your authorized distribu tor for assistance 10 4 BioPAT ViaMass Busy E02 Error The BioPAT ViaMass does many tasks internally in order to operate correctly Occasionally a task is being completed that means a measurement cannot be completed at this instant Under these circumstances the BioPAT ViaMass will report that it is busy The system will usually just deal with this situation and you will not be aware of it It is possible to observe this error under normal conditions if it does not clear within a few seconds please upgrade to the latest BioPAT ViaMass firmware see section 7 4 Firmware Upgrades page 25 If the problem persists please contact Sartorius Stedim Biotech or your authorized distributor for assistance Troubleshooting 47 48 Troubleshooting 10 5 BioPAT ViaMass Not Recognized by Computer If you are having difficulty connecting your BioPAT ViaMass to Futura
6. Comic tity mSjcm Filter settings 120 As seen in the graph above the Capacitance reading fluctuates by up to 6 pF cm when the earth strap is removed in an electrically noisy environment even though the system has high filtering on it The amount of fluctuation will vary depending on the environment IMPORTANT 7 6 BioPAT ViaMass Cables Care must be taken when handling the BioPAT ViaMass units The 1m link cable between the two enclosures should not be bent kinked coiled tightly or repeat edly flexed as it may weaken the cable and cause it to fail If placed into a rock ing system it is recommended that both enclosures are placed on the platform so that a replaceable extension cable takes any repetitive flexing that may occur Please follow good layout practice and avoid tight bends in the cable especially near the BioPAT ViaMass enclosure and connectors During routine disconnection of the BioPAT ViaMass from the probe ensure that the BioPAT ViaMass is stored in such a way that the cables are not stretched or folded in any way If required create a generous coil for the cabling The BioPAT ViaMass cables must NEVER be cut The connectors must not be removed The cables should be protected from mechanical damage The connectors should be protected from excessive water and corrosives Sartorius cables should never be excessively bent or kinked As a precaution the BioPAT ViaMass cabling should avoid clos
7. Dual Frequency High Frenzy Pal Ceeroction Rocher Filter Custom Mode Only use the Custom Mode if you have received training from Sartorius or under the supervision of a Sartorius expert Wrong configuration of Custom Mode parameters can lead to erroneous or imprecise measurements Under normal circumstances one of the three modes is used for cultivation or fermentation The Custom Mode can by switching back to one of the factory set modes in case one of these has been activated inadvertently The submenu Custom Mode displays the configurations for the user defined mode To use the BioPAT ViaMass in a BIOSTAT RM you must activate the rocker filter gt Touch the button Rocker Filter The field now displays the value ON gt Confirm these changes with ok 8 2 Zeroing The Zero function simply tares the capacitance output of the instrument For example if you have a signal that is 7 pF cm and during the course of the experiment it rises to 12 pF cm then the total change is 5 pF cm This 5 pF cm change equates to the viable biomass measurement The Zero function allows you to remove the 7 pF cm offset So if you zero the instrument in the initial 7 pF cm solution the output will rise from 0 pF cm to 5 pF cm during the course of the experiment The BioPAT ViaMass has a built in Zero function which can be triggered over Modbus or in the Futu
8. Futura Connect Mode Used when connecting to a Futura Connect when using the Modbus interface and or Current Loop outputs Rocker Bag Mode Used when connecting a BioPAT ViaMass and using a rocker bag To change hardware modes click on the Edit Hardware Mode button in the Hard ware Settings pane This will bring up the Hardware Modes screen The contents of this screen will vary depending on the currently selected Hardware Mode and is described in the following sections 9 3 1 1 USB Only Mode If selecting this mode no additional settings are required Ergani Fer Sara Chance beter Ey nci rana sinum o Far cote Purus dan thee moe a pet ha cau Frc ana Cee gt Comes welt WTR F Bod r uten Los bes run Click the Save Changes button to confirm your selection Using Futura Tool 39 40 Using Futura Tool Your selection will be displayed in the Operating Modes tab in the main Futura Tool The Measurements Settings will no longer be visible as the only way to obtain readings from the BioPAT ViaMass is now through Futura Tool software which feature software based Measurement Settings such Quick Readings tab in Futura Tool Eni mod nr Rl ars aces dmc Cin See asm a VT S P hipa ad LENA 9 3 1 2 Futura Connect Mode If selecting this mode you may need to enter Current Loop and or Modbus settings depending on your application I r45 iasi ee MESRNRMEN praemio E a Dmi m B To
9. and direct warning against hazards all text statements to be particularly noted in these operating instructions will be marked as follows This symbol denotes a possible danger with risk that moderate or minor injury A CAUTION may result if it is not avoided This symbol denotes a danger with low risk that could result in property damage IMPORTANT if the risk is not avoided his symbol isan indication of a function or setting on the device that caution should be exercised while working identifies useful information About this Document 5 About this Document IMPORTANT The following presentations will also be used Texts that follow this mark are lists P Texts that follow this mark describe activities that must be carried out in the Specified order gt gt Texts that follow this mark describe the result of an action Texts in quotes are references to other chapters or sections 1 5 Structure of the Manual The aim of this manual is to provide both a practical guide to setting up your BioPAT ViaMass system and a comprehensive reference for each element of the BioPAT ViaMass system If you are familiar with our technology and general software installation we recommend that you read the rest of this introductory section the 4 Quick Setup Guide Seite 12 and System Check 5 System Check Seite 17 as a minimum referring to later chapters where necessary Other chapters
10. in Hz has the appearance of an inverted sigmoid with a strongly frequency dependent region separating two plateau regions The following figure is typical for microbial cell suspensions gt 2 1 Fun P ae 6 Log Frequency Hz Graph Showing the Beta Dispersion Typical of Microbial Cell Suspensions Measurement of e or C in or near the lower frequency plateau region therefore provides a means of estimating the biomass present The frequency at which this effect the B dispersion is half completed is known as the characteristic frequency and 4 f 1 211 Typical values of many fermentations have characteristic frequencies near 1 MHz for the B dispersion as may be discerned from equations 3 and 4 or from the monograph published in J Bioelectricity 5 139 144 1985 Some cells especially Gram positive bacteria possess an additional dielectric dispersion the so called a dispersion which occurs due to ionic processes in the cell envelope However this dispersion is observed at frequencies lower than that of the D dispersion Whilst this effect is also a function of the biomass content present the nature of the cell envelope can change more than the nature of the cytoplasmic membrane as a function of physiological state independently of the biomass content The frequencies of operation of the BioPAT ViaMass are therefore designed to exploit the p dispersion alone The relative density mass per
11. include more detailed descriptions of the individual components in your BioPAT ViaMass system and the various measurement options available to you Comprehensive guides for setting up your BioPAT ViaMass as a standalone system or as part of a larger control or measu rement system are also included Technical Specifications Software Licence Agree ments and EMC Regulatory Information sections can all be found towards the end of this manual Please read this manual fully and carefully before starting to use this equipment Failure to comply with the appropriate safety guidelines may cause severe injury or property damage This symbol on BioPAT ViaMass equipment warns the user to consult the appropriate parts of the User Manual before making any connections Wrong connections voltages or currents outside the specified operating ranges could cause danger or permanent damage to the equipment IMPORTANT 2 Safety Instructions 2 1 General Information Sartorius Stedim Biotech GmbH supplies the BioPAT ViaMass after careful testing in ready for operation condition This manual contains instructions for the safe opera tion of the BioPAT ViaMass You may only operate the device under conditions that comply with the specificationsstated in this manual and the information on the manufacturer s ID label 2 2 Authorized Personnel The following persons may carry out the actions described herein Operations Persons Montage und Ans
12. the Screen to connect to any single BioPAT ViaMass that is attached to your computer The channels on your BioPAT ViaMass Connect Hub will appear as COM ports in Microsoft Windows 9 1 1 I know which COM Port my BioPAT ViaMass is connected to If you already know which COM Port you have connected your BioPAT ViaMass to you can save time by selecting the port in the Quick COM Port Selection list If you connect your BioPAT ViaMass and or hub to your computer after you have launched this screen click Refresh Port List for new COM Ports to be listed in the Quick COM Port Selection list After a selection is made the Connect button becomes active click on it to connect to the BioPAT ViaMass 9 1 2 I am not sure which COM Port my BioPAT ViaMass is connected to The search facility can be used to find all BioPAT ViaMass connected to the PC but not in use by other applications The Connection screen above shows an example of a completed search on a PC that had two BioPAT ViaMass devices connected through a BioPAT ViaMass Connect Hub The search facility s speed varies depending on the number of COM Ports available on the PC A computer with more COM Ports that are connected but not in active use will take longer to complete the search Click on the BioPAT ViaMass you want to use then click on the Connect button Futura Tool will only work with one BioPAT ViaMass at a time After successful connection the
13. which by experiment is found to have the value 8 854 x 107 F cm The units of o are therefore mS cm whilst is dimensionless The value of e for water at 298 Kelvin is 78 4 so that it may be calculated from equation 2 that a cell of unit dimensions therefore K 1 cm has a capacitance of some 6 94 pF Since the electrode geometry does not change during a fermentation it is suitable for the purposes of estimating cellular biomass with the BioPAT ViaMass to talk in terms of C and G alone However for purposes of generality what follows will be described mainly in terms of o and e Of course the electrodes used with your Detailed Theory of Operation 49 50 Detailed Theory of Operation BioPAT ViaMass are not plane parallel Nonetheless they possess an effective cell constant or probe constant that may be used to relate the measured C and G to the actual o and e In all BioPAT ViaMass products the probe type is recognized automatically and software ensures that the correct probe constant is applied and readings are always converted to pF cm and mS cm no matter what probe type is plugged in 11 2 Beta Dispersion In contrast to those of ionic solutions the passive electrical properties dielectric properties of cell suspensions are strongly frequency dependent a phenomenon known as dielectric dispersion All biological cells possess what is known as a D dispersion of a magnitude which is both pronounced
14. AT ViaMass aaa aaa aka aaa 37 9 1 1 I know which COM Port my BioPAT ViaMass is connected to 37 9 1 2 lI am not sure which COM Port my BioPAT ViaMass SEO COOL pass ss asas saras a Lai ka a S a E 37 SL Fura TOOLIEHIUL cou e Peas Sa a o S I o As 38 914 fura ta GUS TO paie ai ai E i aa E ms 38 92 Proge status A cT 38 sm ESdc Mui eg ocT EM 39 931 Ha tdware TMC a a es usa e ss a S 39 9 3 1 1 USB Only dVIOU E Loader ia is i i oer SOR oa ames 39 9 3 1 2 THES Connect M d erosut rs i vu i voe Parier d 40 9 3 13 Rocker Bag o P asas e se ass i aa 42 2 Measurement SC LIMOS ses as i utut os disse Tie v ed Rea 42 gus Clean and Zero FUINCHIONS sitas ntis d RI asa a dor eds 43 OOF Live Readings ae a as ri sa a a i RUVERRE MEUM VP EE 44 9 4 Ouick Readings Olekas ask sns d ka Pako a i aiiai e Enero SRE tance 44 9 5 F t ra Oruro ies sta esu i ss a o a caves weds 46 10 Troubleshooting ttutuuisssssssssssssssss Ie 47 10 1 Noisy or Unexpected Readings Larsas i sia e RO PR e TN Equites Uus 47 10 2 Noisy Capacitance ReaulftS utente dm ERR ect oe RR ERE Ra 47 103 Lid LOCK Warning ETO EION 47 10 4 BioPAT ViaMass Busy E02 Error as 47 10 5 BioPAT ViaMass Not Recognized by Computer 48 11 Detailed Theory of Operation aaa aaa 49 11 1 The Electrical Properties OF Living Systems Lekas i i et a eta 49 11 2 Beta DISpersiON st cosse ss ss nis e
15. Biotech GmbH Goettingen Germany Printed in the EU on paper bleached without chlorine W Publication No SBI6015 e141001
16. T MGLE Ww ELO ee ick CONDE RETI Fir nore proram nad he Curs Colecam rome Tri Mite rout HET Fearanwects d Setup Installation Is Complete H1 Erie Sind niuis re ll Software Installation 23 24 IMPORTANT BioPAT ViaMass System Components 7 BIoPAT ViaMass System Components The BioPAT ViaMass is designed for use with Sartorius Stedim Biotech s range of disposable probes that are suited to be used with Flexsafe RM or Flexsafe STR BioPAT ViaMass comprises of a primary RFI shielded copolymer enclosure for the main electronics and a second copolymer enclosure for the probe connector on a 1 m umbilical cable both enclosures being RFI shielded and rated to IP41 The front end probe connector enclosure is a lightweight design to minimize stress on the probe and bag Note that as the BioPAT ViaMass is designed for use with disposable probes BioPAT ViaMass signal simulators must be used for system checks as the standard signal simulators will not fit See section 5 System Check page 17 for further details on using signal simulators There is no Clean Pulse function present on the BioPAT ViaMass single use system Care must be taken when handling the BioPAT ViaMass The 1 m link cable between the two enclosures should not be bent kinked coiled tightly or repeatedly flexed as it may weaken the cable and cause it to fail Nor should any other BioPAT ViaMass cables be excessively ben
17. TES 25 TAI WOPOFBOHISITOS Kasis s k Tor ga sai res is dor S ret aa L SS RUINA 25 7 4 2 Firmware Upgrade INSWUCTIONS scacsn cunt senis as Lk sak ra ete ns ZB 75 Earthing the BIoPAT VIalVIass s eoe enero npR rhe gras RE REF A dee seekers 26 zB dBiepPAT ViaMass dO CS accru eps S CHOR AT EEUU p TENER 27 8 Measurement Options ssssssssslsss eee 28 8 1 Measurement Modes Description sai ed coe ir reb ed tete ne etas 28 8 1 1 Measurement Modes Available 28 81214 Cell Culture MOJE vs aa aaa Ea ren a SEE FRU ERAS 28 8 1 1 2 Bacterial Mode i 2055 2icbncddacnmigadsmaksddaeencaadawaenss 28 SLLLS Microvdl ModE oce uarie Eug aetas meti ntes utem rbd 29 8 1 1 4 Custom Mode aaa 29 8 1 1 5 Signal Simulator Mode Ak ss ais Ss sa sr tne oen a 29 8 1 2 Selecting a Measurement Mode 29 SOME DLA OO arka e a a k r i a a 30 8 1 2 2 Selecting the Measurement Mode via DCU 31 MEME IIO UU m be eee pene eine 34 8 2 1 Zeroing Using Futura Tool aka 33 8 2 2 Zeroing Readings on the DC U ecu cedent ett nor nets 34 8 3 Converting the Capacitance Value to Cells per Milliliter C ml 34 8 4 Settings for Alarm Limits aeuo eorr eae etre rae Dn teo a mes 35 8 5 Polarization Correction isses 36 BiG FCI C MER TEE TT TTL QT TROU 36 8 6 1 Rocking Motion Monitoring 36 9 Using Futura Tool sssssessess e tte e nets 37 9 1 Connecting to BioP
18. ach BioPAT ViaMass must use a different Modbus address If address conflicts occur the Futura Connect status lights of the conflicting channels will turn red Other Settings Biomass Factor CPM per pF em oO The Biomass Factor is a scaling factor that is applied to the capacitance reading to calculate a viable biomass figure This viable biomass reading is calculated within the BioPAT ViaMass and is available over Modbus and Futura Tool software Using Futura Tool 41 42 Using Futura Tool Click the Save Changes button to confirm your selection A summary of your settings will be displayed in the Operating Modes tab in the main Futura Tool window 9 3 1 3 Rocker Bag Mode The settings for this mode are the same as those available in Futura Connect Mode Current Loop Advanced Current Loop Modbus and Biomass Factor settings Please refer to the Futura Connect Mode section 9 3 1 2 Futura Connect Mode page 40 Tawmularl Peri Tarot ekiai Damon Ej jud Pepe justus Vo Fate Giraud Pula cometa tegt pe ome ce a J me When in Rocker Bag Mode the BioPAT ViaMass attempts to filter out the variation in readings caused by the probe electrodes becoming uncovered due to the bag rock ing back and forth Only use this filter if you are measuring in a situation where the liquid level above the electrodes varies and is 3 cm or less 9 3 2 Measurement Settings If available in the selected hardware mo
19. and dependent upon the amount of cells present It is the measurement of the magnitude of this D dispersion which enables the BioPAT ViaMass to distinguish cells from other particulate matter aqueous solutions and gas bubbles All biological cells consist in electrical terms of a poorly conducting lipid membrane separating a conducting cytoplasmic volume from the conducting extracellular bulk volume including any cell wall if present The electrical capacitance of this lipid membrane is an order of 1 to 2 uF cm dependent on the type of cell considered As the frequency of measurement is lowered the electrical field is more efficient at charging this membrane capacitance This results in a measured capacitance between the electrodes increasing greatly as the frequency of measurement is lowered Because the current carriers in the cytoplasm and extracellular fluid are ionic and must reach the cell membrane before they can charge it up the time taken to do this is called the time constant v and depends on the internal cytoplasmic 0 and external o conductivities the mean cell radius r taken across the cytoplasmic membrane excluding the cell wall and the magnitude of the specific membrane capacitance per unit area C B rr gt Or 20 o After correction for the frequency independent background permittivity or capacitance of the suspending medium a graph of e vs log f the logarithm of the measuring frequency
20. and is also familiar with the installation of software within the Microsoft windows framework Details on the workings of BioPAT ViaMass technology and the installation of soft ware are expanded upon in following chapters The following is a description of a typical system and allows the user to unpack recognize each element install the software onto a PC connect it together and check that it functions properly 4 1 Typical System Checklist A typical BioPAT ViaMass system consists of the following SU Probe welded into a Flexsafe RM or Flexsafe STR bag BioPAT ViaMass electronics Two Signal Simulators 0 pF cm 0 mS cm and 100 pF cm 40 mS cm USB flash drive containing Futura Tool BioPAT ViaMass System Manual this document Accessory for service and retrofit versions only BioPAT ViaMass Connect Hub 1 External Power Supply Unit Mains power cable Set of green phoenix connectors for Current Loop and Modbus USB cable 4 2 Getting Started 4 2 1 Unpacking It is recommended that you unpack all the items and lay them out in a clear space Check all items for signs of damage If you suspect an item to be damaged or if anything appears to be missing contact the shipper and Sartorius Stedim Biotech or your local distributor within three days of receipt of the shipment The packaging in which the items were dispatched is specially designed to protect them during transport We re
21. ation entry on 64 bit Windows systems If it becomes necessary to uninstall the drivers find the application entry below in Control Panel gt Add or Remove Programs or the equivalent and uninstall that application See Windows help for more information on removing applications IMPORTANT FIDI virtual COM port drivers are required in order for Futura Tool to recognize and be able to connect to a BioPAT ViaMass through USB The drivers supplied as part of the installation software have been tested to ensure that they work with BioPAT ViaMass hardware Newer drivers may be available in future from Windows Update but there is no guarantee that the newer drivers will be as stable and work as well as the ones supplied The Setup Wizard will install Futura Tool on your PC once you have clicked Next accepted the terms and conditions and checked you have the right folder for installation again This will take several minutes click on Finish to complete the Futura Tool Setup Wizard You may be prompted to restart your computer You must unplug all BioPAT ViaMass Connect Hubs from the computer before installing Futura Tool IJ sLM STI E Salig ina I lramsework 4 hetup Fu S aae PEES Fx Pie aoe En licence berger He ori HE Placa puel ahs fhe IET eee ic berg insule NET m MICROSOFT SOFTWARE i Irina ad ated peace Hh enne tcm a dl Tiimasdecard dant ueeea LI Deri ime cae Dali 2 miro pesa d mri UM Gg NT
22. ays disconnect the sensor connector of the BioPAT ViaMass from the bag before it will be removed from the bag holder Ignoring this procedure may result in the destruction of the cables or the bag 4 2 5 Power BioPAT ViaMass Connect Hub 1 If you are using a BioPAT ViaMass Connect Hub 1 plug in the mains power cable to the external power supply unit and then attach the power supply unit to the BioPAT ViaMass Connect Hub 1 Tighten the locking ring on the power supply plug by hand to ensure that it does not come dislodged from the unit when in use Ensure that the mains power supply is switched on The power indicator light on the front of your BioPAT ViaMass Connect Hub 1 will flash when the unit is initially switched on This should settle to a steady green light to indicate that the unit is powered and operating correctly 4 2 6 Probe BioPAT ViaMass Electronics BioPAT ViaMass Connect Hub 1 Connect a probe or Signal Simulator to your BioPAT ViaMass and connect the BioPAT ViaMass electronics to a socket on the front of your BioPAT ViaMass Connect Hub 1 ensuring that the colored dots on all plugs and sockets are aligned The status indicator light will display a steady green light once a connection between the BioPAT ViaMass and BioPAT ViaMass Connect Hub 1 has been success fully established Please note that it may take several seconds for a connection to be establishhed If the light does not come on or i
23. bmenu ViaMass CAP The submenu Info CAP is displayed Field Zero Temp Probe SN FW Boot Function Last configured zero value System temperature of the electronics Name of the probe Serial number of the electronics Firmware number Bootloader number 6 Software Installation This section contains the technical requirements and procedures for installing the software Futura Tool 6 1 Futura Tool Futura Tool contains all of the configuration and calibration tools you will require to set up your BioPAT ViaMass system It includes functions for reading BioPAT ViaMass status information setting operating modes taking real time readings for system testing a diagnostics function to aid problem solving and the facility to upgrade BioPAT ViaMass firmware This section contains installation instructions for Futura Tool See Chapter 9 Using Futura Tool page 37 for usage instructions 6 2 Minimum System Requirements Microsoft Windows XP Service Pack 3 Microsoft Windows Vista or Microsoft Windows 7 1GB RAM or higher 1 6 GHz Intel Atom or higher At least 20 free hard drive space 1 GB minimum Atleast one free USB 2 0 port 6 3 Installing To set up Futura Tool on your PC you need to install Futura Tool Installer which is provided as part of your BioPAT ViaMass order Futura Tool requires Microsoft Net and FIDI drivers to be installed for it to work properly The installa
24. button CPM Factor in the submenu ViaMass CAP The submenu for entering the conversion factor opens BIOSTAT B Visitas L AP SUBST D2 AOE TE CM BASET 2 34 Measurement Options IMPORTANT gt Enter the conversion factor in the input field CPM Factor The conversion factor is used to convert the measured capacitance value into cells per milliliter C ml To determine this conversion factor you must draw one sample in the exponential phase of the process to be monitored and record the related capacitance value The conversion factor can be determined using the value determined by the laboratory in cells per milliliter and the recorded capacitance values The conversion factor CPM Factor is calculated with the help of the follwowing equation C M Fs Cell densit CPM Factor LA sapma Z Cceltdensity Reference value for cell density Cap 1 Capacity value from BioPAT ViaMass The biomass value BMass calculated using the CPM factor only correlates with the reference value for the density of living cell during the exponential phase In the sta tionary phase the capacitance value continues to rise because the volume of living cellular keeps growing although the cell density no longer does As a result of this the reference value for cell density no longer agrees with the biomass value The BioPAT ViaMass system determines the living cellular volume which only correlates with the ce
25. chemical effect at low frequencies called Polarization Therefore this mode uses a technology called Polarization Correction see section 8 5 Polarization Correction page 36 for technical details regarding this technology In this mode the biomass is measured at 0 6 MHz with polarization correction Noise filtering is at a medium level of 30 in this mode by default in some circum stances you can manually increase or reduce it as required Typically 0 1 pF cm resolution on the instrument represents 105 Cells ml 8 1 1 2 Bacterial Mode Bacterial Mode is optimized for bacteria cells e g E Coli which is around 3 microme ters in length In this mode the biomass is measured in dual frequency at 1 12 MHz and then 15 MHz Most cells are not polarized at 15 MHz so the 15 MHz reading is used as a form of auto zero and subtracted from the 1 12 MHz figure Therefore the capaci tance of background matter is automatically subtracted from the signal Noise filtering is at a high level of 90 in this mode by default although in some circumstances you can manually increase it or reduce it as required 0 1 pF cm resolution on the instrument will typically represent 109 Cells ml or 2 grams per liter 8 1 1 3 Microbial Mode Microbial Mode is optimized for microbe cells for example yeasts and fungi with a typical cell diameter of around 6 micrometers In this mode the biomass is measured at 0 6 MHz and then 15 MHz Cells like t
26. chluss am Trained and qualified personnel of the operator or Bioreaktor function test service technicians of Sartorius Stedim Biotech during servicing and mainte GmbH nance Operation Personnel who has read this documentation and is familiar with the operation of bioreactors Factory maintenance and Service Department of Sartorius Stedim Biotech service GmbH or the sensor manufacturer 2 3 Intended Usage The BioPAT ViaMass is a compact system for determining the living biomass in the concentration range and under the conditions as described in the technical data It is used for monitoring and evaluation of metabolic processes of biological processes e g cultivations 2 4 Limitations of Use and Misuse Possible damage to equipment The medium must not cause corrosion of the sensors Otherwise the reliability and lifetime of the sensors will be affected The BioPAT ViaMass sensors are not allowed to connect to amplifiers of third party vendors Sensors from other vendors are not allowed to connect to BioPAT ViaMass amplifiers 2 5 Electrical safety If the device is not used in accordance with the instructions this can affect the product liability for the device Make sure that the voltage rating printed on the AC adapter is identical to your local line voltage The wall outlet must have a protective grounding conductor Do not switch on the equipment if the AC adapter or power cord are damaged f the B
27. commend that the packaging be retained for use in the unlikely event that any item needs to be 4 2 2 Software Installation Do not connect your BioPAT ViaMass system to a PC until all required software has been installed The Futura Tool is required to configure your system and ascertain that it is functioning correctly It is also capable of data logging and real time measurement You may not need to install this if you are using our control system The Futura Tool installation also installs the drivers required to connect your BioPAT ViaMass system to your PC The Futura Tool must be installed prior to connecting the BioPAT ViaMass system to the PC as Futura Tool contains the drivers required to interface to the BioPAT ViaMass hardware If you are unsure about any steps in the installation process please refer to Chapter 6 Software Installation page 21 4 2 3 Connecting Probes and Cables It is easy to connect BioPAT ViaMass cables and probes Simply line up the colored dot on the connector with the colored dot on the socket and push in However the connectors feature a special ring lock device that will not allow them to be removed unless the correct technique is used as described below Plug collar The plug collar indicated must be pulled out a short distance the distance is almost imperceptible less than 1mm from the socket to disengage the lock Continue to pull on the plug collar to uncouple th
28. d distributor 7 4 1 Prerequisites 1 New firmware file supplied by Sartorius Stedim Biotech or your authorised distributor 2 A PC with Futura Tool installed see section 6 1 Futura Tool page 21 for instal lation instructions and at least one free USB 2 0 port 3 A BIoPAT ViaMass Connect Hub 1 connected to the above computer The BioPAT ViaMass in need of new firmware 5 Futura Bootloader version 234207p10 or higher see Firmware Upgrade Instructions below A If the Futura Bootloader version is less than p10 you will need to return your BioPAT ViaMass to Sartorius Stedim Biotech to perform the firmware upgrade 7 4 2 Firmware Upgrade Instructions 1 Connect the BioPAT ViaMass you wish to upgrade to your computer via the BioPAT ViaMass Connect Hub 1 You may have to wait for the hardware drivers to be installed if this is the first time connecting the equipment to your PC 2 Run the Futura Tool software On the Connect to Futura dialog choose search and wait for the computer to find your connected BioPAT ViaMass Select and connect to it Futura Tool will show you information about the BioPAT ViaMass BioPAT ViaMass System Components 25 26 BioPAT ViaMass System Components 3 Choose the Futura Updates tab and then click the Futura Firmware button This will show you the Firmware currently inside the BioPAT ViaMass and the controls needed to update it 4 Check that the Boot Loade
29. de the Measurement Settings pane in the Operating Modes tab allows you to select a measurement mode based on your application To change the Measurement Mode used press the Edit Measurement Mode button in the Measurement Settings area of the Operating Modes tab in the main Futura Tool window You should now see the following screen Bune nt Modes The available measurement modes are Cell culture Bacterial Microbial Signal simulator Custom These are described in detail in Chapter 8 Measurement Options page 28 Click the Save Changes button to confirm your selection A summary of your settings will be displayed in the Operating Modes tab in the main Futura Tool window 9 3 3 Clean and Zero Functions his section is only available when in Futura Connect Hardware Mode Clean and Zero Functions v Enable Hub Zero Button The Set Zero button sets the current capacitance value in BioPAT ViaMass as the zero value Clear Zero removes any zero value that was previously set See section 8 2 Zeroing page 33 for more details on zeroing your BioPAT ViaMass Clean Pulse is not available for the BioPAT ViaMass single use sensor Using Futura Tool 43 44 Live Readings Capacitance pF em 0 3 cordi non Using Futura Tool 9 3 4 Live Readings The Live Reading panel shows the output of BioPAT ViaMass with all Hardware and Measurement Settings applied I
30. e parallel proximity to cables associated with electronic variable speed motor controllers or inverters While the BioPAT ViaMass is immune to permissible levels of electrical interference some inverter installations radiate rather more than they should It is good practice to regularly check for cable integrity using the Signal Simulator for capacitance and conductivity If you see any significant deviation from the norm you should disconnect the BioPAT ViaMass from your system completely and request assistance from your distributor or Sartorius If during normal operation with the probe fully immersed in media and at opera tional temperature any of the following issues are observed immediately disconnect the BioPAT ViaMass from the probe and investigate The Conductivity is at a significantly different value than expected Any signs of galvanic corrosion are visible on the probe body or close to it Due to the electronic nature of the dielectrics measurement it is possible to have a fault condition where a small DC current is present on an electrode This manifests Itself as bubbles forming on the electrode when a Clean Pulse is not in progress In the unlikely event that you see bubbles under these conditions completely discon nect the BioPAT ViaMass from your system immediately to prevent any possible damage and request assistance from your distributor or Sartorius BioPAT ViaMass System Components 27 28 M
31. e plug from the socket For a cable hold the collar as shown and pull away from the socket 4 2 4 Connecting the Single Use Sensor Patch Fach disposable sensor has four platinum electrodes and a pair of plastic ears on the reverse of the sensor which locate in the front end sensor connector enclosure of the BioPAT ViaMass This enclosure features a locking ring which contains a guide groove that when rotated to the Lock position grips the locating tabs on the ears to hold the sensor patch securely onto the BioPAT ViaMass connector for a reliable connection To connect a disposable sensor patch first ensure that the locking ring on the connector is in the Unlock position Next line up the BioPAT ViaMass connector over the sensor Ensure that the sensor s ears align with the slots in the locking ring The probe can be fitted either way round Carefully place the BioPAT ViaMass connector squarely onto the sensor so that the tabs on the sensor s ears are just hidden by the connector s locking ring There should be a gap of approximately 3 mm between the probe and the BioPAT ViaMass connector Quick Setup Guide 13 14 Quick Setup Guide IMPORTANT Twist the locking ring fully round to the Lock position This will pull the probe securely onto the connector ready for use To release the disposable sensor simply rotate the locking ring to its Unlock position This will release and push off the disposable probe Alw
32. easurement Options 8 Measurement Options 8 1 Measurement Modes Description The BioPAT ViaMass can be used with a variety of different cell suspensions and Sartorius provides optimized preset measuring modes for common solutions A custom measurement mode is also provided for tailoring the BioPAT ViaMass to your exact requirements 8 1 1 Measurement Modes Available This section describes in further detail what happens inside the BioPAT ViaMass when each mode is being used Cells come in all shapes and sizes and respond differently when exposed to radio frequencies However they always fit a model around the beta dispersion explained in the theory section of this manual see Chapter 11 Detailed Theory of Operation page 49 There is also a set of filtering options that the BioPAT ViaMass system can be configured to use Some will suit highly aerated vessels others suit very thick suspensions etc If you want to select your own settings you can choose custom mode There is also a Signal Simulator mode which is used to test your BioPAT ViaMass system with the supplied Signal Simulators The operating modes are described below 8 1 1 1 Cell Culture Mode Cell Culture mode is optimized for larger cells such as animal or plant cells for example CHO cultivation with a diameter of approximately 10 micrometers All metal liquid boundary probes such as BioPAT ViaMass probes are affected by an unwanted electro
33. em with a BioPAT ViaMass Signal Simulator The Signal Simulator measurements will only be valid when it is measured in this mode You cannot change any settings in this mode Any zero that may be available is temporarily bypassed and if possible the measurement speed is increased in order to improve the response time 8 1 2 Selecting a Measurement Mode Modes can be selected using Futura Tool or a Sartorius control unit Modes set using Futura Tool or a control unit are stored in the BioPAT ViaMass and are used when calculating the measurements for Modbus and Current Loop outputs This allows two different sets of measurement modes to be configured one for the Modbus or Current Loop outputs for PLC control and the other for monitoring of the system using Futura Tool software Measurement Options 29 30 Measurement Options 8 1 2 1 Futura Tool Start the Futura Tool software connect to the BioPAT ViaMass probe combination desired in the usual manner and select the Operating Modes tab In order to change the Measurement Mode the BioPAT ViaMass must be configured to Futura Connect Hardware Mode If it is not already configure the BioPAT ViaMass to this Hardware Mode see section 9 3 1 Hardware Settings page 39 for details A Measurement Settings pane highlighted in the red box will now be displayed in the Operating Modes tab lule a los POR an LLIT G TUS Alero T Next click on the Edit Meas
34. en AES a S ireen i S ASA 50 TISPCOBOHOU VID sese e rasinis ees 52 1 About this Document 1 1 Function This operating manual describes the safe installation and use of the BioPAT ViaMass The operating personnel must read these instructions prior to operational startup These instructions should always be available at the workplace 1 2 Target Group These operating instructions are designed for qualified personnel Only qualified personnel may set up the BioPAT ViaMass connect it to the bioreactor or perform in process operation or maintenance 1 3 Notes on Using these Instructions All information in these operating instructions considers the state of the art the applicable standards and guidelines and our many years of experience and know ledge These operating instructions provide you with all the information necessary to install and operate the BioPAT ViaMass The operating instructions must be read and used by all persons entrusted with the operation service cleaning and troubleshooting of the device This applies particu larly to the safety instructions listed Please read these operating instructions carefully and completely before using this device These instructions are part of the product Keep them in a safe and easily acces sible place near the device s site of installation fthe instructions should be lost or misplaced you can order new ones 1 4 Symbols Used As a means of instruction
35. g traditional reusable fermenter equipment But industrial cell cultivation tends more and more to single use SU fermentation solutions In order to follow these an easy to use online biomass monitoring system is a basic necessity BioPAT ViaMass is the first standardized online biomass measurement solution for single use fermenter systems which is fully integrated into the standard fermenter control system and tailored to the single use fermentation bags such as the Flexsafe RM and Flex safe STR 3 1 Concepts The BioPAT ViaMass system measures an electrical property of cell suspensions called Capacitance Academic and industrial research has demonstrated a correlation between this property and live biomass A detailed theory of operation can be found in chapter 11 Detailed Theory of Operation page 49 Electrical capacitance is measured in the SI unit farads although picofarads are more common in practical use For cell suspensions capacitance is usually quoted in pF cm and as a rule of thumb one pF cm equates to one gram per litre of dry weight for microbial or 1x106 cells per milliliter of live cells for cell culture The BioPAT ViaMass patented technology converts capacitance into a live biomass reading normally cells ml or g l for spun solids dry weight However other units can be derived from the raw capacitance measurements these may be more relevant to the chosen application and are covered later in the manual You
36. h a sensor patch The only difference is that the locking procedure is not needed to establish the connection Once you have created the connection to your BioPAT ViaMass and the DCU is ready for operation switch to the calibration mode Press the touch button CAP 7Z in the Calibration menu The submenu ViaMass CAP 7 opens P Press the touch button Measure in the submenu ViaMass CAP Z BIOSTAT B Visitas L amp P SUBST Ca LA B T a a L BASET 2 oi ViaMass CAP 1 Mode gt Press the touch button Signal Test in the submenu ViaMass CAP Mode ViaMass Signal Simulation CAP 1 x Connect high Signal Simulator 47 SpFicm 14 STS m ViaMass Signal Simulation CAP 1 x Connect kw Signal Simulator 97 BpF em 33 riem The submenu ViaMass Signal Simulation CAP for checking the BioPAT ViaMass electronics opens gt Connect the signal simulator for the high value to the electronics As soon as the electronics detect the high simulator the capacity reading appears in the Cap field and conductivity in the Cond field If the expected measurement values are within the acceptable range see the follow ing table then they are displayed in black gt Confirm this check by pressing the ok key If the measurement values are not within the acceptable range the results are displayed in red In this case check whether you have connected the right high simu lator that
37. hese do not give much biomass reading at 15 MHz so the 15 MHz reading is used as a form of auto zero and subtracted from the 0 6 MHz figure Therefore the capacitance of background matter is automatically subtracted from the signal Noise filtering is at a high level of 90 in this mode by default although in some circumstances you can manually increase or reduce it as required All metal liquid boundary probes such as BioPAT ViaMass probes are affected by an unwanted electrochemical effect at low frequencies called Polarization Therefore this mode uses a technology called Polarization Correction see section 8 5 Polarization Correction page 36 for technical details regarding this technology 0 1 pF cm resolution on the instrument typically represents 106 Cells ml or 0 5 grams per litre 8 1 1 4 Custom Mode The default settings for Custom mode are single frequency measurement at 1 MHz no polarization correction no dual frequency and low filtering You can adjust any settings in this mode It is mainly used for experimentation and with guidance from Sartorius support staff 8 1 1 5 Signal Simulator Mode The signal simulator mode is only available in the Futura Tool software For checking the electronics with signal simulators during a DCU integration please read sec tion 5 2 System Check with DCU Integration page 18 This mode should be used when you are checking the operation of your BioPAT ViaMass syst
38. ied number of readings has been taken the upper 5 and lower 595 magnitude readings are removed before the mean average is taken This creates an suppression of interfering signals 8 6 1 Rocking Motion Monitoring The rocking motion of the rocker causes signal fluctuations of the measurement signal due to the variation of the liquid level over the sensor in the Flexsafe RM bag For this reason appropriate optimization filters are implemented in the system s electronics including different rocking motion parameters Using these filters enables the biomass evolution monitoring continuously in rocking motion cell cultivation When in Rocker Bag Mode Flexsafe RM Bag the BioPAT ViaMass filters out the variation in readings caused by the probe electrodes becoming uncovered due to the bag rocking back and forth Only use this filter if you are measuring in a situation where the liquid level above the electrodes varies and is 3cm or less 9 Using Futura Tool 9 1 Connecting to BioPAT ViaMass First plug in your BioPAT ViaMass to a BioPAT ViaMass Connect Hub and connect this to your PC via USB Click on the Futura Tool icon on the desktop or launch the program from the start menu Start gt Programs gt Aber Instruments gt FuturaTool gt Futuralool IET IT IR iaaii LTTTTTTTTTTTTTTTTTTTTITITTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTITTITITIIT The Connection screen is displayed automatically when Futura Tool is launched Use
39. ioPAT ViaMass itself the AC adapter or any cables of the system are dama ged disconnect the equipment from the power supply and secure it that it cannot be turned on Chemicals e g gases or liquids that can corrode and damage the inside or outside of the BioPAT ViaMass AC adapter or any cable or peripherals must be kept away from the equipment Safety Instructions 7 Installation Notes The operator shall be solely responsible for any modifications to the equipment and for connecting any cables or equipment not supplied by Sartorius Information on operational quality is available upon request from Sartorius You should only use peripherals and options supplied by Sartorius Safety Instructions 3 Introduction One of the most requested parameter in industrial cell cultivation is the monitoring of biomass The knowledge of the biomass progress during a fermentation process gives deeper process knowledge and understanding Therefore it enables the control of the biomass and helps to define feeding harvesting or infection points Offline methods like visual cell counting or semi automated systems still dominate the biomass measurement in industrial cell cultivation But these offline methods based on taking a representative sample cannot monitor the process continuously The radio frequency RF impedance method for online in situ detection of viable biomass has already become well established in biopharmaceutical applications usin
40. ise Sartorius stedim biotech Operating Instructions BIoPAT ViaMass TTE 85037 544 92 Vers 10 2014 Contents 1 About this Document 000 akiai nnn ne 5 NEU AUDERENT 5 NAME utn HO 5 1 3 Notes on Using these Instructions aaa 5 14 Symbols co siete cana ttn ai r dr i as i e r I a ieS 5 15 Stru etute OT thelVIdlttdl esci aus e as a ns tanked EEan a d aA 6 2 Sarety WISUUCHONS ins e imi ii duce dme a d S i i tmb tur A 7 2 1 General WATOM Ma LO oe Kaina denm G ii a Rar S i RE Rei i i 7 2 2 AUCIOLIZ ICE DIN Lens as nas a as a ud k a S o dunes 7 2 3 Intended SAO ssc duction phan i a i nio E INTO iid aud M S ee ao 7 2 4 Limitations of Use and MISUSE sim ania is iai ER a ROS GH RU rs 7 25 NCCE ICA SATCU cc oi re ae i iai meade ed PONE EVEN RE i E ia 7 3 Introduction RETE 9 UNE USC serrera velai iais K ais io a i e a a ENES 9 3 2 System Components Overview essentiam asas ia natur i a i i 10 3 2 1 Probe sm 455 rei ra ak i ns dard i i a in 10 3 2 BioPAT ViaMass Connect Hub 10 3 2 3 Software I si k o a ub a i a a E ad RACE ub dcr aa 10 Des EU WOO amor aes 11 4 Quick Setup Guide annann nananana akiai rennan rrera ia iais 12 4 1 Typical System Checklist Lk sias rs i i a a Rr i a 12 4 2 Getting Started airis o saaa i S S oo ni a aa a S ia 12 Mino ERE 12 4 2 2 Software Installation aaa aaa kaka een ene ene 12
41. ites The conductance signal itself may provide useful information about the metabolic state of the fermenter an area of study that is at present relatively unexplored Changes in o will also affect the characteristic frequency but under the conditions used little effect is likely upon the value of e for constant biomass as measured at a suitable constant frequency In some situations using the Dual Frequency measurement mode reduces undesir able variation in the derived biomass signal due to f e changes The BioPAT ViaMass will accurately measure capacitance in media with conductivity up to 40 mS cm Values up to 80 mS cm require a different probe also providing up to 800 pF cm capacitance range and this can be supplied if required Sartorius Stedim Biotech GmbH August Spindler Strasse 11 37079 Goettingen Phone 49 551 308 0 Fax 49 551 308 3289 www sartorius stedim com Copyright by Sartorius Stedim Biotech GmbH Goettingen Germany No part of this publication may be reprinted or translated in any form or by any means without prior written permission from Sartorius All rights reserved by Sartorius in accordance with copyright law The information and figures contained in these instructions correspond to the version date specified below Sartorius reserves the right to make changes to the technology features specifications and design of the equipment without notice Date October 2014 Sartorius Stedim
42. l operating range of 3 mS cm to 40 mS cm the BioPAT ViaMass gives readings in proportion to the total volume of intact cells in the sample The BioPAT ViaMass can be calibrated to translate this into other measurement units such as grams per liter cells per ml spun solids 9o or PMV 11 1 The Electrical Properties of Living Systems If anything is placed between two electrodes with an alternating voltage applied to the electrodes one may characterise the so called passive electrical properties of the system by two variables the electrical capacitance C and conductance G In SI units capacitance is measured in farads F and conductance in siemens S In biological work the values of interest are usually in the picofarad pF and milliSie mens mS ranges The capacitance and conductance of typical ionic solutions are for practical purposes independent of frequency between DC and 1 GHz If the elec trodes are plane parallel plates of area A separated by a distance d the capacitance and conductance measured will be a function of both A and d The intrinsic properties of interest which characterise the material are its relative permittivity e and its conductivity o which are related to C and G by 1 o G d A 2 C d Agg Where d A has the units of cm and is known as the cell constant K and e is known as the permittivity of free space and is equal to the capacitance of a 1 cm cube containing a vacuum
43. l Simulators supplied are used to check the system is functioning correctly A Signal Simulator pair consists of a high and zero value to test the full range of the BioPAT ViaMass When using a BioPAT ViaMass system the BioPAT ViaMass Signal Simulators Sartorius part number BPV0011 must be used Plug these simulators in the BioPAT ViaMass connector in the same way you do it with a sensor patch The only difference Is that the locking procedure is not needed to establish the connection However the BioPAT ViaMass Signal Simulators require a different calibration file to operate correctly Disposable probes do not contain the memory required for storing settings and calibration files This Signal Simulator calibration file is instead stored in the BioPAT ViaMass electronics and is selected automatically when the BioPAT ViaMass measures a conductivity of greater than 36 mS cm and detects that a 2 terminal device is connected such as a Signal Simulator but unlike a probe The high value Signal Simulator must therefore be inserted first in order to trigger this mode change The Signal Simulator mode will remain active for 90 seconds during which time a value using the zero value Signal Simulator must also be recorded The BioPAT ViaMass will then revert to its previous mode Connecting the BioPAT ViaMass to Futura Tool software will cause both BioPAT ViaMass and soft ware will automatically switch to their respective Signal Si
44. ll density in the exponential phase 8 4 Settings for Alarm Limits P Press the touch button CAP 7Z in the Main menu The submenu Alarm CAP opens BIOSTAT B 2014 07 28 Su sT oF Dos ALIIT a BT OQ ik ti P Set the upper and lower alarm limit according to your application P Switch the alarm for the capacitance value on or off Measurement Options 95 36 Measurement Options 8 5 Polarization Correction This correction is used to correct an electrochemical effect that occurs with all metal liquid boundary probes This affects low frequency capacitance readings The capacitance versus frequency curve looks similar to a 1 x 2 curve almost asymptotic at low frequency settling to a horizontal straight line at high frequency In order to compensate for this two additional biomass measurements are made at low frequencies and from this the shape of the curve can be estimated The offset added by this effect is then subtracted from the actual biomass measurement This removes the effect of changing polarization on the biomass signal 8 6 Filters BioPAT ViaMass includes the facility to output measurements based on the mean average of a number of readings This filter is accessible from Futura Tool software and over Modbus from a control unit A filter value of O or 1 equates to no filtering while a value of 180 maximum takes the mean average of 180 readings Once the specif
45. lluminates red the BioPAT ViaMass electronics is probably in the incorrect hardware mode Use Futura Tool Chapter 9 Using Futura Tool page 37 to change the Hardware Mode if required You are now ready to connect your BioPAT ViaMass system to a PC or system controller 4 2 7 Connecting Your BioPAT ViaMass to a PC Once Futura Tool has been successfully installed Software Installation section above take the USB cable provided and connect it between the orange USB socket on the BioPAT ViaMass Connect Hub 1 and a spare USB port on your PC If connecting the BioPAT ViaMass Connect Hub 1 to your computer for the first time please allow up to a minute for it to be registered by the PC before attempting to connect to the device using Futura software This extended hardware discovery time is only necessary when connecting your BioPAT ViaMass Connect Hub 1 to your PC for the first time 4 2 8 Assembly Connection of BioPAT ViaMass Elektronics to a BIOSTAT RM gt Ensure that the equipotential bonding cable is connected to the electronics bracket as pictured below gt Connect the equipotential bonding cable to the equipotential bonding point at the control unit or your local installation P Snap the electronics into the illustrated holder It should be retained as shown in the picture Connect the data cable to the electronics unit Quick Setup Guide 15 16 Quick Setup Guide 4 2 9 First start up with con
46. matches your electronics unit If the value is not within the acceptable range despite the correct simulators please contact your local Sartorius service part ner Signal simulator BioPAT ViaMass Readings Capacitance Conductivity Capacitance pF em Conductivity mS cm 100 40 100 3 40 1 0 0 0 1 0 0 5 b After confirming the high simulator value repeat the procedure with the signal simulator for the zero value zero or low value As soon as the low simulator is detected by the electronics the capacitance reading appears in the field Cap and the conductivity reading in the field Cond If the readings are in the acceptable range they are displayed in black gt Confirm this check by pressing the ok key If the measurement values are not within the acceptable range the results are displayed in red In this case check whether you have connected the right low simu lator that matches your electronics unit If the value is not within the acceptable range despite the correct simulators please contact your local Sartorius service part ner System Check 19 ViaMass CAP 1 Into 20 HSMA SM 13011 LRK 1ZE MAS 234207p10 System Check 5 3 Displaying System Information on the BioPAT ViaMass To display the system data of your BioPAT ViaMass proceed as follows Press the touch button CAP in the Calibration menu The submenu ViaMass CAP opens Press the touch button Info in the su
47. mulator modes BioPAT ViaMass firmware 2335 07 118 onwards Note that the BioPAT ViaMass will remain in this mode regardless of what is connected to it You must therefore ensure that it is reverted to the standard operational mode when finished with the Signal Simulators since the Signal Simulator calibration file will give incorrect readings with real disposable probes System Check 17 18 System Check BioPAT ViaMass Signal Simulators Use with BioPAT ViaMass Directions Plug in switch on the power and wait until the Capacitance reading is steady allow at least 20 minutes warm up time BioPAT ViaMass run hot so do not be alarmed Check that the Simulator settings give the correct display readings as below First with the 100 pF cm 40 mS cm simulator followed by O pF cm 0 mS cm Signal Simulator BioPAT ViaMass Readings Capacitance Conductivity Capacitance pF em Conductivity mS cm 100 40 100 3 40 1 0 0 0 1 0 0 5 5 2 System Check with DCU Integration Both accompanying signal simulators can be used to check whether the system is functioning correctly A signal simulator pair consists of one very high value and a zero value or low value for checking the entire BioPAT ViaMass measuring range When using a BioPAT ViaMass system the BioPAT ViaMass Signal Simulators Sartorius part number BPV0011 must be used Plug these simulators in the BioPAT ViaMass connector in the same way you do it wit
48. of Functions on this tab can cause permanent damage En Futura bardware Vh Fares Ligne Boot Loader Verio 24207610 lintea re Viersian 207g Firmware Update File The Futura Updates tab facilitates updating the firmware of a connected BioPAT ViaMass Click the Futura Firmware button to activate the update screen Click the Browse button and carefully select the upgrade file for the BioPAT ViaMass then click Update Futura Tool will then perform the update unaided In order to perform a firmware update the existing Bootloader version must be 234207p10 or higher If the Futura Bootloader version is less than p10 you will need to return your Futura to Sartorius Stedim Biotech to perform the firmware upgrade Do not unplug the BioPAT ViaMass or any other BioPAT ViaMass hardware during the update If for any reason the update fails you can try again A BioPAT ViaMass with no firmware or which has a failed firmware update cannot be used Before starting it is important to ensure that the update file is a valid BioPAT ViaMass firmware update If Futura Tool is started and connected to a BioPAT ViaMass that has no firmware or has experienced a failed update it will only display the Futura Updates tab The BioPAT ViaMass can then be updated using valid firmware If this completes successfully the BioPAT ViaMass will be usable again if it does not complete contact Sartorius Stedim Biotech for further information
49. other BioPAT ViaMass found in the search will be released for use by other applications Using Futura Tool 37 9 1 3 Futura Tool Menu The Futura menu has two entries The first is Disconnect Connect which enables Futuralool HARS07014 connection and disconnection of a single BioPAT ViaMass The second item is Futura E p Reset which reinitializes the connected BioPAT ViaMass by forcing the firmware to File Buc Help reboot without physically disconnecting the BioPAT ViaMass Disconnect Futura Status Probe Status 9 1 4 Futura Status Tab When a BioPAT ViaMass has been successfully connected to Futura Tool the software will display the Futura Status tab Other accessible tabs are Probe Status Operating Modes Quick Readings Advanced Diagnostics and Futura Updates all of which are explained later in further detail The Futura Status tab displays details about the currently connected BioPAT ViaMass including the Serial Number and Part Number of the BioPAT ViaMass what type of BioPAT ViaMass it is the date it was made the minimum and maximum temperatures that the BioPAT ViaMass has internally experienced so far your BioPAT ViaMass can run very hot so do not be alarmed This tab also displays other details which provide information regarding the connected BioPAT ViaMass state and history Freee 15 40 01 HHS HHE Be Futura Hep Connecbed to HAS TRE Cano larry
50. r BioPAT ViaMass also measures the conductivity of the medium in millisiemens per centimeter mS cm Conductivity is not used to measure biomass but is indicative of the production or utilization of ions by the cell suspension e g pH control and other fermentation processes Introduction 9 10 Introduction 3 2 System Components Overview The BioPAT ViaMass system consists of the electronics as the main measurement and evaluation unit and the sensor connector which also has an amplifier on board In order to complete the measurement system the electronics is connected to the single use sensing patch Figure 1 BioPAT ViaMass sensor patch Figure 2 BioPAT ViaMass electronics connected to the patch connector 3 2 1 Probe The probe is the part of the instrument that is placed into the solution under test It can be physically separated from the rest of the system for cleaning or replace ment 3 2 2 BioPAT ViaMass Connect Hub BioPAT ViaMass Connect Hub 1 allows the connection of a BioPAT ViaMass probe sensor patch in combination with the corresponding electronics For the connected BioPAT ViaMass the BioPAT ViaMass Connect Hub can simultaneously output Current Loop and Modbus RTU signals for use with PLCs and other control systems whilst optionally interfacing with a PC over a single USB cable allowing independent control calibration monitoring and logging of the BioPAT ViaMass probe connected to the sys
51. r Version ends in p10 or higher If the version number is lower than p10 disregard the remainder of these instructions as you will need to return the BioPAT ViaMass to Sartorius Stedim Biotech to perform the firmware upgrade 5 Click Browse and carefully choose the new firmware file file extension bin Then click Update and wait for the process to finish 6 Close Futura Tool Then unplug your BioPAT ViaMass from the hub wait a few seconds and plug it back in again This power cycles the BioPAT ViaMass 7 Start Futura Tool again and connect to the BioPAT ViaMass The Firmware section will show you that the BioPAT ViaMass has the new version inside it If you require assistance please contact Sartorius Stedim Biotech or your authorized distributor 7 5 Earthing the BioPAT ViaMass Electro magnetic interference from nearby electrical machinery can manifest as noise in the BioPAT ViaMass readings The Capacitance measurement is especially susceptible to this In order to minimize measurement error the BioPAT ViaMass casing has to be securely bonded to the dedicated plant ground using the supplied earth strap cable The BioPAT ViaMass electronics and probes should be located as far away as possible from any machinery likely to cause electrical interference such as large motors The graph below shows the difference between a poorly and well earthed system Capacitance pi cm Ground strap removed
52. ra Tool 8 2 1 Zeroing Using Futura Tool Connect to your BioPAT ViaMass using Futura Tool in the usual manner Navigate to the Operating Modes tab Press the Set Zero button in the Clean and Zero Functions section of the screen Clean and Zero Functions v Enable Hub Zero Button Measurement Options 33 8 2 2 Zeroing Readings on the DCU P Press the touch button CAP in the Calibration menu The submenu ViaMass CAP opens P Press the touch button Measure in the submenu ViaMass CAP ViaMass CAP 1 Mode P Press the touch button Zero Now in the submenu CAP 2 Mode The submenu ViaMass CAP for zeroing the BioPAT ViaMass electronics appears ViaMass CAP 1 P Press the Set Zero button Next the field for the capacitance value Cap shows the zero value The Zero field displays the current offset also called the deduction value his offset is the value that is deducted from the respectively running measure ment P Pressing the Clear Zero button deletes the offset value and the current offset value of the system is displayed in the field Cap Unlike when zeroing the Futura Tool software the DCU remembers the offset value so that the last pre zeroing status is not lost 8 3 Converting the Capacitance Value to Cells per Milliliter C ml P Press the touch button CAP in the Calibration menu The submenu ViaMass CAP opens Press the touch
53. rating Modes tab above are not used pura Hec Connected ta HASO9009 Futura Status Peobe Suus Opening Modes Quick Resting Advanced Dapoer Futura Updates i Capacitance pF cm 111 08 Conductivity mS cm 39 86 Enable the Continuous Readings checkbox to make the application repeat readings automatically Checking the Enable Logging checkbox will automatically prompt for a filename under which to save the readings Once you have selected a filename start logging by pressing the Get Reading button Enable the Continuous Readings checkbox before pressing the Get Reading button if you wish to log multiple readings Logging produces a CSV file with the following format Time Stamp Run Time Decimal Hours Capacitance pF cm Conductivity mS cm Measuring Frequency KHz Dual Frequency High Frequency KHz Capacitance at Measure Frequency pF cm Capacitance at High Frequency pF cm Conductivity at High Frequency mS cm Some fields are not populated depending on the settings used e g High Frequency is left blank when Dual Frequency is off Press the Stop Timed Reading button at any point to stop the readings and logging if enabled Using Futura Tool 45 46 Using Futura Tool IMPORTANT 9 5 Futura Updates Tab Futuralool HASOTOOT en CONTE Futura Stans Pebe Status Opreg Modes Quick fades Adwanced Gagro Futura Upiti SCE TAT Raler to Klara al improper ure
54. surement Mode in the submenu CAP Mode The submenu Mode for selecting measurement modes opens see section 8 1 1 Measurement Modes Available page 28 P Select the measurement mode that suits your application Measurement Options 31 ViaMass CAP 1 Settings ViaMass CAP 1 Settings Bacher d Mods Frigus cy 1 12 DKFLr Meving Average an Dua Frequency ON High Frequency 15650kHz Pal Coreis Ha Po C ViaMass CAP 1 Settings 32 Measurement Options Cell Culture Mode The submenu Cell Culture Mode displays the configurations for the cell culture mode To use the BioPAT ViaMass in a BIOSTAT RM you must activate the rocker filter gt Touch the button Rocker Filter The field now displays the value ON gt Confirm these changes with ok Bacterial Mode The submenu Bacterial Mode displays the configurations for the bacterial mode To use the BioPAT ViaMass in a BIOSTAT RM you must activate the rocker filter P Touch the button Rocker Filter The field now displays the value ON gt Confirm these changes with ok Microbial Mode The submenu Microbial Mode displays the configurations for the microbial mode To use the BioPAT ViaMass in a BIOSTAT RM you must activate the rocker filter P Touch the button Rocker Filter The field now displays the value ON gt Confirm these changes with ok IMPORTANT ViaMass CAP 1 Settings Moving Average
55. t is a useful tool when tuning Measuring Modes problem solving and matching values to a PLC To log readings taken with the Live Readings feature select the data logging interval using the Log Filter then click the Start Logging button in the Log Live Readings area This will ask you for a filename to store the readings Logging will start as soon as the Save button is pressed Logging produces a CSV file with the following format Time Stamp Run Time Decimal Hours Biomass Capacitance pF cm Conductivity mS cm Capacitance Zero Value pF cm Messages Dual Frequency Polarization Correction Filter Measuring Frequency KHz High Frequency KHz It is also possible to recall previously logged data by clicking on the View Log Data on Graph button in the Live Readings pane Navigate to the previously saved csv file and click Open in the file dialog to view the data on a graph Logge dDataGraph Start Tire 18 07 2011 21 42 37 End Time 15 07 2011 21 43 41 Logged Data 42 45 43 00 4315 43 30 Time 9 4 Quick Readings Tab his tab enables you to get quick capacitance and conductivity readings from the BioPAT ViaMass This is typically used for system checking and problem solving and may be used to generate log files to send to Sartorius Stedim Biotech Both Single Frequency and Dual Frequency Modes are supported The Measurement Settings stored in the BioPAT ViaMass itself configured in the Ope
56. t or kinked If placed into a rocking system it is recommended that you use the provided holder for the electronics so the movements have an effect on the replaceable extension cable 7 1 BioPAT ViaMass Care and Maintenance This section describes how to care for and maintain the BioPAT ViaMass system IMPORTANT IMPORTANT Comet bo Futura each COM gd aimes CIA rit ITM 7 2 How to Store Your BioPAT ViaMass If you need to store your BioPAT ViaMass for any significant time place it in its original protective packaging and store it in a cool dry dark place Do not drop any of the BioPAT ViaMass components as an impact onto a hard surface could damage them Keep all parts away from contamination 7 3 How to Clean Your BioPAT ViaMass Ensure items are completely disconnected before cleaning It is very important to disconnect all cables in order to reduce the risk of electric shock Use a soft slightly damp cloth to carefully clean any parts Do not use any type of abrasive pad alkaline cleaner scouring powder solvent or other cleaning agents These may damage the enclosures Please refer to section 7 6 BioPAT ViaMass Cables page 27 for cable care instruc tions 7 4 Firmware Upgrades Below are instructions to update the firmware in your BioPAT ViaMass You should only update the firmware in your BioPAT ViaMass if you have been instructed to do so by Sartorius Stedim Biotech or your authorize
57. tem using BioPAT ViaMass software Modbus and Current Loop signals from the BioPAT ViaMass Connect Hub provide third party control systems e g PLCs with both capacitance and conductivity readings for evaluation in control functions Once configured using BioPAT ViaMass software the BioPAT ViaMass modules can be operated independently without need ing to be connected to a PC In addition to capacitance and conductivity readings Modbus also has the facility to provide a viable biomass reading and offers control and configuration over the system 3 2 3 Software You should have received a USB flash drive with your BioPAT ViaMass system This contains a copy of Futura Tool for system configuration and data recording If this is missing from your package please contact Sartorius Stedim Biotech or your authorized distributor for assistance 3 2 3 1 Futura Tool Futura Tool is required to configure your BioPAT ViaMass system for use It provides details on the BioPAT ViaMass system calibration and operating mode options a quick readings mode for ascertaining operating status and other advanced functions mpg LET For further details on Futura Tool including system requirements and installation instructions please refer to section 6 1 Futura Tool page 21 Introduction 11 12 Quick Setup Guide IMPORTANT 4 Quick Setup Guide This section assumes the user is familiar with BioPAT ViaMass technology
58. tion software will check if those are installed and if not it will install them The pre requisite installa tion wizard will only be activated if the installation software cannot find previously installed software or if the previous prerequisites are out of date Follow the instruc tions on the installation software wizard Thess ace sel ini ended lup Che appdscanon 62 vun Ck on be che be ri Va a ered pai i tye iod dll gt bee Welcome to the Prerequisites Wizard The gj has donee Bed Ir dicm gd de El FIER Era Pe ci pr mi w hg Pa imm prakxizdac mpeg ks rea Ee pragesen ane fus sipand will acest paa in geting arc ribal oq cid poariquridbki Check Abert be gor Poe D dg bat ef creed Deck Pleki ai sr toe ir come etely whic ihe I EE Ting MITT Ek pi 1 jrr Harkinai Te birg or ce i r ABER INSTRUMENTS SOFTWARE LEHTE AGREEMEMT RPT STEE THA ELA t ONTARA T Pee TU Darri F TARRE AKCHOR COMPLETE THE ESTALLATICN CPL TH AG RST pr eH LOAD THE er ARG AMOR CECENI a Fi CABLE Phe TO COMPLETE THE eee Lay Pee ee ai COMES T THE TERME PF TER PC A UL UE TOUR UNO MENT FP OO RST VH TS apoE 0 THE AGREE 2REEn ADEE BOUND AL CET TERVE ND CONDONE EL FRECFRATE BOTT TITAS EL THIS SAT ALAM PMC EEA D HOT NIITLL OF E TH oF eg 2 A Le ASP HT HOT A EA ES A iT Ee ee ein CV darer accept Eas Fara ir te ices beer Software Installation 21 22 Software Installation 6 3 1 Microsoft Net Framework 4 0 Fut
59. tool software please check the following Ensure that the BioPAT ViaMass is securely connected to the BioPAT ViaMass Connect Hub Ensure that the BioPAT ViaMass Connect Hub is connected to its power supply and that the mains supply is switched on Ensure that the USB cable is correctly inserted into the BioPAT ViaMass Connect Hub Ensure that the USB cable is correctly inserted into a USB socket on your computer Ensure that the drivers and Futura tool software are correctly installed on your computer see Chapter 6 Software Installation page 21 Ensure that the probe is securely connected to the BioPAT ViaMass electronics 11 Detailed Theory of Operation The instrument works by measuring the electrical capacitance of the cell suspension at various radio frequencies The capacitance is strongly affected by the presence of viable cells with an intact electrically insulating outer membrane envelope Cells with leaky membranes and other non cellular solids have a much smaller influence on the capacitance of the suspension The illustrations above show a cell with an intact membrane viable cells only and the BioPAT ViaMass s electric field charging the membrane The charge on the membrane Is similar to that found on an electrical capacitor and this principle is used to detect and measure the presence of viable cells Provided that some measurement conditions are satisfied conductivity in the norma
60. trol unit After the BioPAT ViaMass electronics is connected to the control unit and is supplied with power it requires a warm up time of about 30 minutes P Press the touch button CAP in the Calibration menu The submenu ViaMass CAP opens BIOSTAT B Via azs GAP 1 Medo While Waiting for stable temperature is displayed in the ViaMass CAP submenu the electronics is in the warm up phase The rotating clock indicates that the warm up phase is running ee fuus deb scares Erud bu E Ato Cages Ul fm PLES 5 System Check This chapter outlines the steps required to check that your BioPAT ViaMass system is functioning correctly and assumes the software mentioned is installed and working You can perform these tests using Futura or if included in your system our local control unit Tests using the supplied Signal Simulators in a standard conductivity solution and with living biomass are detailed on the following pages 5 1 Using Futura Tool After the BioPAT ViaMass was connected click on the Quick Readings tab Set the measuring frequency to 1000 kHz single frequency and the Probe Calibration to Factory Cal Check the Continuous Readings checkbox then press the Get Reading button to start taking readings You are now ready to perform the checks detailed in the remainder of this chapter Once you have finished checking the system press the Stop Timed Reading button The two Signa
61. unit membrane enclosed volume of biological cells is not thought to be greatly dependent upon physiological status and since all cells need an intact cytoplasmic membrane to divide an operational definition of biomass as bio volume is appropriate The BioPAT ViaMass effectively measures biovolume i e the volume fraction P of the suspension which is enclosed by the cytoplasmic membrane of the cells Pis therefore dimensionless being ml enclosed by the cytoplasmic membrane per ml of cell suspension For spherical cells the relationship between the permittivity at a frequency of measurement low with respect to f and the volume fraction P is 9Pr Cn 4E 6 el el np Since r C and e are constants for a given set of conditions e and hence the measured capacitance C is linear with P and after subtraction of the background 5 IS directly proportional to P At values of P above about 0 2 and up to about 0 5 electronic correction is necessary to maintain linearity The dielectric increment measured will also depend upon the mean radii of the cells under study Typically yeast cells will produce a capacitance of 3 to 6 times that of an equal number of bacteria cells Similarly animal cells generate a capacitance of 8 to 16 times that of bacteria cells due to their larger mean radius For non spherical cells the factor 9 4 will differ and the appropriate radius to consider is that of a sphere of equivalent volume
62. ura Tool requires Microsoft Net 4 0 framework The installation software will check if the correct version of the framework is installed and if it is not found the installation software will provide instructions on how to install it The framework is included in the installation software so an active internet connection is not required Tick the box when you have read the terms and conditions of the Microsoft Soft ware click on Install and the installation process will begin Once the installation is complete click on Finish and Wizard FTDI x 32 Setup should appear click Next It is highly recommended that you make sure that the latest Net 4 0 framework updates and service packs that Microsoft provide are installed Consult the Windows manual on keeping the operating system and related applications up to date ii iai ee a d Ta ek es me T TO eg aT Ta eee A Tia ETE IE m4 E PROC RN CARPET PRU TS BORRAR UI Kia as me mi mai AS Pierii Tais rd De ad nin Ier TA en Lem MADEE s rou Dao rise to boi e paite O eri a Pee ee Ln erie A E r 6 3 2 FTDI Drivers The installation software will check if FTDI virtual COM port drivers supplied by Sartorius Stedim Biotech have been installed and will not continue until they are installed No user information is required simply follow the installation wizard to install the drivers The installation software will add FIDI x 32 application entry on 32 bit Windows and add FIDI x 64 applic
63. urement Mode button to display the Measurement Modes dialog You should see something like this a i S au T E Fevan ares Charges butcr to get raram etra ios Phan Futura Dua frepar eratis Use the Measurement Mode drop down list to select from the preset Measurement Modes Signal Simulator Mode or Custom Mode The Measuring Frequency Dual Frequency Polarization Correction and Filtering options will be initialized and editable according to the chosen Measurement Mode see section 8 1 1 Measure ment Modes Available page 28 These settings will be stored in the BioPAT ViaMass itself and used for both Modbus and Current Loop output measurements when connected to a BioPAT ViaMass Connect Hub Current Loops only ViaMamss CAP 1 Made ViaMass CAP 1 Measurement Mode 8 1 2 2 Selecting the Measurement Mode via DCU In the following you will find recommendations for configuring the system parameters as pre configured settings You can also adapt the system parameters to your application via the Customer Mode Once you have created the connection to your BioPAT ViaMass and the DCU is ready for operation switch to the calibration mode P Press the touch button CAP in the Calibration menu The submenu ViaMass CAP opens P Press the touch button Measure in the submenu ViaMass CAP BIOSTAT B Visitas L AP 1 SUBST Ca LA NCLIT 2 CH BASETZ m P Press the touch button Mea
64. use Current Loops enter the capacitance and conductivity values that will corre spond to currents of 4 mA and 20 mA in the Current Loop Settings area The current through the Current Loop outputs will then vary linearly in proportion to the measured capacitance and conductivity values maca Conductraty Offset Tres Lese f IG bes The Advanced Current Loop Settings will enable you to choose whether you wish to allow currents less than 4 mA or greater than 20 mA This will depend on whether the control system reading the Current Loops is tolerant to currents outside of these limits The Offset Trim and Gain Trim for both Capacitance and Conductivity are also configurable in the Advanced Current Loop Settings area These allow you to alter the offset and gain of the Current Loop outputs in relation to the actual capacitance and conductivity values in a yamx c fashion m being gain and c being the offset Conductivity Capacitance Conductivity uL a e sj l c tu s e Current Current This is a useful feature if trying to adjust the outputs of two or more BioPAT ViaMass to match one another Although in practice the Current Loop outputs of two identical BioPAT ViaMass are typically within two decimal places of each other with no correction applied To use the Modbus interface enter the baud rate and parity settings that your system controller uses Set the Slave address to an unallocated address number E

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